MD01G1223700.v1.1

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009930
Physical Location & Seq
Reverse (-)
31526724 .. 31526957
234 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1223700.v1.1.491

Sequence Viewer

Length: 234 bp
ATGAGTCTGCCGCCATCACGCGGCAAGAAATTGTGGTGCTTGCCCAGGACGGAGGCATTGCAGAGGAATATTGATTATGTTTGTTTCCTGGGGTTGGATTGTGGGCCAATCAAACAAGGTGGACCGTGCTTTGTGGCCAACACAGTTAGAGCCCATGCAAAGTATGCCATGAATGTCTACTTTCAAGCAAGAATAATGTTTAGCTATTCAAGTTTATATCAGAAAGTTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17 X8

Protein Analysis

78

Amino Acids

8.76

Weight (kDa)

9.59

Isoelectric Point (pI)

47.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 16 - 64 7.9e-11 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 177
AccII CGCG 1 cut(s) 21
AciI CCGC 2 cut(s) 11, 21
AcoI YGGCCR 1 cut(s) 135
AfiI CCNNNNNNNGG 2 cut(s) 20, 94
AgsI TTSAA 2 cut(s) 185, 210
AjnI CCWGG 2 cut(s) 44, 87
AluBI AGCT 1 cut(s) 204
AluI AGCT 1 cut(s) 204
AoxI GGCC 2 cut(s) 104, 135
AspS9I GGNCC 2 cut(s) 104, 122
AvaII GGWCC 1 cut(s) 122
BalI TGGCCA 1 cut(s) 137
BanII GRGCYC 1 cut(s) 154
BccI CCATC 1 cut(s) 22
BciT130I CCWGG 2 cut(s) 46, 89
BisI GCNGC 2 cut(s) 11, 22
BlsI GCNGC 2 cut(s) 12, 23
Bme1390I CCNGG 2 cut(s) 46, 89
Bme18I GGWCC 1 cut(s) 122
BmgT120I GGNCC 2 cut(s) 104, 122
BmrFI CCNGG 2 cut(s) 46, 89
BsaJI CCNNGG 2 cut(s) 44, 88
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 94
Bse3DI GCAATG 1 cut(s) 56
BseBI CCWGG 2 cut(s) 46, 89
BseDI CCNNGG 2 cut(s) 44, 88
BseLI CCNNNNNNNGG 2 cut(s) 20, 94
BseMI GCAATG 1 cut(s) 56
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 2 cut(s) 106, 137
BslI CCNNNNNNNGG 2 cut(s) 20, 94
BsnI GGCC 2 cut(s) 106, 137
Bsp1286I GDGCHC 1 cut(s) 154
BspACI CCGC 2 cut(s) 11, 21
BspANI GGCC 2 cut(s) 106, 137
BspFNI CGCG 1 cut(s) 21
BsrDI GCAATG 1 cut(s) 56
BssECI CCNNGG 2 cut(s) 44, 88
Bst2UI CCWGG 2 cut(s) 46, 89
Bst4CI ACNGT 2 cut(s) 126, 145
BstAPI GCANNNNNTGC 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 41
BstFNI CGCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNI CCWGG 2 cut(s) 46, 89
BstSCI CCNGG 2 cut(s) 44, 87
BstUI CGCG 1 cut(s) 21
BsuRI GGCC 2 cut(s) 106, 137
Cac8I GCNNGC 1 cut(s) 41
Cfr13I GGNCC 2 cut(s) 104, 122
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 2 cut(s) 155, 169
CviJI RGCY 4 cut(s) 106, 137, 152, 204
CviKI_1 RGCY 4 cut(s) 106, 137, 152, 204
EaeI YGGCCR 1 cut(s) 135
Eco24I GRGCYC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 122
EcoRII CCWGG 2 cut(s) 44, 87
EcoT38I GRGCYC 1 cut(s) 154
FaeI CATG 2 cut(s) 158, 172
FaiI YATR 5 cut(s) 78, 156, 165, 170, 217
FatI CATG 2 cut(s) 154, 168
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 2 cut(s) 11, 22
FriOI GRGCYC 1 cut(s) 154
Fsp4HI GCNGC 2 cut(s) 11, 22
GluI GCNGC 2 cut(s) 11, 22
HaeIII GGCC 2 cut(s) 106, 137
Hin1II CATG 2 cut(s) 158, 172
HinfI GANTC 1 cut(s) 4
Hpy166II GTNNAC 2 cut(s) 122, 178
Hpy188I TCNGA 1 cut(s) 222
Hpy8I GTNNAC 2 cut(s) 122, 178
HpyCH4III ACNGT 2 cut(s) 126, 145
HpyCH4V TGCA 2 cut(s) 61, 158
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
Hsp92II CATG 2 cut(s) 158, 172
LpnPI CCDG 4 cut(s) 31, 58, 74, 101
MhlI GDGCHC 1 cut(s) 154
MlsI TGGCCA 1 cut(s) 137
MluCI AATT 1 cut(s) 29
MluNI TGGCCA 1 cut(s) 137
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 2 cut(s) 46, 57
Mox20I TGGCCA 1 cut(s) 137
MscI TGGCCA 1 cut(s) 137
Msp20I TGGCCA 1 cut(s) 137
MspR9I CCNGG 2 cut(s) 46, 89
MvaI CCWGG 2 cut(s) 46, 89
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 1 cut(s) 164
NlaIII CATG 2 cut(s) 158, 172
PkrI GCNGC 2 cut(s) 12, 23
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
Psp6I CCWGG 2 cut(s) 44, 87
PspGI CCWGG 2 cut(s) 44, 87
PspPI GGNCC 2 cut(s) 104, 122
SatI GCNGC 2 cut(s) 11, 22
Sau96I GGNCC 2 cut(s) 104, 122
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 2 cut(s) 46, 89
SduI GDGCHC 1 cut(s) 154
SetI ASST 2 cut(s) 121, 206
SinI GGWCC 1 cut(s) 122
Sse9I AATT 1 cut(s) 29
SsiI CCGC 2 cut(s) 11, 21
SspI AATATT 1 cut(s) 70
StyD4I CCNGG 2 cut(s) 44, 87
TaaI ACNGT 2 cut(s) 126, 145
TasI AATT 1 cut(s) 29
TauI GCSGC 2 cut(s) 13, 24
TspDTI ATGAA 1 cut(s) 185
TspGWI ACGGA 1 cut(s) 65
VpaK11BI GGWCC 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.