Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0011760

Physical Location & Seq

Genomic Coordinates Reverse (-)

430098 .. 433933

Gene Structure 3836 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000015652

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2727 bp

ATGGCTCCTCTCACTCTGATCATCTTCCTCCTCCGCTTCACCACCGCCACTTGTCACCGCCACCTCCTCCACCAGCCCTTCTTCCCAGTCGAATCAAACCCTCCAACCCAACCACCATCGTCCTCTCCCCACCCCTCTCAAATTCCCAAAATGCCCTTCTCCTCCGCCTCATCCTCCTCCTCAACACCACCTGATAATAACAAACCGTTCTTTCCCTCCATCAAACCCACACCGCCTCCTCCCTCCCCAACAGCCCTCACCTCCTTCCCCGCCAACATTTCCTCCATCCTTTTCCCCCAACCCTCTTCCTCCTCCCCCCACCGCCACGTCATCGCCATCGGCGTCTCGGTCTCCGTCACGGCTGCACTCATCATCGCCTTCGCCGCTGCCTTTTTACTCTACCGCCGGCGCCGGAAGCTCGACGCGACCTCGGCCGACGACAAGGCCTCCAGAACCGACAGCCTCCGCCTGTTCCCGCCCAACACCGTCACCTCCGACGGAGTTCACAAGCAGCGTACCACACCCAACAGAACCACCGAGAACTTCCTCTACCTCGGCACATTATCCAACGCTCAAGGAAACGACGAACAAAGCGTCGCCACCACGAGCAATGCCAGCTCGAAGTTCGGGGTTTCAAGCCGGTCTGGCTCGCCGTATCAGAAGCTAGGTTCGCCGGAGCTGACGCCGCTTCCTCCACTTCCCAAGGACAATTTCATACGGAGCTACAAGCATGAACTGGGTTCCGACGATGATGAAGGGGAAGACGAGTTCTTTTCTCCGAGAGGTTCTTCTGCGAGTCCGAAAAAGGTCGCCAGAGCAAGCTCAGATAGAGTGCTCAAGGCTGTAGAAGGCGACAACTTTGGAAGCAGGAGCTTCAATTCAAGAACTGCATCATACCCATGTTCAAAATCTGCTTCTCCAGTAAGTTCTGTGTCCAACACTGTCTCACCACAGTTGAACTTTAGTCCCACAAGCTTGAAATCGAACTCGCCGGATTCTGTGATGAATTTCATGGCACCATCTCGGCCTGCTCCGATTCCGGTGGCAGTGTCCCGGTCATTGTCTTCGTCGTCTACTTTATCAGAGAGAGTTTCAGGGAATGTGCAGAGTTCCCCTGACTTTTTGACGCAGATGAAGCAGTCTCTAGTGAGAAACAATAGTGCTCCCAAGCGGTTTGTTCCAATAAGACTGCCTCCGCCTCCTCCTCCACCGCCACCGCCCAGGTATTGGGAAGCTCCGGTGGCGAATGCAGGTCCACCTGTTCATGTCACGCCTTCCAGGGCTCGTGTGTTTGAGAATAATATGGGACCAGTTTTGGCTGGTGAACCATCACAGAAGAATGACATTGAGGAAAAAAGCGAGGAAACTCCTAGGCCAAAGTTGAAGCCTTTACATTGGGACAAAGTTAGGGCCAGCTCAGACCGCGCTATGGTGTGGGATCAGTTAAAATCAAGCTCCTTTCAGTTGAATGAGGAAATGATTGAGACACTTTTTGTGGTAAACAATTCAAATTTGACACCAAAAGAAAATGCTCGGCGACAGATTCTGCCCTCTTTGAATCAGGAGAACCGTGTGCTGGATCCAAAGAAGTCTCAGAATATTGCAATTTTGTTAAGAGCGCTCAATGTTACTATTGATGAAGTGTGTGAAGCGCTCTTGGAAGGTAATTCGGATTCTCTCGGAACAGAACTTCTTGAAAGTTTATTAAAGATGGCTCCAACCAAAGAGGAAGAATGGAAGCTAAAAGAGTTCAAAGATGAATCACCATTTAAGCTTGGCCCAGCTGAGAAATTTCTGAAGGCAGTGCTTGATGTACCTTATGCATTTAAGAGGGTGGATGCAATGCTGTACATTGCCAATTTTGATTCAGAAGTAGACTATCTTAAAAGGTCTTTTGAGACATTGGAGGCAGCTTGTGAAGAATTGAAGAACAGCAGAATGTTCTTTAAGCTTCTTGAAGCAGTGCTCAAAACTGGGAACCGCATGAATGTCGGCACCAACCGTGGCGATGCTCATGCCTTCAAGCTCGACACACTCCTGAAGCTGGTAGATGTTAAGGGCACTGATGGAAAAACTACTCTGTTGCATTTTGTAGTACAGGAGATCATCAGAGCTGAAGGTTCTCGTCTTTCGGGCATGAATCAGGATGAAACGGCTGAGAGCAGTCAACAATCTTCTTTCCGGGATGATGTGGAGTTTAGGAAGCGTGGTCTGCAAGTTGTTTCAGGATTGAGTGGAGAGCTCATCAATGTAAAGAAAGCTGCTGGTATGGATTCAGATGTACTGAGCAACGAAGTAGCAAAGATTGCTGGGGGAATTACAAAAATTGAAGAAGTTGTAAAATTAATTGAAGGAAGTGCATTGAAGGAGAGTAGCCAGAAGTTCTTAGACTCCATGAATGGGTTCTTGAAGAAGGCAAGTGAAGAGATTGTGATGATTCAAGCTCGAGAGAAACTTGCATTCTGTATGGTGAAGGAAATAACAGAATATTTCCATGGGAACTCAGCTAAAGAAGAAGCTCATCCTTTCCGGATTTTCACGGTGGTGAGAGATTTCCTTTCAATTCTAGATCAGGCATGCAAGGTAGTCGGCAAAGTTAATGAAAGAACTATAGTTGGGTCTGCCCGTCAATTTCCAATTGTCATGAATCCAACCGTTCCACCAGTTTTTCCTGGACCAAGTACAAAGCAGCATTATGGTTCCTCGGATGATGAAACCTTGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009987 GO:0010638 GO:0010639 GO:0016020 GO:0016043 GO:0030029 GO:0030036 GO:0030312 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0030838 GO:0031333 GO:0031334 GO:0032271 GO:0032272 GO:0032273 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0043242 GO:0043244 GO:0043254 GO:0044087 GO:0044089 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051015 GO:0051016 GO:0051128 GO:0051129 GO:0051130 GO:0051493 GO:0051494 GO:0051495 GO:0051693 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0097435 GO:0110053 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04320 map04320

