Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004289

Physical Location & Seq

Genomic Coordinates Reverse (-)

30486311 .. 30491344

Gene Structure 5034 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1211300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2505 bp

ATGGAACAGAAGCACATTCTGCTGTCTGCTCTGAGTGTTGGGGTTGGGGTTGGGGTGGGGCTGGGGTTGACTTCCGGGCAGGCTGTGAGCAGTTGGGTAAATGGTAATTGCTCGGCGGATGAGGTGACGGCGGAGCAGATTGAGCAGGAGTTGATGAGGCAGGTTGTGGATGGCAGAGACAGCAAAGTTACATTTGAGGAGTTTCCTTATTACCTAAGAGAGAGAACTCGGATGTTGTTAACAAGCGCTGCATATGTTCATCTGAAGCACTCCGACTTGTCCAAGCACACCCGGAATCTTTCCCCTGCAAGTAGAGCTATTTTGCTCTCAGGACCTGCAGAACTTTATCATCAAATGCTCGCCAAGGCTTTAGCGCATCACTTTGAGTCGAAGTTGCTGTTGTTGGATATCACTGATTTTTCGATCAGGATACAGAGTAAATACGGATGTGCCAAAAGAGAAACACATCATAAAAGGTCCATCTCAGAGGTGACATTGGAGCAAATGTCCAATTTGCTCGGCTCCTTCTCAATGCTCCCTTCAAGCAGGGACACTAAAGGTGCATTATGTCGGCAAAGTAGTTCTTCTGATCTCAAATCAAGGGGCACTGAAGGGCCAACTCGAACGCTTCAAAGAAATGGTTCTTCTGCATCTGATATGAGTAGCATCAGTTCCAAATCTGCTTCGCCAAGTTCAGTTCCTCTCAAACGCGTGAACAACTGGTGTTTCGATGAGAAACTTTTTCTGCACTCACTTTACAAGGTCTTATCTTCAATGTCAGAAACTCGTTCCATCATCTTATGCCTCAGGGACGTCGAGAAGCTTTTTCTCCAATCACGGCGGTTTTACAATTTGTTCAACAAAATGTTGAAGAGACTTTCAGGGTCAGTGTTGATACTTGGTTCCCGGACAGTAGACGCTGAAGATGATTGCAAAGAAGTCGACGAGAAGCTTGCTGCTTTGTTCCCCTACAATATTGAGATCAGTCCCCCGGAAGATGAGACTCATCTCGTCAGCTGGAAGGCTCAACTGGAAGAAGACATGAAGATGATCCAATTTCATGACAACAAAAATCACATAGCCGAGGTGCTTGCGGCGAATGATCTTGAATGTGATGATCTTGGTTCAATCTGCCACGCAGACACAATGGTAGTCAGCAACTACATAGAAGAGATTGTGATTTCGGCAATATCTTATCATTTGATGCAAAACAAGGATCCAGAATATCGAAATGGAAAGCTTGTTATATCATCGACGAGTTTGTCCCATGGATTGAGTATATTCCAGGAGGGAAAATCTGGCGGGAAAGATTCTCTGAAACTGGAGACCAATGCCGATTCCAACAAGGAAACCGAAGGGGAAGAGGCCGTCGGTGCAAAGACTGAATCGAAGTCTGAAACTGCAGCACCGGAAAACAAAGGTGAAGCCGAGAAATCTGGTCCTGGGGTGAAGAAGGACAGCGAGAATCCACCTCCTCCAAAAGTAGAAGTGGTACCTGACAATGAATTTGAGAAGCGCATAAGGCCGGAGGTTATCCCTGCAAATGAGATTGGAGTCACATTTGCAGATATTGGTGCATTGGATGAGATCAAAGAATCACTTCAGGAGTTGGTCATGCTCCCGCTTCGAAGACCAGACCTTTTCAAAGGTGGGCTTCTGAAGCCTTGCAGAGGCATATTGCTTTTCGGTCCTCCTGGCACTGGAAAAACAATGCTGGCAAAGGCCATAGCAAGTGAAGCAGGAGCAAGTTTCATCAACGTCTCGATGTCCACGATCACCTCAAAATGGTTTGGAGAAGATGAGAAGAACGTTCGAGCTCTGTTCACACTTGCAGCAAAGGTCTCCCCGACTATTATCTTCGTGGATGAGGTTGACAGCATGCTGGGGCAGCGAACCAGAGTTGGGGAGCATGAGGCCATGAGGAAGATTAAGAACGAGTTCATGACACACTGGGATGGACTTTTGACAAAGACCGGGGAGAGAATTCTTGTTCTCGCCGCAACCAACAGGCCATTTGACCTTGATGAGGCAATCATCAGGCGATTTGAGCGGAGAGTCATGGTTGGCCTTCCCTCAGTCGAGAACAGGGAAATGATCTTGAGAACTCTTCTTTCGAAAGAAAAGGTCGAAAATCTAGACTTTAAGGAGCTTGCAACCATGACAGAAGGATACAGTGGAAGCGATCTTAAGAACTTGTGCGTGACAGCAGCTTATCGACCAGTTAGGGAGCTTATACAACAGGAGAGGCAAAAGGATGTGGAAAAGAAGAAGAAAGATGCAAAAGAAAAGGGCACAGAAGAAGCTTCAGAACCGAAAGAAGAAGAGAAAGAGGAGCACGTAATTACCCTGAGGGCTTTAAACATGGAAGATATGCGGCAGGCAAAGAATCAGGTTGCTGCGAGTTTCGCTTCAGAGGGATCAGTAATGAGCGAGCTGAAACAGTGGAATGAACTGTATGGCGAGGGTGGGTCGAGGAAGAAGCAACAGCTAACTTATTTCCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0001578 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0006810 GO:0006928 GO:0006996 GO:0006997 GO:0006998 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0008017 GO:0008088 GO:0008089 GO:0008092 GO:0008150 GO:0008568 GO:0009987 GO:0010256 GO:0010458 GO:0010970 GO:0012505 GO:0015631 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019896 GO:0022402 GO:0022411 GO:0022607 GO:0030496 GO:0030705 GO:0031090 GO:0031468 GO:0031965 GO:0031967 GO:0031975 GO:0034214 GO:0034643 GO:0042623 GO:0043014 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0046907 GO:0047497 GO:0048285 GO:0048487 GO:0051013 GO:0051179 GO:0051228 GO:0051230 GO:0051234 GO:0051259 GO:0051260 GO:0051301 GO:0051640 GO:0051641 GO:0051646 GO:0051649 GO:0051654 GO:0051656 GO:0061024 GO:0061640 GO:0065003 GO:0071840 GO:0072384 GO:0090148 GO:0098930 GO:0099111 GO:0140014 GO:0140096 GO:1902850 GO:1903008 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA

Protein Analysis

835

Amino Acids

93.04

Weight (kDa)

5.94

Isoelectric Point (pI)

47.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF7751	PF24933	369 - 429	1.7e-35	Domain of unknown function (DUF7751)
AAA	PF00004	559 - 689	4.6e-39	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	712 - 755	2.3e-14	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004289)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28000 AT4G28000 AT4G28000 AT5G52882
fragaria_vesca	FvH4_7g30620 FvH4_7g30620 FvH4_7g30620 FvH4_7g30620 FvH4_7g30620 FvH4_7g30620 FvH4_7g30620 FvH4_7g30620 FvH4_7g30620
malus_domestica	MD01G1211300.v1.1 MD07G1282000.v1.1
