Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0005770

Physical Location & Seq

Genomic Coordinates Forward (+)

23768792 .. 23773246

Gene Structure 4455 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1125100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1821 bp

ATGGCGGCGGCGGCAGCAGCGGCGAGAATAAACCTCTTCTTAAACCCCACAAACCCCCTCAATTACAAAGCCACCGCCCATTTCAGAACCCACCTCCGCTTCCAAAGAAGAAGAACCAAAGCTACGTCTTTCTGCACCCGCGCCTCCGCTGCTCCCCGCCACGACGATGCATCGGGCGCCGGAGGCAGTGACCCTTTTGTCCTCACCACTCCGCTCTATTACGTGAACGCCCCTCCCCATTTGGGTAGCGCTTACACCACCATCGCCGCCGACGCCATTGCTCGCTTCCAGAGGCTTTTGGGTAAGAACGTCATTTTCGTCACCGGCACTGACGAGCACGGTGAGAAGATTGCCGCTGCCGCCGCTGCTAATGCAGCCGCCCCAGCTCAACACTGTGACCTCATTTCACAATCTTACATTTCGCTCTGGAAACAGTTAGATATATCTTATGACAAGTTCATTCGGACTACCGACCCCAAGCATGAGGCAATTGTCAAGGAATTTTACTCCAGAGTTCTTGCCAATGGAGACATTTACAGGGCTGACTACGAGGGACTTTACTGTGTTAACTGTGAAGAGTATAAGGATGAGAAAGAACTGCTTGAGAACAACTTTTGCCCTACGCACCTAAAGCCATGTGTTGCACGAAAAGAGGATAATTACTTTTTTGCATTGTCCAAGTATCAAAAATCGTTGGAAGAAACATTGGCAACAAATCAAGAATTTGTACAGCCTCCATTCCGTCTTAATGAGGTTCAAGGTTGGGTTAAAAGTGGCCTAAGAGACTTTTCCATTTCTCGAGCATTAGTGGATTGGGGCATCCCCGTTCCTAATGACAGCAAGCAGACAATATATGTTTGGTTTGATGCTCTACTGGGTTATATATCAGCACTATCAGAGGATATGGAGCAACCCAATTTGGAAAGAGCCGTCTCATCAGGGTGGCCTGCCTCACTACACTTGATTGGCAAGGATATTTTACGTTTCCATGCAGTTTACTGGCCAGCTATGCTTATGTCTGCGGGACTAGATCTTCCTAAGATGGTGTTTGGTCATGGATTCTTGACAAAGGATGGTATGAAAATGGGGAAGTCGCTAGGGAATACGATTGAACCAAATGATTTGGTTCAGAAATTCGGGTCTGATGCAGTGAGGTACTTCTTCCTAAGAGAGGTGGAATTCGGAAATGATGGGGACTATTCAGAAGAACGTTTCATCAATATTGTCAATGCTCATCTTGCAAATTCAATTGGAAACCTGCTTAACCGTACCCTGGGACTTCTTAAGAAAAACTGCCAATCAACTTTGGCAGTTGATTCAAGTATTGCAGTTGAAGGAACTACATTCAAGGACACAGTGGAGAAATTGGTTGAAAAAGCTCGGGTTCATTATGGAGACCTCTCTCTGTCATCAGCTTGTGAGGCTGTGCTAGAGATCAGCCATACTGGAAATTTATACATGGATGAGCGTGCACCATGGTCACTCTTTAAGCAAGGGGGTGCTGCTTCTGAAGCTGCCGCGAAGGACCTTGTGATAATACTAGAAACAATGAGGATTATAGCAATTGCATTATCACCTGTCACGCCGAGCTTAAGTTGGAGAATATATGGACAGCTTGGCTACTCAAAGGATCAGTTCGATGCTGCTACCTGGAGCGACACCAAGTGGGGCGGGCTAAAGAGTGGTCAGGTCATGGCGCAGCCTAAACCGATCTTTGCAAGGATTGAAAACCCAACGGAAGAAGAAAACGGGAAGGAAACACCTAAAAAGGCGGTAAAAAAGCAGAAGCTACCCCAAGACAAACGGGCAGTAGAAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004825 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006431 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0035670 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00450 ko00970 map00450 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1g

