MD02G1009300.v1.1

Belongs to the aldehyde dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007245
Physical Location & Seq
Reverse (-)
621574 .. 625348
3775 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1009300.v1.1.491

Sequence Viewer

Length: 1491 bp
ATGGCTGGGAGTGGATTGTTTGCAGAGATTGTTGATGGAGAAGCATACAAGTATTATGCTGATGGGGAGTGGAAGAAATCATCTTCTGGTAAACTTGTTCCCATCATCAACCCTACCACTAGAAAGGTCCACTACAAGGTTCAAGCTTGTACACAGGAAGAGGTTAACAAGGTCATGGAGACCGCGAAAATCGCACAGAAGACATGGGCTAAGACCCCGCTTTGGAAGCGGGCCGAGCTCCTTCACAAGGCTGCTTCTATCCTCAAAGAACACAAAGCTCCAATTGGAGAGTCACTGATTAAAGAAATTGCTAAACCTGCTAAAGATGCTATCACAGAGGTTGTGAGGTCTGGAGATTTGGTGTCTTACTGTGCCGAAGAAGGGGTTAGGATTCTCGGGGAAGGGAAGTTCTTGGTGTCTGATAGTTTCCCGGGAAATGACAGAACAAAATATTGCTTAACTTCCAAGATTCCACTCGGCGTGGTTCTAGCCATTCCACCATTTAACTATCCTGTCAACCTTGCTGTCTCAAAAATTGCTCCTGCGCTGATCGCTGGAAACGCCCTTGTGCTCAAGCCCCCAACTCAGGGTGCTGTTTCTTGCCTTCATATGGTGCATTGCTTCCACCTGGCTGGATTTCCCAAAGGCCTTATTAGCTGTGTCACTGGGAAAGGATCTGAGATCGGCGACTTCCTTACCATGCATCCTGGAGTGGACTGCATTAGCTTCACTGGTGGTGACACCGGCGTTGCAATTTCGAAGAAGGCAGGCATGATCCCTCTTCAGATGGAGTTGGGAGGAAAAGATGCATGCATTGTGCTTGAAGATGCCGACCTAGATTTGGTCGCGGCAAACATAATAAAGGGAGGCTTTTCTTACAGTGGTCAAAGATGTACTGCTGTTAAGGTTGTCTTGGTGATGGAATCCGTTGCTGATGCCCTCGTCGCGAAAGTGAATGCACGAGTGGCGAAATTAACTGTTGGTCCACCAGAGGAGAACAGTGACATCACTCCCGTAGTCTCAGAATCATCTGCGAATTTTATTGAAGGTTTGGTTGTGGATGCAAAACAGAAAGGAGCAACATTTTGCCAAGAGTACAAGAGAGAGGGCAACCTTATCTGGCCTTTGTTGTTAGATAATGTCAGGCCGGATATGAGAATCGCGTGGGAAGAGCCATTCGGTCCAGTCTTGCCCGTTATTAGGATTACTACGGTAGAAGAAGGAATCCACCATTGCAATGCCAGTAATTTCGGCCTCCAGGGCTGTGTGTTCACAAAGGACATCAACAAGGCCATTTTGATTGGTGATGCAATGGAGACGGGAACTGTTCAAATAAACTCCGCACCTGCTCGTGGACCAGATCATTTTCCGTTCCAGGGCATAAAGGACAGTGGAATTGGCTCACAAGGAATCACCAACAGCATCAACATGATGACCAAGATCAAGACCACTGTAATCAACTTGCCAACCCCTTCTTACACCATGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko01100 ko01120 ko01200 map00010 map00030 map01100 map01120 map01200
Pfam Domains
Protein Families
Aldedh

Protein Analysis

497

Amino Acids

53.08

Weight (kDa)

7.11

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldedh PF00171 24 - 483 6e-143 Aldehyde dehydrogenase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1354
Acc36I ACCTGC 2 cut(s) 325, 1354
AccII CGCG 4 cut(s) 185, 848, 947, 1163
