Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0012867

Physical Location & Seq

Genomic Coordinates Forward (+)

30413680 .. 30415409

Gene Structure 1730 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1210200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1413 bp

ATGGCAGATGATTGGGATCTTTACGCCGTTGTAAGGGGTTGCAAATCAACCGCCCAAACCACTACAAATACCACCGCCGCCGCCACCACCGCGATCGTACCCACAACCCCTATCCATACACCGTCGGATGAATTCGCAGACGACCCTTTTTGGGATTTGTTGATCAGTTCCGACGAGTTAACCGATCCGTTGTTCTCTTTTCCCGATCTAGACGATCCCAAAGACAATGATTTGCAAGAGCAGCAACAGTCCTTTTTCCCCAACCCCACCACCACCAACAACAACAAGAACAACAATCCTAGCACTAACAACACCACCTTTGGACCTAATAATATTATTCCAATTTCCCCCACTATTTCTGACTTTGGAGGATCTAGTGGTAGTGGCCAACATCACCTCCAACAACATCAACAGCAACATCATGCACAACTCCAACAACAGCTGCAGCAGCAGCAGAACCAGCAACATCATTTGATGCCTGATCATTTCACCCGGGGAATACCGGCTCCTGCTTTTCGAGGAATCTATGGCCAACAACAACAGCAAATGATGCAGCGGGGGTATCAAATTCTTCAACCCCGGCAGCCCCAAAATCAGCAGGGATTTATGAGGCCCGGGTCCATGAATCCCGCTTATCCTCTTCCTCGGCTACCAATAACCATGCCATTGCACACCCCTCGCTCTCAAAGAAGAAGGAGAACCCAGCAAAATAAAAAGGTGTGTGAAGTGAAAGCTGAGGATCTCACGGCCGATTTATGGGCGTGGCGGAAGTACGGCCAAAAACCTATCAAGGGTTCTCCACATCCAAGGAACTATTACCGATGCAGCACCTCAAAAGGGTGTGCGGCGAGGAAGCATGTGGAGCGGAGCACGACGGATCCCAACATCTTCACAGTCACATACTCCGGCGACCACACTCACGCTCGCCCGACTCACCGGAACTCGCTTGCCGGTACCTCCAGGAACAAGCTGTCAGCTGCGTCTAAGTGTCAAAAGCAGCCAATTAACAACGATTCTGGTTCAGCAGCTGTTCCCAACCCCTCGTCTCCCACCACCGGACAACCCTTGACAGTCCAAGCTGAGGCTGTAGCCAATAAGGACAATTCTGACAATTCACTTGACAAAGAAAATGACGAATTTAATGATGACGATGAGGAGATGGATGAGACCGAGTACGGGGATGAGGACGAGGATGATGATGTTGTGATCCCGGACATGGACATGTCGGATGAGATATTCTTGGGGCAGAAGGAACTGGGTAGTACTAGTTCTAGCGGAAGTGGAAGTTCTTCTATGGTGGGTGCTTCCGGTGATAATTTTTTGGATAATGTGCAATCAAGTTTGGGGTCTTCTTGGACTGATGCTAATTGCTCCTCCGCTGCCGGAGCAACCGTTGGCAGTGGTTGCTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007263 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009739 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0019932 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0033993 GO:0035556 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

471

Amino Acids

51.76

Weight (kDa)

5.08

Isoelectric Point (pI)

