Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011760

Physical Location & Seq

Genomic Coordinates Reverse (-)

10923982 .. 10928399

Gene Structure 4418 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1147100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2733 bp

ATGCCACCTCTCAGTCTGCTGATGATCCTCTTGCTCACGTTGCTCTACTTCACCACCACCGCCACTTGTTACCGCCACCTCCTCCACCAGCCCCTTTTCCCCTTAGAGTCTTCCCCTCCAACCCAACCTCCATTCTTCTCTCCCCAGTCCCACCCAACCCAAATTCCCAAACTACCCTTCTCTTCCTCCTCCTCAACCCCTCCCCTAAAACCTTTCTTTCCCTCCTCCTCCTCTCACTCCCCACCGCCCCCTCCCTCCCCTACTTCCCTCACCTCCTTCCCCGCCAACATTTCCTCTATCCTCTTACCCAAAAACTCCTCCTCCTCCTCCTCTTCCCACCGCCACGTCATTGCCATCGCCATCTCTGTTTCTGTCACTGCCTTCATCCTCATCGCCTCCGCCGCTGCATTTCTGTACTACCGTCGACGCCGGCAGCCTAACCCTACCATACCCGACGATAAGGCCTCCAGAAACGACAGTCTCCGTCTGTTCCCGCCAAACACCGCCTCCTCCGACGCGGTTCACAATCATCGTAGTAGTACGCCCAATACCAGCAGAGAGTTCCTCTACCTCGGAACCTTAGTTAGTCCCCGACAAGGAACCGACGAGGAAAACGTTGCGAACACGAGCAATGCGGGCTTGGCGTTCGGGGTGTCGAGCCACTCTGCCTCGCCGCCGTATCAGAGACTGGGCTCGCCGGAGCTGAAGCCACTTCCGCCACTTCCCAAGCACAATTTCAGACGGAGCTTCAAGAATTCCCAACTGGGTTCCGATGATGATGAAGAGGAGTTCTTTTCCCCCAGAGGGTCTTCCGCGAGTCCCAAACATGGTAGCGGAAGAATAACAAGCCCTGACAGAGTGTTTAAAGCCGTCGATGGCGTAAACATCGGAAGTAGAAGCTTCAATTCGAGAACTGCGTCATACCCATGTTCGAAATCTGCTTCCCCAGCAAGTTCTTGGTCTCACACGGTCTCGCCGGTGTTGAATTTGAGCCCCAGAAGCTTGAAATCGAAGTCGCCGGACTTAGTAATCAATTTCTCAGCTCCGTCGCGGCCTCCTCCGATACCAGTGTCATCGTCCCCATGCTTCTCTTCGTCGTCCTCAAGAAATTCTGACCTTATCGGGCTGAAGAACCACTCTCCAGAGATAAATAAGAGTTTTGTTCCGGTAAGACTTCCACCTCCGCCTCCGCCACTGCCACCACCGAGGTTTTGGGAAGCCCCGGCGGGTCCAAGTCGCAACACAGGGCCACCGGTTCTTATCACTCCTTCCCGGCCGGCTGTGCCTCAGAATTCGGGACCCATTTTGGCGGGTGAAAGTTCAGAGAGCAACGAGATGGTGGAAAAGAATGATGAGACTCCAACAAAGCCAAAGCTGAAACCTTTACATTGGGATAAAGTTAGGGCCAGCTCAGACCGCGCCATGGTGTGGGATCACTTGAAATCCAGCTCTTTTCAGTTGAATGAGGAGATGATTGAGACATTGTTTATGGTAAACAATTCGGGTTTGACACCGAATGACAATGTCCGGCGACAGATTCTTCCTTCTGTGAATCAGGAGAACCGTGTGCTTGATCCGAAGAAGTCTCAGAACATTGCAATTTTGTTGAGGGCGCTTAATGTTACCATTGATGAAGTCTGTGAAGCGCTTTTGGAAGGTAATTCAGATGGACTTGGGACAGAACTTCTTGAAAGTCTATTAAAGATGGCTCCAACCAAAGAGGAAGAACGGAAACTAAGAGAGTTCAAAGATGAATCACCTTTTAAGCTTGGCCCAGCTGAGAAATTTCTGAAGGCGGTGCTCGATATACCTTTCGCGTTTAAGAGGGTGGATGCAATGCTCTACATTGCCAATTTTGATTCAGAAGTAGATTATCTGAAAAGGTCTTTCGAGACTGTGGGGGCAGCTTGCGAAGAATTAAGGAACAGCAAAATGTTCCTGAAGCTTCTTGAAGCGGTGCTCAAAACTGGGAACCGGATGAATGTTGGCACTAACCGTGGCGATGCCCATGCCTTCAAGCTAGACACACTTCTCAAGCTTGTAGATGTGAAGGGAACAGATGGGAAAACTACTCTCTTACATTTTGTTGTGCAGGAAATCATCAGAGCTGAAGGTTCTCGTCTCTCAGGCATGAATCAGAATCAAATAGCTGAGGAAAGCCAACAAACTTCCTTCCGGGATGATGTCGAGTTTAGGAAGCTTGGCCTGCAAGTTGTTTCAGGATTGAGTGGGGAGCTCACCAGTGTAAAGAAGGCTGCTACGATGGATTCAGATGTACTTTGCAATGAAGTTGCAAAAATTGCTGCTGGAATTACGAAAATAGCAGAAGTTGTAAAATTGATCGAAGGCAGTGCATCGAAGGAGAGTAGCAAAAAGTTCTTCGAGTCCATGAGCGGGTTCTTGAACAAGGCAGGGGAAGAAATTGTAAGGATTCAAGCTCAAGAGAAAGTTGCATTCTCTATGGTAAAGGACTTAACAGAATATTTCCATGGGAACTCGGTAAGGGAAGAAGCTCATCCCTTCCGAATTTTCACGGTGGTGAGAGATTTTCTTTCTATTCTAGATCAGGCGTGCAAGGTGGTCGGAAAAGTTAATGAAAGAACTATAGTTGGGTCAGCCCGTCAATTCCCCAGGGTTCCGAATGCAACCCTTCCACCGTTTTTTCCAGAGCTCGGTGCAAAGCAGCATTATGGTTCCTCGGATGATGAAACCGCGCTTCGACATAGTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009987 GO:0010638 GO:0010639 GO:0016020 GO:0016043 GO:0030029 GO:0030036 GO:0030312 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0030838 GO:0031333 GO:0031334 GO:0032271 GO:0032272 GO:0032273 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0043242 GO:0043244 GO:0043254 GO:0044087 GO:0044089 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051015 GO:0051016 GO:0051128 GO:0051129 GO:0051130 GO:0051493 GO:0051494 GO:0051495 GO:0051693 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0097435 GO:0110053 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04320 map04320

