MD01G1222800.v1.1

Tetratricopeptide repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009192
Physical Location & Seq
Reverse (-)
31489223 .. 31492871
3649 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1222800.v1.1.491

Sequence Viewer

Length: 1719 bp
ATGGCAGCTTCCCTTCTTCTCTCTCCTCCCTTCTACAACAACAACGGGAAGAGCGAAGTGTGCTTGCATCGCATGATGCAGTCTGTCCATTTGCACAAAGGCAGCAATTTCTTCTCTGTTTGTCTCCCGAAAGCGAATTGCAGCTTCAAGCTTTTCATGGTACCTGTAAGAAACTTTGACCTCTGTTGTGTCTCCAGAACCCTTGCTTCTGTTGGCTCATTGGATAGAAATCTGGCAGCCCTACAAAAGCACTCTCAGAGGTTCAAAACTTCTTTTAAGCAATCAAGTGATAAATTAGACGGGATGAAAAGTTTTGAAAGAGAACTGCAAGAGTTATTTGATGAAGTCAAAAGGATGATTATGATGGGGAACAAAAATGATGCCATAGACCTACTTCAGGCCAATTATGAAGCTGTGAAGGAACGGATGGATGCCGGCATTAGAGGCATTGAAGAAGCTGCCACCATTGACATCATAGCTCTAGGATACATGGCTATTGGCGACTTGAAATTTGTTGGTTCCCTACTGGATATGTTGAATGAAGTTGTTGGCAGTCTAAAGGATGATGAACCGCTTCTGGATTCAGTTCTCGTGCATATGGGGAGCATGTATTCAACTTTGGGAGAGTTTGAGAAATCAATGTCTGCATATAGGAGGGCTATTGGTAACATGGAGAACATATATGGGAAAAATAGTGTTTTTCTTATCACACCAATTTTGGGGATTGCAAAAGTTCTCACCTCCATCGGAAGAACTTCAAAAGCAGTAGAATTCTACAACCGTGCAATTACTCTTCTAGAATCAAGCAGAGGTGCTGAAAGTGAGGATTTGGTCATACCTTTATTTGGCCTTGGCAATCTTTTGCTCAAAGAAGGAAAGCCTACCGAGGCAGAAACTCCTTTTCTAAGAGTTTTGAACATATACAAAAAATTATATGGAGAAAATGATGGACGAGTTGGAGTGGCTATGTGCTCCCTGGCCCATGTGAAGTGTGCCATGGGAAATGCAAATGAAGCCATCTCTTTATATAGAAAAGCGCTCGAAGTGATCACGTCTTCCAATTATATGGCCTTAGATGACAGCATAATGGAGAAGATGAGGATAGATTTGGCAGAGTTACTTCATGCTGTTGGAAGAGGAAAGGAAGGCCGAGAGCTGCTGGAGGAATGTCTGATGATCACAGAGAGATATAAAGGTAAAGAGGACCCCAGCTCAGCGACACACCTTATTAATCTTGCAACTTCGCATTCACGGTCAAAGAATTATGCGGAGGCTGAGCGCTTGCTGAGGACAAGTTTGCAGATCATGGAAAAGACAGTGGGGCCTGATGATCAATCCATCACGTTCCCAATGCTGCACCTCGCCGTTACACTCTACCATCTGAAACGGGATGAAGAAGCTGAACAGCTTGCCCTGGAGGCTTTGCGCATTCGTGAAAAAGCATTTGGAAACGATTCCCTTCCTGTTGCGGAGGCTCTTGACTGTTTAGTGTCGATTCAAACCAGATTAACGAAGGACAACGAATCGTTACTCGAGCAGCTGAAGAGGGTTTTGAGCATCCAAGAGAAGGAGTTTGGTGCCGAGAGCGAGGAGGTAATGATCACCCTGAAAAAACTTGTATTCTACTTGGAGAAACTAGGGAGGAAAAACGACGTTTTCCCTTTGCAGAAAAGATTGTCTGCACTTCGATTGAAATTTAAGCAACGGATTCAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_10 TPR_12 TPR_8

Protein Analysis

573

Amino Acids

64.22

Weight (kDa)

6.89

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 195 - 267 3.8e-08 Tetratricopeptide repeat