AciI CCGC 5 cut(s) 183, 218, 229, 848, 1341
AclWI GGATC 2 cut(s) 682, 769
AcsI RAATTY 1 cut(s) 1036
AcuI CTGAAG 1 cut(s) 767
AfaI GTAC 3 cut(s) 151, 895, 1097
AgsI TTSAA 4 cut(s) 143, 824, 1046, 1331
AjnI CCWGG 4 cut(s) 627, 706, 1257, 1374
AloI GAACNNNNNNTCC 2 cut(s) 392, 424
AluBI AGCT 5 cut(s) 146, 238, 278, 657, 726
AluI AGCT 5 cut(s) 146, 238, 278, 657, 726
Alw21I GWGCWC 2 cut(s) 240, 573
Alw26I GTCTC 4 cut(s) 173, 532, 1024, 1310
AlwI GGATC 2 cut(s) 682, 769
Ama87I CYCGRG 2 cut(s) 395, 430
AoxI GGCC 6 cut(s) 231, 646, 1121, 1145, 1252, 1290
ApeKI GCWGC 1 cut(s) 251
ApoI RAATTY 1 cut(s) 1036
AspLEI GCGC 1 cut(s) 547
AspS9I GGNCC 5 cut(s) 127, 231, 983, 1181, 1355
AsuC2I CCSGG 2 cut(s) 431, 432
AsuHPI GGTGA 4 cut(s) 749, 928, 1316, 1405
AsuII TTCGAA 1 cut(s) 758
AvaI CYCGRG 2 cut(s) 395, 430
AvaII GGWCC 4 cut(s) 127, 983, 1181, 1355
BanII GRGCYC 1 cut(s) 240
BauI CACGAG 2 cut(s) 960, 1350
BbsI GAAGAC 1 cut(s) 206
Bbv12I GWGCWC 2 cut(s) 240, 573
BbvI GCAGC 1 cut(s) 238
BccI CCATC 5 cut(s) 29, 56, 110, 781, 913
BciT130I CCWGG 4 cut(s) 629, 708, 1259, 1376
BcnI CCSGG 2 cut(s) 431, 432
BcoDI GTCTC 4 cut(s) 173, 532, 1024, 1310
BfaI CTAG 3 cut(s) 120, 488, 836
BfuAI ACCTGC 2 cut(s) 325, 1354
BglI GCCNNNNNGGC 1 cut(s) 1260
BisI GCNGC 2 cut(s) 252, 849
BlsI GCNGC 2 cut(s) 253, 850
Bme1390I CCNGG 6 cut(s) 431, 432, 629, 708, 1259, 1376
Bme18I GGWCC 4 cut(s) 127, 983, 1181, 1355
BmeT110I CYCGRG 2 cut(s) 395, 430
BmgT120I GGNCC 5 cut(s) 127, 231, 983, 1181, 1355
BmrFI CCNGG 6 cut(s) 431, 432, 629, 708, 1259, 1376
BmrI ACTGGG 1 cut(s) 675
BmsI GCATC 8 cut(s) 316, 712, 796, 817, 925, 1051, 1297, 1431
BmuI ACTGGG 1 cut(s) 675
BpiI GAAGAC 1 cut(s) 206
BpmI CTGGAG 3 cut(s) 372, 729, 1241
Bpu14I TTCGAA 1 cut(s) 758
BpuEI CTTGAG 1 cut(s) 557
BpuMI CCSGG 2 cut(s) 431, 432
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 4 cut(s) 430, 1258, 1375, 1482
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bse118I RCCGGY 1 cut(s) 743
Bse1I ACTGG 4 cut(s) 670, 736, 1184, 1242
Bse3DI GCAATG 4 cut(s) 616, 1231, 1243, 1317
BseBI CCWGG 4 cut(s) 629, 708, 1259, 1376
BseDI CCNNGG 4 cut(s) 430, 1258, 1375, 1482
BseGI GGATG 2 cut(s) 703, 1066
BseMI GCAATG 4 cut(s) 616, 1231, 1243, 1317
BseMII CTCAG 3 cut(s) 599, 669, 1035
