Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004558

Physical Location & Seq

Genomic Coordinates Reverse (-)

30747592 .. 30751109

Gene Structure 3518 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1214000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1578 bp

ATGATTGATGCCATAACAAAGTGTGGTCCTGGATTTGTAGGTCCATCTGCAGAAACTTTGAAGACTAAATGGCTGGAAAGTATCAAGTCTGAAATGAGCCTACAGTCAAAAGATATTGAAAAAGAGTGGACTACCACGGGTTGCACCATCATCGCTGACACATGGACTGACAATAAGTCAAGAGCCTTAATCAACTTTTTGGTTTCATCACCCTCACGGACCTTTTTTCACAAGTCGGTGGATGCATCTGCGTATTTCAAGAACACAAAATGCTTGGCGGATTTATTTGATTCTGTTATACAAGATTTTGGCCCGGAAAATGTTGTGCAGATCATCATGGATAGTAGTTTCAACTATACTGGGGTTGCAAACCATATCTTGCAGAATTATTCGACCATTTTCGTGTCTCCTTGTGCCTCTCAATGCTTAAATCTTGTTTTGGAGGAATTCTCGAAGGTAGATTGGGTGAATAGATGCATCTCACAAGCACAAACCATTTCGAAGTTCATCTATAACAATGCCCCGATGCTTGATCTGATGAAAAAGTTTACTGGGGGACAGGAACTCATTAGAACCGGCATCACGAAGTCTGTTTCCAACTTCCTCTCCTTGCAATCTATCTTGAAGCAACGGTCAAGGTTGAAGCACATGTTCAACAGCCCTGAGTATTGCACCAACTCATCTTATGCGAATAAAACACAGAGCATTTCGTGTATTTCCATTGTCGAGGACAATGATTTCTGGAGGGCAGTGGAAGAGAGTGTGGCCATCTCCGAGCCATTTCTCAAAGTGTTGAGGGAAGTGTCTGGAGGAAAACCTGCTGTGGGTTATATATATGAGCTAATGACCAGGGCCAAGGAATCGATAAGGACATACTATATAATGGACGAGAATAAGTGCAAGACCTTCTTAGATATCGTAGACAGAAAGTGGCGGGATCAACTTCATTCGCCTCTACATGCAGCAGCAGCGTTTTTAAACCCCGGCATCCAATACAATCCGGAAATCAAATTTCTTACATCTATTAAGGAGGACTTCTTTAAAGTTCTGGAGAAGCTTCTGCCTGTGCCTGAGATGAGACGCGACATCACGAGTCAAATATTTACATTTACGAAGGCAACTGGGATGTTCGGTTGCAGCCTCGCAATGGAAGCAAGAGATGTAGTTTCACGCCTTTGGTGGGAACAATATGGCGATTCTGCACCCGTGTTGCAACGTGTTGCCATCAGAATACTCAGCCAAGTTTGCAGCACATTCACATTCGAGAGGAACTGGAGCGCATTCCAGCAGATTCACTCGGAGAAGCGCAATAAGATCGATAGAGAAACACTCAACGACCTCGTCTACATAAACTACAACCTCAAGTTAGCAAGGCAGACGAGAACGAAAGCTTTGGAAGCAGATCCGATTCAATTCGACGACATTGACATGACATCGGAGTGGGTGGAGGAGAGTGATAGTCCAAGCCCTACTCAATGGCTTGATCGGTTTGGGTCTGCTCTGGATGGGAGTGACTTGAACACAAGACAGTTTAGTGCTGCCATGTTTGGTTCAAACGACCACATCTTTGGTTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

526

Amino Acids

59.85

Weight (kDa)

5.93

Isoelectric Point (pI)