Protein Analysis

607

Amino Acids

67.35

Weight (kDa)

6.72

Isoelectric Point (pI)

32.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	63 - 277	2.8e-11	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	67 - 200	1.6e-41	tRNA synthetases class I (M)
tRNA-synt_1e	PF01406	78 - 233	6.4e-07	tRNA synthetases class I (C) catalytic domain
tRNA-synt_1g	PF09334	201 - 425	5.7e-64	tRNA synthetases class I (M)
tRNA-synt_1	PF00133	283 - 391	1.9e-11	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1e	PF01406	344 - 405	8e-06	tRNA synthetases class I (C) catalytic domain
Anticodon_3	PF19303	447 - 582	3.9e-10	Anticodon binding domain of methionyl tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005770)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55400 AT3G55400
fragaria_vesca	FvH4_6g24170 FvH4_6g24170 FvH4_6g51210 FvH4_6g51210 FvH4_6g51210 FvH4_6g51210
malus_domestica	MD01G1125100.v1.1 MD07G1194800.v1.1
prunus_persica	Prupe.2G232400_v2.0.a1 Prupe.2G232400_v2.0.a1
pyrus_communis	pycom01g15130 pycom07g18350 pycom07g18360
rosa_chinensis	RchiOBHm_Chr3g0482991 RchiOBHm_Chr3g0483011
rosa_laevigata	RLG00000023307 RLG00000023309
rosa_multiflora	Rmu_sc0003351.1_g000014
rosa_roxburghii	Rroxscaffold_6G00398740
rosa_rugosa	Rorug03G0202800
rosa_samantha	Rh3AG251600 Rh3BG288100 Rh3CG285600 Rh3DG281300
rosa_wichuraiana	Rw3G022730 Rw3G022760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	197
Acc36I	ACCTGC	1 cut(s)	1266
AccB1I	GGYRCC	1 cut(s)	176
AccBSI	CCGCTC	1 cut(s)	214
AccII	CGCG	2 cut(s)	141, 1520
AclI	AACGTT	1 cut(s)	1210
AclWI	GGATC	1 cut(s)	1638
AcoI	YGGCCR	1 cut(s)	1001
AcsI	RAATTY	6 cut(s)	500, 722, 1133, 1178, 1243, 1450
AcuI	CTGAAG	1 cut(s)	1530
AcyI	GRCGYC	2 cut(s)	177, 273
AdeI	CACNNNGTG	1 cut(s)	1665
AfaI	GTAC	3 cut(s)	729, 1157, 1270
AfeI	AGCGCT	1 cut(s)	250
AfiI	CCNNNNNNNGG	3 cut(s)	242, 1171, 1273
AflII	CTTAAG	2 cut(s)	1283, 1591
AgsI	TTSAA	8 cut(s)	758, 1112, 1248, 1320, 1334, 1348, 1373, 1727
AjnI	CCWGG	2 cut(s)	1272, 1649
Alw21I	GWGCWC	2 cut(s)	339, 1474
