MD01G1186100.v1.1

Cleavage and polyadenylation specificity factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011019
Physical Location & Seq
Forward (+)
28483623 .. 28496081
12459 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1186100.v1.1.491

Sequence Viewer

Length: 4353 bp
ATGAGCTTCGCGGCCTACAAGATGATGCACTGGCCGACCGGCATCGAGAACTGCGCCTCGGGATTCATCTCCCACTCACGAGCTGATTTCGTGCCTCGAATGCCTCAGATCCAGACTGATGACCTGGAGTCCGACTGGCCACCTACCCGCCGAGAAATCGGCCCCATTCCGGACCTAGTCGTCACCGCTGGAAACGTCCTTGAAGTGTACGTCGTCAGAGTGCAGGAGGAAGATGGAGCCAGAGGCTCTAGGGCTTCCGGAGAACCCAAGCGTGGTGGTCTCATGGACGGCGTCTCTGGGGCTTCGCTCGAACTCGTTTGCCATTACAGAATGCACGGTAATGTGGTAACAATGGCGGTTTTATCTGGCGGAGGCGGTGATGGTTCCAGAAGAAGAGATTCTATTGTCTTAACCTTTGAAGATGCAAAAATTTCCGTTCTTGAGTTTGATGATTCAATTCATGGACTCCGTACTAGCTCTATGCACTGTTTTGAGGGCCCAGAGTGGCTTCATTTGAGAAGAGGCAGAGAGTCATTTGCAAGAGGCCCATTGGTTAAGGTTGATCCTCAAGGCAGGTGCGGAGGCGTTCTTGTTTATGGCTTTCAAATGATAATACTTAAAGCTGCACAGGGTGGCTCTGGTTTGGTTGGGGAAGATGATGGCTTTGGTTCTGGAGGTGCAATTTCTGCTCGTGTTGACTCGTCTTACATTGTTAACCTGGGAGATATGGACATGAAGCATGTAAAGGATTTTACATTTGTACATGGTTACATTGAGCCTGTGATGGTTATCCTTCACGAGCAGGAGCTTACTTGGGCTGGGCGTGTCTCATGGAAGCACCATACTTGTATGATTTCTGCACTTAGTATCAGCACAACTTTGAAGCAGCATCCGCTCATATGGTCAGCTGTTAATCTCCCCCATGATTCCTGCAAGCTGCTTGCTGTACCATCTCCAATTGGTGGTGTTCTGGTGATTAGTGCAAATTCTATCCATTATCATAGCCAGTCAGCCTCATGCGCATTGGCATTAAACAGTTATGCTGTTTCTGTTGATAGCAGTCAAGAGATGCCAAGATCTAGTTTTACTGTGGAGCTTGATGCTGCCAATGCAACTTGGTTGCTGAACGATGTAGCCTTGTTGTCTACAAAAACTGGGGAGCTACTATTGCTTACCCTTGTTTATGATGGACGGGTTGTACAGAGACTTGACCTTTCAAAGTCTAAAGCTTCAGTACTTACGTCGGGGATTACAACACTTGGAAATTCATTATTTTTTCTGGGCAGTCGGTTAGGGGACAGTTTGCTTGTGCAGTTCACTTGTGGAGGAGGGGGCTCAATGTTGTCATCTGGTTTGAAGGATGAGGTAGGGGATATTGAGGGTGATATCCCATCGGCCAAGCGACTGCGATTGTCGTCTTCGGATGCATTGCAAGATATGGTTAGCGGTGAGGAGCTCTCTCTGTTTGGGTCAGCTCCAAATAGTGCAGATTCAGCTCAGAAGTCTTTTTCATTTGCTGTAAGGGACTCTTTGATTAATGTTGGTCCTTTGAAGGACTTCTCCTATGGTTTAAGGATTAATGCAGATGCAAATGCTACTGGAATTGCTAAACAGAGTAATTACGAACTGGTCTGCTGTTCCGGTCATGGAAAGAATGGTGCCCTCTCTGTTCTTCGTCAGTCAATAAATCCAGAAATGATTACTGAGGTTGAACTACCAGGTTGTAAGGGAATTTGGACTGTTTACCACAAGAATGCACGTGGTCATAATGCTGATTCTAAAATCGCTGCTTCTGATGATGAATATCATGCATATTTAATTATAAGTCTGGAGGCTCGAACGATGGTACTTGAAACAGCAGATCTTCTGAGTGAAGTGACAGAAAGTGTTGACTATTTTGTGCAAGGGAGAACAATTGCTGCAGGGAATTTGTTTGGAAGGCGTCGAGTTGTGCAGGTCTATGAACGCGGTGCTCGAATTCTCGATGGTTCTTTTATGACTAAGGATATAAGCTTTGGAACTTTGAATGCTGAATCGGCCACCGGTTCAGAGAGCACAGTGTTATCAGTTTCTATTGTTGATCCGTATGTATTGCTGAGAATGTCTGATGGTGGTATTCGACTCCTCATTGGAGATCCTTCTTCGTGCACTGTTTCTGTAAGCATTCCAGCTGCCTTTGAGAACTTGAAGAAACAAATATCCGCTTGTACACTTTATCATGATAAAGGACCTGAGCCTTGGCTTCGGAAGGCAAGTACTGATGCTTGGCTTTCCACAGGAGTGGACGAAGCCATTGATGGTTCTGATGGTTTATTACATGATCAGGGTGATATATATTGTGTTGTTTGTTATGAAAGTGGTTCCCTTGAAATATTTGATGTGCCCAATTTTAATTGTGTTTTCTCCGTGGATAAATTTGTATCGGGAAAGACGCACCTAGTTGATACTTTAATGCAAGACCCTTCAAAAGATTCCCAAAAACTGATCAATAAAAGTTCTGAAGAAGTGAGTGGCCAGGGCAGAAAAGAAAATATAGAGGATATGAAAGTTGTAGAGCTGGCTATGCAGAGATGGTCGGGACAGCATAGCCGTCCTTTTCTTTTTGGGATATTGAATGACGGGATGATTCTTTGTTACCATGCTTACCTGTTTGAAGGTCCAGAGACTACCTCTAAAACTGAAGATTCCGCTTCGGCACAGAGTACTTCTGGCTTAACCAATGTTAGTGCTTCAAGGCTTAGAAACTTGAGATTTGCTCGTGTCCCCTTGGACACGTATGCTAGGAAGGACATGTCAACTGAAACTTCGTGCCAAAGAATGACAATTTTCAAGAACATTGCTGGTTATCAGGGGTTGTTCCTTTCTGGGTCAAGACCAGCTTGGTTAATGGTTTTCAGAGAACGTCTACGTGTTCATCCACAGCTTTGTGATGGTTCAGTTGTTGCCTTCACTGTCCTTCACAATGTGAATTGTAATCATGGGCTCATATATGTTACATCACAGGGTATATTGAAGATTTGCCAACTGCCACCTATCTCAAGCTATGACAATTATTGGCCAGTACAAAAGATTCCATTGAAAGGAACTCCTCATCAGGTCACTTACTTTGCAGAGAAGAATTTATACCCACTTATAGTTTCAGTACCTGTGCATAAACCACTGAATCAAATTCTTTCGTCTTTGGTTGATCAAGAAGCTGGCCATCAGGTTGAGAATCATAATTTGAGTTCTGATGAACTACACAGAACTTATACTGTTGATGAATTTGAGGTTCGGATTATGGAGCCAGAAAAATCTGGTGGACCTTGGCAAACTAAGGCCACCATTCCCATGCAAACTTCTGAAAATGCTCTAACTGTTCGAGTGGTTACGCTGTTTAATACAACCACCAACGAGAATGAAACACTTATAGCAATTGGGACTGCGTATGTGCAAGGGGAGGATGTTGCTGCTAGAGGACGGGTTCTTTTATTTTCAGCTGGAAACAATACTCAGAATTTGGTTTCAGAGGTGTATGCCAAAGAATTCAAGGGTGCCATATCTGCATTAGCGTCACTTCAAGGTCATTTGTTGATAGCACAAGGTCCTAAAATTACTTTAAACAAGTGGAATGGCACTGAACTGAATGGTGTGGCATTTTTTGATGTTCCACCATTGTACGTTGTGAGCTTGAATATTGTTAAGAATTTTATCCTCCTTGGTGACATTCACAAAAGCATTTACTTTCTCAGTTGGAAGGAGCAGGGATCTCAGCTCAATTTGTTGGCCAAGGATTTTGGTAACCTAGATTGTTTTGCAACGGAATTTCTGATTGATGGAAGTACACTGAGCCTTGTAGTTGCAGATGAACAAAAGAACATTCAGATCTTTTTCTATGCACCAAAGATGTCTGAGAGTTGGAAAGGTCAGAAGCTTCTCTCAAGGGCTGAATTTCATGTTGGAACCCATGTTACCAAGTTCTTGCGGTTGCAGATGCTCTCTACTTCAGGGACCAATCCAGGCTCTGACAAAACTAATCGCTATGCTTTGTTATTTGGTACCCTGGATGGCAGCATTGGTTGTGTTGCGCCTCTTGATGAACTGACATTTCGTAGGTTGCAGTCACTACAGAAAAAGCTCGTTGATGCAGTTGCCCATGTTGCTGGTTTGAATCCAAGAGCTTTCCGCCAGTTTCGGTCAAATGGAAAGGCTCATCGGCCTGGCCCTGACACCATAGTTGACTGCGAGCTTCTAACCCATTACGAGATGCTTCCATTGGAGGAACAGCTTGAAATTGCAAACCAGATAGGGACGACTCGTTCACAAATTTTCACAAACTTAAATGACCTTGCTATAGGAACGAGCTTCTTGTAA

Protein Analysis

1451

Amino Acids

158.52

Weight (kDa)

5.79

Isoelectric Point (pI)

39.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_RSE1_1st PF10433 102 - 442 4.4e-18 RSE1/DDB1/CPSF1 first beta-propeller
Beta-prop_RSE1_2nd PF23726 564 - 1009 3.4e-64 RSE1/DDB1/CPSF1 second beta-propeller