Pfam Domains

Protein Families

FH2

Protein Analysis

911

Amino Acids

99.71

Weight (kDa)

9.04

Isoelectric Point (pI)

58.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	451 - 854	4.9e-115	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011760)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43800
fragaria_vesca	FvH4_1g00460
malus_domestica	MD02G1003800.v1.1 MD15G1147100.v1.1
prunus_persica	Prupe.7G267500_v2.0.a1
pyrus_communis	pycom02g00230 pycom15g13200
rosa_chinensis	RchiOBHm_Chr2g0084971
rosa_laevigata	RLG00000015652
rosa_multiflora	Rmu_co8206718.1_g000001 Rmu_sc0017508.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155670
rosa_rugosa	Rorug01G0457400
rosa_samantha	Rh2AG005000 Rh2BG006200 Rh2BG300200 Rh2CG006800 Rh2DG006600
rosa_wichuraiana	Rw2G000500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1428
AccBSI	CCGCTC	1 cut(s)	2394
AccI	GTMKAC	1 cut(s)	424
AccII	CGCG	6 cut(s)	518, 815, 1051, 1419, 1817, 2714
AclI	AACGTT	1 cut(s)	615
AclWI	GGATC	3 cut(s)	19, 1440, 1568
AcoI	YGGCCR	1 cut(s)	1274
AcsI	RAATTY	7 cut(s)	162, 754, 985, 1108, 1291, 1784, 2526
AcuI	CTGAAG	5 cut(s)	725, 1148, 1811, 1961, 2130
AcyI	GRCGYC	1 cut(s)	427
AfaI	GTAC	3 cut(s)	416, 541, 2277
AfeI	AGCGCT	1 cut(s)	1647
AfiI	CCNNNNNNNGG	7 cut(s)	596, 804, 827, 1423, 1428, 2394, 2672
AgeI	ACCGGT	1 cut(s)	1252
AjiI	CACGTC	1 cut(s)	346
AjnI	CCWGG	1 cut(s)	2630
AjuI	GAANNNNNNNTTGG	4 cut(s)	161, 193, 719, 751
AleI	CACNNNNGTG	1 cut(s)	2537
Alw21I	GWGCWC	4 cut(s)	1803, 1962, 2238, 2673
Alw26I	GTCTC	9 cut(s)	485, 679, 966, 976, 1349, 1472, 1590, 1886, 2126
AlwI	GGATC	3 cut(s)	19, 1440, 1568
AlwNI	CAGNNNCTG	1 cut(s)	688
Aor51HI	AGCGCT	1 cut(s)	1647
AoxI	GGCC	7 cut(s)	462, 1052, 1247, 1274, 1404, 1771, 2203
ApeKI	GCWGC	6 cut(s)	404, 433, 1904, 2255, 2303, 2683
ApoI	RAATTY	7 cut(s)	162, 754, 985, 1108, 1291, 1784, 2526
AsiGI	ACCGGT	1 cut(s)	1252
AspLEI	GCGC	4 cut(s)	1421, 1615, 1648, 2716
AspS9I	GGNCC	5 cut(s)	1229, 1247, 1298, 1404, 1772
AsuC2I	CCSGG	3 cut(s)	1223, 1273, 2177
AsuHPI	GGTGA	6 cut(s)	43, 262, 1325, 1749, 2230, 2551
AsuII	TTCGAA	1 cut(s)	932
AvaII	GGWCC	2 cut(s)	1229, 1298
BaeI	ACNNNNGTAYC	2 cut(s)	1055, 1088
BanII	GRGCYC	4 cut(s)	695, 995, 2238, 2673
BauI	CACGAG	1 cut(s)	625
BbsI	GAAGAC	2 cut(s)	102, 801
Bbv12I	GWGCWC	4 cut(s)	1803, 1962, 2238, 2673
BbvCI	CCTCAGC	1 cut(s)	2151
BbvI	GCAGC	6 cut(s)	391, 445, 1916, 2242, 2290, 2695
BccI	CCATC	8 cut(s)	362, 368, 869, 1330, 1661, 1699, 2054, 2257