48.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	251 - 307	2.8e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012867)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52830
fragaria_vesca	FvH4_7g30460
malus_domestica	MD01G1210200.v1.1 MD07G1280300.v1.1
prunus_persica	Prupe.2G302500_v2.0.a1
pyrus_communis	pycom01g22070 pycom07g25490
rosa_chinensis	RchiOBHm_Chr1g0378621
rosa_laevigata	RLG00000026418
rosa_multiflora	Rmu_sc0002395.1_g000012
rosa_roxburghii	Rroxscaffold_4G00280350
rosa_rugosa	Rorug01G0410000 Rorug01G0410100
rosa_samantha	Rh1AG431500 Rh1BG389800 Rh1CG403100 Rh1DG421000
rosa_wichuraiana	Rw1G037800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	953
AccB1I	GGYRCC	1 cut(s)	953
AccBSI	CCGCTC	1 cut(s)	865
AccII	CGCG	1 cut(s)	92
AclWI	GGATC	8 cut(s)	24, 179, 209, 379, 747, 872, 885, 1201
AcoI	YGGCCR	4 cut(s)	385, 529, 747, 775
AcsI	RAATTY	3 cut(s)	131, 567, 1136
AfaI	GTAC	5 cut(s)	99, 773, 955, 1175, 1264
AfiI	CCNNNNNNNGG	9 cut(s)	33, 151, 756, 791, 837, 1055, 1081, 1176, 1216
AflIII	ACRYGT	1 cut(s)	1221
AgsI	TTSAA	1 cut(s)	575
AhlI	ACTAGT	1 cut(s)	1265
AjnI	CCWGG	1 cut(s)	959
AluBI	AGCT	6 cut(s)	442, 734, 970, 977, 1028, 1079
AluI	AGCT	6 cut(s)	442, 734, 970, 977, 1028, 1079
Alw21I	GWGCWC	1 cut(s)	872
Alw26I	GTCTC	2 cut(s)	1050, 1160
AlwI	GGATC	8 cut(s)	24, 179, 209, 379, 747, 872, 885, 1201
AlwNI	CAGNNNCTG	1 cut(s)	1028
Ama87I	CYCGRG	2 cut(s)	492, 614
AoxI	GGCC	5 cut(s)	385, 529, 611, 747, 775
ApoI	RAATTY	3 cut(s)	131, 567, 1136
Asp700I	GAANNNNTTC	1 cut(s)	1288
Asp718I	GGTACC	1 cut(s)	953
AspS9I	GGNCC	3 cut(s)	323, 612, 618
AsuC2I	CCSGG	6 cut(s)	493, 494, 580, 615, 616, 1211
AsuHPI	GGTGA	4 cut(s)	386, 481, 926, 1322
AvaI	CYCGRG	2 cut(s)	492, 614
AvaII	GGWCC	2 cut(s)	323, 618
BalI	TGGCCA	2 cut(s)	387, 531
BamHI	GGATCC	1 cut(s)	877
BanI	GGYRCC	1 cut(s)	953
BbsI	GAAGAC	1 cut(s)	1341
Bbv12I	GWGCWC	1 cut(s)	872
BbvCI	CCTCAGC	2 cut(s)	735, 1080
BccI	CCATC	1 cut(s)	1153
BceAI	ACGGC	3 cut(s)	11, 762, 790
BcgI	CGANNNNNNTGC	2 cut(s)	659, 693
BciT130I	CCWGG	1 cut(s)	961
BclI	TGATCA	2 cut(s)	162, 481
BcnI	CCSGG	6 cut(s)	493, 494, 580, 615, 616, 1211
BcoDI	GTCTC	2 cut(s)	1050, 1160