CPSF_A PF03178 1089 - 1415 1.6e-84 CPSF A subunit region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011019)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51660 AT5G51660 AT5G51660 AT5G51660
fragaria_vesca FvH4_7g28210 FvH4_7g28210 FvH4_7g28210 FvH4_7g28210
malus_domestica MD01G1186100.v1.1
prunus_persica Prupe.2G282700_v2.0.a1
pyrus_communis pycom01g19770
rosa_chinensis RchiOBHm_Chr1g0375181
rosa_laevigata RLG00000026681
rosa_multiflora Rmu_sc0000554.1_g000025
rosa_roxburghii Rroxscaffold_4G00282960
rosa_rugosa Rorug01G0389000 Rorug01G0389000
rosa_samantha Rh1AG399600 Rh1CG377800
rosa_wichuraiana Rw1G035470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1823
AarI CACCTGC 1 cut(s) 564
AasI GACNNNNNNGTC 1 cut(s) 179
Acc16I TGCGCA 1 cut(s) 1021
Acc36I ACCTGC 2 cut(s) 564, 1945
Acc65I GGTACC 1 cut(s) 4037
AccB1I GGYRCC 3 cut(s) 1658, 3533, 4037
AccB7I CCANNNNNTGG 1 cut(s) 962
AccBSI CCGCTC 1 cut(s) 895
AccI GTMKAC 2 cut(s) 1145, 2905
AccII CGCG 2 cut(s) 11, 1968
AccIII TCCGGA 2 cut(s) 169, 257
AclWI GGATC 5 cut(s) 103, 557, 2075, 2129, 3754
AcoI YGGCCR 8 cut(s) 32, 137, 1395, 2037, 2512, 3056, 3199, 3765
AcuI CTGAAG 4 cut(s) 1215, 2520, 2700, 3969
AcvI CACGTG 1 cut(s) 1760
AcyI GRCGYC 2 cut(s) 291, 1942
AdeI CACNNNGTG 2 cut(s) 632, 2965
AfiI CCNNNNNNNGG 4 cut(s) 169, 272, 962, 3080
AflIII ACRYGT 3 cut(s) 2772, 2790, 2908
AgeI ACCGGT 1 cut(s) 2042
AhdI GACNNNNNGTC 1 cut(s) 127
AjnI CCWGG 7 cut(s) 123, 717, 1717, 2514, 3997, 4041, 4198
AjuI GAANNNNNNNTTGG 2 cut(s) 3921, 3953
AleI CACNNNNGTG 1 cut(s) 2279
AloI GAACNNNNNNTCC 2 cut(s) 4282, 4314
Alw21I GWGCWC 4 cut(s) 1458, 1975, 2057, 2150
Alw26I GTCTC 5 cut(s) 284, 298, 832, 1198, 2657
Alw44I GTGCAC 1 cut(s) 2146
AlwI GGATC 5 cut(s) 103, 557, 2075, 2129, 3754
AlwNI CAGNNNCTG 2 cut(s) 3146, 4004
Ama87I CYCGRG 1 cut(s) 58
Aor13HI TCCGGA 2 cut(s) 169, 257
ApaI GGGCCC 1 cut(s) 500
ApaLI GTGCAC 1 cut(s) 2146
ApeKI GCWGC 9 cut(s) 623, 886, 937, 1103, 1787, 1919, 2171, 3449, 4050
AseI ATTAAT 2 cut(s) 1536, 1578
AsiGI ACCGGT 1 cut(s) 2042
Asp700I GAANNNNTTC 2 cut(s) 397, 1556
Asp718I GGTACC 1 cut(s) 4037
AspLEI GCGC 3 cut(s) 56, 1022, 4069
AsuHPI GGTGA 7 cut(s) 175, 389, 985, 1394, 1460, 2339, 3713
AvaI CYCGRG 1 cut(s) 58
AvaII GGWCC 7 cut(s) 172, 1544, 2228, 2657, 3302, 3584, 3990
BaeGI GKGCMC 4 cut(s) 500, 1663, 2150, 2385
BalI TGGCCA 5 cut(s) 139, 2514, 3058, 3201, 3767
BanI GGYRCC 3 cut(s) 1658, 3533, 4037
BanII GRGCYC 4 cut(s) 500, 1337, 1458, 2985
