MD07G1180200.v1.1

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003710
Physical Location & Seq
Reverse (-)
25950191 .. 25953495
3305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1180200.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGTAAGCATAAAGCCAAAAACCTTCTTCCTTCCCCTTCTCCATCGTCAGATCGCTTCCCTCTCCAACGCGCGCCAAATACAAACACTTTCACCTTCCTCTCTCTCTCTCTCTCTACAAATACACAGTCCGGCTGGCGGGAGCTACGAGAGCGGCGGGGAGCAGAGTCCTCAGAGAGGAGGAGGAGGAGGAGGCGGCGGCAGCGTCCGGGAGCAGGACAGGTATCTGCCAATCGCCAACATCAGTCGCATCATGAAGAAGGCTCTGCCGACCAACGGCAAGATCGCCAAGGACGCCAAGGACACCGTCCAGGAATGCGTCTCCGAATTCATCAGCTTTGTCACCAGCGAGGCAAGCGATAAGTGCCAGAAGGAGAAGCGGAAGACGATCAACGGCGACGATTTGTTGTGGGCGATGGCCACGTTAGGGTTTGAGGACTATATCGAGCCCCTCAGGATTTACTTGAGTAGGTACAGGGAGGGTGATGCAAAGGGCTCAGCTAGGGGTGGAGATGGTTCTTTTAAAGGGAATGCTGTTCCAGCAATGCCTGGCCCAAGTTCACAGTTTGTCCATCAGGGAGCATTGAACTATGGCAACCCTCAAGAGGATTATTATGTGCCCTTGAATAATATGGGAAATGGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04612 ko05152 ko05166 map04612 map05152 map05166
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

216

Amino Acids

23.16

Weight (kDa)

8.8

Isoelectric Point (pI)

