Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0006352

Physical Location & Seq

Genomic Coordinates Forward (+)

32583217 .. 32592850

Gene Structure 9634 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1239700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2853 bp

ATGGATGAGCTCAGCCCTAGTCCTCAATCCAAAGACTCTGCCGCTTCTTCGTGGCGTCCCGGCCAGCTCGCATTTAGTCCTTATTCTACGCAGAGTGAAGCTGCCAACAACAAATCCCAATGTCTGCGTGTCCTTGTCAGGAGACCTTTTGTGGCAAGGCTAACCAAGGACATAGTTGAAACATATCAAATATGCAATCCACAGTTCAAGTATTCAGAAGAATTGAATCCAAAGAGATATTTGACCACCCCGTCAAGTGGAGTACTCAACGATGGCTATGATAATGTCAACTCAGATCTTATTTTGACTGTAAACTTTGTCTTGGTCAATCTAGACACACAGCGAAGATACATTGTCAAAGATGTTCTTGGCCATGGGACTTTTGGCCAGGTTGCTAAATGCTTGGTTCCACAGACTAACAGCTTCGTAGCTGTGAAGATCATCAAAAATCAACCAGCATACTATCAACAAGCCCTGGTTGAAGTATCCATTTTGACAACGTTAAATAAGAAATATGATCCTGAGGATAAGCATCACATTGTGCGTATATATGATTACTTTGTGTGTCATCGTCATTTGTGCATTTGCTTTGAACTGCTTGATACGAATCTGTATGAGCTTATCAAGATAAATCATTTCAGGGGACTATCCCTGACCATTGTTCAATTGTTTTCCAAACAAATTCTACATGGGTTGGCTCTGTTAAAAGATGCTGGGATAATTCACTGTGATCTGAAGCCGGAAAACATTCTTTTGTGTACAAGTGTTAAGCCAGCAGAAATTAAAATTATTGACTTTGGATCAGCCTGCATGGAAGATCGAACTGTTTACTCCTATATCCAGAGTCGTTACTATAGATCTCCTGAAGTTCTCCTTGGGTATCAATATACTACTTCTATCGATATGTGGTCCTTTGGATGTATAGTCGCCGAATTGTTTCTGGGGTTGCCATTGTTCCCAGGAGCTTCAGAATTTGACCTCCTAAGGCGTATGATTGAAATACTTGGAGGGCAGCCCCCTGATTATGTACTTAAGGAGGCAAAAAACACAAGCAAGTTCTTTAAGTGCATTCGGAGTGTGCATAATATGGAGAACGGGGAACTTTCTGCAAATGGAAAAAGTGCTTATCAAGCATTAACAGAAGCAGAATATGAAGCTAGAGAATTGAAAAGGCCATCAATTGGGAAAGAGTATTTTAATCATATGAACCTTGAAGAAATTGTTACAAGCTATCCGTATAGGAAGAACTTGCCTAAGGAAGACATGGTTAAAGAAAATCAAATAAGACTAGCTTTGGTTGATTTTTTGAAGGGACTGGTTGAGTTTGATCCTGCAAAACGCTGGTCACCTGTTCAGGCTTCAAAACACCCTTTTGTTACGGGGGAGCCTTTTACATGCCCTTATAAACCTCCCTCGGAGACCCCTCGCATGCCCGTTTCTCAAAACATAAGGGTGGACCATCATCCTGGTGGTGGGCATTGGTTTGCGGCTGGTTTATCTCCGAATATTCCAGGCAGAAATAGGGTCTCCATTCATAGCAGCCCACATTTTCAGGTGGTGCCATATGCACATGCTAATAGCTATGGCAGTGTAGGAAGCCATGGTAGCTATAATGATGGTACTGGATTGGGAAGCAGCTATGGAAGTTATGGAGATACTAGTAATATATTTGCTTATTATTCCCCAGTTGGCCCTTCTGGGATGAACATGCATGCACAAGGTAATTTATCAATGCTTGGAAGTAGTCCGGATGCAAGACGGAGAATTGTTCAGTATTCACATGGAAATGGACTTGGTATGAGTCCTTCAGCAGGAAGCTTTGCTCCACTACCTCTAGGCACTAGTCCTTCGCAATTTACTCCGCCAAGCTCCTACGGTCAAGTTTCTGCTGGCTCTCCTGGACATTATGGTCCAACTTCTCCAGCAAGGGGCAATTGTCATGGATCACCTTTAGGAAAGATGGCTGCTGTTAGCCAGTTTAATAGACGAAAAAGTTGGGGATATCCTGGAGGTTCTCAAACTCAAGAAAGTTCATCAACACATTGGCAAGGGCAAGCTACTGATGGCACCAGTTCTAGCCAAGCTGAGGGAAACTCTCAAATGCTAGGTAGTTCACCATCACATCTGCATTTAAGCTCAAATGCTGGAAGCTGGAAGCAGCCAAGAGGAGGCAGTGGTATTTCTGCTGGGTACTTAGCCCAGAACATGCCAGCTTCCATCACAGTTGGCTCCCATATACAATTCACAAAGACCACGGGAGTGTCTCATGAAAAGGCTGAGGCTATCCAGTCATTGCCTGATCCTGGGGATTGGGATCCCAACTATAGTGATGAACTTCTTCTGCAAGAAGATGGTTCGGATGTAAGCTGCTTTACTGCTGAGTTCAGCCAAGGCATGCATTTTAGTTCTGCAGAAACATTGGTTGGGTCCGGAAGATTTAACCATGCGCCAAATTCAAGCACGGGCCCATCCTTTCAAAGACAAAATGGACCCATTCAATCATATCCACATTCCGAGGTGGGTAGCCCTCCTCCAACTACTGAACCACTTGCAGGATACATGCGTTTGTCAAAACCTTCTCATTTCATGCCTCACATATCACAAAATTCTCCAAGCCGCTTGGGACAGCAATATCAGCGATCAAATCATGGACGACCCACCAATTCTCGTGGTAATGATTGGAATCATATGAAGATGCAGCCCCCTTCTCCCAATTTTAACTCTGGAGGCCCACATTCTCCAGGAAATAGCTCATTCAGCAATGGCATGTCATGGGGGCATAGAGCCAGTCATCCCATCACTAGCATTCCACCTGCATCTCGAGGAAGAAAGGACTATGGAAGGATTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006950 GO:0006970 GO:0006972 GO:0006979 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009267 GO:0009605 GO:0009628 GO:0009892 GO:0009893 GO:0009987 GO:0009991 GO:0010033 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0019932 GO:0019933 GO:0019935 GO:0023052 GO:0030587 GO:0031152 GO:0031667 GO:0031668 GO:0031669 GO:0032502 GO:0033554 GO:0035556 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0042594 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044764 GO:0045472 GO:0046677 GO:0048518 GO:0048519 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051409 GO:0051703 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0071496 GO:0071704 GO:0090702 GO:0097327 GO:0098630 GO:0098743 GO:0099120 GO:0140096 GO:1901564 GO:1901654 GO:1901700 GO:1904643

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

951

Amino Acids

104.4

Weight (kDa)

8.6

Isoelectric Point (pI)

54.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	117 - 459	3.8e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	120 - 331	3.9e-21	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006352)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35980 AT5G35980 AT5G35980
fragaria_vesca	FvH4_7g34270 FvH4_7g34270 FvH4_7g34270 FvH4_7g34270
malus_domestica	MD01G1239700.v1.1 MD07G1311600.v1.1
prunus_persica	Prupe.2G329600_v2.0.a1 Prupe.2G329600_v2.0.a1
pyrus_communis	pycom07g28380
rosa_chinensis	RchiOBHm_Chr1g0384621
rosa_laevigata	RLG00000026026
