Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0011760

Physical Location & Seq

Genomic Coordinates Reverse (-)

461084 .. 465045

Gene Structure 3962 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G000500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2745 bp

ATGGCAGCTCTCAGTACTCTCACTCTGATCATCTTCCTCCTCCGCTTCACCACCGCCACCTGTCACCGCCACCTCCTCCACCAGCCCTTCTTCCCAGTTGAATCAAACCCTCCAACCCAACCACCATCTTCCTCTCCCCACCCCTCTCAAATTCCCAAAATGCCCTTCTCCTCCACCTCATCCTCCTCCTCGACGCCACCTGATAATAACAAACCGTTCTTTCCCTCCATCAAACCCACGCCGCCTCCTCCCTCCCCAACAGCCCTCACCTCCTTCCCCGCCAACATTTCCTCCATCCTTTTCCCCCAACCCTCTTCCTCCTCCCCCCACCGCCACGTCATCGCCATCGCCGTCTCGGTCTCCGTCACGGCTGCACTCATCATCGCCTTCGCCGCTGCTTTTTTACTCTACCGCCGCCGCCGGAAGCTCGACCCGACCTCGGCCGACGACAAGGCCTCCAGAACCGACAGCCTCCGCCTGTTCCCGCCCAACACCGTCACCTCCGACGGAGTGCACAAGCAGCGTACCACACCCAACAGAACCACCGAGAACTTCCTCTACCTCGGCACATTATCCAACGCTCATGGAAACGACGAACAAAGCGTCGCCACCACGAGCAATGCCAGCTCCAAGTTCGGGGTTTCAAGCCGGTCTGGCTCGCCGTATCAGAAGCTGGGCTCGCCGGAGCTGACGCCGCTTCCTCCACTTCCCAAGGACAATTTCATACGACGGAGCTACAAGCACGAACTGGGTTCCGACGATGATGAAGGGGAAGACGAGTTCTTTTCTCCGAGAGGCTCTTCTGCGAGTCCCAAAAACGTCGCCGGAGCAAGCTCAGATAGAGTGCTCAAGGCTGTAGAAGGCGACAACTTTGGAAGCAGGAGCTTCAATTCAAGAACTGCATCATACCCATGTTCAAAATCTGCTTCTCCAGTAAGTTCTGTGTCCAACACTGTCTCACCACAGTTGAACTTCAGTCCCACAAGCTTGAAATCGAACTCGCCGGACTCTGTGATCAATTTCATGGCACCGTCTCGGCCTGCTCCGATTCCGGTGACAGTGTCCCGGTCATTGTCTTCGTCGTCTACTTTATCAGAGAGAGTTTCAGGGAATGTGCAGAGTTCCCCCGACTTTTTGACGCAGATGAAGCAGTCTCTAGTGAGAAACAATAGCGCTCCTAAGCGGTTTGTTCCAATAAGACTGCCTCCGCCGCCTCCTCCACCGCCACCACCCAGGTATTGGGAAGCTTCAGGGACTCCGGTGGCGAATGCAGGTCCACCTGTTCTTGTCACGCCTTCCGGGGCTCCTGTGTTTGAGAATACGGGACCAGTTTTGGCTGGTGAACCATCACAGAAGAATGACATTGAGGAAAAAAGCGAGGAAACTCCTAGGCCAAAGTTGAAGCCTTTACATTGGGACAAAGTTAGGGCCAGCTCAGACCGCGCTATGGTGTGGGATCAGTTAAAATCGAGCTCCTTTCAGTTGAATGAGGAAATGATCGAGACACTTTTTGTGGTAAACAATTCAAGTTTGACACCGAAAGAAAATGCTCGGCGACAGATTCTACCCTCTTTGAATCAGGAGAACCGTGTGCTGGATCCAAAGAAGTCTCAGAATATTGCAATTTTGTTGAGGGCGCTCAATGTTACCATTGATGAAGTCTGTGAGGCCCTCTTGGAAGGTAATTCGGATTCCCTCGGAACGGAACTTCTTGAAAGTTTATTAAAGATGGCTCCAACCAAAGAAGAAGAATGGAAGCTAAAAGAGTTCAAAGATGAATCACCATTTAAGCTTGGCCCAGCTGAGAAATTTCTGAAGGCAGTGCTTGATGTACCTTATGCATTTAAGAGGGTGGATGCAATGCTGTACATTGCCAATTTTGATTCAGAAGTAGACTATCTTAAAAGGTCTTTTGAGACATTGGAGGCAGCTTGTGAAGAATTGAAGAACAGCAGAATGTTCTTTAAGCTTCTTGAAGCAGTGCTCAAAACTGGGAACCGCATGAATGTCGGCACCAACCGTGGCGATGCTCATGCCTTCAAGCTCGACACACTCCTGAAGCTGGTAGATGTGAAGGGCACTGATGGAAAAACTACTCTGTTGCATTTTGTAGTACAGGAGATCATCAGAGCTGAAGGTTCTCGTCTTTCGGGCGTGAATCAGGATGAAATGGCTGAGAGCAGTCAACAATCTTCTTTCCGGGATGATGTGGAGTTTAGGAAGCGTGGCCTGCAAGTTGTTTCAGGATTGAGTGGAGAGCTCATCAATGTAAAGAAAGCTGCTGGTATGGATTCGGATGTACTGAGCAACGAAGTAGCAAAGATTGCTGGGGGAATTACAAAGATTGAAGAAGTTGTAAAATTAATTGAAGGAAGTGCATTGAAGGAGAGTAGCCAGAAGTTCTTCGACTCCATGAATGGGTTCTTGAAGAAGGCAAGTGAAGAGATTGTGATGATTCAAGCTCGAGAGAAACTTGCATTCTGTATGGTGAAGGAAATAACAGAATATTTCCATGGGAACTCGGCTAAAGAAGAAGCTCATCCTTTCCGGATTTTCACGGTGGTGAGAGACTTCCTTTCAATTCTAGATCAGGCATGCAAGGTAGTTGGCAAAGTTAATGAAAGAACTATAGTTGGGTCTGCCCGTCAATTTCCAATTGTCATGAATCCAACCGTTCCACCAGTTTTTCCTGGACCAAGTACAAAGCAGCATTATGGTTCCTCGGATGATGAAACCTTGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009987 GO:0010638 GO:0010639 GO:0016020 GO:0016043 GO:0030029 GO:0030036 GO:0030312 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0030838 GO:0031333 GO:0031334 GO:0032271 GO:0032272 GO:0032273 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0043242 GO:0043244 GO:0043254 GO:0044087 GO:0044089 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051015 GO:0051016 GO:0051128 GO:0051129 GO:0051130 GO:0051493 GO:0051494 GO:0051495 GO:0051693 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0097435 GO:0110053 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04320 map04320

