Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0011760

Physical Location & Seq

Genomic Coordinates Reverse (-)

265140 .. 269667

Gene Structure 4528 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1003800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2739 bp

ATGCCAGCCCTCAATCTGCTGATGATCCTCTTGCTCACGGCTGTCTACTTCTTCACCACCGCCACTTGTTACCACCACAACCGCCACCTCCTCCACCAGCCCTTTTACCCCTTTGAGTCCTCCCCTCCAACCCAACCCCCATCCTCCTCTCCCCAATCCCAGCCCTCCTCAATTCCCAAACTACCCTTCTCTTCCTCCTCCTCAACCCCTCCCCAAAAACCTTTCTTTCCCTCCTCCTCCGGCTCTCATTCCCCACTGCCCCCTCCCTCCCCAACTTCCCTCACCTCCTTCCCCGCCAACATTTCCTCTATCCTCTTTCCCAGAACCTCCTCCTCCTCCTCCTCCTCCCACCGCCACGTCATCGCCATCGCCGTCTCTGTTTCTGTCACTGCCTTCATCATCATCGCTTCCGCCGCCGCATTTCTGTACTACCGTCGAAGCCGGCAGCTTAACCCCACCTTATCCGACGATAAGGCCTCCAGACCCGACAGTCTGCGTCTGTTCCCGCCAAACACCGCCTCCTCCGACGCGGTTCACAAGCATCGTAGTAGTACGCCCAATACCAGCAGAGAGTTCCTCTACCTCGGCACCTTAGTTAGTCCCCGAGGAACTGACGAAGAAAACGCCGCGAACACGAGCAATGCGGGCTTGACGTTCGGGGGGTCGAGCCATTCTGGCTCGCCACCGTATCAGAAACTGGGCTCGCCGGAGCTGCAGCCGCTTCCGCCACTTCCCAAGCACAATTTCAGACGGAGCTTCAAGAATTCTCAACTGGGTTCCGACGACGAAGAGGAGTTCTTTTCTCCCAGAGGATCTTCCGCGAGTCCCAAACATGGTGGCGGAAGAATAACAAGCTCCGACAGAGTGTTTAAGGCCGTCGATGGCGTGAACTTCGGAAGCAGAAGCTTCAATTCGAGAACTGCATCATACCCATGTTCGAAATCTGCTTCCCCAGCAAGTTCTGGGTCTCGCACGGTCTCGCCAGGGTTGAATTTGAGCCCCATAAGCTTGAAATCGAAGTCGCCGGACTCAGTAATCAATTTCCCAGCTCCATGGCGGCCTCCTCAGATACCGGTATCATCGTCCCCATCATTATCCTCGTCGTCCTCAAGAAAATCTGACCTTTTCGGGCTGAAGAATCACTCTCCGGAGAGAAATAAAAGTTTTGTTCCGATAAGACTTTCTCCTCCGCCTCCTCCGCCTCCACCCCCGAGGTTTTGGGAAGCTCCAGCTCCGGTTGGTCGAAATCCGAACACAGGGCCACCGGTTCTTATCGCGCCTTCCCGGCCTGCTGTGCTTCAAACTTCGGGACCCATTTTGGCGGGTGAAAGTTCAGAGAGCAATGACAGAGTGGAAAAGAATGAGGAGACTCCAACAAAGCCAAAGCTGAAACCTTTACATTGGGATAAAGTTAGGGCCAGCTCAGACCACGCCATGGTGTGGGATCGCTTGAAATCCAGCTCTTTTCAGCTGAATGAAGAGATGATTGAGACATTGTTCATGGTAAACAATTCGGGTTTGGCGTCAAATGACAATGTCCGGCGACAGATTCTGCCCTCTGTGGATCAGGAGAACCGCGTGCTTGATCCGAAGAAGTCTCAGAACATTGCAATTTTGTTGAGGGCGCTTAATGTTACCCTTGATGAAGTCTGCGAGGCACTTTTGGAAGGTAATTCTGATGGACTTGGGACAGAACTTCTTGAAAGTCTATTAAAGATGGCTCCGACCAAAGAGGAAGAACGGAAACTAAGAGAGTTCAAAGATGAATCGCCGTTTAAGCTTGGCCCAGCTGAGAAATTTCTGAAGGCGGTGCTCGATATACCTTTCGCGTTTAAGAGGGTGGATGCAATGCTCTACATTGCCAATTTTGATTCAGAAGTTGATTATCTCAAAAGGTCTTTTGAGACTCTGGAGGCAGCTTGCGAAGAATTGAGGAACAGCAGAATGTTCCTCAAGCTTCTTGAAGCAGTGCTCAAAACTGGGAACCGCATGAATGTTGGCACTAACCGTGGCGATGCCCATGCCTTCAAGCTAGACACACTTCTCAAGCTTGTAGATGTGAAGGGAACAGATGGGAAGACTACTCTGTTACATTTTGTTGTGCAGGAAATCATCAGAGCTGAAGGTTCGCGTCTCTCTGGCATGAATCAGAATCAAACAACCGAGGATAGTCAACAATCTTCTTTCCGGGATGATGTTGAGTTTAGGAAGCTTGGCCTGCAAGTTGTTTCAGGATTGAGTGGGGAGCTCACCAGTGTAAAGAAGGCTGCTGCTATGGATTCAGATGTTCTTAGCAATGAAGTTGCGAAGATTGCTGGTGGAATTACAAAAATAGCAGAAGTAGTAAAATTAATTGAAGGAAGTGCATTGAAGGAGAGTAGCCAGAAATTCTTCGATTCCATGAGTGGGTTCTTGAACAAGGCTGGGGAAGAGATTGTAAGGATTCAAGCTCAAGAGAAAGTTGCATTCTCTATGGTAAAGGAATTAACAGAATATTTCCATGGGAACTCAGTTAGGGAAGAAGCTCATCCCTTCCGAATTTTCACGGTGGTGAGAGATTTTCTTTCTATTCTAGATCAGGTGTGCAAGGTGGTTGGAAAAGTTAATGAAAGAACTATCGTTGGGTCAGCCTGTCAGTTCCCCAGGGTTCCGAATGCAGCCCTTCCACCGGTTTTTCCAGGACTCAGTGCAAAGCAGCATGATGGTTACTCGGATGATGAAACCACAATTTCGACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009987 GO:0010638 GO:0010639 GO:0016020 GO:0016043 GO:0030029 GO:0030036 GO:0030312 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0030838 GO:0031333 GO:0031334 GO:0032271 GO:0032272 GO:0032273 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0043242 GO:0043244 GO:0043254 GO:0044087 GO:0044089 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051015 GO:0051016 GO:0051128 GO:0051129 GO:0051130 GO:0051493 GO:0051494 GO:0051495 GO:0051693 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0097435 GO:0110053 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04320 map04320