BarI GAAGNNNNNNTAC 2 cut(s) 3107, 3139
BauI CACGAG 4 cut(s) 78, 690, 797, 2757
BbrPI CACGTG 1 cut(s) 1760
BbsI GAAGAC 1 cut(s) 1410
Bbv12I GWGCWC 4 cut(s) 1458, 1975, 2057, 2150
BbvI GCAGC 9 cut(s) 610, 898, 924, 1090, 1774, 1906, 2158, 3436, 4062
BceAI ACGGC 2 cut(s) 304, 2574
BciT130I CCWGG 7 cut(s) 125, 719, 1719, 2516, 3999, 4043, 4200
BclI TGATCA 3 cut(s) 2320, 2484, 3187
BcoDI GTCTC 5 cut(s) 284, 298, 832, 1198, 2657
BfaI CTAG 8 cut(s) 176, 249, 474, 1080, 2438, 2781, 3453, 3785
BfmI CTRYAG 3 cut(s) 1920, 4106, 4332
BfuAI ACCTGC 2 cut(s) 564, 1945
BglII AGATCT 3 cut(s) 1076, 1861, 3864
BmcAI AGTACT 3 cut(s) 1236, 2257, 2704
Bme1390I CCNGG 7 cut(s) 125, 719, 1719, 2516, 3999, 4043, 4200
Bme18I GGWCC 7 cut(s) 172, 1544, 2228, 2657, 3302, 3584, 3990
BmeRI GACNNNNNGTC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 58
BmrFI CCNGG 7 cut(s) 125, 719, 1719, 2516, 3999, 4043, 4200
BmrI ACTGGG 1 cut(s) 1164
BmuI ACTGGG 1 cut(s) 1164
BpiI GAAGAC 1 cut(s) 1410
BplI GAGNNNNNCTC 6 cut(s) 1442, 1474, 2654, 2686, 2740, 2772
BpmI CTGGAG 3 cut(s) 146, 693, 1850
Bpu10I CCTNAGC 1 cut(s) 2232
BpuEI CTTGAG 5 cut(s) 461, 552, 2767, 3022, 3904
BsaAI YACGTR 3 cut(s) 1760, 2775, 2909
BsaBI GATNNNNATC 2 cut(s) 788, 1803
BsaHI GRCGYC 2 cut(s) 291, 1942
BsaI GGTCTC 1 cut(s) 284
BsaWI WCCGGW 4 cut(s) 169, 257, 1640, 2042
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 272, 962, 3080
Bse118I RCCGGY 2 cut(s) 38, 2042
Bse1I ACTGG 8 cut(s) 35, 140, 1006, 1159, 1603, 1632, 3059, 4168
Bse3DI GCAATG 2 cut(s) 1427, 2835
Bse8I GATNNNNATC 2 cut(s) 788, 1803
BseAI TCCGGA 2 cut(s) 169, 257
BseBI CCWGG 7 cut(s) 125, 719, 1719, 2516, 3999, 4043, 4200
BseGI GGATG 7 cut(s) 889, 1366, 1429, 2628, 2914, 3448, 4051
BseJI GATNNNNATC 2 cut(s) 788, 1803
BseLI CCNNNNNNNGG 4 cut(s) 169, 272, 962, 3080
BseMI GCAATG 2 cut(s) 1427, 2835
BseNI ACTGG 8 cut(s) 35, 140, 1006, 1159, 1603, 1632, 3059, 4168
BseRI GAGGAG 4 cut(s) 1341, 1466, 2114, 3078
BseSI GKGCMC 4 cut(s) 500, 1663, 2150, 2385
BseXI GCAGC 9 cut(s) 610, 898, 924, 1090, 1774, 1906, 2158, 3436, 4062
BseYI CCCAGC 1 cut(s) 818
BsgI GTGCAG 6 cut(s) 242, 609, 843, 1331, 1506, 1973
Bsh1236I CGCG 2 cut(s) 11, 1968
Bsh1285I CGRYCG 1 cut(s) 39
BshNI GGYRCC 3 cut(s) 1658, 3533, 4037
BshTI ACCGGT 1 cut(s) 2042
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 4 cut(s) 1458, 1975, 2057, 2150
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 5 cut(s) 39, 170, 258, 1641, 2043
BslFI GGGAC 7 cut(s) 1310, 1538, 2592, 2747, 3433, 4003, 4303
