Cleavage and polyadenylation specificity factor
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G51660 AT5G51660 AT5G51660 AT5G51660 |
| fragaria_vesca | FvH4_7g28210 FvH4_7g28210 FvH4_7g28210 FvH4_7g28210 |
| malus_domestica | MD01G1186100.v1.1 |
| prunus_persica | Prupe.2G282700_v2.0.a1 |
| pyrus_communis | pycom01g19770 |
| rosa_chinensis | RchiOBHm_Chr1g0375181 |
| rosa_laevigata | RLG00000026681 |
| rosa_multiflora | Rmu_sc0000554.1_g000025 |
| rosa_roxburghii | Rroxscaffold_4G00282960 |
| rosa_rugosa | Rorug01G0389000 Rorug01G0389000 |
| rosa_samantha | Rh1AG399600 Rh1CG377800 |
| rosa_wichuraiana | Rw1G035470 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 936, 1814 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2580 |
| Acc65I | GGTACC | 1 cut(s) | 4031 |
| AccB1I | GGYRCC | 5 cut(s) | 239, 2009, 3515, 3923, 4031 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 965 |
| AccBSI | CCGCTC | 2 cut(s) | 805, 2875 |
| AccI | GTMKAC | 3 cut(s) | 207, 721, 1148 |
| AccII | CGCG | 6 cut(s) | 11, 146, 148, 191, 276, 3336 |
| AccIII | TCCGGA | 2 cut(s) | 260, 2238 |
| AclI | AACGTT | 1 cut(s) | 1542 |
| AclWI | GGATC | 3 cut(s) | 560, 2069, 2123 |
| AcoI | YGGCCR | 5 cut(s) | 32, 2482, 3172, 3747, 4190 |
| AcuI | CTGAAG | 5 cut(s) | 1218, 2514, 2691, 3225, 4229 |
| AcvI | CACGTG | 1 cut(s) | 1763 |
| AcyI | GRCGYC | 2 cut(s) | 240, 1933 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 965, 3053, 4327 |
| AflII | CTTAAG | 1 cut(s) | 1999 |
| AflIII | ACRYGT | 1 cut(s) | 848 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 127 |
| AhlI | ACTAGT | 1 cut(s) | 1322 |
| AjnI | CCWGG | 8 cut(s) | 729, 1720, 2316, 2705, 3066, 3991, 4035, 4192 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 3295, 3327 |
| Alw21I | GWGCWC | 6 cut(s) | 1167, 1461, 1966, 2144, 2180, 3733 |
| Alw26I | GTCTC | 5 cut(s) | 287, 720, 835, 1293, 2445 |
| Alw44I | GTGCAC | 1 cut(s) | 2140 |
| AlwI | GGATC | 3 cut(s) | 560, 2069, 2123 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 2039, 3998, 4199 |
| Ama87I | CYCGRG | 3 cut(s) | 78, 125, 4067 |
| Aor13HI | TCCGGA | 2 cut(s) | 260, 2238 |
| ApaI | GGGCCC | 1 cut(s) | 503 |
| ApaLI | GTGCAC | 1 cut(s) | 2140 |
| ApeKI | GCWGC | 6 cut(s) | 626, 680, 889, 1106, 2165, 4044 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1988, 2020 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1559 |
