MD01G1159800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0015143
Physical Location & Seq
Forward (+)
26595265 .. 26596991
1727 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1159800.v1.1.491

Sequence Viewer

Length: 1491 bp
ATGGCTTCTTGTGGCTTTCCTGACATATTTTCCTGGATTCAAACCCTCCCATCGATCGCTCAATGGCCAACACCTTCCATGTCCATATCCATATGTTCTTCAAGTTCATCACAACCATCTCTCAAACTCTGCGTTTCCAAAAATCTCCAAAACCCTACTTTCTCTTTTGCTATACTAGCAGACTTCAATCTCCCCATCTCCCTTTGGACCTCAAAGCAGTTCAAATTCAACCCTAAATCCACCAACTTAATAACCCAAGAAGCACTTTCCAGCCTCTTAGTCAATTTCATTGAAGATGTCCTTCGTTACGGCTCGAGCAAGAACAATTTCTTGATCAAATTTCTCAAATTCGACTCCATTGCCAACTTTGGCGATGTTTTTAACCTCGCATTTGTCACGCTCGTCTTTCTTGTTTGCATCTATGAAGCCCCCGCGGGTCTCCGGTCGGGATGGCTCAATACTCTCAAGAATTACTTGGCTAATTGTTGGTCAAGACAATCATCAAAGACCCTAATGAAGTTATTAGGATCCAATTTGGAGGAGGAATGGATGAGGTCAATCAATCTTGCAATCACCAATTGGATTCTTGAGCTTCAAGCCACCCAGGGAACCCTCATGAAAACACCTTCACCACTCTTCTCTTATGGTTTTTCAGCATTTGGGTTGTGGAAAGTCCAATTGTATTGTCCTCTCATAGCCATGGACATTGCCAAATCCAGCAATATCTCTGTTGATGAAAGGCTCCAATTCTCTCTGAATTTCCACCAGCTTGAGGGTGTGCTTCAATTCAATTACAATGTCATGGTTCAAGAGAGGTGGATTGATGTGATGCTAAACATTGATAACATAAGGTGTGATGTGATCAGTCTTGTGAATGAGAGACTAATGAACGAGCAAGGCGCCGGGGTATCTGAAAAGCACTTCCCTTCAAGAATTTCATTACAAGTTACTCCAACTACACAAACAAATGTGCTAAGCGTTTCAGTTGGCAAGTCTTCTGAAAACCCTGCAATAGAGATTGGCAAGGAAAAAGGCATAGAAGGCTCGTTTGAACCCCCAAACCCTTACTTGGCGCTCAAGGTATCAGCTAGCGAGACCATAACAATGAGCTTAAAACCCTGGAAGTTTGAGGAATCAGTGTACGGATATAGCGCAAATTTGAACTGGTTTCTTCATGACAGTGTGGATGGAAGAGAGGTCTTCTCTTCCAAGCCATCAAAGTTTCAATTGATCAACCCCAAAGCTTGGTTCAAAGATAGATACTCTAGTGCATATAGGCCTTTCACAAGGCAAGGAGGGGTTGTTTTTGCTGGTGATGAGTATGGAGAGGGTGTGATTTGGAAGGTGGACAAAGCTGCAATGGGAAGAACAATGGATTGGGAAGTTAGAGGGTGGATTTGGTTAACATATTGGCCTAACAAACATAAAACTTTTTATACTGAAACCAGAAGGTTGGAATTTAGAGAAATCCTCACCCTTACAATTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

497

Amino Acids

56.39

Weight (kDa)

8.31

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40390
fragaria_vesca FvH4_7g25370
malus_domestica MD01G1159800.v1.1
prunus_persica Prupe.2G257800_v2.0.a1
pyrus_communis pycom01g17620
rosa_chinensis RchiOBHm_Chr1g0371291
rosa_laevigata RLG00000026973
rosa_multiflora Rmu_sc0016653.1_g000001
