Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0007689

Physical Location & Seq

Genomic Coordinates Reverse (-)

9273576 .. 9276534

Gene Structure 2959 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00288490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1884 bp

ATGGCCGTTACTCCCAACCTCCGCCTCGTAACGGCGTCGCTGCTGCTCCTTTCGTGCGCGTGGACCTCACTCTCCGTCCAGCTCTGCCCACCCTGCGGCAACACCACCGTCCCCTACCCTTTCAGCACCTCCCCCACCTGCGGAGACCAGTCGTACAAGATCCGCTGCGACGCGGCGGCAGGCTCCCTCCTCTTCGACACCCTCAACAACTCCTACCCGATCGCCTCCATCTCCCCCTCGGCCCAACGACTCGTCGTACGCCCCTCCAGCTTCGTGTCGTCCAACTCCTGCGTCACGTCGGATATCGTCCACCAGGGAATCCAGCTCAACGAGTCCCTCCCCTTCAACGTCACCAGTAGCAACACCATCTTGTACCTCAACTGCACGGACACTCTTCTCCGGTCGCCGTTGAACTGCACGTCGTCGAGTCTCTGTCATACGTATGTGAATAATACTAACGCGGTCGGGTCGTGCCAGAGGGCTCCGATTTGCTGTACGTTTCGGGCGGGAGGGTCGTCGACGTCGTATATGATTCGGGTCAGGGACTCGGGCTGCAGCGCTTATACGAGTTTTGTGAATTTGGATCCGGTTTTGCCCGTGAACCGGTGGCCCGAACCGGGAGTGGAGATACAGTGGGTGGCGCCGAGGGAGCCGGTGTGTACTGCGCAGTCTGATTGTGACGGGAAGTCGGTGTGTGGATCCGATCCGGTTCAGAATGGGGTACGGAGGTGCTTCTGTAACTCCGGGTTTTCGTGGGACCCGGTTTCAGGGTTTTGCGTTGATGATAGTGTTGGTTCAAAGGACAGGACTGGACTTATAGCAGGTTTGACTTCTGGCATTGGCGCATCACTACTTGCAGCCATCATTGCCATTGTACTTTACAAGCGCCACAGGCGCATCAAAGAAGCTCAAGACCGCCTGACCAAAGAGCGAGAAGCAATCCTAAATGCGAATGGAGGCAGAGCAGCAAAAGTCTTCACCGGAAAAGAGATTAAAAAGGCAACAAACAACTTCTCCAGAGACCTCCTCTTAGGTGCTGGTGGATATGGTGAAGTCTACAAAGGAACACTTGAAGATGGCACTGTCGTGGCTGTGAAGATCGCGAAGATTGGAAACACGAAAGGCACTGATCAAGTCCTCAATGAGGTCCGAATTCTGTGCCAAGTTAACCACAGGAGTCTTGTGCACTTACTCGGTTGTTGTGTGGAGTTAGAGCAGCCTATTATGGTTTATGAGTACATTGAGAATGGAACTCTACTTGAGCATTTCCAAGCTCGAAAAGGTGTTGGTCGAACACCTCTCTCGTGGACTCAACGTCTCCAAATTGCTCATGACACTGCTGAGGGTCTTGCTTATCTCCACTTCTCAGCTGTCCCTCCAATTTATCACCGTGACGTCAAGTCTAGTAACATTCTTCTTGATAACAAATTGAATGCCAAGGTTGCTGATTTTGGACTATCACGATTGGCTCATACTGATATGAGTCACATTTCAACATGTGCTCAGGGAACTCTTGGATACCTTGATCCTGAATATTACAGAAATTATCAATTGACTGATAAGAGTGATGTCTACAGCTTTGGTGTGGTGCTGTTGGAGCTGATGTCCTCGCAGAAAGCTATAGACTTCAACAGGGACCCCGATGATGTGAACCTTGCAGTTTATGTGCAGAGGTTGATGGCGGAAGAGAAGCTAATGGATGCGATTGATCCAATGCTGAAAGAAGGAGCTACAGCTTTGGAGCTGGACACCATGAAGGCATTGGGGTTCTTGGCATTGGGTTGCTTAGAGGAACGCAGACAGAATAGGCCTTCGATGAAAGAAGTTGTGGAGGAGATTGAGTACATTACAAGCATTGCCACAGCAAAGGTCAACGACGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