| Asp718I | GGTACC | 1 cut(s) | 4031 |
| AspLEI | GCGC | 7 cut(s) | 16, 56, 148, 150, 242, 305, 4063 |
| AsuC2I | CCSGG | 2 cut(s) | 1159, 2162 |
| AsuHPI | GGTGA | 7 cut(s) | 175, 392, 1168, 1397, 1463, 2333, 3695 |
| AvaI | CYCGRG | 3 cut(s) | 78, 125, 4067 |
| AvaII | GGWCC | 5 cut(s) | 161, 1547, 1740, 2222, 3566 |
| BaeGI | GKGCMC | 3 cut(s) | 503, 2144, 3928 |
| BalI | TGGCCA | 3 cut(s) | 2484, 3174, 3749 |
| BanI | GGYRCC | 5 cut(s) | 239, 2009, 3515, 3923, 4031 |
| BanII | GRGCYC | 7 cut(s) | 503, 783, 1167, 1461, 1966, 2180, 3733 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 3080, 3112 |
| BauI | CACGAG | 1 cut(s) | 693 |
| BbrPI | CACGTG | 1 cut(s) | 1763 |
| BbsI | GAAGAC | 2 cut(s) | 1773, 2249 |
| Bbv12I | GWGCWC | 6 cut(s) | 1167, 1461, 1966, 2144, 2180, 3733 |
| BbvI | GCAGC | 6 cut(s) | 613, 667, 901, 1093, 2177, 4056 |
| BceAI | ACGGC | 4 cut(s) | 135, 250, 355, 1031 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1781, 1815, 3516, 3550 |
| BciT130I | CCWGG | 8 cut(s) | 731, 1722, 2318, 2707, 3068, 3993, 4037, 4194 |
| BclI | TGATCA | 2 cut(s) | 1852, 3160 |
| BcnI | CCSGG | 2 cut(s) | 1159, 2162 |
| BcoDI | GTCTC | 5 cut(s) | 287, 720, 835, 1293, 2445 |
| BcuI | ACTAGT | 1 cut(s) | 1322 |
| BfmI | CTRYAG | 3 cut(s) | 328, 2049, 2268 |
| BfoI | RGCGCY | 2 cut(s) | 243, 306 |
| BfrI | CTTAAG | 1 cut(s) | 1999 |
| BglII | AGATCT | 2 cut(s) | 1079, 1947 |
| BlpI | GCTNAGC | 1 cut(s) | 1401 |
| BmcAI | AGTACT | 4 cut(s) | 441, 1239, 2251, 3805 |
| Bme18I | GGWCC | 5 cut(s) | 161, 1547, 1740, 2222, 3566 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 127 |
| BmeT110I | CYCGRG | 3 cut(s) | 78, 125, 4067 |
| BpiI | GAAGAC | 2 cut(s) | 1773, 2249 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 1445, 1477, 3482, 3514, 3720, 3752 |
| BpmI | CTGGAG | 1 cut(s) | 1841 |
| Bpu10I | CCTNAGC | 3 cut(s) | 630, 1574, 2226 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1401 |
| BpuEI | CTTGAG | 2 cut(s) | 555, 4283 |
| BpuMI | CCSGG | 2 cut(s) | 1159, 2162 |
| BsaAI | YACGTR | 2 cut(s) | 1763, 3643 |
| BsaHI | GRCGYC | 2 cut(s) | 240, 1933 |
| BsaI | GGTCTC | 3 cut(s) | 287, 720, 2445 |
| BsaJI | CCNNGG | 9 cut(s) | 1158, 2231, 2317, 2739, 3278, 3501, 3679, 3750, 4035 |
| BsaWI | WCCGGW | 4 cut(s) | 260, 2238, 2780, 3216 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 965, 3053, 4327 |
