Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007689

Physical Location & Seq

Genomic Coordinates Forward (+)

24936176 .. 24939145

Gene Structure 2970 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1139800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1866 bp

ATGGCCTCTCCGCCCACCCTCCTCCTCTCCGCCGCGCTCCTACTAACCTCGGCCTGGTGCGTACTATCCCTCCCGCGCTGCCCAGACTGCGGCACCACCACCGTCCCTTACCCCCTCAGCACATCCCCCACGTGCGGCGACCAGTCGTACAAGATCCGCTGTTCCGCGGGCTCCCTCCTCTTCGACACCCTCAACAACTCCTACCCAATCGAATCCGTCTCCCCTGCCTCCCAGCGACTGGTCCTCCGCCCCTCCGCCTTCCTCCCCAACACCTGCATCGCAACCGATATAGTCCACGAGGGCCTCCAGCTCAACAACTCCCTCCCCTTCAACGTCACCAGCAGCAACACCATCCTCTACCTCAACTGCACCGACACCCTCCTCCGATCGCCGCTCAACTGCACGGCGTCGAGCCTCTGCCACACATACATCAACGGCACCGGATCGGTGGGCTCTTGCAAGGAATCGCTGTGCTGCACGTTTCGGGCGGGCGGGTCGTCGACGTCGTACAATATCCGGGTCAGAAACTCGGGTTGCAGCGCGTACACCAGTTTCGTGAACTTGGATCCGGGTTTGCCCGTTGACCGGTGGCCCGAACCGGGAGTGGAGCTACAGTGGACATCACCAAGGGAGCCCGAGTGTGGGACGCAGACGGATTGTGAGGGGAAGTCCACTTGTAGGTCCGACCCGGCTGTCGCTGGGGTCAGGAGGTGCTTTTGCGACTCTGGCTTTACGTGGGACCCGGTTCAAGGCTTATGCGTTGATGATAGCATTGGTTCAAAGGACCGCACCGGACTAATAGCAGGTTTAACTTGTGGGATTGGTGCAACACTAATCGCAGCCACCATAGCCTTTCTACTCTACAAGCGCCACAGGCGCATCAAAGAAGCACAAGCGCGCCTGACGAAAGAGCGCGAGGATATCCTAAATGCCAACGGAGGCAGAGCCGCTAAAGTCTTCACCGGGAATGAGATCAAAAGAGCAACAAACAGCTTCTCCAAAGACCGGCTCCTAGGCGCTGGTGGATACGGCGAAGTCTACCAAGGAACTCTTGTAGACGGCACTGTCGTGGCTGTCAAGATTGCTAAGATTGGAAACACCAAAGGCATTGACCAAGTTCTCAACGAGGTCCGAATTCTGTGCCAAGTTAACCACAAGAGCCTTGTCCACCTACTCGGCTGCTGCGTCGAGTTAGAGCAGCCTATTCTGGTCTACGAGTACATCGAGAATGGAACCCTTCTCGAACATCTGCAGGCTCGAAAACCTGGTGGCTGGAAACATCTTTCTTGGATGCAACGTCTCCAGATTGCGCTTGACACGGCCGAGGGTCTTGCTTATCTCCATTTCTCAGCTGTCCCTCCAATATATCACCGTGACGTGAAGTCTAGTAACATCCTGCTTGATGAGAAGCTGAATGCCAAGGTTTCGGATTTTGGGTTATCGCGGTTGGCTCACACGGATTTAAGTCACATATCAACGTGTGCTCAGGGAACCCTCGGATATCTTGATCCCGAATATTATAGGAACTATCAATTGACGGACAAGAGTGATGTTTATAGCTTCGGAGTTGTGCTCTTGGAGCTGCTGACATCTCAGAAAGCTATAGACTTCAACAGGGAAGCGGATGACGTGAACCTGGCAGTTTATGCGCAGAGGATGATGTTGGAGGAGAGGTTGATGGACGTGGTTGATCCGTTGCTGAAAGAGGGAGCGAATGCTTTGGAGCTGGACACGATGAAGGCGCTGGGGCTCCTTGCGTTGGGTTGCTTGGAGGAGCGTAGACAGAACCGGCCTTCGATGAAAGAAGTCACCGAGGAGATTGAGTACATTGCAAGCATTGCCACTGCTAAGGCTGTAGATGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