627

Amino Acids

68.41

Weight (kDa)

6.17

Isoelectric Point (pI)

36.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	29 - 86	3.1e-09	Wall-associated receptor kinase galacturonan-binding
PK_Tyr_Ser-Thr	PF07714	343 - 613	9.3e-47	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	343 - 611	1.4e-42	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007689)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02070
fragaria_vesca	FvH4_7g23160 FvH4_7g23250 FvH4_7g23370
malus_domestica	MD01G1139800.v1.1
prunus_persica	Prupe.2G240400_v2.0.a1 Prupe.6G261200_v2.0.a1
pyrus_communis	pycom01g16030 pycom08g15310
rosa_chinensis	RchiOBHm_Chr1g0368501 RchiOBHm_Chr3g0483591
rosa_laevigata	RLG00000027208
rosa_multiflora	Rmu_co8210558.1_g000001 Rmu_co8455625.1_g000001 Rmu_sc0006908.1_g000003 Rmu_sc0011394.1_g000011
rosa_roxburghii	Rroxscaffold_4G00288490
rosa_rugosa	Rorug01G0342300.1
rosa_samantha	Rh1AG349700 Rh1BG312700 Rh1BG312800 Rh1CG327600 Rh1DG344200 Rh1DG344300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	146
AatII	GACGTC	2 cut(s)	524, 1398
Acc16I	TGCGCA	1 cut(s)	666
Acc36I	ACCTGC	2 cut(s)	146, 812
AccB1I	GGYRCC	1 cut(s)	640
AccI	GTMKAC	3 cut(s)	518, 1056, 1572
AccII	CGCG	4 cut(s)	59, 173, 461, 1103
AclWI	GGATC	8 cut(s)	154, 578, 591, 693, 698, 706, 1520, 1703
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	2 cut(s)	577, 1152
AcyI	GRCGYC	4 cut(s)	35, 521, 641, 1395
AfaI	GTAC	9 cut(s)	155, 258, 374, 496, 661, 723, 876, 1238, 1844
AfeI	AGCGCT	1 cut(s)	559
AfiI	CCNNNNNNNGG	7 cut(s)	31, 95, 140, 603, 617, 767, 1144
AflIII	ACRYGT	1 cut(s)	1496
AgeI	ACCGGT	1 cut(s)	603
AgsI	TTSAA	7 cut(s)	346, 412, 798, 1073, 1432, 1494, 1630
AhdI	GACNNNNNGTC	3 cut(s)	685, 1314, 1399
AjiI	CACGTC	2 cut(s)	297, 420
AjnI	CCWGG	1 cut(s)	312
AleI	CACNNNNGTG	1 cut(s)	1085
Alw21I	GWGCWC	2 cut(s)	1188, 1504
Alw26I	GTCTC	4 cut(s)	138, 434, 1014, 1322
Alw44I	GTGCAC	1 cut(s)	1184
AlwI	GGATC	8 cut(s)	154, 578, 591, 693, 698, 706, 1520, 1703
Ama87I	CYCGRG	1 cut(s)	547
Aor51HI	AGCGCT	1 cut(s)	559
AoxI	GGCC	4 cut(s)	3, 240, 608, 1808
ApaLI	GTGCAC	1 cut(s)	1184
ApeKI	GCWGC	8 cut(s)	40, 43, 165, 552, 555, 857, 965, 1216