| Bse118I | RCCGGY | 1 cut(s) | 299 |
| Bse1I | ACTGG | 9 cut(s) | 35, 164, 1009, 1606, 1635, 3598, 3613, 3985, 4162 |
| Bse3DI | GCAATG | 3 cut(s) | 349, 1430, 2808 |
| BseAI | TCCGGA | 2 cut(s) | 260, 2238 |
| BseBI | CCWGG | 8 cut(s) | 731, 1722, 2318, 2707, 3068, 3993, 4037, 4194 |
| BseDI | CCNNGG | 9 cut(s) | 1158, 2231, 2317, 2739, 3278, 3501, 3679, 3750, 4035 |
| BseGI | GGATG | 9 cut(s) | 108, 661, 892, 1369, 2157, 2622, 2887, 3421, 4045 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 965, 3053, 4327 |
| BseMI | GCAATG | 3 cut(s) | 349, 1430, 2808 |
| BseNI | ACTGG | 9 cut(s) | 35, 164, 1009, 1606, 1635, 3598, 3613, 3985, 4162 |
| BsePI | GCGCGC | 1 cut(s) | 146 |
| BseRI | GAGGAG | 3 cut(s) | 1469, 3668, 4085 |
| BseSI | GKGCMC | 3 cut(s) | 503, 2144, 3928 |
| BseXI | GCAGC | 6 cut(s) | 613, 667, 901, 1093, 2177, 4056 |
| BseYI | CCCAGC | 1 cut(s) | 821 |
| BsgI | GTGCAG | 2 cut(s) | 1334, 1964 |
| Bsh1236I | CGCG | 6 cut(s) | 11, 146, 148, 191, 276, 3336 |
| BshNI | GGYRCC | 5 cut(s) | 239, 2009, 3515, 3923, 4031 |
| BsiHKAI | GWGCWC | 6 cut(s) | 1167, 1461, 1966, 2144, 2180, 3733 |
| BsiHKCI | CYCGRG | 3 cut(s) | 78, 125, 4067 |
| BsiSI | CCGG | 8 cut(s) | 60, 261, 300, 1158, 2162, 2239, 2781, 3217 |
| BslFI | GGGAC | 5 cut(s) | 77, 152, 3070, 3406, 4297 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 965, 3053, 4327 |
| BsmAI | GTCTC | 5 cut(s) | 287, 720, 835, 1293, 2445 |
| BsmFI | GGGAC | 5 cut(s) | 77, 152, 3070, 3406, 4297 |
| BsmI | GAATGC | 3 cut(s) | 73, 105, 1762 |
| Bso31I | GGTCTC | 3 cut(s) | 287, 720, 2445 |
| BsoBI | CYCGRG | 3 cut(s) | 78, 125, 4067 |
| Bsp120I | GGGCCC | 1 cut(s) | 499 |
| Bsp1286I | GDGCHC | 9 cut(s) | 503, 783, 1167, 1461, 1966, 2144, 2180, 3733, 3928 |
| Bsp13I | TCCGGA | 2 cut(s) | 260, 2238 |
| Bsp1407I | TGTACA | 3 cut(s) | 763, 2201, 3034 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1401 |
| Bsp19I | CCATGG | 1 cut(s) | 2231 |
| BspEI | TCCGGA | 2 cut(s) | 260, 2238 |
| BspFNI | CGCG | 6 cut(s) | 11, 146, 148, 191, 276, 3336 |
| BspHI | TCATGA | 2 cut(s) | 799, 2212 |
| BspPI | GGATC | 3 cut(s) | 560, 2069, 2123 |
| BspT107I | GGYRCC | 5 cut(s) | 239, 2009, 3515, 3923, 4031 |
| BspTI | CTTAAG | 1 cut(s) | 1999 |
| BspTNI | GGTCTC | 3 cut(s) | 287, 720, 2445 |
| BsrBI | CCGCTC | 2 cut(s) | 805, 2875 |
| BsrDI | GCAATG | 3 cut(s) | 349, 1430, 2808 |
| BsrFI | RCCGGY | 1 cut(s) | 299 |
