Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0012867

Physical Location & Seq

Genomic Coordinates Forward (+)

34424687 .. 34425782

Gene Structure 1096 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1280300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 837 bp

ATGCCTACTCATTTCACCCGGGGAATTCCAGCTCCTAGTTTTCGAGGAATCTATGGCCAACAACAACAACAACAAAAAATTATGCAACCCGGGGATCAAAATCTTCAACCCCGCCAGCTCCAAACTCAGCAGGGATTTAGGAGGCCCAAGTTCATCAATCCCGCTTATCCTCATCCTCGGCTACCAACTCGCCCACAAAGAAGAATGAAAACCCAGCAAAAGAAAAATGTTCATGAAGTGAAAGCGGAGGATCTCATCAGCGCTGATTTATGGGCGTGGCGGAAGTACGGCCAAAAACCTATCAAGGGTTCTCCACACCCAAGGAACTACTACAGATGCAGCAGCTCAAAAGGGTGCCCGGCGAGAAGGCAAGTGGAGCAGAGCACGACAGAACCCAACATCTTCATAGTTACATACACCGGGGACCACACTCACGCTCGCCCGACTCATCGAAACTCCCTTGCCGGGAGCTCCAGGAACAAGCTGTCAGCTGCATCTACGAGTCAAAAGAAGCCAATCAACAACGACTCCGGTTCGACAGCTGTTCCCAACCCCTCGTCTCCCACCACCGGACTACCCATGACAGTCCAAGCTGAGGCTGTGCCTGTAGCCAATAAGGTCAATTCTGACAATTCACTTGACAAAGAAACTGAGGAATCTGATGAGGAGGAGGAGACGGATCATGAGAATGAGAACGAGAACGAGGTCGAGGACGATGACGTTATGATCCCGGACATGGACATGTCGGATGAGATATTCTTGGGGCTGAAGGAACTGGGTGGTACTAGTTCTAATGGCAGTGGGAGTGGAAGTTTCTCTGGTTTCTGCTTCCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007263 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009739 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0019932 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0033993 GO:0035556 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

279

Amino Acids

31.07

Weight (kDa)

7.1

Isoelectric Point (pI)

