MD01G1196900.v1.1

BAG family molecular chaperone regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006122
Physical Location & Seq
Reverse (-)
29500220 .. 29502393
2174 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1196900.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGATGAGGATGAAGACGAAGACTACAGGGGTGTCACTGGCCGAGTTGAATGGAAGTTCGGGCGGCGGAGGAGCTGAGTCGGGGCAGGGAGAGTGGGAGCTCAGACCGGGAGGAATGCTGGTTCAGAAGCGAAATCCGGACTCTGATCGGAACTCGGCGCCTCCACCGACAATTAGAGTTCGGGTCAAATATGGGTCCATTTACCATGAAATGAGCATCAGTGCTCAGTCTTCTTTTGGGGATTTGAAGAAAATGTTGGTTGGACCGACAGGTTTGCACCATGAAGATCAGAAATTGATATTCAAAGACAAGGAGAGGGACTCGAAGGCGTTTCTTGACATGTCCGGGGTGAAGGACCGGTCGAAAATGGTGTTAGTTGAGGACCCTATTAGCCAAGAAAAGCGATATCTTGAGATGCGACGCAATGCAAAGATGGAGAAGGCTTCGAAATCGATTTCTGAAATCAGCTTGGAAGTAGACAGGCTTGCCGGCCAGGTGTCGGCTCTTGAGTCAATAATCACGAAAGGTAAAAAAGTTGCAGAGCAAGATGTGCTTGTTTTAATTGAGCAGTTGATGAACCAGTTGCTTAAATTAGATGGAATCATGGGCGACGGAGATGTGAAACTGCAGAGGAAGATGCAGGTGAAACGCGTGCAGAAGTATGTCGAAACTCTAGATGTATTGAAAGCTAAGAACTCCTCGCCTAGCAGCAATGGAAGTCAGATTCCAAAGCAGGTTCAGCAGAGGCATTCAGATGGTCATTCCAATGGGAATGGACATAGACTGGCACCAATTCAAGAGCATCAACCGAGGAATTCGGTTGACTATCCACCTACACATAATCAGCAAGTGCAACAGCAAGAACAACCATCAAGGCATTCGGCATCAGGGCCGGTAGTTGTGACCACACAGTGGGAGACATTCGATTCTGCCCCGGCATTGATCCCAACCTCTTCAACATCCAAGGCCGCCAATCAATCTCCGTACCCTAAGTTTAACTGGGAATCGTTCGAGTAA

Protein Analysis

339

Amino Acids

37.53

Weight (kDa)

9.31

Isoelectric Point (pI)

