MD02G1016300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014626
Physical Location & Seq
Reverse (-)
1232013 .. 1234010
1998 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1016300.v1.1.491

Sequence Viewer

Length: 495 bp
ATGACTACTTCATCTTCAGCAGTATCTCTTCTTCTTCTTTTTTCCCTCCTCCTTTCTCAGGCTCAAGGGATTCGTTTGGAGAAAGGATTCATCTCAGTTAGACAGCAGAACAATAATATCCACAAAGAGGAAAATTCTGTGGTGAAAAGAAGTAATATTGCAGCAGCTGGTCTTGCAGGAGAGCCTAGTAATATTGCATCATCAGGAAGCACTAGAAAACTAATCTCTGCCACATCCCCCTCTAGTACTACCACTACCACCACTTCAAAGAATGAGAAGAATGGAAGGGAAAAGAGAGGTGATCCCGAACCGAAAGGCGAAAACATTAAAAGTACTACACAACATGGTGGAAAAAATGGGAATGTCAGAGCAAAATCACCAGTTATTTCTAAGCAGCGGGAGGCTGCTGCAGCTCACCAGCACTATCCGGACCTTGCAGACATGACGGAAATGGACTATTCTCCGGCAAGGAGAAAACCTCCGATACACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

17.62

Weight (kDa)

10.05

Isoelectric Point (pI)

