Rh2DG015800

Belongs to the aldehyde dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007245
Physical Location & Seq
Reverse (-)
915204 .. 920158
4955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG015800.1

Sequence Viewer

Length: 1491 bp
ATGGCTGGTACTGGTTTGTTTACAGAGATTTTGGATGGAGAAGCCTACAAGTACTACTCCGATGGAGAATGGAAGAAATCATCTTCTGGTAAACTTGTTCCCATCATCAACCCAACTACAAGAAAGGTTCACTACAAGGTTCAAGCTTGTACACAAGAAGAAGTCAACAAGGTGATGGAGTCAGCAAAAACTGCACAGAAGTTATGGGCAAAGACTCCACTTTGGAAGAGAGCAGAGCTACTTCACAAGGCTGCTTCTATCCTCAAGGAACAAAAAGCTCCAATTGCGGAGTGTTTGGTTAAAGAGATTGCTAAGCCTGCTAAAGATGCTGTTACGGAGGTTGTCAGGTCTGGAGATTTGATCTCTTACACTGCTGAAGAAGGGGTCAGGATCCTTGGTGAAGGGAAGTTCTTGTTGTCTGATAGTTTTCCTGGAAATGAAAGAACCAAGTACTGCCTCACTTCCAAGATTCCACTTGGTGTGGTTCTAGCCATTCCACCATTTAACTATCCTGTCAACCTTGCCGTCTCAAAAATTGCTCCGGCATTGATTGCTGGAAACTCTCTTGTGCTCAAACCCCCAACTCAGGGTGCTGTTTCTGCCCTTCATATGGTACATTGTTTTCACTTGGCTGGTTTCCCCAAAGGCCTTATTAGCTGTGTCACTGGGAAAGGATCTGAGATAGGTGACTTCCTTACCATGCACCCCGGAGTGAATTGTATTAGCTTCACTGGTGGTGACACCGGTGTTGCAATTTCCAAAAAGGCAGGCATGATCCCTCTTCAGATGGAGTTGGGAGGAAAAGATGCCTGCATTGTGCTTGAAGATGCTGATCTTGATTTGGTGGCCGTAAACATAATAAAAGGAGGCTTTTCCTACAGTGGTCAGAGATGTACTGCTGTCAAGGTTGTGTTGGTGATGGAATCTGTTGCTGATGCCCTGGTTGAGAAAGTGAAGGCCAAGGTGGCAAAGTTAACCGTCGGGCCACCCGAGGAGAACTGTGACATCACTCCAGTTGTGACGGAATCATCTGCAAACTATATCGAAGGGTTGGTTATGGATGCAAAACAGAAAGAAGCAACATTTTGCCAGGAGTACAGAAGAGAGGGGAACCTTATCTGGCCCTTGTTGTTAGATAATGTCAGACCTGATATGCGAATTGCTTGGGAAGAGCCATTCGGTCCAGTTTTGCCAGTTATTAGGATTAGTACTGTTGAAGAAGGGATCCACCATTGCAATGCTAGCAATTTCGGACTACAGGGATGTGTGTTCACCAAAGACATCAACAAAGCAATGTTGATTAGTGATGCAATGGAGACAGGAACAGTTCAGATAAACTCGGCGCCTGCTCGTGGTCCAGATCACTTTCCATTTCAGGGCATAAAAGATAGTGGAATTGGGTCACAGGGAATCACCAACAGCATCAATATGATGACAAAGATCAAGACCACTGTAATCAACTTACCAACCCCTTCTTACACCATGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko01100 ko01120 ko01200 map00010 map00030 map01100 map01120 map01200
Pfam Domains
Protein Families
Aldedh

Protein Analysis

496

Amino Acids

53.44

Weight (kDa)

7.08

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldedh PF00171 24 - 483 4e-145 Aldehyde dehydrogenase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1342
AciI CCGC 1 cut(s) 287
AclWI GGATC 6 cut(s) 385, 398, 682, 769, 1219, 1232
