Rroxscaffold_2G00155510

Transcription elongation factor B polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005062
Physical Location & Seq
Reverse (-)
91913799 .. 91916700
2902 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00155510.1

Sequence Viewer

Length: 360 bp
ATGGCCATGTATATCCGTGTTAAGCGTAGTAAGACAACTTACTTTATCCAGTGTGAACCAACCGAGACAATTTTAGATATTAAGCAGAAATTGCAAAATCTTATTGATCAACCGGTAAATGATCAGCGTTTGATCCTAGTCAGTACTGGGGAAGTACTGGAAGATTCAAAGACATTGGCAGATCAGAAGGTGGAAAATGATGGCGTTGTAGCACTGACTTTGAGAAAAGGTCGAGTCTCTTATGCTTTTGTTCTTTGGCCCTCGACTTTTATGCATCATGATGATGAGTTTGAAGAAGTGAACATTGTACGACCAGATGACTTCTACCAATCTCGTGATGCAGATGCAAGCAGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04066 ko04120 ko05200 ko05211 map04066 map04120 map05200 map05211
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

119

Amino Acids

13.81

Weight (kDa)

4.83

Isoelectric Point (pI)

59.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 5 - 76 3.9e-12 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 127
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 3 cut(s) 145, 156, 309
AgeI ACCGGT 1 cut(s) 112
AgsI TTSAA 2 cut(s) 168, 293
Alw26I GTCTC 2 cut(s) 59, 241
AlwI GGATC 1 cut(s) 127
AoxI GGCC 2 cut(s) 3, 257
AsiGI ACCGGT 1 cut(s) 112
AspS9I GGNCC 1 cut(s) 258
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 333
BccI CCATC 1 cut(s) 194
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BclI TGATCA 2 cut(s) 106, 121
BcoDI GTCTC 2 cut(s) 59, 241
BfaI CTAG 1 cut(s) 137
BmcAI AGTACT 2 cut(s) 145, 156
BmgT120I GGNCC 1 cut(s) 258
BmrI ACTGGG 1 cut(s) 156
BmsI GCATC 3 cut(s) 283, 328, 334
BmuI ACTGGG 1 cut(s) 156
BsaWI WCCGGW 1 cut(s) 112
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 3 cut(s) 49, 151, 162
BseNI ACTGG 3 cut(s) 49, 151, 162
BshFI GGCC 2 cut(s) 5, 259
BshTI ACCGGT 1 cut(s) 112
BsiSI CCGG 1 cut(s) 113
BsmAI GTCTC 2 cut(s) 59, 241
BsnI GGCC 2 cut(s) 5, 259
Bsp143I GATC 4 cut(s) 106, 121, 132, 181
BspANI GGCC 2 cut(s) 5, 259
BspHI TCATGA 1 cut(s) 277
BspPI GGATC 1 cut(s) 127
BsrFI RCCGGY 1 cut(s) 112
BsrI ACTGG 3 cut(s) 49, 151, 162
BssAI RCCGGY 1 cut(s) 112
BssMI GATC 4 cut(s) 106, 121, 132, 181
BssSI CACGAG 1 cut(s) 333
Bst2BI CACGAG 1 cut(s) 333
BstAPI GCANNNNNTGC 1 cut(s) 91
BstC8I GCNNGC 1 cut(s) 349
BstKTI GATC 4 cut(s) 109, 124, 135, 184
BstMAI GTCTC 2 cut(s) 59, 241
BstMBI GATC 4 cut(s) 106, 121, 132, 181
BstMWI GCNNNNNNNGC 1 cut(s) 91
BsuRI GGCC 2 cut(s) 5, 259
BtsIMutI CAGTG 2 cut(s) 56, 212
Cac8I GCNNGC 1 cut(s) 349
CciI TCATGA 1 cut(s) 277
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 1 cut(s) 258
