Rh1DG344200

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0007689
Physical Location & Seq
Forward (+)
56826908 .. 56827270
363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG344200.1

Sequence Viewer

Length: 363 bp
ATGGCCGTTACTCCCAACCTCCGCCTCTTAACGGCGTCGCTGCTGCTCCTTTCGTGCGCGTGGACCTCACTCTCCGTCCAGCTCTGCCCACCCTGCGGCAACACCACCGTCCCCTACCCTTTCAGCACCTCCCCCACCTGCGGAGACCAGTCATACAAGATCCGCTGCGACGCGGCAGGCTCCCTCCTCTTCGACACCCTCAACAACTCCTACCCGATCGCCTCCATCTCCCCCTCCGCCCAACGACTCGTCGTACGCCCCTCCAGCTTCGTGTCGTCCAACTCCTGCGTCACGTCGGATATCACCCACCAGGGAATCCAGCTCAACGAGTCCCTCCCCTTCAACGTCACCAGTAGGCAGTAG

Protein Analysis

120

Amino Acids

12.84

Weight (kDa)

7.58

Isoelectric Point (pI)

47.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 29 - 85 1.1e-10 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 146
AccII CGCG 2 cut(s) 59, 173
AciI CCGC 6 cut(s) 22, 96, 141, 163, 173, 237
AclWI GGATC 1 cut(s) 154
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 35
AfaI GTAC 1 cut(s) 255
AfiI CCNNNNNNNGG 3 cut(s) 31, 95, 140
AgsI TTSAA 1 cut(s) 343
AjiI CACGTC 1 cut(s) 294
AjnI CCWGG 1 cut(s) 309
AluBI AGCT 3 cut(s) 82, 267, 322
AluI AGCT 3 cut(s) 82, 267, 322
Alw26I GTCTC 1 cut(s) 138
AlwI GGATC 1 cut(s) 154
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 40, 43, 165
AspLEI GCGC 1 cut(s) 59
AspS9I GGNCC 1 cut(s) 63
AsuHPI GGTGA 2 cut(s) 295, 340
AvaII GGWCC 1 cut(s) 63
BbvI GCAGC 3 cut(s) 27, 30, 152
BccI CCATC 1 cut(s) 233
BceAI ACGGC 1 cut(s) 48
BciT130I CCWGG 1 cut(s) 311
BcoDI GTCTC 1 cut(s) 138
BfuAI ACCTGC 1 cut(s) 146
BisI GCNGC 5 cut(s) 41, 44, 97, 166, 174
BlsI GCNGC 5 cut(s) 42, 45, 98, 167, 175
Bme1390I CCNGG 1 cut(s) 311
Bme18I GGWCC 1 cut(s) 63
BmgBI CACGTC 1 cut(s) 294
BmgT120I GGNCC 1 cut(s) 63
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 1 cut(s) 311
BpmI CTGGAG 1 cut(s) 247
BsaHI GRCGYC 1 cut(s) 35
BsaI GGTCTC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 310
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 95, 140
Bse1I ACTGG 2 cut(s) 148, 351
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 1 cut(s) 310
BseLI CCNNNNNNNGG 3 cut(s) 31, 95, 140
BseNI ACTGG 2 cut(s) 148, 351
BseRI GAGGAG 1 cut(s) 176
BseXI GCAGC 3 cut(s) 27, 30, 152
Bsh1236I CGCG 2 cut(s) 59, 173
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 219
BsiWI CGTACG 1 cut(s) 253
BslFI GGGAC 2 cut(s) 95, 316
BslI CCNNNNNNNGG 3 cut(s) 31, 95, 140
BsmAI GTCTC 1 cut(s) 138
BsmFI GGGAC 2 cut(s) 95, 316
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 138
Bsp143I GATC 2 cut(s) 159, 216
BspACI CCGC 6 cut(s) 22, 96, 141, 163, 173, 237
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 2 cut(s) 59, 173
BspLI GGNNCC 1 cut(s) 181
BspMI ACCTGC 1 cut(s) 146
BspPI GGATC 1 cut(s) 154
BspTNI GGTCTC 1 cut(s) 138
BsrI ACTGG 2 cut(s) 148, 351
BssECI CCNNGG 1 cut(s) 310
BssMI GATC 2 cut(s) 159, 216
BssNI GRCGYC 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 1 cut(s) 109
Bst6I CTCTTC 1 cut(s) 194
BstACI GRCGYC 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 178
BstFNI CGCG 2 cut(s) 59, 173
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 2 cut(s) 162, 219
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 2 cut(s) 159, 216
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 2 cut(s) 93, 264
BstNI CCWGG 1 cut(s) 311