622

Amino Acids

67.74

Weight (kDa)

5.85

Isoelectric Point (pI)

38.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	27 - 81	1.6e-06	Wall-associated receptor kinase galacturonan-binding
Pkinase	PF00069	333 - 605	1.9e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	335 - 606	1.5e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007689)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02070
fragaria_vesca	FvH4_7g23160 FvH4_7g23250 FvH4_7g23370
malus_domestica	MD01G1139800.v1.1
prunus_persica	Prupe.2G240400_v2.0.a1 Prupe.6G261200_v2.0.a1
pyrus_communis	pycom01g16030 pycom08g15310
rosa_chinensis	RchiOBHm_Chr1g0368501 RchiOBHm_Chr3g0483591
rosa_laevigata	RLG00000027208
rosa_multiflora	Rmu_co8210558.1_g000001 Rmu_co8455625.1_g000001 Rmu_sc0006908.1_g000003 Rmu_sc0011394.1_g000011
rosa_roxburghii	Rroxscaffold_4G00288490
rosa_rugosa	Rorug01G0342300.1
rosa_samantha	Rh1AG349700 Rh1BG312700 Rh1BG312800 Rh1CG327600 Rh1DG344200 Rh1DG344300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	281
AasI	GACNNNNNNGTC	2 cut(s)	692, 1064
AatII	GACGTC	1 cut(s)	506
Acc16I	TGCGCA	1 cut(s)	1650
Acc36I	ACCTGC	2 cut(s)	281, 794
AccB1I	GGYRCC	2 cut(s)	92, 437
AccB7I	CCANNNNNTGG	1 cut(s)	238
AccBSI	CCGCTC	1 cut(s)	394
AccI	GTMKAC	5 cut(s)	500, 1038, 1056, 1212, 1780
AccII	CGCG	7 cut(s)	35, 76, 167, 542, 898, 915, 1444
AclWI	GGATC	6 cut(s)	148, 451, 560, 573, 1502, 1685
AcoI	YGGCCR	1 cut(s)	1320
AcsI	RAATTY	1 cut(s)	1134
AcvI	CACGTG	1 cut(s)	132
AcyI	GRCGYC	2 cut(s)	407, 503
AfaI	GTAC	6 cut(s)	63, 149, 509, 545, 1220, 1826
AflIII	ACRYGT	1 cut(s)	1478
AgeI	ACCGGT	1 cut(s)	585
AgsI	TTSAA	4 cut(s)	331, 749, 780, 1612
AhdI	GACNNNNNGTC	1 cut(s)	1381
AjiI	CACGTC	3 cut(s)	1378, 1630, 1684
AjnI	CCWGG	3 cut(s)	53, 1264, 1635
AleI	CACNNNNGTG	1 cut(s)	1067
AluBI	AGCT	9 cut(s)	310, 610, 993, 1352, 1411, 1560, 1582, 1601, 1726
AluI	AGCT	9 cut(s)	310, 610, 993, 1352, 1411, 1560, 1582, 1601, 1726