rosa_multiflora	Rmu_co8382885.1_g000001 Rmu_sc0012136.1_g000028
rosa_roxburghii	Rroxscaffold_1G00045450
rosa_rugosa	Rorug01G0450200
rosa_samantha	Rh1AG474700 Rh1BG432800 Rh1CG446000 Rh1DG465400 Rh2AG390000 Rh3CG154800 Rh3DG155900 Rh4CG245100
rosa_wichuraiana	Rw1G039790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1404
AarI	CACCTGC	1 cut(s)	2821
AasI	GACNNNNNNGTC	2 cut(s)	250, 1908
Acc36I	ACCTGC	1 cut(s)	2821
AccB1I	GGYRCC	2 cut(s)	1558, 2066
AccB7I	CCANNNNNTGG	1 cut(s)	1181
AccIII	TCCGGA	2 cut(s)	1747, 2429
AciI	CCGC	4 cut(s)	42, 1487, 1862, 2617
AclI	AACGTT	1 cut(s)	500
AclWI	GGATC	7 cut(s)	512, 808, 1322, 1951, 2294, 2309, 2322
AcoI	YGGCCR	3 cut(s)	61, 370, 385
AcsI	RAATTY	4 cut(s)	681, 971, 2452, 2605
AcuI	CTGAAG	4 cut(s)	755, 885, 951, 1791
AcyI	GRCGYC	1 cut(s)	55
AdeI	CACNNNGTG	1 cut(s)	541
AfaI	GTAC	5 cut(s)	264, 760, 1029, 1621, 2192
AfiI	CCNNNNNNNGG	9 cut(s)	257, 1181, 1472, 1811, 1928, 2086, 2168, 2303, 2552
AflII	CTTAAG	1 cut(s)	1031
AhlI	ACTAGT	2 cut(s)	1658, 1841
AjnI	CCWGG	9 cut(s)	387, 474, 958, 1465, 1510, 1897, 2005, 2302, 2740
AjuI	GAANNNNNNNTTGG	4 cut(s)	858, 890, 2406, 2438
AleI	CACNNNNGTG	1 cut(s)	2258
Alw21I	GWGCWC	1 cut(s)	12
Alw26I	GTCTC	4 cut(s)	136, 1412, 1531, 2268
AlwI	GGATC	7 cut(s)	512, 808, 1322, 1951, 2294, 2309, 2322
Ama87I	CYCGRG	1 cut(s)	2820
Aor13HI	TCCGGA	2 cut(s)	1747, 2429
AoxI	GGCC	7 cut(s)	61, 370, 385, 1172, 1690, 2464, 2728
ApaI	GGGCCC	1 cut(s)	2468
ApeKI	GCWGC	8 cut(s)	101, 1012, 1539, 1635, 1964, 2158, 2367, 2698
ApoI	RAATTY	4 cut(s)	681, 971, 2452, 2605
ArsI	GACNNNNNNTTYG	2 cut(s)	1328, 1360
Asp700I	GAANNNNTTC	4 cut(s)	747, 936, 2337, 2845
AspLEI	GCGC	1 cut(s)	2449
AspS9I	GGNCC	9 cut(s)	909, 1456, 1691, 1910, 2427, 2464, 2465, 2489, 2729
AsuC2I	CCSGG	1 cut(s)	60
AsuHPI	GGTGA	3 cut(s)	1338, 1938, 2106
AvaI	CYCGRG	1 cut(s)	2820
AvaII	GGWCC	5 cut(s)	909, 1456, 1910, 2427, 2489
AxyI	CCTNAGG	3 cut(s)	522, 983, 1254
BaeGI	GKGCMC	1 cut(s)	2468
BaeI	ACNNNNGTAYC	2 cut(s)	2548, 2581
BalI	TGGCCA	2 cut(s)	372, 387
BamHI	GGATCC	1 cut(s)	2314
BanI	GGYRCC	2 cut(s)	1558, 2066
BanII	GRGCYC	2 cut(s)	12, 2468
BarI	GAAGNNNNNNTAC	2 cut(s)	1789, 1821
BauI	CACGAG	1 cut(s)	2667
BbsI	GAAGAC	1 cut(s)	1266
Bbv12I	GWGCWC	1 cut(s)	12
BbvCI	CCTCAGC	2 cut(s)	2085, 2277
BbvI	GCAGC	8 cut(s)	88, 1024, 1551, 1647, 1951, 2170, 2354, 2710
BciT130I	CCWGG	9 cut(s)	389, 476, 960, 1467, 1512, 1899, 2007, 2304, 2742