AcoI YGGCCR 1 cut(s) 846
AcuI CTGAAG 2 cut(s) 396, 767
AcyI GRCGYC 1 cut(s) 1343
AdeI CACNNNGTG 1 cut(s) 479
AfaI GTAC 8 cut(s) 10, 53, 151, 452, 615, 895, 1097, 1210
AfiI CCNNNNNNNGG 5 cut(s) 586, 587, 610, 1352, 1376
AgeI ACCGGT 1 cut(s) 743
AgsI TTSAA 3 cut(s) 143, 824, 1217
AjnI CCWGG 3 cut(s) 430, 939, 1089
AluBI AGCT 5 cut(s) 146, 238, 278, 657, 726
AluI AGCT 5 cut(s) 146, 238, 278, 657, 726
Alw21I GWGCWC 1 cut(s) 573
Alw26I GTCTC 2 cut(s) 532, 1310
AlwI GGATC 6 cut(s) 385, 398, 682, 769, 1219, 1232
Ama87I CYCGRG 1 cut(s) 989
AoxI GGCC 5 cut(s) 646, 846, 957, 983, 1121
ApeKI GCWGC 1 cut(s) 251
AsiGI ACCGGT 1 cut(s) 743
AspLEI GCGC 1 cut(s) 1345
AspS9I GGNCC 4 cut(s) 983, 1122, 1181, 1355
AsuC2I CCSGG 1 cut(s) 708
AsuHPI GGTGA 7 cut(s) 184, 410, 698, 749, 928, 1264, 1405
AsuNHI GCTAGC 1 cut(s) 1241
AvaI CYCGRG 1 cut(s) 989
AvaII GGWCC 2 cut(s) 1181, 1355
BamHI GGATCC 2 cut(s) 390, 1224
BanI GGYRCC 1 cut(s) 1342
BauI CACGAG 1 cut(s) 1350
Bbv12I GWGCWC 1 cut(s) 573
BbvI GCAGC 1 cut(s) 238
BccI CCATC 6 cut(s) 29, 56, 110, 169, 781, 913
BceAI ACGGC 2 cut(s) 509, 833
BciT130I CCWGG 3 cut(s) 432, 941, 1091
BcnI CCSGG 1 cut(s) 708
BcoDI GTCTC 2 cut(s) 532, 1310
BfaI CTAG 2 cut(s) 488, 1242
BfmI CTRYAG 2 cut(s) 877, 1256
BfoI RGCGCY 1 cut(s) 1346
BglI GCCNNNNNGGC 1 cut(s) 965
BisI GCNGC 1 cut(s) 252
BlpI GCTNAGC 1 cut(s) 312
BlsI GCNGC 1 cut(s) 253
BmcAI AGTACT 3 cut(s) 53, 452, 1210
Bme1390I CCNGG 4 cut(s) 432, 708, 941, 1091
Bme18I GGWCC 2 cut(s) 1181, 1355
BmeT110I CYCGRG 1 cut(s) 989
BmgT120I GGNCC 4 cut(s) 983, 1122, 1181, 1355
BmiI GGNNCC 4 cut(s) 392, 1112, 1226, 1344
BmrFI CCNGG 4 cut(s) 432, 708, 941, 1091
BmrI ACTGGG 1 cut(s) 675
BmsI GCATC 7 cut(s) 316, 796, 817, 925, 1051, 1297, 1431
BmtI GCTAGC 1 cut(s) 1245
BmuI ACTGGG 1 cut(s) 675
BpmI CTGGAG 2 cut(s) 372, 996
Bpu1102I GCTNAGC 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 248
BpuMI CCSGG 1 cut(s) 708
BsaBI GATNNNNATC 1 cut(s) 831
BsaHI GRCGYC 1 cut(s) 1343
BsaJI CCNNGG 6 cut(s) 394, 706, 939, 960, 990, 1482
BsaWI WCCGGW 1 cut(s) 743
Bsc4I CCNNNNNNNGG 5 cut(s) 586, 587, 610, 1352, 1376
Bse118I RCCGGY 1 cut(s) 743
Bse1I ACTGG 6 cut(s) 16, 670, 736, 1013, 1184, 1193
Bse3DI GCAATG 4 cut(s) 1231, 1243, 1299, 1317
