Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0011760

Physical Location & Seq

Genomic Coordinates Reverse (-)

136880 .. 140630

Gene Structure 3751 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g00230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2733 bp

ATGCCAGCCCTCAATCCGCTGATGATACTCTTGCTCACGACTGTCTACTTCTTCACCACCGCCACTTGTTACCACCACAACCGCCACCTCCTCCACCAGCCCCTTTTCCCCTCTGAGTCCTCCCCTCCAACCCAACCCCCATCCTCCTCTCCCCAATCCCTGCCCACCCCAATTCCCAAACTACCCTTCTCTTCCTCCTCCTCAACCCCTCCCCAAAAACCTTTCTTTCCCTCCGCCTCCCGCTCTCATTCCCCACTGCCCCCTCCCTCCCCAACTTCCCTCACCTCCTTCCCCGCCAACATTTCTTCTATCCTCTTTCCCAGAACCTCCTCCTCCTCCTCCTCCTCCCACCGCCACGTCATCGCCATCGCCGTCTCTGTTTCTATCACTGCCTTCATCATCATCGCCTCCGCCGCCGCATTTCTGTACTACCGTCGAAGCCGGCAGCTTAACCCCACCTTATCCGACGATAAGGCCTCCAGACCCGACAGTCTGCGTCTGTTCCCGCCAAACACCGCCTCCTCCGACGCGGTTCACAAGCATTGTAGTAGTACGCCCAATACCAGCAAAGAGTTCCTCTACCTCGGCACCTTAGTTAGTCCCCGAGGAACTGACGAAGAAAACGCCGCGAACACGAGCAATGCGGGCTTGACGTTCGGGGGGTCGAGCCATTCTGCCTCGCCACCGTATCAGAAACTGGGCTCGCCGGAGCTGCAGCCGCTTCCACCACTTCCCAAGCACAATTTCAGACGGAGCTTCAAGAATTCCCAACTGGGTTCCGACGACGAAGAGGAGTTCTTTTCTCCCAGAGGATCTTCCGCGAGTCCCAAACATGGTGGCGGAAGAATAACAAGCTCCGACAGAGTGTTGAAGGCCGTCGATGGCGTCAACTTCGGAAGCAGAAGCTTCAATTCGAGAACTGCATCATACCCATGTTCGAAATCTGCTTCCCCAGCAAGTTCTGGGTCTCGCACGGTCTCGCCAGCGTTGAATATGAGCCCCATAAGCTTGAAATCGAAGTCCCCGGACTCAGTAATCAATTTCCCAGCTCCATGGCGGCCTCCTCAGATACCGGTATCATCGTCCCCATCATTATCCTCGTCGTCCTCAAGAAAATCTGACCTTTTCGGGCTGAAGAATCACTCTCCGGAGAGAAATAAAAGTTTTGTTCCGATCAGACTTTCTCCTCCGCCTCCTCCGCCGCCACCCCCGAGGTTTTGGGAAGCTCCGGTTGGTCGAACTCCGAACACAGGGCCACCGGTTCTTATCACGCCTTCCCGGCCTGCTGTGGTTCAAACTTCGGGACCCATTTTGGCGGGTGAAAGTTCAGAGAGCAATGAGATGGTGGAAAAGAATGAGGAGACTCCAACAAAACCAAAGCTGAAACCTTTACATTGGGATAAAGTAAGGGCCAGCTCAGACCACGCCATGGTCTGGGATCGCTTGAAATCCAGCTCTTTTCAGCTGAATGAGGAGATGATCGAGACATTGTTCGTGGTAAACAATTCGGGTTTGGCACCAAATGACAATGTCCGGCGACAGATTCTGCCTTCTGTGGATCAGGAGAACCGCGTGATCGATCCGAAGAAGTCTCAGAACATTGCAATTTTGTTGAGGGCGCTTAATGTTACCCTTGATGAAGTCTGTGAGGCGCTTTTGGAAGGTAATTCTGATGGACTTGGGACAGAGCTGCTTGAAAGTCTATTAAAGATGGCTCCGACCAAAGAGGAAGAACGGAAACTAAGAGAGTTCAAAGATGAATCGCCGTTTAAGCTTGGCCCAGCCGAGAAATTTCTGAAGGCGGTGCTCGATATACCTTTCGCGTTTAAGAGGGTGGATGCAATGCTCTACATTGCCAATTTTGATTCAGAAGTTGATTATCTCAAAAGGTCTTTTGAGACTCTGGAGGCAGCTTGCGAAGAATTGAGGAACAGCAGAATGTTCCTCAAGCTTCTTGAAGCAGTGCTCAAAACTGGGAACCGCATGAATGTTGGCACTAACCGTGGCGATGCCCATGCCTTCAAGCTAGACACACTTCTCAAGCTTGTAGACGTGAAGGGAACAGATGGGAAAACCACTCTGTTACATTTTGTTGTGCAGGAAATCATCAGAGCTGAAGGTTCGCGTCTCTCTGGCATGAATCAGAATCGAACAACCGAGGATAGTCAACAATCTTCTTTCCGGGATGATGTTGAGTTTAGGAAGCTTGGCCTGCAAGTTGTTTCAGGATTGAGTGGGGAGCTCACCAGTGTAAAGAAGGCTGCTGCTATGGATTCAGATGTTCTTAGCAATGAAGTTGCGAAAATTGCTGGTGGAATTACAAAAATAGCAGAAGTAGTAAAATTAATTGAAGGAAGTGCATCGAAGGAGAGTAGCCAGAAATTCTTCGATTCCATGAGTGGGTTCTTGAACAAGGCTGGGGAAGAGATTGTAAGGATTCAAGCTCAAGAGAAAGTTGCATTCTCTATGGTAAAGGAATTAACAGAATATTTCCATGGGAGCTCAGTTAGGGAAGAAGCTCATCCCTTCCGAATTTTCACAGTGGTGAGAGATTTTCTTTCTATTCTAGATCAGGTGTGCAAGGTGGTTGGAAAAGTTAATGAAAGAACTATCGTTGGGTCAGCATGTCAGTTCCCCAGGGTTCCGAATGCAACCCTTCCACCGGTTTTTCCAGGACTCGGTGCAAAGCAGCATGATGGTTACTCGGATGATGAAACCGCGATTTCGACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009987 GO:0010638 GO:0010639 GO:0016020 GO:0016043 GO:0030029 GO:0030036 GO:0030312 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0030838 GO:0031333 GO:0031334 GO:0032271 GO:0032272 GO:0032273 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0043242 GO:0043244 GO:0043254 GO:0044087 GO:0044089 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051015 GO:0051016 GO:0051128 GO:0051129 GO:0051130 GO:0051493 GO:0051494 GO:0051495 GO:0051693 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0097435 GO:0110053 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04320 map04320