BceAI	ACGGC	2 cut(s)	661, 854
BcgI	CGANNNNNNTGC	6 cut(s)	1991, 2025, 2333, 2367, 2563, 2597
BciT130I	CCWGG	1 cut(s)	2632
BcnI	CCSGG	3 cut(s)	1223, 1273, 2177
BcoDI	GTCTC	9 cut(s)	485, 679, 966, 976, 1349, 1472, 1590, 1886, 2126
BfaI	CTAG	2 cut(s)	2021, 2561
BfmI	CTRYAG	1 cut(s)	2604
BfoI	RGCGCY	2 cut(s)	1616, 1649
BisI	GCNGC	9 cut(s)	402, 405, 434, 674, 1052, 1905, 2256, 2304, 2684
BlsI	GCNGC	9 cut(s)	403, 406, 435, 675, 1053, 1906, 2257, 2305, 2685
Bme1390I	CCNGG	4 cut(s)	1223, 1273, 2177, 2632
Bme18I	GGWCC	2 cut(s)	1229, 1298
BmgBI	CACGTC	1 cut(s)	346
BmgT120I	GGNCC	5 cut(s)	1229, 1247, 1298, 1404, 1772
BmrFI	CCNGG	4 cut(s)	1223, 1273, 2177, 2632
BmrI	ACTGGG	4 cut(s)	139, 698, 773, 1977
BmsI	GCATC	3 cut(s)	1822, 1993, 2363
BmuI	ACTGGG	4 cut(s)	139, 698, 773, 1977
BpiI	GAAGAC	2 cut(s)	102, 801
BplI	GAGNNNNNCTC	2 cut(s)	549, 581
BpmI	CTGGAG	2 cut(s)	451, 1125
Bpu10I	CCTNAGC	1 cut(s)	2151
Bpu14I	TTCGAA	1 cut(s)	932
BpuEI	CTTGAG	3 cut(s)	1087, 2018, 2424
BpuMI	CCSGG	3 cut(s)	1223, 1273, 2177
BsaHI	GRCGYC	1 cut(s)	427
BsaI	GGTCTC	2 cut(s)	966, 976
BsaJI	CCNNGG	9 cut(s)	571, 1205, 1221, 1422, 1996, 2488, 2630, 2631, 2697
BsaWI	WCCGGW	3 cut(s)	1165, 1252, 1974
BsaXI	ACNNNNNCTCC	6 cut(s)	112, 142, 305, 335, 491, 521
Bsc4I	CCNNNNNNNGG	7 cut(s)	596, 804, 827, 1423, 1428, 2394, 2672
Bse118I	RCCGGY	4 cut(s)	429, 976, 1252, 1276
Bse1I	ACTGG	6 cut(s)	145, 693, 768, 1067, 1972, 2241
Bse3DI	GCAATG	6 cut(s)	348, 637, 1593, 1842, 1845, 2290
BseBI	CCWGG	1 cut(s)	2632
BseDI	CCNNGG	9 cut(s)	571, 1205, 1221, 1422, 1996, 2488, 2630, 2631, 2697
BseGI	GGATG	6 cut(s)	384, 1837, 1983, 2185, 2515, 2707
BseLI	CCNNNNNNNGG	7 cut(s)	596, 804, 827, 1423, 1428, 2394, 2672
BseMI	GCAATG	6 cut(s)	348, 637, 1593, 1842, 1845, 2290
BseMII	CTCAG	8 cut(s)	25, 1053, 1301, 1425, 1601, 1770, 2139, 2142
BseNI	ACTGG	6 cut(s)	145, 693, 768, 1067, 1972, 2241
BseX3I	CGGCCG	1 cut(s)	1274
BseXI	GCAGC	6 cut(s)	391, 445, 1916, 2242, 2290, 2695
BseYI	CCCAGC	2 cut(s)	946, 1774
BsgI	GTGCAG	1 cut(s)	2111
Bsh1236I	CGCG	6 cut(s)	518, 815, 1051, 1419, 1817, 2714
Bsh1285I	CGRYCG	1 cut(s)	1277
BshFI	GGCC	7 cut(s)	464, 1054, 1249, 1276, 1406, 1773, 2205
BshTI	ACCGGT	1 cut(s)	1252
BsiEI	CGRYCG	1 cut(s)	1277
BsiHKAI	GWGCWC	4 cut(s)	1803, 1962, 2238, 2673
BslFI	GGGAC	6 cut(s)	133, 573, 804, 1063, 1311, 1690
BslI	CCNNNNNNNGG	7 cut(s)	596, 804, 827, 1423, 1428, 2394, 2672