Alw26I	GTCTC	4 cut(s)	522, 777, 937, 1389
Alw44I	GTGCAC	1 cut(s)	1470
AlwI	GGATC	1 cut(s)	1638
Ama87I	CYCGRG	2 cut(s)	798, 1380
Aor51HI	AGCGCT	1 cut(s)	250
AoxI	GGCC	3 cut(s)	775, 944, 1001
ApaLI	GTGCAC	1 cut(s)	1470
ApoI	RAATTY	6 cut(s)	500, 722, 1133, 1178, 1243, 1450
AspLEI	GCGC	4 cut(s)	143, 179, 251, 1699
AspS9I	GGNCC	1 cut(s)	1525
AsuHPI	GGTGA	4 cut(s)	196, 313, 353, 1566
AvaI	CYCGRG	2 cut(s)	798, 1380
AvaII	GGWCC	1 cut(s)	1525
BaeGI	GKGCMC	1 cut(s)	1474
BalI	TGGCCA	1 cut(s)	1003
BanI	GGYRCC	1 cut(s)	176
Bbv12I	GWGCWC	2 cut(s)	339, 1474
BccI	CCATC	4 cut(s)	269, 1036, 1067, 1184
BceAI	ACGGC	1 cut(s)	914
BcgI	CGANNNNNNTGC	2 cut(s)	260, 294
BciT130I	CCWGG	2 cut(s)	1274, 1651
BcoDI	GTCTC	4 cut(s)	522, 777, 937, 1389
BfaI	CTAG	4 cut(s)	1028, 1097, 1430, 1541
BfoI	RGCGCY	2 cut(s)	180, 252
BfrI	CTTAAG	2 cut(s)	1283, 1591
BfuAI	ACCTGC	1 cut(s)	1266
BglII	AGATCT	1 cut(s)	1030
Bme1390I	CCNGG	2 cut(s)	1274, 1651
Bme18I	GGWCC	1 cut(s)	1525
BmeT110I	CYCGRG	2 cut(s)	798, 1380
BmgT120I	GGNCC	1 cut(s)	1525
BmiI	GGNNCC	1 cut(s)	178
BmrFI	CCNGG	2 cut(s)	1274, 1651
BmrI	ACTGGG	1 cut(s)	884
BmsI	GCATC	6 cut(s)	157, 179, 828, 856, 1135, 1630
BmuI	ACTGGG	1 cut(s)	884
BpmI	CTGGAG	2 cut(s)	493, 1672
BpuEI	CTTGAG	1 cut(s)	623
BsaAI	YACGTR	1 cut(s)	223
BsaHI	GRCGYC	2 cut(s)	177, 273
BsaI	GGTCTC	1 cut(s)	1389
BsaJI	CCNNGG	3 cut(s)	1272, 1273, 1475
Bsc4I	CCNNNNNNNGG	3 cut(s)	242, 1171, 1273
Bse118I	RCCGGY	1 cut(s)	323
Bse1I	ACTGG	3 cut(s)	879, 1004, 1450
Bse3DI	GCAATG	1 cut(s)	276
BseBI	CCWGG	2 cut(s)	1274, 1651
BseDI	CCNNGG	3 cut(s)	1272, 1273, 1475
BseGI	GGATG	4 cut(s)	592, 819, 1078, 1468
BseLI	CCNNNNNNNGG	3 cut(s)	242, 1171, 1273
BseMI	GCAATG	1 cut(s)	276
BseNI	ACTGG	3 cut(s)	879, 1004, 1450
BseSI	GKGCMC	1 cut(s)	1474
BseYI	CCCAGC	1 cut(s)	382
BsgI	GTGCAG	1 cut(s)	118
Bsh1236I	CGCG	2 cut(s)	141, 1520
BshFI	GGCC	3 cut(s)	777, 946, 1003
BshNI	GGYRCC	1 cut(s)	176
BsiHKAI	GWGCWC	2 cut(s)	339, 1474
BsiHKCI	CYCGRG	2 cut(s)	798, 1380
BsiSI	CCGG	2 cut(s)	180, 324
BslFI	GGGAC	4 cut(s)	567, 1038, 1208, 1290
BslI	CCNNNNNNNGG	3 cut(s)	242, 1171, 1273
BsmAI	GTCTC	4 cut(s)	522, 777, 937, 1389
BsmBI	CGTCTC	1 cut(s)	937
BsmFI	GGGAC	4 cut(s)	567, 1038, 1208, 1290
BsnI	GGCC	3 cut(s)	777, 946, 1003
Bso31I	GGTCTC	1 cut(s)	1389
BsoBI	CYCGRG	2 cut(s)	798, 1380
Bsp1286I	GDGCHC	2 cut(s)	339, 1474
Bsp1407I	TGTACA	1 cut(s)	727
Bsp143I	GATC	4 cut(s)	1030, 1434, 1630, 1710
Bsp19I	CCATGG	1 cut(s)	1475
BspANI	GGCC	3 cut(s)	777, 946, 1003
BspFNI	CGCG	2 cut(s)	141, 1520
BspLI	GGNNCC	1 cut(s)	178
BspMI	ACCTGC	1 cut(s)	1266
BspPI	GGATC	1 cut(s)	1638
BspT107I	GGYRCC	1 cut(s)	176
BspTI	CTTAAG	2 cut(s)	1283, 1591
BspTNI	GGTCTC	1 cut(s)	1389
BsrBI	CCGCTC	1 cut(s)	214
BsrDI	GCAATG	1 cut(s)	276
BsrFI	RCCGGY	1 cut(s)	323
BsrGI	TGTACA	1 cut(s)	727
BsrI	ACTGG	3 cut(s)	879, 1004, 1450
BssAI	RCCGGY	1 cut(s)	323
BssECI	CCNNGG	3 cut(s)	1272, 1273, 1475
BssMI	GATC	4 cut(s)	1030, 1434, 1630, 1710
BssNI	GRCGYC	2 cut(s)	177, 273
BssT1I	CCWWGG	1 cut(s)	1475
Bst2UI	CCWGG	2 cut(s)	1274, 1651
Bst4CI	ACNGT	7 cut(s)	341, 395, 435, 563, 572, 1268, 1357
Bst6I	CTCTTC	2 cut(s)	41, 570
BstACI	GRCGYC	2 cut(s)	177, 273
BstAFI	CTTAAG	2 cut(s)	1283, 1591
BstAUI	TGTACA	1 cut(s)	727
BstBAI	YACGTR	1 cut(s)	223
BstC8I	GCNNGC	6 cut(s)	283, 842, 948, 1005, 1470, 1673
BstDEI	CTNAG	3 cut(s)	779, 1038, 1166
BstDSI	CCRYGG	1 cut(s)	1475
BstENI	CCTNNNNNAGG	1 cut(s)	1169
BstF5I	GGATG	4 cut(s)	592, 819, 1078, 1468
BstFNI	CGCG	2 cut(s)	141, 1520
BstH2I	RGCGCY	2 cut(s)	180, 252
BstHHI	GCGC	4 cut(s)	143, 179, 251, 1699
BstKTI	GATC	4 cut(s)	1033, 1437, 1633, 1713
BstMAI	GTCTC	4 cut(s)	522, 777, 937, 1389
BstMBI	GATC	4 cut(s)	1030, 1434, 1630, 1710
BstNI	CCWGG	2 cut(s)	1274, 1651
BstSCI	CCNGG	2 cut(s)	1272, 1649
BstSLI	GKGCMC	1 cut(s)	1474
BstUI	CGCG	2 cut(s)	141, 1520
BstX2I	RGATCY	1 cut(s)	1030
BstYI	RGATCY	1 cut(s)	1030
BsuRI	GGCC	3 cut(s)	777, 946, 1003
BtgI	CCRYGG	1 cut(s)	1475
BtgZI	GCGATG	1 cut(s)	247
BtsCI	GGATG	4 cut(s)	592, 819, 1078, 1468
BtsI	GCAGTG	2 cut(s)	193, 1155
BtsIMutI	CAGTG	5 cut(s)	193, 327, 391, 1155, 1362
BveI	ACCTGC	1 cut(s)	1266
Cac8I	GCNNGC	6 cut(s)	283, 842, 948, 1005, 1470, 1673