TPR_MalT PF17874 196 - 375 2.3e-14 MalT-like TPR region
TPR_12 PF13424 283 - 349 7e-11 Tetratricopeptide repeat
TPR_12 PF13424 368 - 438 1.2e-12 Tetratricopeptide repeat
TPR_NPHP3 PF24885 373 - 480 5.9e-07 Nephrocystin-3 TPR domain
TPR_10 PF13374 406 - 444 8.8e-08 Tetratricopeptide repeat
TPR_NPHP3 PF24885 426 - 546 5.3e-08 Nephrocystin-3 TPR domain
TPR_10 PF13374 451 - 486 8.4e-08 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1427
Acc65I GGTACC 1 cut(s) 160
AccB1I GGYRCC 2 cut(s) 160, 1577
AciI CCGC 3 cut(s) 572, 1268, 1469
AcsI RAATTY 3 cut(s) 509, 770, 1694
AcuI CTGAAG 2 cut(s) 380, 1562
AfaI GTAC 1 cut(s) 162
AfeI AGCGCT 2 cut(s) 1038, 1280
AfiI CCNNNNNNNGG 4 cut(s) 397, 719, 845, 1567
AjiI CACGTC 1 cut(s) 1053
AjnI CCWGG 2 cut(s) 975, 1413
AjuI GAANNNNNNNTTGG 2 cut(s) 1428, 1460
Alw21I GWGCWC 1 cut(s) 974
Alw26I GTCTC 2 cut(s) 128, 196
Ama87I CYCGRG 1 cut(s) 1532
Aor51HI AGCGCT 2 cut(s) 1038, 1280
AoxI GGCC 6 cut(s) 399, 847, 978, 1068, 1147, 1322
ApeKI GCWGC 8 cut(s) 5, 102, 141, 236, 458, 1156, 1354, 1537
ApoI RAATTY 3 cut(s) 509, 770, 1694
ArsI GACNNNNNNTTYG 2 cut(s) 494, 526
AseI ATTAAT 1 cut(s) 1230
Asp700I GAANNNNTTC 3 cut(s) 573, 754, 1453
Asp718I GGTACC 1 cut(s) 160
AspLEI GCGC 3 cut(s) 1039, 1281, 1428
AspS9I GGNCC 3 cut(s) 979, 1204, 1322
AsuHPI GGTGA 2 cut(s) 730, 1594
AvaI CYCGRG 1 cut(s) 1532
AvaII GGWCC 1 cut(s) 1204
BanI GGYRCC 2 cut(s) 160, 1577
BauI CACGAG 1 cut(s) 590
BbsI GAAGAC 1 cut(s) 1047
Bbv12I GWGCWC 1 cut(s) 974
BbvCI CCTCAGC 1 cut(s) 1286
BbvI GCAGC 8 cut(s) 17, 114, 153, 248, 445, 1143, 1341, 1549
BccI CCATC 7 cut(s) 358, 421, 752, 941, 1025, 1346, 1386
BceAI ACGGC 1 cut(s) 1349
BciT130I CCWGG 2 cut(s) 977, 1415
BciVI GTATCC 1 cut(s) 479
BclI TGATCA 4 cut(s) 1047, 1176, 1330, 1599
BcoDI GTCTC 2 cut(s) 128, 196
BfaI CTAG 3 cut(s) 482, 797, 1637
BfoI RGCGCY 2 cut(s) 1040, 1282
BfuI GTATCC 1 cut(s) 479
BglI GCCNNNNNGGC 1 cut(s) 1418
BisI GCNGC 8 cut(s) 6, 103, 142, 237, 459, 1157, 1355, 1538
BlpI GCTNAGC 2 cut(s) 1213, 1275
BlsI GCNGC 8 cut(s) 7, 104, 143, 238, 460, 1158, 1356, 1539
Bme1390I CCNGG 2 cut(s) 977, 1415
Bme18I GGWCC 1 cut(s) 1204
BmeT110I CYCGRG 1 cut(s) 1532
BmgBI CACGTC 1 cut(s) 1053