43.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF659	PF04937	15 - 166	1.9e-59	Domain of unknown function (DUF659)
Dimer_Tnp_hAT	PF05699	393 - 454	1e-14	hAT family C-terminal dimerisation region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004558)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79740 AT1G79740 AT1G79740 AT1G79740
fragaria_vesca	FvH4_5g00491 FvH4_5g00491 FvH4_5g00491 FvH4_7g30891 FvH4_7g30891
malus_domestica	MD01G1214000.v1.1 MD04G1080400.v1.1
pyrus_communis	pycom01g22470 pycom04g07280
rosa_chinensis	RchiOBHm_Chr1g0379971 RchiOBHm_Chr7g0202011
rosa_laevigata	RLG00000026344
rosa_roxburghii	Rroxscaffold_3G00254950
rosa_rugosa	Rorug01G0415900 Rorug01G0415900 Rorug01G0416000.1 Rorug07G0067300.1
rosa_samantha	Rh1AG440700 Rh1BG396400 Rh1CG409600 Rh1DG427200 Rh7AG194500 Rh7BG196000 Rh7CG205800 Rh7DG201200
rosa_wichuraiana	Rw1G038310 Rw7G017040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	826
AccI	GTMKAC	2 cut(s)	921, 1344
AccII	CGCG	1 cut(s)	1083
AccIII	TCCGGA	1 cut(s)	1000
AciI	CCGC	2 cut(s)	278, 934
AclWI	GGATC	2 cut(s)	945, 1397
AcoI	YGGCCR	1 cut(s)	765
AcsI	RAATTY	2 cut(s)	446, 1010
AfiI	CCNNNNNNNGG	3 cut(s)	824, 1147, 1180
AflIII	ACRYGT	2 cut(s)	648, 1216
AhdI	GACNNNNNGTC	1 cut(s)	175
AjnI	CCWGG	2 cut(s)	28, 848
AluBI	AGCT	3 cut(s)	841, 1057, 1391
AluI	AGCT	3 cut(s)	841, 1057, 1391
Alw26I	GTCTC	2 cut(s)	411, 1072
AlwI	GGATC	2 cut(s)	945, 1397
Aor13HI	TCCGGA	1 cut(s)	1000
AoxI	GGCC	3 cut(s)	310, 765, 852
ApeKI	GCWGC	6 cut(s)	962, 965, 968, 1137, 1248, 1538
ApoI	RAATTY	2 cut(s)	446, 1010
ArsI	GACNNNNNNTTYG	1 cut(s)	1550
AspLEI	GCGC	2 cut(s)	1280, 1308
AspS9I	GGNCC	5 cut(s)	26, 41, 219, 311, 852
AsuC2I	CCSGG	2 cut(s)	314, 984
AsuHPI	GGTGA	2 cut(s)	201, 478
AsuII	TTCGAA	1 cut(s)	500
AvaII	GGWCC	3 cut(s)	26, 41, 219
BalI	TGGCCA	1 cut(s)	767
BauI	CACGAG	1 cut(s)	1090
BbsI	GAAGAC	1 cut(s)	68
BbvI	GCAGC	6 cut(s)	974, 977, 980, 1149, 1260, 1525
BccI	CCATC	5 cut(s)	52, 155, 776, 1232, 1499
BcgI	CGANNNNNNTGC	4 cut(s)	133, 167, 1297, 1331
BciT130I	CCWGG	2 cut(s)	30, 850
BcnI	CCSGG	2 cut(s)	314, 984
BcoDI	GTCTC	2 cut(s)	411, 1072
BfmI	CTRYAG	2 cut(s)	48, 101
BfuAI	ACCTGC	1 cut(s)	826
BisI	GCNGC	6 cut(s)	963, 966, 969, 1138, 1249, 1539