BslI CCNNNNNNNGG 4 cut(s) 169, 272, 962, 3080
BsmAI GTCTC 5 cut(s) 284, 298, 832, 1198, 2657
BsmBI CGTCTC 1 cut(s) 298
BsmFI GGGAC 7 cut(s) 1310, 1538, 2592, 2747, 3433, 4003, 4303
BsmI GAATGC 5 cut(s) 105, 336, 1759, 2032, 2163
Bso31I GGTCTC 1 cut(s) 284
BsoBI CYCGRG 1 cut(s) 58
Bsp120I GGGCCC 1 cut(s) 496
Bsp1286I GDGCHC 9 cut(s) 500, 1337, 1458, 1663, 1975, 2057, 2150, 2385, 2985
Bsp13I TCCGGA 2 cut(s) 169, 257
Bsp1407I TGTACA 3 cut(s) 760, 1198, 2207
BspEI TCCGGA 2 cut(s) 169, 257
BspFNI CGCG 2 cut(s) 11, 1968
BspHI TCATGA 1 cut(s) 2218
BspMAI CTGCAG 1 cut(s) 1924
BspMI ACCTGC 2 cut(s) 564, 1945
BspPI GGATC 5 cut(s) 103, 557, 2075, 2129, 3754
BspT107I GGYRCC 3 cut(s) 1658, 3533, 4037
BspTNI GGTCTC 1 cut(s) 284
BsrBI CCGCTC 1 cut(s) 895
BsrDI GCAATG 2 cut(s) 1427, 2835
BsrFI RCCGGY 2 cut(s) 38, 2042
BsrGI TGTACA 3 cut(s) 760, 1198, 2207
BsrI ACTGG 8 cut(s) 35, 140, 1006, 1159, 1603, 1632, 3059, 4168
BssAI RCCGGY 2 cut(s) 38, 2042
BssNI GRCGYC 2 cut(s) 291, 1942
BssSI CACGAG 4 cut(s) 78, 690, 797, 2757
BssT1I CCWWGG 5 cut(s) 2237, 2766, 3305, 3697, 3768
Bst2BI CACGAG 4 cut(s) 78, 690, 797, 2757
Bst2UI CCWGG 7 cut(s) 125, 719, 1719, 2516, 3999, 4043, 4200
Bst6I CTCTTC 2 cut(s) 388, 514
BstACI GRCGYC 2 cut(s) 291, 1942
BstAPI GCANNNNNTGC 1 cut(s) 686
BstAUI TGTACA 3 cut(s) 760, 1198, 2207
BstBAI YACGTR 3 cut(s) 1760, 2775, 2909
BstC8I GCNNGC 5 cut(s) 935, 942, 2559, 3199, 4226
BstDSI CCRYGG 1 cut(s) 2406
BstEII GGTNACC 1 cut(s) 3779
BstF5I GGATG 7 cut(s) 889, 1366, 1429, 2628, 2914, 3448, 4051
BstFNI CGCG 2 cut(s) 11, 1968
BstHHI GCGC 3 cut(s) 56, 1022, 4069
BstMAI GTCTC 5 cut(s) 284, 298, 832, 1198, 2657
BstMCI CGRYCG 1 cut(s) 39
BstNI CCWGG 7 cut(s) 125, 719, 1719, 2516, 3999, 4043, 4200
BstNSI RCATGY 2 cut(s) 743, 2794
BstPI GGTNACC 1 cut(s) 3779
BstSCI CCNGG 7 cut(s) 123, 717, 1717, 2514, 3997, 4041, 4198
BstSFI CTRYAG 3 cut(s) 1920, 4106, 4332
BstSLI GKGCMC 4 cut(s) 500, 1663, 2150, 2385
BstUI CGCG 2 cut(s) 11, 1968
BstV1I GCAGC 9 cut(s) 610, 898, 924, 1090, 1774, 1906, 2158, 3436, 4062
BstV2I GAAGAC 1 cut(s) 1410
BstX2I RGATCY 6 cut(s) 108, 1076, 1861, 2134, 3746, 3864
BstXI CCANNNNNNTGG 2 cut(s) 2281, 4142
BstYI RGATCY 6 cut(s) 108, 1076, 1861, 2134, 3746, 3864
BtgI CCRYGG 1 cut(s) 2406
BtsCI GGATG 7 cut(s) 889, 1366, 1429, 2628, 2914, 3448, 4051
BtsIMutI CAGTG 8 cut(s) 28, 484, 2064, 2148, 2949, 3158, 3615, 3824
BveI ACCTGC 2 cut(s) 564, 1945
Cac8I GCNNGC 5 cut(s) 935, 942, 2559, 3199, 4226
CaiI CAGNNNCTG 2 cut(s) 3146, 4004