BsmAI	GTCTC	9 cut(s)	485, 679, 966, 976, 1349, 1472, 1590, 1886, 2126
BsmBI	CGTCTC	1 cut(s)	2126
BsmFI	GGGAC	6 cut(s)	133, 573, 804, 1063, 1311, 1690
BsmI	GAATGC	2 cut(s)	2453, 2647
BsnI	GGCC	7 cut(s)	464, 1054, 1249, 1276, 1406, 1773, 2205
Bso31I	GGTCTC	2 cut(s)	966, 976
Bsp119I	TTCGAA	1 cut(s)	932
Bsp1286I	GDGCHC	6 cut(s)	695, 995, 1803, 1962, 2238, 2673
Bsp143I	GATC	5 cut(s)	24, 1432, 1573, 2340, 2563
Bsp19I	CCATGG	2 cut(s)	1422, 2488
BspANI	GGCC	7 cut(s)	464, 1054, 1249, 1276, 1406, 1773, 2205
BspCNI	CTCAG	8 cut(s)	24, 1052, 1300, 1424, 1600, 1771, 2138, 2143
BspFNI	CGCG	6 cut(s)	518, 815, 1051, 1419, 1817, 2714
BspPI	GGATC	3 cut(s)	19, 1440, 1568
BspT104I	TTCGAA	1 cut(s)	932
BspTNI	GGTCTC	2 cut(s)	966, 976
BsrBI	CCGCTC	1 cut(s)	2394
BsrDI	GCAATG	6 cut(s)	348, 637, 1593, 1842, 1845, 2290
BsrFI	RCCGGY	4 cut(s)	429, 976, 1252, 1276
BsrI	ACTGG	6 cut(s)	145, 693, 768, 1067, 1972, 2241
BssAI	RCCGGY	4 cut(s)	429, 976, 1252, 1276
BssECI	CCNNGG	9 cut(s)	571, 1205, 1221, 1422, 1996, 2488, 2630, 2631, 2697
BssMI	GATC	5 cut(s)	24, 1432, 1573, 2340, 2563
BssNI	GRCGYC	1 cut(s)	427
BssSI	CACGAG	1 cut(s)	625
BssT1I	CCWWGG	2 cut(s)	1422, 2488
Bst2BI	CACGAG	1 cut(s)	625
Bst2UI	CCWGG	1 cut(s)	2632
Bst4CI	ACNGT	8 cut(s)	422, 479, 970, 1565, 1897, 1997, 2536, 2658
Bst6I	CTCTTC	4 cut(s)	187, 337, 777, 1096
BstACI	GRCGYC	1 cut(s)	427
BstAPI	GCANNNNNTGC	1 cut(s)	2300
BstBI	TTCGAA	1 cut(s)	932
BstC8I	GCNNGC	8 cut(s)	431, 637, 695, 1278, 1408, 1909, 2207, 2572
BstDSI	CCRYGG	3 cut(s)	1422, 1996, 2488
BstF5I	GGATG	6 cut(s)	384, 1837, 1983, 2185, 2515, 2707
BstFNI	CGCG	6 cut(s)	518, 815, 1051, 1419, 1817, 2714
BstH2I	RGCGCY	2 cut(s)	1616, 1649
BstHHI	GCGC	4 cut(s)	1421, 1615, 1648, 2716
BstKTI	GATC	5 cut(s)	27, 1435, 1576, 2343, 2566
BstMAI	GTCTC	9 cut(s)	485, 679, 966, 976, 1349, 1472, 1590, 1886, 2126
BstMBI	GATC	5 cut(s)	24, 1432, 1573, 2340, 2563
BstMCI	CGRYCG	1 cut(s)	1277
BstNI	CCWGG	1 cut(s)	2632
BstSCI	CCNGG	4 cut(s)	1221, 1271, 2175, 2630
BstSFI	CTRYAG	1 cut(s)	2604
BstUI	CGCG	6 cut(s)	518, 815, 1051, 1419, 1817, 2714
BstV1I	GCAGC	6 cut(s)	391, 445, 1916, 2242, 2290, 2695
BstV2I	GAAGAC	2 cut(s)	102, 801
BstZI	CGGCCG	1 cut(s)	1274
BsuRI	GGCC	7 cut(s)	464, 1054, 1249, 1276, 1406, 1773, 2205
BtgI	CCRYGG	3 cut(s)	1422, 1996, 2488
BtgZI	GCGATG	3 cut(s)	340, 376, 2016
BtrI	CACGTC	1 cut(s)	346
BtsCI	GGATG	6 cut(s)	384, 1837, 1983, 2185, 2515, 2707
BtsI	GCAGTG	3 cut(s)	375, 1193, 2356
BtsIMutI	CAGTG	5 cut(s)	375, 1074, 1193, 2248, 2356