rosa_roxburghii Rroxscaffold_4G00285810
rosa_rugosa Rorug01G0362700 Rorug01G0362800
rosa_samantha Rh1AG372800 Rh1BG336300 Rh1CG349700 Rh1DG367400
rosa_wichuraiana Rw1G032790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 899
AccB7I CCANNNNNTGG 1 cut(s) 1245
AccII CGCG 1 cut(s) 434
AciI CCGC 2 cut(s) 432, 434
AclWI GGATC 2 cut(s) 522, 535
AcoI YGGCCR 1 cut(s) 65
AcsI RAATTY 7 cut(s) 224, 338, 347, 757, 933, 1156, 1457
AcyI GRCGYC 1 cut(s) 900
AfaI GTAC 1 cut(s) 1142
AfiI CCNNNNNNNGG 3 cut(s) 772, 1069, 1245
AjnI CCWGG 3 cut(s) 32, 603, 1118
AjuI GAANNNNNNNTTGG 4 cut(s) 1232, 1264, 1360, 1392
AluBI AGCT 6 cut(s) 592, 769, 1088, 1110, 1244, 1355
AluI AGCT 6 cut(s) 592, 769, 1088, 1110, 1244, 1355
Alw26I GTCTC 3 cut(s) 443, 874, 1088
AlwI GGATC 2 cut(s) 522, 535
Ama87I CYCGRG 1 cut(s) 313
AoxI GGCC 3 cut(s) 65, 1277, 1412
ApeKI GCWGC 1 cut(s) 1355
ApoI RAATTY 7 cut(s) 224, 338, 347, 757, 933, 1156, 1457
Asp700I GAANNNNTTC 1 cut(s) 326
AspLEI GCGC 3 cut(s) 902, 1075, 1154
AspS9I GGNCC 1 cut(s) 207
AsuC2I CCSGG 1 cut(s) 904
AsuHPI GGTGA 4 cut(s) 565, 621, 1325, 1465
AsuNHI GCTAGC 1 cut(s) 1088
AvaI CYCGRG 1 cut(s) 313
AvaII GGWCC 1 cut(s) 207
BaeI ACNNNNGTAYC 2 cut(s) 1252, 1285
BalI TGGCCA 1 cut(s) 67
BamHI GGATCC 1 cut(s) 527
BanI GGYRCC 1 cut(s) 899
BbsI GAAGAC 2 cut(s) 987, 1192
BbvI GCAGC 1 cut(s) 1342
BccI CCATC 6 cut(s) 58, 124, 203, 444, 1181, 1222
BceAI ACGGC 1 cut(s) 325
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BciT130I CCWGG 3 cut(s) 34, 605, 1120
BclI TGATCA 3 cut(s) 333, 861, 1230
BcnI CCSGG 1 cut(s) 904
BcoDI GTCTC 3 cut(s) 443, 874, 1088
BfaI CTAG 3 cut(s) 176, 1089, 1266
BfoI RGCGCY 2 cut(s) 903, 1076
BisI GCNGC 1 cut(s) 1356
BlpI GCTNAGC 1 cut(s) 974
BlsI GCNGC 1 cut(s) 1357
Bme1390I CCNGG 4 cut(s) 34, 605, 904, 1120
Bme18I GGWCC 1 cut(s) 207
BmeT110I CYCGRG 1 cut(s) 313
BmgT120I GGNCC 1 cut(s) 207
BmiI GGNNCC 4 cut(s) 529, 610, 743, 901
BmrFI CCNGG 4 cut(s) 34, 605, 904, 1120
BmsI GCATC 2 cut(s) 426, 819
BmtI GCTAGC 1 cut(s) 1092
BpiI GAAGAC 2 cut(s) 987, 1192
BplI GAGNNNNNCTC 4 cut(s) 1187, 1219, 1455, 1487
Bpu1102I GCTNAGC 1 cut(s) 974