BlsI	GCNGC	6 cut(s)	964, 967, 970, 1139, 1250, 1540
Bme1390I	CCNGG	4 cut(s)	30, 314, 850, 984
Bme18I	GGWCC	3 cut(s)	26, 41, 219
BmeRI	GACNNNNNGTC	1 cut(s)	175
BmgT120I	GGNCC	5 cut(s)	26, 41, 219, 311, 852
BmrFI	CCNGG	4 cut(s)	30, 314, 850, 984
BmrI	ACTGGG	3 cut(s)	369, 561, 1131
BmsI	GCATC	7 cut(s)	232, 254, 464, 486, 516, 588, 996
BmuI	ACTGGG	3 cut(s)	369, 561, 1131
BpiI	GAAGAC	1 cut(s)	68
BplI	GAGNNNNNCTC	4 cut(s)	434, 466, 1314, 1346
BpmI	CTGGAG	4 cut(s)	763, 828, 1070, 1294
Bpu14I	TTCGAA	1 cut(s)	500
BpuEI	CTTGAG	1 cut(s)	1346
BpuMI	CCSGG	2 cut(s)	314, 984
Bsa29I	ATCGAT	2 cut(s)	863, 1317
BsaJI	CCNNGG	4 cut(s)	135, 849, 855, 982
BsaWI	WCCGGW	1 cut(s)	1000
BsaXI	ACNNNNNCTCC	2 cut(s)	590, 620
Bsc4I	CCNNNNNNNGG	3 cut(s)	824, 1147, 1180
Bse118I	RCCGGY	1 cut(s)	575
Bse1I	ACTGG	4 cut(s)	364, 556, 1126, 1277
Bse3DI	GCAATG	1 cut(s)	1152
BseAI	TCCGGA	1 cut(s)	1000
BseBI	CCWGG	2 cut(s)	30, 850
BseCI	ATCGAT	2 cut(s)	863, 1317
BseDI	CCNNGG	4 cut(s)	135, 849, 855, 982
BseGI	GGATG	4 cut(s)	247, 987, 1131, 1510
BseLI	CCNNNNNNNGG	3 cut(s)	824, 1147, 1180
BseMI	GCAATG	1 cut(s)	1152
BseMII	CTCAG	3 cut(s)	654, 1062, 1249
BseNI	ACTGG	4 cut(s)	364, 556, 1126, 1277
BseRI	GAGGAG	1 cut(s)	1463
BseXI	GCAGC	6 cut(s)	974, 977, 980, 1149, 1260, 1525
BsgI	GTGCAG	2 cut(s)	347, 1185
Bsh1236I	CGCG	1 cut(s)	1083
BshFI	GGCC	3 cut(s)	312, 767, 854
BshVI	ATCGAT	2 cut(s)	863, 1317
BsiSI	CCGG	4 cut(s)	314, 576, 984, 1001
BslFI	GGGAC	1 cut(s)	570
BslI	CCNNNNNNNGG	3 cut(s)	824, 1147, 1180
BsmAI	GTCTC	2 cut(s)	411, 1072
BsmBI	CGTCTC	1 cut(s)	1072
BsmFI	GGGAC	1 cut(s)	570
BsmI	GAATGC	1 cut(s)	1280
BsnI	GGCC	3 cut(s)	312, 767, 854
Bsp119I	TTCGAA	1 cut(s)	500
Bsp13I	TCCGGA	1 cut(s)	1000
Bsp143I	GATC	6 cut(s)	330, 532, 937, 1314, 1402, 1483
BspACI	CCGC	2 cut(s)	278, 934
BspANI	GGCC	3 cut(s)	312, 767, 854
BspCNI	CTCAG	3 cut(s)	655, 1063, 1248
BspDI	ATCGAT	2 cut(s)	863, 1317
BspEI	TCCGGA	1 cut(s)	1000
BspFNI	CGCG	1 cut(s)	1083
BspMAI	CTGCAG	1 cut(s)	52
BspMI	ACCTGC	1 cut(s)	826
BspPI	GGATC	2 cut(s)	945, 1397
BspT104I	TTCGAA	1 cut(s)	500
BsrDI	GCAATG	1 cut(s)	1152
BsrFI	RCCGGY	1 cut(s)	575
BsrI	ACTGG	4 cut(s)	364, 556, 1126, 1277
BssAI	RCCGGY	1 cut(s)	575
BssECI	CCNNGG	4 cut(s)	135, 849, 855, 982