CfoI	GCGC	4 cut(s)	143, 179, 251, 1699
Cfr10I	RCCGGY	1 cut(s)	323
Cfr13I	GGNCC	1 cut(s)	1525
CseI	GACGC	1 cut(s)	281
Csp6I	GTAC	3 cut(s)	728, 1156, 1269
CviAII	CATG	7 cut(s)	482, 636, 989, 1055, 1459, 1476, 1693
CviQI	GTAC	3 cut(s)	728, 1156, 1269
DdeI	CTNAG	3 cut(s)	779, 1038, 1166
DinI	GGCGCC	1 cut(s)	178
DpnI	GATC	4 cut(s)	1032, 1436, 1632, 1712
DpnII	GATC	4 cut(s)	1030, 1434, 1630, 1710
DraIII	CACNNNGTG	1 cut(s)	1665
DrdI	GACNNNNNNGTC	1 cut(s)	197
DseDI	GACNNNNNNGTC	1 cut(s)	197
EaeI	YGGCCR	1 cut(s)	1001
Eam1104I	CTCTTC	2 cut(s)	41, 570
EarI	CTCTTC	2 cut(s)	41, 570
Eco130I	CCWWGG	1 cut(s)	1475
Eco31I	GGTCTC	1 cut(s)	1389
Eco47I	GGWCC	1 cut(s)	1525
Eco47III	AGCGCT	1 cut(s)	250
Eco57I	CTGAAG	1 cut(s)	1530
Eco88I	CYCGRG	2 cut(s)	798, 1380
EcoNI	CCTNNNNNAGG	1 cut(s)	1169
EcoO109I	RGGNCCY	1 cut(s)	1525
EcoRI	GAATTC	1 cut(s)	1178
EcoRII	CCWGG	2 cut(s)	1272, 1649
EcoT14I	CCWWGG	1 cut(s)	1475
EcoT22I	ATGCAT	1 cut(s)	172
EgeI	GGCGCC	1 cut(s)	178
EheI	GGCGCC	1 cut(s)	178
ErhI	CCWWGG	1 cut(s)	1475
Esp3I	CGTCTC	1 cut(s)	937
FaeI	CATG	7 cut(s)	485, 639, 992, 1058, 1462, 1479, 1696
FalI	AAGNNNNNCTT	2 cut(s)	585, 617
FaqI	GGGAC	4 cut(s)	567, 1038, 1208, 1290
FatI	CATG	7 cut(s)	481, 635, 988, 1054, 1458, 1475, 1692
FauI	CCCGC	4 cut(s)	146, 164, 1015, 1664
FokI	GGATG	4 cut(s)	599, 806, 1085, 1475
FspBI	CTAG	4 cut(s)	1028, 1097, 1430, 1541
GlaI	GCGC	4 cut(s)	142, 178, 250, 1698
GsaI	CCCAGC	1 cut(s)	386
GsuI	CTGGAG	2 cut(s)	493, 1672
HaeII	RGCGCY	2 cut(s)	180, 252
HaeIII	GGCC	3 cut(s)	777, 946, 1003
HapII	CCGG	2 cut(s)	180, 324
HgaI	GACGC	1 cut(s)	281
HhaI	GCGC	4 cut(s)	143, 179, 251, 1699
Hin1I	GRCGYC	2 cut(s)	177, 273
Hin1II	CATG	7 cut(s)	485, 639, 992, 1058, 1462, 1479, 1696
Hin6I	GCGC	4 cut(s)	141, 177, 249, 1697
HinP1I	GCGC	4 cut(s)	141, 177, 249, 1697
HincII	GTYRAC	1 cut(s)	568
HindII	GTYRAC	1 cut(s)	568
HindIII	AAGCTT	1 cut(s)	1815
HinfI	GANTC	2 cut(s)	1059, 1316
HpaI	GTTAAC	1 cut(s)	568
HpaII	CCGG	2 cut(s)	180, 324
HphI	GGTGA	4 cut(s)	196, 313, 353, 1566
Hpy166II	GTNNAC	4 cut(s)	226, 568, 997, 1472