Bse8I GATNNNNATC 1 cut(s) 831
BseBI CCWGG 3 cut(s) 432, 941, 1091
BseDI CCNNGG 6 cut(s) 394, 706, 939, 960, 990, 1482
BseGI GGATG 3 cut(s) 40, 1066, 1268
BseJI GATNNNNATC 1 cut(s) 831
BseLI CCNNNNNNNGG 5 cut(s) 586, 587, 610, 1352, 1376
BseMI GCAATG 4 cut(s) 1231, 1243, 1299, 1317
BseMII CTCAG 2 cut(s) 599, 669
BseNI ACTGG 6 cut(s) 16, 670, 736, 1013, 1184, 1193
BseRI GAGGAG 1 cut(s) 1007
BseXI GCAGC 1 cut(s) 238
BsgI GTGCAG 1 cut(s) 177
BshFI GGCC 5 cut(s) 648, 848, 959, 985, 1123
BshNI GGYRCC 1 cut(s) 1342
BshTI ACCGGT 1 cut(s) 743
BsiHKAI GWGCWC 1 cut(s) 573
BsiHKCI CYCGRG 1 cut(s) 989
BsiSI CCGG 3 cut(s) 542, 708, 744
BslI CCNNNNNNNGG 5 cut(s) 586, 587, 610, 1352, 1376
BsmAI GTCTC 2 cut(s) 532, 1310
BsmBI CGTCTC 1 cut(s) 532
BsnI GGCC 5 cut(s) 648, 848, 959, 985, 1123
BsoBI CYCGRG 1 cut(s) 989
Bsp1286I GDGCHC 1 cut(s) 573
Bsp1407I TGTACA 1 cut(s) 149
Bsp143I GATC 8 cut(s) 360, 390, 674, 774, 832, 1224, 1360, 1440
Bsp1720I GCTNAGC 1 cut(s) 312
Bsp19I CCATGG 1 cut(s) 1482
BspACI CCGC 1 cut(s) 287
BspANI GGCC 5 cut(s) 648, 848, 959, 985, 1123
BspCNI CTCAG 2 cut(s) 598, 670
BspLI GGNNCC 4 cut(s) 392, 1112, 1226, 1344
BspOI GCTAGC 1 cut(s) 1245
BspPI GGATC 6 cut(s) 385, 398, 682, 769, 1219, 1232
BspQI GCTCTTC 1 cut(s) 1164
BspT107I GGYRCC 1 cut(s) 1342
BsrDI GCAATG 4 cut(s) 1231, 1243, 1299, 1317
BsrFI RCCGGY 1 cut(s) 743
BsrGI TGTACA 1 cut(s) 149
BsrI ACTGG 6 cut(s) 16, 670, 736, 1013, 1184, 1193
BssAI RCCGGY 1 cut(s) 743
BssECI CCNNGG 6 cut(s) 394, 706, 939, 960, 990, 1482
BssMI GATC 8 cut(s) 360, 390, 674, 774, 832, 1224, 1360, 1440
BssNI GRCGYC 1 cut(s) 1343
BssSI CACGAG 1 cut(s) 1350
BssT1I CCWWGG 3 cut(s) 394, 960, 1482
Bst2BI CACGAG 1 cut(s) 1350
Bst2UI CCWGG 3 cut(s) 432, 941, 1091
Bst4CI ACNGT 6 cut(s) 881, 979, 1001, 1213, 1327, 1453
Bst6I CTCTTC 4 cut(s) 221, 786, 1096, 1164
BstACI GRCGYC 1 cut(s) 1343
BstAPI GCANNNNNTGC 2 cut(s) 191, 551
BstAUI TGTACA 1 cut(s) 149
BstC8I GCNNGC 5 cut(s) 318, 769, 811, 1243, 1347
BstDEI CTNAG 3 cut(s) 312, 585, 678
BstDSI CCRYGG 1 cut(s) 1482
BstF5I GGATG 3 cut(s) 40, 1066, 1268
BstH2I RGCGCY 1 cut(s) 1346
BstHHI GCGC 1 cut(s) 1345
BstKTI GATC 8 cut(s) 363, 393, 677, 777, 835, 1227, 1363, 1443
BstMAI GTCTC 2 cut(s) 532, 1310