Csp6I GTAC 3 cut(s) 144, 155, 308
CspAI ACCGGT 1 cut(s) 112
CviAII CATG 2 cut(s) 7, 278
CviJI RGCY 2 cut(s) 5, 259
CviKI_1 RGCY 2 cut(s) 5, 259
CviQI GTAC 3 cut(s) 144, 155, 308
DpnI GATC 4 cut(s) 108, 123, 134, 183
DpnII GATC 4 cut(s) 106, 121, 132, 181
EaeI YGGCCR 1 cut(s) 3
EcoT22I ATGCAT 1 cut(s) 276
FaeI CATG 2 cut(s) 10, 281
FaiI YATR 5 cut(s) 8, 12, 243, 272, 279
FatI CATG 2 cut(s) 6, 277
FbaI TGATCA 2 cut(s) 106, 121
FspBI CTAG 1 cut(s) 137
HaeIII GGCC 2 cut(s) 5, 259
HapII CCGG 1 cut(s) 113
Hin1II CATG 2 cut(s) 10, 281
HinfI GANTC 2 cut(s) 164, 234
HpaII CCGG 1 cut(s) 113
Hpy166II GTNNAC 2 cut(s) 56, 301
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 2 cut(s) 278, 335
Hpy8I GTNNAC 2 cut(s) 56, 301
HpyAV CCTTC 1 cut(s) 181
HpyCH4V TGCA 4 cut(s) 94, 274, 341, 347
HpyF10VI GCNNNNNNNGC 1 cut(s) 91
Hsp92II CATG 2 cut(s) 10, 281
Ksp22I TGATCA 2 cut(s) 106, 121
Kzo9I GATC 4 cut(s) 106, 121, 132, 181
LpnPI CCDG 5 cut(s) 62, 126, 132, 143, 327
LweI GCATC 3 cut(s) 283, 328, 334
MaeI CTAG 1 cut(s) 137
MalI GATC 4 cut(s) 108, 123, 134, 183
MboI GATC 4 cut(s) 106, 121, 132, 181
MboII GAAGA 2 cut(s) 173, 305
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 69, 89
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 243
MnlI CCTC 1 cut(s) 271
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 276
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 21, 81
MslI CAYNNNNRTG 2 cut(s) 279, 282
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 91
NdeII GATC 4 cut(s) 106, 121, 132, 181
NlaIII CATG 2 cut(s) 10, 281
NsiI ATGCAT 1 cut(s) 276
PagI TCATGA 1 cut(s) 277
PfeI GAWTC 1 cut(s) 164
PinAI ACCGGT 1 cut(s) 112
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
PspPI GGNCC 1 cut(s) 258
RsaI GTAC 3 cut(s) 145, 156, 309
RsaNI GTAC 3 cut(s) 144, 155, 308
RseI CAYNNNNRTG 2 cut(s) 279, 282
SaqAI TTAA 2 cut(s) 21, 81
Sau3AI GATC 4 cut(s) 106, 121, 132, 181
Sau96I GGNCC 1 cut(s) 258
ScaI AGTACT 2 cut(s) 145, 156
SchI GAGTC 1 cut(s) 243
SetI ASST 2 cut(s) 192, 232
SfaNI GCATC 3 cut(s) 283, 328, 334
SmiMI CAYNNNNRTG 2 cut(s) 279, 282
Sse9I AATT 2 cut(s) 69, 89
SspMI CTAG 1 cut(s) 137
TaqI TCGA 2 cut(s) 232, 263
TasI AATT 2 cut(s) 69, 89
TatI WGTACW 2 cut(s) 143, 154
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 2 cut(s) 21, 81
Tru9I TTAA 2 cut(s) 21, 81
TscAI CASTG 2 cut(s) 56, 219
TspGWI ACGGA 1 cut(s) 5
TspRI CASTG 2 cut(s) 56, 219
XspI CTAG 1 cut(s) 137
ZrmI AGTACT 2 cut(s) 145, 156
Zsp2I ATGCAT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.