MD07G1264300.v1.1

BAG family molecular chaperone regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006122
Physical Location & Seq
Reverse (-)
33315224 .. 33317551
2328 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1264300.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGATGAGGATGAAGACGAAGACTACAGGGGTGTCACTGGCCGAGTTGAATGGAAGGTCAGGCGGCGGCGGAGCTGACTCGGGGCAGGGAGAGTGGGAGCTCAGACCGGGAGGAATGCTGGTTCAGAAACGGAATCCGGACTCTGATCGGAACTCGGTGCCTCCACCGACAATTCGGGTTCGGGTCAAATATGGGTCAATTTACCATGAAATCAGCATCAGCGCTCAATCTTCTTTTGGGGATTTGAAGAAAATGTTGGTTGGACCGACTGGTTTGCACCATCAAGATCAGAAATTGATATTCAAAGACAAGGAGAGGGACTCGAAGGCTTTTCTCGACATGTCTGGGGTGAAGGACCGGTCGAAGATGGTGTTAGTTGAGGACCCTATTAGCCAAGAAAAGCGATACCTTGAGATGCGACGCAATGCCAAGATGGAAAAGGCTTCGAAGTCCATCTCCGAAATCAGCTTGGAAGTAGACAGGCTTGCTGGCCAGGTGTCGGCTCTTGAATCAATTATCAGTAAAGGTAAAAAAGTTGCAGAGCAAGATGTGCTTGTTCTGATTGATCAGTTGATGAACCAGTTGCTTAAATTAGACGGAATCATGGGAGACGGTGATGTGAAACTACAGAGGAAAATGCAGGTAAAACGCGTGCAGAAGTATGTCGAAACTCTAGATGTATTGAAAGTTAAAAACTCCTTGCCTAGCAGCAACGGAAGTCAGATTCCAAAGCAAGTTCAGCAGAGGCATTCAAATGGTCATTCCAATGGGAATGGACATAGACTAGCACCGATTCAAGAGCATCAACCAAGGCGTTCAGTTGGCTATTCACCTACGCATAATCAGCAACTGCAACAGCAAGAACAACCGTCAGGGCAGTCGGCATCAGGGCCGGTAGTTGTGACCACACAGTGGGAGACATTCGATTCTGCCCCGGCATTGATCCCAGCCTCTTCGACATCCAAGGCCGCCAATCAATCTCCCTACCCTAGGTTTAATTGGGAATCGTTTGAGTAA

Protein Analysis

339

Amino Acids

37.5

Weight (kDa)

9.62

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAG PF02179 152 - 227 1.8e-16 BAG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 631
AccB1I GGYRCC 1 cut(s) 157
AccB7I CCANNNNNTGG 1 cut(s) 912
AccI GTMKAC 1 cut(s) 477
AccII CGCG 1 cut(s) 651
AccIII TCCGGA 1 cut(s) 136
AciI CCGC 4 cut(s) 63, 66, 69, 969
AclWI GGATC 1 cut(s) 937
AcoI YGGCCR 2 cut(s) 39, 490
AdeI CACNNNGTG 1 cut(s) 912
AfeI AGCGCT 1 cut(s) 223
AfiI CCNNNNNNNGG 3 cut(s) 499, 912, 990
AflIII ACRYGT 2 cut(s) 339, 649
AgeI ACCGGT 1 cut(s) 357
AgsI TTSAA 7 cut(s) 49, 247, 304, 509, 685, 753, 797
AjnI CCWGG 1 cut(s) 492
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
AloI GAACNNNNNNTCC 2 cut(s) 105, 137
AluBI AGCT 3 cut(s) 74, 100, 468
AluI AGCT 3 cut(s) 74, 100, 468
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 2 cut(s) 603, 911
AlwI GGATC 1 cut(s) 937
AlwNI CAGNNNCTG 1 cut(s) 850
Ama87I CYCGRG 1 cut(s) 79
Aor13HI TCCGGA 1 cut(s) 136
Aor51HI AGCGCT 1 cut(s) 223
AoxI GGCC 4 cut(s) 39, 490, 890, 966
ApeKI GCWGC 1 cut(s) 708