Hpy188I	TCNGA	8 cut(s)	86, 465, 898, 1131, 1144, 1184, 1204, 1510
Hpy188III	TCNNGA	6 cut(s)	289, 427, 510, 719, 798, 1063
Hpy8I	GTNNAC	4 cut(s)	226, 568, 997, 1472
Hpy99I	CGWCG	2 cut(s)	167, 275
HpyAV	CCTTC	3 cut(s)	1328, 1516, 1747
HpyCH4III	ACNGT	7 cut(s)	341, 395, 435, 563, 572, 1268, 1357
HpyCH4IV	ACGT	5 cut(s)	125, 222, 309, 982, 1210
HpyF3I	CTNAG	3 cut(s)	779, 1038, 1166
HpySE526I	ACGT	5 cut(s)	125, 222, 309, 982, 1210
Hsp92I	GRCGYC	2 cut(s)	177, 273
Hsp92II	CATG	7 cut(s)	485, 639, 992, 1058, 1462, 1479, 1696
HspAI	GCGC	4 cut(s)	141, 177, 249, 1697
KasI	GGCGCC	1 cut(s)	176
KspAI	GTTAAC	1 cut(s)	568
Kzo9I	GATC	4 cut(s)	1030, 1434, 1630, 1710
LmnI	GCTCC	3 cut(s)	157, 907, 1653
LweI	GCATC	6 cut(s)	157, 179, 828, 856, 1135, 1630
MaeI	CTAG	4 cut(s)	1028, 1097, 1430, 1541
MaeII	ACGT	5 cut(s)	125, 222, 309, 982, 1210
MaeIII	GTNAC	5 cut(s)	188, 319, 395, 1479, 1579
MalI	GATC	4 cut(s)	1032, 1436, 1632, 1712
MbiI	CCGCTC	1 cut(s)	214
MboI	GATC	4 cut(s)	1030, 1434, 1630, 1710
MfeI	CAATTG	3 cut(s)	489, 1248, 1563
MflI	RGATCY	1 cut(s)	1030
MhlI	GDGCHC	2 cut(s)	339, 1474
MlsI	TGGCCA	1 cut(s)	1003
MluNI	TGGCCA	1 cut(s)	1003
Mly113I	GGCGCC	1 cut(s)	177
MmeI	TCCRAC	2 cut(s)	675, 1577
Mox20I	TGGCCA	1 cut(s)	1003
Mph1103I	ATGCAT	1 cut(s)	172
MscI	TGGCCA	1 cut(s)	1003
MseI	TTAA	9 cut(s)	41, 567, 747, 768, 1263, 1284, 1488, 1592, 1819
MslI	CAYNNNNRTG	2 cut(s)	165, 940
Msp20I	TGGCCA	1 cut(s)	1003
MspA1I	CMGCKG	4 cut(s)	20, 149, 356, 365
MspCI	CTTAAG	2 cut(s)	1283, 1591
MspI	CCGG	2 cut(s)	180, 324
MspR9I	CCNGG	2 cut(s)	1274, 1651
MunI	CAATTG	3 cut(s)	489, 1248, 1563
MvaI	CCWGG	2 cut(s)	1274, 1651
MvnI	CGCG	2 cut(s)	141, 1520
NarI	GGCGCC	1 cut(s)	177
NcoI	CCATGG	1 cut(s)	1475
NdeII	GATC	4 cut(s)	1030, 1434, 1630, 1710
NlaIII	CATG	7 cut(s)	485, 639, 992, 1058, 1462, 1479, 1696
NlaIV	GGNNCC	1 cut(s)	178
NmeAIII	GCCGAG	1 cut(s)	1611
NmuCI	GTSAC	5 cut(s)	188, 319, 395, 1479, 1579
NsiI	ATGCAT	1 cut(s)	172
PaeR7I	CTCGAG	1 cut(s)	798
PasI	CCCWGGG	1 cut(s)	1273
PcsI	WCGNNNNNNNCGW	1 cut(s)	315
PfeI	GAWTC	2 cut(s)	1059, 1316