Pfam Domains

Protein Families

FH2

Protein Analysis

914

Amino Acids

100.03

Weight (kDa)

8.43

Isoelectric Point (pI)

57.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	458 - 862	4.5e-115	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011760)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43800
fragaria_vesca	FvH4_1g00460
malus_domestica	MD02G1003800.v1.1 MD15G1147100.v1.1
prunus_persica	Prupe.7G267500_v2.0.a1
pyrus_communis	pycom02g00230 pycom15g13200
rosa_chinensis	RchiOBHm_Chr2g0084971
rosa_laevigata	RLG00000015652
rosa_multiflora	Rmu_co8206718.1_g000001 Rmu_sc0017508.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155670
rosa_rugosa	Rorug01G0457400
rosa_samantha	Rh2AG005000 Rh2BG006200 Rh2BG300200 Rh2CG006800 Rh2DG006600
rosa_wichuraiana	Rw2G000500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1262
AccB1I	GGYRCC	2 cut(s)	1027, 2012
AccB7I	CCANNNNNTGG	1 cut(s)	1239
AccI	GTMKAC	2 cut(s)	1085, 1893
AccII	CGCG	1 cut(s)	1443
AccIII	TCCGGA	1 cut(s)	2547
AclWI	GGATC	3 cut(s)	1464, 1592, 1605
AcoI	YGGCCR	1 cut(s)	441
AcsI	RAATTY	2 cut(s)	150, 1808
AcuI	CTGAAG	5 cut(s)	958, 1233, 1835, 2078, 2154
AcyI	GRCGYC	2 cut(s)	194, 692
AfaI	GTAC	7 cut(s)	16, 526, 1833, 1868, 2115, 2301, 2701
AfeI	AGCGCT	1 cut(s)	1174
AfiI	CCNNNNNNNGG	8 cut(s)	439, 636, 1239, 1447, 1594, 1702, 2062, 2418
AjiI	CACGTC	1 cut(s)	337
AjnI	CCWGG	2 cut(s)	1232, 2689
AjuI	GAANNNNNNNTTGG	4 cut(s)	149, 181, 704, 736
AleI	CACNNNNGTG	1 cut(s)	2561
AloI	GAACNNNNNNTCC	2 cut(s)	764, 796
Alw21I	GWGCWC	5 cut(s)	516, 849, 1475, 1986, 2262
Alw26I	GTCTC	9 cut(s)	358, 364, 961, 1038, 1158, 1496, 1614, 1910, 2562
Alw44I	GTGCAC	1 cut(s)	512
AlwI	GGATC	3 cut(s)	1464, 1592, 1605
AlwNI	CAGNNNCTG	1 cut(s)	673
Ama87I	CYCGRG	1 cut(s)	2463
Aor13HI	TCCGGA	1 cut(s)	2547
Aor51HI	AGCGCT	1 cut(s)	1174
AoxI	GGCC	8 cut(s)	441, 453, 1037, 1391, 1428, 1668, 1795, 2227
ApaLI	GTGCAC	1 cut(s)	512
ApeKI	GCWGC	7 cut(s)	5, 371, 395, 520, 1928, 2279, 2707
ApoI	RAATTY	2 cut(s)	150, 1808
ArsI	GACNNNNNNTTYG	2 cut(s)	588, 620
AseI	ATTAAT	1 cut(s)	2363
Asp700I	GAANNNNTTC	2 cut(s)	2402, 2420
AspA2I	CCTAGG	1 cut(s)	1388
AspLEI	GCGC	3 cut(s)	1175, 1445, 1639
AspS9I	GGNCC	6 cut(s)	1274, 1325, 1428, 1669, 1796, 2693
AsuC2I	CCSGG	3 cut(s)	1066, 1300, 2201
AvaI	CYCGRG	1 cut(s)	2463
AvaII	GGWCC	3 cut(s)	1274, 1325, 2693
AvrII	CCTAGG	1 cut(s)	1388
BaeGI	GKGCMC	2 cut(s)	516, 2081
BamHI	GGATCC	1 cut(s)	1597
BanI	GGYRCC	2 cut(s)	1027, 2012
BanII	GRGCYC	4 cut(s)	680, 1306, 1475, 2262
BauI	CACGAG	1 cut(s)	613
BbsI	GAAGAC	2 cut(s)	780, 1068
Bbv12I	GWGCWC	5 cut(s)	516, 849, 1475, 1986, 2262