Alw21I	GWGCWC	2 cut(s)	1486, 1575
Alw26I	GTCTC	2 cut(s)	223, 1304
AlwI	GGATC	6 cut(s)	148, 451, 560, 573, 1502, 1685
AlwNI	CAGNNNCTG	1 cut(s)	238
Ama87I	CYCGRG	2 cut(s)	529, 635
AoxI	GGCC	6 cut(s)	3, 51, 301, 590, 1320, 1790
ApeKI	GCWGC	9 cut(s)	78, 342, 474, 537, 839, 1179, 1182, 1198, 1582
ApoI	RAATTY	1 cut(s)	1134
AsiGI	ACCGGT	1 cut(s)	585
AspA2I	CCTAGG	1 cut(s)	1012
AspS9I	GGNCC	7 cut(s)	241, 301, 591, 681, 739, 784, 1129
AsuC2I	CCSGG	6 cut(s)	518, 570, 600, 689, 743, 964
AsuHPI	GGTGA	5 cut(s)	328, 615, 952, 1361, 1801
AvaI	CYCGRG	2 cut(s)	529, 635
AvaII	GGWCC	5 cut(s)	241, 681, 739, 784, 1129
AvrII	CCTAGG	1 cut(s)	1012
BamHI	GGATCC	1 cut(s)	565
BanI	GGYRCC	2 cut(s)	92, 437
BanII	GRGCYC	4 cut(s)	173, 455, 636, 1752
BauI	CACGAG	1 cut(s)	296
BbrPI	CACGTG	1 cut(s)	132
BbsI	GAAGAC	1 cut(s)	949
Bbv12I	GWGCWC	2 cut(s)	1486, 1575
BbvCI	CCTCAGC	1 cut(s)	116
BbvI	GCAGC	9 cut(s)	65, 354, 461, 549, 851, 1166, 1169, 1210, 1569
BccI	CCATC	2 cut(s)	359, 1672
BceAI	ACGGC	5 cut(s)	420, 451, 1045, 1075, 1335
BcgI	CGANNNNNNTGC	4 cut(s)	1122, 1156, 1313, 1347
BciT130I	CCWGG	3 cut(s)	55, 1266, 1637
BciVI	GTATCC	1 cut(s)	1019
BcnI	CCSGG	6 cut(s)	518, 570, 600, 689, 743, 964
BcoDI	GTCTC	2 cut(s)	223, 1304
BfaI	CTAG	3 cut(s)	1013, 1386, 1864
BfmI	CTRYAG	4 cut(s)	611, 1250, 1602, 1854
BfoI	RGCGCY	3 cut(s)	871, 1020, 1745
BfuAI	ACCTGC	2 cut(s)	281, 794
BfuI	GTATCC	1 cut(s)	1019
BlnI	CCTAGG	1 cut(s)	1012
Bme1390I	CCNGG	9 cut(s)	55, 518, 570, 600, 689, 743, 964, 1266, 1637
Bme18I	GGWCC	5 cut(s)	241, 681, 739, 784, 1129
BmeRI	GACNNNNNGTC	1 cut(s)	1381
BmeT110I	CYCGRG	2 cut(s)	529, 635
BmgBI	CACGTC	3 cut(s)	1378, 1630, 1684
BmgT120I	GGNCC	7 cut(s)	241, 301, 591, 681, 739, 784, 1129
BmrFI	CCNGG	9 cut(s)	55, 518, 570, 600, 689, 743, 964, 1266, 1637
BmsI	GCATC	4 cut(s)	285, 888, 1281, 1849
BpiI	GAAGAC	1 cut(s)	949
BplI	GAGNNNNNCTC	2 cut(s)	1557, 1589
BpmI	CTGGAG	2 cut(s)	290, 1286
Bpu10I	CCTNAGC	3 cut(s)	116, 1485, 1848
BpuMI	CCSGG	6 cut(s)	518, 570, 600, 689, 743, 964
BsaAI	YACGTR	2 cut(s)	132, 735
BsaHI	GRCGYC	2 cut(s)	407, 503