Pfam Domains

Protein Families

FH2

Protein Analysis

909

Amino Acids

99.69

Weight (kDa)

8.76

Isoelectric Point (pI)

57.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	452 - 856	4.2e-115	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011760)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43800
fragaria_vesca	FvH4_1g00460
malus_domestica	MD02G1003800.v1.1 MD15G1147100.v1.1
prunus_persica	Prupe.7G267500_v2.0.a1
pyrus_communis	pycom02g00230 pycom15g13200
rosa_chinensis	RchiOBHm_Chr2g0084971
rosa_laevigata	RLG00000015652
rosa_multiflora	Rmu_co8206718.1_g000001 Rmu_sc0017508.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155670
rosa_rugosa	Rorug01G0457400
rosa_samantha	Rh2AG005000 Rh2BG006200 Rh2BG300200 Rh2CG006800 Rh2DG006600
rosa_wichuraiana	Rw2G000500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1241
AccB1I	GGYRCC	3 cut(s)	408, 1015, 1994
AccB7I	CCANNNNNTGG	1 cut(s)	1227
AccI	GTMKAC	2 cut(s)	1073, 1875
AccII	CGCG	2 cut(s)	425, 1425
AccIII	TCCGGA	1 cut(s)	2529
AclWI	GGATC	3 cut(s)	1446, 1574, 1587
AcoI	YGGCCR	1 cut(s)	432
AcsI	RAATTY	4 cut(s)	141, 1006, 1510, 1790
AcuI	CTGAAG	3 cut(s)	1817, 2060, 2136
AcyI	GRCGYC	3 cut(s)	342, 409, 683
AfaI	GTAC	6 cut(s)	517, 1815, 1850, 2097, 2283, 2683
AfeI	AGCGCT	2 cut(s)	1620, 1653
AfiI	CCNNNNNNNGG	5 cut(s)	1227, 1429, 1576, 2044, 2400
AjiI	CACGTC	1 cut(s)	328
AjnI	CCWGG	3 cut(s)	1220, 1277, 2671
AjuI	GAANNNNNNNTTGG	4 cut(s)	140, 172, 695, 727
AleI	CACNNNNGTG	1 cut(s)	2543
AloI	GAACNNNNNNTCC	2 cut(s)	752, 784
Alw21I	GWGCWC	4 cut(s)	837, 1165, 1968, 2244
Alw26I	GTCTC	7 cut(s)	349, 355, 949, 1146, 1478, 1596, 1892
AlwI	GGATC	3 cut(s)	1446, 1574, 1587
AlwNI	CAGNNNCTG	1 cut(s)	1546
Ama87I	CYCGRG	1 cut(s)	2445
Aor13HI	TCCGGA	1 cut(s)	2529
Aor51HI	AGCGCT	2 cut(s)	1620, 1653
AoxI	GGCC	6 cut(s)	432, 444, 1025, 1373, 1410, 1777
ApeKI	GCWGC	6 cut(s)	362, 386, 511, 1910, 2261, 2689
ApoI	RAATTY	4 cut(s)	141, 1006, 1510, 1790
ArsI	GACNNNNNNTTYG	2 cut(s)	579, 611
AseI	ATTAAT	1 cut(s)	2345
Asp700I	GAANNNNTTC	1 cut(s)	2402
AspA2I	CCTAGG	1 cut(s)	1370
AspLEI	GCGC	4 cut(s)	411, 1427, 1621, 1654
AspS9I	GGNCC	5 cut(s)	1253, 1307, 1410, 1778, 2675
AsuC2I	CCSGG	2 cut(s)	1054, 2183
AsuHPI	GGTGA	9 cut(s)	31, 47, 250, 481, 939, 1334, 1755, 2482, 2557
AvaI	CYCGRG	1 cut(s)	2445
AvaII	GGWCC	3 cut(s)	1253, 1307, 2675
AvrII	CCTAGG	1 cut(s)	1370
BaeGI	GKGCMC	1 cut(s)	2063
BamHI	GGATCC	1 cut(s)	1579
BanI	GGYRCC	3 cut(s)	408, 1015, 1994
BanII	GRGCYC	2 cut(s)	1285, 2244
BauI	CACGAG	2 cut(s)	604, 1284