BbvI	GCAGC	7 cut(s)	17, 358, 382, 532, 1940, 2266, 2719
BccI	CCATC	7 cut(s)	133, 236, 302, 353, 1354, 1723, 2078
BceAI	ACGGC	3 cut(s)	335, 384, 646
BcgI	CGANNNNNNTGC	4 cut(s)	1990, 2015, 2024, 2049
BciT130I	CCWGG	2 cut(s)	1234, 2691
BclI	TGATCA	2 cut(s)	27, 1014
BcnI	CCSGG	3 cut(s)	1066, 1300, 2201
BcoDI	GTCTC	9 cut(s)	358, 364, 961, 1038, 1158, 1496, 1614, 1910, 2562
BfaI	CTAG	3 cut(s)	1157, 1389, 2585
BfmI	CTRYAG	2 cut(s)	855, 2628
BfoI	RGCGCY	2 cut(s)	1176, 1640
BfuAI	ACCTGC	1 cut(s)	1262
BglI	GCCNNNNNGGC	1 cut(s)	654
BlnI	CCTAGG	1 cut(s)	1388
BmcAI	AGTACT	1 cut(s)	16
Bme1390I	CCNGG	5 cut(s)	1066, 1234, 1300, 2201, 2691
Bme18I	GGWCC	3 cut(s)	1274, 1325, 2693
BmeT110I	CYCGRG	1 cut(s)	2463
BmgBI	CACGTC	1 cut(s)	337
BmgT120I	GGNCC	6 cut(s)	1274, 1325, 1428, 1669, 1796, 2693
BmiI	GGNNCC	9 cut(s)	754, 1029, 1305, 1326, 1599, 1734, 1997, 2014, 2719
BmrFI	CCNGG	5 cut(s)	1066, 1234, 1300, 2201, 2691
BmrI	ACTGGG	3 cut(s)	89, 758, 2001
BmsI	GCATC	3 cut(s)	911, 1846, 2017
BmuI	ACTGGG	3 cut(s)	89, 758, 2001
BpiI	GAAGAC	2 cut(s)	780, 1068
BpmI	CTGGAG	2 cut(s)	442, 915
Bpu10I	CCTNAGC	1 cut(s)	1179
BpuEI	CTTGAG	1 cut(s)	833
BpuMI	CCSGG	3 cut(s)	1066, 1300, 2201
BsaHI	GRCGYC	2 cut(s)	194, 692
BsaI	GGTCTC	1 cut(s)	364
BsaWI	WCCGGW	3 cut(s)	1051, 1258, 2547
BsaXI	ACNNNNNCTCC	6 cut(s)	229, 259, 275, 305, 440, 470
Bsc4I	CCNNNNNNNGG	8 cut(s)	439, 636, 1239, 1447, 1594, 1702, 2062, 2418
Bse118I	RCCGGY	1 cut(s)	648
Bse1I	ACTGG	6 cut(s)	95, 753, 932, 1328, 1996, 2681
Bse3DI	GCAATG	3 cut(s)	625, 1866, 1869
BseAI	TCCGGA	1 cut(s)	2547
BseBI	CCWGG	2 cut(s)	1234, 2691
BseGI	GGATG	8 cut(s)	179, 294, 1861, 2170, 2209, 2302, 2539, 2731
BseLI	CCNNNNNNNGG	8 cut(s)	439, 636, 1239, 1447, 1594, 1702, 2062, 2418
BseMI	GCAATG	3 cut(s)	625, 1866, 1869
BseMII	CTCAG	7 cut(s)	25, 849, 1449, 1625, 1794, 2166, 2294
BseNI	ACTGG	6 cut(s)	95, 753, 932, 1328, 1996, 2681
BseRI	GAGGAG	8 cut(s)	29, 65, 160, 175, 178, 237, 310, 1206
BseSI	GKGCMC	2 cut(s)	516, 2081
BseX3I	CGGCCG	1 cut(s)	441
BseXI	GCAGC	7 cut(s)	17, 358, 382, 532, 1940, 2266, 2719
BseYI	CCCAGC	3 cut(s)	673, 1798, 2327
BsgI	GTGCAG	2 cut(s)	357, 1136
Bsh1236I	CGCG	1 cut(s)	1443
Bsh1285I	CGRYCG	1 cut(s)	444
BshFI	GGCC	8 cut(s)	443, 455, 1039, 1393, 1430, 1670, 1797, 2229
BshNI	GGYRCC	2 cut(s)	1027, 2012
BsiEI	CGRYCG	1 cut(s)	444
BsiHKAI	GWGCWC	5 cut(s)	516, 849, 1475, 1986, 2262
BsiHKCI	CYCGRG	1 cut(s)	2463
BslFI	GGGAC	6 cut(s)	795, 963, 1048, 1267, 1338, 1430
BslI	CCNNNNNNNGG	8 cut(s)	439, 636, 1239, 1447, 1594, 1702, 2062, 2418
BsmAI	GTCTC	9 cut(s)	358, 364, 961, 1038, 1158, 1496, 1614, 1910, 2562