55.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	89 - 145	4.3e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012867)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52830
fragaria_vesca	FvH4_7g30460
malus_domestica	MD01G1210200.v1.1 MD07G1280300.v1.1
prunus_persica	Prupe.2G302500_v2.0.a1
pyrus_communis	pycom01g22070 pycom07g25490
rosa_chinensis	RchiOBHm_Chr1g0378621
rosa_laevigata	RLG00000026418
rosa_multiflora	Rmu_sc0002395.1_g000012
rosa_roxburghii	Rroxscaffold_4G00280350
rosa_rugosa	Rorug01G0410000 Rorug01G0410100
rosa_samantha	Rh1AG431500 Rh1BG389800 Rh1CG403100 Rh1DG421000
rosa_wichuraiana	Rw1G037800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	354
AciI	CCGC	4 cut(s)	112, 162, 245, 280
AclWI	GGATC	4 cut(s)	102, 258, 687, 721
AcoI	YGGCCR	2 cut(s)	55, 289
AcsI	RAATTY	1 cut(s)	24
AcuI	CTGAAG	1 cut(s)	788
AfaI	GTAC	2 cut(s)	287, 784
AfeI	AGCGCT	1 cut(s)	262
AfiI	CCNNNNNNNGG	5 cut(s)	305, 465, 569, 595, 736
AflIII	ACRYGT	1 cut(s)	741
AgsI	TTSAA	1 cut(s)	107
AhlI	ACTAGT	1 cut(s)	785
AjnI	CCWGG	1 cut(s)	473
AluBI	AGCT	8 cut(s)	32, 118, 345, 471, 484, 491, 542, 593
AluI	AGCT	8 cut(s)	32, 118, 345, 471, 484, 491, 542, 593
Alw21I	GWGCWC	2 cut(s)	386, 473
Alw26I	GTCTC	2 cut(s)	564, 668
AlwI	GGATC	4 cut(s)	102, 258, 687, 721
Ama87I	CYCGRG	2 cut(s)	18, 89
Aor51HI	AGCGCT	1 cut(s)	262
AoxI	GGCC	3 cut(s)	55, 143, 289
ApeKI	GCWGC	3 cut(s)	339, 342, 491
ApoI	RAATTY	1 cut(s)	24
AspLEI	GCGC	1 cut(s)	263
AspS9I	GGNCC	2 cut(s)	144, 424
AsuC2I	CCSGG	8 cut(s)	19, 20, 90, 91, 359, 421, 466, 731
AsuHPI	GGTGA	1 cut(s)	7
AvaI	CYCGRG	2 cut(s)	18, 89
AvaII	GGWCC	1 cut(s)	424
BaeGI	GKGCMC	1 cut(s)	359
BalI	TGGCCA	1 cut(s)	57
BanI	GGYRCC	1 cut(s)	354
BanII	GRGCYC	1 cut(s)	473
Bbv12I	GWGCWC	2 cut(s)	386, 473
BbvCI	CCTCAGC	1 cut(s)	594
BbvI	GCAGC	3 cut(s)	351, 354, 478
BceAI	ACGGC	1 cut(s)	304
BciT130I	CCWGG	1 cut(s)	475
BcnI	CCSGG	8 cut(s)	19, 20, 90, 91, 359, 421, 466, 731
BcoDI	GTCTC	2 cut(s)	564, 668
BcuI	ACTAGT	1 cut(s)	785
BfaI	CTAG	2 cut(s)	36, 786
BfmI	CTRYAG	2 cut(s)	331, 606
BfoI	RGCGCY	1 cut(s)	264
BisI	GCNGC	3 cut(s)	340, 343, 492
BlsI	GCNGC	3 cut(s)	341, 344, 493
Bme1390I	CCNGG	9 cut(s)	19, 20, 90, 91, 359, 421, 466, 475, 731
Bme18I	GGWCC	1 cut(s)	424
BmeT110I	CYCGRG	2 cut(s)	18, 89
BmgT120I	GGNCC	2 cut(s)	144, 424
BmiI	GGNNCC	2 cut(s)	356, 425
BmrFI	CCNGG	9 cut(s)	19, 20, 90, 91, 359, 421, 466, 475, 731
BmrI	ACTGGG	1 cut(s)	785
