MD01G1112400.v1.1

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003710
Physical Location & Seq
Reverse (-)
22658861 .. 22662399
3539 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1112400.v1.1.491

Sequence Viewer

Length: 645 bp
ATGGTAGTAAGCATCAAGCCAAAAACCTTCCTCCTTCGCTTTCTCCATCGTCAGATCGCTTCCCTCTCCAACGCGCGCCAAATACAAACACTTTCACCTTCCCCTCTGTCTCTCTACGTATACAGTCCGGCGGGCGGGAGCCATGAGAGCGGCGGCGAGCAGAGCCCCCAGGGAGGAGGAGGAGGAGGAGGCGGTAGCGGCGTCAGGGAGCAGGATAGATATTTGCCAATCGCCAACATCAGTCGCATCATGAAGAAGGCTCTGCCGACCAACGGCAAGATCGCCAAGGACGCCAAGGACACCGTCCAGGAATGCGTCTCCGAATTCATTAGCTTCGTCACCAGCGAGGCCAGCGATAAGTGCCAGAAGGAGAAGCGGAAGACCATCAACGGCGACGATTTGTTGTGGGCGATGGCCACGTTAGGGTTTGAGGACTATATCGAGCCCCTCAGGATTTACTTGGCTAGGTACAGGGAGGGTGATGCAAAGGGGTCTGCTAGGGGTGGAGATGGTTCTTCGAAAGGGAATGCTGTTCCAGCAATGCCTGGCCCAAGTGCACAGTTTGTTCATCAGGGAGCATTGAATTATGGCAACCCTCAAGAGGATTATTATGTGCCATTGAACAATATGGGTAATGGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04612 ko05152 ko05166 map04612 map05152 map05166
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

215

Amino Acids

22.93

Weight (kDa)