BmgT120I GGNCC 3 cut(s) 979, 1204, 1322
BmiI GGNNCC 5 cut(s) 162, 520, 1206, 1323, 1579
BmrFI CCNGG 2 cut(s) 977, 1415
BmsI GCATC 5 cut(s) 66, 76, 370, 421, 1566
BpiI GAAGAC 1 cut(s) 1047
BpmI CTGGAG 3 cut(s) 178, 1181, 1436
Bpu10I CCTNAGC 1 cut(s) 1286
Bpu1102I GCTNAGC 2 cut(s) 1213, 1275
BsaBI GATNNNNATC 1 cut(s) 228
BsaJI CCNNGG 5 cut(s) 850, 885, 975, 996, 1413
Bsc4I CCNNNNNNNGG 4 cut(s) 397, 719, 845, 1567
Bse118I RCCGGY 1 cut(s) 434
Bse1I ACTGG 1 cut(s) 531
Bse8I GATNNNNATC 1 cut(s) 228
BseBI CCWGG 2 cut(s) 977, 1415
BseDI CCNNGG 5 cut(s) 850, 885, 975, 996, 1413
BseGI GGATG 7 cut(s) 309, 360, 432, 436, 568, 1396, 1557
BseJI GATNNNNATC 1 cut(s) 228
BseLI CCNNNNNNNGG 4 cut(s) 397, 719, 845, 1567
BseMII CTCAG 4 cut(s) 269, 1227, 1266, 1277
BseNI ACTGG 1 cut(s) 531
BseRI GAGGAG 2 cut(s) 15, 1604
BseXI GCAGC 8 cut(s) 17, 114, 153, 248, 445, 1143, 1341, 1549
BseYI CCCAGC 1 cut(s) 1208
BsgI GTGCAG 2 cut(s) 1340, 1665
BshFI GGCC 6 cut(s) 401, 849, 980, 1070, 1149, 1324
BshNI GGYRCC 2 cut(s) 160, 1577
BsiHKAI GWGCWC 1 cut(s) 974
BsiHKCI CYCGRG 1 cut(s) 1532
BsiSI CCGG 1 cut(s) 435
BslI CCNNNNNNNGG 4 cut(s) 397, 719, 845, 1567
BsmAI GTCTC 2 cut(s) 128, 196
BsmI GAATGC 2 cut(s) 1246, 1428
BsnI GGCC 6 cut(s) 401, 849, 980, 1070, 1149, 1324
BsoBI CYCGRG 1 cut(s) 1532
Bsp1286I GDGCHC 1 cut(s) 974
Bsp143I GATC 5 cut(s) 1047, 1176, 1302, 1330, 1599
Bsp1720I GCTNAGC 2 cut(s) 1213, 1275
Bsp19I CCATGG 1 cut(s) 996
BspACI CCGC 3 cut(s) 572, 1268, 1469
BspANI GGCC 6 cut(s) 401, 849, 980, 1070, 1149, 1324
BspCNI CTCAG 4 cut(s) 268, 1226, 1267, 1278
BspLI GGNNCC 5 cut(s) 162, 520, 1206, 1323, 1579
BspQI GCTCTTC 1 cut(s) 44
BspT107I GGYRCC 2 cut(s) 160, 1577
BsrFI RCCGGY 1 cut(s) 434
BsrI ACTGG 1 cut(s) 531
BssAI RCCGGY 1 cut(s) 434
BssECI CCNNGG 5 cut(s) 850, 885, 975, 996, 1413
BssMI GATC 5 cut(s) 1047, 1176, 1302, 1330, 1599
BssSI CACGAG 1 cut(s) 590
BssT1I CCWWGG 2 cut(s) 850, 996
Bst2BI CACGAG 1 cut(s) 590
Bst2UI CCWGG 2 cut(s) 977, 1415
Bst4CI ACNGT 4 cut(s) 782, 1254, 1318, 1484
Bst6I CTCTTC 4 cut(s) 44, 798, 1129, 1538
BstC8I GCNNGC 4 cut(s) 65, 436, 1283, 1410
BstDEI CTNAG 6 cut(s) 255, 905, 1072, 1213, 1275, 1286
BstDSI CCRYGG 1 cut(s) 996