prunus_persica	Prupe.2G303900_v2.0.a1 Prupe.2G303900_v2.0.a1
pyrus_communis	pycom01g22180 pycom07g25590
rosa_chinensis	RchiOBHm_Chr1g0379521
rosa_laevigata	RLG00000026395
rosa_multiflora	Rmu_sc0006761.1_g000024
rosa_roxburghii	Rroxscaffold_4G00280110
rosa_rugosa	Rorug01G0412600 Rorug01G0412700 Rorug01G0412800.1 Rorug01G0412900
rosa_samantha	Rh1AG435400 Rh1BG392800 Rh1CG405400 Rh1DG423600
rosa_wichuraiana	Rw1G038030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1261
AatII	GACGTC	1 cut(s)	816
Acc36I	ACCTGC	2 cut(s)	151, 343
Acc65I	GGTACC	1 cut(s)	1492
AccB1I	GGYRCC	1 cut(s)	1492
AccBSI	CCGCTC	1 cut(s)	2050
AccI	GTMKAC	2 cut(s)	915, 942
AccII	CGCG	1 cut(s)	711
AciI	CCGC	9 cut(s)	116, 131, 841, 1094, 1302, 1622, 1998, 2050, 2374
AclI	AACGTT	1 cut(s)	1809
AclWI	GGATC	4 cut(s)	1045, 1211, 1224, 2425
AcsI	RAATTY	2 cut(s)	1505, 1983
AcuI	CTGAAG	7 cut(s)	284, 630, 942, 1586, 1679, 2289, 2394
AcyI	GRCGYC	1 cut(s)	813
AfaI	GTAC	1 cut(s)	1494
AfeI	AGCGCT	1 cut(s)	247
AfiI	CCNNNNNNNGG	5 cut(s)	1670, 1700, 1785, 1902, 2026
AflII	CTTAAG	1 cut(s)	2186
AflIII	ACRYGT	1 cut(s)	709
AgsI	TTSAA	8 cut(s)	543, 632, 774, 859, 871, 1109, 1128, 1645
AjnI	CCWGG	3 cut(s)	1284, 1441, 1693
Alw21I	GWGCWC	2 cut(s)	1819, 2337
Alw26I	GTCTC	6 cut(s)	171, 868, 995, 1319, 1765, 1846
AlwI	GGATC	4 cut(s)	1045, 1211, 1224, 2425
AlwNI	CAGNNNCTG	1 cut(s)	335
Aor51HI	AGCGCT	1 cut(s)	247
AoxI	GGCC	7 cut(s)	614, 1365, 1523, 1722, 1914, 2009, 2065
ApeKI	GCWGC	7 cut(s)	248, 956, 1403, 1832, 1888, 2207, 2396
ApoI	RAATTY	2 cut(s)	1505, 1983
ArsI	GACNNNNNNTTYG	2 cut(s)	1490, 1522
Asp700I	GAANNNNTTC	3 cut(s)	640, 1599, 1937
Asp718I	GGTACC	1 cut(s)	1492
AspLEI	GCGC	3 cut(s)	248, 376, 1518
AspS9I	GGNCC	5 cut(s)	332, 477, 614, 1439, 1688
AsuC2I	CCSGG	5 cut(s)	76, 292, 907, 992, 1975
AsuHPI	GGTGA	5 cut(s)	136, 502, 1433, 1459, 1768
AsuII	TTCGAA	2 cut(s)	1627, 2114
AvaII	GGWCC	4 cut(s)	332, 477, 1439, 1688
AxyI	CCTNAGG	2 cut(s)	806, 2348
BaeGI	GKGCMC	2 cut(s)	608, 2294
BamHI	GGATCC	1 cut(s)	1216
BanI	GGYRCC	1 cut(s)	1492
BanII	GRGCYC	1 cut(s)	1819
BbsI	GAAGAC	2 cut(s)	1044, 1636
Bbv12I	GWGCWC	2 cut(s)	1819, 2337
BbvI	GCAGC	7 cut(s)	235, 943, 1415, 1844, 1900, 2219, 2383
BccI	CCATC	4 cut(s)	164, 488, 800, 1949
BceAI	ACGGC	3 cut(s)	144, 854, 1352