8.78

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 74 - 138 7.3e-28 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 150
AccI GTMKAC 1 cut(s) 120
AccII CGCG 2 cut(s) 74, 76
AciI CCGC 7 cut(s) 131, 135, 150, 153, 192, 198, 376
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 1 cut(s) 323
AcyI GRCGYC 2 cut(s) 201, 291
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 5 cut(s) 134, 173, 272, 423, 601
AgsI TTSAA 2 cut(s) 583, 622
AjnI CCWGG 3 cut(s) 168, 306, 544
AluBI AGCT 1 cut(s) 333
AluI AGCT 1 cut(s) 333
Alw21I GWGCWC 1 cut(s) 559
Alw26I GTCTC 2 cut(s) 114, 322
Alw44I GTGCAC 1 cut(s) 555
AoxI GGCC 3 cut(s) 348, 414, 547
ApaLI GTGCAC 1 cut(s) 555
ApoI RAATTY 1 cut(s) 323
AspLEI GCGC 2 cut(s) 76, 78
AspS9I GGNCC 1 cut(s) 548
AsuHPI GGTGA 3 cut(s) 87, 331, 491
AsuII TTCGAA 1 cut(s) 518
AxyI CCTNAGG 1 cut(s) 449
BaeGI GKGCMC 1 cut(s) 559
BalI TGGCCA 1 cut(s) 416
BanII GRGCYC 2 cut(s) 167, 447
BbsI GAAGAC 1 cut(s) 386
Bbv12I GWGCWC 1 cut(s) 559
BccI CCATC 4 cut(s) 54, 392, 406, 503
BceAI ACGGC 2 cut(s) 289, 406
BciT130I CCWGG 3 cut(s) 170, 308, 546
BcoDI GTCTC 2 cut(s) 114, 322
BfaI CTAG 2 cut(s) 465, 498
BisI GCNGC 3 cut(s) 151, 154, 199
BlsI GCNGC 3 cut(s) 152, 155, 200
Bme1390I CCNGG 3 cut(s) 170, 308, 546
BmgT120I GGNCC 1 cut(s) 548
BmiI GGNNCC 1 cut(s) 140
BmrFI CCNGG 3 cut(s) 170, 308, 546
BmsI GCATC 3 cut(s) 21, 255, 472
BpiI GAAGAC 1 cut(s) 386
Bpu14I TTCGAA 1 cut(s) 518
BpuEI CTTGAG 1 cut(s) 582
BsaAI YACGTR 1 cut(s) 118
BsaHI GRCGYC 2 cut(s) 201, 291
BsaJI CCNNGG 4 cut(s) 168, 169, 285, 294
Bsc4I CCNNNNNNNGG 5 cut(s) 134, 173, 272, 423, 601
Bse21I CCTNAGG 1 cut(s) 449
Bse3DI GCAATG 1 cut(s) 546
BseBI CCWGG 3 cut(s) 170, 308, 546
BseDI CCNNGG 4 cut(s) 168, 169, 285, 294
BseLI CCNNNNNNNGG 5 cut(s) 134, 173, 272, 423, 601
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 1 cut(s) 463
BsePI GCGCGC 1 cut(s) 74
BseRI GAGGAG 5 cut(s) 189, 192, 195, 198, 201
BseSI GKGCMC 1 cut(s) 559
Bsh1236I CGCG 2 cut(s) 74, 76
BshFI GGCC 3 cut(s) 350, 416, 549
BsiHKAI GWGCWC 1 cut(s) 559
BsiSI CCGG 1 cut(s) 128
BslI CCNNNNNNNGG 5 cut(s) 134, 173, 272, 423, 601
BsmAI GTCTC 2 cut(s) 114, 322
BsmBI CGTCTC 1 cut(s) 322
BsmI GAATGC 2 cut(s) 317, 532
BsnI GGCC 3 cut(s) 350, 416, 549
Bsp119I TTCGAA 1 cut(s) 518
Bsp1286I GDGCHC 3 cut(s) 167, 447, 559
Bsp143I GATC 2 cut(s) 54, 279
BspACI CCGC 7 cut(s) 131, 135, 150, 153, 192, 198, 376
BspANI GGCC 3 cut(s) 350, 416, 549
BspCNI CTCAG 1 cut(s) 462
BspFNI CGCG 2 cut(s) 74, 76
BspHI TCATGA 1 cut(s) 249
BspLI GGNNCC 1 cut(s) 140
BspT104I TTCGAA 1 cut(s) 518
BsrBI CCGCTC 1 cut(s) 150