Cac8I	GCNNGC	8 cut(s)	431, 637, 695, 1278, 1408, 1909, 2207, 2572
CaiI	CAGNNNCTG	1 cut(s)	688
CfoI	GCGC	4 cut(s)	1421, 1615, 1648, 2716
Cfr10I	RCCGGY	4 cut(s)	429, 976, 1252, 1276
Cfr13I	GGNCC	5 cut(s)	1229, 1247, 1298, 1404, 1772
CseI	GACGC	3 cut(s)	435, 524, 906
Csp6I	GTAC	3 cut(s)	415, 540, 2276
CspAI	ACCGGT	1 cut(s)	1252
CviAII	CATG	8 cut(s)	827, 927, 1083, 1423, 2009, 2131, 2389, 2489
CviQI	GTAC	3 cut(s)	415, 540, 2276
DpnI	GATC	5 cut(s)	26, 1434, 1575, 2342, 2565
DpnII	GATC	5 cut(s)	24, 1432, 1573, 2340, 2563
DraI	TTTAAA	1 cut(s)	865
EaeI	YGGCCR	1 cut(s)	1274
EagI	CGGCCG	1 cut(s)	1274
Eam1104I	CTCTTC	4 cut(s)	187, 337, 777, 1096
EarI	CTCTTC	4 cut(s)	187, 337, 777, 1096
EciI	GGCGGA	4 cut(s)	388, 705, 1173, 1179
Ecl136II	GAGCTC	2 cut(s)	2236, 2671
EclXI	CGGCCG	1 cut(s)	1274
Eco130I	CCWWGG	2 cut(s)	1422, 2488
Eco147I	AGGCCT	1 cut(s)	464
Eco24I	GRGCYC	4 cut(s)	695, 995, 2238, 2673
Eco31I	GGTCTC	2 cut(s)	966, 976
Eco47I	GGWCC	2 cut(s)	1229, 1298
Eco47III	AGCGCT	1 cut(s)	1647
Eco52I	CGGCCG	1 cut(s)	1274
Eco53kI	GAGCTC	2 cut(s)	2236, 2671
Eco57I	CTGAAG	5 cut(s)	725, 1148, 1811, 1961, 2130
EcoICRI	GAGCTC	2 cut(s)	2236, 2671
EcoO109I	RGGNCCY	1 cut(s)	1298
EcoRI	GAATTC	2 cut(s)	754, 1291
EcoRII	CCWGG	1 cut(s)	2630
EcoT14I	CCWWGG	2 cut(s)	1422, 2488
EcoT38I	GRGCYC	4 cut(s)	695, 995, 2238, 2673
ErhI	CCWWGG	2 cut(s)	1422, 2488
Esp3I	CGTCTC	1 cut(s)	2126
FaeI	CATG	8 cut(s)	830, 930, 1086, 1426, 2012, 2134, 2392, 2492
FaqI	GGGAC	6 cut(s)	133, 573, 804, 1063, 1311, 1690
FatI	CATG	8 cut(s)	826, 926, 1082, 1422, 2008, 2130, 2388, 2488
FauI	CCCGC	6 cut(s)	289, 501, 628, 1219, 1303, 2387
FblI	GTMKAC	1 cut(s)	424
Fnu4HI	GCNGC	9 cut(s)	402, 405, 434, 674, 1052, 1905, 2256, 2304, 2684
FokI	GGATG	6 cut(s)	371, 1844, 1990, 2192, 2502, 2714
FriOI	GRGCYC	4 cut(s)	695, 995, 2238, 2673
Fsp4HI	GCNGC	9 cut(s)	402, 405, 434, 674, 1052, 1905, 2256, 2304, 2684
FspBI	CTAG	2 cut(s)	2021, 2561
GlaI	GCGC	4 cut(s)	1420, 1614, 1647, 2715
GluI	GCNGC	9 cut(s)	402, 405, 434, 674, 1052, 1905, 2256, 2304, 2684
GsaI	CCCAGC	2 cut(s)	950, 1778
GsuI	CTGGAG	2 cut(s)	451, 1125
HaeII	RGCGCY	2 cut(s)	1616, 1649
HaeIII	GGCC	7 cut(s)	464, 1054, 1249, 1276, 1406, 1773, 2205
HgaI	GACGC	3 cut(s)	435, 524, 906
HhaI	GCGC	4 cut(s)	1421, 1615, 1648, 2716
Hin1I	GRCGYC	1 cut(s)	427
Hin1II	CATG	8 cut(s)	830, 930, 1086, 1426, 2012, 2134, 2392, 2492
Hin6I	GCGC	4 cut(s)	1419, 1613, 1646, 2714