Pfam Domains

Protein Families

FH2

Protein Analysis

911

Amino Acids

99.25

Weight (kDa)

8.91

Isoelectric Point (pI)

59.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	453 - 856	5.1e-115	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011760)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43800
fragaria_vesca	FvH4_1g00460
malus_domestica	MD02G1003800.v1.1 MD15G1147100.v1.1
prunus_persica	Prupe.7G267500_v2.0.a1
pyrus_communis	pycom02g00230 pycom15g13200
rosa_chinensis	RchiOBHm_Chr2g0084971
rosa_laevigata	RLG00000015652
rosa_multiflora	Rmu_co8206718.1_g000001 Rmu_sc0017508.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155670
rosa_rugosa	Rorug01G0457400
rosa_samantha	Rh2AG005000 Rh2BG006200 Rh2BG300200 Rh2CG006800 Rh2DG006600
rosa_wichuraiana	Rw2G000500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	489
AccB1I	GGYRCC	2 cut(s)	587, 1516
AccB7I	CCANNNNNTGG	2 cut(s)	1429, 1434
AccBSI	CCGCTC	1 cut(s)	243
AccI	GTMKAC	2 cut(s)	45, 2049
AccII	CGCG	7 cut(s)	530, 629, 821, 1572, 1823, 2125, 2720
AccIII	TCCGGA	1 cut(s)	1147
AclWI	GGATC	4 cut(s)	820, 1446, 1566, 1574
AcsI	RAATTY	4 cut(s)	763, 1790, 2381, 2532
AcuI	CTGAAG	3 cut(s)	1154, 1817, 2136
AcyI	GRCGYC	1 cut(s)	885
AfaI	GTAC	2 cut(s)	428, 553
AfiI	CCNNNNNNNGG	7 cut(s)	833, 1250, 1429, 1434, 2400, 2662, 2678
AgeI	ACCGGT	3 cut(s)	1072, 1258, 2662
AjiI	CACGTC	2 cut(s)	358, 2053
AjnI	CCWGG	2 cut(s)	2636, 2671
AjuI	GAANNNNNNNTTGG	6 cut(s)	170, 202, 728, 760, 1215, 1247
AleI	CACNNNNGTG	1 cut(s)	2543
Alw21I	GWGCWC	4 cut(s)	1809, 1968, 2244, 2504
Alw26I	GTCTC	8 cut(s)	379, 972, 982, 1355, 1478, 1596, 1892, 2132
AlwI	GGATC	4 cut(s)	820, 1446, 1566, 1574
AlwNI	CAGNNNCTG	2 cut(s)	697, 1546
Ama87I	CYCGRG	2 cut(s)	603, 1210
Aor13HI	TCCGGA	1 cut(s)	1147
AoxI	GGCC	8 cut(s)	474, 873, 1058, 1253, 1280, 1410, 1777, 2209
ApeKI	GCWGC	8 cut(s)	445, 712, 715, 1690, 1910, 2261, 2264, 2689
ApoI	RAATTY	4 cut(s)	763, 1790, 2381, 2532
AseI	ATTAAT	1 cut(s)	2345
AsiGI	ACCGGT	3 cut(s)	1072, 1258, 2662
Asp700I	GAANNNNTTC	1 cut(s)	2384
AspLEI	GCGC	2 cut(s)	1621, 1654
AspS9I	GGNCC	4 cut(s)	1253, 1304, 1410, 1778
AsuC2I	CCSGG	3 cut(s)	1025, 1279, 2183
AsuHPI	GGTGA	5 cut(s)	46, 274, 1331, 2236, 2557
AsuII	TTCGAA	1 cut(s)	938
AvaI	CYCGRG	2 cut(s)	603, 1210
AvaII	GGWCC	1 cut(s)	1304
BanI	GGYRCC	2 cut(s)	587, 1516
BanII	GRGCYC	4 cut(s)	704, 1001, 2244, 2504
BauI	CACGAG	1 cut(s)	634
Bbv12I	GWGCWC	4 cut(s)	1809, 1968, 2244, 2504
BbvI	GCAGC	8 cut(s)	457, 699, 727, 1677, 1922, 2248, 2251, 2701
BccI	CCATC	9 cut(s)	148, 374, 875, 1096, 1336, 1667, 1705, 2060, 2690
BceAI	ACGGC	3 cut(s)	356, 860, 1750
BcgI	CGANNNNNNTGC	2 cut(s)	1997, 2031
BciT130I	CCWGG	2 cut(s)	2638, 2673
BcnI	CCSGG	3 cut(s)	1025, 1279, 2183
BcoDI	GTCTC	8 cut(s)	379, 972, 982, 1355, 1478, 1596, 1892, 2132
BfaI	CTAG	2 cut(s)	2027, 2567
BfmI	CTRYAG	1 cut(s)	713
BfoI	RGCGCY	2 cut(s)	1622, 1655
BglI	GCCNNNNNGGC	1 cut(s)	1279
Bme1390I	CCNGG	5 cut(s)	1025, 1279, 2183, 2638, 2673
Bme18I	GGWCC	1 cut(s)	1304
BmeT110I	CYCGRG	2 cut(s)	603, 1210
BmgBI	CACGTC	2 cut(s)	358, 2053
BmgT120I	GGNCC	4 cut(s)	1253, 1304, 1410, 1778
BmiI	GGNNCC	8 cut(s)	589, 778, 1305, 1306, 1518, 1716, 1979, 2643
BmrFI	CCNGG	5 cut(s)	1025, 1279, 2183, 2638, 2673
BmrI	ACTGGG	3 cut(s)	707, 782, 1983
BmsI	GCATC	4 cut(s)	932, 1828, 1999, 2369
BmuI	ACTGGG	3 cut(s)	707, 782, 1983
BpmI	CTGGAG	2 cut(s)	463, 1925
Bpu14I	TTCGAA	1 cut(s)	938
BpuEI	CTTGAG	4 cut(s)	1093, 1931, 2024, 2430
BpuMI	CCSGG	3 cut(s)	1025, 1279, 2183
Bsa29I	ATCGAT	1 cut(s)	1578
BsaHI	GRCGYC	1 cut(s)	885
BsaI	GGTCTC	2 cut(s)	972, 982
BsaWI	WCCGGW	5 cut(s)	1072, 1147, 1228, 1258, 2662
BsaXI	ACNNNNNCTCC	2 cut(s)	503, 533
Bsc4I	CCNNNNNNNGG	7 cut(s)	833, 1250, 1429, 1434, 2400, 2662, 2678
Bse118I	RCCGGY	4 cut(s)	441, 1072, 1258, 2662
Bse1I	ACTGG	4 cut(s)	702, 777, 1978, 2247
Bse3DI	GCAATG	6 cut(s)	646, 1342, 1599, 1848, 1851, 2296
BseAI	TCCGGA	1 cut(s)	1147
BseBI	CCWGG	2 cut(s)	2638, 2673
BseCI	ATCGAT	1 cut(s)	1578
BseGI	GGATG	5 cut(s)	140, 1843, 2191, 2521, 2713
BseLI	CCNNNNNNNGG	7 cut(s)	833, 1250, 1429, 1434, 2400, 2662, 2678
BseMI	GCAATG	6 cut(s)	646, 1342, 1599, 1848, 1851, 2296
BseMII	CTCAG	6 cut(s)	105, 1044, 1079, 1431, 1607, 2517
BseNI	ACTGG	4 cut(s)	702, 777, 1978, 2247
BseXI	GCAGC	8 cut(s)	457, 699, 727, 1677, 1922, 2248, 2251, 2701
BseYI	CCCAGC	4 cut(s)	952, 1045, 1780, 2417
BsgI	GTGCAG	1 cut(s)	2117
Bsh1236I	CGCG	7 cut(s)	530, 629, 821, 1572, 1823, 2125, 2720
BshFI	GGCC	8 cut(s)	476, 875, 1060, 1255, 1282, 1412, 1779, 2211
BshNI	GGYRCC	2 cut(s)	587, 1516
BshTI	ACCGGT	3 cut(s)	1072, 1258, 2662
BshVI	ATCGAT	1 cut(s)	1578
BsiHKAI	GWGCWC	4 cut(s)	1809, 1968, 2244, 2504
BsiHKCI	CYCGRG	2 cut(s)	603, 1210
BslFI	GGGAC	6 cut(s)	585, 810, 1006, 1069, 1317, 1696
BslI	CCNNNNNNNGG	7 cut(s)	833, 1250, 1429, 1434, 2400, 2662, 2678
BsmAI	GTCTC	8 cut(s)	379, 972, 982, 1355, 1478, 1596, 1892, 2132
BsmBI	CGTCTC	2 cut(s)	379, 2132