BcuI	ACTAGT	1 cut(s)	1265
BfaI	CTAG	5 cut(s)	209, 300, 375, 1266, 1272
BfmI	CTRYAG	2 cut(s)	443, 1086
BmcAI	AGTACT	1 cut(s)	1264
Bme1390I	CCNGG	7 cut(s)	493, 494, 580, 615, 616, 961, 1211
Bme18I	GGWCC	2 cut(s)	323, 618
BmeT110I	CYCGRG	2 cut(s)	492, 614
BmgT120I	GGNCC	3 cut(s)	323, 612, 618
BmiI	GGNNCC	4 cut(s)	507, 619, 879, 955
BmrFI	CCNGG	7 cut(s)	493, 494, 580, 615, 616, 961, 1211
BmrI	ACTGGG	1 cut(s)	1265
BmsI	GCATC	4 cut(s)	465, 540, 812, 1351
BmuI	ACTGGG	1 cut(s)	1265
BpiI	GAAGAC	1 cut(s)	1341
BpmI	CTGGAG	1 cut(s)	943
Bpu10I	CCTNAGC	2 cut(s)	735, 1080
BpuMI	CCSGG	6 cut(s)	493, 494, 580, 615, 616, 1211
BsaBI	GATNNNNATC	1 cut(s)	15
BsaI	GGTCTC	1 cut(s)	1160
BsaJI	CCNNGG	6 cut(s)	492, 493, 578, 614, 644, 806
BsaWI	WCCGGW	3 cut(s)	936, 1055, 1307
BsaXI	ACNNNNNCTCC	4 cut(s)	381, 411, 1377, 1407
Bsc4I	CCNNNNNNNGG	9 cut(s)	33, 151, 756, 791, 837, 1055, 1081, 1176, 1216
Bse118I	RCCGGY	2 cut(s)	502, 950
Bse1I	ACTGG	1 cut(s)	1260
Bse3DI	GCAATG	1 cut(s)	665
Bse8I	GATNNNNATC	1 cut(s)	15
BseBI	CCWGG	1 cut(s)	961
BseDI	CCNNGG	6 cut(s)	492, 493, 578, 614, 644, 806
BseGI	GGATG	6 cut(s)	133, 802, 1168, 1186, 1198, 1234
BseJI	GATNNNNATC	1 cut(s)	15
BseLI	CCNNNNNNNGG	9 cut(s)	33, 151, 756, 791, 837, 1055, 1081, 1176, 1216
BseMI	GCAATG	1 cut(s)	665
BseMII	CTCAG	2 cut(s)	726, 1071
BseNI	ACTGG	1 cut(s)	1260
BseRI	GAGGAG	2 cut(s)	1169, 1363
BseX3I	CGGCCG	1 cut(s)	747
BseYI	CCCAGC	1 cut(s)	702
Bsh1236I	CGCG	1 cut(s)	92
Bsh1285I	CGRYCG	2 cut(s)	96, 750
BshFI	GGCC	5 cut(s)	387, 531, 613, 749, 777
BshNI	GGYRCC	1 cut(s)	953
BsiEI	CGRYCG	2 cut(s)	96, 750
BsiHKAI	GWGCWC	1 cut(s)	872
BsiHKCI	CYCGRG	2 cut(s)	492, 614
BslI	CCNNNNNNNGG	9 cut(s)	33, 151, 756, 791, 837, 1055, 1081, 1176, 1216
BsmAI	GTCTC	2 cut(s)	1050, 1160
BsmBI	CGTCTC	1 cut(s)	1050
BsnI	GGCC	5 cut(s)	387, 531, 613, 749, 777
Bso31I	GGTCTC	1 cut(s)	1160
BsoBI	CYCGRG	2 cut(s)	492, 614
Bsp1286I	GDGCHC	1 cut(s)	872
BspANI	GGCC	5 cut(s)	387, 531, 613, 749, 777
BspCNI	CTCAG	2 cut(s)	727, 1072
BspFNI	CGCG	1 cut(s)	92
BspLI	GGNNCC	4 cut(s)	507, 619, 879, 955
BspMAI	CTGCAG	1 cut(s)	447
BspPI	GGATC	8 cut(s)	24, 179, 209, 379, 747, 872, 885, 1201
BspT107I	GGYRCC	1 cut(s)	953