BbsI	GAAGAC	2 cut(s)	768, 1056
Bbv12I	GWGCWC	4 cut(s)	837, 1165, 1968, 2244
BbvI	GCAGC	6 cut(s)	349, 373, 523, 1922, 2248, 2701
BccI	CCATC	8 cut(s)	124, 227, 293, 344, 1027, 1336, 1705, 2060
BceAI	ACGGC	3 cut(s)	375, 637, 2169
BcgI	CGANNNNNNTGC	6 cut(s)	1972, 1997, 2006, 2031, 2569, 2603
BciT130I	CCWGG	3 cut(s)	1222, 1279, 2673
BclI	TGATCA	1 cut(s)	18
BcnI	CCSGG	2 cut(s)	1054, 2183
BcoDI	GTCTC	7 cut(s)	349, 355, 949, 1146, 1478, 1596, 1892
BfaI	CTAG	4 cut(s)	665, 1145, 1371, 2567
BfmI	CTRYAG	2 cut(s)	843, 2610
BfoI	RGCGCY	3 cut(s)	412, 1622, 1655
BfuAI	ACCTGC	1 cut(s)	1241
BglI	GCCNNNNNGGC	1 cut(s)	645
BisI	GCNGC	8 cut(s)	363, 384, 387, 512, 686, 1911, 2262, 2690
BlnI	CCTAGG	1 cut(s)	1370
BlsI	GCNGC	8 cut(s)	364, 385, 388, 513, 687, 1912, 2263, 2691
Bme1390I	CCNGG	5 cut(s)	1054, 1222, 1279, 2183, 2673
Bme18I	GGWCC	3 cut(s)	1253, 1307, 2675
BmeT110I	CYCGRG	1 cut(s)	2445
BmgBI	CACGTC	1 cut(s)	328
BmgT120I	GGNCC	5 cut(s)	1253, 1307, 1410, 1778, 2675
BmrFI	CCNGG	5 cut(s)	1054, 1222, 1279, 2183, 2673
BmrI	ACTGGG	3 cut(s)	80, 746, 1983
BmsI	GCATC	3 cut(s)	899, 1828, 1999
BmuI	ACTGGG	3 cut(s)	80, 746, 1983
BpiI	GAAGAC	2 cut(s)	768, 1056
BpmI	CTGGAG	2 cut(s)	433, 903
BpuEI	CTTGAG	2 cut(s)	558, 821
BpuMI	CCSGG	2 cut(s)	1054, 2183
BsaHI	GRCGYC	3 cut(s)	342, 409, 683
BsaI	GGTCTC	1 cut(s)	355
BsaJI	CCNNGG	9 cut(s)	429, 553, 702, 1220, 1278, 1370, 2002, 2494, 2703
BsaWI	WCCGGW	3 cut(s)	1039, 1237, 2529
BsaXI	ACNNNNNCTCC	6 cut(s)	220, 250, 266, 296, 431, 461
Bsc4I	CCNNNNNNNGG	5 cut(s)	1227, 1429, 1576, 2044, 2400
Bse118I	RCCGGY	2 cut(s)	405, 639
Bse1I	ACTGG	6 cut(s)	86, 741, 920, 1310, 1978, 2663
Bse3DI	GCAATG	3 cut(s)	616, 1848, 1851
BseAI	TCCGGA	1 cut(s)	2529
BseBI	CCWGG	3 cut(s)	1222, 1279, 2673
BseDI	CCNNGG	9 cut(s)	429, 553, 702, 1220, 1278, 1370, 2002, 2494, 2703
BseGI	GGATG	7 cut(s)	170, 285, 1843, 2152, 2191, 2521, 2713
BseLI	CCNNNNNNNGG	5 cut(s)	1227, 1429, 1576, 2044, 2400
BseMI	GCAATG	3 cut(s)	616, 1848, 1851
BseMII	CTCAG	7 cut(s)	837, 1431, 1607, 1776, 2148, 2276, 2517
BseNI	ACTGG	6 cut(s)	86, 741, 920, 1310, 1978, 2663
BseRI	GAGGAG	9 cut(s)	20, 56, 151, 166, 169, 228, 301, 1191, 1194
BseSI	GKGCMC	1 cut(s)	2063
BseX3I	CGGCCG	1 cut(s)	432
BseXI	GCAGC	6 cut(s)	349, 373, 523, 1922, 2248, 2701
BseYI	CCCAGC	2 cut(s)	1780, 2309
BsgI	GTGCAG	2 cut(s)	348, 1124
Bsh1236I	CGCG	2 cut(s)	425, 1425
Bsh1285I	CGRYCG	1 cut(s)	435
BshFI	GGCC	6 cut(s)	434, 446, 1027, 1375, 1412, 1779
BshNI	GGYRCC	3 cut(s)	408, 1015, 1994
BsiEI	CGRYCG	1 cut(s)	435
BsiHKAI	GWGCWC	4 cut(s)	837, 1165, 1968, 2244