BssMI	GATC	6 cut(s)	330, 532, 937, 1314, 1402, 1483
BssSI	CACGAG	1 cut(s)	1090
BssT1I	CCWWGG	1 cut(s)	855
Bst2BI	CACGAG	1 cut(s)	1090
Bst2UI	CCWGG	2 cut(s)	30, 850
Bst4CI	ACNGT	3 cut(s)	105, 633, 1530
Bst6I	CTCTTC	1 cut(s)	750
BstBI	TTCGAA	1 cut(s)	500
BstDEI	CTNAG	4 cut(s)	663, 910, 1071, 1235
BstDSI	CCRYGG	1 cut(s)	135
BstF5I	GGATG	4 cut(s)	247, 987, 1131, 1510
BstFNI	CGCG	1 cut(s)	1083
BstHHI	GCGC	2 cut(s)	1280, 1308
BstKTI	GATC	6 cut(s)	333, 535, 940, 1317, 1405, 1486
BstMAI	GTCTC	2 cut(s)	411, 1072
BstMBI	GATC	6 cut(s)	330, 532, 937, 1314, 1402, 1483
BstMWI	GCNNNNNNNGC	4 cut(s)	968, 1151, 1245, 1397
BstNI	CCWGG	2 cut(s)	30, 850
BstNSI	RCATGY	2 cut(s)	652, 962
BstSCI	CCNGG	4 cut(s)	28, 312, 848, 982
BstSFI	CTRYAG	2 cut(s)	48, 101
BstUI	CGCG	1 cut(s)	1083
BstV1I	GCAGC	6 cut(s)	974, 977, 980, 1149, 1260, 1525
BstV2I	GAAGAC	1 cut(s)	68
BstX2I	RGATCY	1 cut(s)	1402
BstXI	CCANNNNNNTGG	1 cut(s)	1568
BstYI	RGATCY	1 cut(s)	1402
Bsu15I	ATCGAT	2 cut(s)	863, 1317
BsuRI	GGCC	3 cut(s)	312, 767, 854
BsuTUI	ATCGAT	2 cut(s)	863, 1317
BtgI	CCRYGG	1 cut(s)	135
BtgZI	GCGATG	1 cut(s)	136
BtsCI	GGATG	4 cut(s)	247, 987, 1131, 1510
BtsI	GCAGTG	1 cut(s)	756
BtsIMutI	CAGTG	1 cut(s)	756
BveI	ACCTGC	1 cut(s)	826
CfoI	GCGC	2 cut(s)	1280, 1308
Cfr10I	RCCGGY	1 cut(s)	575
Cfr13I	GGNCC	5 cut(s)	26, 41, 219, 311, 852
ClaI	ATCGAT	2 cut(s)	863, 1317
CseI	GACGC	1 cut(s)	1089
CviAII	CATG	6 cut(s)	162, 337, 649, 959, 1429, 1543
DdeI	CTNAG	4 cut(s)	663, 910, 1071, 1235
DpnI	GATC	6 cut(s)	332, 534, 939, 1316, 1404, 1485
DpnII	GATC	6 cut(s)	330, 532, 937, 1314, 1402, 1483
DraI	TTTAAA	2 cut(s)	978, 1042
DriI	GACNNNNNGTC	1 cut(s)	175
EaeI	YGGCCR	1 cut(s)	765
Eam1104I	CTCTTC	1 cut(s)	750
Eam1105I	GACNNNNNGTC	1 cut(s)	175
EarI	CTCTTC	1 cut(s)	750
EciI	GGCGGA	1 cut(s)	293
Eco130I	CCWWGG	1 cut(s)	855
Eco32I	GATATC	1 cut(s)	916
Eco47I	GGWCC	3 cut(s)	26, 41, 219
EcoRI	GAATTC	1 cut(s)	446
EcoRII	CCWGG	2 cut(s)	28, 848
EcoRV	GATATC	1 cut(s)	916
EcoT14I	CCWWGG	1 cut(s)	855
EcoT22I	ATGCAT	2 cut(s)	247, 479
ErhI	CCWWGG	1 cut(s)	855
Esp3I	CGTCTC	1 cut(s)	1072
FaeI	CATG	6 cut(s)	165, 340, 652, 962, 1432, 1546
FalI	AAGNNNNNCTT	4 cut(s)	893, 925, 1019, 1051
FaqI	GGGAC	1 cut(s)	570
FatI	CATG	6 cut(s)	161, 336, 648, 958, 1428, 1542