CciI TCATGA 1 cut(s) 2218
CfoI GCGC 3 cut(s) 56, 1022, 4069
Cfr10I RCCGGY 2 cut(s) 38, 2042
CseI GACGC 4 cut(s) 280, 1931, 2440, 3540
CsiI ACCWGGT 1 cut(s) 1717
CspAI ACCGGT 1 cut(s) 2042
CspCI CAANNNNNGTGG 2 cut(s) 256, 291
DraI TTTAAA 1 cut(s) 3600
DraIII CACNNNGTG 2 cut(s) 632, 2965
DrdI GACNNNNNNGTC 1 cut(s) 179
DriI GACNNNNNGTC 1 cut(s) 127
DseDI GACNNNNNNGTC 1 cut(s) 179
EaeI YGGCCR 8 cut(s) 32, 137, 1395, 2037, 2512, 3056, 3199, 3765
Eam1104I CTCTTC 2 cut(s) 388, 514
Eam1105I GACNNNNNGTC 1 cut(s) 127
EarI CTCTTC 2 cut(s) 388, 514
EciI GGCGGA 2 cut(s) 384, 4154
Ecl136II GAGCTC 1 cut(s) 1456
Eco130I CCWWGG 5 cut(s) 2237, 2766, 3305, 3697, 3768
Eco24I GRGCYC 4 cut(s) 500, 1337, 1458, 2985
Eco31I GGTCTC 1 cut(s) 284
Eco32I GATATC 1 cut(s) 1387
Eco47I GGWCC 7 cut(s) 172, 1544, 2228, 2657, 3302, 3584, 3990
Eco53kI GAGCTC 1 cut(s) 1456
Eco57I CTGAAG 4 cut(s) 1215, 2520, 2700, 3969
Eco72I CACGTG 1 cut(s) 1760
Eco88I CYCGRG 1 cut(s) 58
Eco91I GGTNACC 1 cut(s) 3779
EcoICRI GAGCTC 1 cut(s) 1456
EcoO109I RGGNCCY 3 cut(s) 496, 2228, 3584
EcoO65I GGTNACC 1 cut(s) 3779
EcoRI GAATTC 2 cut(s) 1977, 3524
EcoRII CCWGG 7 cut(s) 123, 717, 1717, 2514, 3997, 4041, 4198
EcoRV GATATC 1 cut(s) 1387
EcoT14I CCWWGG 5 cut(s) 2237, 2766, 3305, 3697, 3768
EcoT22I ATGCAT 2 cut(s) 1429, 1813
EcoT38I GRGCYC 4 cut(s) 500, 1337, 1458, 2985
ErhI CCWWGG 5 cut(s) 2237, 2766, 3305, 3697, 3768
Esp3I CGTCTC 1 cut(s) 298
FalI AAGNNNNNCTT 4 cut(s) 1513, 1545, 2863, 2895
FaqI GGGAC 7 cut(s) 1310, 1538, 2592, 2747, 3433, 4003, 4303
FauI CCCGC 1 cut(s) 155
FauNDI CATATG 1 cut(s) 899
FbaI TGATCA 3 cut(s) 2320, 2484, 3187
FblI GTMKAC 2 cut(s) 1145, 2905
FokI GGATG 7 cut(s) 876, 1373, 1436, 2635, 2901, 3455, 4058
FriOI GRGCYC 4 cut(s) 500, 1337, 1458, 2985
FspAI RTGCGCAY 1 cut(s) 1021
FspBI CTAG 8 cut(s) 176, 249, 474, 1080, 2438, 2781, 3453, 3785
FspI TGCGCA 1 cut(s) 1021
GlaI GCGC 3 cut(s) 55, 1021, 4068
GsaI CCCAGC 1 cut(s) 822
GsuI CTGGAG 3 cut(s) 146, 693, 1850
HapII CCGG 5 cut(s) 39, 170, 258, 1641, 2043
HgaI GACGC 4 cut(s) 280, 1931, 2440, 3540
HhaI GCGC 3 cut(s) 56, 1022, 4069
Hin1I GRCGYC 2 cut(s) 291, 1942
Hin6I GCGC 3 cut(s) 54, 1020, 4067
HinP1I GCGC 3 cut(s) 54, 1020, 4067
HincII GTYRAC 5 cut(s) 697, 715, 1891, 2796, 4219
HindII GTYRAC 5 cut(s) 697, 715, 1891, 2796, 4219
HindIII AAGCTT 3 cut(s) 1227, 2011, 3911
HpaI GTTAAC 1 cut(s) 715
HpaII CCGG 5 cut(s) 39, 170, 258, 1641, 2043
HphI GGTGA 7 cut(s) 175, 389, 985, 1394, 1460, 2339, 3713