BsiHKCI	CYCGRG	1 cut(s)	2445
BslFI	GGGAC	4 cut(s)	951, 1036, 1320, 1412
BslI	CCNNNNNNNGG	5 cut(s)	1227, 1429, 1576, 2044, 2400
BsmAI	GTCTC	7 cut(s)	349, 355, 949, 1146, 1478, 1596, 1892
BsmBI	CGTCTC	1 cut(s)	349
BsmFI	GGGAC	4 cut(s)	951, 1036, 1320, 1412
BsmI	GAATGC	2 cut(s)	1252, 2459
BsnI	GGCC	6 cut(s)	434, 446, 1027, 1375, 1412, 1779
Bso31I	GGTCTC	1 cut(s)	355
BsoBI	CYCGRG	1 cut(s)	2445
Bsp1286I	GDGCHC	6 cut(s)	837, 1165, 1285, 1968, 2063, 2244
Bsp13I	TCCGGA	1 cut(s)	2529
Bsp1407I	TGTACA	1 cut(s)	1848
Bsp143I	GATC	5 cut(s)	18, 1438, 1579, 2103, 2569
Bsp19I	CCATGG	1 cut(s)	2494
BspANI	GGCC	6 cut(s)	434, 446, 1027, 1375, 1412, 1779
BspCNI	CTCAG	7 cut(s)	836, 1430, 1606, 1777, 2149, 2277, 2516
BspEI	TCCGGA	1 cut(s)	2529
BspFNI	CGCG	2 cut(s)	425, 1425
BspHI	TCATGA	1 cut(s)	2643
BspMI	ACCTGC	1 cut(s)	1241
BspPI	GGATC	3 cut(s)	1446, 1574, 1587
BspT107I	GGYRCC	3 cut(s)	408, 1015, 1994
BspTNI	GGTCTC	1 cut(s)	355
BsrDI	GCAATG	3 cut(s)	616, 1848, 1851
BsrFI	RCCGGY	2 cut(s)	405, 639
BsrGI	TGTACA	1 cut(s)	1848
BsrI	ACTGG	6 cut(s)	86, 741, 920, 1310, 1978, 2663
BssAI	RCCGGY	2 cut(s)	405, 639
BssECI	CCNNGG	9 cut(s)	429, 553, 702, 1220, 1278, 1370, 2002, 2494, 2703
BssMI	GATC	5 cut(s)	18, 1438, 1579, 2103, 2569
BssNI	GRCGYC	3 cut(s)	342, 409, 683
BssSI	CACGAG	2 cut(s)	604, 1284
BssT1I	CCWWGG	3 cut(s)	702, 1370, 2494
Bst2BI	CACGAG	2 cut(s)	604, 1284
Bst2UI	CCWGG	3 cut(s)	1222, 1279, 2673
Bst4CI	ACNGT	8 cut(s)	207, 487, 943, 954, 1571, 2003, 2542, 2656
Bst6I	CTCTTC	2 cut(s)	310, 2418
BstACI	GRCGYC	3 cut(s)	342, 409, 683
BstAPI	GCANNNNNTGC	1 cut(s)	2306
BstAUI	TGTACA	1 cut(s)	1848
BstC8I	GCNNGC	7 cut(s)	407, 616, 650, 820, 1029, 1414, 2578
BstDEI	CTNAG	8 cut(s)	823, 1417, 1593, 1785, 2157, 2285, 2386, 2503
BstDSI	CCRYGG	2 cut(s)	2002, 2494
BstF5I	GGATG	7 cut(s)	170, 285, 1843, 2152, 2191, 2521, 2713
BstFNI	CGCG	2 cut(s)	425, 1425
BstH2I	RGCGCY	3 cut(s)	412, 1622, 1655
BstHHI	GCGC	4 cut(s)	411, 1427, 1621, 1654
BstKTI	GATC	5 cut(s)	21, 1441, 1582, 2106, 2572
BstMAI	GTCTC	7 cut(s)	349, 355, 949, 1146, 1478, 1596, 1892
BstMBI	GATC	5 cut(s)	18, 1438, 1579, 2103, 2569
BstMCI	CGRYCG	1 cut(s)	435
BstNI	CCWGG	3 cut(s)	1222, 1279, 2673
BstNSI	RCATGY	1 cut(s)	2580
BstSCI	CCNGG	5 cut(s)	1052, 1220, 1277, 2181, 2671
BstSFI	CTRYAG	2 cut(s)	843, 2610
BstSLI	GKGCMC	1 cut(s)	2063
BstUI	CGCG	2 cut(s)	425, 1425
BstV1I	GCAGC	6 cut(s)	349, 373, 523, 1922, 2248, 2701
BstV2I	GAAGAC	2 cut(s)	768, 1056
BstX2I	RGATCY	1 cut(s)	1579
BstYI	RGATCY	1 cut(s)	1579
BstZI	CGGCCG	1 cut(s)	432