BcgI	CGANNNNNNTGC	6 cut(s)	922, 935, 956, 969, 1073, 1107
BciT130I	CCWGG	3 cut(s)	1286, 1443, 1695
BciVI	GTATCC	2 cut(s)	423, 2162
BcnI	CCSGG	5 cut(s)	76, 292, 907, 992, 1975
BcoDI	GTCTC	6 cut(s)	171, 868, 995, 1319, 1765, 1846
BfaI	CTAG	1 cut(s)	2135
BfmI	CTRYAG	2 cut(s)	336, 1401
BfoI	RGCGCY	1 cut(s)	249
BfrI	CTTAAG	1 cut(s)	2186
BfuAI	ACCTGC	2 cut(s)	151, 343
BfuI	GTATCC	2 cut(s)	423, 2162
Bme1390I	CCNGG	8 cut(s)	76, 292, 907, 992, 1286, 1443, 1695, 1975
Bme18I	GGWCC	4 cut(s)	332, 477, 1439, 1688
BmgT120I	GGNCC	5 cut(s)	332, 477, 614, 1439, 1688
BmiI	GGNNCC	4 cut(s)	523, 904, 1218, 1494
BmrFI	CCNGG	8 cut(s)	76, 292, 907, 992, 1286, 1443, 1695, 1975
BmrI	ACTGGG	1 cut(s)	1960
BmsI	GCATC	5 cut(s)	385, 659, 675, 1194, 2266
BmuI	ACTGGG	1 cut(s)	1960
BpiI	GAAGAC	2 cut(s)	1044, 1636
BpmI	CTGGAG	1 cut(s)	1343
Bpu14I	TTCGAA	2 cut(s)	1627, 2114
BpuEI	CTTGAG	1 cut(s)	2119
BpuMI	CCSGG	5 cut(s)	76, 292, 907, 992, 1975
BsaAI	YACGTR	1 cut(s)	2338
BsaHI	GRCGYC	1 cut(s)	813
BsaI	GGTCTC	2 cut(s)	1319, 1846
BsaJI	CCNNGG	6 cut(s)	363, 990, 1083, 1267, 1442, 1974
BsaWI	WCCGGW	1 cut(s)	1408
BsaXI	ACNNNNNCTCC	2 cut(s)	491, 521
Bsc4I	CCNNNNNNNGG	5 cut(s)	1670, 1700, 1785, 1902, 2026
Bse1I	ACTGG	6 cut(s)	725, 1035, 1326, 1705, 1955, 2219
Bse21I	CCTNAGG	2 cut(s)	806, 2348
BseBI	CCWGG	3 cut(s)	1286, 1443, 1695
BseDI	CCNNGG	6 cut(s)	363, 990, 1083, 1267, 1442, 1974
BseGI	GGATG	8 cut(s)	124, 175, 237, 452, 1588, 1870, 1960, 2260
BseLI	CCNNNNNNNGG	5 cut(s)	1670, 1700, 1785, 1902, 2026
BseMII	CTCAG	6 cut(s)	23, 342, 498, 820, 2088, 2339
BseNI	ACTGG	6 cut(s)	725, 1035, 1326, 1705, 1955, 2219
BseRI	GAGGAG	3 cut(s)	212, 1464, 2345
BseSI	GKGCMC	2 cut(s)	608, 2294
BseXI	GCAGC	7 cut(s)	235, 943, 1415, 1844, 1900, 2219, 2383
BseYI	CCCAGC	2 cut(s)	61, 1882
BsgI	GTGCAG	1 cut(s)	731
Bsh1236I	CGCG	1 cut(s)	711
BshFI	GGCC	7 cut(s)	616, 1367, 1525, 1724, 1916, 2011, 2067
BshNI	GGYRCC	1 cut(s)	1492
BsiHKAI	GWGCWC	2 cut(s)	1819, 2337
BsiSI	CCGG	7 cut(s)	75, 292, 907, 992, 1409, 1526, 1974
BslFI	GGGAC	4 cut(s)	563, 824, 972, 1249
BslI	CCNNNNNNNGG	5 cut(s)	1670, 1700, 1785, 1902, 2026
BsmAI	GTCTC	6 cut(s)	171, 868, 995, 1319, 1765, 1846
BsmBI	CGTCTC	1 cut(s)	1765
BsmFI	GGGAC	4 cut(s)	563, 824, 972, 1249
BsnI	GGCC	7 cut(s)	616, 1367, 1525, 1724, 1916, 2011, 2067