Hpy99I CGWCG 3 cut(s) 215, 1246, 1947
HpyCH4IV ACGT 8 cut(s) 195, 210, 1241, 1759, 2774, 2902, 2908, 3660
HpySE526I ACGT 8 cut(s) 195, 210, 1241, 1759, 2774, 2902, 2908, 3660
Hsp92I GRCGYC 2 cut(s) 291, 1942
HspAI GCGC 3 cut(s) 54, 1020, 4067
Kpn2I TCCGGA 2 cut(s) 169, 257
KpnI GGTACC 1 cut(s) 4041
Ksp22I TGATCA 3 cut(s) 2320, 2484, 3187
KspAI GTTAAC 1 cut(s) 715
LmnI GCTCC 8 cut(s) 236, 805, 1093, 1159, 1453, 1480, 3283, 3739
Lsp1109I GCAGC 9 cut(s) 610, 898, 924, 1090, 1774, 1906, 2158, 3436, 4062
MabI ACCWGGT 1 cut(s) 1717
MaeI CTAG 8 cut(s) 176, 249, 474, 1080, 2438, 2781, 3453, 3785
MaeII ACGT 8 cut(s) 195, 210, 1241, 1759, 2774, 2902, 2908, 3660
MbiI CCGCTC 1 cut(s) 895
MfeI CAATTG 3 cut(s) 957, 1914, 3414
MflI RGATCY 6 cut(s) 108, 1076, 1861, 2134, 3746, 3864
MhlI GDGCHC 9 cut(s) 500, 1337, 1458, 1663, 1975, 2057, 2150, 2385, 2985
MlsI TGGCCA 5 cut(s) 139, 2514, 3058, 3201, 3767
MluNI TGGCCA 5 cut(s) 139, 2514, 3058, 3201, 3767
MlyI GAGTC 7 cut(s) 137, 459, 539, 692, 1520, 2115, 4288
MmeI TCCRAC 4 cut(s) 156, 3713, 3878, 3919
Mox20I TGGCCA 5 cut(s) 139, 2514, 3058, 3201, 3767
Mph1103I ATGCAT 2 cut(s) 1429, 1813
MroI TCCGGA 2 cut(s) 169, 257
MroXI GAANNNNTTC 2 cut(s) 397, 1556
MscI TGGCCA 5 cut(s) 139, 2514, 3058, 3201, 3767
MslI CAYNNNNRTG 4 cut(s) 339, 1752, 2279, 3329
Msp20I TGGCCA 5 cut(s) 139, 2514, 3058, 3201, 3767
MspA1I CMGCKG 4 cut(s) 188, 908, 2171, 3479
MspI CCGG 5 cut(s) 39, 170, 258, 1641, 2043
MspR9I CCNGG 7 cut(s) 125, 719, 1719, 2516, 3999, 4043, 4200
MunI CAATTG 3 cut(s) 957, 1914, 3414
Mva1269I GAATGC 5 cut(s) 105, 336, 1759, 2032, 2163
MvaI CCWGG 7 cut(s) 125, 719, 1719, 2516, 3999, 4043, 4200
MvnI CGCG 2 cut(s) 11, 1968
NdeI CATATG 1 cut(s) 899
NmeAIII GCCGAG 1 cut(s) 176
NmuCI GTSAC 6 cut(s) 181, 1876, 3097, 3552, 3701, 4101
NsbI TGCGCA 1 cut(s) 1021
NsiI ATGCAT 2 cut(s) 1429, 1813
NspI RCATGY 2 cut(s) 743, 2794
OliI CACNNNNGTG 1 cut(s) 2279
PagI TCATGA 1 cut(s) 2218
PaqCI CACCTGC 1 cut(s) 564
PciI ACATGT 1 cut(s) 2790
PcsI WCGNNNNNNNCGW 1 cut(s) 1972
PctI GAATGC 5 cut(s) 105, 336, 1759, 2032, 2163
PdmI GAANNNNTTC 2 cut(s) 397, 1556
PflFI GACNNNGTC 2 cut(s) 176, 290
PflMI CCANNNNNTGG 1 cut(s) 962
PinAI ACCGGT 1 cut(s) 2042
PleI GAGTC 7 cut(s) 136, 459, 538, 692, 1520, 2115, 4288
PmaCI CACGTG 1 cut(s) 1760
PmlI CACGTG 1 cut(s) 1760
PpsI GAGTC 7 cut(s) 136, 459, 538, 692, 1520, 2115, 4288
Ppu21I YACGTR 3 cut(s) 1760, 2775, 2909
PpuMI RGGWCCY 2 cut(s) 2228, 3584
PscI ACATGT 1 cut(s) 2790
PshBI ATTAAT 2 cut(s) 1536, 1578