BsmBI	CGTCTC	2 cut(s)	358, 1038
BsmFI	GGGAC	6 cut(s)	795, 963, 1048, 1267, 1338, 1430
BsmI	GAATGC	2 cut(s)	1273, 2477
BsnI	GGCC	8 cut(s)	443, 455, 1039, 1393, 1430, 1670, 1797, 2229
Bso31I	GGTCTC	1 cut(s)	364
BsoBI	CYCGRG	1 cut(s)	2463
Bsp1286I	GDGCHC	8 cut(s)	516, 680, 849, 1306, 1475, 1986, 2081, 2262
Bsp13I	TCCGGA	1 cut(s)	2547
Bsp1407I	TGTACA	1 cut(s)	1866
Bsp143I	GATC	7 cut(s)	27, 1014, 1456, 1497, 1597, 2121, 2587
Bsp19I	CCATGG	1 cut(s)	2512
BspANI	GGCC	8 cut(s)	443, 455, 1039, 1393, 1430, 1670, 1797, 2229
BspCNI	CTCAG	7 cut(s)	24, 848, 1448, 1624, 1795, 2167, 2295
BspEI	TCCGGA	1 cut(s)	2547
BspFNI	CGCG	1 cut(s)	1443
BspHI	TCATGA	1 cut(s)	2661
BspLI	GGNNCC	9 cut(s)	754, 1029, 1305, 1326, 1599, 1734, 1997, 2014, 2719
BspMI	ACCTGC	1 cut(s)	1262
BspPI	GGATC	3 cut(s)	1464, 1592, 1605
BspQI	GCTCTTC	1 cut(s)	805
BspT107I	GGYRCC	2 cut(s)	1027, 2012
BspTNI	GGTCTC	1 cut(s)	364
BsrDI	GCAATG	3 cut(s)	625, 1866, 1869
BsrFI	RCCGGY	1 cut(s)	648
BsrGI	TGTACA	1 cut(s)	1866
BsrI	ACTGG	6 cut(s)	95, 753, 932, 1328, 1996, 2681
BssAI	RCCGGY	1 cut(s)	648
BssMI	GATC	7 cut(s)	27, 1014, 1456, 1497, 1597, 2121, 2587
BssNI	GRCGYC	2 cut(s)	194, 692
BssSI	CACGAG	1 cut(s)	613
BssT1I	CCWWGG	3 cut(s)	711, 1388, 2512
Bst2BI	CACGAG	1 cut(s)	613
Bst2UI	CCWGG	2 cut(s)	1234, 2691
Bst6I	CTCTTC	3 cut(s)	319, 805, 2436
BstACI	GRCGYC	2 cut(s)	194, 692
BstAPI	GCANNNNNTGC	1 cut(s)	2324
BstAUI	TGTACA	1 cut(s)	1866
BstC8I	GCNNGC	8 cut(s)	625, 659, 680, 832, 1041, 1432, 2231, 2596
BstDEI	CTNAG	8 cut(s)	11, 835, 1179, 1435, 1611, 1803, 2175, 2303
BstDSI	CCRYGG	2 cut(s)	2020, 2512
BstF5I	GGATG	8 cut(s)	179, 294, 1861, 2170, 2209, 2302, 2539, 2731
BstFNI	CGCG	1 cut(s)	1443
BstH2I	RGCGCY	2 cut(s)	1176, 1640
BstHHI	GCGC	3 cut(s)	1175, 1445, 1639
BstKTI	GATC	7 cut(s)	30, 1017, 1459, 1500, 1600, 2124, 2590
BstMAI	GTCTC	9 cut(s)	358, 364, 961, 1038, 1158, 1496, 1614, 1910, 2562
BstMBI	GATC	7 cut(s)	27, 1014, 1456, 1497, 1597, 2121, 2587
BstMCI	CGRYCG	1 cut(s)	444
BstNI	CCWGG	2 cut(s)	1234, 2691
BstNSI	RCATGY	1 cut(s)	2598
BstSCI	CCNGG	5 cut(s)	1064, 1232, 1298, 2199, 2689
BstSFI	CTRYAG	2 cut(s)	855, 2628
BstSLI	GKGCMC	2 cut(s)	516, 2081
BstUI	CGCG	1 cut(s)	1443
BstV1I	GCAGC	7 cut(s)	17, 358, 382, 532, 1940, 2266, 2719
BstV2I	GAAGAC	2 cut(s)	780, 1068
BstX2I	RGATCY	1 cut(s)	1597
BstYI	RGATCY	1 cut(s)	1597
BstZI	CGGCCG	1 cut(s)	441
BsuRI	GGCC	8 cut(s)	443, 455, 1039, 1393, 1430, 1670, 1797, 2229
BtgI	CCRYGG	2 cut(s)	2020, 2512
BtgZI	GCGATG	4 cut(s)	325, 331, 367, 2040
BtrI	CACGTC	1 cut(s)	337
BtsCI	GGATG	8 cut(s)	179, 294, 1861, 2170, 2209, 2302, 2539, 2731
BtsI	GCAGTG	2 cut(s)	1827, 1986