BsaJI	CCNNGG	9 cut(s)	48, 165, 626, 1012, 1042, 1323, 1419, 1495, 1812
BsaWI	WCCGGW	3 cut(s)	440, 585, 791
Bse118I	RCCGGY	3 cut(s)	585, 1005, 1788
Bse1I	ACTGG	3 cut(s)	142, 243, 549
Bse3DI	GCAATG	2 cut(s)	1827, 1836
BseBI	CCWGG	3 cut(s)	55, 1266, 1637
BseDI	CCNNGG	9 cut(s)	48, 165, 626, 1012, 1042, 1323, 1419, 1495, 1812
BseGI	GGATG	6 cut(s)	122, 351, 1296, 1392, 1630, 1662
BseMI	GCAATG	2 cut(s)	1827, 1836
BseMII	CTCAG	4 cut(s)	130, 1362, 1499, 1607
BseNI	ACTGG	3 cut(s)	142, 243, 549
BsePI	GCGCGC	1 cut(s)	896
BseRI	GAGGAG	7 cut(s)	11, 14, 167, 371, 1682, 1787, 1829
BseX3I	CGGCCG	1 cut(s)	1320
BseXI	GCAGC	9 cut(s)	65, 354, 461, 549, 851, 1166, 1169, 1210, 1569
BseYI	CCCAGC	3 cut(s)	231, 698, 1744
BsgI	GTGCAG	3 cut(s)	352, 385, 460
Bsh1236I	CGCG	7 cut(s)	35, 76, 167, 542, 898, 915, 1444
Bsh1285I	CGRYCG	2 cut(s)	389, 1323
BshFI	GGCC	6 cut(s)	5, 53, 303, 592, 1322, 1792
BshNI	GGYRCC	2 cut(s)	92, 437
BshTI	ACCGGT	1 cut(s)	585
BsiEI	CGRYCG	2 cut(s)	389, 1323
BsiHKAI	GWGCWC	2 cut(s)	1486, 1575
BsiHKCI	CYCGRG	2 cut(s)	529, 635
BslFI	GGGAC	4 cut(s)	89, 658, 752, 1340
BsmAI	GTCTC	2 cut(s)	223, 1304
BsmBI	CGTCTC	2 cut(s)	223, 1304
BsmFI	GGGAC	4 cut(s)	89, 658, 752, 1340
BsmI	GAATGC	2 cut(s)	1420, 1720
BsnI	GGCC	6 cut(s)	5, 53, 303, 592, 1322, 1792
BsoBI	CYCGRG	2 cut(s)	529, 635
Bsp1286I	GDGCHC	6 cut(s)	173, 455, 636, 1486, 1575, 1752
Bsp143I	GATC	7 cut(s)	153, 386, 443, 565, 972, 1507, 1690
BspANI	GGCC	6 cut(s)	5, 53, 303, 592, 1322, 1792
BspCNI	CTCAG	4 cut(s)	129, 1361, 1498, 1606
BspFNI	CGCG	7 cut(s)	35, 76, 167, 542, 898, 915, 1444
BspMAI	CTGCAG	1 cut(s)	1254
BspMI	ACCTGC	2 cut(s)	281, 794
BspPI	GGATC	6 cut(s)	148, 451, 560, 573, 1502, 1685
BspT107I	GGYRCC	2 cut(s)	92, 437
BsrBI	CCGCTC	1 cut(s)	394
BsrDI	GCAATG	2 cut(s)	1827, 1836
BsrFI	RCCGGY	3 cut(s)	585, 1005, 1788
BsrI	ACTGG	3 cut(s)	142, 243, 549
BssAI	RCCGGY	3 cut(s)	585, 1005, 1788
BssECI	CCNNGG	9 cut(s)	48, 165, 626, 1012, 1042, 1323, 1419, 1495, 1812
BssHII	GCGCGC	1 cut(s)	896
BssMI	GATC	7 cut(s)	153, 386, 443, 565, 972, 1507, 1690
BssNI	GRCGYC	2 cut(s)	407, 503