Bso31I	GGTCTC	2 cut(s)	1319, 1846
Bsp119I	TTCGAA	2 cut(s)	1627, 2114
Bsp1286I	GDGCHC	4 cut(s)	608, 1819, 2294, 2337
Bsp19I	CCATGG	1 cut(s)	1267
BspACI	CCGC	9 cut(s)	116, 131, 841, 1094, 1302, 1622, 1998, 2050, 2374
BspANI	GGCC	7 cut(s)	616, 1367, 1525, 1724, 1916, 2011, 2067
BspCNI	CTCAG	6 cut(s)	24, 341, 497, 819, 2087, 2340
BspFNI	CGCG	1 cut(s)	711
BspHI	TCATGA	2 cut(s)	1060, 1941
BspLI	GGNNCC	4 cut(s)	523, 904, 1218, 1494
BspMAI	CTGCAG	2 cut(s)	340, 1405
BspMI	ACCTGC	2 cut(s)	151, 343
BspPI	GGATC	4 cut(s)	1045, 1211, 1224, 2425
BspT104I	TTCGAA	2 cut(s)	1627, 2114
BspT107I	GGYRCC	1 cut(s)	1492
BspTI	CTTAAG	1 cut(s)	2186
BspTNI	GGTCTC	2 cut(s)	1319, 1846
BsrBI	CCGCTC	1 cut(s)	2050
BsrI	ACTGG	6 cut(s)	725, 1035, 1326, 1705, 1955, 2219
BssECI	CCNNGG	6 cut(s)	363, 990, 1083, 1267, 1442, 1974
BssNI	GRCGYC	1 cut(s)	813
BssT1I	CCWWGG	2 cut(s)	363, 1267
Bst2UI	CCWGG	3 cut(s)	1286, 1443, 1695
Bst4CI	ACNGT	4 cut(s)	913, 2174, 2442, 2454
Bst6I	CTCTTC	5 cut(s)	866, 1164, 1356, 2112, 2316
BstACI	GRCGYC	1 cut(s)	813
BstAFI	CTTAAG	1 cut(s)	2186
BstAPI	GCANNNNNTGC	1 cut(s)	19
BstBAI	YACGTR	1 cut(s)	2338
BstBI	TTCGAA	2 cut(s)	1627, 2114
BstC8I	GCNNGC	9 cut(s)	81, 360, 954, 1092, 1716, 1880, 2151, 2379, 2432
BstDEI	CTNAG	7 cut(s)	32, 215, 328, 484, 806, 2074, 2348
BstDSI	CCRYGG	1 cut(s)	1267
BstENI	CCTNNNNNAGG	2 cut(s)	1668, 2024
BstF5I	GGATG	8 cut(s)	124, 175, 237, 452, 1588, 1870, 1960, 2260
BstFNI	CGCG	1 cut(s)	711
BstH2I	RGCGCY	1 cut(s)	249
BstHHI	GCGC	3 cut(s)	248, 376, 1518
BstMAI	GTCTC	6 cut(s)	171, 868, 995, 1319, 1765, 1846
BstNI	CCWGG	3 cut(s)	1286, 1443, 1695
BstNSI	RCATGY	1 cut(s)	1882
BstSCI	CCNGG	8 cut(s)	74, 290, 905, 990, 1284, 1441, 1693, 1973
BstSFI	CTRYAG	2 cut(s)	336, 1401
BstSLI	GKGCMC	2 cut(s)	608, 2294
BstUI	CGCG	1 cut(s)	711
BstV1I	GCAGC	7 cut(s)	235, 943, 1415, 1844, 1900, 2219, 2383
BstV2I	GAAGAC	2 cut(s)	1044, 1636
BstX2I	RGATCY	1 cut(s)	1216
BstYI	RGATCY	1 cut(s)	1216
Bsu36I	CCTNAGG	2 cut(s)	806, 2348
BsuI	GTATCC	2 cut(s)	423, 2162
BsuRI	GGCC	7 cut(s)	616, 1367, 1525, 1724, 1916, 2011, 2067
BtgI	CCRYGG	1 cut(s)	1267
BtsCI	GGATG	8 cut(s)	124, 175, 237, 452, 1588, 1870, 1960, 2260
BtsIMutI	CAGTG	7 cut(s)	411, 606, 894, 1698, 1948, 2179, 2447
BveI	ACCTGC	2 cut(s)	151, 343