BsuRI	GGCC	6 cut(s)	434, 446, 1027, 1375, 1412, 1779
BtgI	CCRYGG	2 cut(s)	2002, 2494
BtgZI	GCGATG	3 cut(s)	316, 358, 2022
BtrI	CACGTC	1 cut(s)	328
BtsCI	GGATG	7 cut(s)	170, 285, 1843, 2152, 2191, 2521, 2713
BtsI	GCAGTG	3 cut(s)	1053, 1809, 1968
BtsIMutI	CAGTG	5 cut(s)	939, 1053, 1809, 1968, 2061
BveI	ACCTGC	1 cut(s)	1241
Cac8I	GCNNGC	7 cut(s)	407, 616, 650, 820, 1029, 1414, 2578
CaiI	CAGNNNCTG	1 cut(s)	1546
CciI	TCATGA	1 cut(s)	2643
CfoI	GCGC	4 cut(s)	411, 1427, 1621, 1654
Cfr10I	RCCGGY	2 cut(s)	405, 639
Cfr13I	GGNCC	5 cut(s)	1253, 1307, 1410, 1778, 2675
CseI	GACGC	5 cut(s)	331, 431, 583, 691, 1135
Csp6I	GTAC	6 cut(s)	516, 1814, 1849, 2096, 2282, 2682
CviQI	GTAC	6 cut(s)	516, 1814, 1849, 2096, 2282, 2682
DdeI	CTNAG	8 cut(s)	823, 1417, 1593, 1785, 2157, 2285, 2386, 2503
DinI	GGCGCC	1 cut(s)	410
DpnI	GATC	5 cut(s)	20, 1440, 1581, 2105, 2571
DpnII	GATC	5 cut(s)	18, 1438, 1579, 2103, 2569
EaeI	YGGCCR	1 cut(s)	432
EagI	CGGCCG	1 cut(s)	432
Eam1104I	CTCTTC	2 cut(s)	310, 2418
EarI	CTCTTC	2 cut(s)	310, 2418
EciI	GGCGGA	3 cut(s)	154, 455, 1185
Ecl136II	GAGCTC	1 cut(s)	2242
EclXI	CGGCCG	1 cut(s)	432
Eco130I	CCWWGG	3 cut(s)	702, 1370, 2494
Eco147I	AGGCCT	1 cut(s)	446
Eco24I	GRGCYC	2 cut(s)	1285, 2244
Eco31I	GGTCTC	1 cut(s)	355
Eco47I	GGWCC	3 cut(s)	1253, 1307, 2675
Eco47III	AGCGCT	2 cut(s)	1620, 1653
Eco52I	CGGCCG	1 cut(s)	432
Eco53kI	GAGCTC	1 cut(s)	2242
Eco57I	CTGAAG	3 cut(s)	1817, 2060, 2136
Eco88I	CYCGRG	1 cut(s)	2445
EcoICRI	GAGCTC	1 cut(s)	2242
EcoRII	CCWGG	3 cut(s)	1220, 1277, 2671
EcoT14I	CCWWGG	3 cut(s)	702, 1370, 2494
EcoT22I	ATGCAT	1 cut(s)	1825
EcoT38I	GRGCYC	2 cut(s)	1285, 2244
EgeI	GGCGCC	1 cut(s)	410
EheI	GGCGCC	1 cut(s)	410
ErhI	CCWWGG	3 cut(s)	702, 1370, 2494
Esp3I	CGTCTC	1 cut(s)	349
FaqI	GGGAC	4 cut(s)	951, 1036, 1320, 1412
FauI	CCCGC	2 cut(s)	277, 483
FbaI	TGATCA	1 cut(s)	18
FblI	GTMKAC	2 cut(s)	1073, 1875
Fnu4HI	GCNGC	8 cut(s)	363, 384, 387, 512, 686, 1911, 2262, 2690
FokI	GGATG	7 cut(s)	157, 272, 1850, 2159, 2198, 2508, 2720
FriOI	GRGCYC	2 cut(s)	1285, 2244
Fsp4HI	GCNGC	8 cut(s)	363, 384, 387, 512, 686, 1911, 2262, 2690
FspBI	CTAG	4 cut(s)	665, 1145, 1371, 2567
GlaI	GCGC	4 cut(s)	410, 1426, 1620, 1653
GluI	GCNGC	8 cut(s)	363, 384, 387, 512, 686, 1911, 2262, 2690
GsaI	CCCAGC	2 cut(s)	1784, 2313
GsuI	CTGGAG	2 cut(s)	433, 903
HaeII	RGCGCY	3 cut(s)	412, 1622, 1655
HaeIII	GGCC	6 cut(s)	434, 446, 1027, 1375, 1412, 1779
HgaI	GACGC	5 cut(s)	331, 431, 583, 691, 1135
HhaI	GCGC	4 cut(s)	411, 1427, 1621, 1654
Hin1I	GRCGYC	3 cut(s)	342, 409, 683