BspTNI	GGTCTC	1 cut(s)	1160
BsrBI	CCGCTC	1 cut(s)	865
BsrDI	GCAATG	1 cut(s)	665
BsrFI	RCCGGY	2 cut(s)	502, 950
BsrI	ACTGG	1 cut(s)	1260
BssAI	RCCGGY	2 cut(s)	502, 950
BssECI	CCNNGG	6 cut(s)	492, 493, 578, 614, 644, 806
BssT1I	CCWWGG	1 cut(s)	806
Bst2UI	CCWGG	1 cut(s)	961
Bst4CI	ACNGT	5 cut(s)	123, 249, 895, 1072, 1393
Bst6I	CTCTTC	1 cut(s)	645
BstAPI	GCANNNNNTGC	2 cut(s)	550, 1404
BstC8I	GCNNGC	2 cut(s)	925, 948
BstDEI	CTNAG	3 cut(s)	735, 984, 1080
BstF5I	GGATG	6 cut(s)	133, 802, 1168, 1186, 1198, 1234
BstFNI	CGCG	1 cut(s)	92
BstMAI	GTCTC	2 cut(s)	1050, 1160
BstMCI	CGRYCG	2 cut(s)	96, 750
BstMWI	GCNNNNNNNGC	9 cut(s)	89, 241, 448, 451, 460, 550, 862, 1385, 1404
BstNI	CCWGG	1 cut(s)	961
BstNSI	RCATGY	2 cut(s)	860, 1225
BstSCI	CCNGG	7 cut(s)	491, 492, 578, 613, 614, 959, 1209
BstSFI	CTRYAG	2 cut(s)	443, 1086
BstUI	CGCG	1 cut(s)	92
BstV2I	GAAGAC	1 cut(s)	1341
BstX2I	RGATCY	4 cut(s)	16, 371, 739, 877
BstYI	RGATCY	4 cut(s)	16, 371, 739, 877
BstZI	CGGCCG	1 cut(s)	747
BsuRI	GGCC	5 cut(s)	387, 531, 613, 749, 777
BtsCI	GGATG	6 cut(s)	133, 802, 1168, 1186, 1198, 1234
BtsI	GCAGTG	1 cut(s)	1405
BtsIMutI	CAGTG	1 cut(s)	1405
Cac8I	GCNNGC	2 cut(s)	925, 948
CaiI	CAGNNNCTG	1 cut(s)	1028
Cfr10I	RCCGGY	2 cut(s)	502, 950
Cfr13I	GGNCC	3 cut(s)	323, 612, 618
Cfr9I	CCCGGG	2 cut(s)	492, 614
CseI	GACGC	1 cut(s)	969
Csp6I	GTAC	5 cut(s)	98, 772, 954, 1174, 1263
CviAII	CATG	6 cut(s)	422, 622, 661, 857, 1216, 1222
CviQI	GTAC	5 cut(s)	98, 772, 954, 1174, 1263
DdeI	CTNAG	3 cut(s)	735, 984, 1080
EaeI	YGGCCR	4 cut(s)	385, 529, 747, 775
EagI	CGGCCG	1 cut(s)	747
Eam1104I	CTCTTC	1 cut(s)	645
EarI	CTCTTC	1 cut(s)	645
EciI	GGCGGA	1 cut(s)	781
EclXI	CGGCCG	1 cut(s)	747
Eco130I	CCWWGG	1 cut(s)	806
Eco31I	GGTCTC	1 cut(s)	1160
Eco47I	GGWCC	2 cut(s)	323, 618
Eco52I	CGGCCG	1 cut(s)	747
Eco88I	CYCGRG	2 cut(s)	492, 614
EcoRI	GAATTC	1 cut(s)	131
EcoRII	CCWGG	1 cut(s)	959
EcoT14I	CCWWGG	1 cut(s)	806
ErhI	CCWWGG	1 cut(s)	806
Esp3I	CGTCTC	1 cut(s)	1050
FaeI	CATG	6 cut(s)	425, 625, 664, 860, 1219, 1225
FatI	CATG	6 cut(s)	421, 621, 660, 856, 1215, 1221
FauI	CCCGC	2 cut(s)	549, 637
FbaI	TGATCA	2 cut(s)	162, 481
FokI	GGATG	6 cut(s)	140, 789, 1175, 1193, 1205, 1241