Cac8I	GCNNGC	9 cut(s)	81, 360, 954, 1092, 1716, 1880, 2151, 2379, 2432
CaiI	CAGNNNCTG	1 cut(s)	335
CciI	TCATGA	2 cut(s)	1060, 1941
CfoI	GCGC	3 cut(s)	248, 376, 1518
Cfr13I	GGNCC	5 cut(s)	332, 477, 614, 1439, 1688
CseI	GACGC	1 cut(s)	926
Csp6I	GTAC	1 cut(s)	1493
CviQI	GTAC	1 cut(s)	1493
DdeI	CTNAG	7 cut(s)	32, 215, 328, 484, 806, 2074, 2348
DraI	TTTAAA	1 cut(s)	2358
DrdI	GACNNNNNNGTC	1 cut(s)	1261
DseDI	GACNNNNNNGTC	1 cut(s)	1261
Eam1104I	CTCTTC	5 cut(s)	866, 1164, 1356, 2112, 2316
EarI	CTCTTC	5 cut(s)	866, 1164, 1356, 2112, 2316
EciI	GGCGGA	2 cut(s)	131, 146
Ecl136II	GAGCTC	1 cut(s)	1817
Eco130I	CCWWGG	2 cut(s)	363, 1267
Eco24I	GRGCYC	1 cut(s)	1819
Eco31I	GGTCTC	2 cut(s)	1319, 1846
Eco32I	GATATC	1 cut(s)	409
Eco47I	GGWCC	4 cut(s)	332, 477, 1439, 1688
Eco47III	AGCGCT	1 cut(s)	247
Eco53kI	GAGCTC	1 cut(s)	1817
Eco57I	CTGAAG	7 cut(s)	284, 630, 942, 1586, 1679, 2289, 2394
Eco81I	CCTNAGG	2 cut(s)	806, 2348
EcoICRI	GAGCTC	1 cut(s)	1817
EcoNI	CCTNNNNNAGG	2 cut(s)	1668, 2024
EcoO109I	RGGNCCY	1 cut(s)	332
EcoRI	GAATTC	1 cut(s)	1983
EcoRII	CCWGG	3 cut(s)	1284, 1441, 1693
EcoRV	GATATC	1 cut(s)	409
EcoT14I	CCWWGG	2 cut(s)	363, 1267
EcoT38I	GRGCYC	1 cut(s)	1819
ErhI	CCWWGG	2 cut(s)	363, 1267
Esp3I	CGTCTC	1 cut(s)	1765
FalI	AAGNNNNNCTT	6 cut(s)	568, 600, 1584, 1616, 1638, 1670
FaqI	GGGAC	4 cut(s)	563, 824, 972, 1249
FauI	CCCGC	2 cut(s)	1295, 1629
FauNDI	CATATG	1 cut(s)	253
FblI	GTMKAC	2 cut(s)	915, 942
FokI	GGATG	8 cut(s)	131, 182, 244, 459, 1595, 1877, 1967, 2267
FriOI	GRGCYC	1 cut(s)	1819
FspBI	CTAG	1 cut(s)	2135
GlaI	GCGC	3 cut(s)	247, 375, 1517
GsaI	CCCAGC	2 cut(s)	65, 1886
GsuI	CTGGAG	1 cut(s)	1343
HaeII	RGCGCY	1 cut(s)	249
HaeIII	GGCC	7 cut(s)	616, 1367, 1525, 1724, 1916, 2011, 2067
HapII	CCGG	7 cut(s)	75, 292, 907, 992, 1409, 1526, 1974
HgaI	GACGC	1 cut(s)	926
HhaI	GCGC	3 cut(s)	248, 376, 1518
Hin1I	GRCGYC	1 cut(s)	813
Hin6I	GCGC	3 cut(s)	246, 374, 1516
HinP1I	GCGC	3 cut(s)	246, 374, 1516
HincII	GTYRAC	4 cut(s)	69, 240, 943, 1873
HindII	GTYRAC	4 cut(s)	69, 240, 943, 1873
HindIII	AAGCTT	4 cut(s)	821, 950, 1238, 2301
HpaI	GTTAAC	1 cut(s)	240
HpaII	CCGG	7 cut(s)	75, 292, 907, 992, 1409, 1526, 1974
HphI	GGTGA	5 cut(s)	136, 502, 1433, 1459, 1768
Hpy166II	GTNNAC	8 cut(s)	69, 240, 715, 916, 943, 1770, 1824, 1873