BstENI CCTNNNNNAGG 1 cut(s) 395
BstF5I GGATG 7 cut(s) 309, 360, 432, 436, 568, 1396, 1557
BstH2I RGCGCY 2 cut(s) 1040, 1282
BstHHI GCGC 3 cut(s) 1039, 1281, 1428
BstKTI GATC 5 cut(s) 1050, 1179, 1305, 1333, 1602
BstMAI GTCTC 2 cut(s) 128, 196
BstMBI GATC 5 cut(s) 1047, 1176, 1302, 1330, 1599
BstMWI GCNNNNNNNGC 5 cut(s) 60, 69, 444, 1013, 1418
BstNI CCWGG 2 cut(s) 977, 1415
BstNSI RCATGY 1 cut(s) 610
BstSCI CCNGG 2 cut(s) 975, 1413
BstV1I GCAGC 8 cut(s) 17, 114, 153, 248, 445, 1143, 1341, 1549
BstV2I GAAGAC 1 cut(s) 1047
BstXI CCANNNNNNTGG 1 cut(s) 1066
BsuI GTATCC 1 cut(s) 479
BsuRI GGCC 6 cut(s) 401, 849, 980, 1070, 1149, 1324
BtgI CCRYGG 1 cut(s) 996
BtgZI GCGATG 1 cut(s) 53
BtrI CACGTC 1 cut(s) 1053
BtsCI GGATG 7 cut(s) 309, 360, 432, 436, 568, 1396, 1557
BtsIMutI CAGTG 1 cut(s) 1323
Cac8I GCNNGC 4 cut(s) 65, 436, 1283, 1410
CfoI GCGC 3 cut(s) 1039, 1281, 1428
Cfr10I RCCGGY 1 cut(s) 434
Cfr13I GGNCC 3 cut(s) 979, 1204, 1322
Csp6I GTAC 1 cut(s) 161
CviAII CATG 9 cut(s) 73, 157, 490, 607, 670, 983, 997, 1124, 1306
CviQI GTAC 1 cut(s) 161
DdeI CTNAG 6 cut(s) 255, 905, 1072, 1213, 1275, 1286
DpnI GATC 5 cut(s) 1049, 1178, 1304, 1332, 1601
DpnII GATC 5 cut(s) 1047, 1176, 1302, 1330, 1599
Eam1104I CTCTTC 4 cut(s) 44, 798, 1129, 1538
EarI CTCTTC 4 cut(s) 44, 798, 1129, 1538
Eco130I CCWWGG 2 cut(s) 850, 996
Eco47I GGWCC 1 cut(s) 1204
Eco47III AGCGCT 2 cut(s) 1038, 1280
Eco57I CTGAAG 2 cut(s) 380, 1562
Eco88I CYCGRG 1 cut(s) 1532
EcoNI CCTNNNNNAGG 1 cut(s) 395
EcoO109I RGGNCCY 2 cut(s) 1204, 1322
EcoRI GAATTC 1 cut(s) 770
EcoRII CCWGG 2 cut(s) 975, 1413
EcoT14I CCWWGG 2 cut(s) 850, 996
ErhI CCWWGG 2 cut(s) 850, 996
FaeI CATG 9 cut(s) 76, 160, 493, 610, 673, 986, 1000, 1127, 1309
FatI CATG 9 cut(s) 72, 156, 489, 606, 669, 982, 996, 1123, 1305
FauNDI CATATG 1 cut(s) 597
FbaI TGATCA 4 cut(s) 1047, 1176, 1330, 1599
Fnu4HI GCNGC 8 cut(s) 6, 103, 142, 237, 459, 1157, 1355, 1538
FokI GGATG 7 cut(s) 316, 367, 439, 443, 575, 1403, 1544
Fsp4HI GCNGC 8 cut(s) 6, 103, 142, 237, 459, 1157, 1355, 1538
FspBI CTAG 3 cut(s) 482, 797, 1637
FspI TGCGCA 1 cut(s) 1427
GlaI GCGC 3 cut(s) 1038, 1280, 1427
GluI GCNGC 8 cut(s) 6, 103, 142, 237, 459, 1157, 1355, 1538
GsaI CCCAGC 1 cut(s) 1212