BseNI ACTGG 4 cut(s) 670, 736, 1184, 1242
BseRI GAGGAG 1 cut(s) 1007
BseXI GCAGC 1 cut(s) 238
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 4 cut(s) 185, 848, 947, 1163
BshFI GGCC 6 cut(s) 233, 648, 1123, 1147, 1254, 1292
BsiHKAI GWGCWC 2 cut(s) 240, 573
BsiHKCI CYCGRG 2 cut(s) 395, 430
BsiSI CCGG 3 cut(s) 431, 744, 1148
BsmAI GTCTC 4 cut(s) 173, 532, 1024, 1310
BsmBI CGTCTC 1 cut(s) 1310
BsmI GAATGC 1 cut(s) 961
BsnI GGCC 6 cut(s) 233, 648, 1123, 1147, 1254, 1292
Bso31I GGTCTC 1 cut(s) 173
BsoBI CYCGRG 2 cut(s) 395, 430
Bsp119I TTCGAA 1 cut(s) 758
Bsp1286I GDGCHC 2 cut(s) 240, 573
Bsp1407I TGTACA 1 cut(s) 149
Bsp143I GATC 6 cut(s) 549, 674, 681, 774, 1360, 1440
Bsp19I CCATGG 1 cut(s) 1482
Bsp68I TCGCGA 1 cut(s) 947
BspACI CCGC 5 cut(s) 183, 218, 229, 848, 1341
BspANI GGCC 6 cut(s) 233, 648, 1123, 1147, 1254, 1292
BspCNI CTCAG 3 cut(s) 598, 670, 1034
BspFNI CGCG 4 cut(s) 185, 848, 947, 1163
BspMI ACCTGC 2 cut(s) 325, 1354
BspPI GGATC 2 cut(s) 682, 769
BspQI GCTCTTC 1 cut(s) 1164
BspT104I TTCGAA 1 cut(s) 758
BspTNI GGTCTC 1 cut(s) 173
BsrDI GCAATG 4 cut(s) 616, 1231, 1243, 1317
BsrFI RCCGGY 1 cut(s) 743
BsrGI TGTACA 1 cut(s) 149
BsrI ACTGG 4 cut(s) 670, 736, 1184, 1242
BssAI RCCGGY 1 cut(s) 743
BssECI CCNNGG 4 cut(s) 430, 1258, 1375, 1482
BssMI GATC 6 cut(s) 549, 674, 681, 774, 1360, 1440
BssSI CACGAG 2 cut(s) 960, 1350
BssT1I CCWWGG 1 cut(s) 1482
Bst2BI CACGAG 2 cut(s) 960, 1350
Bst2UI CCWGG 4 cut(s) 629, 708, 1259, 1376
Bst4CI ACNGT 8 cut(s) 371, 881, 979, 1001, 1213, 1327, 1391, 1453
Bst6I CTCTTC 3 cut(s) 153, 786, 1164
BstAUI TGTACA 1 cut(s) 149
BstBI TTCGAA 1 cut(s) 758
BstC8I GCNNGC 3 cut(s) 231, 769, 811
BstDEI CTNAG 4 cut(s) 210, 585, 678, 1021
BstDSI CCRYGG 1 cut(s) 1482
BstENI CCTNNNNNAGG 1 cut(s) 245
BstF5I GGATG 2 cut(s) 703, 1066
BstFNI CGCG 4 cut(s) 185, 848, 947, 1163
BstHHI GCGC 1 cut(s) 547
BstKTI GATC 6 cut(s) 552, 677, 684, 777, 1363, 1443
BstMAI GTCTC 4 cut(s) 173, 532, 1024, 1310
BstMBI GATC 6 cut(s) 549, 674, 681, 774, 1360, 1440
BstNI CCWGG 4 cut(s) 629, 708, 1259, 1376
BstNSI RCATGY 1 cut(s) 813
BstSCI CCNGG 6 cut(s) 429, 430, 627, 706, 1257, 1374
BstUI CGCG 4 cut(s) 185, 848, 947, 1163
BstV1I GCAGC 1 cut(s) 238
BstV2I GAAGAC 1 cut(s) 206
BstX2I RGATCY 1 cut(s) 674