ApoI	RAATTY	2 cut(s)	577, 1152
AsiGI	ACCGGT	1 cut(s)	603
AspLEI	GCGC	7 cut(s)	59, 560, 643, 667, 845, 888, 897
AspS9I	GGNCC	6 cut(s)	63, 241, 609, 757, 1147, 1636
AsuC2I	CCSGG	3 cut(s)	618, 745, 761
AsuHPI	GGTGA	4 cut(s)	343, 970, 1061, 1379
AvaI	CYCGRG	1 cut(s)	547
AvaII	GGWCC	4 cut(s)	63, 757, 1147, 1636
BaeGI	GKGCMC	1 cut(s)	1188
BaeI	ACNNNNGTAYC	2 cut(s)	713, 746
BamHI	GGATCC	2 cut(s)	583, 698
BanI	GGYRCC	1 cut(s)	640
BanII	GRGCYC	1 cut(s)	484
BauI	CACGAG	1 cut(s)	1303
BbsI	GAAGAC	1 cut(s)	967
Bbv12I	GWGCWC	2 cut(s)	1188, 1504
BbvCI	CCTCAGC	1 cut(s)	1341
BbvI	GCAGC	8 cut(s)	27, 30, 152, 539, 567, 869, 977, 1228
BccI	CCATC	5 cut(s)	236, 374, 869, 1070, 1672
BceAI	ACGGC	2 cut(s)	48, 391
BcgI	CGANNNNNNTGC	2 cut(s)	1140, 1174
BciT130I	CCWGG	1 cut(s)	314
BciVI	GTATCC	1 cut(s)	1511
BclI	TGATCA	1 cut(s)	1129
BcnI	CCSGG	3 cut(s)	618, 745, 761
BcoDI	GTCTC	4 cut(s)	138, 434, 1014, 1322
BfaI	CTAG	1 cut(s)	1404
BfmI	CTRYAG	4 cut(s)	553, 1573, 1620, 1731
BfoI	RGCGCY	3 cut(s)	561, 644, 889
BfuAI	ACCTGC	2 cut(s)	146, 812
BfuI	GTATCC	1 cut(s)	1511
Bme1390I	CCNGG	4 cut(s)	314, 618, 745, 761
Bme18I	GGWCC	4 cut(s)	63, 757, 1147, 1636
BmeRI	GACNNNNNGTC	3 cut(s)	685, 1314, 1399
BmeT110I	CYCGRG	1 cut(s)	547
BmgBI	CACGTC	2 cut(s)	297, 420
BmgT120I	GGNCC	6 cut(s)	63, 241, 609, 757, 1147, 1636
BmrFI	CCNGG	4 cut(s)	314, 618, 745, 761
BmsI	GCATC	3 cut(s)	854, 906, 1690
BpiI	GAAGAC	1 cut(s)	967
BplI	GAGNNNNNCTC	2 cut(s)	1011, 1043
BpmI	CTGGAG	2 cut(s)	250, 1000
Bpu10I	CCTNAGC	2 cut(s)	1341, 1503
BpuEI	CTTGAG	2 cut(s)	894, 1280
BpuMI	CCSGG	3 cut(s)	618, 745, 761
BsaAI	YACGTR	1 cut(s)	441
BsaHI	GRCGYC	4 cut(s)	35, 521, 641, 1395
BsaI	GGTCTC	2 cut(s)	138, 1014
BsaJI	CCNNGG	4 cut(s)	237, 313, 644, 1437
BsaWI	WCCGGW	5 cut(s)	399, 586, 603, 706, 980
Bsc4I	CCNNNNNNNGG	7 cut(s)	31, 95, 140, 603, 617, 767, 1144
Bse118I	RCCGGY	2 cut(s)	603, 652
Bse1I	ACTGG	3 cut(s)	148, 354, 814
Bse3DI	GCAATG	2 cut(s)	864, 1854
BseBI	CCWGG	1 cut(s)	314
BseDI	CCNNGG	4 cut(s)	237, 313, 644, 1437
BseGI	GGATG	1 cut(s)	1705
BseLI	CCNNNNNNNGG	7 cut(s)	31, 95, 140, 603, 617, 767, 1144
BseMI	GCAATG	2 cut(s)	864, 1854
BseMII	CTCAG	3 cut(s)	1332, 1380, 1517
BseNI	ACTGG	3 cut(s)	148, 354, 814