BstMBI GATC 8 cut(s) 360, 390, 674, 774, 832, 1224, 1360, 1440
BstMWI GCNNNNNNNGC 9 cut(s) 191, 284, 317, 326, 551, 599, 654, 965, 1242
BstNI CCWGG 3 cut(s) 432, 941, 1091
BstSCI CCNGG 4 cut(s) 430, 706, 939, 1089
BstSFI CTRYAG 2 cut(s) 877, 1256
BstV1I GCAGC 1 cut(s) 238
BstX2I RGATCY 3 cut(s) 390, 674, 1224
BstYI RGATCY 3 cut(s) 390, 674, 1224
BsuRI GGCC 5 cut(s) 648, 848, 959, 985, 1123
BtgI CCRYGG 1 cut(s) 1482
BtsCI GGATG 3 cut(s) 40, 1066, 1268
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 5 cut(s) 369, 663, 729, 886, 1449
Cac8I GCNNGC 5 cut(s) 318, 769, 811, 1243, 1347
CfoI GCGC 1 cut(s) 1345
Cfr10I RCCGGY 1 cut(s) 743
Cfr13I GGNCC 4 cut(s) 983, 1122, 1181, 1355
Csp6I GTAC 8 cut(s) 9, 52, 150, 451, 614, 894, 1096, 1209
CspAI ACCGGT 1 cut(s) 743
CviAII CATG 3 cut(s) 700, 772, 1483
CviQI GTAC 8 cut(s) 9, 52, 150, 451, 614, 894, 1096, 1209
DdeI CTNAG 3 cut(s) 312, 585, 678
DinI GGCGCC 1 cut(s) 1344
DpnI GATC 8 cut(s) 362, 392, 676, 776, 834, 1226, 1362, 1442
DpnII GATC 8 cut(s) 360, 390, 674, 774, 832, 1224, 1360, 1440
DraIII CACNNNGTG 1 cut(s) 479
EaeI YGGCCR 1 cut(s) 846
Eam1104I CTCTTC 4 cut(s) 221, 786, 1096, 1164
EarI CTCTTC 4 cut(s) 221, 786, 1096, 1164
Eco130I CCWWGG 3 cut(s) 394, 960, 1482
Eco147I AGGCCT 1 cut(s) 648
Eco47I GGWCC 2 cut(s) 1181, 1355
Eco57I CTGAAG 2 cut(s) 396, 767
Eco88I CYCGRG 1 cut(s) 989
EcoRII CCWGG 3 cut(s) 430, 939, 1089
EcoT14I CCWWGG 3 cut(s) 394, 960, 1482
EgeI GGCGCC 1 cut(s) 1344
EheI GGCGCC 1 cut(s) 1344
ErhI CCWWGG 3 cut(s) 394, 960, 1482
Esp3I CGTCTC 1 cut(s) 532
FaeI CATG 3 cut(s) 703, 775, 1486
FatI CATG 3 cut(s) 699, 771, 1482
FauNDI CATATG 1 cut(s) 609
Fnu4HI GCNGC 1 cut(s) 252
FokI GGATG 3 cut(s) 47, 1073, 1275
Fsp4HI GCNGC 1 cut(s) 252
FspBI CTAG 2 cut(s) 488, 1242
GlaI GCGC 1 cut(s) 1344
GluI GCNGC 1 cut(s) 252
GsuI CTGGAG 2 cut(s) 372, 996
HaeII RGCGCY 1 cut(s) 1346
HaeIII GGCC 5 cut(s) 648, 848, 959, 985, 1123
HapII CCGG 3 cut(s) 542, 708, 744
HhaI GCGC 1 cut(s) 1345
Hin1I GRCGYC 1 cut(s) 1343
Hin1II CATG 3 cut(s) 703, 775, 1486
Hin6I GCGC 1 cut(s) 1343
HinP1I GCGC 1 cut(s) 1343
HincII GTYRAC 3 cut(s) 166, 517, 975
HindII GTYRAC 3 cut(s) 166, 517, 975
HindIII AAGCTT 1 cut(s) 144