| BsrGI | TGTACA | 3 cut(s) | 763, 2201, 3034 |
| BsrI | ACTGG | 9 cut(s) | 35, 164, 1009, 1606, 1635, 3598, 3613, 3985, 4162 |
| BssAI | RCCGGY | 1 cut(s) | 299 |
| BssECI | CCNNGG | 9 cut(s) | 1158, 2231, 2317, 2739, 3278, 3501, 3679, 3750, 4035 |
| BssHII | GCGCGC | 1 cut(s) | 146 |
| BssNI | GRCGYC | 2 cut(s) | 240, 1933 |
| BssSI | CACGAG | 1 cut(s) | 693 |
| BssT1I | CCWWGG | 5 cut(s) | 2231, 2739, 3278, 3501, 3750 |
| Bst2BI | CACGAG | 1 cut(s) | 693 |
| Bst2UI | CCWGG | 8 cut(s) | 731, 1722, 2318, 2707, 3068, 3993, 4037, 4194 |
| Bst6I | CTCTTC | 2 cut(s) | 391, 517 |
| BstACI | GRCGYC | 2 cut(s) | 240, 1933 |
| BstAFI | CTTAAG | 1 cut(s) | 1999 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 677 |
| BstAUI | TGTACA | 3 cut(s) | 763, 2201, 3034 |
| BstBAI | YACGTR | 2 cut(s) | 1763, 3643 |
| BstC8I | GCNNGC | 5 cut(s) | 141, 148, 301, 3285, 4220 |
| BstDSI | CCRYGG | 1 cut(s) | 2231 |
| BstEII | GGTNACC | 1 cut(s) | 3761 |
| BstF5I | GGATG | 9 cut(s) | 108, 661, 892, 1369, 2157, 2622, 2887, 3421, 4045 |
| BstFNI | CGCG | 6 cut(s) | 11, 146, 148, 191, 276, 3336 |
| BstH2I | RGCGCY | 2 cut(s) | 243, 306 |
| BstHHI | GCGC | 7 cut(s) | 16, 56, 148, 150, 242, 305, 4063 |
| BstMAI | GTCTC | 5 cut(s) | 287, 720, 835, 1293, 2445 |
| BstNI | CCWGG | 8 cut(s) | 731, 1722, 2318, 2707, 3068, 3993, 4037, 4194 |
| BstNSI | RCATGY | 1 cut(s) | 852 |
| BstPI | GGTNACC | 1 cut(s) | 3761 |
| BstSFI | CTRYAG | 3 cut(s) | 328, 2049, 2268 |
| BstSLI | GKGCMC | 3 cut(s) | 503, 2144, 3928 |
| BstSNI | TACGTA | 1 cut(s) | 3643 |
| BstUI | CGCG | 6 cut(s) | 11, 146, 148, 191, 276, 3336 |
| BstV1I | GCAGC | 6 cut(s) | 613, 667, 901, 1093, 2177, 4056 |
| BstV2I | GAAGAC | 2 cut(s) | 1773, 2249 |
| BstX2I | RGATCY | 3 cut(s) | 1079, 1947, 2128 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 4136 |
| BstYI | RGATCY | 3 cut(s) | 1079, 1947, 2128 |
| BtgI | CCRYGG | 1 cut(s) | 2231 |
| BtsCI | GGATG | 9 cut(s) | 108, 661, 892, 1369, 2157, 2622, 2887, 3421, 4045 |
| BtsI | GCAGTG | 1 cut(s) | 487 |
| BtsIMutI | CAGTG | 9 cut(s) | 28, 487, 1089, 2142, 2204, 2490, 2922, 3131, 3344 |
| Cac8I | GCNNGC | 5 cut(s) | 141, 148, 301, 3285, 4220 |
| CaiI | CAGNNNCTG | 3 cut(s) | 2039, 3998, 4199 |
| CciI | TCATGA | 2 cut(s) | 799, 2212 |
| CfoI | GCGC | 7 cut(s) | 16, 56, 148, 150, 242, 305, 4063 |
| Cfr10I | RCCGGY | 1 cut(s) | 299 |
| CseI | GACGC | 1 cut(s) | 1922 |