59.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GLV1-2 PF21529 133 - 164 4.1e-08 Gloven 1/2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014626)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01840
malus_domestica MD02G1016300.v1.1 MD15G1160900.v1.1
prunus_persica Prupe.7G256100_v2.0.a1
pyrus_communis pycom02g01450 pycom15g14480
rosa_chinensis RchiOBHm_Chr2g0086811
rosa_laevigata RLG00000015798
rosa_multiflora Rmu_sc0025147.1_g000001 Rmu_sc0027662.1_g000001
rosa_roxburghii Rroxscaffold_2G00154140
rosa_rugosa Rorug01G0469700.1
rosa_samantha Rh2AG022400 Rh2BG022700 Rh2CG023300 Rh2DG022900
rosa_wichuraiana Rw2G001780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 427
AciI CCGC 1 cut(s) 397
AclWI GGATC 1 cut(s) 296
AcsI RAATTY 1 cut(s) 133
AfaI GTAC 2 cut(s) 247, 334
AfiI CCNNNNNNNGG 2 cut(s) 58, 127
AgsI TTSAA 1 cut(s) 267
AluBI AGCT 2 cut(s) 167, 413
AluI AGCT 2 cut(s) 167, 413
AlwI GGATC 1 cut(s) 296
AlwNI CAGNNNCTG 1 cut(s) 167
Aor13HI TCCGGA 1 cut(s) 427
ApeKI GCWGC 6 cut(s) 161, 164, 394, 404, 407, 410
ApoI RAATTY 1 cut(s) 133
Asp700I GAANNNNTTC 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 430
AsuHPI GGTGA 4 cut(s) 154, 311, 369, 407
AvaII GGWCC 1 cut(s) 430
BarI GAAGNNNNNNTAC 2 cut(s) 15, 47
BbvI GCAGC 6 cut(s) 173, 176, 391, 394, 406, 422
BfaI CTAG 3 cut(s) 186, 213, 243
BfmI CTRYAG 1 cut(s) 408
BisI GCNGC 6 cut(s) 162, 165, 395, 405, 408, 411
BlsI GCNGC 6 cut(s) 163, 166, 396, 406, 409, 412
BmcAI AGTACT 2 cut(s) 247, 334
Bme18I GGWCC 1 cut(s) 430
BmgT120I GGNCC 1 cut(s) 430
BmsI GCATC 1 cut(s) 206
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpuEI CTTGAG 1 cut(s) 48
BsaWI WCCGGW 1 cut(s) 427
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 127
Bse1I ACTGG 1 cut(s) 380
BseAI TCCGGA 1 cut(s) 427
BseGI GGATG 1 cut(s) 233
BseLI CCNNNNNNNGG 2 cut(s) 58, 127
BseMII CTCAG 2 cut(s) 71, 108
BseNI ACTGG 1 cut(s) 380
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 6 cut(s) 173, 176, 391, 394, 406, 422
BsiSI CCGG 2 cut(s) 428, 464
BslI CCNNNNNNNGG 2 cut(s) 58, 127
Bsp13I TCCGGA 1 cut(s) 427
Bsp143I GATC 1 cut(s) 301
BspACI CCGC 1 cut(s) 397
BspCNI CTCAG 2 cut(s) 70, 107
BspEI TCCGGA 1 cut(s) 427
BspMAI CTGCAG 1 cut(s) 412
BspPI GGATC 1 cut(s) 296
BsrI ACTGG 1 cut(s) 380
BssMI GATC 1 cut(s) 301
Bst6I CTCTTC 1 cut(s) 33
BstDEI CTNAG 3 cut(s) 57, 94, 390
BstENI CCTNNNNNAGG 1 cut(s) 56
BstF5I GGATG 1 cut(s) 233
BstKTI GATC 1 cut(s) 304
BstMBI GATC 1 cut(s) 301
BstMWI GCNNNNNNNGC 2 cut(s) 173, 410
BstSFI CTRYAG 1 cut(s) 408
BstV1I GCAGC 6 cut(s) 173, 176, 391, 394, 406, 422
BtsCI GGATG 1 cut(s) 233
CaiI CAGNNNCTG 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 430
Csp6I GTAC 2 cut(s) 246, 333
CviAII CATG 2 cut(s) 344, 442
CviJI RGCY 5 cut(s) 62, 167, 184, 404, 413
CviKI_1 RGCY 5 cut(s) 62, 167, 184, 404, 413
CviQI GTAC 2 cut(s) 246, 333
DdeI CTNAG 3 cut(s) 57, 94, 390
DpnI GATC 1 cut(s) 303
DpnII GATC 1 cut(s) 301
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 430
EcoNI CCTNNNNNAGG 1 cut(s) 56
FaeI CATG 2 cut(s) 347, 445
FaiI YATR 2 cut(s) 345, 443
FatI CATG 2 cut(s) 343, 441
FauI CCCGC 1 cut(s) 390
Fnu4HI GCNGC 6 cut(s) 162, 165, 395, 405, 408, 411
FokI GGATG 1 cut(s) 220
Fsp4HI GCNGC 6 cut(s) 162, 165, 395, 405, 408, 411
FspBI CTAG 3 cut(s) 186, 213, 243
GluI GCNGC 6 cut(s) 162, 165, 395, 405, 408, 411
HapII CCGG 2 cut(s) 428, 464
Hin1II CATG 2 cut(s) 347, 445
HinfI GANTC 2 cut(s) 70, 87
HpaII CCGG 2 cut(s) 428, 464
HphI GGTGA 4 cut(s) 154, 311, 369, 407
Hpy188I TCNGA 2 cut(s) 368, 483
Hpy188III TCNNGA 3 cut(s) 204, 305, 428
HpyAV CCTTC 1 cut(s) 279
HpyCH4V TGCA 5 cut(s) 161, 176, 197, 410, 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 173, 410
HpyF3I CTNAG 3 cut(s) 57, 94, 390
Hsp92II CATG 2 cut(s) 347, 445
Kpn2I TCCGGA 1 cut(s) 427
Kzo9I GATC 1 cut(s) 301
LpnPI CCDG 8 cut(s) 44, 153, 162, 189, 393, 431, 441, 477
Lsp1109I GCAGC 6 cut(s) 173, 176, 391, 394, 406, 422
LweI GCATC 1 cut(s) 206
MaeI CTAG 3 cut(s) 186, 213, 243
MalI GATC 1 cut(s) 303
MboI GATC 1 cut(s) 301
MboII GAAGA 5 cut(s) 6, 20, 23, 26, 289
MluCI AATT 1 cut(s) 133
MnlI CCTC 7 cut(s) 56, 59, 121, 250, 290, 394, 489
MroI TCCGGA 1 cut(s) 427
MroXI GAANNNNTTC 1 cut(s) 86
MseI TTAA 1 cut(s) 327
MspA1I CMGCKG 2 cut(s) 167, 397
MspI CCGG 2 cut(s) 428, 464
MwoI GCNNNNNNNGC 2 cut(s) 173, 410
NdeII GATC 1 cut(s) 301
NlaIII CATG 2 cut(s) 347, 445
PdmI GAANNNNTTC 1 cut(s) 86
PfeI GAWTC 2 cut(s) 70, 87
PkrI GCNGC 6 cut(s) 163, 166, 396, 406, 409, 412
PspPI GGNCC 1 cut(s) 430
PstI CTGCAG 1 cut(s) 412
PstNI CAGNNNCTG 1 cut(s) 167
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 2 cut(s) 247, 334
RsaNI GTAC 2 cut(s) 246, 333
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 6 cut(s) 162, 165, 395, 405, 408, 411
Sau3AI GATC 1 cut(s) 301
Sau96I GGNCC 1 cut(s) 430
ScaI AGTACT 2 cut(s) 247, 334
SetI ASST 5 cut(s) 169, 301, 415, 435, 481
SfaNI GCATC 1 cut(s) 206
SfcI CTRYAG 1 cut(s) 408
SinI GGWCC 1 cut(s) 430
SmlI CTYRAG 1 cut(s) 63
SmoI CTYRAG 1 cut(s) 63
Sse9I AATT 1 cut(s) 133
SsiI CCGC 1 cut(s) 397
SspI AATATT 2 cut(s) 157, 193
SspMI CTAG 3 cut(s) 186, 213, 243
TasI AATT 1 cut(s) 133
TatI WGTACW 2 cut(s) 245, 332
TfiI GAWTC 2 cut(s) 70, 87
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TseI GCWGC 6 cut(s) 161, 164, 394, 404, 407, 410
TspDTI ATGAA 1 cut(s) 79
TspGWI ACGGA 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 430
XagI CCTNNNNNAGG 1 cut(s) 56
XapI RAATTY 1 cut(s) 133
XmnI GAANNNNTTC 1 cut(s) 86
XspI CTAG 3 cut(s) 186, 213, 243
ZrmI AGTACT 2 cut(s) 247, 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.