BciVI	GTATCC	2 cut(s)	496, 2549
BcnI	CCSGG	1 cut(s)	60
BcoDI	GTCTC	4 cut(s)	136, 1412, 1531, 2268
BcuI	ACTAGT	2 cut(s)	1658, 1841
BfmI	CTRYAG	3 cut(s)	853, 2323, 2409
BfrI	CTTAAG	1 cut(s)	1031
BfuAI	ACCTGC	1 cut(s)	2821
BfuI	GTATCC	2 cut(s)	496, 2549
BglII	AGATCT	2 cut(s)	295, 857
BlpI	GCTNAGC	1 cut(s)	11
BmcAI	AGTACT	1 cut(s)	264
Bme18I	GGWCC	5 cut(s)	909, 1456, 1910, 2427, 2489
BmeT110I	CYCGRG	1 cut(s)	2820
BmgT120I	GGNCC	9 cut(s)	909, 1456, 1691, 1910, 2427, 2464, 2465, 2489, 2729
BmiI	GGNNCC	9 cut(s)	408, 1386, 1560, 2068, 2230, 2316, 2428, 2466, 2491
BmrI	ACTGGG	1 cut(s)	1679
BmsI	GCATC	5 cut(s)	541, 700, 1741, 2685, 2825
BmuI	ACTGGG	1 cut(s)	1679
BpiI	GAAGAC	1 cut(s)	1266
BplI	GAGNNNNNCTC	2 cut(s)	2078, 2110
BpmI	CTGGAG	4 cut(s)	1905, 2028, 2724, 2745
Bpu10I	CCTNAGC	2 cut(s)	2085, 2277
Bpu1102I	GCTNAGC	1 cut(s)	11
BpuEI	CTTGAG	1 cut(s)	2007
BpuMI	CCSGG	1 cut(s)	60
Bsa29I	ATCGAT	1 cut(s)	900
BsaBI	GATNNNNATC	4 cut(s)	531, 606, 2313, 2682
BsaHI	GRCGYC	1 cut(s)	55
BsaI	GGTCTC	3 cut(s)	136, 1412, 1531
BsaWI	WCCGGW	2 cut(s)	1747, 2429
Bsc4I	CCNNNNNNNGG	9 cut(s)	257, 1181, 1472, 1811, 1928, 2086, 2168, 2303, 2552
Bse1I	ACTGG	7 cut(s)	1320, 1627, 1685, 1975, 2070, 2287, 2787
Bse21I	CCTNAGG	3 cut(s)	522, 983, 1254
Bse3DI	GCAATG	2 cut(s)	2291, 2767
Bse8I	GATNNNNATC	4 cut(s)	531, 606, 2313, 2682
BseAI	TCCGGA	2 cut(s)	1747, 2429
BseBI	CCWGG	9 cut(s)	389, 476, 960, 1467, 1512, 1899, 2007, 2304, 2742
BseCI	ATCGAT	1 cut(s)	900
BseGI	GGATG	8 cut(s)	10, 923, 1462, 1707, 1756, 2365, 2468, 2791
BseJI	GATNNNNATC	4 cut(s)	531, 606, 2313, 2682
BseLI	CCNNNNNNNGG	9 cut(s)	257, 1181, 1472, 1811, 1928, 2086, 2168, 2303, 2552
BseMI	GCAATG	2 cut(s)	2291, 2767
BseMII	CTCAG	6 cut(s)	25, 306, 513, 2076, 2268, 2370
BseNI	ACTGG	7 cut(s)	1320, 1627, 1685, 1975, 2070, 2287, 2787
BseRI	GAGGAG	2 cut(s)	2181, 2520
BseSI	GKGCMC	1 cut(s)	2468
BseXI	GCAGC	8 cut(s)	88, 1024, 1551, 1647, 1951, 2170, 2354, 2710
BseYI	CCCAGC	2 cut(s)	713, 2186
BshFI	GGCC	7 cut(s)	63, 372, 387, 1174, 1692, 2466, 2730
BshNI	GGYRCC	2 cut(s)	1558, 2066
BshVI	ATCGAT	1 cut(s)	900
BsiHKAI	GWGCWC	1 cut(s)	12
BsiHKCI	CYCGRG	1 cut(s)	2820
BsiSI	CCGG	4 cut(s)	60, 740, 1748, 2430
BslFI	GGGAC	5 cut(s)	42, 391, 657, 1326, 2637
BslI	CCNNNNNNNGG	9 cut(s)	257, 1181, 1472, 1811, 1928, 2086, 2168, 2303, 2552