PsiI TTATAA 1 cut(s) 1823
Psp124BI GAGCTC 1 cut(s) 1458
Psp5II RGGWCCY 2 cut(s) 2228, 3584
Psp6I CCWGG 7 cut(s) 123, 717, 1717, 2514, 3997, 4041, 4198
PspCI CACGTG 1 cut(s) 1760
PspEI GGTNACC 1 cut(s) 3779
PspFI CCCAGC 1 cut(s) 818
PspGI CCWGG 7 cut(s) 123, 717, 1717, 2514, 3997, 4041, 4198
PspOMI GGGCCC 1 cut(s) 496
PspPPI RGGWCCY 2 cut(s) 2228, 3584
PsrI GAACNNNNNNTAC 2 cut(s) 3934, 3966
PstI CTGCAG 1 cut(s) 1924
PstNI CAGNNNCTG 2 cut(s) 3146, 4004
PsuI RGATCY 6 cut(s) 108, 1076, 1861, 2134, 3746, 3864
PsyI GACNNNGTC 2 cut(s) 176, 290
PvuII CAGCTG 3 cut(s) 908, 2171, 3479
RseI CAYNNNNRTG 4 cut(s) 339, 1752, 2279, 3329
SacI GAGCTC 1 cut(s) 1458
ScaI AGTACT 3 cut(s) 1236, 2257, 2704
SchI GAGTC 7 cut(s) 137, 459, 539, 692, 1520, 2115, 4288
ScrFI CCNGG 7 cut(s) 125, 719, 1719, 2516, 3999, 4043, 4200
SduI GDGCHC 9 cut(s) 500, 1337, 1458, 1663, 1975, 2057, 2150, 2385, 2985
SexAI ACCWGGT 1 cut(s) 1717
SfcI CTRYAG 3 cut(s) 1920, 4106, 4332
SinI GGWCC 7 cut(s) 172, 1544, 2228, 2657, 3302, 3584, 3990
SmiMI CAYNNNNRTG 4 cut(s) 339, 1752, 2279, 3329
SmlI CTYRAG 5 cut(s) 440, 567, 2746, 3037, 3919
SmoI CTYRAG 5 cut(s) 440, 567, 2746, 3037, 3919
SspI AATATT 2 cut(s) 2373, 3676
SspMI CTAG 8 cut(s) 176, 249, 474, 1080, 2438, 2781, 3453, 3785
SstI GAGCTC 1 cut(s) 1458
StyD4I CCNGG 7 cut(s) 123, 717, 1717, 2514, 3997, 4041, 4198
StyI CCWWGG 5 cut(s) 2237, 2766, 3305, 3697, 3768
TaiI ACGT 8 cut(s) 198, 213, 1244, 1762, 2777, 2905, 2911, 3663
TaqI TCGA 9 cut(s) 45, 97, 309, 1837, 1945, 1975, 1983, 2119, 3361
TaqII GACCGA 1 cut(s) 4164
TatI WGTACW 8 cut(s) 760, 1198, 1234, 2207, 2255, 2702, 3061, 3821
TauI GCSGC 1 cut(s) 14
TscAI CASTG 8 cut(s) 35, 491, 2064, 2155, 2956, 3165, 3622, 3831
TseFI GTSAC 6 cut(s) 181, 1876, 3097, 3552, 3701, 4101
TseI GCWGC 9 cut(s) 623, 886, 937, 1103, 1787, 1919, 2171, 3449, 4050
Tsp45I GTSAC 6 cut(s) 181, 1876, 3097, 3552, 3701, 4101
TspGWI ACGGA 5 cut(s) 424, 458, 2073, 2395, 3815
TspRI CASTG 8 cut(s) 35, 491, 2064, 2155, 2956, 3165, 3622, 3831
Tth111I GACNNNGTC 2 cut(s) 176, 290
Van91I CCANNNNNTGG 1 cut(s) 962
VneI GTGCAC 1 cut(s) 2146
VpaK11BI GGWCC 7 cut(s) 172, 1544, 2228, 2657, 3302, 3584, 3990
VspI ATTAAT 2 cut(s) 1536, 1578
XceI RCATGY 2 cut(s) 743, 2794
XmiI GTMKAC 2 cut(s) 1145, 2905
XmnI GAANNNNTTC 2 cut(s) 397, 1556
XspI CTAG 8 cut(s) 176, 249, 474, 1080, 2438, 2781, 3453, 3785
ZrmI AGTACT 3 cut(s) 1236, 2257, 2704
Zsp2I ATGCAT 2 cut(s) 1429, 1813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.