PluTI	GGCGCC	1 cut(s)	180
Ppu21I	YACGTR	1 cut(s)	223
PpuMI	RGGWCCY	1 cut(s)	1525
Psp1406I	AACGTT	1 cut(s)	1210
Psp5II	RGGWCCY	1 cut(s)	1525
Psp6I	CCWGG	2 cut(s)	1272, 1649
PspFI	CCCAGC	1 cut(s)	382
PspGI	CCWGG	2 cut(s)	1272, 1649
PspN4I	GGNNCC	1 cut(s)	178
PspPI	GGNCC	1 cut(s)	1525
PspPPI	RGGWCCY	1 cut(s)	1525
PsrI	GAACNNNNNNTAC	2 cut(s)	106, 138
PsuI	RGATCY	1 cut(s)	1030
RsaI	GTAC	3 cut(s)	729, 1157, 1270
RsaNI	GTAC	3 cut(s)	728, 1156, 1269
RseI	CAYNNNNRTG	2 cut(s)	165, 940
SaqAI	TTAA	9 cut(s)	41, 567, 747, 768, 1263, 1284, 1488, 1592, 1819
Sau3AI	GATC	4 cut(s)	1030, 1434, 1630, 1710
Sau96I	GGNCC	1 cut(s)	1525
ScrFI	CCNGG	2 cut(s)	1274, 1651
SduI	GDGCHC	2 cut(s)	339, 1474
SfaNI	GCATC	6 cut(s)	157, 179, 828, 856, 1135, 1630
SfoI	GGCGCC	1 cut(s)	178
Sfr274I	CTCGAG	1 cut(s)	798
SinI	GGWCC	1 cut(s)	1525
SlaI	CTCGAG	1 cut(s)	798
SmiMI	CAYNNNNRTG	2 cut(s)	165, 940
SmlI	CTYRAG	4 cut(s)	602, 798, 1283, 1591
SmoI	CTYRAG	4 cut(s)	602, 798, 1283, 1591
SspDI	GGCGCC	1 cut(s)	176
SspI	AATATT	1 cut(s)	1222
SspMI	CTAG	4 cut(s)	1028, 1097, 1430, 1541
StyD4I	CCNGG	2 cut(s)	1272, 1649
StyI	CCWWGG	1 cut(s)	1475
TaaI	ACNGT	7 cut(s)	341, 395, 435, 563, 572, 1268, 1357
TaiI	ACGT	5 cut(s)	128, 225, 312, 985, 1213
TaqI	TCGA	2 cut(s)	799, 1638
TatI	WGTACW	1 cut(s)	727
TfiI	GAWTC	2 cut(s)	1059, 1316
Tru1I	TTAA	9 cut(s)	41, 567, 747, 768, 1263, 1284, 1488, 1592, 1819
Tru9I	TTAA	9 cut(s)	41, 567, 747, 768, 1263, 1284, 1488, 1592, 1819
TscAI	CASTG	5 cut(s)	193, 334, 398, 1155, 1362
TseFI	GTSAC	5 cut(s)	188, 319, 395, 1479, 1579
Tsp45I	GTSAC	5 cut(s)	188, 319, 395, 1479, 1579
TspDTI	ATGAA	4 cut(s)	448, 1094, 1204, 1376
TspGWI	ACGGA	2 cut(s)	731, 1751
TspRI	CASTG	5 cut(s)	193, 334, 398, 1155, 1362
Vha464I	CTTAAG	2 cut(s)	1283, 1591
VneI	GTGCAC	1 cut(s)	1470
VpaK11BI	GGWCC	1 cut(s)	1525
XagI	CCTNNNNNAGG	1 cut(s)	1169
XapI	RAATTY	6 cut(s)	500, 722, 1133, 1178, 1243, 1450
XhoI	CTCGAG	1 cut(s)	798
XspI	CTAG	4 cut(s)	1028, 1097, 1430, 1541
Zsp2I	ATGCAT	1 cut(s)	172

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.