BsmFI	GGGAC	6 cut(s)	585, 810, 1006, 1069, 1317, 1696
BsmI	GAATGC	2 cut(s)	2459, 2653
BsnI	GGCC	8 cut(s)	476, 875, 1060, 1255, 1282, 1412, 1779, 2211
Bso31I	GGTCTC	2 cut(s)	972, 982
BsoBI	CYCGRG	2 cut(s)	603, 1210
Bsp119I	TTCGAA	1 cut(s)	938
Bsp1286I	GDGCHC	6 cut(s)	704, 1001, 1809, 1968, 2244, 2504
Bsp13I	TCCGGA	1 cut(s)	1147
Bsp143I	GATC	8 cut(s)	812, 1173, 1438, 1479, 1558, 1575, 1579, 2569
Bsp19I	CCATGG	3 cut(s)	1052, 1428, 2494
BspANI	GGCC	8 cut(s)	476, 875, 1060, 1255, 1282, 1412, 1779, 2211
BspCNI	CTCAG	6 cut(s)	106, 1043, 1078, 1430, 1606, 2516
BspDI	ATCGAT	1 cut(s)	1578
BspEI	TCCGGA	1 cut(s)	1147
BspFNI	CGCG	7 cut(s)	530, 629, 821, 1572, 1823, 2125, 2720
BspLI	GGNNCC	8 cut(s)	589, 778, 1305, 1306, 1518, 1716, 1979, 2643
BspMAI	CTGCAG	1 cut(s)	717
BspPI	GGATC	4 cut(s)	820, 1446, 1566, 1574
BspT104I	TTCGAA	1 cut(s)	938
BspT107I	GGYRCC	2 cut(s)	587, 1516
BspTNI	GGTCTC	2 cut(s)	972, 982
BsrBI	CCGCTC	1 cut(s)	243
BsrDI	GCAATG	6 cut(s)	646, 1342, 1599, 1848, 1851, 2296
BsrFI	RCCGGY	4 cut(s)	441, 1072, 1258, 2662
BsrI	ACTGG	4 cut(s)	702, 777, 1978, 2247
BssAI	RCCGGY	4 cut(s)	441, 1072, 1258, 2662
BssMI	GATC	8 cut(s)	812, 1173, 1438, 1479, 1558, 1575, 1579, 2569
BssNI	GRCGYC	1 cut(s)	885
BssSI	CACGAG	1 cut(s)	634
BssT1I	CCWWGG	3 cut(s)	1052, 1428, 2494
Bst2BI	CACGAG	1 cut(s)	634
Bst2UI	CCWGG	2 cut(s)	2638, 2673
Bst4CI	ACNGT	7 cut(s)	43, 434, 491, 687, 976, 2003, 2542
Bst6I	CTCTTC	3 cut(s)	196, 783, 2418
BstACI	GRCGYC	1 cut(s)	885
BstBI	TTCGAA	1 cut(s)	938
BstC8I	GCNNGC	9 cut(s)	6, 443, 646, 704, 984, 1284, 1414, 1915, 2213
BstDSI	CCRYGG	4 cut(s)	1052, 1428, 2002, 2494
BstF5I	GGATG	5 cut(s)	140, 1843, 2191, 2521, 2713
BstFNI	CGCG	7 cut(s)	530, 629, 821, 1572, 1823, 2125, 2720
BstH2I	RGCGCY	2 cut(s)	1622, 1655
BstHHI	GCGC	2 cut(s)	1621, 1654
BstKTI	GATC	8 cut(s)	815, 1176, 1441, 1482, 1561, 1578, 1582, 2572
BstMAI	GTCTC	8 cut(s)	379, 972, 982, 1355, 1478, 1596, 1892, 2132
BstMBI	GATC	8 cut(s)	812, 1173, 1438, 1479, 1558, 1575, 1579, 2569
BstNI	CCWGG	2 cut(s)	2638, 2673
BstNSI	RCATGY	1 cut(s)	2628
BstSCI	CCNGG	5 cut(s)	1023, 1277, 2181, 2636, 2671
BstSFI	CTRYAG	1 cut(s)	713
BstUI	CGCG	7 cut(s)	530, 629, 821, 1572, 1823, 2125, 2720
BstV1I	GCAGC	8 cut(s)	457, 699, 727, 1677, 1922, 2248, 2251, 2701
BstX2I	RGATCY	1 cut(s)	812
BstXI	CCANNNNNNTGG	1 cut(s)	1053
BstYI	RGATCY	1 cut(s)	812
Bsu15I	ATCGAT	1 cut(s)	1578
BsuRI	GGCC	8 cut(s)	476, 875, 1060, 1255, 1282, 1412, 1779, 2211