Hin6I	GCGC	4 cut(s)	409, 1425, 1619, 1652
HinP1I	GCGC	4 cut(s)	409, 1425, 1619, 1652
HincII	GTYRAC	1 cut(s)	2168
HindII	GTYRAC	1 cut(s)	2168
HindIII	AAGCTT	3 cut(s)	973, 1772, 1949
HphI	GGTGA	9 cut(s)	31, 47, 250, 481, 939, 1334, 1755, 2482, 2557
Hpy166II	GTNNAC	7 cut(s)	505, 1074, 1256, 1325, 1501, 1876, 2168
Hpy8I	GTNNAC	7 cut(s)	505, 1074, 1256, 1325, 1501, 1876, 2168
Hpy99I	CGWCG	7 cut(s)	425, 440, 500, 587, 599, 749, 1072
HpyCH4III	ACNGT	8 cut(s)	207, 487, 943, 954, 1571, 2003, 2542, 2656
HpyCH4IV	ACGT	1 cut(s)	327
HpyF3I	CTNAG	8 cut(s)	823, 1417, 1593, 1785, 2157, 2285, 2386, 2503
HpySE526I	ACGT	1 cut(s)	327
Hsp92I	GRCGYC	3 cut(s)	342, 409, 683
HspAI	GCGC	4 cut(s)	409, 1425, 1619, 1652
KasI	GGCGCC	1 cut(s)	408
Kpn2I	TCCGGA	1 cut(s)	2529
KroI	GCCGGC	1 cut(s)	405
KroNI	GCCGGC	1 cut(s)	407
Ksp22I	TGATCA	1 cut(s)	18
Kzo9I	GATC	5 cut(s)	18, 1438, 1579, 2103, 2569
LmnI	GCTCC	9 cut(s)	10, 676, 720, 870, 1036, 1168, 1240, 1460, 1720
Lsp1109I	GCAGC	6 cut(s)	349, 373, 523, 1922, 2248, 2701
LweI	GCATC	3 cut(s)	899, 1828, 1999
MaeI	CTAG	4 cut(s)	665, 1145, 1371, 2567
MaeII	ACGT	1 cut(s)	327
MaeIII	GTNAC	5 cut(s)	53, 355, 487, 1267, 1627
MalI	GATC	5 cut(s)	20, 1440, 1581, 2105, 2571
MboI	GATC	5 cut(s)	18, 1438, 1579, 2103, 2569
MfeI	CAATTG	1 cut(s)	2637
MflI	RGATCY	1 cut(s)	1579
MhlI	GDGCHC	6 cut(s)	837, 1165, 1285, 1968, 2063, 2244
Mly113I	GGCGCC	1 cut(s)	409
MlyI	GAGTC	2 cut(s)	805, 2384
MmeI	TCCRAC	7 cut(s)	128, 519, 591, 768, 960, 1742, 2675
Mph1103I	ATGCAT	1 cut(s)	1825
MreI	CGCCGGCG	1 cut(s)	405
MroI	TCCGGA	1 cut(s)	2529
MroNI	GCCGGC	1 cut(s)	405
MroXI	GAANNNNTTC	1 cut(s)	2402
MslI	CAYNNNNRTG	2 cut(s)	898, 2543
MspA1I	CMGCKG	2 cut(s)	386, 1784
MspR9I	CCNGG	5 cut(s)	1054, 1222, 1279, 2183, 2673
MunI	CAATTG	1 cut(s)	2637
Mva1269I	GAATGC	2 cut(s)	1252, 2459
MvaI	CCWGG	3 cut(s)	1222, 1279, 2673
MvnI	CGCG	2 cut(s)	425, 1425
NaeI	GCCGGC	1 cut(s)	407
NarI	GGCGCC	1 cut(s)	409
NciI	CCSGG	2 cut(s)	1054, 2183
NcoI	CCATGG	1 cut(s)	2494
NdeII	GATC	5 cut(s)	18, 1438, 1579, 2103, 2569
NgoMIV	GCCGGC	1 cut(s)	405
NmeAIII	GCCGAG	4 cut(s)	410, 534, 1003, 1513
NmuCI	GTSAC	4 cut(s)	53, 355, 487, 1267
NsiI	ATGCAT	1 cut(s)	1825
NspI	RCATGY	1 cut(s)	2580
OliI	CACNNNNGTG	1 cut(s)	2543
PaeI	GCATGC	1 cut(s)	2580
PaeR7I	CTCGAG	1 cut(s)	2445
PagI	TCATGA	1 cut(s)	2643
PceI	AGGCCT	1 cut(s)	446
PcsI	WCGNNNNNNNCGW	2 cut(s)	339, 591
PctI	GAATGC	2 cut(s)	1252, 2459
PdiI	GCCGGC	1 cut(s)	407
PdmI	GAANNNNTTC	1 cut(s)	2402
PflMI	CCANNNNNTGG	1 cut(s)	1227
PfoI	TCCNGGA	2 cut(s)	2181, 2671
