RchiOBHm_Chr3g0483591

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007689
Physical Location & Seq
Reverse (-)
29873251 .. 29874131
881 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44834

Sequence Viewer

Length: 855 bp
ATGTCCGTTTCTCCCAACCTCCGCCTTTTAACGGCGTCGCTGCTGCTCCTAACGTGCGCGTGGACCTCACTCTCCGTCCAGCTCTGCCCACCCTGCGGCAACACCACCGTCCCCTACCCTCTCAGCACCTCCCCCACCTGCGGGGACCAGTCATACAAGATCCGCTGCGACGCGGCGGCAGGCTCCCTCCTCTTCGACACCCTCAACAACTCCTACCCCATCGCCTCCATCTCCCCCTCCGCCCAACGACTCGTCGTACGCCCCTCCAGCTTTGTGTCGTCCAACTCCTGCGTCACGTCGGATATCACCCACCAGGGAATCCAGCTCAACGAGTCCCTCCCCTTCAACGTCACCAGTAGCAATACCATCTTGTACCTCAACTGTACGAACACTCTTCTCCGGTCGCCGTTGAACTGCACGTCGTCGAGTCTCTGCCACACGTATGCGAACAACACGAATGCGGTCGGGTCGTGCCAGAAGGCTCCGATTTGCTGTACATTTCGGGCGGGCGGGTCGTCGACGTCGTATGTGATTCGGGTCAGGGACTCGGGTTGTAGTGCTTATACGAGTTTTGTGAATTTGGATCCGGTTCTTCCGGTGAACCGGTGGCCCGAACCGGGAGTGGAGATACAATGGGTGGGGCCGAGGGAGCCGCTGTGTAGTGCGCAGTCGGATTGTGACGGGAAGTCGGTGTGTGGATCCGACCCGGTTCAAAATGGGGTGCGGAGGTGCATCTGTAACTCCGGGTTTACGTGGGACCCGGTTTCTGGGTTTTGCGTTGATGGTGAGTTTTTTTGTTTGGTTTATGTTATTGGAACTAGCATTCCTCCACACATGCTCTGCATGTGCAAGTAA

Protein Analysis

284

Amino Acids

30.4

Weight (kDa)

6.75

Isoelectric Point (pI)