Pfam Domains

Protein Families

FH2

Protein Analysis

913

Amino Acids

99.57

Weight (kDa)

8.94

Isoelectric Point (pI)

60.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	455 - 858	1.4e-114	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0011760)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43800
fragaria_vesca	FvH4_1g00460
malus_domestica	MD02G1003800.v1.1 MD15G1147100.v1.1
prunus_persica	Prupe.7G267500_v2.0.a1
pyrus_communis	pycom02g00230 pycom15g13200
rosa_chinensis	RchiOBHm_Chr2g0084971
rosa_laevigata	RLG00000015652
rosa_multiflora	Rmu_co8206718.1_g000001 Rmu_sc0017508.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155670
rosa_rugosa	Rorug01G0457400
rosa_samantha	Rh2AG005000 Rh2BG006200 Rh2BG300200 Rh2CG006800 Rh2DG006600
rosa_wichuraiana	Rw2G000500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	489
AccB1I	GGYRCC	1 cut(s)	587
AccB7I	CCANNNNNTGG	2 cut(s)	1435, 1440
AccI	GTMKAC	1 cut(s)	45
AccII	CGCG	7 cut(s)	530, 629, 821, 1277, 1578, 1829, 2131
AccIII	TCCGGA	1 cut(s)	1147
AclWI	GGATC	5 cut(s)	19, 820, 1452, 1572, 1580
AcsI	RAATTY	5 cut(s)	763, 991, 1796, 2387, 2538
AcuI	CTGAAG	3 cut(s)	1154, 1823, 2142
AcyI	GRCGYC	1 cut(s)	1523
AfaI	GTAC	2 cut(s)	428, 553
AfiI	CCNNNNNNNGG	6 cut(s)	833, 1256, 1435, 1440, 2406, 2668
AgeI	ACCGGT	3 cut(s)	1072, 1264, 2668
AjiI	CACGTC	1 cut(s)	358
AjnI	CCWGG	3 cut(s)	982, 2642, 2677
AjuI	GAANNNNNNNTTGG	4 cut(s)	170, 202, 728, 760
AleI	CACNNNNGTG	1 cut(s)	2549
Alw21I	GWGCWC	3 cut(s)	1815, 1974, 2250
Alw26I	GTCTC	8 cut(s)	379, 972, 982, 1361, 1484, 1602, 1898, 2138
AlwI	GGATC	5 cut(s)	19, 820, 1452, 1572, 1580
AlwNI	CAGNNNCTG	2 cut(s)	697, 1552
Ama87I	CYCGRG	2 cut(s)	603, 1210
Aor13HI	TCCGGA	1 cut(s)	1147
AoxI	GGCC	8 cut(s)	474, 873, 1058, 1259, 1286, 1416, 1783, 2215
ApeKI	GCWGC	8 cut(s)	445, 712, 715, 1916, 2267, 2270, 2657, 2695
ApoI	RAATTY	5 cut(s)	763, 991, 1796, 2387, 2538
AseI	ATTAAT	1 cut(s)	2351
AsiGI	ACCGGT	3 cut(s)	1072, 1264, 2668
Asp700I	GAANNNNTTC	1 cut(s)	2390
AspLEI	GCGC	2 cut(s)	1279, 1627
AspS9I	GGNCC	4 cut(s)	1259, 1310, 1416, 1784
AsuC2I	CCSGG	2 cut(s)	1285, 2189
AsuHPI	GGTGA	5 cut(s)	46, 274, 1337, 2242, 2563
AsuII	TTCGAA	1 cut(s)	938
AvaI	CYCGRG	2 cut(s)	603, 1210
AvaII	GGWCC	1 cut(s)	1310
BanI	GGYRCC	1 cut(s)	587
BanII	GRGCYC	3 cut(s)	704, 1001, 2250
BauI	CACGAG	1 cut(s)	634