Hpy8I	GTNNAC	8 cut(s)	69, 240, 715, 916, 943, 1770, 1824, 1873
Hpy99I	CGWCG	4 cut(s)	818, 947, 1258, 1373
HpyAV	CCTTC	8 cut(s)	535, 549, 605, 1015, 1349, 1447, 2078, 2159
HpyCH4III	ACNGT	4 cut(s)	913, 2174, 2442, 2454
HpyCH4IV	ACGT	4 cut(s)	813, 1758, 1809, 2337
HpyF3I	CTNAG	7 cut(s)	32, 215, 328, 484, 806, 2074, 2348
HpySE526I	ACGT	4 cut(s)	813, 1758, 1809, 2337
Hsp92I	GRCGYC	1 cut(s)	813
HspAI	GCGC	3 cut(s)	246, 374, 1516
KpnI	GGTACC	1 cut(s)	1496
KspAI	GTTAAC	1 cut(s)	240
Lsp1109I	GCAGC	7 cut(s)	235, 943, 1415, 1844, 1900, 2219, 2383
LweI	GCATC	5 cut(s)	385, 659, 675, 1194, 2266
MaeI	CTAG	1 cut(s)	2135
MaeII	ACGT	4 cut(s)	813, 1758, 1809, 2337
MaeIII	GTNAC	5 cut(s)	124, 187, 490, 1555, 2200
MbiI	CCGCTC	1 cut(s)	2050
MflI	RGATCY	1 cut(s)	1216
MhlI	GDGCHC	4 cut(s)	608, 1819, 2294, 2337
MluCI	AATT	7 cut(s)	106, 511, 850, 1055, 1505, 1983, 2340
MluI	ACGCGT	1 cut(s)	709
MlyI	GAGTC	4 cut(s)	395, 997, 1563, 2064
MmeI	TCCRAC	3 cut(s)	297, 384, 1365
MroXI	GAANNNNTTC	3 cut(s)	640, 1599, 1937
MseI	TTAA	5 cut(s)	239, 1929, 2142, 2187, 2357
MslI	CAYNNNNRTG	2 cut(s)	1046, 1953
MspA1I	CMGCKG	1 cut(s)	1017
MspCI	CTTAAG	1 cut(s)	2186
MspI	CCGG	7 cut(s)	75, 292, 907, 992, 1409, 1526, 1974
MspR9I	CCNGG	8 cut(s)	76, 292, 907, 992, 1286, 1443, 1695, 1975
MvaI	CCWGG	3 cut(s)	1286, 1443, 1695
MvnI	CGCG	1 cut(s)	711
NciI	CCSGG	5 cut(s)	76, 292, 907, 992, 1975
NcoI	CCATGG	1 cut(s)	1267
NdeI	CATATG	1 cut(s)	253
NlaIV	GGNNCC	4 cut(s)	523, 904, 1218, 1494
NmeAIII	GCCGAG	4 cut(s)	92, 498, 1108, 1453
NmuCI	GTSAC	4 cut(s)	124, 490, 1555, 2200
NspI	RCATGY	1 cut(s)	1882
NspV	TTCGAA	2 cut(s)	1627, 2114
PaeI	GCATGC	1 cut(s)	1882
PagI	TCATGA	2 cut(s)	1060, 1941
PcsI	WCGNNNNNNNCGW	1 cut(s)	1769
PdmI	GAANNNNTTC	3 cut(s)	640, 1599, 1937
PfeI	GAWTC	7 cut(s)	295, 1310, 1337, 1385, 1465, 1595, 2386
PfoI	TCCNGGA	2 cut(s)	905, 1284
PleI	GAGTC	4 cut(s)	394, 997, 1562, 2063
PpsI	GAGTC	4 cut(s)	394, 997, 1562, 2063
Ppu21I	YACGTR	1 cut(s)	2338
PpuMI	RGGWCCY	1 cut(s)	332
Psp124BI	GAGCTC	1 cut(s)	1819
Psp1406I	AACGTT	1 cut(s)	1809
Psp5II	RGGWCCY	1 cut(s)	332
Psp6I	CCWGG	3 cut(s)	1284, 1441, 1693
PspFI	CCCAGC	2 cut(s)	61, 1882
PspGI	CCWGG	3 cut(s)	1284, 1441, 1693
PspN4I	GGNNCC	4 cut(s)	523, 904, 1218, 1494
PspPI	GGNCC	5 cut(s)	332, 477, 614, 1439, 1688