BstSCI CCNGG 1 cut(s) 309
BstUI CGCG 2 cut(s) 59, 173
BstV1I GCAGC 3 cut(s) 27, 30, 152
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
BsuRI GGCC 1 cut(s) 5
BtrI CACGTC 1 cut(s) 294
BveI ACCTGC 1 cut(s) 146
Cac8I GCNNGC 1 cut(s) 178
CfoI GCGC 1 cut(s) 59
Cfr13I GGNCC 1 cut(s) 63
CseI GACGC 3 cut(s) 24, 179, 277
Csp6I GTAC 1 cut(s) 254
CviJI RGCY 5 cut(s) 5, 82, 180, 267, 322
CviKI_1 RGCY 5 cut(s) 5, 82, 180, 267, 322
CviQI GTAC 1 cut(s) 254
DpnI GATC 2 cut(s) 161, 218
DpnII GATC 2 cut(s) 159, 216
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
EciI GGCGGA 2 cut(s) 11, 226
Eco31I GGTCTC 1 cut(s) 138
Eco32I GATATC 1 cut(s) 301
Eco47I GGWCC 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 309
EcoRV GATATC 1 cut(s) 301
FaiI YATR 1 cut(s) 154
FaqI GGGAC 2 cut(s) 95, 316
Fnu4HI GCNGC 5 cut(s) 41, 44, 97, 166, 174
Fsp4HI GCNGC 5 cut(s) 41, 44, 97, 166, 174
GlaI GCGC 1 cut(s) 58
GluI GCNGC 5 cut(s) 41, 44, 97, 166, 174
GsuI CTGGAG 1 cut(s) 247
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 3 cut(s) 24, 179, 277
HhaI GCGC 1 cut(s) 59
Hin1I GRCGYC 1 cut(s) 35
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HinfI GANTC 3 cut(s) 246, 315, 329
HphI GGTGA 2 cut(s) 295, 340
Hpy166II GTNNAC 1 cut(s) 63
Hpy188I TCNGA 1 cut(s) 298
Hpy8I GTNNAC 1 cut(s) 63
Hpy99I CGWCG 4 cut(s) 40, 173, 254, 298
HpyAV CCTTC 1 cut(s) 349
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4IV ACGT 2 cut(s) 293, 345
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 264
HpySE526I ACGT 2 cut(s) 293, 345
Hsp92I GRCGYC 1 cut(s) 35
HspAI GCGC 1 cut(s) 57
Kzo9I GATC 2 cut(s) 159, 216
LmnI GCTCC 2 cut(s) 51, 185
Lsp1109I GCAGC 3 cut(s) 27, 30, 152
MaeII ACGT 2 cut(s) 293, 345
MaeIII GTNAC 3 cut(s) 7, 289, 346
MalI GATC 2 cut(s) 161, 218
MboI GATC 2 cut(s) 159, 216
MboII GAAGA 1 cut(s) 181
MflI RGATCY 1 cut(s) 159
MlyI GAGTC 2 cut(s) 240, 338
MmeI TCCRAC 2 cut(s) 276, 303
MseI TTAA 1 cut(s) 29
MspA1I CMGCKG 1 cut(s) 165
MspR9I CCNGG 1 cut(s) 311
MvaI CCWGG 1 cut(s) 311
MvnI CGCG 2 cut(s) 59, 173
MwoI GCNNNNNNNGC 2 cut(s) 93, 264
NdeII GATC 2 cut(s) 159, 216
NlaIV GGNNCC 1 cut(s) 181
NmuCI GTSAC 2 cut(s) 289, 346
PaqCI CACCTGC 1 cut(s) 146
PfeI GAWTC 1 cut(s) 315
Pfl23II CGTACG 1 cut(s) 253
PkrI GCNGC 5 cut(s) 42, 45, 98, 167, 175
Ple19I CGATCG 1 cut(s) 219
PleI GAGTC 2 cut(s) 240, 337
PpsI GAGTC 2 cut(s) 240, 337
Psp6I CCWGG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 309
PspLI CGTACG 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 181
PspPI GGNCC 1 cut(s) 63
PsuI RGATCY 1 cut(s) 159
PvuI CGATCG 1 cut(s) 219
RsaI GTAC 1 cut(s) 255
RsaNI GTAC 1 cut(s) 254
SaqAI TTAA 1 cut(s) 29
SatI GCNGC 5 cut(s) 41, 44, 97, 166, 174
Sau3AI GATC 2 cut(s) 159, 216
Sau96I GGNCC 1 cut(s) 63
SchI GAGTC 2 cut(s) 240, 338
ScrFI CCNGG 1 cut(s) 311
SetI ASST 9 cut(s) 21, 68, 84, 131, 140, 269, 296, 324, 348
SinI GGWCC 1 cut(s) 63
SsiI CCGC 6 cut(s) 22, 96, 141, 163, 173, 237
StyD4I CCNGG 1 cut(s) 309
TaaI ACNGT 1 cut(s) 109
TaiI ACGT 2 cut(s) 296, 348
TaqI TCGA 1 cut(s) 192
TauI GCSGC 2 cut(s) 99, 176
TfiI GAWTC 1 cut(s) 315
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TseFI GTSAC 2 cut(s) 289, 346
TseI GCWGC 3 cut(s) 40, 43, 165
Tsp45I GTSAC 2 cut(s) 289, 346
TspGWI ACGGA 1 cut(s) 64
VpaK11BI GGWCC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.