BstXI CCANNNNNNTGG 1 cut(s) 632
BstYI RGATCY 1 cut(s) 674
BsuRI GGCC 6 cut(s) 233, 648, 1123, 1147, 1254, 1292
BtgI CCRYGG 1 cut(s) 1482
BtsCI GGATG 2 cut(s) 703, 1066
BtsIMutI CAGTG 7 cut(s) 293, 663, 729, 886, 1006, 1396, 1449
BtuMI TCGCGA 1 cut(s) 947
BveI ACCTGC 2 cut(s) 325, 1354
Cac8I GCNNGC 3 cut(s) 231, 769, 811
CfoI GCGC 1 cut(s) 547
Cfr10I RCCGGY 1 cut(s) 743
Cfr13I GGNCC 5 cut(s) 127, 231, 983, 1181, 1355
Cfr9I CCCGGG 1 cut(s) 430
Csp6I GTAC 3 cut(s) 150, 894, 1096
CviAII CATG 7 cut(s) 175, 204, 700, 772, 810, 1429, 1483
CviQI GTAC 3 cut(s) 150, 894, 1096
DdeI CTNAG 4 cut(s) 210, 585, 678, 1021
DpnI GATC 6 cut(s) 551, 676, 683, 776, 1362, 1442
DpnII GATC 6 cut(s) 549, 674, 681, 774, 1360, 1440
Eam1104I CTCTTC 3 cut(s) 153, 786, 1164
EarI CTCTTC 3 cut(s) 153, 786, 1164
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 1482
Eco147I AGGCCT 1 cut(s) 648
Eco24I GRGCYC 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 4 cut(s) 127, 983, 1181, 1355
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 767
Eco88I CYCGRG 2 cut(s) 395, 430
EcoICRI GAGCTC 1 cut(s) 238
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoRII CCWGG 4 cut(s) 627, 706, 1257, 1374
EcoT14I CCWWGG 1 cut(s) 1482
EcoT22I ATGCAT 3 cut(s) 705, 811, 815
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 1482
Esp3I CGTCTC 1 cut(s) 1310
FaeI CATG 7 cut(s) 178, 207, 703, 775, 813, 1432, 1486
FalI AAGNNNNNCTT 4 cut(s) 854, 886, 896, 928
FatI CATG 7 cut(s) 174, 203, 699, 771, 809, 1428, 1482
FauI CCCGC 2 cut(s) 222, 225
FauNDI CATATG 1 cut(s) 609
Fnu4HI GCNGC 2 cut(s) 252, 849
FokI GGATG 2 cut(s) 690, 1073
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 2 cut(s) 252, 849
FspBI CTAG 3 cut(s) 120, 488, 836
GlaI GCGC 1 cut(s) 546
GluI GCNGC 2 cut(s) 252, 849
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 3 cut(s) 372, 729, 1241
HaeIII GGCC 6 cut(s) 233, 648, 1123, 1147, 1254, 1292
HapII CCGG 3 cut(s) 431, 744, 1148
HhaI GCGC 1 cut(s) 547
Hin1II CATG 7 cut(s) 178, 207, 703, 775, 813, 1432, 1486
Hin6I GCGC 1 cut(s) 545
HinP1I GCGC 1 cut(s) 545
HincII GTYRAC 2 cut(s) 166, 517
HindII GTYRAC 2 cut(s) 166, 517
HindIII AAGCTT 1 cut(s) 144
HinfI GANTC 8 cut(s) 290, 391, 469, 923, 1025, 1158, 1224, 1410
HpaI GTTAAC 1 cut(s) 166