HinfI GANTC 6 cut(s) 179, 214, 469, 923, 1025, 1410
HpaI GTTAAC 1 cut(s) 975
HpaII CCGG 3 cut(s) 542, 708, 744
HphI GGTGA 7 cut(s) 184, 410, 698, 749, 928, 1264, 1405
Hpy166II GTNNAC 9 cut(s) 21, 92, 130, 152, 166, 517, 853, 975, 1272
Hpy188I TCNGA 8 cut(s) 61, 421, 679, 786, 888, 1145, 1253, 1332
Hpy188III TCNNGA 5 cut(s) 351, 388, 836, 1358, 1444
Hpy8I GTNNAC 9 cut(s) 21, 92, 130, 152, 166, 517, 853, 975, 1272
Hpy99I CGWCG 1 cut(s) 983
HpyAV CCTTC 7 cut(s) 374, 395, 614, 949, 1040, 1214, 1482
HpyCH4III ACNGT 6 cut(s) 881, 979, 1001, 1213, 1327, 1453
HpyCH4V TGCA 8 cut(s) 194, 703, 752, 813, 1034, 1064, 1236, 1310
HpyF10VI GCNNNNNNNGC 9 cut(s) 191, 284, 317, 326, 551, 599, 654, 965, 1242
HpyF3I CTNAG 3 cut(s) 312, 585, 678
Hsp92I GRCGYC 1 cut(s) 1343
Hsp92II CATG 3 cut(s) 703, 775, 1486
HspAI GCGC 1 cut(s) 1343
KasI GGCGCC 1 cut(s) 1342
KspAI GTTAAC 1 cut(s) 975
Kzo9I GATC 8 cut(s) 360, 390, 674, 774, 832, 1224, 1360, 1440
LguI GCTCTTC 1 cut(s) 1164
LmnI GCTCC 2 cut(s) 283, 544
Lsp1109I GCAGC 1 cut(s) 238
LweI GCATC 7 cut(s) 316, 796, 817, 925, 1051, 1297, 1431
MaeI CTAG 2 cut(s) 488, 1242
MaeIII GTNAC 7 cut(s) 331, 661, 686, 737, 1001, 1018, 1401
MalI GATC 8 cut(s) 362, 392, 676, 776, 834, 1226, 1362, 1442
MboI GATC 8 cut(s) 360, 390, 674, 774, 832, 1224, 1360, 1440
MfeI CAATTG 1 cut(s) 282
MflI RGATCY 3 cut(s) 390, 674, 1224
MhlI GDGCHC 1 cut(s) 573
MluCI AATT 7 cut(s) 282, 534, 715, 753, 1158, 1246, 1395
Mly113I GGCGCC 1 cut(s) 1343
MlyI GAGTC 2 cut(s) 188, 208
MnlI CCTC 8 cut(s) 272, 331, 467, 789, 791, 860, 985, 1099
MseI TTAA 3 cut(s) 300, 504, 974
MslI CAYNNNNRTG 2 cut(s) 1236, 1427
MspI CCGG 3 cut(s) 542, 708, 744
MspR9I CCNGG 4 cut(s) 432, 708, 941, 1091
MunI CAATTG 1 cut(s) 282
MvaI CCWGG 3 cut(s) 432, 941, 1091
MwoI GCNNNNNNNGC 9 cut(s) 191, 284, 317, 326, 551, 599, 654, 965, 1242
NarI GGCGCC 1 cut(s) 1343
NciI CCSGG 1 cut(s) 708
NcoI CCATGG 1 cut(s) 1482
NdeI CATATG 1 cut(s) 609
NdeII GATC 8 cut(s) 360, 390, 674, 774, 832, 1224, 1360, 1440
NheI GCTAGC 1 cut(s) 1241
NlaIII CATG 3 cut(s) 703, 775, 1486
NlaIV GGNNCC 4 cut(s) 392, 1112, 1226, 1344
NmeAIII GCCGAG 1 cut(s) 1319
NmuCI GTSAC 6 cut(s) 661, 686, 737, 1001, 1018, 1401
PceI AGGCCT 1 cut(s) 648