BbsI	GAAGAC	1 cut(s)	2084
Bbv12I	GWGCWC	3 cut(s)	1815, 1974, 2250
BbvI	GCAGC	8 cut(s)	457, 699, 727, 1928, 2254, 2257, 2669, 2707
BccI	CCATC	8 cut(s)	148, 374, 875, 1096, 1673, 1711, 2066, 2696
BceAI	ACGGC	4 cut(s)	54, 356, 860, 1756
BcgI	CGANNNNNNTGC	2 cut(s)	2003, 2037
BciT130I	CCWGG	3 cut(s)	984, 2644, 2679
BcnI	CCSGG	2 cut(s)	1285, 2189
BcoDI	GTCTC	8 cut(s)	379, 972, 982, 1361, 1484, 1602, 1898, 2138
BfaI	CTAG	2 cut(s)	2033, 2573
BfmI	CTRYAG	1 cut(s)	713
BfoI	RGCGCY	1 cut(s)	1628
BglI	GCCNNNNNGGC	2 cut(s)	675, 1285
Bme1390I	CCNGG	5 cut(s)	984, 1285, 2189, 2644, 2679
Bme18I	GGWCC	1 cut(s)	1310
BmeT110I	CYCGRG	2 cut(s)	603, 1210
BmgBI	CACGTC	1 cut(s)	358
BmgT120I	GGNCC	4 cut(s)	1259, 1310, 1416, 1784
BmiI	GGNNCC	7 cut(s)	589, 778, 1311, 1312, 1722, 1985, 2649
BmrFI	CCNGG	5 cut(s)	984, 1285, 2189, 2644, 2679
BmrI	ACTGGG	3 cut(s)	707, 782, 1989
BmsI	GCATC	4 cut(s)	550, 932, 1834, 2005
BmuI	ACTGGG	3 cut(s)	707, 782, 1989
BpiI	GAAGAC	1 cut(s)	2084
BplI	GAGNNNNNCTC	2 cut(s)	561, 593
BpmI	CTGGAG	3 cut(s)	463, 1212, 1931
Bpu14I	TTCGAA	1 cut(s)	938
BpuEI	CTTGAG	4 cut(s)	1093, 1937, 2030, 2436
BpuMI	CCSGG	2 cut(s)	1285, 2189
BsaHI	GRCGYC	1 cut(s)	1523
BsaI	GGTCTC	2 cut(s)	972, 982
BsaWI	WCCGGW	5 cut(s)	1072, 1147, 1234, 1264, 2668
BsaXI	ACNNNNNCTCC	2 cut(s)	503, 533
Bsc4I	CCNNNNNNNGG	6 cut(s)	833, 1256, 1435, 1440, 2406, 2668
Bse118I	RCCGGY	4 cut(s)	441, 1072, 1264, 2668
Bse1I	ACTGG	4 cut(s)	702, 777, 1984, 2253
Bse3DI	GCAATG	6 cut(s)	646, 1348, 1605, 1854, 1857, 2302
BseAI	TCCGGA	1 cut(s)	1147
BseBI	CCWGG	3 cut(s)	984, 2644, 2679
BseGI	GGATG	5 cut(s)	140, 1849, 2197, 2527, 2719
BseLI	CCNNNNNNNGG	6 cut(s)	833, 1256, 1435, 1440, 2406, 2668
BseMI	GCAATG	6 cut(s)	646, 1348, 1605, 1854, 1857, 2302
BseMII	CTCAG	7 cut(s)	1044, 1079, 1437, 1613, 1782, 2523, 2698
BseNI	ACTGG	4 cut(s)	702, 777, 1984, 2253
BseXI	GCAGC	8 cut(s)	457, 699, 727, 1928, 2254, 2257, 2669, 2707
BseYI	CCCAGC	5 cut(s)	159, 952, 1045, 1786, 2423
BsgI	GTGCAG	1 cut(s)	2123
Bsh1236I	CGCG	7 cut(s)	530, 629, 821, 1277, 1578, 1829, 2131
BshFI	GGCC	8 cut(s)	476, 875, 1060, 1261, 1288, 1418, 1785, 2217
BshNI	GGYRCC	1 cut(s)	587
BshTI	ACCGGT	3 cut(s)	1072, 1264, 2668
BsiHKAI	GWGCWC	3 cut(s)	1815, 1974, 2250
BsiHKCI	CYCGRG	2 cut(s)	603, 1210
BslFI	GGGAC	5 cut(s)	585, 810, 1069, 1323, 1702