BseRI	GAGGAG	3 cut(s)	179, 1016, 1847
BseSI	GKGCMC	1 cut(s)	1188
BseXI	GCAGC	8 cut(s)	27, 30, 152, 539, 567, 869, 977, 1228
BsgI	GTGCAG	3 cut(s)	367, 400, 1688
Bsh1236I	CGCG	4 cut(s)	59, 173, 461, 1103
Bsh1285I	CGRYCG	3 cut(s)	222, 404, 465
BshFI	GGCC	4 cut(s)	5, 242, 610, 1810
BshNI	GGYRCC	1 cut(s)	640
BshTI	ACCGGT	1 cut(s)	603
BsiEI	CGRYCG	3 cut(s)	222, 404, 465
BsiHKAI	GWGCWC	2 cut(s)	1188, 1504
BsiHKCI	CYCGRG	1 cut(s)	547
BsiSI	CCGG	9 cut(s)	400, 587, 604, 617, 653, 707, 744, 761, 981
BsiWI	CGTACG	1 cut(s)	256
BslFI	GGGAC	6 cut(s)	95, 319, 557, 770, 1358, 1649
BslI	CCNNNNNNNGG	7 cut(s)	31, 95, 140, 603, 617, 767, 1144
BsmAI	GTCTC	4 cut(s)	138, 434, 1014, 1322
BsmBI	CGTCTC	1 cut(s)	1322
BsmFI	GGGAC	6 cut(s)	95, 319, 557, 770, 1358, 1649
BsmI	GAATGC	1 cut(s)	1438
BsnI	GGCC	4 cut(s)	5, 242, 610, 1810
Bso31I	GGTCTC	2 cut(s)	138, 1014
BsoBI	CYCGRG	1 cut(s)	547
Bsp1286I	GDGCHC	3 cut(s)	484, 1188, 1504
Bsp143I	GATC	9 cut(s)	159, 219, 583, 698, 703, 1098, 1129, 1525, 1708
Bsp68I	TCGCGA	1 cut(s)	1103
BspANI	GGCC	4 cut(s)	5, 242, 610, 1810
BspCNI	CTCAG	3 cut(s)	1333, 1379, 1516
BspFNI	CGCG	4 cut(s)	59, 173, 461, 1103
BspHI	TCATGA	1 cut(s)	1330
BspMAI	CTGCAG	1 cut(s)	557
BspMI	ACCTGC	2 cut(s)	146, 812
BspPI	GGATC	8 cut(s)	154, 578, 591, 693, 698, 706, 1520, 1703
BspT107I	GGYRCC	1 cut(s)	640
BspTNI	GGTCTC	2 cut(s)	138, 1014
BsrDI	GCAATG	2 cut(s)	864, 1854
BsrFI	RCCGGY	2 cut(s)	603, 652
BsrI	ACTGG	3 cut(s)	148, 354, 814
BssAI	RCCGGY	2 cut(s)	603, 652
BssECI	CCNNGG	4 cut(s)	237, 313, 644, 1437
BssMI	GATC	9 cut(s)	159, 219, 583, 698, 703, 1098, 1129, 1525, 1708
BssNI	GRCGYC	4 cut(s)	35, 521, 641, 1395
BssSI	CACGAG	1 cut(s)	1303
BssT1I	CCWWGG	1 cut(s)	1437
Bst2BI	CACGAG	1 cut(s)	1303
Bst2UI	CCWGG	1 cut(s)	314
Bst4CI	ACNGT	4 cut(s)	109, 633, 1084, 1391
Bst6I	CTCTTC	3 cut(s)	197, 399, 1680
BstACI	GRCGYC	4 cut(s)	35, 521, 641, 1395
BstBAI	YACGTR	1 cut(s)	441
BstC8I	GCNNGC	1 cut(s)	181
BstDEI	CTNAG	5 cut(s)	1030, 1341, 1366, 1503, 1786
BstENI	CCTNNNNNAGG	1 cut(s)	1142
BstF5I	GGATG	1 cut(s)	1705
BstFNI	CGCG	4 cut(s)	59, 173, 461, 1103
BstH2I	RGCGCY	3 cut(s)	561, 644, 889
BstHHI	GCGC	7 cut(s)	59, 560, 643, 667, 845, 888, 897
BstKTI	GATC	9 cut(s)	162, 222, 586, 701, 706, 1101, 1132, 1528, 1711