BmsI	GCATC	2 cut(s)	326, 503
BmuI	ACTGGG	1 cut(s)	785
BpmI	CTGGAG	1 cut(s)	457
Bpu10I	CCTNAGC	1 cut(s)	594
BpuMI	CCSGG	8 cut(s)	19, 20, 90, 91, 359, 421, 466, 731
BsaBI	GATNNNNATC	1 cut(s)	99
BsaJI	CCNNGG	7 cut(s)	18, 19, 89, 90, 176, 320, 420
BsaWI	WCCGGW	3 cut(s)	530, 569, 831
BsaXI	ACNNNNNCTCC	4 cut(s)	512, 542, 796, 826
Bsc4I	CCNNNNNNNGG	5 cut(s)	305, 465, 569, 595, 736
Bse1I	ACTGG	1 cut(s)	780
Bse8I	GATNNNNATC	1 cut(s)	99
BseBI	CCWGG	1 cut(s)	475
BseDI	CCNNGG	7 cut(s)	18, 19, 89, 90, 176, 320, 420
BseGI	GGATG	2 cut(s)	172, 754
BseJI	GATNNNNATC	1 cut(s)	99
BseLI	CCNNNNNNNGG	5 cut(s)	305, 465, 569, 595, 736
BseMII	CTCAG	3 cut(s)	140, 585, 642
BseNI	ACTGG	1 cut(s)	780
BseRI	GAGGAG	3 cut(s)	680, 683, 686
BseSI	GKGCMC	1 cut(s)	359
BseXI	GCAGC	3 cut(s)	351, 354, 478
BseYI	CCCAGC	1 cut(s)	213
BshFI	GGCC	3 cut(s)	57, 145, 291
BshNI	GGYRCC	1 cut(s)	354
BsiHKAI	GWGCWC	2 cut(s)	386, 473
BsiHKCI	CYCGRG	2 cut(s)	18, 89
BsiSI	CCGG	9 cut(s)	19, 90, 359, 420, 465, 531, 570, 731, 832
BslFI	GGGAC	1 cut(s)	437
BslI	CCNNNNNNNGG	5 cut(s)	305, 465, 569, 595, 736
BsmAI	GTCTC	2 cut(s)	564, 668
BsmBI	CGTCTC	2 cut(s)	564, 668
BsmFI	GGGAC	1 cut(s)	437
BsnI	GGCC	3 cut(s)	57, 145, 291
BsoBI	CYCGRG	2 cut(s)	18, 89
Bsp1286I	GDGCHC	3 cut(s)	359, 386, 473
Bsp143I	GATC	4 cut(s)	94, 250, 679, 726
BspACI	CCGC	4 cut(s)	112, 162, 245, 280
BspANI	GGCC	3 cut(s)	57, 145, 291
BspCNI	CTCAG	3 cut(s)	139, 586, 643
BspHI	TCATGA	2 cut(s)	232, 682
BspLI	GGNNCC	2 cut(s)	356, 425
BspPI	GGATC	4 cut(s)	102, 258, 687, 721
BspT107I	GGYRCC	1 cut(s)	354
BsrI	ACTGG	1 cut(s)	780
BssECI	CCNNGG	7 cut(s)	18, 19, 89, 90, 176, 320, 420
BssMI	GATC	4 cut(s)	94, 250, 679, 726
BssT1I	CCWWGG	1 cut(s)	320
Bst2UI	CCWGG	1 cut(s)	475
Bst4CI	ACNGT	1 cut(s)	586
BstC8I	GCNNGC	2 cut(s)	116, 439
BstDEI	CTNAG	3 cut(s)	126, 594, 651
BstF5I	GGATG	2 cut(s)	172, 754
BstH2I	RGCGCY	1 cut(s)	264
BstHHI	GCGC	1 cut(s)	263
BstKTI	GATC	4 cut(s)	97, 253, 682, 729
BstMAI	GTCTC	2 cut(s)	564, 668
BstMBI	GATC	4 cut(s)	94, 250, 679, 726
BstMWI	GCNNNNNNNGC	1 cut(s)	376
BstNI	CCWGG	1 cut(s)	475
BstNSI	RCATGY	1 cut(s)	745
BstSCI	CCNGG	9 cut(s)	17, 18, 88, 89, 357, 419, 464, 473, 729
BstSFI	CTRYAG	2 cut(s)	331, 606
BstSLI	GKGCMC	1 cut(s)	359
BstV1I	GCAGC	3 cut(s)	351, 354, 478
BstX2I	RGATCY	1 cut(s)	250
BstYI	RGATCY	1 cut(s)	250
BsuRI	GGCC	3 cut(s)	57, 145, 291
BtsCI	GGATG	2 cut(s)	172, 754
BtsI	GCAGTG	1 cut(s)	805
BtsIMutI	CAGTG	1 cut(s)	805