BssSI	CACGAG	1 cut(s)	296
BssT1I	CCWWGG	4 cut(s)	626, 1012, 1042, 1419
Bst2BI	CACGAG	1 cut(s)	296
Bst2UI	CCWGG	3 cut(s)	55, 1266, 1637
Bst4CI	ACNGT	4 cut(s)	103, 615, 1066, 1373
Bst6I	CTCTTC	1 cut(s)	185
BstACI	GRCGYC	2 cut(s)	407, 503
BstAPI	GCANNNNNTGC	2 cut(s)	1646, 1838
BstBAI	YACGTR	2 cut(s)	132, 735
BstC8I	GCNNGC	5 cut(s)	169, 490, 898, 1254, 1834
BstDEI	CTNAG	6 cut(s)	116, 1086, 1348, 1485, 1593, 1848
BstDSI	CCRYGG	1 cut(s)	165
BstF5I	GGATG	6 cut(s)	122, 351, 1296, 1392, 1630, 1662
BstFNI	CGCG	7 cut(s)	35, 76, 167, 542, 898, 915, 1444
BstH2I	RGCGCY	3 cut(s)	871, 1020, 1745
BstKTI	GATC	7 cut(s)	156, 389, 446, 568, 975, 1510, 1693
BstMAI	GTCTC	2 cut(s)	223, 1304
BstMBI	GATC	7 cut(s)	153, 386, 443, 565, 972, 1507, 1690
BstMCI	CGRYCG	2 cut(s)	389, 1323
BstMWI	GCNNNNNNNGC	9 cut(s)	87, 726, 848, 874, 876, 1579, 1646, 1838, 1859
BstNI	CCWGG	3 cut(s)	55, 1266, 1637
BstSCI	CCNGG	9 cut(s)	53, 516, 568, 598, 687, 741, 962, 1264, 1635
BstSFI	CTRYAG	4 cut(s)	611, 1250, 1602, 1854
BstUI	CGCG	7 cut(s)	35, 76, 167, 542, 898, 915, 1444
BstV1I	GCAGC	9 cut(s)	65, 354, 461, 549, 851, 1166, 1169, 1210, 1569
BstV2I	GAAGAC	1 cut(s)	949
BstX2I	RGATCY	2 cut(s)	153, 565
BstYI	RGATCY	2 cut(s)	153, 565
BstZI	CGGCCG	1 cut(s)	1320
BsuI	GTATCC	1 cut(s)	1019
BsuRI	GGCC	6 cut(s)	5, 53, 303, 592, 1322, 1792
BtgI	CCRYGG	1 cut(s)	165
BtgZI	GCGATG	1 cut(s)	262
BtrI	CACGTC	3 cut(s)	1378, 1630, 1684
BtsCI	GGATG	6 cut(s)	122, 351, 1296, 1392, 1630, 1662
BtsI	GCAGTG	1 cut(s)	1842
BtsIMutI	CAGTG	3 cut(s)	620, 1062, 1842
BveI	ACCTGC	2 cut(s)	281, 794
Cac8I	GCNNGC	5 cut(s)	169, 490, 898, 1254, 1834
CaiI	CAGNNNCTG	1 cut(s)	238
Cfr10I	RCCGGY	3 cut(s)	585, 1005, 1788
Cfr13I	GGNCC	7 cut(s)	241, 301, 591, 681, 739, 784, 1129
Cfr42I	CCGCGG	1 cut(s)	168
CseI	GACGC	3 cut(s)	396, 655, 1174
CsiI	ACCWGGT	1 cut(s)	1264
Csp6I	GTAC	6 cut(s)	62, 148, 508, 544, 1219, 1825
CspAI	ACCGGT	1 cut(s)	585
CviQI	GTAC	6 cut(s)	62, 148, 508, 544, 1219, 1825
DdeI	CTNAG	6 cut(s)	116, 1086, 1348, 1485, 1593, 1848
DpnI	GATC	7 cut(s)	155, 388, 445, 567, 974, 1509, 1692