50.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 75 - 139 7.4e-28 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 153
AccII CGCG 2 cut(s) 71, 73
AciI CCGC 6 cut(s) 138, 153, 156, 195, 198, 379
AcoI YGGCCR 1 cut(s) 417
AcsI RAATTY 1 cut(s) 326
AcyI GRCGYC 1 cut(s) 294
AfaI GTAC 1 cut(s) 473
AfiI CCNNNNNNNGG 6 cut(s) 137, 176, 214, 275, 426, 604
AgsI TTSAA 2 cut(s) 586, 625
AjnI CCWGG 2 cut(s) 309, 547
AluBI AGCT 3 cut(s) 144, 336, 500
AluI AGCT 3 cut(s) 144, 336, 500
Alw26I GTCTC 1 cut(s) 325
AoxI GGCC 2 cut(s) 417, 550
ApeKI GCWGC 1 cut(s) 201
ApoI RAATTY 1 cut(s) 326
ArsI GACNNNNNNTTYG 2 cut(s) 112, 144
AspLEI GCGC 2 cut(s) 73, 75
AspS9I GGNCC 1 cut(s) 551
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 3 cut(s) 84, 334, 494
AxyI CCTNAGG 1 cut(s) 452
BaeGI GKGCMC 1 cut(s) 621
BalI TGGCCA 1 cut(s) 419
BanII GRGCYC 2 cut(s) 450, 497
BbsI GAAGAC 1 cut(s) 389
BbvI GCAGC 1 cut(s) 213
BccI CCATC 4 cut(s) 51, 409, 506, 579
BceAI ACGGC 2 cut(s) 292, 409
BciT130I CCWGG 2 cut(s) 311, 549
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 1 cut(s) 325
BfaI CTAG 1 cut(s) 501
BisI GCNGC 4 cut(s) 154, 196, 199, 202
BlpI GCTNAGC 1 cut(s) 496
BlsI GCNGC 4 cut(s) 155, 197, 200, 203
Bme1390I CCNGG 3 cut(s) 209, 311, 549
BmgT120I GGNCC 1 cut(s) 551
BmrFI CCNGG 3 cut(s) 209, 311, 549
BmsI GCATC 2 cut(s) 258, 475
BpiI GAAGAC 1 cut(s) 389
Bpu1102I GCTNAGC 1 cut(s) 496
BpuEI CTTGAG 2 cut(s) 484, 585
BpuMI CCSGG 1 cut(s) 209
BsaHI GRCGYC 1 cut(s) 294
BsaJI CCNNGG 2 cut(s) 288, 297
Bsc4I CCNNNNNNNGG 6 cut(s) 137, 176, 214, 275, 426, 604
Bse21I CCTNAGG 1 cut(s) 452
Bse3DI GCAATG 1 cut(s) 549
BseBI CCWGG 2 cut(s) 311, 549
BseDI CCNNGG 2 cut(s) 288, 297
BseLI CCNNNNNNNGG 6 cut(s) 137, 176, 214, 275, 426, 604
BseMI GCAATG 1 cut(s) 549
BseMII CTCAG 3 cut(s) 185, 466, 510
BsePI GCGCGC 1 cut(s) 71
BseRI GAGGAG 5 cut(s) 192, 195, 198, 201, 204
BseSI GKGCMC 1 cut(s) 621
BseXI GCAGC 1 cut(s) 213
Bsh1236I CGCG 2 cut(s) 71, 73
BshFI GGCC 2 cut(s) 419, 552
BsiSI CCGG 2 cut(s) 131, 208
BslI CCNNNNNNNGG 6 cut(s) 137, 176, 214, 275, 426, 604
BsmAI GTCTC 1 cut(s) 325
BsmBI CGTCTC 1 cut(s) 325
BsmI GAATGC 2 cut(s) 320, 535
BsnI GGCC 2 cut(s) 419, 552
Bsp1286I GDGCHC 3 cut(s) 450, 497, 621
Bsp143I GATC 3 cut(s) 51, 282, 387
Bsp1720I GCTNAGC 1 cut(s) 496
BspACI CCGC 6 cut(s) 138, 153, 156, 195, 198, 379
BspANI GGCC 2 cut(s) 419, 552
BspCNI CTCAG 3 cut(s) 184, 465, 509
BspFNI CGCG 2 cut(s) 71, 73
BspHI TCATGA 1 cut(s) 252
BsrBI CCGCTC 1 cut(s) 153
BsrDI GCAATG 1 cut(s) 549
BssECI CCNNGG 2 cut(s) 288, 297
BssHII GCGCGC 1 cut(s) 71
BssMI GATC 3 cut(s) 51, 282, 387
BssNI GRCGYC 1 cut(s) 294
BssT1I CCWWGG 2 cut(s) 288, 297
Bst2UI CCWGG 2 cut(s) 311, 549
Bst4CI ACNGT 3 cut(s) 128, 307, 564
BstACI GRCGYC 1 cut(s) 294
BstC8I GCNNGC 3 cut(s) 73, 136, 355
BstDEI CTNAG 3 cut(s) 171, 452, 496
BstENI CCTNNNNNAGG 1 cut(s) 174
BstFNI CGCG 2 cut(s) 71, 73
BstHHI GCGC 2 cut(s) 73, 75
BstKTI GATC 3 cut(s) 54, 285, 390
BstMAI GTCTC 1 cut(s) 325
BstMBI GATC 3 cut(s) 51, 282, 387
BstMWI GCNNNNNNNGC 6 cut(s) 150, 201, 293, 354, 363, 539
BstNI CCWGG 2 cut(s) 311, 549
BstSCI CCNGG 3 cut(s) 207, 309, 547
BstSLI GKGCMC 1 cut(s) 621
BstUI CGCG 2 cut(s) 71, 73
BstV1I GCAGC 1 cut(s) 213
BstV2I GAAGAC 1 cut(s) 389
Bsu36I CCTNAGG 1 cut(s) 452
BsuRI GGCC 2 cut(s) 419, 552
BtgZI GCGATG 1 cut(s) 428
Cac8I GCNNGC 3 cut(s) 73, 136, 355
CciI TCATGA 1 cut(s) 252
CfoI GCGC 2 cut(s) 73, 75
Cfr13I GGNCC 1 cut(s) 551
CseI GACGC 3 cut(s) 193, 302, 307
Csp6I GTAC 1 cut(s) 472
CviAII CATG 1 cut(s) 253
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 3 cut(s) 171, 452, 496
DpnI GATC 3 cut(s) 53, 284, 389
DpnII GATC 3 cut(s) 51, 282, 387
DraI TTTAAA 1 cut(s) 523
EaeI YGGCCR 1 cut(s) 417
Eco130I CCWWGG 2 cut(s) 288, 297