PspPPI	RGGWCCY	1 cut(s)	332
PsrI	GAACNNNNNNTAC	2 cut(s)	655, 687
PstI	CTGCAG	2 cut(s)	340, 1405
PstNI	CAGNNNCTG	1 cut(s)	335
PsuI	RGATCY	1 cut(s)	1216
PvuII	CAGCTG	1 cut(s)	1017
RsaI	GTAC	1 cut(s)	1494
RsaNI	GTAC	1 cut(s)	1493
RseI	CAYNNNNRTG	2 cut(s)	1046, 1953
SacI	GAGCTC	1 cut(s)	1819
SalI	GTCGAC	1 cut(s)	941
SaqAI	TTAA	5 cut(s)	239, 1929, 2142, 2187, 2357
Sau96I	GGNCC	5 cut(s)	332, 477, 614, 1439, 1688
SchI	GAGTC	4 cut(s)	395, 997, 1563, 2064
ScrFI	CCNGG	8 cut(s)	76, 292, 907, 992, 1286, 1443, 1695, 1975
SduI	GDGCHC	4 cut(s)	608, 1819, 2294, 2337
SfaNI	GCATC	5 cut(s)	385, 659, 675, 1194, 2266
SfcI	CTRYAG	2 cut(s)	336, 1401
SfuI	TTCGAA	2 cut(s)	1627, 2114
SinI	GGWCC	4 cut(s)	332, 477, 1439, 1688
SmiMI	CAYNNNNRTG	2 cut(s)	1046, 1953
SmlI	CTYRAG	2 cut(s)	2098, 2186
SmoI	CTYRAG	2 cut(s)	2098, 2186
SphI	GCATGC	1 cut(s)	1882
Sse9I	AATT	7 cut(s)	106, 511, 850, 1055, 1505, 1983, 2340
SsiI	CCGC	9 cut(s)	116, 131, 841, 1094, 1302, 1622, 1998, 2050, 2374
SspI	AATATT	1 cut(s)	976
SspMI	CTAG	1 cut(s)	2135
SstI	GAGCTC	1 cut(s)	1819
StyD4I	CCNGG	8 cut(s)	74, 290, 905, 990, 1284, 1441, 1693, 1973
StyI	CCWWGG	2 cut(s)	363, 1267
TaaI	ACNGT	4 cut(s)	913, 2174, 2442, 2454
TaiI	ACGT	4 cut(s)	816, 1761, 1812, 2340
TaqII	GACCGA	1 cut(s)	1676
TasI	AATT	7 cut(s)	106, 511, 850, 1055, 1505, 1983, 2340
TauI	GCSGC	3 cut(s)	1097, 2000, 2377
TfiI	GAWTC	7 cut(s)	295, 1310, 1337, 1385, 1465, 1595, 2386
Tru1I	TTAA	5 cut(s)	239, 1929, 2142, 2187, 2357
Tru9I	TTAA	5 cut(s)	239, 1929, 2142, 2187, 2357
TscAI	CASTG	7 cut(s)	418, 613, 894, 1705, 1955, 2179, 2447
TseFI	GTSAC	4 cut(s)	124, 490, 1555, 2200
TseI	GCWGC	7 cut(s)	248, 956, 1403, 1832, 1888, 2207, 2396
Tsp45I	GTSAC	4 cut(s)	124, 490, 1555, 2200
TspDTI	ATGAA	7 cut(s)	248, 1049, 1058, 1518, 1741, 1930, 2463
TspGWI	ACGGA	1 cut(s)	459
TspRI	CASTG	7 cut(s)	418, 613, 894, 1705, 1955, 2179, 2447
Vha464I	CTTAAG	1 cut(s)	2186
VpaK11BI	GGWCC	4 cut(s)	332, 477, 1439, 1688
XagI	CCTNNNNNAGG	2 cut(s)	1668, 2024
XapI	RAATTY	2 cut(s)	1505, 1983
XbaI	TCTAGA	1 cut(s)	2134
XceI	RCATGY	1 cut(s)	1882
XmiI	GTMKAC	2 cut(s)	915, 942
XmnI	GAANNNNTTC	3 cut(s)	640, 1599, 1937
XspI	CTAG	1 cut(s)	2135
ZraI	GACGTC	1 cut(s)	814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.