| CsiI | ACCWGGT | 2 cut(s) | 1720, 3066 |
| DinI | GGCGCC | 1 cut(s) | 241 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2580 |
| DriI | GACNNNNNGTC | 1 cut(s) | 127 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2580 |
| EaeI | YGGCCR | 5 cut(s) | 32, 2482, 3172, 3747, 4190 |
| Eam1104I | CTCTTC | 2 cut(s) | 391, 517 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 127 |
| EarI | CTCTTC | 2 cut(s) | 391, 517 |
| EciI | GGCGGA | 1 cut(s) | 4148 |
| Ecl136II | GAGCTC | 5 cut(s) | 1165, 1459, 1964, 2178, 3731 |
| Eco105I | TACGTA | 1 cut(s) | 3643 |
| Eco130I | CCWWGG | 5 cut(s) | 2231, 2739, 3278, 3501, 3750 |
| Eco24I | GRGCYC | 7 cut(s) | 503, 783, 1167, 1461, 1966, 2180, 3733 |
| Eco31I | GGTCTC | 3 cut(s) | 287, 720, 2445 |
| Eco32I | GATATC | 1 cut(s) | 3870 |
| Eco47I | GGWCC | 5 cut(s) | 161, 1547, 1740, 2222, 3566 |
| Eco53kI | GAGCTC | 5 cut(s) | 1165, 1459, 1964, 2178, 3731 |
| Eco57I | CTGAAG | 5 cut(s) | 1218, 2514, 2691, 3225, 4229 |
| Eco72I | CACGTG | 1 cut(s) | 1763 |
| Eco88I | CYCGRG | 3 cut(s) | 78, 125, 4067 |
| Eco91I | GGTNACC | 1 cut(s) | 3761 |
| EcoICRI | GAGCTC | 5 cut(s) | 1165, 1459, 1964, 2178, 3731 |
| EcoO109I | RGGNCCY | 4 cut(s) | 499, 1335, 2222, 4196 |
| EcoO65I | GGTNACC | 1 cut(s) | 3761 |
| EcoRI | GAATTC | 3 cut(s) | 1968, 3236, 3911 |
| EcoRII | CCWGG | 8 cut(s) | 729, 1720, 2316, 2705, 3066, 3991, 4035, 4192 |
| EcoRV | GATATC | 1 cut(s) | 3870 |
| EcoT14I | CCWWGG | 5 cut(s) | 2231, 2739, 3278, 3501, 3750 |
| EcoT22I | ATGCAT | 1 cut(s) | 1432 |
| EcoT38I | GRGCYC | 7 cut(s) | 503, 783, 1167, 1461, 1966, 2180, 3733 |
| EgeI | GGCGCC | 1 cut(s) | 241 |
| EheI | GGCGCC | 1 cut(s) | 241 |
| ErhI | CCWWGG | 5 cut(s) | 2231, 2739, 3278, 3501, 3750 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2836, 2868 |
| FaqI | GGGAC | 5 cut(s) | 77, 152, 3070, 3406, 4297 |
| FauI | CCCGC | 2 cut(s) | 148, 798 |
| FauNDI | CATATG | 1 cut(s) | 2749 |
| FbaI | TGATCA | 2 cut(s) | 1852, 3160 |
| FblI | GTMKAC | 3 cut(s) | 207, 721, 1148 |
| FokI | GGATG | 9 cut(s) | 95, 668, 879, 1376, 2144, 2629, 2874, 3428, 4052 |
| FriOI | GRGCYC | 7 cut(s) | 503, 783, 1167, 1461, 1966, 2180, 3733 |
| GlaI | GCGC | 7 cut(s) | 15, 55, 147, 149, 241, 304, 4062 |
| GsaI | CCCAGC | 1 cut(s) | 825 |
| GsuI | CTGGAG | 1 cut(s) | 1841 |
| HaeII | RGCGCY | 2 cut(s) | 243, 306 |