BtsIMutI	CAGTG	5 cut(s)	951, 1065, 1827, 1986, 2079
BveI	ACCTGC	1 cut(s)	1262
Cac8I	GCNNGC	8 cut(s)	625, 659, 680, 832, 1041, 1432, 2231, 2596
CaiI	CAGNNNCTG	1 cut(s)	673
CciI	TCATGA	1 cut(s)	2661
CfoI	GCGC	3 cut(s)	1175, 1445, 1639
Cfr10I	RCCGGY	1 cut(s)	648
Cfr13I	GGNCC	6 cut(s)	1274, 1325, 1428, 1669, 1796, 2693
CseI	GACGC	4 cut(s)	202, 592, 700, 1147
Csp6I	GTAC	7 cut(s)	15, 525, 1832, 1867, 2114, 2300, 2700
CviAII	CATG	9 cut(s)	584, 912, 1024, 2002, 2033, 2413, 2513, 2595, 2662
CviQI	GTAC	7 cut(s)	15, 525, 1832, 1867, 2114, 2300, 2700
DdeI	CTNAG	8 cut(s)	11, 835, 1179, 1435, 1611, 1803, 2175, 2303
DpnI	GATC	7 cut(s)	29, 1016, 1458, 1499, 1599, 2123, 2589
DpnII	GATC	7 cut(s)	27, 1014, 1456, 1497, 1597, 2121, 2587
EaeI	YGGCCR	1 cut(s)	441
EagI	CGGCCG	1 cut(s)	441
Eam1104I	CTCTTC	3 cut(s)	319, 805, 2436
EarI	CTCTTC	3 cut(s)	319, 805, 2436
EciI	GGCGGA	2 cut(s)	464, 1197
Ecl136II	GAGCTC	2 cut(s)	1473, 2260
EclXI	CGGCCG	1 cut(s)	441
Eco130I	CCWWGG	3 cut(s)	711, 1388, 2512
Eco147I	AGGCCT	1 cut(s)	455
Eco24I	GRGCYC	4 cut(s)	680, 1306, 1475, 2262
Eco31I	GGTCTC	1 cut(s)	364
Eco47I	GGWCC	3 cut(s)	1274, 1325, 2693
Eco47III	AGCGCT	1 cut(s)	1174
Eco52I	CGGCCG	1 cut(s)	441
Eco53kI	GAGCTC	2 cut(s)	1473, 2260
Eco57I	CTGAAG	5 cut(s)	958, 1233, 1835, 2078, 2154
Eco88I	CYCGRG	1 cut(s)	2463
EcoICRI	GAGCTC	2 cut(s)	1473, 2260
EcoO109I	RGGNCCY	1 cut(s)	1669
EcoRII	CCWGG	2 cut(s)	1232, 2689
EcoT14I	CCWWGG	3 cut(s)	711, 1388, 2512
EcoT22I	ATGCAT	1 cut(s)	1843
EcoT38I	GRGCYC	4 cut(s)	680, 1306, 1475, 2262
ErhI	CCWWGG	3 cut(s)	711, 1388, 2512
Esp3I	CGTCTC	2 cut(s)	358, 1038
FaeI	CATG	9 cut(s)	587, 915, 1027, 2005, 2036, 2416, 2516, 2598, 2665
FaqI	GGGAC	6 cut(s)	795, 963, 1048, 1267, 1338, 1430
FatI	CATG	9 cut(s)	583, 911, 1023, 2001, 2032, 2412, 2512, 2594, 2661
FauI	CCCGC	2 cut(s)	286, 492
FbaI	TGATCA	2 cut(s)	27, 1014
FblI	GTMKAC	2 cut(s)	1085, 1893
FokI	GGATG	8 cut(s)	166, 281, 1868, 2177, 2216, 2309, 2526, 2738
FriOI	GRGCYC	4 cut(s)	680, 1306, 1475, 2262
FspBI	CTAG	3 cut(s)	1157, 1389, 2585
GlaI	GCGC	3 cut(s)	1174, 1444, 1638
GsaI	CCCAGC	3 cut(s)	677, 1802, 2331
GsuI	CTGGAG	2 cut(s)	442, 915
HaeII	RGCGCY	2 cut(s)	1176, 1640
HaeIII	GGCC	8 cut(s)	443, 455, 1039, 1393, 1430, 1670, 1797, 2229
HgaI	GACGC	4 cut(s)	202, 592, 700, 1147
HhaI	GCGC	3 cut(s)	1175, 1445, 1639
Hin1I	GRCGYC	2 cut(s)	194, 692
Hin1II	CATG	9 cut(s)	587, 915, 1027, 2005, 2036, 2416, 2516, 2598, 2665
Hin6I	GCGC	3 cut(s)	1173, 1443, 1637
HinP1I	GCGC	3 cut(s)	1173, 1443, 1637
HincII	GTYRAC	1 cut(s)	2186
HindII	GTYRAC	1 cut(s)	2186
HindIII	AAGCTT	4 cut(s)	985, 1245, 1790, 1967