Eco24I GRGCYC 2 cut(s) 450, 497
Eco81I CCTNAGG 1 cut(s) 452
EcoNI CCTNNNNNAGG 1 cut(s) 174
EcoRI GAATTC 1 cut(s) 326
EcoRII CCWGG 2 cut(s) 309, 547
EcoT14I CCWWGG 2 cut(s) 288, 297
EcoT38I GRGCYC 2 cut(s) 450, 497
ErhI CCWWGG 2 cut(s) 288, 297
Esp3I CGTCTC 1 cut(s) 325
FaeI CATG 1 cut(s) 256
FaiI YATR 6 cut(s) 11, 254, 441, 591, 615, 632
FatI CATG 1 cut(s) 252
FauI CCCGC 2 cut(s) 131, 149
Fnu4HI GCNGC 4 cut(s) 154, 196, 199, 202
FriOI GRGCYC 2 cut(s) 450, 497
Fsp4HI GCNGC 4 cut(s) 154, 196, 199, 202
FspBI CTAG 1 cut(s) 501
GlaI GCGC 2 cut(s) 72, 74
GluI GCNGC 4 cut(s) 154, 196, 199, 202
HaeIII GGCC 2 cut(s) 419, 552
HapII CCGG 2 cut(s) 131, 208
HgaI GACGC 3 cut(s) 193, 302, 307
HhaI GCGC 2 cut(s) 73, 75
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 1 cut(s) 256
Hin6I GCGC 2 cut(s) 71, 73
HinP1I GCGC 2 cut(s) 71, 73
HinfI GANTC 1 cut(s) 166
HpaII CCGG 2 cut(s) 131, 208
HphI GGTGA 3 cut(s) 84, 334, 494
Hpy166II GTNNAC 1 cut(s) 560
Hpy188I TCNGA 3 cut(s) 51, 174, 325
Hpy188III TCNNGA 3 cut(s) 253, 454, 602
Hpy8I GTNNAC 1 cut(s) 560
Hpy99I CGWCG 1 cut(s) 401
HpyAV CCTTC 6 cut(s) 34, 41, 47, 105, 253, 364
HpyCH4III ACNGT 3 cut(s) 128, 307, 564
HpyCH4IV ACGT 1 cut(s) 422
HpyCH4V TGCA 1 cut(s) 488
HpyF10VI GCNNNNNNNGC 6 cut(s) 150, 201, 293, 354, 363, 539
HpyF3I CTNAG 3 cut(s) 171, 452, 496
HpySE526I ACGT 1 cut(s) 422
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 1 cut(s) 256
HspAI GCGC 2 cut(s) 71, 73
Kzo9I GATC 3 cut(s) 51, 282, 387
LmnI GCTCC 4 cut(s) 141, 160, 211, 578
Lsp1109I GCAGC 1 cut(s) 213
LweI GCATC 2 cut(s) 258, 475
MaeI CTAG 1 cut(s) 501
MaeII ACGT 1 cut(s) 422
MaeIII GTNAC 1 cut(s) 340
MalI GATC 3 cut(s) 53, 284, 389
MbiI CCGCTC 1 cut(s) 153
MboI GATC 3 cut(s) 51, 282, 387
MboII GAAGA 3 cut(s) 19, 268, 394
MhlI GDGCHC 3 cut(s) 450, 497, 621
MlsI TGGCCA 1 cut(s) 419
MluCI AATT 2 cut(s) 326, 643
MluNI TGGCCA 1 cut(s) 419
MlyI GAGTC 1 cut(s) 175
MmeI TCCRAC 1 cut(s) 90
Mox20I TGGCCA 1 cut(s) 419
MscI TGGCCA 1 cut(s) 419
MseI TTAA 1 cut(s) 522
Msp20I TGGCCA 1 cut(s) 419
MspI CCGG 2 cut(s) 131, 208
MspR9I CCNGG 3 cut(s) 209, 311, 549
Mva1269I GAATGC 2 cut(s) 320, 535
MvaI CCWGG 2 cut(s) 311, 549
MvnI CGCG 2 cut(s) 71, 73
MwoI GCNNNNNNNGC 6 cut(s) 150, 201, 293, 354, 363, 539
NciI CCSGG 1 cut(s) 209
NdeII GATC 3 cut(s) 51, 282, 387
NlaIII CATG 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 340
PagI TCATGA 1 cut(s) 252
PauI GCGCGC 1 cut(s) 71
PctI GAATGC 2 cut(s) 320, 535
PflFI GACNNNGTC 1 cut(s) 305
PfoI TCCNGGA 2 cut(s) 207, 309
PkrI GCNGC 4 cut(s) 155, 197, 200, 203
PleI GAGTC 1 cut(s) 174
PpsI GAGTC 1 cut(s) 174
Psp6I CCWGG 2 cut(s) 309, 547
PspGI CCWGG 2 cut(s) 309, 547
PspPI GGNCC 1 cut(s) 551
PsyI GACNNNGTC 1 cut(s) 305
PteI GCGCGC 1 cut(s) 71
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
SaqAI TTAA 1 cut(s) 522
SatI GCNGC 4 cut(s) 154, 196, 199, 202
Sau3AI GATC 3 cut(s) 51, 282, 387
Sau96I GGNCC 1 cut(s) 551
SchI GAGTC 1 cut(s) 175
ScrFI CCNGG 3 cut(s) 209, 311, 549
SduI GDGCHC 3 cut(s) 450, 497, 621
SetI ASST 8 cut(s) 26, 97, 146, 224, 338, 425, 473, 502
SfaNI GCATC 2 cut(s) 258, 475
SmlI CTYRAG 2 cut(s) 463, 600
SmoI CTYRAG 2 cut(s) 463, 600
Sse9I AATT 2 cut(s) 326, 643
SsiI CCGC 6 cut(s) 138, 153, 156, 195, 198, 379
SspMI CTAG 1 cut(s) 501
StyD4I CCNGG 3 cut(s) 207, 309, 547
StyI CCWWGG 2 cut(s) 288, 297
TaaI ACNGT 3 cut(s) 128, 307, 564
TaiI ACGT 1 cut(s) 425
TaqI TCGA 1 cut(s) 444
TasI AATT 2 cut(s) 326, 643
TauI GCSGC 3 cut(s) 156, 198, 201
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 1 cut(s) 201
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 2 cut(s) 269, 319
Tth111I GACNNNGTC 1 cut(s) 305
XagI CCTNNNNNAGG 1 cut(s) 174
XapI RAATTY 1 cut(s) 326
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.