| HapII | CCGG | 8 cut(s) | 60, 261, 300, 1158, 2162, 2239, 2781, 3217 |
| HgaI | GACGC | 1 cut(s) | 1922 |
| HhaI | GCGC | 7 cut(s) | 16, 56, 148, 150, 242, 305, 4063 |
| Hin1I | GRCGYC | 2 cut(s) | 240, 1933 |
| Hin6I | GCGC | 7 cut(s) | 14, 54, 146, 148, 240, 303, 4061 |
| HinP1I | GCGC | 7 cut(s) | 14, 54, 146, 148, 240, 303, 4061 |
| HincII | GTYRAC | 5 cut(s) | 1540, 1882, 2437, 2941, 4213 |
| HindII | GTYRAC | 5 cut(s) | 1540, 1882, 2437, 2941, 4213 |
| HindIII | AAGCTT | 4 cut(s) | 1230, 2002, 3893, 4109 |
| HpaI | GTTAAC | 2 cut(s) | 1540, 2941 |
| HpaII | CCGG | 8 cut(s) | 60, 261, 300, 1158, 2162, 2239, 2781, 3217 |
| HphI | GGTGA | 7 cut(s) | 175, 392, 1168, 1397, 1463, 2333, 3695 |
| Hpy99I | CGWCG | 6 cut(s) | 40, 122, 182, 215, 1249, 1938 |
| HpyCH4IV | ACGT | 7 cut(s) | 195, 210, 1244, 1542, 1762, 2373, 3642 |
| HpySE526I | ACGT | 7 cut(s) | 195, 210, 1244, 1542, 1762, 2373, 3642 |
| Hsp92I | GRCGYC | 2 cut(s) | 240, 1933 |
| HspAI | GCGC | 7 cut(s) | 14, 54, 146, 148, 240, 303, 4061 |
| KasI | GGCGCC | 1 cut(s) | 239 |
| Kpn2I | TCCGGA | 2 cut(s) | 260, 2238 |
| KpnI | GGTACC | 1 cut(s) | 4035 |
| KroI | GCCGGC | 1 cut(s) | 299 |
| KroNI | GCCGGC | 1 cut(s) | 301 |
| Ksp22I | TGATCA | 2 cut(s) | 1852, 3160 |
| KspAI | GTTAAC | 2 cut(s) | 1540, 2941 |
| Lsp1109I | GCAGC | 6 cut(s) | 613, 667, 901, 1093, 2177, 4056 |
| MabI | ACCWGGT | 2 cut(s) | 1720, 3066 |
| MaeII | ACGT | 7 cut(s) | 195, 210, 1244, 1542, 1762, 2373, 3642 |
| MbiI | CCGCTC | 2 cut(s) | 805, 2875 |
| MfeI | CAATTG | 4 cut(s) | 960, 1905, 2910, 3387 |
| MflI | RGATCY | 3 cut(s) | 1079, 1947, 2128 |
| MhlI | GDGCHC | 9 cut(s) | 503, 783, 1167, 1461, 1966, 2144, 2180, 3733, 3928 |
| MlsI | TGGCCA | 3 cut(s) | 2484, 3174, 3749 |
| MluNI | TGGCCA | 3 cut(s) | 2484, 3174, 3749 |
| Mly113I | GGCGCC | 1 cut(s) | 240 |
| MlyI | GAGTC | 8 cut(s) | 137, 710, 1787, 1984, 2756, 2949, 3052, 3208 |
| MmeI | TCCRAC | 5 cut(s) | 1243, 2104, 3695, 3860, 4230 |
| Mox20I | TGGCCA | 3 cut(s) | 2484, 3174, 3749 |
| Mph1103I | ATGCAT | 1 cut(s) | 1432 |
| MreI | CGCCGGCG | 1 cut(s) | 299 |
| MroI | TCCGGA | 2 cut(s) | 260, 2238 |
| MroNI | GCCGGC | 1 cut(s) | 299 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1559 |
| MscI | TGGCCA | 3 cut(s) | 2484, 3174, 3749 |
| MslI | CAYNNNNRTG | 5 cut(s) | 342, 1755, 2444, 3302, 3921 |
| Msp20I | TGGCCA | 3 cut(s) | 2484, 3174, 3749 |