49.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 29 - 86 3.9e-08 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 146
AatII GACGTC 1 cut(s) 524
Acc16I TGCGCA 1 cut(s) 666
Acc36I ACCTGC 1 cut(s) 146
AccI GTMKAC 1 cut(s) 518
AccII CGCG 2 cut(s) 59, 173
AclWI GGATC 5 cut(s) 154, 578, 591, 693, 706
AcsI RAATTY 1 cut(s) 577
AcyI GRCGYC 2 cut(s) 35, 521
AfaI GTAC 4 cut(s) 258, 374, 385, 496
AfiI CCNNNNNNNGG 6 cut(s) 31, 95, 140, 141, 617, 767
AflIII ACRYGT 1 cut(s) 438
AgeI ACCGGT 1 cut(s) 603
AgsI TTSAA 3 cut(s) 346, 412, 713
AhdI GACNNNNNGTC 1 cut(s) 685
AjiI CACGTC 2 cut(s) 297, 420
AjnI CCWGG 1 cut(s) 312
AloI GAACNNNNNNTCC 2 cut(s) 808, 840
AluBI AGCT 3 cut(s) 82, 270, 325
AluI AGCT 3 cut(s) 82, 270, 325
Alw26I GTCTC 1 cut(s) 434
AlwI GGATC 5 cut(s) 154, 578, 591, 693, 706
Ama87I CYCGRG 1 cut(s) 547
AoxI GGCC 2 cut(s) 608, 641
ApeKI GCWGC 3 cut(s) 40, 43, 165
ApoI RAATTY 1 cut(s) 577
AsiGI ACCGGT 1 cut(s) 603
AspLEI GCGC 2 cut(s) 59, 667
AspS9I GGNCC 5 cut(s) 63, 145, 609, 641, 757
AsuC2I CCSGG 4 cut(s) 618, 707, 745, 761
AsuHPI GGTGA 4 cut(s) 298, 343, 610, 797
AvaI CYCGRG 1 cut(s) 547
AvaII GGWCC 3 cut(s) 63, 145, 757
BamHI GGATCC 2 cut(s) 583, 698
BbvI GCAGC 3 cut(s) 27, 30, 152
BccI CCATC 4 cut(s) 227, 236, 374, 776
BceAI ACGGC 2 cut(s) 48, 391
BciT130I CCWGG 1 cut(s) 314
BcnI CCSGG 4 cut(s) 618, 707, 745, 761
BcoDI GTCTC 1 cut(s) 434
BfaI CTAG 1 cut(s) 819
BfuAI ACCTGC 1 cut(s) 146
BisI GCNGC 7 cut(s) 41, 44, 97, 166, 174, 177, 653
BlsI GCNGC 7 cut(s) 42, 45, 98, 167, 175, 178, 654
Bme1390I CCNGG 5 cut(s) 314, 618, 707, 745, 761
Bme18I GGWCC 3 cut(s) 63, 145, 757
BmeRI GACNNNNNGTC 1 cut(s) 685
BmeT110I CYCGRG 1 cut(s) 547
BmgBI CACGTC 2 cut(s) 297, 420
BmgT120I GGNCC 5 cut(s) 63, 145, 609, 641, 757
BmiI GGNNCC 9 cut(s) 146, 184, 483, 585, 642, 651, 700, 758, 759
BmrFI CCNGG 5 cut(s) 314, 618, 707, 745, 761
BmsI GCATC 1 cut(s) 741
BpmI CTGGAG 1 cut(s) 250
BpuMI CCSGG 4 cut(s) 618, 707, 745, 761
BsaAI YACGTR 2 cut(s) 441, 753
BsaHI GRCGYC 2 cut(s) 35, 521
BsaJI CCNNGG 2 cut(s) 313, 644
BsaWI WCCGGW 4 cut(s) 399, 586, 595, 603
BsaXI ACNNNNNCTCC 2 cut(s) 641, 671
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 95, 140, 141, 617, 767
Bse118I RCCGGY 1 cut(s) 603
Bse1I ACTGG 2 cut(s) 148, 354
BseBI CCWGG 1 cut(s) 314
BseDI CCNNGG 2 cut(s) 313, 644
BseLI CCNNNNNNNGG 6 cut(s) 31, 95, 140, 141, 617, 767
BseMII CTCAG 1 cut(s) 136
BseNI ACTGG 2 cut(s) 148, 354