HinP1I	GCGC	4 cut(s)	1419, 1613, 1646, 2714
HincII	GTYRAC	1 cut(s)	425
HindII	GTYRAC	1 cut(s)	425
HindIII	AAGCTT	6 cut(s)	898, 1000, 1766, 1943, 2036, 2198
HphI	GGTGA	6 cut(s)	43, 262, 1325, 1749, 2230, 2551
Hpy166II	GTNNAC	4 cut(s)	425, 523, 883, 1495
Hpy8I	GTNNAC	4 cut(s)	425, 523, 883, 1495
Hpy99I	CGWCG	8 cut(s)	426, 429, 458, 518, 608, 875, 1051, 1099
HpyCH4III	ACNGT	8 cut(s)	422, 479, 970, 1565, 1897, 1997, 2536, 2658
HpyCH4IV	ACGT	3 cut(s)	38, 345, 615
HpySE526I	ACGT	3 cut(s)	38, 345, 615
Hsp92I	GRCGYC	1 cut(s)	427
Hsp92II	CATG	8 cut(s)	830, 930, 1086, 1426, 2012, 2134, 2392, 2492
HspAI	GCGC	4 cut(s)	1419, 1613, 1646, 2714
KflI	GGGWCCC	1 cut(s)	1298
KroI	GCCGGC	2 cut(s)	429, 1276
KroNI	GCCGGC	2 cut(s)	431, 1278
Kzo9I	GATC	5 cut(s)	24, 1432, 1573, 2340, 2563
LmnI	GCTCC	5 cut(s)	700, 744, 1048, 1714, 2233
Lsp1109I	GCAGC	6 cut(s)	391, 445, 1916, 2242, 2290, 2695
LweI	GCATC	3 cut(s)	1822, 1993, 2363
MaeI	CTAG	2 cut(s)	2021, 2561
MaeII	ACGT	3 cut(s)	38, 345, 615
MaeIII	GTNAC	3 cut(s)	68, 373, 1621
MalI	GATC	5 cut(s)	26, 1434, 1575, 2342, 2565
MbiI	CCGCTC	1 cut(s)	2394
MboI	GATC	5 cut(s)	24, 1432, 1573, 2340, 2563
MhlI	GDGCHC	6 cut(s)	695, 995, 1803, 1962, 2238, 2673
MlyI	GAGTC	4 cut(s)	116, 826, 1351, 2393
MmeI	TCCRAC	5 cut(s)	143, 537, 1385, 1736, 2563
MroNI	GCCGGC	2 cut(s)	429, 1276
MseI	TTAA	8 cut(s)	864, 1617, 1700, 1764, 1821, 1919, 2474, 2592
MslI	CAYNNNNRTG	2 cut(s)	925, 2537
MspA1I	CMGCKG	2 cut(s)	404, 1778
MspR9I	CCNGG	4 cut(s)	1223, 1273, 2177, 2632
Mva1269I	GAATGC	2 cut(s)	2453, 2647
MvaI	CCWGG	1 cut(s)	2632
MvnI	CGCG	6 cut(s)	518, 815, 1051, 1419, 1817, 2714
NaeI	GCCGGC	2 cut(s)	431, 1278
NciI	CCSGG	3 cut(s)	1223, 1273, 2177
NcoI	CCATGG	2 cut(s)	1422, 2488
NdeII	GATC	5 cut(s)	24, 1432, 1573, 2340, 2563
NgoMIV	GCCGGC	2 cut(s)	429, 1276
NlaIII	CATG	8 cut(s)	830, 930, 1086, 1426, 2012, 2134, 2392, 2492
NmuCI	GTSAC	1 cut(s)	373
NspV	TTCGAA	1 cut(s)	932
OliI	CACNNNNGTG	1 cut(s)	2537
PasI	CCCWGGG	1 cut(s)	2631
PceI	AGGCCT	1 cut(s)	464
PcsI	WCGNNNNNNNCGW	2 cut(s)	612, 914
PctI	GAATGC	2 cut(s)	2453, 2647
PdiI	GCCGGC	2 cut(s)	431, 1278
PfeI	GAWTC	8 cut(s)	1537, 1552, 1754, 1859, 2134, 2140, 2267, 2431
PflFI	GACNNNGTC	1 cut(s)	1523
PflMI	CCANNNNNTGG	1 cut(s)	1428
PfoI	TCCNGGA	1 cut(s)	2175
PinAI	ACCGGT	1 cut(s)	1252
PkrI	GCNGC	9 cut(s)	403, 406, 435, 675, 1053, 1906, 2257, 2305, 2685
PleI	GAGTC	4 cut(s)	115, 825, 1351, 2392
PpsI	GAGTC	4 cut(s)	115, 825, 1351, 2392
PpuMI	RGGWCCY	1 cut(s)	1298