FspBI	CTAG	5 cut(s)	209, 300, 375, 1266, 1272
GsaI	CCCAGC	1 cut(s)	706
GsuI	CTGGAG	1 cut(s)	943
HaeIII	GGCC	5 cut(s)	387, 531, 613, 749, 777
HgaI	GACGC	1 cut(s)	969
Hin1II	CATG	6 cut(s)	425, 625, 664, 860, 1219, 1225
HincII	GTYRAC	1 cut(s)	180
HindII	GTYRAC	1 cut(s)	180
HinfI	GANTC	4 cut(s)	522, 625, 931, 1013
HpaI	GTTAAC	1 cut(s)	180
HphI	GGTGA	4 cut(s)	386, 481, 926, 1322
Hpy166II	GTNNAC	1 cut(s)	180
Hpy188I	TCNGA	5 cut(s)	127, 172, 361, 1108, 1228
Hpy188III	TCNNGA	2 cut(s)	203, 209
Hpy8I	GTNNAC	1 cut(s)	180
Hpy99I	CGWCG	3 cut(s)	127, 176, 877
HpyAV	CCTTC	2 cut(s)	687, 1243
HpyCH4III	ACNGT	5 cut(s)	123, 249, 895, 1072, 1393
HpyCH4V	TGCA	8 cut(s)	42, 235, 425, 445, 553, 670, 825, 1333
HpyF10VI	GCNNNNNNNGC	9 cut(s)	89, 241, 448, 451, 460, 550, 862, 1385, 1404
HpyF3I	CTNAG	3 cut(s)	735, 984, 1080
Hsp92II	CATG	6 cut(s)	425, 625, 664, 860, 1219, 1225
KpnI	GGTACC	1 cut(s)	957
Ksp22I	TGATCA	2 cut(s)	162, 481
KspAI	GTTAAC	1 cut(s)	180
LmnI	GCTCC	5 cut(s)	511, 862, 867, 1376, 1385
LweI	GCATC	4 cut(s)	465, 540, 812, 1351
MaeI	CTAG	5 cut(s)	209, 300, 375, 1266, 1272
MaeIII	GTNAC	1 cut(s)	895
MbiI	CCGCTC	1 cut(s)	865
MboII	GAAGA	6 cut(s)	563, 632, 702, 880, 1281, 1341
MflI	RGATCY	4 cut(s)	16, 371, 739, 877
MhlI	GDGCHC	1 cut(s)	872
MlsI	TGGCCA	2 cut(s)	387, 531
MluCI	AATT	9 cut(s)	131, 342, 567, 1002, 1102, 1111, 1136, 1315, 1366
MluNI	TGGCCA	2 cut(s)	387, 531
MlyI	GAGTC	1 cut(s)	925
MmeI	TCCRAC	5 cut(s)	105, 195, 424, 457, 1206
Mox20I	TGGCCA	2 cut(s)	387, 531
MroXI	GAANNNNTTC	1 cut(s)	1288
MscI	TGGCCA	2 cut(s)	387, 531
MseI	TTAA	3 cut(s)	179, 1005, 1140
MslI	CAYNNNNRTG	1 cut(s)	1220
Msp20I	TGGCCA	2 cut(s)	387, 531
MspA1I	CMGCKG	5 cut(s)	442, 556, 977, 1028, 1379
MspR9I	CCNGG	7 cut(s)	493, 494, 580, 615, 616, 961, 1211
MvaI	CCWGG	1 cut(s)	961
MvnI	CGCG	1 cut(s)	92
MwoI	GCNNNNNNNGC	9 cut(s)	89, 241, 448, 451, 460, 550, 862, 1385, 1404
NciI	CCSGG	6 cut(s)	493, 494, 580, 615, 616, 1211
NlaIII	CATG	6 cut(s)	425, 625, 664, 860, 1219, 1225
NlaIV	GGNNCC	4 cut(s)	507, 619, 879, 955
NmeAIII	GCCGAG	1 cut(s)	625
NmuCI	GTSAC	1 cut(s)	895
NspI	RCATGY	2 cut(s)	860, 1225
PciI	ACATGT	1 cut(s)	1221