Cac8I	GCNNGC	2 cut(s)	116, 439
CciI	TCATGA	2 cut(s)	232, 682
CfoI	GCGC	1 cut(s)	263
Cfr13I	GGNCC	2 cut(s)	144, 424
Cfr9I	CCCGGG	2 cut(s)	18, 89
Csp6I	GTAC	2 cut(s)	286, 783
CviAII	CATG	5 cut(s)	233, 580, 683, 736, 742
CviQI	GTAC	2 cut(s)	286, 783
DdeI	CTNAG	3 cut(s)	126, 594, 651
DpnI	GATC	4 cut(s)	96, 252, 681, 728
DpnII	GATC	4 cut(s)	94, 250, 679, 726
EaeI	YGGCCR	2 cut(s)	55, 289
EciI	GGCGGA	1 cut(s)	295
Ecl136II	GAGCTC	1 cut(s)	471
Eco130I	CCWWGG	1 cut(s)	320
Eco24I	GRGCYC	1 cut(s)	473
Eco47I	GGWCC	1 cut(s)	424
Eco47III	AGCGCT	1 cut(s)	262
Eco53kI	GAGCTC	1 cut(s)	471
Eco57I	CTGAAG	1 cut(s)	788
Eco88I	CYCGRG	2 cut(s)	18, 89
EcoICRI	GAGCTC	1 cut(s)	471
EcoRI	GAATTC	1 cut(s)	24
EcoRII	CCWGG	1 cut(s)	473
EcoT14I	CCWWGG	1 cut(s)	320
EcoT38I	GRGCYC	1 cut(s)	473
ErhI	CCWWGG	1 cut(s)	320
Esp3I	CGTCTC	2 cut(s)	564, 668
FaeI	CATG	5 cut(s)	236, 583, 686, 739, 745
FaqI	GGGAC	1 cut(s)	437
FatI	CATG	5 cut(s)	232, 579, 682, 735, 741
FauI	CCCGC	2 cut(s)	119, 169
Fnu4HI	GCNGC	3 cut(s)	340, 343, 492
FokI	GGATG	2 cut(s)	159, 761
FriOI	GRGCYC	1 cut(s)	473
Fsp4HI	GCNGC	3 cut(s)	340, 343, 492
FspBI	CTAG	2 cut(s)	36, 786
GlaI	GCGC	1 cut(s)	262
GluI	GCNGC	3 cut(s)	340, 343, 492
GsaI	CCCAGC	1 cut(s)	217
GsuI	CTGGAG	1 cut(s)	457
HaeII	RGCGCY	1 cut(s)	264
HaeIII	GGCC	3 cut(s)	57, 145, 291
HapII	CCGG	9 cut(s)	19, 90, 359, 420, 465, 531, 570, 731, 832
HhaI	GCGC	1 cut(s)	263
Hin1II	CATG	5 cut(s)	236, 583, 686, 739, 745
Hin6I	GCGC	1 cut(s)	261
HinP1I	GCGC	1 cut(s)	261
HinfI	GANTC	5 cut(s)	48, 445, 502, 527, 656
HpaII	CCGG	9 cut(s)	19, 90, 359, 420, 465, 531, 570, 731, 832
HphI	GGTGA	1 cut(s)	7
Hpy188I	TCNGA	3 cut(s)	628, 661, 748
Hpy188III	TCNNGA	2 cut(s)	233, 683
HpyAV	CCTTC	2 cut(s)	360, 763
HpyCH4III	ACNGT	1 cut(s)	586
HpyCH4IV	ACGT	1 cut(s)	720
HpyCH4V	TGCA	3 cut(s)	85, 339, 494
HpyF10VI	GCNNNNNNNGC	1 cut(s)	376
HpyF3I	CTNAG	3 cut(s)	126, 594, 651
HpySE526I	ACGT	1 cut(s)	720
Hsp92II	CATG	5 cut(s)	236, 583, 686, 739, 745
HspAI	GCGC	1 cut(s)	261
Kzo9I	GATC	4 cut(s)	94, 250, 679, 726
LmnI	GCTCC	5 cut(s)	37, 123, 376, 468, 476
Lsp1109I	GCAGC	3 cut(s)	351, 354, 478
LweI	GCATC	2 cut(s)	326, 503
MaeI	CTAG	2 cut(s)	36, 786
MaeII	ACGT	1 cut(s)	720
MaeIII	GTNAC	1 cut(s)	409
MalI	GATC	4 cut(s)	96, 252, 681, 728
MboI	GATC	4 cut(s)	94, 250, 679, 726
MboII	GAAGA	3 cut(s)	95, 213, 394
MflI	RGATCY	1 cut(s)	250
MhlI	GDGCHC	3 cut(s)	359, 386, 473
MlsI	TGGCCA	1 cut(s)	57