Hpy166II	GTNNAC	7 cut(s)	514, 1086, 1277, 1343, 1519, 1894, 2186
Hpy8I	GTNNAC	7 cut(s)	514, 1086, 1277, 1343, 1519, 1894, 2186
Hpy99I	CGWCG	9 cut(s)	196, 449, 509, 596, 608, 732, 761, 824, 1084
HpyCH4IV	ACGT	2 cut(s)	336, 819
HpyF3I	CTNAG	8 cut(s)	11, 835, 1179, 1435, 1611, 1803, 2175, 2303
HpySE526I	ACGT	2 cut(s)	336, 819
Hsp92I	GRCGYC	2 cut(s)	194, 692
Hsp92II	CATG	9 cut(s)	587, 915, 1027, 2005, 2036, 2416, 2516, 2598, 2665
HspAI	GCGC	3 cut(s)	1173, 1443, 1637
Kpn2I	TCCGGA	1 cut(s)	2547
Ksp22I	TGATCA	2 cut(s)	27, 1014
Kzo9I	GATC	7 cut(s)	27, 1014, 1456, 1497, 1597, 2121, 2587
LguI	GCTCTTC	1 cut(s)	805
Lsp1109I	GCAGC	7 cut(s)	17, 358, 382, 532, 1940, 2266, 2719
LweI	GCATC	3 cut(s)	911, 1846, 2017
MaeI	CTAG	3 cut(s)	1157, 1389, 2585
MaeII	ACGT	2 cut(s)	336, 819
MaeIII	GTNAC	6 cut(s)	62, 364, 496, 1054, 1288, 1645
MalI	GATC	7 cut(s)	29, 1016, 1458, 1499, 1599, 2123, 2589
MboI	GATC	7 cut(s)	27, 1014, 1456, 1497, 1597, 2121, 2587
MfeI	CAATTG	1 cut(s)	2655
MflI	RGATCY	1 cut(s)	1597
MhlI	GDGCHC	8 cut(s)	516, 680, 849, 1306, 1475, 1986, 2081, 2262
MlyI	GAGTC	4 cut(s)	817, 1001, 1249, 2402
MmeI	TCCRAC	7 cut(s)	137, 528, 600, 780, 972, 1760, 2693
Mph1103I	ATGCAT	1 cut(s)	1843
MroI	TCCGGA	1 cut(s)	2547
MroXI	GAANNNNTTC	2 cut(s)	2402, 2420
MseI	TTAA	8 cut(s)	1463, 1724, 1788, 1845, 1902, 1965, 2363, 2616
MslI	CAYNNNNRTG	2 cut(s)	910, 2561
MspA1I	CMGCKG	2 cut(s)	395, 1802
MspR9I	CCNGG	5 cut(s)	1066, 1234, 1300, 2201, 2691
MunI	CAATTG	1 cut(s)	2655
Mva1269I	GAATGC	2 cut(s)	1273, 2477
MvaI	CCWGG	2 cut(s)	1234, 2691
MvnI	CGCG	1 cut(s)	1443
NciI	CCSGG	3 cut(s)	1066, 1300, 2201
NcoI	CCATGG	1 cut(s)	2512
NdeII	GATC	7 cut(s)	27, 1014, 1456, 1497, 1597, 2121, 2587
NlaIII	CATG	9 cut(s)	587, 915, 1027, 2005, 2036, 2416, 2516, 2598, 2665
NlaIV	GGNNCC	9 cut(s)	754, 1029, 1305, 1326, 1599, 1734, 1997, 2014, 2719
NmeAIII	GCCGAG	5 cut(s)	419, 543, 1015, 1531, 2501
NmuCI	GTSAC	5 cut(s)	62, 364, 496, 1054, 1288
NsiI	ATGCAT	1 cut(s)	1843
NspI	RCATGY	1 cut(s)	2598
OliI	CACNNNNGTG	1 cut(s)	2561
PaeI	GCATGC	1 cut(s)	2598
PaeR7I	CTCGAG	1 cut(s)	2463
PagI	TCATGA	1 cut(s)	2661
PceI	AGGCCT	1 cut(s)	455
PciSI	GCTCTTC	1 cut(s)	805
PcsI	WCGNNNNNNNCGW	2 cut(s)	348, 600
PctI	GAATGC	2 cut(s)	1273, 2477
PdmI	GAANNNNTTC	2 cut(s)	2402, 2420
PflFI	GACNNNGTC	1 cut(s)	1060
PflMI	CCANNNNNTGG	1 cut(s)	1239
PfoI	TCCNGGA	2 cut(s)	2199, 2689
PleI	GAGTC	4 cut(s)	816, 1001, 1249, 2402
PpsI	GAGTC	4 cut(s)	816, 1001, 1249, 2402
PshBI	ATTAAT	1 cut(s)	2363
Psp124BI	GAGCTC	2 cut(s)	1475, 2262
Psp6I	CCWGG	2 cut(s)	1232, 2689
PspFI	CCCAGC	3 cut(s)	673, 1798, 2327
PspGI	CCWGG	2 cut(s)	1232, 2689