BpuEI CTTGAG 4 cut(s) 449, 608, 791, 1061
BpuMI CCSGG 1 cut(s) 904
Bsa29I ATCGAT 1 cut(s) 53
BsaHI GRCGYC 1 cut(s) 900
BsaI GGTCTC 2 cut(s) 443, 1088
BsaJI CCNNGG 6 cut(s) 432, 603, 604, 699, 903, 1118
BsaWI WCCGGW 1 cut(s) 441
BsaXI ACNNNNNCTCC 4 cut(s) 1287, 1317, 1317, 1347
Bsc4I CCNNNNNNNGG 3 cut(s) 772, 1069, 1245
Bse1I ACTGG 1 cut(s) 1169
Bse3DI GCAATG 3 cut(s) 357, 705, 1365
BseBI CCWGG 3 cut(s) 34, 605, 1120
BseCI ATCGAT 1 cut(s) 53
BseDI CCNNGG 6 cut(s) 432, 603, 604, 699, 903, 1118
BseGI GGATG 3 cut(s) 455, 555, 1192
BseLI CCNNNNNNNGG 3 cut(s) 772, 1069, 1245
BseMI GCAATG 3 cut(s) 357, 705, 1365
BseNI ACTGG 1 cut(s) 1169
BseRI GAGGAG 1 cut(s) 554
BseXI GCAGC 1 cut(s) 1342
Bsh1236I CGCG 1 cut(s) 434
Bsh1285I CGRYCG 2 cut(s) 57, 446
BshFI GGCC 3 cut(s) 67, 1279, 1414
BshNI GGYRCC 1 cut(s) 899
BshVI ATCGAT 1 cut(s) 53
BsiEI CGRYCG 2 cut(s) 57, 446
BsiHKCI CYCGRG 1 cut(s) 313
BsiSI CCGG 2 cut(s) 442, 903
BslI CCNNNNNNNGG 3 cut(s) 772, 1069, 1245
BsmAI GTCTC 3 cut(s) 443, 874, 1088
BsnI GGCC 3 cut(s) 67, 1279, 1414
Bso31I GGTCTC 2 cut(s) 443, 1088
BsoBI CYCGRG 1 cut(s) 313
Bsp143I GATC 5 cut(s) 54, 333, 527, 861, 1230
Bsp1720I GCTNAGC 1 cut(s) 974
Bsp19I CCATGG 1 cut(s) 699
BspACI CCGC 2 cut(s) 432, 434
BspANI GGCC 3 cut(s) 67, 1279, 1414
BspDI ATCGAT 1 cut(s) 53
BspFNI CGCG 1 cut(s) 434
BspHI TCATGA 2 cut(s) 615, 1174
BspLI GGNNCC 4 cut(s) 529, 610, 743, 901
BspOI GCTAGC 1 cut(s) 1092
BspPI GGATC 2 cut(s) 522, 535
BspT107I GGYRCC 1 cut(s) 899
BspTNI GGTCTC 2 cut(s) 443, 1088
BsrDI GCAATG 3 cut(s) 357, 705, 1365
BsrI ACTGG 1 cut(s) 1169
BssECI CCNNGG 6 cut(s) 432, 603, 604, 699, 903, 1118
BssMI GATC 5 cut(s) 54, 333, 527, 861, 1230
BssNI GRCGYC 1 cut(s) 900
BssT1I CCWWGG 1 cut(s) 699
Bst2UI CCWGG 3 cut(s) 34, 605, 1120
Bst4CI ACNGT 1 cut(s) 1181
Bst6I CTCTTC 3 cut(s) 641, 1186, 1210
BstACI GRCGYC 1 cut(s) 900
BstC8I GCNNGC 1 cut(s) 1090
BstDEI CTNAG 2 cut(s) 277, 974
BstDSI CCRYGG 2 cut(s) 432, 699
BstF5I GGATG 3 cut(s) 455, 555, 1192
BstFNI CGCG 1 cut(s) 434
BstH2I RGCGCY 2 cut(s) 903, 1076
BstHHI GCGC 3 cut(s) 902, 1075, 1154
BstKTI GATC 5 cut(s) 57, 336, 530, 864, 1233