| MspA1I | CMGCKG | 2 cut(s) | 1352, 1850 |
| MspCI | CTTAAG | 1 cut(s) | 1999 |
| MspI | CCGG | 8 cut(s) | 60, 261, 300, 1158, 2162, 2239, 2781, 3217 |
| MunI | CAATTG | 4 cut(s) | 960, 1905, 2910, 3387 |
| Mva1269I | GAATGC | 3 cut(s) | 73, 105, 1762 |
| MvaI | CCWGG | 8 cut(s) | 731, 1722, 2318, 2707, 3068, 3993, 4037, 4194 |
| MvnI | CGCG | 6 cut(s) | 11, 146, 148, 191, 276, 3336 |
| NaeI | GCCGGC | 1 cut(s) | 301 |
| NarI | GGCGCC | 1 cut(s) | 240 |
| NciI | CCSGG | 2 cut(s) | 1159, 2162 |
| NcoI | CCATGG | 1 cut(s) | 2231 |
| NdeI | CATATG | 1 cut(s) | 2749 |
| NgoMIV | GCCGGC | 1 cut(s) | 299 |
| NmeAIII | GCCGAG | 1 cut(s) | 3109 |
| NmuCI | GTSAC | 5 cut(s) | 181, 1867, 3070, 3683, 3931 |
| NsiI | ATGCAT | 1 cut(s) | 1432 |
| NspI | RCATGY | 1 cut(s) | 852 |
| PaeR7I | CTCGAG | 3 cut(s) | 78, 125, 4067 |
| PagI | TCATGA | 2 cut(s) | 799, 2212 |
| PauI | GCGCGC | 1 cut(s) | 146 |
| PciI | ACATGT | 1 cut(s) | 848 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2343 |
| PctI | GAATGC | 3 cut(s) | 73, 105, 1762 |
| PdiI | GCCGGC | 1 cut(s) | 301 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1559 |
| PflFI | GACNNNGTC | 2 cut(s) | 89, 293 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 965 |
| PleI | GAGTC | 8 cut(s) | 136, 709, 1786, 1984, 2756, 2949, 3052, 3207 |
| PluTI | GGCGCC | 1 cut(s) | 243 |
| PmaCI | CACGTG | 1 cut(s) | 1763 |
| PmlI | CACGTG | 1 cut(s) | 1763 |
| PpsI | GAGTC | 8 cut(s) | 136, 709, 1786, 1984, 2756, 2949, 3052, 3207 |
| Ppu21I | YACGTR | 2 cut(s) | 1763, 3643 |
| PpuMI | RGGWCCY | 1 cut(s) | 2222 |
| PscI | ACATGT | 1 cut(s) | 848 |
| PsiI | TTATAA | 2 cut(s) | 936, 1814 |
| Psp124BI | GAGCTC | 5 cut(s) | 1167, 1461, 1966, 2180, 3733 |
| Psp1406I | AACGTT | 1 cut(s) | 1542 |
| Psp5II | RGGWCCY | 1 cut(s) | 2222 |
| Psp6I | CCWGG | 8 cut(s) | 729, 1720, 2316, 2705, 3066, 3991, 4035, 4192 |
| PspCI | CACGTG | 1 cut(s) | 1763 |
| PspEI | GGTNACC | 1 cut(s) | 3761 |
| PspFI | CCCAGC | 1 cut(s) | 821 |
| PspGI | CCWGG | 8 cut(s) | 729, 1720, 2316, 2705, 3066, 3991, 4035, 4192 |
| PspOMI | GGGCCC | 1 cut(s) | 499 |
| PspPPI | RGGWCCY | 1 cut(s) | 2222 |
| PspXI | VCTCGAGB | 1 cut(s) | 125 |
| PstNI | CAGNNNCTG | 3 cut(s) | 2039, 3998, 4199 |
| PsuI | RGATCY | 3 cut(s) | 1079, 1947, 2128 |
| PsyI | GACNNNGTC | 2 cut(s) | 89, 293 |
| PteI | GCGCGC | 1 cut(s) | 146 |
| PvuII | CAGCTG | 2 cut(s) | 1352, 1850 |