48.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAG PF02179 152 - 227 1.9e-17 BAG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 631
Acc36I ACCTGC 2 cut(s) 631, 724
AccB1I GGYRCC 2 cut(s) 157, 787
AccB7I CCANNNNNTGG 1 cut(s) 912
AccI GTMKAC 1 cut(s) 477
AccII CGCG 1 cut(s) 651
AccIII TCCGGA 1 cut(s) 136
AciI CCGC 3 cut(s) 63, 66, 969
AclWI GGATC 1 cut(s) 937
AcoI YGGCCR 2 cut(s) 39, 490
AcsI RAATTY 1 cut(s) 814
AcyI GRCGYC 1 cut(s) 158
AdeI CACNNNGTG 1 cut(s) 912
AfaI GTAC 1 cut(s) 986
AfiI CCNNNNNNNGG 2 cut(s) 499, 912
AflIII ACRYGT 2 cut(s) 339, 649
AgeI ACCGGT 1 cut(s) 357
AgsI TTSAA 6 cut(s) 49, 247, 304, 685, 797, 957
AjnI CCWGG 1 cut(s) 492
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
AloI GAACNNNNNNTCC 2 cut(s) 105, 137
AluBI AGCT 4 cut(s) 74, 100, 468, 689
AluI AGCT 4 cut(s) 74, 100, 468, 689
Alw21I GWGCWC 2 cut(s) 102, 226
Alw26I GTCTC 1 cut(s) 911
AlwI GGATC 1 cut(s) 937
Aor13HI TCCGGA 1 cut(s) 136
AoxI GGCC 4 cut(s) 39, 490, 890, 966
ApeKI GCWGC 1 cut(s) 708
ApoI RAATTY 1 cut(s) 814
AsiGI ACCGGT 1 cut(s) 357
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 5 cut(s) 195, 263, 355, 382, 890
AsuC2I CCSGG 3 cut(s) 108, 346, 935
AsuHPI GGTGA 2 cut(s) 361, 655
AsuII TTCGAA 1 cut(s) 446
AvaII GGWCC 4 cut(s) 195, 263, 355, 382
BanI GGYRCC 2 cut(s) 157, 787
BanII GRGCYC 1 cut(s) 102
BbsI GAAGAC 3 cut(s) 20, 26, 222
Bbv12I GWGCWC 2 cut(s) 102, 226
BbvI GCAGC 1 cut(s) 720
BccI CCATC 4 cut(s) 427, 590, 749, 877
BcgI CGANNNNNNTGC 2 cut(s) 256, 290
BciT130I CCWGG 1 cut(s) 494
BcnI CCSGG 3 cut(s) 108, 346, 935
BcoDI GTCTC 1 cut(s) 911
BfaI CTAG 2 cut(s) 674, 705
BfmI CTRYAG 2 cut(s) 24, 626
BfoI RGCGCY 1 cut(s) 161
BfuAI ACCTGC 2 cut(s) 631, 724
BisI GCNGC 3 cut(s) 64, 709, 969
BlsI GCNGC 3 cut(s) 65, 710, 970
Bme1390I CCNGG 4 cut(s) 108, 346, 494, 935
Bme18I GGWCC 4 cut(s) 195, 263, 355, 382
BmgT120I GGNCC 5 cut(s) 195, 263, 355, 382, 890
BmiI GGNNCC 4 cut(s) 159, 196, 384, 789
BmrFI CCNGG 4 cut(s) 108, 346, 494, 935
BmrI ACTGGG 1 cut(s) 1009
BmsI GCATC 5 cut(s) 225, 405, 627, 811, 893
BmuI ACTGGG 1 cut(s) 1009
BpiI GAAGAC 3 cut(s) 20, 26, 222
BplI GAGNNNNNCTC 2 cut(s) 305, 337
Bpu14I TTCGAA 1 cut(s) 446
BpuEI CTTGAG 2 cut(s) 431, 527
BpuMI CCSGG 3 cut(s) 108, 346, 935
Bsa29I ATCGAT 1 cut(s) 452
BsaHI GRCGYC 1 cut(s) 158
BsaJI CCNNGG 4 cut(s) 345, 809, 933, 963
BsaWI WCCGGW 2 cut(s) 136, 357
Bsc4I CCNNNNNNNGG 2 cut(s) 499, 912
Bse118I RCCGGY 3 cut(s) 357, 488, 892
Bse1I ACTGG 4 cut(s) 42, 580, 789, 1004
Bse3DI GCAATG 2 cut(s) 430, 718
BseAI TCCGGA 1 cut(s) 136
BseBI CCWGG 1 cut(s) 494
BseCI ATCGAT 1 cut(s) 452
BseDI CCNNGG 4 cut(s) 345, 809, 933, 963
BseGI GGATG 2 cut(s) 15, 959
BseLI CCNNNNNNNGG 2 cut(s) 499, 912
BseMI GCAATG 2 cut(s) 430, 718
BseMII CTCAG 3 cut(s) 66, 115, 239
