MD02G1005200.v1.1

Transcription elongation factor B polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005062
Physical Location & Seq
Forward (+)
360467 .. 362739
2273 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1005200.v1.1.491

Sequence Viewer

Length: 312 bp
ATGGCCATGTATATTCGTGTTAAGCGTAGTAAGACAACTTACTTTATCCAGTGTGAACCAACTGAAACAAGTTTAGATATAAAGCAGAAATTACATGATCTTATTGATCGACCGGTAAATGACCAGCGCTTGATCTTAGTCAGTACTGGGGAAGTATTGGAGGATTCAAAGGCGTTGGCGGATCAGAAGGTTGAAAATGACGCCGTTGTTGCACTGGCTTTCAGAAAAGATGACAATGAGTTTGAAGAGGTCAACATTGTTCGGCCAGACGACTTCTATCCGTCTCGTGATGCAGATGCAGGCAGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04066 ko04120 ko05200 ko05211 map04066 map04120 map05200 map05211
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

104

Amino Acids

11.85

Weight (kDa)

4.62

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 5 - 76 8.8e-11 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 179
AclWI GGATC 1 cut(s) 189
AcoI YGGCCR 2 cut(s) 3, 263
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 1 cut(s) 145
AfeI AGCGCT 1 cut(s) 128
AgeI ACCGGT 1 cut(s) 112
AgsI TTSAA 3 cut(s) 168, 194, 245
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 1 cut(s) 189
Aor51HI AGCGCT 1 cut(s) 128
AoxI GGCC 2 cut(s) 3, 263
ArsI GACNNNNNNTTYG 2 cut(s) 224, 256
AsiGI ACCGGT 1 cut(s) 112
AspLEI GCGC 1 cut(s) 129
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 285
BceAI ACGGC 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 288
BfoI RGCGCY 1 cut(s) 130
BmcAI AGTACT 1 cut(s) 145
BmrI ACTGGG 1 cut(s) 156
BmsI GCATC 2 cut(s) 280, 286
BmuI ACTGGG 1 cut(s) 156
BsaHI GRCGYC 1 cut(s) 201
BsaWI WCCGGW 1 cut(s) 112
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 3 cut(s) 49, 151, 219
BseNI ACTGG 3 cut(s) 49, 151, 219
Bsh1285I CGRYCG 1 cut(s) 113
BshFI GGCC 2 cut(s) 5, 265
BshTI ACCGGT 1 cut(s) 112
BsiEI CGRYCG 1 cut(s) 113
BsiSI CCGG 1 cut(s) 113
BsmAI GTCTC 1 cut(s) 288
BsmBI CGTCTC 1 cut(s) 288
BsnI GGCC 2 cut(s) 5, 265
Bsp143I GATC 4 cut(s) 97, 106, 132, 181
BspACI CCGC 1 cut(s) 179
BspANI GGCC 2 cut(s) 5, 265
BspPI GGATC 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 112
BsrI ACTGG 3 cut(s) 49, 151, 219
BssAI RCCGGY 1 cut(s) 112
BssMI GATC 4 cut(s) 97, 106, 132, 181
BssNI GRCGYC 1 cut(s) 201
BssSI CACGAG 1 cut(s) 285
Bst2BI CACGAG 1 cut(s) 285
Bst6I CTCTTC 1 cut(s) 240
BstACI GRCGYC 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 1 cut(s) 136
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 4 cut(s) 100, 109, 135, 184
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 4 cut(s) 97, 106, 132, 181
BstMCI CGRYCG 1 cut(s) 113
BstMWI GCNNNNNNNGC 1 cut(s) 209
BsuRI GGCC 2 cut(s) 5, 265
BtsIMutI CAGTG 2 cut(s) 56, 212
Cac8I GCNNGC 1 cut(s) 301
CfoI GCGC 1 cut(s) 129
Cfr10I RCCGGY 1 cut(s) 112
CseI GACGC 1 cut(s) 209
Csp6I GTAC 1 cut(s) 144
CspAI ACCGGT 1 cut(s) 112
CviAII CATG 2 cut(s) 7, 95
CviJI RGCY 3 cut(s) 5, 218, 265
CviKI_1 RGCY 3 cut(s) 5, 218, 265
CviQI GTAC 1 cut(s) 144
DdeI CTNAG 1 cut(s) 136
DpnI GATC 4 cut(s) 99, 108, 134, 183
DpnII GATC 4 cut(s) 97, 106, 132, 181
EaeI YGGCCR 2 cut(s) 3, 263
Eam1104I CTCTTC 1 cut(s) 240
EarI CTCTTC 1 cut(s) 240
EciI GGCGGA 1 cut(s) 194
Eco47III AGCGCT 1 cut(s) 128
Esp3I CGTCTC 1 cut(s) 288
FaeI CATG 2 cut(s) 10, 98
FaiI YATR 4 cut(s) 8, 12, 80, 96
FatI CATG 2 cut(s) 6, 94
GlaI GCGC 1 cut(s) 128
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 2 cut(s) 5, 265
HapII CCGG 1 cut(s) 113
HgaI GACGC 1 cut(s) 209
HhaI GCGC 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 2 cut(s) 10, 98
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 1 cut(s) 164
HpaII CCGG 1 cut(s) 113
Hpy166II GTNNAC 2 cut(s) 56, 253
Hpy188I TCNGA 2 cut(s) 186, 224
Hpy188III TCNNGA 1 cut(s) 287
Hpy8I GTNNAC 2 cut(s) 56, 253
HpyAV CCTTC 1 cut(s) 181
HpyCH4V TGCA 3 cut(s) 212, 293, 299
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 1 cut(s) 136
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 2 cut(s) 10, 98
HspAI GCGC 1 cut(s) 127
Kzo9I GATC 4 cut(s) 97, 106, 132, 181
LpnPI CCDG 7 cut(s) 62, 126, 132, 137, 200, 279, 285
LweI GCATC 2 cut(s) 280, 286
MalI GATC 4 cut(s) 99, 108, 134, 183
MboI GATC 4 cut(s) 97, 106, 132, 181
MboII GAAGA 1 cut(s) 257
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 89
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 2 cut(s) 154, 241
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 21
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 209
NdeII GATC 4 cut(s) 97, 106, 132, 181
NlaIII CATG 2 cut(s) 10, 98
PcsI WCGNNNNNNNCGW 1 cut(s) 22
PfeI GAWTC 1 cut(s) 164
PinAI ACCGGT 1 cut(s) 112
RsaI GTAC 1 cut(s) 145
RsaNI GTAC 1 cut(s) 144
SaqAI TTAA 1 cut(s) 21
Sau3AI GATC 4 cut(s) 97, 106, 132, 181
ScaI AGTACT 1 cut(s) 145
SetI ASST 2 cut(s) 192, 252
SfaNI GCATC 2 cut(s) 280, 286
Sse9I AATT 1 cut(s) 89
SsiI CCGC 1 cut(s) 179
TaqI TCGA 1 cut(s) 109
TasI AATT 1 cut(s) 89
TatI WGTACW 1 cut(s) 143
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TscAI CASTG 2 cut(s) 56, 219
TspGWI ACGGA 1 cut(s) 270
TspRI CASTG 2 cut(s) 56, 219
ZrmI AGTACT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.