DpnII	GATC	7 cut(s)	153, 386, 443, 565, 972, 1507, 1690
DrdI	GACNNNNNNGTC	2 cut(s)	692, 1064
DriI	GACNNNNNGTC	1 cut(s)	1381
DseDI	GACNNNNNNGTC	2 cut(s)	692, 1064
EaeI	YGGCCR	1 cut(s)	1320
EagI	CGGCCG	1 cut(s)	1320
Eam1104I	CTCTTC	1 cut(s)	185
Eam1105I	GACNNNNNGTC	1 cut(s)	1381
EarI	CTCTTC	1 cut(s)	185
EciI	GGCGGA	3 cut(s)	19, 236, 244
EclXI	CGGCCG	1 cut(s)	1320
Eco130I	CCWWGG	4 cut(s)	626, 1012, 1042, 1419
Eco24I	GRGCYC	4 cut(s)	173, 455, 636, 1752
Eco32I	GATATC	2 cut(s)	922, 1502
Eco47I	GGWCC	5 cut(s)	241, 681, 739, 784, 1129
Eco52I	CGGCCG	1 cut(s)	1320
Eco72I	CACGTG	1 cut(s)	132
Eco88I	CYCGRG	2 cut(s)	529, 635
EcoO109I	RGGNCCY	2 cut(s)	301, 739
EcoRI	GAATTC	1 cut(s)	1134
EcoRII	CCWGG	3 cut(s)	53, 1264, 1635
EcoRV	GATATC	2 cut(s)	922, 1502
EcoT14I	CCWWGG	4 cut(s)	626, 1012, 1042, 1419
EcoT38I	GRGCYC	4 cut(s)	173, 455, 636, 1752
ErhI	CCWWGG	4 cut(s)	626, 1012, 1042, 1419
Esp3I	CGTCTC	2 cut(s)	223, 1304
FalI	AAGNNNNNCTT	2 cut(s)	1035, 1067
FaqI	GGGAC	4 cut(s)	89, 658, 752, 1340
FauI	CCCGC	4 cut(s)	81, 160, 481, 485
FblI	GTMKAC	5 cut(s)	500, 1038, 1056, 1212, 1780
FokI	GGATG	6 cut(s)	109, 338, 1303, 1379, 1637, 1669
FriOI	GRGCYC	4 cut(s)	173, 455, 636, 1752
FspBI	CTAG	3 cut(s)	1013, 1386, 1864
FspI	TGCGCA	1 cut(s)	1650
GsaI	CCCAGC	3 cut(s)	235, 702, 1748
GsuI	CTGGAG	2 cut(s)	290, 1286
HaeII	RGCGCY	3 cut(s)	871, 1020, 1745
HaeIII	GGCC	6 cut(s)	5, 53, 303, 592, 1322, 1792
HgaI	GACGC	3 cut(s)	396, 655, 1174
Hin1I	GRCGYC	2 cut(s)	407, 503
HincII	GTYRAC	3 cut(s)	501, 583, 1150
HindII	GTYRAC	3 cut(s)	501, 583, 1150
HinfI	GANTC	3 cut(s)	212, 464, 722
HpaI	GTTAAC	1 cut(s)	1150
HphI	GGTGA	5 cut(s)	328, 615, 952, 1361, 1801
Hpy188I	TCNGA	8 cut(s)	386, 524, 685, 1133, 1429, 1499, 1565, 1596
Hpy188III	TCNNGA	8 cut(s)	556, 706, 1078, 1225, 1241, 1303, 1505, 1511
Hpy99I	CGWCG	5 cut(s)	412, 502, 505, 508, 1190
HpyAV	CCTTC	5 cut(s)	268, 337, 1247, 1732, 1803
HpyCH4III	ACNGT	4 cut(s)	103, 615, 1066, 1373
HpyF10VI	GCNNNNNNNGC	9 cut(s)	87, 726, 848, 874, 876, 1579, 1646, 1838, 1859
HpyF3I	CTNAG	6 cut(s)	116, 1086, 1348, 1485, 1593, 1848