BsrDI GCAATG 1 cut(s) 546
BssECI CCNNGG 4 cut(s) 168, 169, 285, 294
BssHII GCGCGC 1 cut(s) 74
BssMI GATC 2 cut(s) 54, 279
BssNAI GTATAC 1 cut(s) 121
BssNI GRCGYC 2 cut(s) 201, 291
BssT1I CCWWGG 2 cut(s) 285, 294
Bst1107I GTATAC 1 cut(s) 121
Bst2UI CCWGG 3 cut(s) 170, 308, 546
Bst4CI ACNGT 3 cut(s) 125, 304, 561
BstACI GRCGYC 2 cut(s) 201, 291
BstBAI YACGTR 1 cut(s) 118
BstBI TTCGAA 1 cut(s) 518
BstC8I GCNNGC 4 cut(s) 76, 133, 158, 352
BstDEI CTNAG 1 cut(s) 449
BstFNI CGCG 2 cut(s) 74, 76
BstHHI GCGC 2 cut(s) 76, 78
BstKTI GATC 2 cut(s) 57, 282
BstMAI GTCTC 2 cut(s) 114, 322
BstMBI GATC 2 cut(s) 54, 279
BstMWI GCNNNNNNNGC 7 cut(s) 147, 162, 198, 290, 351, 360, 536
BstNI CCWGG 3 cut(s) 170, 308, 546
BstSCI CCNGG 3 cut(s) 168, 306, 544
BstSLI GKGCMC 1 cut(s) 559
BstSNI TACGTA 1 cut(s) 118
BstUI CGCG 2 cut(s) 74, 76
BstV2I GAAGAC 1 cut(s) 386
BstZ17I GTATAC 1 cut(s) 121
Bsu36I CCTNAGG 1 cut(s) 449
BsuRI GGCC 3 cut(s) 350, 416, 549
BtgZI GCGATG 1 cut(s) 425
Cac8I GCNNGC 4 cut(s) 76, 133, 158, 352
CciI TCATGA 1 cut(s) 249
CfoI GCGC 2 cut(s) 76, 78
Cfr13I GGNCC 1 cut(s) 548
CseI GACGC 3 cut(s) 190, 299, 304
Csp6I GTAC 1 cut(s) 469
CviAII CATG 2 cut(s) 143, 250
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 1 cut(s) 449
DpnI GATC 2 cut(s) 56, 281
DpnII GATC 2 cut(s) 54, 279
EaeI YGGCCR 1 cut(s) 414
Eco105I TACGTA 1 cut(s) 118
Eco130I CCWWGG 2 cut(s) 285, 294
Eco24I GRGCYC 2 cut(s) 167, 447
Eco81I CCTNAGG 1 cut(s) 449
EcoRI GAATTC 1 cut(s) 323
EcoRII CCWGG 3 cut(s) 168, 306, 544
EcoT14I CCWWGG 2 cut(s) 285, 294
EcoT38I GRGCYC 2 cut(s) 167, 447
ErhI CCWWGG 2 cut(s) 285, 294
Esp3I CGTCTC 1 cut(s) 322
FaeI CATG 2 cut(s) 146, 253
FaiI YATR 7 cut(s) 121, 144, 251, 438, 588, 612, 629
FatI CATG 2 cut(s) 142, 249
FauI CCCGC 2 cut(s) 124, 128
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 3 cut(s) 151, 154, 199
FriOI GRGCYC 2 cut(s) 167, 447
Fsp4HI GCNGC 3 cut(s) 151, 154, 199
FspBI CTAG 2 cut(s) 465, 498
GlaI GCGC 2 cut(s) 75, 77
GluI GCNGC 3 cut(s) 151, 154, 199
HaeIII GGCC 3 cut(s) 350, 416, 549
HapII CCGG 1 cut(s) 128
HgaI GACGC 3 cut(s) 190, 299, 304
HhaI GCGC 2 cut(s) 76, 78
Hin1I GRCGYC 2 cut(s) 201, 291
Hin1II CATG 2 cut(s) 146, 253
Hin6I GCGC 2 cut(s) 74, 76
HinP1I GCGC 2 cut(s) 74, 76
HpaII CCGG 1 cut(s) 128
HphI GGTGA 3 cut(s) 87, 331, 491
Hpy166II GTNNAC 2 cut(s) 121, 557
Hpy188I TCNGA 2 cut(s) 54, 322
Hpy188III TCNNGA 3 cut(s) 250, 451, 599
Hpy8I GTNNAC 2 cut(s) 121, 557
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 5 cut(s) 37, 44, 108, 250, 361
HpyCH4III ACNGT 3 cut(s) 125, 304, 561
HpyCH4IV ACGT 2 cut(s) 117, 419