BseRI GAGGAG 1 cut(s) 179
BseXI GCAGC 3 cut(s) 27, 30, 152
BsgI GTGCAG 1 cut(s) 400
Bsh1236I CGCG 2 cut(s) 59, 173
Bsh1285I CGRYCG 2 cut(s) 404, 465
BshFI GGCC 2 cut(s) 610, 643
BshTI ACCGGT 1 cut(s) 603
BsiEI CGRYCG 2 cut(s) 404, 465
BsiHKCI CYCGRG 1 cut(s) 547
BsiSI CCGG 8 cut(s) 400, 587, 596, 604, 617, 707, 744, 761
BsiWI CGTACG 1 cut(s) 256
BslFI GGGAC 5 cut(s) 95, 158, 319, 557, 770
BslI CCNNNNNNNGG 6 cut(s) 31, 95, 140, 141, 617, 767
BsmAI GTCTC 1 cut(s) 434
BsmFI GGGAC 5 cut(s) 95, 158, 319, 557, 770
BsmI GAATGC 2 cut(s) 463, 822
BsnI GGCC 2 cut(s) 610, 643
BsoBI CYCGRG 1 cut(s) 547
Bsp1407I TGTACA 1 cut(s) 494
Bsp143I GATC 3 cut(s) 159, 583, 698
BspANI GGCC 2 cut(s) 610, 643
BspCNI CTCAG 1 cut(s) 135
BspFNI CGCG 2 cut(s) 59, 173
BspLI GGNNCC 9 cut(s) 146, 184, 483, 585, 642, 651, 700, 758, 759
BspMI ACCTGC 1 cut(s) 146
BspPI GGATC 5 cut(s) 154, 578, 591, 693, 706
BsrFI RCCGGY 1 cut(s) 603
BsrGI TGTACA 1 cut(s) 494
BsrI ACTGG 2 cut(s) 148, 354
BssAI RCCGGY 1 cut(s) 603
BssECI CCNNGG 2 cut(s) 313, 644
BssMI GATC 3 cut(s) 159, 583, 698
BssNI GRCGYC 2 cut(s) 35, 521
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 2 cut(s) 109, 383
Bst6I CTCTTC 2 cut(s) 197, 399
BstACI GRCGYC 2 cut(s) 35, 521
BstAUI TGTACA 1 cut(s) 494
BstBAI YACGTR 2 cut(s) 441, 753
BstC8I GCNNGC 2 cut(s) 181, 508
BstDEI CTNAG 1 cut(s) 122
BstFNI CGCG 2 cut(s) 59, 173
BstHHI GCGC 2 cut(s) 59, 667
BstKTI GATC 3 cut(s) 162, 586, 701
BstMAI GTCTC 1 cut(s) 434
BstMBI GATC 3 cut(s) 159, 583, 698
BstMCI CGRYCG 2 cut(s) 404, 465
BstMWI GCNNNNNNNGC 3 cut(s) 93, 267, 649
BstNI CCWGG 1 cut(s) 314
BstNSI RCATGY 2 cut(s) 838, 847
BstSCI CCNGG 5 cut(s) 312, 616, 705, 743, 759
BstUI CGCG 2 cut(s) 59, 173
BstV1I GCAGC 3 cut(s) 27, 30, 152
BstX2I RGATCY 3 cut(s) 159, 583, 698
BstYI RGATCY 3 cut(s) 159, 583, 698
BsuRI GGCC 2 cut(s) 610, 643
BtgZI GCGATG 1 cut(s) 205
BtrI CACGTC 2 cut(s) 297, 420
BveI ACCTGC 1 cut(s) 146
Cac8I GCNNGC 2 cut(s) 181, 508
CfoI GCGC 2 cut(s) 59, 667
Cfr10I RCCGGY 1 cut(s) 603
Cfr13I GGNCC 5 cut(s) 63, 145, 609, 641, 757
CseI GACGC 3 cut(s) 24, 179, 280
Csp6I GTAC 4 cut(s) 257, 373, 384, 495
CspAI ACCGGT 1 cut(s) 603
CviAII CATG 2 cut(s) 835, 844
CviJI RGCY 8 cut(s) 82, 183, 270, 325, 482, 610, 643, 652
CviKI_1 RGCY 8 cut(s) 82, 183, 270, 325, 482, 610, 643, 652
CviQI GTAC 4 cut(s) 257, 373, 384, 495
DdeI CTNAG 1 cut(s) 122
DpnI GATC 3 cut(s) 161, 585, 700
DpnII GATC 3 cut(s) 159, 583, 698