BslI	CCNNNNNNNGG	6 cut(s)	833, 1256, 1435, 1440, 2406, 2668
BsmAI	GTCTC	8 cut(s)	379, 972, 982, 1361, 1484, 1602, 1898, 2138
BsmBI	CGTCTC	2 cut(s)	379, 2138
BsmFI	GGGAC	5 cut(s)	585, 810, 1069, 1323, 1702
BsmI	GAATGC	2 cut(s)	2465, 2659
BsnI	GGCC	8 cut(s)	476, 875, 1060, 1261, 1288, 1418, 1785, 2217
Bso31I	GGTCTC	2 cut(s)	972, 982
BsoBI	CYCGRG	2 cut(s)	603, 1210
Bsp119I	TTCGAA	1 cut(s)	938
Bsp1286I	GDGCHC	5 cut(s)	704, 1001, 1815, 1974, 2250
Bsp13I	TCCGGA	1 cut(s)	1147
Bsp143I	GATC	6 cut(s)	24, 812, 1444, 1564, 1585, 2575
Bsp19I	CCATGG	3 cut(s)	1052, 1434, 2500
BspANI	GGCC	8 cut(s)	476, 875, 1060, 1261, 1288, 1418, 1785, 2217
BspCNI	CTCAG	7 cut(s)	1043, 1078, 1436, 1612, 1783, 2522, 2697
BspEI	TCCGGA	1 cut(s)	1147
BspFNI	CGCG	7 cut(s)	530, 629, 821, 1277, 1578, 1829, 2131
BspLI	GGNNCC	7 cut(s)	589, 778, 1311, 1312, 1722, 1985, 2649
BspMAI	CTGCAG	1 cut(s)	717
BspPI	GGATC	5 cut(s)	19, 820, 1452, 1572, 1580
BspT104I	TTCGAA	1 cut(s)	938
BspT107I	GGYRCC	1 cut(s)	587
BspTNI	GGTCTC	2 cut(s)	972, 982
BsrDI	GCAATG	6 cut(s)	646, 1348, 1605, 1854, 1857, 2302
BsrFI	RCCGGY	4 cut(s)	441, 1072, 1264, 2668
BsrI	ACTGG	4 cut(s)	702, 777, 1984, 2253
BssAI	RCCGGY	4 cut(s)	441, 1072, 1264, 2668
BssMI	GATC	6 cut(s)	24, 812, 1444, 1564, 1585, 2575
BssNI	GRCGYC	1 cut(s)	1523
BssSI	CACGAG	1 cut(s)	634
BssT1I	CCWWGG	3 cut(s)	1052, 1434, 2500
Bst2BI	CACGAG	1 cut(s)	634
Bst2UI	CCWGG	3 cut(s)	984, 2644, 2679
Bst4CI	ACNGT	6 cut(s)	434, 491, 687, 976, 2009, 2548
Bst6I	CTCTTC	4 cut(s)	196, 783, 1473, 2424
BstACI	GRCGYC	1 cut(s)	1523
BstBI	TTCGAA	1 cut(s)	938
BstDSI	CCRYGG	4 cut(s)	1052, 1434, 2008, 2500
BstF5I	GGATG	5 cut(s)	140, 1849, 2197, 2527, 2719
BstFNI	CGCG	7 cut(s)	530, 629, 821, 1277, 1578, 1829, 2131
BstH2I	RGCGCY	1 cut(s)	1628
BstHHI	GCGC	2 cut(s)	1279, 1627
BstKTI	GATC	6 cut(s)	27, 815, 1447, 1567, 1588, 2578
BstMAI	GTCTC	8 cut(s)	379, 972, 982, 1361, 1484, 1602, 1898, 2138
BstMBI	GATC	6 cut(s)	24, 812, 1444, 1564, 1585, 2575
BstNI	CCWGG	3 cut(s)	984, 2644, 2679
BstSCI	CCNGG	5 cut(s)	982, 1283, 2187, 2642, 2677
BstSFI	CTRYAG	1 cut(s)	713
BstUI	CGCG	7 cut(s)	530, 629, 821, 1277, 1578, 1829, 2131
BstV1I	GCAGC	8 cut(s)	457, 699, 727, 1928, 2254, 2257, 2669, 2707
BstV2I	GAAGAC	1 cut(s)	2084
BstX2I	RGATCY	1 cut(s)	812
BstXI	CCANNNNNNTGG	1 cut(s)	1053
BstYI	RGATCY	1 cut(s)	812
BsuRI	GGCC	8 cut(s)	476, 875, 1060, 1261, 1288, 1418, 1785, 2217