GsuI CTGGAG 3 cut(s) 178, 1181, 1436
HaeII RGCGCY 2 cut(s) 1040, 1282
HaeIII GGCC 6 cut(s) 401, 849, 980, 1070, 1149, 1324
HapII CCGG 1 cut(s) 435
HhaI GCGC 3 cut(s) 1039, 1281, 1428
Hin1II CATG 9 cut(s) 76, 160, 493, 610, 673, 986, 1000, 1127, 1309
Hin6I GCGC 3 cut(s) 1037, 1279, 1426
HinP1I GCGC 3 cut(s) 1037, 1279, 1426
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 6 cut(s) 581, 800, 1454, 1495, 1523, 1708
HpaII CCGG 1 cut(s) 435
HphI GGTGA 2 cut(s) 730, 1594
Hpy188I TCNGA 4 cut(s) 258, 749, 1173, 1383
Hpy188III TCNNGA 6 cut(s) 127, 195, 578, 797, 1433, 1478
Hpy99I CGWCG 1 cut(s) 1655
HpyAV CCTTC 8 cut(s) 23, 40, 412, 866, 1139, 1469, 1507, 1561
HpyCH4III ACNGT 4 cut(s) 782, 1254, 1318, 1484
HpyCH4IV ACGT 3 cut(s) 1052, 1343, 1653
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 69, 444, 1013, 1418
HpyF3I CTNAG 6 cut(s) 255, 905, 1072, 1213, 1275, 1286
HpySE526I ACGT 3 cut(s) 1052, 1343, 1653
Hsp92II CATG 9 cut(s) 76, 160, 493, 610, 673, 986, 1000, 1127, 1309
HspAI GCGC 3 cut(s) 1037, 1279, 1426
KpnI GGTACC 1 cut(s) 164
KroI GCCGGC 1 cut(s) 434
KroNI GCCGGC 1 cut(s) 436
Ksp22I TGATCA 4 cut(s) 1047, 1176, 1330, 1599
Kzo9I GATC 5 cut(s) 1047, 1176, 1302, 1330, 1599
LguI GCTCTTC 1 cut(s) 44
LmnI GCTCC 2 cut(s) 603, 977
Lsp1109I GCAGC 8 cut(s) 17, 114, 153, 248, 445, 1143, 1341, 1549
LweI GCATC 5 cut(s) 66, 76, 370, 421, 1566
MaeI CTAG 3 cut(s) 482, 797, 1637
MaeII ACGT 3 cut(s) 1052, 1343, 1653
MaeIII GTNAC 4 cut(s) 665, 1116, 1366, 1527
MalI GATC 5 cut(s) 1049, 1178, 1304, 1332, 1601
MboI GATC 5 cut(s) 1047, 1176, 1302, 1330, 1599
MhlI GDGCHC 1 cut(s) 974
MmeI TCCRAC 2 cut(s) 937, 1111
MroNI GCCGGC 1 cut(s) 434
MroXI GAANNNNTTC 3 cut(s) 573, 754, 1453
MseI TTAA 5 cut(s) 276, 1230, 1508, 1698, 1717
MspA1I CMGCKG 1 cut(s) 1540
MspI CCGG 1 cut(s) 435
MspR9I CCNGG 2 cut(s) 977, 1415
Mva1269I GAATGC 2 cut(s) 1246, 1428
MvaI CCWGG 2 cut(s) 977, 1415
MwoI GCNNNNNNNGC 5 cut(s) 60, 69, 444, 1013, 1418
NaeI GCCGGC 1 cut(s) 436
NcoI CCATGG 1 cut(s) 996
NdeI CATATG 1 cut(s) 597
NdeII GATC 5 cut(s) 1047, 1176, 1302, 1330, 1599
NgoMIV GCCGGC 1 cut(s) 434
NlaIII CATG 9 cut(s) 76, 160, 493, 610, 673, 986, 1000, 1127, 1309
NlaIV GGNNCC 5 cut(s) 162, 520, 1206, 1323, 1579