AsiGI ACCGGT 1 cut(s) 357
AspA2I CCTAGG 1 cut(s) 989
AspLEI GCGC 1 cut(s) 224
AspS9I GGNCC 4 cut(s) 263, 355, 382, 890
AsuC2I CCSGG 2 cut(s) 108, 935
AsuHPI GGTGA 3 cut(s) 361, 626, 822
AsuII TTCGAA 1 cut(s) 446
AvaI CYCGRG 1 cut(s) 79
AvaII GGWCC 3 cut(s) 263, 355, 382
AvrII CCTAGG 1 cut(s) 989
BalI TGGCCA 1 cut(s) 492
BanI GGYRCC 1 cut(s) 157
BanII GRGCYC 1 cut(s) 102
BbsI GAAGAC 2 cut(s) 20, 26
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 720
BccI CCATC 4 cut(s) 288, 361, 427, 461
BcgI CGANNNNNNTGC 2 cut(s) 256, 290
BciT130I CCWGG 1 cut(s) 494
BclI TGATCA 1 cut(s) 565
BcnI CCSGG 2 cut(s) 108, 935
BcoDI GTCTC 2 cut(s) 603, 911
BfaI CTAG 4 cut(s) 674, 705, 785, 990
BfmI CTRYAG 2 cut(s) 24, 626
BfoI RGCGCY 1 cut(s) 225
BfuAI ACCTGC 1 cut(s) 631
BisI GCNGC 4 cut(s) 64, 67, 709, 969
BlnI CCTAGG 1 cut(s) 989
BlsI GCNGC 4 cut(s) 65, 68, 710, 970
Bme1390I CCNGG 3 cut(s) 108, 494, 935
Bme18I GGWCC 3 cut(s) 263, 355, 382
BmeT110I CYCGRG 1 cut(s) 79
BmgT120I GGNCC 4 cut(s) 263, 355, 382, 890
BmiI GGNNCC 2 cut(s) 159, 384
BmrFI CCNGG 3 cut(s) 108, 494, 935
BmsI GCATC 4 cut(s) 225, 405, 811, 893
BpiI GAAGAC 2 cut(s) 20, 26
BplI GAGNNNNNCTC 2 cut(s) 305, 337
Bpu14I TTCGAA 1 cut(s) 446
BpuEI CTTGAG 1 cut(s) 431
BpuMI CCSGG 2 cut(s) 108, 935
BsaJI CCNNGG 4 cut(s) 809, 933, 963, 989
BsaWI WCCGGW 2 cut(s) 136, 357
Bsc4I CCNNNNNNNGG 3 cut(s) 499, 912, 990
Bse118I RCCGGY 2 cut(s) 357, 892
Bse1I ACTGG 3 cut(s) 42, 274, 580
Bse3DI GCAATG 1 cut(s) 430
BseAI TCCGGA 1 cut(s) 136
BseBI CCWGG 1 cut(s) 494
BseDI CCNNGG 4 cut(s) 809, 933, 963, 989
BseGI GGATG 2 cut(s) 15, 959
BseLI CCNNNNNNNGG 3 cut(s) 499, 912, 990
BseMI GCAATG 1 cut(s) 430
BseMII CTCAG 1 cut(s) 115
BseNI ACTGG 3 cut(s) 42, 274, 580
BseXI GCAGC 1 cut(s) 720
BseYI CCCAGC 1 cut(s) 946
BsgI GTGCAG 1 cut(s) 674
Bsh1236I CGCG 1 cut(s) 651
Bsh1285I CGRYCG 1 cut(s) 362
BshFI GGCC 4 cut(s) 41, 492, 892, 968
BshNI GGYRCC 1 cut(s) 157
BshTI ACCGGT 1 cut(s) 357
BsiEI CGRYCG 1 cut(s) 362
BsiHKAI GWGCWC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 5 cut(s) 107, 137, 358, 893, 935
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 3 cut(s) 499, 912, 990
BsmAI GTCTC 2 cut(s) 603, 911
BsmBI CGTCTC 1 cut(s) 603
BsmFI GGGAC 1 cut(s) 332
BsmI GAATGC 2 cut(s) 120, 748
BsnI GGCC 4 cut(s) 41, 492, 892, 968
BsoBI CYCGRG 1 cut(s) 79
Bsp119I TTCGAA 1 cut(s) 446
Bsp1286I GDGCHC 1 cut(s) 102