FauI	CCCGC	1 cut(s)	927
FblI	GTMKAC	2 cut(s)	921, 1344
Fnu4HI	GCNGC	6 cut(s)	963, 966, 969, 1138, 1249, 1539
FokI	GGATG	4 cut(s)	254, 974, 1138, 1517
Fsp4HI	GCNGC	6 cut(s)	963, 966, 969, 1138, 1249, 1539
GlaI	GCGC	2 cut(s)	1279, 1307
GluI	GCNGC	6 cut(s)	963, 966, 969, 1138, 1249, 1539
GsuI	CTGGAG	4 cut(s)	763, 828, 1070, 1294
HaeIII	GGCC	3 cut(s)	312, 767, 854
HapII	CCGG	4 cut(s)	314, 576, 984, 1001
HgaI	GACGC	1 cut(s)	1089
HhaI	GCGC	2 cut(s)	1280, 1308
Hin1II	CATG	6 cut(s)	165, 340, 652, 962, 1432, 1546
Hin6I	GCGC	2 cut(s)	1278, 1306
HinP1I	GCGC	2 cut(s)	1278, 1306
HindIII	AAGCTT	2 cut(s)	1055, 1389
HinfI	GANTC	6 cut(s)	290, 860, 1093, 1196, 1291, 1408
HpaII	CCGG	4 cut(s)	314, 576, 984, 1001
HphI	GGTGA	2 cut(s)	201, 478
Hpy166II	GTNNAC	4 cut(s)	129, 549, 922, 1345
Hpy188I	TCNGA	7 cut(s)	91, 537, 775, 1229, 1300, 1407, 1438
Hpy8I	GTNNAC	4 cut(s)	129, 549, 922, 1345
Hpy99I	CGWCG	1 cut(s)	1421
HpyAV	CCTTC	3 cut(s)	448, 916, 1108
HpyCH4III	ACNGT	3 cut(s)	105, 633, 1530
HpyCH4IV	ACGT	1 cut(s)	1216
HpyF10VI	GCNNNNNNNGC	4 cut(s)	968, 1151, 1245, 1397
HpyF3I	CTNAG	4 cut(s)	663, 910, 1071, 1235
HpySE526I	ACGT	1 cut(s)	1216
Hsp92II	CATG	6 cut(s)	165, 340, 652, 962, 1432, 1546
HspAI	GCGC	2 cut(s)	1278, 1306
Kpn2I	TCCGGA	1 cut(s)	1000
Kzo9I	GATC	6 cut(s)	330, 532, 937, 1314, 1402, 1483
LmnI	GCTCC	1 cut(s)	1275
Lsp1109I	GCAGC	6 cut(s)	974, 977, 980, 1149, 1260, 1525
LweI	GCATC	7 cut(s)	232, 254, 464, 486, 516, 588, 996
MaeII	ACGT	1 cut(s)	1216
MaeIII	GTNAC	1 cut(s)	1511
MalI	GATC	6 cut(s)	332, 534, 939, 1316, 1404, 1485
MboI	GATC	6 cut(s)	330, 532, 937, 1314, 1402, 1483
MboII	GAAGA	2 cut(s)	73, 767
MflI	RGATCY	1 cut(s)	1402
MlsI	TGGCCA	1 cut(s)	767
MluCI	AATT	4 cut(s)	385, 446, 1010, 1412
MluNI	TGGCCA	1 cut(s)	767
MlyI	GAGTC	1 cut(s)	1102
MmeI	TCCRAC	1 cut(s)	621
Mox20I	TGGCCA	1 cut(s)	767
Mph1103I	ATGCAT	2 cut(s)	247, 479
MroI	TCCGGA	1 cut(s)	1000
MscI	TGGCCA	1 cut(s)	767
MseI	TTAA	5 cut(s)	188, 428, 977, 1026, 1041
MslI	CAYNNNNRTG	3 cut(s)	401, 1427, 1438
Msp20I	TGGCCA	1 cut(s)	767
MspI	CCGG	4 cut(s)	314, 576, 984, 1001
MspR9I	CCNGG	4 cut(s)	30, 314, 850, 984
Mva1269I	GAATGC	1 cut(s)	1280
MvaI	CCWGG	2 cut(s)	30, 850
MvnI	CGCG	1 cut(s)	1083