NmeAIII GCCGAG 2 cut(s) 1175, 1606
NsbI TGCGCA 1 cut(s) 1427
NspI RCATGY 1 cut(s) 610
PaeR7I CTCGAG 1 cut(s) 1532
PciSI GCTCTTC 1 cut(s) 44
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 2 cut(s) 1246, 1428
PdiI GCCGGC 1 cut(s) 436
PdmI GAANNNNTTC 3 cut(s) 573, 754, 1453
PfeI GAWTC 6 cut(s) 581, 800, 1454, 1495, 1523, 1708
PkrI GCNGC 8 cut(s) 7, 104, 143, 238, 460, 1158, 1356, 1539
PpuMI RGGWCCY 1 cut(s) 1204
PshBI ATTAAT 1 cut(s) 1230
Psp5II RGGWCCY 1 cut(s) 1204
Psp6I CCWGG 2 cut(s) 975, 1413
PspFI CCCAGC 1 cut(s) 1208
PspGI CCWGG 2 cut(s) 975, 1413
PspN4I GGNNCC 5 cut(s) 162, 520, 1206, 1323, 1579
PspPI GGNCC 3 cut(s) 979, 1204, 1322
PspPPI RGGWCCY 1 cut(s) 1204
PspXI VCTCGAGB 1 cut(s) 1532
PvuII CAGCTG 1 cut(s) 1540
RsaI GTAC 1 cut(s) 162
RsaNI GTAC 1 cut(s) 161
SapI GCTCTTC 1 cut(s) 44
SaqAI TTAA 5 cut(s) 276, 1230, 1508, 1698, 1717
SatI GCNGC 8 cut(s) 6, 103, 142, 237, 459, 1157, 1355, 1538
Sau3AI GATC 5 cut(s) 1047, 1176, 1302, 1330, 1599
Sau96I GGNCC 3 cut(s) 979, 1204, 1322
ScrFI CCNGG 2 cut(s) 977, 1415
SduI GDGCHC 1 cut(s) 974
SfaNI GCATC 5 cut(s) 66, 76, 370, 421, 1566
Sfr274I CTCGAG 1 cut(s) 1532
SinI GGWCC 1 cut(s) 1204
SlaI CTCGAG 1 cut(s) 1532
SmlI CTYRAG 1 cut(s) 1532
SmoI CTYRAG 1 cut(s) 1532
SsiI CCGC 3 cut(s) 572, 1268, 1469
SspMI CTAG 3 cut(s) 482, 797, 1637
StyD4I CCNGG 2 cut(s) 975, 1413
StyI CCWWGG 2 cut(s) 850, 996
TaaI ACNGT 4 cut(s) 782, 1254, 1318, 1484
TaiI ACGT 3 cut(s) 1055, 1346, 1656
TaqI TCGA 4 cut(s) 1041, 1493, 1533, 1687
TfiI GAWTC 6 cut(s) 581, 800, 1454, 1495, 1523, 1708
Tru1I TTAA 5 cut(s) 276, 1230, 1508, 1698, 1717
Tru9I TTAA 5 cut(s) 276, 1230, 1508, 1698, 1717
TscAI CASTG 1 cut(s) 1323
TseI GCWGC 8 cut(s) 5, 102, 141, 236, 458, 1156, 1354, 1537
TspDTI ATGAA 9 cut(s) 145, 320, 357, 423, 555, 582, 1026, 1112, 1407
TspGWI ACGGA 1 cut(s) 439
TspRI CASTG 1 cut(s) 1323
VpaK11BI GGWCC 1 cut(s) 1204
VspI ATTAAT 1 cut(s) 1230
XagI CCTNNNNNAGG 1 cut(s) 395
XapI RAATTY 3 cut(s) 509, 770, 1694
XbaI TCTAGA 1 cut(s) 796
XceI RCATGY 1 cut(s) 610
XhoI CTCGAG 1 cut(s) 1532
XmnI GAANNNNTTC 3 cut(s) 573, 754, 1453
XspI CTAG 3 cut(s) 482, 797, 1637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.