Hsp92I	GRCGYC	2 cut(s)	407, 503
KflI	GGGWCCC	1 cut(s)	739
KspAI	GTTAAC	1 cut(s)	1150
KspI	CCGCGG	1 cut(s)	168
Kzo9I	GATC	7 cut(s)	153, 386, 443, 565, 972, 1507, 1690
Lsp1109I	GCAGC	9 cut(s)	65, 354, 461, 549, 851, 1166, 1169, 1210, 1569
LweI	GCATC	4 cut(s)	285, 888, 1281, 1849
MabI	ACCWGGT	1 cut(s)	1264
MaeI	CTAG	3 cut(s)	1013, 1386, 1864
MaeIII	GTNAC	5 cut(s)	334, 1373, 1388, 1466, 1807
MalI	GATC	7 cut(s)	155, 388, 445, 567, 974, 1509, 1692
MbiI	CCGCTC	1 cut(s)	394
MboI	GATC	7 cut(s)	153, 386, 443, 565, 972, 1507, 1690
MboII	GAAGA	2 cut(s)	172, 949
MfeI	CAATTG	1 cut(s)	1532
MflI	RGATCY	2 cut(s)	153, 565
MhlI	GDGCHC	6 cut(s)	173, 455, 636, 1486, 1575, 1752
MluCI	AATT	2 cut(s)	1134, 1532
MlyI	GAGTC	1 cut(s)	716
MmeI	TCCRAC	2 cut(s)	708, 1644
MseI	TTAA	3 cut(s)	809, 1149, 1463
MslI	CAYNNNNRTG	1 cut(s)	1067
MspA1I	CMGCKG	3 cut(s)	159, 167, 1352
MspR9I	CCNGG	9 cut(s)	55, 518, 570, 600, 689, 743, 964, 1266, 1637
MunI	CAATTG	1 cut(s)	1532
Mva1269I	GAATGC	2 cut(s)	1420, 1720
MvaI	CCWGG	3 cut(s)	55, 1266, 1637
MvnI	CGCG	7 cut(s)	35, 76, 167, 542, 898, 915, 1444
MwoI	GCNNNNNNNGC	9 cut(s)	87, 726, 848, 874, 876, 1579, 1646, 1838, 1859
NciI	CCSGG	6 cut(s)	518, 570, 600, 689, 743, 964
NdeII	GATC	7 cut(s)	153, 386, 443, 565, 972, 1507, 1690
NmeAIII	GCCGAG	3 cut(s)	29, 1155, 1348
NmuCI	GTSAC	4 cut(s)	334, 1373, 1466, 1807
NsbI	TGCGCA	1 cut(s)	1650
OliI	CACNNNNGTG	1 cut(s)	1067
PaqCI	CACCTGC	1 cut(s)	281
PauI	GCGCGC	1 cut(s)	896
PcsI	WCGNNNNNNNCGW	4 cut(s)	503, 1065, 1182, 1221
PctI	GAATGC	2 cut(s)	1420, 1720
PfeI	GAWTC	2 cut(s)	212, 464
PflMI	CCANNNNNTGG	1 cut(s)	238
PinAI	ACCGGT	1 cut(s)	585
Ple19I	CGATCG	1 cut(s)	389
PleI	GAGTC	1 cut(s)	716
PmaCI	CACGTG	1 cut(s)	132
PmlI	CACGTG	1 cut(s)	132
PpsI	GAGTC	1 cut(s)	716
Ppu21I	YACGTR	2 cut(s)	132, 735
PpuMI	RGGWCCY	1 cut(s)	739
Psp5II	RGGWCCY	1 cut(s)	739
Psp6I	CCWGG	3 cut(s)	53, 1264, 1635
PspCI	CACGTG	1 cut(s)	132
PspFI	CCCAGC	3 cut(s)	231, 698, 1744
PspGI	CCWGG	3 cut(s)	53, 1264, 1635
PspPI	GGNCC	7 cut(s)	241, 301, 591, 681, 739, 784, 1129
PspPPI	RGGWCCY	1 cut(s)	739