BsuTUI	ATCGAT	1 cut(s)	1578
BtgI	CCRYGG	4 cut(s)	1052, 1428, 2002, 2494
BtgZI	GCGATG	4 cut(s)	346, 352, 388, 2022
BtrI	CACGTC	2 cut(s)	358, 2053
BtsCI	GGATG	5 cut(s)	140, 1843, 2191, 2521, 2713
BtsI	GCAGTG	3 cut(s)	254, 387, 1968
BtsIMutI	CAGTG	5 cut(s)	254, 387, 1968, 2254, 2547
Cac8I	GCNNGC	9 cut(s)	6, 443, 646, 704, 984, 1284, 1414, 1915, 2213
CaiI	CAGNNNCTG	2 cut(s)	697, 1546
CfoI	GCGC	2 cut(s)	1621, 1654
Cfr10I	RCCGGY	4 cut(s)	441, 1072, 1258, 2662
Cfr13I	GGNCC	4 cut(s)	1253, 1304, 1410, 1778
ClaI	ATCGAT	1 cut(s)	1578
CseI	GACGC	4 cut(s)	485, 536, 874, 2114
Csp6I	GTAC	2 cut(s)	427, 552
CspAI	ACCGGT	3 cut(s)	1072, 1258, 2662
CspCI	CAANNNNNGTGG	2 cut(s)	817, 852
CviQI	GTAC	2 cut(s)	427, 552
DpnI	GATC	8 cut(s)	814, 1175, 1440, 1481, 1560, 1577, 1581, 2571
DpnII	GATC	8 cut(s)	812, 1173, 1438, 1479, 1558, 1575, 1579, 2569
DrdI	GACNNNNNNGTC	1 cut(s)	489
DseDI	GACNNNNNNGTC	1 cut(s)	489
Eam1104I	CTCTTC	3 cut(s)	196, 783, 2418
EarI	CTCTTC	3 cut(s)	196, 783, 2418
EciI	GGCGGA	5 cut(s)	223, 400, 855, 1179, 1188
Ecl136II	GAGCTC	2 cut(s)	2242, 2502
Eco130I	CCWWGG	3 cut(s)	1052, 1428, 2494
Eco147I	AGGCCT	1 cut(s)	476
Eco24I	GRGCYC	4 cut(s)	704, 1001, 2244, 2504
Eco31I	GGTCTC	2 cut(s)	972, 982
Eco47I	GGWCC	1 cut(s)	1304
Eco53kI	GAGCTC	2 cut(s)	2242, 2502
Eco57I	CTGAAG	3 cut(s)	1154, 1817, 2136
Eco88I	CYCGRG	2 cut(s)	603, 1210
EcoICRI	GAGCTC	2 cut(s)	2242, 2502
EcoO109I	RGGNCCY	1 cut(s)	1304
EcoRI	GAATTC	1 cut(s)	763
EcoRII	CCWGG	2 cut(s)	2636, 2671
EcoT14I	CCWWGG	3 cut(s)	1052, 1428, 2494
EcoT38I	GRGCYC	4 cut(s)	704, 1001, 2244, 2504
ErhI	CCWWGG	3 cut(s)	1052, 1428, 2494
Esp3I	CGTCTC	2 cut(s)	379, 2132
FaqI	GGGAC	6 cut(s)	585, 810, 1006, 1069, 1317, 1696
FauI	CCCGC	5 cut(s)	248, 301, 513, 637, 1309
FblI	GTMKAC	2 cut(s)	45, 2049
FokI	GGATG	5 cut(s)	127, 1850, 2198, 2508, 2720
FriOI	GRGCYC	4 cut(s)	704, 1001, 2244, 2504
FspBI	CTAG	2 cut(s)	2027, 2567
GlaI	GCGC	2 cut(s)	1620, 1653
GsaI	CCCAGC	4 cut(s)	956, 1049, 1784, 2421
GsuI	CTGGAG	2 cut(s)	463, 1925
HaeII	RGCGCY	2 cut(s)	1622, 1655
HaeIII	GGCC	8 cut(s)	476, 875, 1060, 1255, 1282, 1412, 1779, 2211
HgaI	GACGC	4 cut(s)	485, 536, 874, 2114
HhaI	GCGC	2 cut(s)	1621, 1654
Hin1I	GRCGYC	1 cut(s)	885
Hin6I	GCGC	2 cut(s)	1619, 1652
HinP1I	GCGC	2 cut(s)	1619, 1652
HincII	GTYRAC	2 cut(s)	889, 2168
HindII	GTYRAC	2 cut(s)	889, 2168
HindIII	AAGCTT	6 cut(s)	904, 1006, 1772, 1949, 2042, 2204
HphI	GGTGA	5 cut(s)	46, 274, 1331, 2236, 2557