BsmAI	GTCTC	4 cut(s)	136, 1412, 1531, 2268
BsmFI	GGGAC	5 cut(s)	42, 391, 657, 1326, 2637
BsmI	GAATGC	2 cut(s)	1068, 2805
BsnI	GGCC	7 cut(s)	63, 372, 387, 1174, 1692, 2466, 2730
Bso31I	GGTCTC	3 cut(s)	136, 1412, 1531
BsoBI	CYCGRG	1 cut(s)	2820
Bsp120I	GGGCCC	1 cut(s)	2464
Bsp1286I	GDGCHC	2 cut(s)	12, 2468
Bsp13I	TCCGGA	2 cut(s)	1747, 2429
Bsp1407I	TGTACA	1 cut(s)	758
Bsp1720I	GCTNAGC	1 cut(s)	11
Bsp19I	CCATGG	2 cut(s)	373, 1600
BspACI	CCGC	4 cut(s)	42, 1487, 1862, 2617
BspANI	GGCC	7 cut(s)	63, 372, 387, 1174, 1692, 2466, 2730
BspCNI	CTCAG	6 cut(s)	24, 305, 514, 2077, 2269, 2371
BspDI	ATCGAT	1 cut(s)	900
BspEI	TCCGGA	2 cut(s)	1747, 2429
BspHI	TCATGA	1 cut(s)	2266
BspLI	GGNNCC	9 cut(s)	408, 1386, 1560, 2068, 2230, 2316, 2428, 2466, 2491
BspMAI	CTGCAG	1 cut(s)	2413
BspMI	ACCTGC	1 cut(s)	2821
BspPI	GGATC	7 cut(s)	512, 808, 1322, 1951, 2294, 2309, 2322
BspT107I	GGYRCC	2 cut(s)	1558, 2066
BspTI	CTTAAG	1 cut(s)	1031
BspTNI	GGTCTC	3 cut(s)	136, 1412, 1531
BsrDI	GCAATG	2 cut(s)	2291, 2767
BsrGI	TGTACA	1 cut(s)	758
BsrI	ACTGG	7 cut(s)	1320, 1627, 1685, 1975, 2070, 2287, 2787
BssNI	GRCGYC	1 cut(s)	55
BssSI	CACGAG	1 cut(s)	2667
BssT1I	CCWWGG	5 cut(s)	165, 373, 874, 1600, 2389
Bst2BI	CACGAG	1 cut(s)	2667
Bst2UI	CCWGG	9 cut(s)	389, 476, 960, 1467, 1512, 1899, 2007, 2304, 2742
Bst4CI	ACNGT	6 cut(s)	204, 310, 728, 826, 1877, 2224
BstACI	GRCGYC	1 cut(s)	55
BstAFI	CTTAAG	1 cut(s)	1031
BstAUI	TGTACA	1 cut(s)	758
BstDEI	CTNAG	9 cut(s)	11, 292, 522, 983, 1254, 2085, 2194, 2277, 2379
BstDSI	CCRYGG	3 cut(s)	373, 1600, 2253
BstEII	GGTNACC	1 cut(s)	1344
BstENI	CCTNNNNNAGG	1 cut(s)	1809
BstF5I	GGATG	8 cut(s)	10, 923, 1462, 1707, 1756, 2365, 2468, 2791
BstHHI	GCGC	1 cut(s)	2449
BstMAI	GTCTC	4 cut(s)	136, 1412, 1531, 2268
BstMWI	GCNNNNNNNGC	4 cut(s)	1130, 1605, 2635, 2757
BstNI	CCWGG	9 cut(s)	389, 476, 960, 1467, 1512, 1899, 2007, 2304, 2742
BstNSI	RCATGY	9 cut(s)	1398, 1432, 1574, 1711, 1715, 2209, 2398, 2563, 2770
BstPI	GGTNACC	1 cut(s)	1344
BstSFI	CTRYAG	3 cut(s)	853, 2323, 2409
BstSLI	GKGCMC	1 cut(s)	2468
BstV1I	GCAGC	8 cut(s)	88, 1024, 1551, 1647, 1951, 2170, 2354, 2710
BstV2I	GAAGAC	1 cut(s)	1266
BstX2I	RGATCY	3 cut(s)	295, 857, 2314
BstXI	CCANNNNNNTGG	1 cut(s)	1466
BstYI	RGATCY	3 cut(s)	295, 857, 2314
Bsu15I	ATCGAT	1 cut(s)	900
Bsu36I	CCTNAGG	3 cut(s)	522, 983, 1254
BsuI	GTATCC	2 cut(s)	496, 2549