BstMAI GTCTC 3 cut(s) 443, 874, 1088
BstMBI GATC 5 cut(s) 54, 333, 527, 861, 1230
BstMCI CGRYCG 2 cut(s) 57, 446
BstMWI GCNNNNNNNGC 2 cut(s) 176, 1041
BstNI CCWGG 3 cut(s) 34, 605, 1120
BstSCI CCNGG 4 cut(s) 32, 603, 902, 1118
BstUI CGCG 1 cut(s) 434
BstV1I GCAGC 1 cut(s) 1342
BstV2I GAAGAC 2 cut(s) 987, 1192
BstX2I RGATCY 1 cut(s) 527
BstXI CCANNNNNNTGG 1 cut(s) 1453
BstYI RGATCY 1 cut(s) 527
Bsu15I ATCGAT 1 cut(s) 53
BsuRI GGCC 3 cut(s) 67, 1279, 1414
BsuTUI ATCGAT 1 cut(s) 53
BtgI CCRYGG 2 cut(s) 432, 699
BtgZI GCGATG 1 cut(s) 387
BtsCI GGATG 3 cut(s) 455, 555, 1192
BtsIMutI CAGTG 2 cut(s) 1143, 1186
Cac8I GCNNGC 1 cut(s) 1090
CciI TCATGA 2 cut(s) 615, 1174
CfoI GCGC 3 cut(s) 902, 1075, 1154
Cfr13I GGNCC 1 cut(s) 207
Cfr42I CCGCGG 1 cut(s) 435
ClaI ATCGAT 1 cut(s) 53
Csp6I GTAC 1 cut(s) 1141
CspCI CAANNNNNGTGG 2 cut(s) 797, 832
CviAII CATG 5 cut(s) 79, 616, 700, 802, 1175
CviQI GTAC 1 cut(s) 1141
DdeI CTNAG 2 cut(s) 277, 974
DinI GGCGCC 1 cut(s) 901
DpnI GATC 5 cut(s) 56, 335, 529, 863, 1232
DpnII GATC 5 cut(s) 54, 333, 527, 861, 1230
EaeI YGGCCR 1 cut(s) 65
Eam1104I CTCTTC 3 cut(s) 641, 1186, 1210
EarI CTCTTC 3 cut(s) 641, 1186, 1210
Eco130I CCWWGG 1 cut(s) 699
Eco147I AGGCCT 1 cut(s) 1279
Eco31I GGTCTC 2 cut(s) 443, 1088
Eco47I GGWCC 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 313
EcoRII CCWGG 3 cut(s) 32, 603, 1118
EcoT14I CCWWGG 1 cut(s) 699
EgeI GGCGCC 1 cut(s) 901
EheI GGCGCC 1 cut(s) 901
ErhI CCWWGG 1 cut(s) 699
FaeI CATG 5 cut(s) 82, 619, 703, 805, 1178
FalI AAGNNNNNCTT 6 cut(s) 249, 281, 285, 317, 458, 490
FatI CATG 5 cut(s) 78, 615, 699, 801, 1174
FauI CCCGC 2 cut(s) 427, 439
FauNDI CATATG 1 cut(s) 92
FbaI TGATCA 3 cut(s) 333, 861, 1230
Fnu4HI GCNGC 1 cut(s) 1356
FokI GGATG 3 cut(s) 462, 562, 1199
Fsp4HI GCNGC 1 cut(s) 1356
FspBI CTAG 3 cut(s) 176, 1089, 1266
GlaI GCGC 3 cut(s) 901, 1074, 1153
GluI GCNGC 1 cut(s) 1356
HaeII RGCGCY 2 cut(s) 903, 1076
HaeIII GGCC 3 cut(s) 67, 1279, 1414
HapII CCGG 2 cut(s) 442, 903
HhaI GCGC 3 cut(s) 902, 1075, 1154
Hin1I GRCGYC 1 cut(s) 900
Hin1II CATG 5 cut(s) 82, 619, 703, 805, 1178
Hin6I GCGC 3 cut(s) 900, 1073, 1152