DriI GACNNNNNGTC 1 cut(s) 685
Eam1104I CTCTTC 2 cut(s) 197, 399
Eam1105I GACNNNNNGTC 1 cut(s) 685
EarI CTCTTC 2 cut(s) 197, 399
EciI GGCGGA 2 cut(s) 11, 229
Eco32I GATATC 1 cut(s) 304
Eco47I GGWCC 3 cut(s) 63, 145, 757
Eco88I CYCGRG 1 cut(s) 547
EcoO109I RGGNCCY 1 cut(s) 757
EcoRII CCWGG 1 cut(s) 312
EcoRV GATATC 1 cut(s) 304
FaeI CATG 2 cut(s) 838, 847
FaiI YATR 7 cut(s) 154, 444, 528, 564, 807, 836, 845
FaqI GGGAC 5 cut(s) 95, 158, 319, 557, 770
FatI CATG 2 cut(s) 834, 843
FauI CCCGC 3 cut(s) 134, 499, 503
FblI GTMKAC 1 cut(s) 518
Fnu4HI GCNGC 7 cut(s) 41, 44, 97, 166, 174, 177, 653
Fsp4HI GCNGC 7 cut(s) 41, 44, 97, 166, 174, 177, 653
FspBI CTAG 1 cut(s) 819
FspI TGCGCA 1 cut(s) 666
GlaI GCGC 2 cut(s) 58, 666
GluI GCNGC 7 cut(s) 41, 44, 97, 166, 174, 177, 653
GsuI CTGGAG 1 cut(s) 250
HaeIII GGCC 2 cut(s) 610, 643
HapII CCGG 8 cut(s) 400, 587, 596, 604, 617, 707, 744, 761
HgaI GACGC 3 cut(s) 24, 179, 280
HhaI GCGC 2 cut(s) 59, 667
Hin1I GRCGYC 2 cut(s) 35, 521
Hin1II CATG 2 cut(s) 838, 847
Hin6I GCGC 2 cut(s) 57, 665
HinP1I GCGC 2 cut(s) 57, 665
HincII GTYRAC 1 cut(s) 519
HindII GTYRAC 1 cut(s) 519
HinfI GANTC 6 cut(s) 249, 318, 332, 427, 532, 545
HpaII CCGG 8 cut(s) 400, 587, 596, 604, 617, 707, 744, 761
HphI GGTGA 4 cut(s) 298, 343, 610, 797
Hpy166II GTNNAC 4 cut(s) 63, 519, 601, 750
Hpy188I TCNGA 4 cut(s) 301, 486, 673, 703
Hpy8I GTNNAC 4 cut(s) 63, 519, 601, 750
Hpy99I CGWCG 9 cut(s) 40, 173, 257, 301, 424, 427, 520, 523, 526
HpyAV CCTTC 2 cut(s) 352, 472
HpyCH4III ACNGT 2 cut(s) 109, 383
HpyCH4IV ACGT 7 cut(s) 53, 296, 348, 419, 440, 521, 752
HpyCH4V TGCA 4 cut(s) 417, 732, 843, 849
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 267, 649
HpyF3I CTNAG 1 cut(s) 122
HpySE526I ACGT 7 cut(s) 53, 296, 348, 419, 440, 521, 752
Hsp92I GRCGYC 2 cut(s) 35, 521
Hsp92II CATG 2 cut(s) 838, 847
HspAI GCGC 2 cut(s) 57, 665
KflI GGGWCCC 1 cut(s) 757
Kzo9I GATC 3 cut(s) 159, 583, 698
LmnI GCTCC 4 cut(s) 51, 188, 487, 649
Lsp1109I GCAGC 3 cut(s) 27, 30, 152
LweI GCATC 1 cut(s) 741
MaeI CTAG 1 cut(s) 819
MaeII ACGT 7 cut(s) 53, 296, 348, 419, 440, 521, 752
MaeIII GTNAC 4 cut(s) 292, 349, 677, 737
MalI GATC 3 cut(s) 161, 585, 700
MboI GATC 3 cut(s) 159, 583, 698
MboII GAAGA 3 cut(s) 184, 386, 584
MflI RGATCY 3 cut(s) 159, 583, 698
MluCI AATT 1 cut(s) 577
MlyI GAGTC 4 cut(s) 243, 341, 436, 539
MmeI TCCRAC 4 cut(s) 279, 306, 651, 726
MseI TTAA 1 cut(s) 29