PcsI	WCGNNNNNNNCGW	1 cut(s)	180
PdmI	GAANNNNTTC	1 cut(s)	1288
PfeI	GAWTC	3 cut(s)	522, 625, 1013
PfoI	TCCNGGA	2 cut(s)	959, 1209
Ple19I	CGATCG	1 cut(s)	96
PleI	GAGTC	1 cut(s)	925
PpsI	GAGTC	1 cut(s)	925
PscI	ACATGT	1 cut(s)	1221
Psp6I	CCWGG	1 cut(s)	959
PspFI	CCCAGC	1 cut(s)	702
PspGI	CCWGG	1 cut(s)	959
PspN4I	GGNNCC	4 cut(s)	507, 619, 879, 955
PspPI	GGNCC	3 cut(s)	323, 612, 618
PsrI	GAACNNNNNNTAC	2 cut(s)	1252, 1284
PstI	CTGCAG	1 cut(s)	447
PstNI	CAGNNNCTG	1 cut(s)	1028
PsuI	RGATCY	4 cut(s)	16, 371, 739, 877
PvuI	CGATCG	1 cut(s)	96
PvuII	CAGCTG	3 cut(s)	442, 977, 1028
RsaI	GTAC	5 cut(s)	99, 773, 955, 1175, 1264
RsaNI	GTAC	5 cut(s)	98, 772, 954, 1174, 1263
RseI	CAYNNNNRTG	1 cut(s)	1220
SaqAI	TTAA	3 cut(s)	179, 1005, 1140
Sau96I	GGNCC	3 cut(s)	323, 612, 618
ScaI	AGTACT	1 cut(s)	1264
SchI	GAGTC	1 cut(s)	925
ScrFI	CCNGG	7 cut(s)	493, 494, 580, 615, 616, 961, 1211
SduI	GDGCHC	1 cut(s)	872
SfaNI	GCATC	4 cut(s)	465, 540, 812, 1351
SfcI	CTRYAG	2 cut(s)	443, 1086
SinI	GGWCC	2 cut(s)	323, 618
SmaI	CCCGGG	2 cut(s)	494, 616
SmiMI	CAYNNNNRTG	1 cut(s)	1220
SpeI	ACTAGT	1 cut(s)	1265
Sse9I	AATT	9 cut(s)	131, 342, 567, 1002, 1102, 1111, 1136, 1315, 1366
SspI	AATATT	1 cut(s)	334
SspMI	CTAG	5 cut(s)	209, 300, 375, 1266, 1272
StyD4I	CCNGG	7 cut(s)	491, 492, 578, 613, 614, 959, 1209
StyI	CCWWGG	1 cut(s)	806
TaaI	ACNGT	5 cut(s)	123, 249, 895, 1072, 1393
TaqI	TCGA	1 cut(s)	517
TaqII	GACCGA	1 cut(s)	1184
TasI	AATT	9 cut(s)	131, 342, 567, 1002, 1102, 1111, 1136, 1315, 1366
TatI	WGTACW	1 cut(s)	1262
TauI	GCSGC	3 cut(s)	80, 83, 848
TfiI	GAWTC	3 cut(s)	522, 625, 1013
Tru1I	TTAA	3 cut(s)	179, 1005, 1140
Tru9I	TTAA	3 cut(s)	179, 1005, 1140
TscAI	CASTG	1 cut(s)	1405
TseFI	GTSAC	1 cut(s)	895
Tsp45I	GTSAC	1 cut(s)	895
TspDTI	ATGAA	2 cut(s)	144, 638
TspGWI	ACGGA	2 cut(s)	177, 890
TspMI	CCCGGG	2 cut(s)	492, 614
TspRI	CASTG	1 cut(s)	1405
VpaK11BI	GGWCC	2 cut(s)	323, 618
XapI	RAATTY	3 cut(s)	131, 567, 1136
XbaI	TCTAGA	1 cut(s)	208
XceI	RCATGY	2 cut(s)	860, 1225
XmaI	CCCGGG	2 cut(s)	492, 614
XmnI	GAANNNNTTC	1 cut(s)	1288
XspI	CTAG	5 cut(s)	209, 300, 375, 1266, 1272
ZrmI	AGTACT	1 cut(s)	1264

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.