HinP1I GCGC 3 cut(s) 900, 1073, 1152
HincII GTYRAC 1 cut(s) 1404
HindII GTYRAC 1 cut(s) 1404
HindIII AAGCTT 1 cut(s) 1242
HinfI GANTC 4 cut(s) 37, 353, 583, 1133
HpaI GTTAAC 1 cut(s) 1404
HpaII CCGG 2 cut(s) 442, 903
HphI GGTGA 4 cut(s) 565, 621, 1325, 1465
Hpy166II GTNNAC 3 cut(s) 1141, 1348, 1404
Hpy188I TCNGA 3 cut(s) 756, 913, 1000
Hpy8I GTNNAC 3 cut(s) 1141, 1348, 1404
HpyAV CCTTC 7 cut(s) 84, 311, 636, 936, 1034, 1336, 1443
HpyCH4III ACNGT 1 cut(s) 1181
HpyCH4V TGCA 5 cut(s) 417, 569, 1010, 1271, 1358
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 1041
HpyF3I CTNAG 2 cut(s) 277, 974
Hsp92I GRCGYC 1 cut(s) 900
Hsp92II CATG 5 cut(s) 82, 619, 703, 805, 1178
HspAI GCGC 3 cut(s) 900, 1073, 1152
KasI GGCGCC 1 cut(s) 899
Ksp22I TGATCA 3 cut(s) 333, 861, 1230
KspAI GTTAAC 1 cut(s) 1404
KspI CCGCGG 1 cut(s) 435
Kzo9I GATC 5 cut(s) 54, 333, 527, 861, 1230
LmnI GCTCC 1 cut(s) 747
Lsp1109I GCAGC 1 cut(s) 1342
LweI GCATC 2 cut(s) 426, 819
MaeI CTAG 3 cut(s) 176, 1089, 1266
MaeIII GTNAC 3 cut(s) 305, 394, 946
MalI GATC 5 cut(s) 56, 335, 529, 863, 1232
MboI GATC 5 cut(s) 54, 333, 527, 861, 1230
MboII GAAGA 9 cut(s) 90, 305, 628, 987, 1163, 1192, 1197, 1203, 1377
MfeI CAATTG 4 cut(s) 577, 677, 1226, 1482
MflI RGATCY 1 cut(s) 527
MlsI TGGCCA 1 cut(s) 67
MluNI TGGCCA 1 cut(s) 67
Mly113I GGCGCC 1 cut(s) 900
MlyI GAGTC 1 cut(s) 347
MmeI TCCRAC 2 cut(s) 977, 1434
Mox20I TGGCCA 1 cut(s) 67
MroXI GAANNNNTTC 1 cut(s) 326
MscI TGGCCA 1 cut(s) 67
MseI TTAA 5 cut(s) 248, 381, 1112, 1403, 1489
MslI CAYNNNNRTG 3 cut(s) 698, 1103, 1179
Msp20I TGGCCA 1 cut(s) 67
MspA1I CMGCKG 1 cut(s) 434
MspI CCGG 2 cut(s) 442, 903
MspR9I CCNGG 4 cut(s) 34, 605, 904, 1120
MunI CAATTG 4 cut(s) 577, 677, 1226, 1482
MvaI CCWGG 3 cut(s) 34, 605, 1120
MvnI CGCG 1 cut(s) 434
MwoI GCNNNNNNNGC 2 cut(s) 176, 1041
NarI GGCGCC 1 cut(s) 900
NciI CCSGG 1 cut(s) 904
NcoI CCATGG 1 cut(s) 699
NdeI CATATG 1 cut(s) 92
NdeII GATC 5 cut(s) 54, 333, 527, 861, 1230
NheI GCTAGC 1 cut(s) 1088
NlaIII CATG 5 cut(s) 82, 619, 703, 805, 1178
NlaIV GGNNCC 4 cut(s) 529, 610, 743, 901
NmuCI GTSAC 1 cut(s) 394
PaeR7I CTCGAG 1 cut(s) 313