HpaII CCGG 3 cut(s) 431, 744, 1148
HphI GGTGA 4 cut(s) 749, 928, 1316, 1405
Hpy166II GTNNAC 9 cut(s) 92, 130, 152, 166, 517, 715, 986, 1272, 1355
Hpy188I TCNGA 4 cut(s) 421, 679, 786, 1024
Hpy188III TCNNGA 3 cut(s) 351, 946, 1444
Hpy8I GTNNAC 9 cut(s) 92, 130, 152, 166, 517, 715, 986, 1272, 1355
Hpy99I CGWCG 1 cut(s) 947
HpyAV CCTTC 8 cut(s) 251, 374, 395, 614, 757, 1040, 1214, 1482
HpyCH4III ACNGT 8 cut(s) 371, 881, 979, 1001, 1213, 1327, 1391, 1453
HpyF3I CTNAG 4 cut(s) 210, 585, 678, 1021
Hsp92II CATG 7 cut(s) 178, 207, 703, 775, 813, 1432, 1486
HspAI GCGC 1 cut(s) 545
KspAI GTTAAC 1 cut(s) 166
Kzo9I GATC 6 cut(s) 549, 674, 681, 774, 1360, 1440
LguI GCTCTTC 1 cut(s) 1164
LmnI GCTCC 4 cut(s) 243, 283, 544, 1076
Lsp1109I GCAGC 1 cut(s) 238
LweI GCATC 8 cut(s) 316, 712, 796, 817, 925, 1051, 1297, 1431
MaeI CTAG 3 cut(s) 120, 488, 836
MaeIII GTNAC 4 cut(s) 291, 661, 737, 1001
MalI GATC 6 cut(s) 551, 676, 683, 776, 1362, 1442
MboI GATC 6 cut(s) 549, 674, 681, 774, 1360, 1440
MfeI CAATTG 1 cut(s) 282
MflI RGATCY 1 cut(s) 674
MhlI GDGCHC 2 cut(s) 240, 573
MluCI AATT 8 cut(s) 282, 306, 534, 753, 971, 1036, 1246, 1395
MlyI GAGTC 1 cut(s) 299
Mph1103I ATGCAT 3 cut(s) 705, 811, 815
MseI TTAA 6 cut(s) 165, 300, 458, 504, 903, 974
MslI CAYNNNNRTG 2 cut(s) 1236, 1427
MspI CCGG 3 cut(s) 431, 744, 1148
MspR9I CCNGG 6 cut(s) 431, 432, 629, 708, 1259, 1376
MunI CAATTG 1 cut(s) 282
Mva1269I GAATGC 1 cut(s) 961
MvaI CCWGG 4 cut(s) 629, 708, 1259, 1376
MvnI CGCG 4 cut(s) 185, 848, 947, 1163
NciI CCSGG 2 cut(s) 431, 432
NcoI CCATGG 1 cut(s) 1482
NdeI CATATG 1 cut(s) 609
NdeII GATC 6 cut(s) 549, 674, 681, 774, 1360, 1440
NlaIII CATG 7 cut(s) 178, 207, 703, 775, 813, 1432, 1486
NmeAIII GCCGAG 2 cut(s) 259, 456
NmuCI GTSAC 4 cut(s) 291, 661, 737, 1001
NruI TCGCGA 1 cut(s) 947
NsiI ATGCAT 3 cut(s) 705, 811, 815
NspI RCATGY 1 cut(s) 813
NspV TTCGAA 1 cut(s) 758
PaeI GCATGC 1 cut(s) 813
PaqCI CACCTGC 1 cut(s) 1354
PceI AGGCCT 1 cut(s) 648
PciSI GCTCTTC 1 cut(s) 1164
PctI GAATGC 1 cut(s) 961
PfeI GAWTC 7 cut(s) 391, 469, 923, 1025, 1158, 1224, 1410
PfoI TCCNGGA 1 cut(s) 706
PkrI GCNGC 2 cut(s) 253, 850
PleI GAGTC 1 cut(s) 298
PpsI GAGTC 1 cut(s) 298
Psp124BI GAGCTC 1 cut(s) 240
Psp6I CCWGG 4 cut(s) 627, 706, 1257, 1374