PciSI GCTCTTC 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 1 cut(s) 987
PfeI GAWTC 4 cut(s) 469, 923, 1025, 1410
PfoI TCCNGGA 1 cut(s) 430
PinAI ACCGGT 1 cut(s) 743
PkrI GCNGC 1 cut(s) 253
PleI GAGTC 2 cut(s) 187, 208
PluTI GGCGCC 1 cut(s) 1346
PpsI GAGTC 2 cut(s) 187, 208
Psp6I CCWGG 3 cut(s) 430, 939, 1089
PspGI CCWGG 3 cut(s) 430, 939, 1089
PspN4I GGNNCC 4 cut(s) 392, 1112, 1226, 1344
PspPI GGNCC 4 cut(s) 983, 1122, 1181, 1355
PsuI RGATCY 3 cut(s) 390, 674, 1224
RsaI GTAC 8 cut(s) 10, 53, 151, 452, 615, 895, 1097, 1210
RsaNI GTAC 8 cut(s) 9, 52, 150, 451, 614, 894, 1096, 1209
RseI CAYNNNNRTG 2 cut(s) 1236, 1427
SapI GCTCTTC 1 cut(s) 1164
SaqAI TTAA 3 cut(s) 300, 504, 974
SatI GCNGC 1 cut(s) 252
Sau3AI GATC 8 cut(s) 360, 390, 674, 774, 832, 1224, 1360, 1440
Sau96I GGNCC 4 cut(s) 983, 1122, 1181, 1355
ScaI AGTACT 3 cut(s) 53, 452, 1210
SchI GAGTC 2 cut(s) 188, 208
ScrFI CCNGG 4 cut(s) 432, 708, 941, 1091
SduI GDGCHC 1 cut(s) 573
SfaNI GCATC 7 cut(s) 316, 796, 817, 925, 1051, 1297, 1431
SfcI CTRYAG 2 cut(s) 877, 1256
SfoI GGCGCC 1 cut(s) 1344
SgrAI CRCCGGYG 1 cut(s) 743
SinI GGWCC 2 cut(s) 1181, 1355
SmiMI CAYNNNNRTG 2 cut(s) 1236, 1427
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 7 cut(s) 282, 534, 715, 753, 1158, 1246, 1395
SseBI AGGCCT 1 cut(s) 648
SsiI CCGC 1 cut(s) 287
SspDI GGCGCC 1 cut(s) 1342
SspMI CTAG 2 cut(s) 488, 1242
StuI AGGCCT 1 cut(s) 648
StyD4I CCNGG 4 cut(s) 430, 706, 939, 1089
StyI CCWWGG 3 cut(s) 394, 960, 1482
TaaI ACNGT 6 cut(s) 881, 979, 1001, 1213, 1327, 1453
TaqI TCGA 1 cut(s) 1044
TaqII GACCGA 1 cut(s) 1169
TasI AATT 7 cut(s) 282, 534, 715, 753, 1158, 1246, 1395
TatI WGTACW 6 cut(s) 51, 149, 450, 893, 1095, 1208
TfiI GAWTC 4 cut(s) 469, 923, 1025, 1410
Tru1I TTAA 3 cut(s) 300, 504, 974
Tru9I TTAA 3 cut(s) 300, 504, 974
TscAI CASTG 5 cut(s) 376, 670, 736, 886, 1456
TseFI GTSAC 6 cut(s) 661, 686, 737, 1001, 1018, 1401
TseI GCWGC 1 cut(s) 251
Tsp45I GTSAC 6 cut(s) 661, 686, 737, 1001, 1018, 1401
TspDTI ATGAA 2 cut(s) 453, 596
TspGWI ACGGA 2 cut(s) 350, 1037
TspRI CASTG 5 cut(s) 376, 670, 736, 886, 1456
VpaK11BI GGWCC 2 cut(s) 1181, 1355
XspI CTAG 2 cut(s) 488, 1242
ZrmI AGTACT 3 cut(s) 53, 452, 1210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.