BseNI ACTGG 4 cut(s) 42, 580, 789, 1004
BseRI GAGGAG 2 cut(s) 84, 688
BseXI GCAGC 1 cut(s) 720
BsgI GTGCAG 1 cut(s) 674
Bsh1236I CGCG 1 cut(s) 651
Bsh1285I CGRYCG 1 cut(s) 362
BshFI GGCC 4 cut(s) 41, 492, 892, 968
BshNI GGYRCC 2 cut(s) 157, 787
BshTI ACCGGT 1 cut(s) 357
BshVI ATCGAT 1 cut(s) 452
BsiEI CGRYCG 1 cut(s) 362
BsiHKAI GWGCWC 2 cut(s) 102, 226
BsiSI CCGG 7 cut(s) 107, 137, 345, 358, 489, 893, 935
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 499, 912
BsmAI GTCTC 1 cut(s) 911
BsmFI GGGAC 1 cut(s) 332
BsmI GAATGC 3 cut(s) 120, 748, 877
BsnI GGCC 4 cut(s) 41, 492, 892, 968
Bsp119I TTCGAA 1 cut(s) 446
Bsp1286I GDGCHC 2 cut(s) 102, 226
Bsp13I TCCGGA 1 cut(s) 136
Bsp143I GATC 3 cut(s) 145, 286, 942
BspACI CCGC 3 cut(s) 63, 66, 969
BspANI GGCC 4 cut(s) 41, 492, 892, 968
BspCNI CTCAG 3 cut(s) 67, 114, 238
BspDI ATCGAT 1 cut(s) 452
BspEI TCCGGA 1 cut(s) 136
BspFNI CGCG 1 cut(s) 651
BspLI GGNNCC 4 cut(s) 159, 196, 384, 789
BspMAI CTGCAG 1 cut(s) 630
BspMI ACCTGC 2 cut(s) 631, 724
BspPI GGATC 1 cut(s) 937
BspT104I TTCGAA 1 cut(s) 446
BspT107I GGYRCC 2 cut(s) 157, 787
BsrDI GCAATG 2 cut(s) 430, 718
BsrFI RCCGGY 3 cut(s) 357, 488, 892
BsrI ACTGG 4 cut(s) 42, 580, 789, 1004
BssAI RCCGGY 3 cut(s) 357, 488, 892
BssECI CCNNGG 4 cut(s) 345, 809, 933, 963
BssMI GATC 3 cut(s) 145, 286, 942
BssNI GRCGYC 1 cut(s) 158
BssT1I CCWWGG 1 cut(s) 963
Bst2UI CCWGG 1 cut(s) 494
Bst4CI ACNGT 1 cut(s) 912
Bst6I CTCTTC 1 cut(s) 958
BstACI GRCGYC 1 cut(s) 158
BstAPI GCANNNNNTGC 1 cut(s) 550
BstBI TTCGAA 1 cut(s) 446
BstC8I GCNNGC 3 cut(s) 486, 490, 653
BstDEI CTNAG 5 cut(s) 75, 101, 225, 690, 990
BstF5I GGATG 2 cut(s) 15, 959
BstFNI CGCG 1 cut(s) 651
BstH2I RGCGCY 1 cut(s) 161
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 3 cut(s) 148, 289, 945
BstMAI GTCTC 1 cut(s) 911
BstMBI GATC 3 cut(s) 145, 286, 942
BstMCI CGRYCG 1 cut(s) 362
BstMWI GCNNNNNNNGC 2 cut(s) 550, 739
BstNI CCWGG 1 cut(s) 494
BstNSI RCATGY 1 cut(s) 343
BstSCI CCNGG 4 cut(s) 106, 344, 492, 933
BstSFI CTRYAG 2 cut(s) 24, 626
BstUI CGCG 1 cut(s) 651
BstV1I GCAGC 1 cut(s) 720
BstV2I GAAGAC 3 cut(s) 20, 26, 222
Bsu15I ATCGAT 1 cut(s) 452
BsuRI GGCC 4 cut(s) 41, 492, 892, 968
BsuTUI ATCGAT 1 cut(s) 452
BtsCI GGATG 2 cut(s) 15, 959
BtsIMutI CAGTG 3 cut(s) 35, 226, 917
BveI ACCTGC 2 cut(s) 631, 724
Cac8I GCNNGC 3 cut(s) 486, 490, 653
CfoI GCGC 1 cut(s) 160
Cfr10I RCCGGY 3 cut(s) 357, 488, 892
Cfr13I GGNCC 5 cut(s) 195, 263, 355, 382, 890
ClaI ATCGAT 1 cut(s) 452
CseI GACGC 1 cut(s) 429
Csp6I GTAC 1 cut(s) 985
CspAI ACCGGT 1 cut(s) 357
CviAII CATG 4 cut(s) 206, 281, 340, 604
CviQI GTAC 1 cut(s) 985
DdeI CTNAG 5 cut(s) 75, 101, 225, 690, 990
DinI GGCGCC 1 cut(s) 159
DpnI GATC 3 cut(s) 147, 288, 944
DpnII GATC 3 cut(s) 145, 286, 942
DraIII CACNNNGTG 1 cut(s) 912
EaeI YGGCCR 2 cut(s) 39, 490