Bsp13I TCCGGA 1 cut(s) 136
Bsp143I GATC 4 cut(s) 145, 286, 565, 942
BspACI CCGC 4 cut(s) 63, 66, 69, 969
BspANI GGCC 4 cut(s) 41, 492, 892, 968
BspCNI CTCAG 1 cut(s) 114
BspEI TCCGGA 1 cut(s) 136
BspFNI CGCG 1 cut(s) 651
BspLI GGNNCC 2 cut(s) 159, 384
BspMI ACCTGC 1 cut(s) 631
BspPI GGATC 1 cut(s) 937
BspT104I TTCGAA 1 cut(s) 446
BspT107I GGYRCC 1 cut(s) 157
BsrDI GCAATG 1 cut(s) 430
BsrFI RCCGGY 2 cut(s) 357, 892
BsrI ACTGG 3 cut(s) 42, 274, 580
BssAI RCCGGY 2 cut(s) 357, 892
BssECI CCNNGG 4 cut(s) 809, 933, 963, 989
BssMI GATC 4 cut(s) 145, 286, 565, 942
BssT1I CCWWGG 3 cut(s) 809, 963, 989
Bst2UI CCWGG 1 cut(s) 494
Bst4CI ACNGT 3 cut(s) 614, 870, 912
Bst6I CTCTTC 1 cut(s) 958
BstAPI GCANNNNNTGC 1 cut(s) 550
BstBI TTCGAA 1 cut(s) 446
BstC8I GCNNGC 3 cut(s) 486, 490, 653
BstDEI CTNAG 1 cut(s) 101
BstENI CCTNNNNNAGG 1 cut(s) 988
BstF5I GGATG 2 cut(s) 15, 959
BstFNI CGCG 1 cut(s) 651
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 4 cut(s) 148, 289, 568, 945
BstMAI GTCTC 2 cut(s) 603, 911
BstMBI GATC 4 cut(s) 145, 286, 565, 942
BstMCI CGRYCG 1 cut(s) 362
BstMWI GCNNNNNNNGC 3 cut(s) 550, 739, 844
BstNI CCWGG 1 cut(s) 494
BstNSI RCATGY 1 cut(s) 343
BstSCI CCNGG 3 cut(s) 106, 492, 933
BstSFI CTRYAG 2 cut(s) 24, 626
BstUI CGCG 1 cut(s) 651
BstV1I GCAGC 1 cut(s) 720
BstV2I GAAGAC 2 cut(s) 20, 26
BsuRI GGCC 4 cut(s) 41, 492, 892, 968
BtsCI GGATG 2 cut(s) 15, 959
BtsIMutI CAGTG 2 cut(s) 35, 917
BveI ACCTGC 1 cut(s) 631
Cac8I GCNNGC 3 cut(s) 486, 490, 653
CaiI CAGNNNCTG 1 cut(s) 850
CfoI GCGC 1 cut(s) 224
Cfr10I RCCGGY 2 cut(s) 357, 892
Cfr13I GGNCC 4 cut(s) 263, 355, 382, 890
CseI GACGC 1 cut(s) 429
CspAI ACCGGT 1 cut(s) 357
CviAII CATG 3 cut(s) 206, 340, 604
DdeI CTNAG 1 cut(s) 101
DpnI GATC 4 cut(s) 147, 288, 567, 944
DpnII GATC 4 cut(s) 145, 286, 565, 942
DraIII CACNNNGTG 1 cut(s) 912
EaeI YGGCCR 2 cut(s) 39, 490
Eam1104I CTCTTC 1 cut(s) 958
EarI CTCTTC 1 cut(s) 958
EciI GGCGGA 1 cut(s) 84
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 3 cut(s) 809, 963, 989
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 3 cut(s) 263, 355, 382
Eco47III AGCGCT 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 100
Eco88I CYCGRG 1 cut(s) 79
EcoICRI GAGCTC 1 cut(s) 100
EcoNI CCTNNNNNAGG 1 cut(s) 988
EcoO109I RGGNCCY 1 cut(s) 382
EcoRII CCWGG 1 cut(s) 492
EcoT14I CCWWGG 3 cut(s) 809, 963, 989
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 3 cut(s) 809, 963, 989