BsuRI	GGCC	7 cut(s)	63, 372, 387, 1174, 1692, 2466, 2730
BsuTUI	ATCGAT	1 cut(s)	900
BtgI	CCRYGG	3 cut(s)	373, 1600, 2253
BtsCI	GGATG	8 cut(s)	10, 923, 1462, 1707, 1756, 2365, 2468, 2791
BtsI	GCAGTG	2 cut(s)	1594, 2179
BtsIMutI	CAGTG	3 cut(s)	724, 1594, 2179
BveI	ACCTGC	1 cut(s)	2821
CciI	TCATGA	1 cut(s)	2266
CfoI	GCGC	1 cut(s)	2449
Cfr13I	GGNCC	9 cut(s)	909, 1456, 1691, 1910, 2427, 2464, 2465, 2489, 2729
ClaI	ATCGAT	1 cut(s)	900
CseI	GACGC	1 cut(s)	44
Csp6I	GTAC	5 cut(s)	263, 759, 1028, 1620, 2191
CspCI	CAANNNNNGTGG	2 cut(s)	2650, 2685
CviQI	GTAC	5 cut(s)	263, 759, 1028, 1620, 2191
DdeI	CTNAG	9 cut(s)	11, 292, 522, 983, 1254, 2085, 2194, 2277, 2379
DraIII	CACNNNGTG	1 cut(s)	541
DrdI	GACNNNNNNGTC	2 cut(s)	250, 1908
DseDI	GACNNNNNNGTC	2 cut(s)	250, 1908
EaeI	YGGCCR	3 cut(s)	61, 370, 385
EciI	GGCGGA	1 cut(s)	1851
Ecl136II	GAGCTC	1 cut(s)	10
Eco130I	CCWWGG	5 cut(s)	165, 373, 874, 1600, 2389
Eco24I	GRGCYC	2 cut(s)	12, 2468
Eco31I	GGTCTC	3 cut(s)	136, 1412, 1531
Eco32I	GATATC	1 cut(s)	2003
Eco47I	GGWCC	5 cut(s)	909, 1456, 1910, 2427, 2489
Eco53kI	GAGCTC	1 cut(s)	10
Eco57I	CTGAAG	4 cut(s)	755, 885, 951, 1791
Eco81I	CCTNAGG	3 cut(s)	522, 983, 1254
Eco88I	CYCGRG	1 cut(s)	2820
Eco91I	GGTNACC	1 cut(s)	1344
EcoICRI	GAGCTC	1 cut(s)	10
EcoNI	CCTNNNNNAGG	1 cut(s)	1809
EcoO65I	GGTNACC	1 cut(s)	1344
EcoRII	CCWGG	9 cut(s)	387, 474, 958, 1465, 1510, 1897, 2005, 2302, 2740
EcoRV	GATATC	1 cut(s)	2003
EcoT14I	CCWWGG	5 cut(s)	165, 373, 874, 1600, 2389
EcoT22I	ATGCAT	2 cut(s)	1713, 2400
EcoT38I	GRGCYC	2 cut(s)	12, 2468
ErhI	CCWWGG	5 cut(s)	165, 373, 874, 1600, 2389
FalI	AAGNNNNNCTT	7 cut(s)	351, 383, 858, 890, 1276, 1308, 2833
FaqI	GGGAC	5 cut(s)	42, 391, 657, 1326, 2637
FauNDI	CATATG	3 cut(s)	1203, 1564, 2688
FokI	GGATG	8 cut(s)	17, 930, 1449, 1714, 1763, 2372, 2455, 2778
FriOI	GRGCYC	2 cut(s)	12, 2468
GlaI	GCGC	1 cut(s)	2448
GsaI	CCCAGC	2 cut(s)	717, 2190
GsuI	CTGGAG	4 cut(s)	1905, 2028, 2724, 2745
HaeIII	GGCC	7 cut(s)	63, 372, 387, 1174, 1692, 2466, 2730
HapII	CCGG	4 cut(s)	60, 740, 1748, 2430
HgaI	GACGC	1 cut(s)	44
HhaI	GCGC	1 cut(s)	2449
Hin1I	GRCGYC	1 cut(s)	55
Hin6I	GCGC	1 cut(s)	2447
HinP1I	GCGC	1 cut(s)	2447
HincII	GTYRAC	1 cut(s)	289
HindII	GTYRAC	1 cut(s)	289
HindIII	AAGCTT	1 cut(s)	1816
HinfI	GANTC	6 cut(s)	35, 226, 607, 844, 1801, 2683
HpaII	CCGG	4 cut(s)	60, 740, 1748, 2430
HphI	GGTGA	3 cut(s)	1338, 1938, 2106