BstMAI	GTCTC	4 cut(s)	138, 434, 1014, 1322
BstMBI	GATC	9 cut(s)	159, 219, 583, 698, 703, 1098, 1129, 1525, 1708
BstMCI	CGRYCG	3 cut(s)	222, 404, 465
BstMWI	GCNNNNNNNGC	8 cut(s)	93, 267, 649, 866, 892, 894, 1442, 1597
BstNI	CCWGG	1 cut(s)	314
BstNSI	RCATGY	1 cut(s)	1500
BstSCI	CCNGG	4 cut(s)	312, 616, 743, 759
BstSFI	CTRYAG	4 cut(s)	553, 1573, 1620, 1731
BstSLI	GKGCMC	1 cut(s)	1188
BstSNI	TACGTA	1 cut(s)	441
BstUI	CGCG	4 cut(s)	59, 173, 461, 1103
BstV1I	GCAGC	8 cut(s)	27, 30, 152, 539, 567, 869, 977, 1228
BstV2I	GAAGAC	1 cut(s)	967
BstX2I	RGATCY	3 cut(s)	159, 583, 698
BstYI	RGATCY	3 cut(s)	159, 583, 698
BsuI	GTATCC	1 cut(s)	1511
BsuRI	GGCC	4 cut(s)	5, 242, 610, 1810
BtrI	CACGTC	2 cut(s)	297, 420
BtsCI	GGATG	1 cut(s)	1705
BtsI	GCAGTG	1 cut(s)	1335
BtsIMutI	CAGTG	4 cut(s)	638, 1080, 1125, 1335
BtuMI	TCGCGA	1 cut(s)	1103
BveI	ACCTGC	2 cut(s)	146, 812
Cac8I	GCNNGC	1 cut(s)	181
CciI	TCATGA	1 cut(s)	1330
CfoI	GCGC	7 cut(s)	59, 560, 643, 667, 845, 888, 897
Cfr10I	RCCGGY	2 cut(s)	603, 652
Cfr13I	GGNCC	6 cut(s)	63, 241, 609, 757, 1147, 1636
CseI	GACGC	3 cut(s)	24, 179, 280
Csp6I	GTAC	9 cut(s)	154, 257, 373, 495, 660, 722, 875, 1237, 1843
CspAI	ACCGGT	1 cut(s)	603
CviAII	CATG	3 cut(s)	1331, 1497, 1753
CviQI	GTAC	9 cut(s)	154, 257, 373, 495, 660, 722, 875, 1237, 1843
DdeI	CTNAG	5 cut(s)	1030, 1341, 1366, 1503, 1786
DinI	GGCGCC	1 cut(s)	642
DpnI	GATC	9 cut(s)	161, 221, 585, 700, 705, 1100, 1131, 1527, 1710
DpnII	GATC	9 cut(s)	159, 219, 583, 698, 703, 1098, 1129, 1525, 1708
DriI	GACNNNNNGTC	3 cut(s)	685, 1314, 1399
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	3 cut(s)	197, 399, 1680
Eam1105I	GACNNNNNGTC	3 cut(s)	685, 1314, 1399
EarI	CTCTTC	3 cut(s)	197, 399, 1680
EciI	GGCGGA	2 cut(s)	11, 1697
Eco105I	TACGTA	1 cut(s)	441
Eco130I	CCWWGG	1 cut(s)	1437
Eco147I	AGGCCT	1 cut(s)	1810
Eco24I	GRGCYC	1 cut(s)	484
Eco31I	GGTCTC	2 cut(s)	138, 1014
Eco32I	GATATC	1 cut(s)	304
Eco47I	GGWCC	4 cut(s)	63, 757, 1147, 1636
Eco47III	AGCGCT	1 cut(s)	559
Eco88I	CYCGRG	1 cut(s)	547
EcoNI	CCTNNNNNAGG	1 cut(s)	1142
EcoO109I	RGGNCCY	2 cut(s)	757, 1636
EcoRI	GAATTC	1 cut(s)	1152
EcoRII	CCWGG	1 cut(s)	312
EcoRV	GATATC	1 cut(s)	304