MluCI	AATT	4 cut(s)	24, 78, 622, 631
MluNI	TGGCCA	1 cut(s)	57
MlyI	GAGTC	3 cut(s)	439, 511, 521
MmeI	TCCRAC	1 cut(s)	726
Mox20I	TGGCCA	1 cut(s)	57
MscI	TGGCCA	1 cut(s)	57
MslI	CAYNNNNRTG	2 cut(s)	687, 740
Msp20I	TGGCCA	1 cut(s)	57
MspA1I	CMGCKG	2 cut(s)	491, 542
MspI	CCGG	9 cut(s)	19, 90, 359, 420, 465, 531, 570, 731, 832
MspR9I	CCNGG	9 cut(s)	19, 20, 90, 91, 359, 421, 466, 475, 731
MvaI	CCWGG	1 cut(s)	475
MwoI	GCNNNNNNNGC	1 cut(s)	376
NciI	CCSGG	8 cut(s)	19, 20, 90, 91, 359, 421, 466, 731
NdeII	GATC	4 cut(s)	94, 250, 679, 726
NlaIII	CATG	5 cut(s)	236, 583, 686, 739, 745
NlaIV	GGNNCC	2 cut(s)	356, 425
NmeAIII	GCCGAG	1 cut(s)	157
NspI	RCATGY	1 cut(s)	745
PagI	TCATGA	2 cut(s)	232, 682
PciI	ACATGT	1 cut(s)	741
PfeI	GAWTC	2 cut(s)	48, 656
PfoI	TCCNGGA	2 cut(s)	473, 729
PkrI	GCNGC	3 cut(s)	341, 344, 493
PleI	GAGTC	3 cut(s)	439, 510, 521
PpsI	GAGTC	3 cut(s)	439, 510, 521
PscI	ACATGT	1 cut(s)	741
Psp124BI	GAGCTC	1 cut(s)	473
Psp6I	CCWGG	1 cut(s)	473
PspFI	CCCAGC	1 cut(s)	213
PspGI	CCWGG	1 cut(s)	473
PspN4I	GGNNCC	2 cut(s)	356, 425
PspPI	GGNCC	2 cut(s)	144, 424
PsuI	RGATCY	1 cut(s)	250
PvuII	CAGCTG	2 cut(s)	491, 542
RsaI	GTAC	2 cut(s)	287, 784
RsaNI	GTAC	2 cut(s)	286, 783
RseI	CAYNNNNRTG	2 cut(s)	687, 740
SacI	GAGCTC	1 cut(s)	473
SatI	GCNGC	3 cut(s)	340, 343, 492
Sau3AI	GATC	4 cut(s)	94, 250, 679, 726
Sau96I	GGNCC	2 cut(s)	144, 424
SchI	GAGTC	3 cut(s)	439, 511, 521
ScrFI	CCNGG	9 cut(s)	19, 20, 90, 91, 359, 421, 466, 475, 731
SduI	GDGCHC	3 cut(s)	359, 386, 473
SfaNI	GCATC	2 cut(s)	326, 503
SfcI	CTRYAG	2 cut(s)	331, 606
SinI	GGWCC	1 cut(s)	424
SmaI	CCCGGG	2 cut(s)	20, 91
SmiMI	CAYNNNNRTG	2 cut(s)	687, 740
SpeI	ACTAGT	1 cut(s)	785
Sse9I	AATT	4 cut(s)	24, 78, 622, 631
SsiI	CCGC	4 cut(s)	112, 162, 245, 280
SspMI	CTAG	2 cut(s)	36, 786
SstI	GAGCTC	1 cut(s)	473
StyD4I	CCNGG	9 cut(s)	17, 18, 88, 89, 357, 419, 464, 473, 729
StyI	CCWWGG	1 cut(s)	320
TaaI	ACNGT	1 cut(s)	586
TaiI	ACGT	1 cut(s)	723
TaqI	TCGA	4 cut(s)	43, 451, 536, 708
TasI	AATT	4 cut(s)	24, 78, 622, 631
TfiI	GAWTC	2 cut(s)	48, 656
TscAI	CASTG	1 cut(s)	805
TseI	GCWGC	3 cut(s)	339, 342, 491
TspDTI	ATGAA	5 cut(s)	142, 221, 221, 249, 394
TspGWI	ACGGA	1 cut(s)	692
TspMI	CCCGGG	2 cut(s)	18, 89
TspRI	CASTG	1 cut(s)	805
VpaK11BI	GGWCC	1 cut(s)	424
XapI	RAATTY	1 cut(s)	24
XceI	RCATGY	1 cut(s)	745
XmaI	CCCGGG	2 cut(s)	18, 89
XspI	CTAG	2 cut(s)	36, 786

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.