| RseI | CAYNNNNRTG | 5 cut(s) | 342, 1755, 2444, 3302, 3921 |
| SacI | GAGCTC | 5 cut(s) | 1167, 1461, 1966, 2180, 3733 |
| ScaI | AGTACT | 4 cut(s) | 441, 1239, 2251, 3805 |
| SchI | GAGTC | 8 cut(s) | 137, 710, 1787, 1984, 2756, 2949, 3052, 3208 |
| SduI | GDGCHC | 9 cut(s) | 503, 783, 1167, 1461, 1966, 2144, 2180, 3733, 3928 |
| SexAI | ACCWGGT | 2 cut(s) | 1720, 3066 |
| SfcI | CTRYAG | 3 cut(s) | 328, 2049, 2268 |
| SfoI | GGCGCC | 1 cut(s) | 241 |
| Sfr274I | CTCGAG | 3 cut(s) | 78, 125, 4067 |
| SgrAI | CRCCGGYG | 1 cut(s) | 299 |
| SinI | GGWCC | 5 cut(s) | 161, 1547, 1740, 2222, 3566 |
| SlaI | CTCGAG | 3 cut(s) | 78, 125, 4067 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 342, 1755, 2444, 3302, 3921 |
| SmlI | CTYRAG | 6 cut(s) | 78, 125, 570, 1999, 4067, 4262 |
| SmoI | CTYRAG | 6 cut(s) | 78, 125, 570, 1999, 4067, 4262 |
| SnaBI | TACGTA | 1 cut(s) | 3643 |
| SpeI | ACTAGT | 1 cut(s) | 1322 |
| SspDI | GGCGCC | 1 cut(s) | 239 |
| SspI | AATATT | 3 cut(s) | 2367, 2604, 3658 |
| SstI | GAGCTC | 5 cut(s) | 1167, 1461, 1966, 2180, 3733 |
| StyI | CCWWGG | 5 cut(s) | 2231, 2739, 3278, 3501, 3750 |
| TaiI | ACGT | 7 cut(s) | 198, 213, 1247, 1545, 1765, 2376, 3645 |
| TaqII | GACCGA | 1 cut(s) | 131 |
| TatI | WGTACW | 7 cut(s) | 439, 763, 1237, 2201, 2249, 3034, 3803 |
| TauI | GCSGC | 8 cut(s) | 14, 146, 191, 240, 279, 1913, 2355, 2878 |
| TscAI | CASTG | 9 cut(s) | 35, 494, 1096, 2149, 2211, 2497, 2929, 3138, 3351 |
| TseFI | GTSAC | 5 cut(s) | 181, 1867, 3070, 3683, 3931 |
| TseI | GCWGC | 6 cut(s) | 626, 680, 889, 1106, 2165, 4044 |
| Tsp45I | GTSAC | 5 cut(s) | 181, 1867, 3070, 3683, 3931 |
| TspGWI | ACGGA | 3 cut(s) | 2067, 3036, 3103 |
| TspRI | CASTG | 9 cut(s) | 35, 494, 1096, 2149, 2211, 2497, 2929, 3138, 3351 |
| Tth111I | GACNNNGTC | 2 cut(s) | 89, 293 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 965 |
| Vha464I | CTTAAG | 1 cut(s) | 1999 |
| VneI | GTGCAC | 1 cut(s) | 2140 |
| VpaK11BI | GGWCC | 5 cut(s) | 161, 1547, 1740, 2222, 3566 |
| XbaI | TCTAGA | 2 cut(s) | 251, 1972 |
| XceI | RCATGY | 1 cut(s) | 852 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 2477, 3526 |
| XhoI | CTCGAG | 3 cut(s) | 78, 125, 4067 |
| XmiI | GTMKAC | 3 cut(s) | 207, 721, 1148 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1559 |
| ZrmI | AGTACT | 4 cut(s) | 441, 1239, 2251, 3805 |
| Zsp2I | ATGCAT | 1 cut(s) | 1432 |