PkrI	GCNGC	8 cut(s)	364, 385, 388, 513, 687, 1912, 2263, 2691
PleI	GAGTC	2 cut(s)	804, 2384
PluTI	GGCGCC	1 cut(s)	412
PpsI	GAGTC	2 cut(s)	804, 2384
PshBI	ATTAAT	1 cut(s)	2345
Psp124BI	GAGCTC	1 cut(s)	2244
Psp6I	CCWGG	3 cut(s)	1220, 1277, 2671
PspFI	CCCAGC	2 cut(s)	1780, 2309
PspGI	CCWGG	3 cut(s)	1220, 1277, 2671
PspPI	GGNCC	5 cut(s)	1253, 1307, 1410, 1778, 2675
PsrI	GAACNNNNNNTAC	2 cut(s)	533, 565
PstNI	CAGNNNCTG	1 cut(s)	1546
PsuI	RGATCY	1 cut(s)	1579
PvuII	CAGCTG	1 cut(s)	1784
RsaI	GTAC	6 cut(s)	517, 1815, 1850, 2097, 2283, 2683
RsaNI	GTAC	6 cut(s)	516, 1814, 1849, 2096, 2282, 2682
RseI	CAYNNNNRTG	2 cut(s)	898, 2543
SacI	GAGCTC	1 cut(s)	2244
SatI	GCNGC	8 cut(s)	363, 384, 387, 512, 686, 1911, 2262, 2690
Sau3AI	GATC	5 cut(s)	18, 1438, 1579, 2103, 2569
Sau96I	GGNCC	5 cut(s)	1253, 1307, 1410, 1778, 2675
SchI	GAGTC	2 cut(s)	805, 2384
ScrFI	CCNGG	5 cut(s)	1054, 1222, 1279, 2183, 2673
SduI	GDGCHC	6 cut(s)	837, 1165, 1285, 1968, 2063, 2244
SfaNI	GCATC	3 cut(s)	899, 1828, 1999
SfcI	CTRYAG	2 cut(s)	843, 2610
SfoI	GGCGCC	1 cut(s)	410
Sfr274I	CTCGAG	1 cut(s)	2445
SgrAI	CRCCGGYG	1 cut(s)	405
SinI	GGWCC	3 cut(s)	1253, 1307, 2675
SlaI	CTCGAG	1 cut(s)	2445
SmiMI	CAYNNNNRTG	2 cut(s)	898, 2543
SmlI	CTYRAG	3 cut(s)	573, 836, 2445
SmoI	CTYRAG	3 cut(s)	573, 836, 2445
SphI	GCATGC	1 cut(s)	2580
SseBI	AGGCCT	1 cut(s)	446
SspDI	GGCGCC	1 cut(s)	408
SspI	AATATT	2 cut(s)	1600, 2489
SspMI	CTAG	4 cut(s)	665, 1145, 1371, 2567
SstI	GAGCTC	1 cut(s)	2244
StuI	AGGCCT	1 cut(s)	446
StyD4I	CCNGG	5 cut(s)	1052, 1220, 1277, 2181, 2671
StyI	CCWWGG	3 cut(s)	702, 1370, 2494
TaaI	ACNGT	8 cut(s)	207, 487, 943, 954, 1571, 2003, 2542, 2656
TaiI	ACGT	1 cut(s)	330
TaqI	TCGA	6 cut(s)	90, 420, 620, 983, 2028, 2446
TaqII	GACCGA	1 cut(s)	337
TatI	WGTACW	4 cut(s)	1848, 2095, 2281, 2681
TauI	GCSGC	2 cut(s)	386, 688
TscAI	CASTG	5 cut(s)	946, 1053, 1809, 1968, 2068
TseFI	GTSAC	4 cut(s)	53, 355, 487, 1267
TseI	GCWGC	6 cut(s)	362, 386, 511, 1910, 2261, 2689
Tsp45I	GTSAC	4 cut(s)	53, 355, 487, 1267
TspGWI	ACGGA	3 cut(s)	343, 513, 733
TspRI	CASTG	5 cut(s)	946, 1053, 1809, 1968, 2068
Van91I	CCANNNNNTGG	1 cut(s)	1227
VpaK11BI	GGWCC	3 cut(s)	1253, 1307, 2675
VspI	ATTAAT	1 cut(s)	2345
XapI	RAATTY	4 cut(s)	141, 1006, 1510, 1790
XbaI	TCTAGA	1 cut(s)	2566
XceI	RCATGY	1 cut(s)	2580
XhoI	CTCGAG	1 cut(s)	2445
XmaJI	CCTAGG	1 cut(s)	1370
XmiI	GTMKAC	2 cut(s)	1073, 1875
XmnI	GAANNNNTTC	1 cut(s)	2402
XspI	CTAG	4 cut(s)	665, 1145, 1371, 2567
Zsp2I	ATGCAT	1 cut(s)	1825

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.