Eam1104I CTCTTC 1 cut(s) 958
EarI CTCTTC 1 cut(s) 958
EciI GGCGGA 1 cut(s) 81
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 963
Eco24I GRGCYC 1 cut(s) 102
Eco32I GATATC 1 cut(s) 407
Eco47I GGWCC 4 cut(s) 195, 263, 355, 382
Eco53kI GAGCTC 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 100
EcoO109I RGGNCCY 1 cut(s) 382
EcoRI GAATTC 1 cut(s) 814
EcoRII CCWGG 1 cut(s) 492
EcoRV GATATC 1 cut(s) 407
EcoT14I CCWWGG 1 cut(s) 963
EcoT38I GRGCYC 1 cut(s) 102
EgeI GGCGCC 1 cut(s) 159
EheI GGCGCC 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 963
FaeI CATG 4 cut(s) 209, 284, 343, 607
FaiI YATR 8 cut(s) 192, 207, 282, 341, 605, 663, 780, 840
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FaqI GGGAC 1 cut(s) 332
FatI CATG 4 cut(s) 205, 280, 339, 603
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 3 cut(s) 64, 709, 969
FokI GGATG 2 cut(s) 22, 946
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 3 cut(s) 64, 709, 969
FspBI CTAG 2 cut(s) 674, 705
GlaI GCGC 1 cut(s) 159
GluI GCNGC 3 cut(s) 64, 709, 969
HaeII RGCGCY 1 cut(s) 161
HaeIII GGCC 4 cut(s) 41, 492, 892, 968
HapII CCGG 7 cut(s) 107, 137, 345, 358, 489, 893, 935
HgaI GACGC 1 cut(s) 429
HhaI GCGC 1 cut(s) 160
Hin1I GRCGYC 1 cut(s) 158
Hin1II CATG 4 cut(s) 209, 284, 343, 607
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HinfI GANTC 8 cut(s) 77, 140, 320, 509, 600, 724, 926, 1004
HpaII CCGG 7 cut(s) 107, 137, 345, 358, 489, 893, 935
HphI GGTGA 2 cut(s) 361, 655
Hpy166II GTNNAC 2 cut(s) 478, 823
Hpy188I TCNGA 8 cut(s) 104, 126, 145, 150, 291, 460, 723, 754
Hpy188III TCNNGA 7 cut(s) 137, 335, 410, 506, 520, 674, 797
Hpy8I GTNNAC 2 cut(s) 478, 823
Hpy99I CGWCG 2 cut(s) 423, 614
HpyAV CCTTC 3 cut(s) 319, 346, 433
HpyCH4III ACNGT 1 cut(s) 912
HpyCH4V TGCA 7 cut(s) 277, 428, 539, 628, 640, 655, 853
HpyF10VI GCNNNNNNNGC 2 cut(s) 550, 739
HpyF3I CTNAG 5 cut(s) 75, 101, 225, 690, 990
Hsp92I GRCGYC 1 cut(s) 158
Hsp92II CATG 4 cut(s) 209, 284, 343, 607
HspAI GCGC 1 cut(s) 158
KasI GGCGCC 1 cut(s) 157
Kpn2I TCCGGA 1 cut(s) 136
KroI GCCGGC 1 cut(s) 488
KroNI GCCGGC 1 cut(s) 490
Kzo9I GATC 3 cut(s) 145, 286, 942
LmnI GCTCC 2 cut(s) 71, 97
Lsp1109I GCAGC 1 cut(s) 720
LweI GCATC 5 cut(s) 225, 405, 627, 811, 893
MaeI CTAG 2 cut(s) 674, 705
MaeIII GTNAC 2 cut(s) 33, 901
MalI GATC 3 cut(s) 147, 288, 944
MboI GATC 3 cut(s) 145, 286, 942
MboII GAAGA 7 cut(s) 25, 31, 222, 259, 296, 646, 945
MhlI GDGCHC 2 cut(s) 102, 226
MluCI AATT 6 cut(s) 171, 293, 561, 590, 792, 814
MluI ACGCGT 1 cut(s) 649
Mly113I GGCGCC 1 cut(s) 158
MlyI GAGTC 4 cut(s) 86, 134, 314, 518
MmeI TCCRAC 1 cut(s) 241
MroI TCCGGA 1 cut(s) 136
MroNI GCCGGC 1 cut(s) 488
MseI TTAA 3 cut(s) 560, 588, 996
MslI CAYNNNNRTG 2 cut(s) 753, 765
MspI CCGG 7 cut(s) 107, 137, 345, 358, 489, 893, 935