PagI TCATGA 2 cut(s) 615, 1174
PasI CCCWGGG 1 cut(s) 604
PceI AGGCCT 1 cut(s) 1279
PdmI GAANNNNTTC 1 cut(s) 326
PfeI GAWTC 3 cut(s) 37, 583, 1133
PflMI CCANNNNNTGG 1 cut(s) 1245
PfoI TCCNGGA 1 cut(s) 32
PkrI GCNGC 1 cut(s) 1357
Ple19I CGATCG 1 cut(s) 57
PleI GAGTC 1 cut(s) 347
PluTI GGCGCC 1 cut(s) 903
PpsI GAGTC 1 cut(s) 347
Psp6I CCWGG 3 cut(s) 32, 603, 1118
PspGI CCWGG 3 cut(s) 32, 603, 1118
PspN4I GGNNCC 4 cut(s) 529, 610, 743, 901
PspPI GGNCC 1 cut(s) 207
PspXI VCTCGAGB 1 cut(s) 313
PsuI RGATCY 1 cut(s) 527
PvuI CGATCG 1 cut(s) 57
RsaI GTAC 1 cut(s) 1142
RsaNI GTAC 1 cut(s) 1141
RseI CAYNNNNRTG 3 cut(s) 698, 1103, 1179
SacII CCGCGG 1 cut(s) 435
SaqAI TTAA 5 cut(s) 248, 381, 1112, 1403, 1489
SatI GCNGC 1 cut(s) 1356
Sau3AI GATC 5 cut(s) 54, 333, 527, 861, 1230
Sau96I GGNCC 1 cut(s) 207
SchI GAGTC 1 cut(s) 347
ScrFI CCNGG 4 cut(s) 34, 605, 904, 1120
SfaNI GCATC 2 cut(s) 426, 819
SfoI GGCGCC 1 cut(s) 901
Sfr274I CTCGAG 1 cut(s) 313
Sfr303I CCGCGG 1 cut(s) 435
SgrBI CCGCGG 1 cut(s) 435
SinI GGWCC 1 cut(s) 207
SlaI CTCGAG 1 cut(s) 313
SmiMI CAYNNNNRTG 3 cut(s) 698, 1103, 1179
SmlI CTYRAG 5 cut(s) 313, 464, 587, 770, 1076
SmoI CTYRAG 5 cut(s) 313, 464, 587, 770, 1076
SseBI AGGCCT 1 cut(s) 1279
SsiI CCGC 2 cut(s) 432, 434
SspDI GGCGCC 1 cut(s) 899
SspMI CTAG 3 cut(s) 176, 1089, 1266
StuI AGGCCT 1 cut(s) 1279
StyD4I CCNGG 4 cut(s) 32, 603, 902, 1118
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 1 cut(s) 1181
TaqI TCGA 3 cut(s) 53, 314, 351
TfiI GAWTC 3 cut(s) 37, 583, 1133
Tru1I TTAA 5 cut(s) 248, 381, 1112, 1403, 1489
Tru9I TTAA 5 cut(s) 248, 381, 1112, 1403, 1489
TscAI CASTG 2 cut(s) 1143, 1186
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 1 cut(s) 1355
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 9 cut(s) 96, 277, 438, 530, 632, 750, 902, 927, 1163
TspGWI ACGGA 1 cut(s) 1158
TspRI CASTG 2 cut(s) 1143, 1186
Van91I CCANNNNNTGG 1 cut(s) 1245
VpaK11BI GGWCC 1 cut(s) 207
XapI RAATTY 7 cut(s) 224, 338, 347, 757, 933, 1156, 1457
XhoI CTCGAG 1 cut(s) 313
XmnI GAANNNNTTC 1 cut(s) 326
XspI CTAG 3 cut(s) 176, 1089, 1266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.