Hpy166II	GTNNAC	6 cut(s)	46, 535, 889, 1501, 2050, 2168
Hpy8I	GTNNAC	6 cut(s)	46, 535, 889, 1501, 2050, 2168
Hpy99I	CGWCG	7 cut(s)	438, 470, 530, 785, 788, 881, 1105
HpyCH4III	ACNGT	7 cut(s)	43, 434, 491, 687, 976, 2003, 2542
HpyCH4IV	ACGT	3 cut(s)	357, 653, 2052
HpySE526I	ACGT	3 cut(s)	357, 653, 2052
Hsp92I	GRCGYC	1 cut(s)	885
HspAI	GCGC	2 cut(s)	1619, 1652
KflI	GGGWCCC	1 cut(s)	1304
Kpn2I	TCCGGA	1 cut(s)	1147
KroI	GCCGGC	1 cut(s)	441
KroNI	GCCGGC	1 cut(s)	443
Kzo9I	GATC	8 cut(s)	812, 1173, 1438, 1479, 1558, 1575, 1579, 2569
LmnI	GCTCC	8 cut(s)	709, 753, 860, 1054, 1231, 1720, 2239, 2499
Lsp1109I	GCAGC	8 cut(s)	457, 699, 727, 1677, 1922, 2248, 2251, 2701
LweI	GCATC	4 cut(s)	932, 1828, 1999, 2369
MaeI	CTAG	2 cut(s)	2027, 2567
MaeII	ACGT	3 cut(s)	357, 653, 2052
MaeIII	GTNAC	4 cut(s)	68, 1627, 2082, 2699
MalI	GATC	8 cut(s)	814, 1175, 1440, 1481, 1560, 1577, 1581, 2571
MbiI	CCGCTC	1 cut(s)	243
MboI	GATC	8 cut(s)	812, 1173, 1438, 1479, 1558, 1575, 1579, 2569
MflI	RGATCY	1 cut(s)	812
MhlI	GDGCHC	6 cut(s)	704, 1001, 1809, 1968, 2244, 2504
MlyI	GAGTC	6 cut(s)	125, 832, 1022, 1357, 1894, 2670
MmeI	TCCRAC	8 cut(s)	152, 489, 549, 804, 882, 1391, 1742, 2569
MroI	TCCGGA	1 cut(s)	1147
MroNI	GCCGGC	1 cut(s)	441
MroXI	GAANNNNTTC	1 cut(s)	2384
MseI	TTAA	8 cut(s)	450, 1623, 1706, 1770, 1827, 2345, 2480, 2598
MslI	CAYNNNNRTG	2 cut(s)	931, 2543
MspA1I	CMGCKG	2 cut(s)	19, 1465
MspR9I	CCNGG	5 cut(s)	1025, 1279, 2183, 2638, 2673
Mva1269I	GAATGC	2 cut(s)	2459, 2653
MvaI	CCWGG	2 cut(s)	2638, 2673
MvnI	CGCG	7 cut(s)	530, 629, 821, 1572, 1823, 2125, 2720
NaeI	GCCGGC	1 cut(s)	443
NciI	CCSGG	3 cut(s)	1025, 1279, 2183
NcoI	CCATGG	3 cut(s)	1052, 1428, 2494
NdeII	GATC	8 cut(s)	812, 1173, 1438, 1479, 1558, 1575, 1579, 2569
NgoMIV	GCCGGC	1 cut(s)	441
NlaIV	GGNNCC	8 cut(s)	589, 778, 1305, 1306, 1518, 1716, 1979, 2643
NmeAIII	GCCGAG	2 cut(s)	564, 1810
NspI	RCATGY	1 cut(s)	2628
NspV	TTCGAA	1 cut(s)	938
OliI	CACNNNNGTG	1 cut(s)	2543
PasI	CCCWGGG	1 cut(s)	2637
PceI	AGGCCT	1 cut(s)	476
PcsI	WCGNNNNNNNCGW	1 cut(s)	369
PctI	GAATGC	2 cut(s)	2459, 2653
PdiI	GCCGGC	1 cut(s)	443
PdmI	GAANNNNTTC	1 cut(s)	2384
PfeI	GAWTC	9 cut(s)	1138, 1543, 1760, 1865, 2140, 2146, 2273, 2390, 2437
PflFI	GACNNNGTC	1 cut(s)	1529
PflMI	CCANNNNNTGG	2 cut(s)	1429, 1434
PfoI	TCCNGGA	2 cut(s)	2181, 2671
PinAI	ACCGGT	3 cut(s)	1072, 1258, 2662
PleI	GAGTC	6 cut(s)	124, 831, 1022, 1357, 1894, 2670
PpsI	GAGTC	6 cut(s)	124, 831, 1022, 1357, 1894, 2670
PpuMI	RGGWCCY	1 cut(s)	1304