Esp3I CGTCTC 1 cut(s) 603
FaeI CATG 3 cut(s) 209, 343, 607
FaiI YATR 7 cut(s) 192, 207, 341, 605, 663, 780, 840
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FaqI GGGAC 1 cut(s) 332
FatI CATG 3 cut(s) 205, 339, 603
FbaI TGATCA 1 cut(s) 565
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 4 cut(s) 64, 67, 709, 969
FokI GGATG 2 cut(s) 22, 946
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 4 cut(s) 64, 67, 709, 969
FspBI CTAG 4 cut(s) 674, 705, 785, 990
GlaI GCGC 1 cut(s) 223
GluI GCNGC 4 cut(s) 64, 67, 709, 969
GsaI CCCAGC 1 cut(s) 950
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 4 cut(s) 41, 492, 892, 968
HapII CCGG 5 cut(s) 107, 137, 358, 893, 935
HgaI GACGC 1 cut(s) 429
HhaI GCGC 1 cut(s) 224
Hin1II CATG 3 cut(s) 209, 343, 607
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HpaII CCGG 5 cut(s) 107, 137, 358, 893, 935
HphI GGTGA 3 cut(s) 361, 626, 822
Hpy166II GTNNAC 1 cut(s) 478
Hpy188I TCNGA 8 cut(s) 104, 126, 145, 150, 291, 460, 561, 723
Hpy188III TCNNGA 6 cut(s) 137, 284, 335, 506, 674, 797
Hpy8I GTNNAC 1 cut(s) 478
Hpy99I CGWCG 1 cut(s) 423
HpyAV CCTTC 3 cut(s) 48, 319, 346
HpyCH4III ACNGT 3 cut(s) 614, 870, 912
HpyCH4V TGCA 5 cut(s) 277, 539, 640, 655, 853
HpyF10VI GCNNNNNNNGC 3 cut(s) 550, 739, 844
HpyF3I CTNAG 1 cut(s) 101
Hsp92II CATG 3 cut(s) 209, 343, 607
HspAI GCGC 1 cut(s) 222
Kpn2I TCCGGA 1 cut(s) 136
Ksp22I TGATCA 1 cut(s) 565
Kzo9I GATC 4 cut(s) 145, 286, 565, 942
LmnI GCTCC 2 cut(s) 71, 97
Lsp1109I GCAGC 1 cut(s) 720
LweI GCATC 4 cut(s) 225, 405, 811, 893
MaeI CTAG 4 cut(s) 674, 705, 785, 990
MaeIII GTNAC 2 cut(s) 33, 901
MalI GATC 4 cut(s) 147, 288, 567, 944
MboI GATC 4 cut(s) 145, 286, 565, 942
MboII GAAGA 6 cut(s) 25, 31, 222, 259, 376, 945
MhlI GDGCHC 1 cut(s) 102
MlsI TGGCCA 1 cut(s) 492
MluCI AATT 6 cut(s) 171, 198, 293, 513, 590, 997
MluI ACGCGT 1 cut(s) 649
MluNI TGGCCA 1 cut(s) 492
MlyI GAGTC 3 cut(s) 71, 134, 314
MmeI TCCRAC 1 cut(s) 241
MnlI CCTC 7 cut(s) 104, 171, 309, 373, 624, 738, 961
Mox20I TGGCCA 1 cut(s) 492
MroI TCCGGA 1 cut(s) 136
MscI TGGCCA 1 cut(s) 492
MseI TTAA 3 cut(s) 588, 690, 996
MslI CAYNNNNRTG 2 cut(s) 753, 765
Msp20I TGGCCA 1 cut(s) 492
MspI CCGG 5 cut(s) 107, 137, 358, 893, 935
MspR9I CCNGG 3 cut(s) 108, 494, 935
Mva1269I GAATGC 2 cut(s) 120, 748
MvaI CCWGG 1 cut(s) 494
MvnI CGCG 1 cut(s) 651
MwoI GCNNNNNNNGC 3 cut(s) 550, 739, 844