PspN4I	GGNNCC	9 cut(s)	754, 1029, 1305, 1326, 1599, 1734, 1997, 2014, 2719
PspPI	GGNCC	6 cut(s)	1274, 1325, 1428, 1669, 1796, 2693
PsrI	GAACNNNNNNTAC	2 cut(s)	542, 574
PstNI	CAGNNNCTG	1 cut(s)	673
PsuI	RGATCY	1 cut(s)	1597
PsyI	GACNNNGTC	1 cut(s)	1060
PvuII	CAGCTG	1 cut(s)	1802
RsaI	GTAC	7 cut(s)	16, 526, 1833, 1868, 2115, 2301, 2701
RsaNI	GTAC	7 cut(s)	15, 525, 1832, 1867, 2114, 2300, 2700
RseI	CAYNNNNRTG	2 cut(s)	910, 2561
SacI	GAGCTC	2 cut(s)	1475, 2262
SapI	GCTCTTC	1 cut(s)	805
SaqAI	TTAA	8 cut(s)	1463, 1724, 1788, 1845, 1902, 1965, 2363, 2616
Sau3AI	GATC	7 cut(s)	27, 1014, 1456, 1497, 1597, 2121, 2587
Sau96I	GGNCC	6 cut(s)	1274, 1325, 1428, 1669, 1796, 2693
ScaI	AGTACT	1 cut(s)	16
SchI	GAGTC	4 cut(s)	817, 1001, 1249, 2402
ScrFI	CCNGG	5 cut(s)	1066, 1234, 1300, 2201, 2691
SduI	GDGCHC	8 cut(s)	516, 680, 849, 1306, 1475, 1986, 2081, 2262
SfaNI	GCATC	3 cut(s)	911, 1846, 2017
SfcI	CTRYAG	2 cut(s)	855, 2628
Sfr274I	CTCGAG	1 cut(s)	2463
SinI	GGWCC	3 cut(s)	1274, 1325, 2693
SlaI	CTCGAG	1 cut(s)	2463
SmiMI	CAYNNNNRTG	2 cut(s)	910, 2561
SmlI	CTYRAG	2 cut(s)	848, 2463
SmoI	CTYRAG	2 cut(s)	848, 2463
SphI	GCATGC	1 cut(s)	2598
SseBI	AGGCCT	1 cut(s)	455
SspI	AATATT	2 cut(s)	1618, 2507
SspMI	CTAG	3 cut(s)	1157, 1389, 2585
SstI	GAGCTC	2 cut(s)	1475, 2262
StuI	AGGCCT	1 cut(s)	455
StyD4I	CCNGG	5 cut(s)	1064, 1232, 1298, 2199, 2689
StyI	CCWWGG	3 cut(s)	711, 1388, 2512
TaiI	ACGT	2 cut(s)	339, 822
TaqI	TCGA	8 cut(s)	191, 429, 995, 1469, 1500, 2046, 2406, 2464
TaqII	GACCGA	1 cut(s)	346
TatI	WGTACW	5 cut(s)	14, 1866, 2113, 2299, 2699
TauI	GCSGC	6 cut(s)	244, 395, 417, 420, 697, 1213
Tru1I	TTAA	8 cut(s)	1463, 1724, 1788, 1845, 1902, 1965, 2363, 2616
Tru9I	TTAA	8 cut(s)	1463, 1724, 1788, 1845, 1902, 1965, 2363, 2616
TscAI	CASTG	5 cut(s)	958, 1065, 1827, 1986, 2086
TseFI	GTSAC	5 cut(s)	62, 364, 496, 1054, 1288
TseI	GCWGC	7 cut(s)	5, 371, 395, 520, 1928, 2279, 2707
Tsp45I	GTSAC	5 cut(s)	62, 364, 496, 1054, 1288
TspGWI	ACGGA	4 cut(s)	352, 522, 745, 1718
TspRI	CASTG	5 cut(s)	958, 1065, 1827, 1986, 2086
Tth111I	GACNNNGTC	1 cut(s)	1060
Van91I	CCANNNNNTGG	1 cut(s)	1239
VneI	GTGCAC	1 cut(s)	512
VpaK11BI	GGWCC	3 cut(s)	1274, 1325, 2693
VspI	ATTAAT	1 cut(s)	2363
XapI	RAATTY	2 cut(s)	150, 1808
XbaI	TCTAGA	1 cut(s)	2584
XceI	RCATGY	1 cut(s)	2598
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1236
XhoI	CTCGAG	1 cut(s)	2463
XmaJI	CCTAGG	1 cut(s)	1388
XmiI	GTMKAC	2 cut(s)	1085, 1893
XmnI	GAANNNNTTC	2 cut(s)	2402, 2420
XspI	CTAG	3 cut(s)	1157, 1389, 2585
ZrmI	AGTACT	1 cut(s)	16
Zsp2I	ATGCAT	1 cut(s)	1843

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.