Hpy166II	GTNNAC	6 cut(s)	289, 313, 759, 829, 1456, 2114
Hpy188I	TCNGA	9 cut(s)	217, 295, 735, 970, 1074, 1417, 1503, 2359, 2515
Hpy8I	GTNNAC	6 cut(s)	289, 313, 759, 829, 1456, 2114
HpyAV	CCTTC	7 cut(s)	1303, 1704, 1815, 1857, 2586, 2715, 2835
HpyCH4III	ACNGT	6 cut(s)	204, 310, 728, 826, 1877, 2224
HpyCH4IV	ACGT	1 cut(s)	500
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1130, 1605, 2635, 2757
HpyF3I	CTNAG	9 cut(s)	11, 292, 522, 983, 1254, 2085, 2194, 2277, 2379
HpySE526I	ACGT	1 cut(s)	500
Hsp92I	GRCGYC	1 cut(s)	55
HspAI	GCGC	1 cut(s)	2447
Kpn2I	TCCGGA	2 cut(s)	1747, 2429
LmnI	GCTCC	5 cut(s)	962, 1384, 1828, 1874, 2234
Lsp1109I	GCAGC	8 cut(s)	88, 1024, 1551, 1647, 1951, 2170, 2354, 2710
LweI	GCATC	5 cut(s)	541, 700, 1741, 2685, 2825
MaeII	ACGT	1 cut(s)	500
MaeIII	GTNAC	4 cut(s)	848, 1222, 1344, 1375
MfeI	CAATTG	3 cut(s)	665, 1179, 1933
MflI	RGATCY	3 cut(s)	295, 857, 2314
MhlI	GDGCHC	2 cut(s)	12, 2468
MlsI	TGGCCA	2 cut(s)	372, 387
MluNI	TGGCCA	2 cut(s)	372, 387
MlyI	GAGTC	3 cut(s)	29, 853, 1810
MmeI	TCCRAC	2 cut(s)	1937, 2558
Mox20I	TGGCCA	2 cut(s)	372, 387
Mph1103I	ATGCAT	2 cut(s)	1713, 2400
MroI	TCCGGA	2 cut(s)	1747, 2429
MroXI	GAANNNNTTC	4 cut(s)	747, 936, 2337, 2845
MscI	TGGCCA	2 cut(s)	372, 387
MslI	CAYNNNNRTG	4 cut(s)	1451, 1467, 1785, 2258
Msp20I	TGGCCA	2 cut(s)	372, 387
MspCI	CTTAAG	1 cut(s)	1031
MspI	CCGG	4 cut(s)	60, 740, 1748, 2430
MunI	CAATTG	3 cut(s)	665, 1179, 1933
Mva1269I	GAATGC	2 cut(s)	1068, 2805
MvaI	CCWGG	9 cut(s)	389, 476, 960, 1467, 1512, 1899, 2007, 2304, 2742
MwoI	GCNNNNNNNGC	4 cut(s)	1130, 1605, 2635, 2757
NciI	CCSGG	1 cut(s)	60
NcoI	CCATGG	2 cut(s)	373, 1600
NdeI	CATATG	3 cut(s)	1203, 1564, 2688
NlaIV	GGNNCC	9 cut(s)	408, 1386, 1560, 2068, 2230, 2316, 2428, 2466, 2491
NmuCI	GTSAC	1 cut(s)	1344
NsiI	ATGCAT	2 cut(s)	1713, 2400
NspI	RCATGY	9 cut(s)	1398, 1432, 1574, 1711, 1715, 2209, 2398, 2563, 2770
OliI	CACNNNNGTG	1 cut(s)	2258
PaeI	GCATGC	3 cut(s)	1432, 1715, 2398
PaeR7I	CTCGAG	1 cut(s)	2820
PagI	TCATGA	1 cut(s)	2266
PaqCI	CACCTGC	1 cut(s)	2821
PctI	GAATGC	2 cut(s)	1068, 2805
PdmI	GAANNNNTTC	4 cut(s)	747, 936, 2337, 2845
PfeI	GAWTC	3 cut(s)	226, 607, 2683
PflMI	CCANNNNNTGG	1 cut(s)	1181
PfoI	TCCNGGA	3 cut(s)	1897, 2005, 2740
PleI	GAGTC	3 cut(s)	29, 852, 1809
PpsI	GAGTC	3 cut(s)	29, 852, 1809
PsiI	TTATAA	1 cut(s)	1404
Psp124BI	GAGCTC	1 cut(s)	12
Psp1406I	AACGTT	1 cut(s)	500