NciI CCSGG 2 cut(s) 108, 935
NdeII GATC 4 cut(s) 145, 286, 565, 942
NlaIII CATG 3 cut(s) 209, 343, 607
NlaIV GGNNCC 2 cut(s) 159, 384
NmeAIII GCCGAG 1 cut(s) 67
NmuCI GTSAC 2 cut(s) 33, 901
NspI RCATGY 1 cut(s) 343
NspV TTCGAA 1 cut(s) 446
PciI ACATGT 1 cut(s) 339
PctI GAATGC 2 cut(s) 120, 748
PfeI GAWTC 7 cut(s) 133, 509, 600, 724, 793, 926, 1004
PflMI CCANNNNNTGG 1 cut(s) 912
PinAI ACCGGT 1 cut(s) 357
PkrI GCNGC 4 cut(s) 65, 68, 710, 970
PleI GAGTC 3 cut(s) 71, 134, 314
PpsI GAGTC 3 cut(s) 71, 134, 314
PpuMI RGGWCCY 1 cut(s) 382
PscI ACATGT 1 cut(s) 339
Psp124BI GAGCTC 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 492
PspFI CCCAGC 1 cut(s) 946
PspGI CCWGG 1 cut(s) 492
PspN4I GGNNCC 2 cut(s) 159, 384
PspPI GGNCC 4 cut(s) 263, 355, 382, 890
PspPPI RGGWCCY 1 cut(s) 382
PstNI CAGNNNCTG 1 cut(s) 850
RseI CAYNNNNRTG 2 cut(s) 753, 765
SacI GAGCTC 1 cut(s) 102
SaqAI TTAA 3 cut(s) 588, 690, 996
SatI GCNGC 4 cut(s) 64, 67, 709, 969
Sau3AI GATC 4 cut(s) 145, 286, 565, 942
Sau96I GGNCC 4 cut(s) 263, 355, 382, 890
SchI GAGTC 3 cut(s) 71, 134, 314
ScrFI CCNGG 3 cut(s) 108, 494, 935
SduI GDGCHC 1 cut(s) 102
SfaNI GCATC 4 cut(s) 225, 405, 811, 893
SfcI CTRYAG 2 cut(s) 24, 626
SfuI TTCGAA 1 cut(s) 446
SinI GGWCC 3 cut(s) 263, 355, 382
SmiMI CAYNNNNRTG 2 cut(s) 753, 765
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 6 cut(s) 171, 198, 293, 513, 590, 997
SsiI CCGC 4 cut(s) 63, 66, 69, 969
SspMI CTAG 4 cut(s) 674, 705, 785, 990
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 3 cut(s) 106, 492, 933
StyI CCWWGG 3 cut(s) 809, 963, 989
TaaI ACNGT 3 cut(s) 614, 870, 912
TaqI TCGA 7 cut(s) 323, 336, 362, 446, 666, 924, 956
TaqII GACCGA 1 cut(s) 280
TasI AATT 6 cut(s) 171, 198, 293, 513, 590, 997
TauI GCSGC 3 cut(s) 66, 69, 971
TfiI GAWTC 7 cut(s) 133, 509, 600, 724, 793, 926, 1004
Tru1I TTAA 3 cut(s) 588, 690, 996
Tru9I TTAA 3 cut(s) 588, 690, 996
TscAI CASTG 2 cut(s) 42, 917
TseFI GTSAC 2 cut(s) 33, 901
TseI GCWGC 1 cut(s) 708
Tsp45I GTSAC 2 cut(s) 33, 901
TspDTI ATGAA 3 cut(s) 26, 222, 590
TspGWI ACGGA 3 cut(s) 145, 612, 729
TspRI CASTG 2 cut(s) 42, 917
Van91I CCANNNNNTGG 1 cut(s) 912
VpaK11BI GGWCC 3 cut(s) 263, 355, 382
XagI CCTNNNNNAGG 1 cut(s) 988
XbaI TCTAGA 1 cut(s) 673
XceI RCATGY 1 cut(s) 343
XmaJI CCTAGG 1 cut(s) 989
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 4 cut(s) 674, 705, 785, 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.