MspR9I CCNGG 4 cut(s) 108, 346, 494, 935
Mva1269I GAATGC 3 cut(s) 120, 748, 877
MvaI CCWGG 1 cut(s) 494
MvnI CGCG 1 cut(s) 651
MwoI GCNNNNNNNGC 2 cut(s) 550, 739
NaeI GCCGGC 1 cut(s) 490
NarI GGCGCC 1 cut(s) 158
NciI CCSGG 3 cut(s) 108, 346, 935
NdeII GATC 3 cut(s) 145, 286, 942
NgoMIV GCCGGC 1 cut(s) 488
NlaIII CATG 4 cut(s) 209, 284, 343, 607
NlaIV GGNNCC 4 cut(s) 159, 196, 384, 789
NmeAIII GCCGAG 2 cut(s) 67, 134
NmuCI GTSAC 2 cut(s) 33, 901
NspI RCATGY 1 cut(s) 343
NspV TTCGAA 1 cut(s) 446
PaqCI CACCTGC 1 cut(s) 631
PciI ACATGT 1 cut(s) 339
PctI GAATGC 3 cut(s) 120, 748, 877
PdiI GCCGGC 1 cut(s) 490
PfeI GAWTC 4 cut(s) 600, 724, 926, 1004
PflMI CCANNNNNTGG 1 cut(s) 912
PinAI ACCGGT 1 cut(s) 357
PkrI GCNGC 3 cut(s) 65, 710, 970
PleI GAGTC 4 cut(s) 85, 134, 314, 517
PluTI GGCGCC 1 cut(s) 161
PpsI GAGTC 4 cut(s) 85, 134, 314, 517
PpuMI RGGWCCY 1 cut(s) 382
PscI ACATGT 1 cut(s) 339
Psp124BI GAGCTC 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 492
PspGI CCWGG 1 cut(s) 492
PspN4I GGNNCC 4 cut(s) 159, 196, 384, 789
PspPI GGNCC 5 cut(s) 195, 263, 355, 382, 890
PspPPI RGGWCCY 1 cut(s) 382
PstI CTGCAG 1 cut(s) 630
RsaI GTAC 1 cut(s) 986
RsaNI GTAC 1 cut(s) 985
RseI CAYNNNNRTG 2 cut(s) 753, 765
SacI GAGCTC 1 cut(s) 102
SaqAI TTAA 3 cut(s) 560, 588, 996
SatI GCNGC 3 cut(s) 64, 709, 969
Sau3AI GATC 3 cut(s) 145, 286, 942
Sau96I GGNCC 5 cut(s) 195, 263, 355, 382, 890
SchI GAGTC 4 cut(s) 86, 134, 314, 518
ScrFI CCNGG 4 cut(s) 108, 346, 494, 935
SduI GDGCHC 2 cut(s) 102, 226
SfaNI GCATC 5 cut(s) 225, 405, 627, 811, 893
SfcI CTRYAG 2 cut(s) 24, 626
SfoI GGCGCC 1 cut(s) 159
SfuI TTCGAA 1 cut(s) 446
SinI GGWCC 4 cut(s) 195, 263, 355, 382
SmiMI CAYNNNNRTG 2 cut(s) 753, 765
SmlI CTYRAG 2 cut(s) 410, 506
SmoI CTYRAG 2 cut(s) 410, 506
Sse9I AATT 6 cut(s) 171, 293, 561, 590, 792, 814
SsiI CCGC 3 cut(s) 63, 66, 969
SspDI GGCGCC 1 cut(s) 157
SspMI CTAG 2 cut(s) 674, 705
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 4 cut(s) 106, 344, 492, 933
StyI CCWWGG 1 cut(s) 963
TaaI ACNGT 1 cut(s) 912
TaqI TCGA 7 cut(s) 323, 362, 446, 452, 666, 924, 1011
TaqII GACCGA 1 cut(s) 280
TasI AATT 6 cut(s) 171, 293, 561, 590, 792, 814
TauI GCSGC 2 cut(s) 66, 971
TfiI GAWTC 4 cut(s) 600, 724, 926, 1004
Tru1I TTAA 3 cut(s) 560, 588, 996
Tru9I TTAA 3 cut(s) 560, 588, 996
TscAI CASTG 3 cut(s) 42, 226, 917
TseFI GTSAC 2 cut(s) 33, 901
TseI GCWGC 1 cut(s) 708
Tsp45I GTSAC 2 cut(s) 33, 901
TspDTI ATGAA 4 cut(s) 26, 222, 297, 590
TspGWI ACGGA 2 cut(s) 627, 972
TspRI CASTG 3 cut(s) 42, 226, 917
Van91I CCANNNNNTGG 1 cut(s) 912
VpaK11BI GGWCC 4 cut(s) 195, 263, 355, 382
XapI RAATTY 1 cut(s) 814
XbaI TCTAGA 1 cut(s) 673
XceI RCATGY 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 2 cut(s) 674, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.