Psp6I	CCWGG	9 cut(s)	387, 474, 958, 1465, 1510, 1897, 2005, 2302, 2740
PspEI	GGTNACC	1 cut(s)	1344
PspFI	CCCAGC	2 cut(s)	713, 2186
PspGI	CCWGG	9 cut(s)	387, 474, 958, 1465, 1510, 1897, 2005, 2302, 2740
PspN4I	GGNNCC	9 cut(s)	408, 1386, 1560, 2068, 2230, 2316, 2428, 2466, 2491
PspOMI	GGGCCC	1 cut(s)	2464
PspPI	GGNCC	9 cut(s)	909, 1456, 1691, 1910, 2427, 2464, 2465, 2489, 2729
PstI	CTGCAG	1 cut(s)	2413
PsuI	RGATCY	3 cut(s)	295, 857, 2314
RsaI	GTAC	5 cut(s)	264, 760, 1029, 1621, 2192
RsaNI	GTAC	5 cut(s)	263, 759, 1028, 1620, 2191
RseI	CAYNNNNRTG	4 cut(s)	1451, 1467, 1785, 2258
SacI	GAGCTC	1 cut(s)	12
Sau96I	GGNCC	9 cut(s)	909, 1456, 1691, 1910, 2427, 2464, 2465, 2489, 2729
ScaI	AGTACT	1 cut(s)	264
SchI	GAGTC	3 cut(s)	29, 853, 1810
SduI	GDGCHC	2 cut(s)	12, 2468
SfaNI	GCATC	5 cut(s)	541, 700, 1741, 2685, 2825
SfcI	CTRYAG	3 cut(s)	853, 2323, 2409
Sfr274I	CTCGAG	1 cut(s)	2820
SinI	GGWCC	5 cut(s)	909, 1456, 1910, 2427, 2489
SlaI	CTCGAG	1 cut(s)	2820
SmiMI	CAYNNNNRTG	4 cut(s)	1451, 1467, 1785, 2258
SmlI	CTYRAG	3 cut(s)	1031, 2022, 2820
SmoI	CTYRAG	3 cut(s)	1031, 2022, 2820
SpeI	ACTAGT	2 cut(s)	1658, 1841
SphI	GCATGC	3 cut(s)	1432, 1715, 2398
SsiI	CCGC	4 cut(s)	42, 1487, 1862, 2617
SspI	AATATT	1 cut(s)	1507
SstI	GAGCTC	1 cut(s)	12
StyI	CCWWGG	5 cut(s)	165, 373, 874, 1600, 2389
TaaI	ACNGT	6 cut(s)	204, 310, 728, 826, 1877, 2224
TaiI	ACGT	1 cut(s)	503
TaqI	TCGA	3 cut(s)	820, 900, 2821
TatI	WGTACW	3 cut(s)	262, 758, 1027
TauI	GCSGC	3 cut(s)	44, 1490, 2619
TfiI	GAWTC	3 cut(s)	226, 607, 2683
TscAI	CASTG	3 cut(s)	731, 1594, 2179
TseFI	GTSAC	1 cut(s)	1344
TseI	GCWGC	8 cut(s)	101, 1012, 1539, 1635, 1964, 2158, 2367, 2698
Tsp45I	GTSAC	1 cut(s)	1344
TspDTI	ATGAA	9 cut(s)	1167, 1220, 1523, 1718, 2022, 2283, 2346, 2575, 2705
TspGWI	ACGGA	2 cut(s)	1224, 1774
TspRI	CASTG	3 cut(s)	731, 1594, 2179
Van91I	CCANNNNNTGG	1 cut(s)	1181
Vha464I	CTTAAG	1 cut(s)	1031
VpaK11BI	GGWCC	5 cut(s)	909, 1456, 1910, 2427, 2489
XagI	CCTNNNNNAGG	1 cut(s)	1809
XapI	RAATTY	4 cut(s)	681, 971, 2452, 2605
XbaI	TCTAGA	1 cut(s)	331
XceI	RCATGY	9 cut(s)	1398, 1432, 1574, 1711, 1715, 2209, 2398, 2563, 2770
XcmI	CCANNNNNNNNNTGG	2 cut(s)	380, 1466
XhoI	CTCGAG	1 cut(s)	2820
XmnI	GAANNNNTTC	4 cut(s)	747, 936, 2337, 2845
ZrmI	AGTACT	1 cut(s)	264
Zsp2I	ATGCAT	2 cut(s)	1713, 2400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.