BtgI	CCRYGG	4 cut(s)	1052, 1434, 2008, 2500
BtgZI	GCGATG	4 cut(s)	346, 352, 388, 2028
BtrI	CACGTC	1 cut(s)	358
BtsCI	GGATG	5 cut(s)	140, 1849, 2197, 2527, 2719
BtsI	GCAGTG	3 cut(s)	254, 387, 1974
BtsIMutI	CAGTG	5 cut(s)	254, 387, 1974, 2260, 2692
CaiI	CAGNNNCTG	2 cut(s)	697, 1552
CfoI	GCGC	2 cut(s)	1279, 1627
Cfr10I	RCCGGY	4 cut(s)	441, 1072, 1264, 2668
Cfr13I	GGNCC	4 cut(s)	1259, 1310, 1416, 1784
CseI	GACGC	4 cut(s)	485, 536, 1512, 2120
Csp6I	GTAC	2 cut(s)	427, 552
CspAI	ACCGGT	3 cut(s)	1072, 1264, 2668
CspCI	CAANNNNNGTGG	2 cut(s)	817, 852
CviQI	GTAC	2 cut(s)	427, 552
DpnI	GATC	6 cut(s)	26, 814, 1446, 1566, 1587, 2577
DpnII	GATC	6 cut(s)	24, 812, 1444, 1564, 1585, 2575
DrdI	GACNNNNNNGTC	1 cut(s)	489
DseDI	GACNNNNNNGTC	1 cut(s)	489
Eam1104I	CTCTTC	4 cut(s)	196, 783, 1473, 2424
EarI	CTCTTC	4 cut(s)	196, 783, 1473, 2424
EciI	GGCGGA	5 cut(s)	400, 714, 855, 1179, 1188
Ecl136II	GAGCTC	1 cut(s)	2248
Eco130I	CCWWGG	3 cut(s)	1052, 1434, 2500
Eco147I	AGGCCT	1 cut(s)	476
Eco24I	GRGCYC	3 cut(s)	704, 1001, 2250
Eco31I	GGTCTC	2 cut(s)	972, 982
Eco47I	GGWCC	1 cut(s)	1310
Eco53kI	GAGCTC	1 cut(s)	2248
Eco57I	CTGAAG	3 cut(s)	1154, 1823, 2142
Eco88I	CYCGRG	2 cut(s)	603, 1210
EcoICRI	GAGCTC	1 cut(s)	2248
EcoO109I	RGGNCCY	1 cut(s)	1310
EcoRI	GAATTC	1 cut(s)	763
EcoRII	CCWGG	3 cut(s)	982, 2642, 2677
EcoT14I	CCWWGG	3 cut(s)	1052, 1434, 2500
EcoT38I	GRGCYC	3 cut(s)	704, 1001, 2250
ErhI	CCWWGG	3 cut(s)	1052, 1434, 2500
Esp3I	CGTCTC	2 cut(s)	379, 2138
FaqI	GGGAC	5 cut(s)	585, 810, 1069, 1323, 1702
FauI	CCCGC	4 cut(s)	301, 513, 637, 1315
FblI	GTMKAC	1 cut(s)	45
FokI	GGATG	5 cut(s)	127, 1856, 2204, 2514, 2726
FriOI	GRGCYC	3 cut(s)	704, 1001, 2250
FspBI	CTAG	2 cut(s)	2033, 2573
GlaI	GCGC	2 cut(s)	1278, 1626
GsaI	CCCAGC	5 cut(s)	163, 956, 1049, 1790, 2427
GsuI	CTGGAG	3 cut(s)	463, 1212, 1931
HaeII	RGCGCY	1 cut(s)	1628
HaeIII	GGCC	8 cut(s)	476, 875, 1060, 1261, 1288, 1418, 1785, 2217
HgaI	GACGC	4 cut(s)	485, 536, 1512, 2120
HhaI	GCGC	2 cut(s)	1279, 1627
Hin1I	GRCGYC	1 cut(s)	1523
Hin6I	GCGC	2 cut(s)	1277, 1625
HinP1I	GCGC	2 cut(s)	1277, 1625
HincII	GTYRAC	1 cut(s)	2174
HindII	GTYRAC	1 cut(s)	2174
HindIII	AAGCTT	6 cut(s)	904, 1006, 1778, 1955, 2048, 2210
HphI	GGTGA	5 cut(s)	46, 274, 1337, 2242, 2563
Hpy166II	GTNNAC	5 cut(s)	46, 535, 889, 1507, 2174