HpyCH4V TGCA 2 cut(s) 485, 557
HpyF10VI GCNNNNNNNGC 7 cut(s) 147, 162, 198, 290, 351, 360, 536
HpyF3I CTNAG 1 cut(s) 449
HpySE526I ACGT 2 cut(s) 117, 419
Hsp92I GRCGYC 2 cut(s) 201, 291
Hsp92II CATG 2 cut(s) 146, 253
HspAI GCGC 2 cut(s) 74, 76
Kzo9I GATC 2 cut(s) 54, 279
LmnI GCTCC 3 cut(s) 138, 208, 575
LweI GCATC 3 cut(s) 21, 255, 472
MaeI CTAG 2 cut(s) 465, 498
MaeII ACGT 2 cut(s) 117, 419
MaeIII GTNAC 1 cut(s) 337
MalI GATC 2 cut(s) 56, 281
MbiI CCGCTC 1 cut(s) 150
MboI GATC 2 cut(s) 54, 279
MboII GAAGA 3 cut(s) 265, 391, 507
MhlI GDGCHC 3 cut(s) 167, 447, 559
MlsI TGGCCA 1 cut(s) 416
MluCI AATT 2 cut(s) 323, 583
MluNI TGGCCA 1 cut(s) 416
MmeI TCCRAC 1 cut(s) 93
Mox20I TGGCCA 1 cut(s) 416
MscI TGGCCA 1 cut(s) 416
Msp20I TGGCCA 1 cut(s) 416
MspI CCGG 1 cut(s) 128
MspR9I CCNGG 3 cut(s) 170, 308, 546
Mva1269I GAATGC 2 cut(s) 317, 532
MvaI CCWGG 3 cut(s) 170, 308, 546
MvnI CGCG 2 cut(s) 74, 76
MwoI GCNNNNNNNGC 7 cut(s) 147, 162, 198, 290, 351, 360, 536
NdeII GATC 2 cut(s) 54, 279
NlaIII CATG 2 cut(s) 146, 253
NlaIV GGNNCC 1 cut(s) 140
NmuCI GTSAC 1 cut(s) 337
NspV TTCGAA 1 cut(s) 518
PagI TCATGA 1 cut(s) 249
PasI CCCWGGG 1 cut(s) 169
PauI GCGCGC 1 cut(s) 74
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PctI GAATGC 2 cut(s) 317, 532
PflFI GACNNNGTC 1 cut(s) 302
PfoI TCCNGGA 1 cut(s) 306
PkrI GCNGC 3 cut(s) 152, 155, 200
Ppu21I YACGTR 1 cut(s) 118
Psp6I CCWGG 3 cut(s) 168, 306, 544
PspGI CCWGG 3 cut(s) 168, 306, 544
PspN4I GGNNCC 1 cut(s) 140
PspPI GGNCC 1 cut(s) 548
PsyI GACNNNGTC 1 cut(s) 302
PteI GCGCGC 1 cut(s) 74
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
SatI GCNGC 3 cut(s) 151, 154, 199
Sau3AI GATC 2 cut(s) 54, 279
Sau96I GGNCC 1 cut(s) 548
ScrFI CCNGG 3 cut(s) 170, 308, 546
SduI GDGCHC 3 cut(s) 167, 447, 559
SetI ASST 6 cut(s) 29, 100, 120, 335, 422, 470
SfaNI GCATC 3 cut(s) 21, 255, 472
SfuI TTCGAA 1 cut(s) 518
SmlI CTYRAG 1 cut(s) 597
SmoI CTYRAG 1 cut(s) 597
SnaBI TACGTA 1 cut(s) 118
Sse9I AATT 2 cut(s) 323, 583
SsiI CCGC 7 cut(s) 131, 135, 150, 153, 192, 198, 376
SspMI CTAG 2 cut(s) 465, 498
StyD4I CCNGG 3 cut(s) 168, 306, 544
StyI CCWWGG 2 cut(s) 285, 294
TaaI ACNGT 3 cut(s) 125, 304, 561
TaiI ACGT 2 cut(s) 120, 422
TaqI TCGA 2 cut(s) 441, 518
TasI AATT 2 cut(s) 323, 583
TauI GCSGC 3 cut(s) 153, 156, 201
TseFI GTSAC 1 cut(s) 337
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 3 cut(s) 266, 316, 557
Tth111I GACNNNGTC 1 cut(s) 302
VneI GTGCAC 1 cut(s) 555
XapI RAATTY 1 cut(s) 323
XmiI GTMKAC 1 cut(s) 120
XspI CTAG 2 cut(s) 465, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.