Psp124BI	GAGCTC	2 cut(s)	2238, 2673
Psp1406I	AACGTT	1 cut(s)	615
Psp5II	RGGWCCY	1 cut(s)	1298
Psp6I	CCWGG	1 cut(s)	2630
PspFI	CCCAGC	2 cut(s)	946, 1774
PspGI	CCWGG	1 cut(s)	2630
PspPI	GGNCC	5 cut(s)	1229, 1247, 1298, 1404, 1772
PspPPI	RGGWCCY	1 cut(s)	1298
PstNI	CAGNNNCTG	1 cut(s)	688
PsyI	GACNNNGTC	1 cut(s)	1523
PvuII	CAGCTG	1 cut(s)	1778
RsaI	GTAC	3 cut(s)	416, 541, 2277
RsaNI	GTAC	3 cut(s)	415, 540, 2276
RseI	CAYNNNNRTG	2 cut(s)	925, 2537
SacI	GAGCTC	2 cut(s)	2238, 2673
SalI	GTCGAC	1 cut(s)	423
SaqAI	TTAA	8 cut(s)	864, 1617, 1700, 1764, 1821, 1919, 2474, 2592
SatI	GCNGC	9 cut(s)	402, 405, 434, 674, 1052, 1905, 2256, 2304, 2684
Sau3AI	GATC	5 cut(s)	24, 1432, 1573, 2340, 2563
Sau96I	GGNCC	5 cut(s)	1229, 1247, 1298, 1404, 1772
SchI	GAGTC	4 cut(s)	116, 826, 1351, 2393
ScrFI	CCNGG	4 cut(s)	1223, 1273, 2177, 2632
SduI	GDGCHC	6 cut(s)	695, 995, 1803, 1962, 2238, 2673
SfaNI	GCATC	3 cut(s)	1822, 1993, 2363
SfcI	CTRYAG	1 cut(s)	2604
SfuI	TTCGAA	1 cut(s)	932
SgrAI	CRCCGGYG	1 cut(s)	976
SgrDI	CGTCGACG	1 cut(s)	423
SinI	GGWCC	2 cut(s)	1229, 1298
SmiMI	CAYNNNNRTG	2 cut(s)	925, 2537
SmlI	CTYRAG	3 cut(s)	1102, 2033, 2439
SmoI	CTYRAG	3 cut(s)	1102, 2033, 2439
SseBI	AGGCCT	1 cut(s)	464
SspI	AATATT	1 cut(s)	2483
SspMI	CTAG	2 cut(s)	2021, 2561
SstI	GAGCTC	2 cut(s)	2238, 2673
StuI	AGGCCT	1 cut(s)	464
StyD4I	CCNGG	4 cut(s)	1221, 1271, 2175, 2630
StyI	CCWWGG	2 cut(s)	1422, 2488
TaaI	ACNGT	8 cut(s)	422, 479, 970, 1565, 1897, 1997, 2536, 2658
TaiI	ACGT	3 cut(s)	41, 348, 618
TatI	WGTACW	2 cut(s)	414, 2275
TauI	GCSGC	3 cut(s)	404, 676, 1054
TfiI	GAWTC	8 cut(s)	1537, 1552, 1754, 1859, 2134, 2140, 2267, 2431
Tru1I	TTAA	8 cut(s)	864, 1617, 1700, 1764, 1821, 1919, 2474, 2592
Tru9I	TTAA	8 cut(s)	864, 1617, 1700, 1764, 1821, 1919, 2474, 2592
TscAI	CASTG	5 cut(s)	382, 1074, 1200, 2248, 2356
TseFI	GTSAC	1 cut(s)	373
TseI	GCWGC	6 cut(s)	404, 433, 1904, 2255, 2303, 2683
Tsp45I	GTSAC	1 cut(s)	373
TspDTI	ATGAA	9 cut(s)	373, 795, 1647, 1767, 1994, 2147, 2301, 2610, 2721
TspGWI	ACGGA	4 cut(s)	473, 757, 1035, 1744
TspRI	CASTG	5 cut(s)	382, 1074, 1200, 2248, 2356
Tth111I	GACNNNGTC	1 cut(s)	1523
Van91I	CCANNNNNTGG	1 cut(s)	1428
VpaK11BI	GGWCC	2 cut(s)	1229, 1298
XapI	RAATTY	7 cut(s)	162, 754, 985, 1108, 1291, 1784, 2526
XbaI	TCTAGA	1 cut(s)	2560
XcmI	CCANNNNNNNNNTGG	2 cut(s)	954, 1209
XmiI	GTMKAC	1 cut(s)	424
XspI	CTAG	2 cut(s)	2021, 2561

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.