Hpy8I	GTNNAC	5 cut(s)	46, 535, 889, 1507, 2174
Hpy99I	CGWCG	7 cut(s)	438, 470, 530, 785, 788, 881, 1105
HpyCH4III	ACNGT	6 cut(s)	434, 491, 687, 976, 2009, 2548
HpyCH4IV	ACGT	2 cut(s)	357, 653
HpySE526I	ACGT	2 cut(s)	357, 653
Hsp92I	GRCGYC	1 cut(s)	1523
HspAI	GCGC	2 cut(s)	1277, 1625
KflI	GGGWCCC	1 cut(s)	1310
Kpn2I	TCCGGA	1 cut(s)	1147
KroI	GCCGGC	1 cut(s)	441
KroNI	GCCGGC	1 cut(s)	443
Kzo9I	GATC	6 cut(s)	24, 812, 1444, 1564, 1585, 2575
LmnI	GCTCC	8 cut(s)	709, 753, 860, 1054, 1231, 1237, 1726, 2245
Lsp1109I	GCAGC	8 cut(s)	457, 699, 727, 1928, 2254, 2257, 2669, 2707
LweI	GCATC	4 cut(s)	550, 932, 1834, 2005
MaeI	CTAG	2 cut(s)	2033, 2573
MaeII	ACGT	2 cut(s)	357, 653
MaeIII	GTNAC	5 cut(s)	68, 385, 1633, 2088, 2705
MalI	GATC	6 cut(s)	26, 814, 1446, 1566, 1587, 2577
MboI	GATC	6 cut(s)	24, 812, 1444, 1564, 1585, 2575
MflI	RGATCY	1 cut(s)	812
MhlI	GDGCHC	5 cut(s)	704, 1001, 1815, 1974, 2250
MlyI	GAGTC	6 cut(s)	125, 832, 1022, 1363, 1900, 2676
MmeI	TCCRAC	8 cut(s)	152, 489, 549, 804, 882, 1397, 1748, 2575
MroI	TCCGGA	1 cut(s)	1147
MroNI	GCCGGC	1 cut(s)	441
MroXI	GAANNNNTTC	1 cut(s)	2390
MseI	TTAA	9 cut(s)	450, 870, 1629, 1712, 1776, 1833, 2351, 2486, 2604
MslI	CAYNNNNRTG	2 cut(s)	931, 2549
MspA1I	CMGCKG	2 cut(s)	1471, 1790
MspR9I	CCNGG	5 cut(s)	984, 1285, 2189, 2644, 2679
Mva1269I	GAATGC	2 cut(s)	2465, 2659
MvaI	CCWGG	3 cut(s)	984, 2644, 2679
MvnI	CGCG	7 cut(s)	530, 629, 821, 1277, 1578, 1829, 2131
NaeI	GCCGGC	1 cut(s)	443
NciI	CCSGG	2 cut(s)	1285, 2189
NcoI	CCATGG	3 cut(s)	1052, 1434, 2500
NdeII	GATC	6 cut(s)	24, 812, 1444, 1564, 1585, 2575
NgoMIV	GCCGGC	1 cut(s)	441
NlaIV	GGNNCC	7 cut(s)	589, 778, 1311, 1312, 1722, 1985, 2649
NmeAIII	GCCGAG	1 cut(s)	564
NmuCI	GTSAC	1 cut(s)	385
NspV	TTCGAA	1 cut(s)	938
OliI	CACNNNNGTG	1 cut(s)	2549
PasI	CCCWGGG	1 cut(s)	2643
PceI	AGGCCT	1 cut(s)	476
PcsI	WCGNNNNNNNCGW	2 cut(s)	369, 1520
PctI	GAATGC	2 cut(s)	2465, 2659
PdiI	GCCGGC	1 cut(s)	443
PdmI	GAANNNNTTC	1 cut(s)	2390
PfeI	GAWTC	9 cut(s)	1138, 1549, 1766, 1871, 2146, 2152, 2279, 2396, 2443
PflFI	GACNNNGTC	1 cut(s)	1535
PflMI	CCANNNNNTGG	2 cut(s)	1435, 1440
PfoI	TCCNGGA	2 cut(s)	2187, 2677
PinAI	ACCGGT	3 cut(s)	1072, 1264, 2668
PleI	GAGTC	6 cut(s)	124, 831, 1022, 1363, 1900, 2676
PpsI	GAGTC	6 cut(s)	124, 831, 1022, 1363, 1900, 2676
PpuMI	RGGWCCY	1 cut(s)	1310
PshBI	ATTAAT	1 cut(s)	2351