MslI CAYNNNNRTG 1 cut(s) 441
MspA1I CMGCKG 2 cut(s) 165, 655
MspI CCGG 8 cut(s) 400, 587, 596, 604, 617, 707, 744, 761
MspR9I CCNGG 5 cut(s) 314, 618, 707, 745, 761
Mva1269I GAATGC 2 cut(s) 463, 822
MvaI CCWGG 1 cut(s) 314
MvnI CGCG 2 cut(s) 59, 173
MwoI GCNNNNNNNGC 3 cut(s) 93, 267, 649
NciI CCSGG 4 cut(s) 618, 707, 745, 761
NdeII GATC 3 cut(s) 159, 583, 698
NlaIII CATG 2 cut(s) 838, 847
NlaIV GGNNCC 9 cut(s) 146, 184, 483, 585, 642, 651, 700, 758, 759
NmeAIII GCCGAG 1 cut(s) 669
NmuCI GTSAC 3 cut(s) 292, 349, 677
NsbI TGCGCA 1 cut(s) 666
NspI RCATGY 2 cut(s) 838, 847
PaqCI CACCTGC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 521
PctI GAATGC 2 cut(s) 463, 822
PfeI GAWTC 2 cut(s) 318, 532
Pfl23II CGTACG 1 cut(s) 256
PinAI ACCGGT 1 cut(s) 603
PkrI GCNGC 7 cut(s) 42, 45, 98, 167, 175, 178, 654
PleI GAGTC 4 cut(s) 243, 340, 435, 539
PpsI GAGTC 4 cut(s) 243, 340, 435, 539
Ppu21I YACGTR 2 cut(s) 441, 753
PpuMI RGGWCCY 1 cut(s) 757
Psp5II RGGWCCY 1 cut(s) 757
Psp6I CCWGG 1 cut(s) 312
PspGI CCWGG 1 cut(s) 312
PspLI CGTACG 1 cut(s) 256
PspN4I GGNNCC 9 cut(s) 146, 184, 483, 585, 642, 651, 700, 758, 759
PspPI GGNCC 5 cut(s) 63, 145, 609, 641, 757
PspPPI RGGWCCY 1 cut(s) 757
PsuI RGATCY 3 cut(s) 159, 583, 698
RsaI GTAC 4 cut(s) 258, 374, 385, 496
RsaNI GTAC 4 cut(s) 257, 373, 384, 495
RseI CAYNNNNRTG 1 cut(s) 441
SalI GTCGAC 1 cut(s) 517
SaqAI TTAA 1 cut(s) 29
SatI GCNGC 7 cut(s) 41, 44, 97, 166, 174, 177, 653
Sau3AI GATC 3 cut(s) 159, 583, 698
Sau96I GGNCC 5 cut(s) 63, 145, 609, 641, 757
SchI GAGTC 4 cut(s) 243, 341, 436, 539
ScrFI CCNGG 5 cut(s) 314, 618, 707, 745, 761
SfaNI GCATC 1 cut(s) 741
SgrDI CGTCGACG 1 cut(s) 517
SinI GGWCC 3 cut(s) 63, 145, 757
SmiMI CAYNNNNRTG 1 cut(s) 441
Sse9I AATT 1 cut(s) 577
SspMI CTAG 1 cut(s) 819
StyD4I CCNGG 5 cut(s) 312, 616, 705, 743, 759
TaaI ACNGT 2 cut(s) 109, 383
TaiI ACGT 7 cut(s) 56, 299, 351, 422, 443, 524, 755
TaqI TCGA 3 cut(s) 195, 425, 518
TasI AATT 1 cut(s) 577
TatI WGTACW 1 cut(s) 494
TauI GCSGC 4 cut(s) 99, 176, 179, 655
TfiI GAWTC 2 cut(s) 318, 532
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TseFI GTSAC 3 cut(s) 292, 349, 677
TseI GCWGC 3 cut(s) 40, 43, 165
Tsp45I GTSAC 3 cut(s) 292, 349, 677
TspGWI ACGGA 1 cut(s) 64
VpaK11BI GGWCC 3 cut(s) 63, 145, 757
XapI RAATTY 1 cut(s) 577
XceI RCATGY 2 cut(s) 838, 847
XmiI GTMKAC 1 cut(s) 518
XspI CTAG 1 cut(s) 819
ZraI GACGTC 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.