PstI	CTGCAG	1 cut(s)	1254
PstNI	CAGNNNCTG	1 cut(s)	238
PsuI	RGATCY	2 cut(s)	153, 565
PteI	GCGCGC	1 cut(s)	896
PvuI	CGATCG	1 cut(s)	389
PvuII	CAGCTG	1 cut(s)	1352
RsaI	GTAC	6 cut(s)	63, 149, 509, 545, 1220, 1826
RsaNI	GTAC	6 cut(s)	62, 148, 508, 544, 1219, 1825
RseI	CAYNNNNRTG	1 cut(s)	1067
SacII	CCGCGG	1 cut(s)	168
SalI	GTCGAC	1 cut(s)	499
SaqAI	TTAA	3 cut(s)	809, 1149, 1463
Sau3AI	GATC	7 cut(s)	153, 386, 443, 565, 972, 1507, 1690
Sau96I	GGNCC	7 cut(s)	241, 301, 591, 681, 739, 784, 1129
SchI	GAGTC	1 cut(s)	716
ScrFI	CCNGG	9 cut(s)	55, 518, 570, 600, 689, 743, 964, 1266, 1637
SduI	GDGCHC	6 cut(s)	173, 455, 636, 1486, 1575, 1752
SexAI	ACCWGGT	1 cut(s)	1264
SfaNI	GCATC	4 cut(s)	285, 888, 1281, 1849
SfcI	CTRYAG	4 cut(s)	611, 1250, 1602, 1854
Sfr303I	CCGCGG	1 cut(s)	168
SgrBI	CCGCGG	1 cut(s)	168
SgrDI	CGTCGACG	1 cut(s)	499
SinI	GGWCC	5 cut(s)	241, 681, 739, 784, 1129
SmiMI	CAYNNNNRTG	1 cut(s)	1067
Sse9I	AATT	2 cut(s)	1134, 1532
SspI	AATATT	1 cut(s)	1517
SspMI	CTAG	3 cut(s)	1013, 1386, 1864
StyD4I	CCNGG	9 cut(s)	53, 516, 568, 598, 687, 741, 962, 1264, 1635
StyI	CCWWGG	4 cut(s)	626, 1012, 1042, 1419
TaaI	ACNGT	4 cut(s)	103, 615, 1066, 1373
TaqI	TCGA	9 cut(s)	183, 210, 410, 500, 1188, 1224, 1242, 1258, 1796
TasI	AATT	2 cut(s)	1134, 1532
TatI	WGTACW	2 cut(s)	1218, 1824
TauI	GCSGC	5 cut(s)	35, 93, 138, 394, 950
TfiI	GAWTC	2 cut(s)	212, 464
Tru1I	TTAA	3 cut(s)	809, 1149, 1463
Tru9I	TTAA	3 cut(s)	809, 1149, 1463
TscAI	CASTG	3 cut(s)	620, 1069, 1849
TseFI	GTSAC	4 cut(s)	334, 1373, 1466, 1807
TseI	GCWGC	9 cut(s)	78, 342, 474, 537, 839, 1179, 1182, 1198, 1582
Tsp45I	GTSAC	4 cut(s)	334, 1373, 1466, 1807
TspDTI	ATGAA	2 cut(s)	1751, 1814
TspGWI	ACGGA	6 cut(s)	205, 668, 951, 1472, 1553, 1683
TspRI	CASTG	3 cut(s)	620, 1069, 1849
Van91I	CCANNNNNTGG	1 cut(s)	238
VpaK11BI	GGWCC	5 cut(s)	241, 681, 739, 784, 1129
XapI	RAATTY	1 cut(s)	1134
XmaJI	CCTAGG	1 cut(s)	1012
XmiI	GTMKAC	5 cut(s)	500, 1038, 1056, 1212, 1780
XspI	CTAG	3 cut(s)	1013, 1386, 1864
ZraI	GACGTC	1 cut(s)	504

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.