EcoT14I	CCWWGG	1 cut(s)	1437
EcoT38I	GRGCYC	1 cut(s)	484
EgeI	GGCGCC	1 cut(s)	642
EheI	GGCGCC	1 cut(s)	642
ErhI	CCWWGG	1 cut(s)	1437
Esp3I	CGTCTC	1 cut(s)	1322
FaeI	CATG	3 cut(s)	1334, 1500, 1756
FalI	AAGNNNNNCTT	2 cut(s)	1053, 1085
FaqI	GGGAC	6 cut(s)	95, 319, 557, 770, 1358, 1649
FatI	CATG	3 cut(s)	1330, 1496, 1752
FauI	CCCGC	1 cut(s)	499
FbaI	TGATCA	1 cut(s)	1129
FblI	GTMKAC	3 cut(s)	518, 1056, 1572
FokI	GGATG	1 cut(s)	1712
FriOI	GRGCYC	1 cut(s)	484
FspBI	CTAG	1 cut(s)	1404
FspI	TGCGCA	1 cut(s)	666
GlaI	GCGC	7 cut(s)	58, 559, 642, 666, 844, 887, 896
GsuI	CTGGAG	2 cut(s)	250, 1000
HaeII	RGCGCY	3 cut(s)	561, 644, 889
HaeIII	GGCC	4 cut(s)	5, 242, 610, 1810
HapII	CCGG	9 cut(s)	400, 587, 604, 617, 653, 707, 744, 761, 981
HgaI	GACGC	3 cut(s)	24, 179, 280
HhaI	GCGC	7 cut(s)	59, 560, 643, 667, 845, 888, 897
Hin1I	GRCGYC	4 cut(s)	35, 521, 641, 1395
Hin1II	CATG	3 cut(s)	1334, 1500, 1756
Hin6I	GCGC	7 cut(s)	57, 558, 641, 665, 843, 886, 895
HinP1I	GCGC	7 cut(s)	57, 558, 641, 665, 843, 886, 895
HincII	GTYRAC	3 cut(s)	519, 1168, 1873
HindII	GTYRAC	3 cut(s)	519, 1168, 1873
HinfI	GANTC	9 cut(s)	249, 318, 332, 427, 532, 545, 1177, 1309, 1483
HpaI	GTTAAC	1 cut(s)	1168
HpaII	CCGG	9 cut(s)	400, 587, 604, 617, 653, 707, 744, 761, 981
HphI	GGTGA	4 cut(s)	343, 970, 1061, 1379
Hpy188I	TCNGA	6 cut(s)	301, 486, 673, 703, 714, 1151
Hpy188III	TCNNGA	7 cut(s)	911, 1017, 1102, 1331, 1418, 1461, 1529
HpyAV	CCTTC	4 cut(s)	352, 1718, 1750, 1821
HpyCH4III	ACNGT	4 cut(s)	109, 633, 1084, 1391
HpyCH4IV	ACGT	8 cut(s)	296, 348, 419, 440, 497, 521, 1315, 1395
HpyCH4V	TGCA	7 cut(s)	384, 417, 555, 857, 1186, 1658, 1669
HpyF10VI	GCNNNNNNNGC	8 cut(s)	93, 267, 649, 866, 892, 894, 1442, 1597
HpyF3I	CTNAG	5 cut(s)	1030, 1341, 1366, 1503, 1786
HpySE526I	ACGT	8 cut(s)	296, 348, 419, 440, 497, 521, 1315, 1395
Hsp92I	GRCGYC	4 cut(s)	35, 521, 641, 1395
Hsp92II	CATG	3 cut(s)	1334, 1500, 1756
HspAI	GCGC	7 cut(s)	57, 558, 641, 665, 843, 886, 895
KasI	GGCGCC	1 cut(s)	640
KflI	GGGWCCC	2 cut(s)	757, 1636
Ksp22I	TGATCA	1 cut(s)	1129
KspAI	GTTAAC	1 cut(s)	1168
Kzo9I	GATC	9 cut(s)	159, 219, 583, 698, 703, 1098, 1129, 1525, 1708
LmnI	GCTCC	7 cut(s)	51, 188, 487, 649, 1597, 1727, 1741