Psp124BI	GAGCTC	1 cut(s)	2250
Psp5II	RGGWCCY	1 cut(s)	1310
Psp6I	CCWGG	3 cut(s)	982, 2642, 2677
PspFI	CCCAGC	5 cut(s)	159, 952, 1045, 1786, 2423
PspGI	CCWGG	3 cut(s)	982, 2642, 2677
PspN4I	GGNNCC	7 cut(s)	589, 778, 1311, 1312, 1722, 1985, 2649
PspPI	GGNCC	4 cut(s)	1259, 1310, 1416, 1784
PspPPI	RGGWCCY	1 cut(s)	1310
PstI	CTGCAG	1 cut(s)	717
PstNI	CAGNNNCTG	2 cut(s)	697, 1552
PsuI	RGATCY	1 cut(s)	812
PsyI	GACNNNGTC	1 cut(s)	1535
PvuII	CAGCTG	2 cut(s)	1471, 1790
RsaI	GTAC	2 cut(s)	428, 553
RsaNI	GTAC	2 cut(s)	427, 552
RseI	CAYNNNNRTG	2 cut(s)	931, 2549
SacI	GAGCTC	1 cut(s)	2250
SaqAI	TTAA	9 cut(s)	450, 870, 1629, 1712, 1776, 1833, 2351, 2486, 2604
Sau3AI	GATC	6 cut(s)	24, 812, 1444, 1564, 1585, 2575
Sau96I	GGNCC	4 cut(s)	1259, 1310, 1416, 1784
SchI	GAGTC	6 cut(s)	125, 832, 1022, 1363, 1900, 2676
ScrFI	CCNGG	5 cut(s)	984, 1285, 2189, 2644, 2679
SduI	GDGCHC	5 cut(s)	704, 1001, 1815, 1974, 2250
SfaNI	GCATC	4 cut(s)	550, 932, 1834, 2005
SfcI	CTRYAG	1 cut(s)	713
SfuI	TTCGAA	1 cut(s)	938
SinI	GGWCC	1 cut(s)	1310
SmiMI	CAYNNNNRTG	2 cut(s)	931, 2549
SmlI	CTYRAG	4 cut(s)	1108, 1952, 2045, 2451
SmoI	CTYRAG	4 cut(s)	1108, 1952, 2045, 2451
SseBI	AGGCCT	1 cut(s)	476
SspI	AATATT	1 cut(s)	2495
SspMI	CTAG	2 cut(s)	2033, 2573
SstI	GAGCTC	1 cut(s)	2250
StuI	AGGCCT	1 cut(s)	476
StyD4I	CCNGG	5 cut(s)	982, 1283, 2187, 2642, 2677
StyI	CCWWGG	3 cut(s)	1052, 1434, 2500
TaaI	ACNGT	6 cut(s)	434, 491, 687, 976, 2009, 2548
TaiI	ACGT	2 cut(s)	360, 656
TatI	WGTACW	1 cut(s)	426
TauI	GCSGC	5 cut(s)	416, 419, 629, 721, 1060
TfiI	GAWTC	9 cut(s)	1138, 1549, 1766, 1871, 2146, 2152, 2279, 2396, 2443
Tru1I	TTAA	9 cut(s)	450, 870, 1629, 1712, 1776, 1833, 2351, 2486, 2604
Tru9I	TTAA	9 cut(s)	450, 870, 1629, 1712, 1776, 1833, 2351, 2486, 2604
TscAI	CASTG	5 cut(s)	261, 394, 1974, 2260, 2692
TseFI	GTSAC	1 cut(s)	385
TseI	GCWGC	8 cut(s)	445, 712, 715, 1916, 2267, 2270, 2657, 2695
Tsp45I	GTSAC	1 cut(s)	385
TspGWI	ACGGA	2 cut(s)	766, 1756
TspRI	CASTG	5 cut(s)	261, 394, 1974, 2260, 2692
Tth111I	GACNNNGTC	1 cut(s)	1535
Van91I	CCANNNNNTGG	2 cut(s)	1435, 1440
VpaK11BI	GGWCC	1 cut(s)	1310
VspI	ATTAAT	1 cut(s)	2351
XapI	RAATTY	5 cut(s)	763, 991, 1796, 2387, 2538
XbaI	TCTAGA	1 cut(s)	2572
XmiI	GTMKAC	1 cut(s)	45
XmnI	GAANNNNTTC	1 cut(s)	2390
XspI	CTAG	2 cut(s)	2033, 2573

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.