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 4 cut(s) 627, 706, 1257, 1374
PspPI GGNCC 5 cut(s) 127, 231, 983, 1181, 1355
PsuI RGATCY 1 cut(s) 674
RruI TCGCGA 1 cut(s) 947
RsaI GTAC 3 cut(s) 151, 895, 1097
RsaNI GTAC 3 cut(s) 150, 894, 1096
RseI CAYNNNNRTG 2 cut(s) 1236, 1427
SacI GAGCTC 1 cut(s) 240
SapI GCTCTTC 1 cut(s) 1164
SaqAI TTAA 6 cut(s) 165, 300, 458, 504, 903, 974
SatI GCNGC 2 cut(s) 252, 849
Sau3AI GATC 6 cut(s) 549, 674, 681, 774, 1360, 1440
Sau96I GGNCC 5 cut(s) 127, 231, 983, 1181, 1355
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 6 cut(s) 431, 432, 629, 708, 1259, 1376
SduI GDGCHC 2 cut(s) 240, 573
SfaNI GCATC 8 cut(s) 316, 712, 796, 817, 925, 1051, 1297, 1431
SfuI TTCGAA 1 cut(s) 758
SgrAI CRCCGGYG 1 cut(s) 743
SinI GGWCC 4 cut(s) 127, 983, 1181, 1355
SmaI CCCGGG 1 cut(s) 432
SmiMI CAYNNNNRTG 2 cut(s) 1236, 1427
SmlI CTYRAG 1 cut(s) 572
SmoI CTYRAG 1 cut(s) 572
SphI GCATGC 1 cut(s) 813
Sse9I AATT 8 cut(s) 282, 306, 534, 753, 971, 1036, 1246, 1395
SseBI AGGCCT 1 cut(s) 648
SsiI CCGC 5 cut(s) 183, 218, 229, 848, 1341
SspI AATATT 1 cut(s) 452
SspMI CTAG 3 cut(s) 120, 488, 836
SstI GAGCTC 1 cut(s) 240
StuI AGGCCT 1 cut(s) 648
StyD4I CCNGG 6 cut(s) 429, 430, 627, 706, 1257, 1374
StyI CCWWGG 1 cut(s) 1482
TaaI ACNGT 8 cut(s) 371, 881, 979, 1001, 1213, 1327, 1391, 1453
TaqI TCGA 1 cut(s) 758
TaqII GACCGA 1 cut(s) 1169
TasI AATT 8 cut(s) 282, 306, 534, 753, 971, 1036, 1246, 1395
TatI WGTACW 3 cut(s) 149, 893, 1095
TauI GCSGC 1 cut(s) 851
TfiI GAWTC 7 cut(s) 391, 469, 923, 1025, 1158, 1224, 1410
Tru1I TTAA 6 cut(s) 165, 300, 458, 504, 903, 974
Tru9I TTAA 6 cut(s) 165, 300, 458, 504, 903, 974
TscAI CASTG 7 cut(s) 300, 670, 736, 886, 1006, 1396, 1456
TseFI GTSAC 4 cut(s) 291, 661, 737, 1001
TseI GCWGC 1 cut(s) 251
Tsp45I GTSAC 4 cut(s) 291, 661, 737, 1001
TspDTI ATGAA 1 cut(s) 596
TspGWI ACGGA 2 cut(s) 916, 1359
TspMI CCCGGG 1 cut(s) 430
TspRI CASTG 7 cut(s) 300, 670, 736, 886, 1006, 1396, 1456
VpaK11BI GGWCC 4 cut(s) 127, 983, 1181, 1355
XagI CCTNNNNNAGG 1 cut(s) 245
XapI RAATTY 1 cut(s) 1036
XceI RCATGY 1 cut(s) 813
XmaI CCCGGG 1 cut(s) 430
XspI CTAG 3 cut(s) 120, 488, 836
Zsp2I ATGCAT 3 cut(s) 705, 811, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.