Lsp1109I	GCAGC	8 cut(s)	27, 30, 152, 539, 567, 869, 977, 1228
LweI	GCATC	3 cut(s)	854, 906, 1690
MaeI	CTAG	1 cut(s)	1404
MaeII	ACGT	8 cut(s)	296, 348, 419, 440, 497, 521, 1315, 1395
MaeIII	GTNAC	9 cut(s)	7, 28, 292, 349, 677, 737, 1391, 1406, 1484
MalI	GATC	9 cut(s)	161, 221, 585, 700, 705, 1100, 1131, 1527, 1710
MboI	GATC	9 cut(s)	159, 219, 583, 698, 703, 1098, 1129, 1525, 1708
MboII	GAAGA	8 cut(s)	184, 386, 967, 1085, 1108, 1117, 1406, 1697
MfeI	CAATTG	1 cut(s)	1550
MflI	RGATCY	3 cut(s)	159, 583, 698
MhlI	GDGCHC	3 cut(s)	484, 1188, 1504
MluCI	AATT	7 cut(s)	577, 1152, 1323, 1380, 1427, 1543, 1550
Mly113I	GGCGCC	1 cut(s)	641
MlyI	GAGTC	7 cut(s)	243, 341, 436, 539, 1186, 1303, 1492
MmeI	TCCRAC	3 cut(s)	279, 306, 1575
MseI	TTAA	2 cut(s)	993, 1167
MslI	CAYNNNNRTG	2 cut(s)	441, 1085
MspA1I	CMGCKG	2 cut(s)	165, 1370
MspI	CCGG	9 cut(s)	400, 587, 604, 617, 653, 707, 744, 761, 981
MspR9I	CCNGG	4 cut(s)	314, 618, 745, 761
MunI	CAATTG	1 cut(s)	1550
Mva1269I	GAATGC	1 cut(s)	1438
MvaI	CCWGG	1 cut(s)	314
MvnI	CGCG	4 cut(s)	59, 173, 461, 1103
MwoI	GCNNNNNNNGC	8 cut(s)	93, 267, 649, 866, 892, 894, 1442, 1597
NarI	GGCGCC	1 cut(s)	641
NciI	CCSGG	3 cut(s)	618, 745, 761
NdeII	GATC	9 cut(s)	159, 219, 583, 698, 703, 1098, 1129, 1525, 1708
NlaIII	CATG	3 cut(s)	1334, 1500, 1756
NmeAIII	GCCGAG	2 cut(s)	218, 669
NmuCI	GTSAC	5 cut(s)	292, 349, 677, 1391, 1484
NruI	TCGCGA	1 cut(s)	1103
NsbI	TGCGCA	1 cut(s)	666
NspI	RCATGY	1 cut(s)	1500
OliI	CACNNNNGTG	1 cut(s)	1085
PagI	TCATGA	1 cut(s)	1330
PaqCI	CACCTGC	1 cut(s)	146
PceI	AGGCCT	1 cut(s)	1810
PciI	ACATGT	1 cut(s)	1496
PcsI	WCGNNNNNNNCGW	1 cut(s)	521
PctI	GAATGC	1 cut(s)	1438
PfeI	GAWTC	2 cut(s)	318, 532
Pfl23II	CGTACG	1 cut(s)	256
PinAI	ACCGGT	1 cut(s)	603
Ple19I	CGATCG	1 cut(s)	222
PleI	GAGTC	7 cut(s)	243, 340, 435, 539, 1185, 1303, 1491
PluTI	GGCGCC	1 cut(s)	644
PpsI	GAGTC	7 cut(s)	243, 340, 435, 539, 1185, 1303, 1491
Ppu21I	YACGTR	1 cut(s)	441
PpuMI	RGGWCCY	2 cut(s)	757, 1636
PscI	ACATGT	1 cut(s)	1496
Psp5II	RGGWCCY	2 cut(s)	757, 1636
Psp6I	CCWGG	1 cut(s)	312
PspGI	CCWGG	1 cut(s)	312
PspLI	CGTACG	1 cut(s)	256
PspPI	GGNCC	6 cut(s)	63, 241, 609, 757, 1147, 1636
PspPPI	RGGWCCY	2 cut(s)	757, 1636
PstI	CTGCAG	1 cut(s)	557