PshBI	ATTAAT	1 cut(s)	2345
Psp124BI	GAGCTC	2 cut(s)	2244, 2504
Psp5II	RGGWCCY	1 cut(s)	1304
Psp6I	CCWGG	2 cut(s)	2636, 2671
PspFI	CCCAGC	4 cut(s)	952, 1045, 1780, 2417
PspGI	CCWGG	2 cut(s)	2636, 2671
PspN4I	GGNNCC	8 cut(s)	589, 778, 1305, 1306, 1518, 1716, 1979, 2643
PspPI	GGNCC	4 cut(s)	1253, 1304, 1410, 1778
PspPPI	RGGWCCY	1 cut(s)	1304
PstI	CTGCAG	1 cut(s)	717
PstNI	CAGNNNCTG	2 cut(s)	697, 1546
PsuI	RGATCY	1 cut(s)	812
PsyI	GACNNNGTC	1 cut(s)	1529
PvuII	CAGCTG	1 cut(s)	1465
RsaI	GTAC	2 cut(s)	428, 553
RsaNI	GTAC	2 cut(s)	427, 552
RseI	CAYNNNNRTG	2 cut(s)	931, 2543
SacI	GAGCTC	2 cut(s)	2244, 2504
SaqAI	TTAA	8 cut(s)	450, 1623, 1706, 1770, 1827, 2345, 2480, 2598
Sau3AI	GATC	8 cut(s)	812, 1173, 1438, 1479, 1558, 1575, 1579, 2569
Sau96I	GGNCC	4 cut(s)	1253, 1304, 1410, 1778
SchI	GAGTC	6 cut(s)	125, 832, 1022, 1357, 1894, 2670
ScrFI	CCNGG	5 cut(s)	1025, 1279, 2183, 2638, 2673
SduI	GDGCHC	6 cut(s)	704, 1001, 1809, 1968, 2244, 2504
SfaNI	GCATC	4 cut(s)	932, 1828, 1999, 2369
SfcI	CTRYAG	1 cut(s)	713
SfuI	TTCGAA	1 cut(s)	938
SinI	GGWCC	1 cut(s)	1304
SmiMI	CAYNNNNRTG	2 cut(s)	931, 2543
SmlI	CTYRAG	4 cut(s)	1108, 1946, 2039, 2445
SmoI	CTYRAG	4 cut(s)	1108, 1946, 2039, 2445
SseBI	AGGCCT	1 cut(s)	476
SspI	AATATT	1 cut(s)	2489
SspMI	CTAG	2 cut(s)	2027, 2567
SstI	GAGCTC	2 cut(s)	2244, 2504
StuI	AGGCCT	1 cut(s)	476
StyD4I	CCNGG	5 cut(s)	1023, 1277, 2181, 2636, 2671
StyI	CCWWGG	3 cut(s)	1052, 1428, 2494
TaaI	ACNGT	7 cut(s)	43, 434, 491, 687, 976, 2003, 2542
TaiI	ACGT	3 cut(s)	360, 656, 2055
TatI	WGTACW	1 cut(s)	426
TauI	GCSGC	6 cut(s)	416, 419, 629, 721, 1060, 1204
TfiI	GAWTC	9 cut(s)	1138, 1543, 1760, 1865, 2140, 2146, 2273, 2390, 2437
Tru1I	TTAA	8 cut(s)	450, 1623, 1706, 1770, 1827, 2345, 2480, 2598
Tru9I	TTAA	8 cut(s)	450, 1623, 1706, 1770, 1827, 2345, 2480, 2598
TscAI	CASTG	5 cut(s)	261, 394, 1968, 2254, 2547
TseI	GCWGC	8 cut(s)	445, 712, 715, 1690, 1910, 2261, 2264, 2689
TspDTI	ATGAA	8 cut(s)	385, 1653, 1773, 2000, 2153, 2307, 2616, 2727
TspGWI	ACGGA	2 cut(s)	766, 1750
TspRI	CASTG	5 cut(s)	261, 394, 1968, 2254, 2547
Tth111I	GACNNNGTC	1 cut(s)	1529
Van91I	CCANNNNNTGG	2 cut(s)	1429, 1434
VpaK11BI	GGWCC	1 cut(s)	1304
VspI	ATTAAT	1 cut(s)	2345
XapI	RAATTY	4 cut(s)	763, 1790, 2381, 2532
XbaI	TCTAGA	1 cut(s)	2566
XceI	RCATGY	1 cut(s)	2628
XmiI	GTMKAC	2 cut(s)	45, 2049
XmnI	GAANNNNTTC	1 cut(s)	2384
XspI	CTAG	2 cut(s)	2027, 2567

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.