MwoI	GCNNNNNNNGC	4 cut(s)	968, 1151, 1245, 1397
NciI	CCSGG	2 cut(s)	314, 984
NdeII	GATC	6 cut(s)	330, 532, 937, 1314, 1402, 1483
NlaIII	CATG	6 cut(s)	165, 340, 652, 962, 1432, 1546
NmuCI	GTSAC	1 cut(s)	1511
NsiI	ATGCAT	2 cut(s)	247, 479
NspI	RCATGY	2 cut(s)	652, 962
NspV	TTCGAA	1 cut(s)	500
PciI	ACATGT	1 cut(s)	648
PctI	GAATGC	1 cut(s)	1280
PfeI	GAWTC	5 cut(s)	290, 860, 1196, 1291, 1408
PflFI	GACNNNGTC	1 cut(s)	1340
PfoI	TCCNGGA	1 cut(s)	28
PkrI	GCNGC	6 cut(s)	964, 967, 970, 1139, 1250, 1540
PleI	GAGTC	1 cut(s)	1101
PpsI	GAGTC	1 cut(s)	1101
PscI	ACATGT	1 cut(s)	648
Psp6I	CCWGG	2 cut(s)	28, 848
PspGI	CCWGG	2 cut(s)	28, 848
PspPI	GGNCC	5 cut(s)	26, 41, 219, 311, 852
PstI	CTGCAG	1 cut(s)	52
PsuI	RGATCY	1 cut(s)	1402
PsyI	GACNNNGTC	1 cut(s)	1340
RseI	CAYNNNNRTG	3 cut(s)	401, 1427, 1438
SaqAI	TTAA	5 cut(s)	188, 428, 977, 1026, 1041
SatI	GCNGC	6 cut(s)	963, 966, 969, 1138, 1249, 1539
Sau3AI	GATC	6 cut(s)	330, 532, 937, 1314, 1402, 1483
Sau96I	GGNCC	5 cut(s)	26, 41, 219, 311, 852
SchI	GAGTC	1 cut(s)	1102
ScrFI	CCNGG	4 cut(s)	30, 314, 850, 984
SfaNI	GCATC	7 cut(s)	232, 254, 464, 486, 516, 588, 996
SfcI	CTRYAG	2 cut(s)	48, 101
SfuI	TTCGAA	1 cut(s)	500
SinI	GGWCC	3 cut(s)	26, 41, 219
SmiMI	CAYNNNNRTG	3 cut(s)	401, 1427, 1438
SmlI	CTYRAG	1 cut(s)	1361
SmoI	CTYRAG	1 cut(s)	1361
Sse9I	AATT	4 cut(s)	385, 446, 1010, 1412
SsiI	CCGC	2 cut(s)	278, 934
SspI	AATATT	1 cut(s)	1101
StyD4I	CCNGG	4 cut(s)	28, 312, 848, 982
StyI	CCWWGG	1 cut(s)	855
TaaI	ACNGT	3 cut(s)	105, 633, 1530
TaiI	ACGT	1 cut(s)	1219
TaqI	TCGA	8 cut(s)	392, 452, 500, 726, 863, 1263, 1317, 1416
TasI	AATT	4 cut(s)	385, 446, 1010, 1412
TfiI	GAWTC	5 cut(s)	290, 860, 1196, 1291, 1408
Tru1I	TTAA	5 cut(s)	188, 428, 977, 1026, 1041
Tru9I	TTAA	5 cut(s)	188, 428, 977, 1026, 1041
TscAI	CASTG	1 cut(s)	756
TseFI	GTSAC	1 cut(s)	1511
TseI	GCWGC	6 cut(s)	962, 965, 968, 1137, 1248, 1538
Tsp45I	GTSAC	1 cut(s)	1511
TspDTI	ATGAA	4 cut(s)	195, 496, 554, 935
TspGWI	ACGGA	1 cut(s)	232
TspRI	CASTG	1 cut(s)	756
Tth111I	GACNNNGTC	1 cut(s)	1340
VpaK11BI	GGWCC	3 cut(s)	26, 41, 219
XapI	RAATTY	2 cut(s)	446, 1010
XceI	RCATGY	2 cut(s)	652, 962
XmiI	GTMKAC	2 cut(s)	921, 1344
Zsp2I	ATGCAT	2 cut(s)	247, 479

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.