PsuI	RGATCY	3 cut(s)	159, 583, 698
PvuI	CGATCG	1 cut(s)	222
PvuII	CAGCTG	1 cut(s)	1370
RruI	TCGCGA	1 cut(s)	1103
RsaI	GTAC	9 cut(s)	155, 258, 374, 496, 661, 723, 876, 1238, 1844
RsaNI	GTAC	9 cut(s)	154, 257, 373, 495, 660, 722, 875, 1237, 1843
RseI	CAYNNNNRTG	2 cut(s)	441, 1085
SalI	GTCGAC	1 cut(s)	517
SaqAI	TTAA	2 cut(s)	993, 1167
Sau3AI	GATC	9 cut(s)	159, 219, 583, 698, 703, 1098, 1129, 1525, 1708
Sau96I	GGNCC	6 cut(s)	63, 241, 609, 757, 1147, 1636
SchI	GAGTC	7 cut(s)	243, 341, 436, 539, 1186, 1303, 1492
ScrFI	CCNGG	4 cut(s)	314, 618, 745, 761
SduI	GDGCHC	3 cut(s)	484, 1188, 1504
SfaNI	GCATC	3 cut(s)	854, 906, 1690
SfcI	CTRYAG	4 cut(s)	553, 1573, 1620, 1731
SfoI	GGCGCC	1 cut(s)	642
SgrDI	CGTCGACG	1 cut(s)	517
SinI	GGWCC	4 cut(s)	63, 757, 1147, 1636
SmiMI	CAYNNNNRTG	2 cut(s)	441, 1085
SmlI	CTYRAG	2 cut(s)	909, 1259
SmoI	CTYRAG	2 cut(s)	909, 1259
SnaBI	TACGTA	1 cut(s)	441
Sse9I	AATT	7 cut(s)	577, 1152, 1323, 1380, 1427, 1543, 1550
SseBI	AGGCCT	1 cut(s)	1810
SspDI	GGCGCC	1 cut(s)	640
SspI	AATATT	1 cut(s)	1535
SspMI	CTAG	1 cut(s)	1404
StuI	AGGCCT	1 cut(s)	1810
StyD4I	CCNGG	4 cut(s)	312, 616, 743, 759
StyI	CCWWGG	1 cut(s)	1437
TaaI	ACNGT	4 cut(s)	109, 633, 1084, 1391
TaiI	ACGT	8 cut(s)	299, 351, 422, 443, 500, 524, 1318, 1398
TaqI	TCGA	6 cut(s)	195, 425, 518, 1276, 1291, 1814
TasI	AATT	7 cut(s)	577, 1152, 1323, 1380, 1427, 1543, 1550
TatI	WGTACW	4 cut(s)	659, 874, 1236, 1842
TauI	GCSGC	3 cut(s)	99, 176, 179
TfiI	GAWTC	2 cut(s)	318, 532
Tru1I	TTAA	2 cut(s)	993, 1167
Tru9I	TTAA	2 cut(s)	993, 1167
TscAI	CASTG	4 cut(s)	638, 1087, 1132, 1342
TseFI	GTSAC	5 cut(s)	292, 349, 677, 1391, 1484
TseI	GCWGC	8 cut(s)	40, 43, 165, 552, 555, 857, 965, 1216
Tsp45I	GTSAC	5 cut(s)	292, 349, 677, 1391, 1484
TspDTI	ATGAA	2 cut(s)	1769, 1832
TspGWI	ACGGA	3 cut(s)	64, 401, 739
TspRI	CASTG	4 cut(s)	638, 1087, 1132, 1342
VneI	GTGCAC	1 cut(s)	1184
VpaK11BI	GGWCC	4 cut(s)	63, 757, 1147, 1636
XagI	CCTNNNNNAGG	1 cut(s)	1142
XapI	RAATTY	2 cut(s)	577, 1152
XceI	RCATGY	1 cut(s)	1500
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1759
XmiI	GTMKAC	3 cut(s)	518, 1056, 1572
XspI	CTAG	1 cut(s)	1404
ZraI	GACGTC	2 cut(s)	522, 1396

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.