Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0010277

Physical Location & Seq

Genomic Coordinates Forward (+)

1496528 .. 1500542

Gene Structure 4015 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1021200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1461 bp

ATGATGGCAGCGGTTACTAGGATGGAGTCCGCGTTGCTTGGAATTATTCTTACCTCTTTTGTCGCGTTGAGCTTCTTGATGAATGGGAATGCAGCGGGAATCACGAGTACTTTCATCCGATCTGAGTATCCATCGGTTGATATACCTCTGGACCATAAAGTATTTGCGGTGCCAAAGGGTTACAATGCTCCACAACAAGTGCATATCACCCAGGGTGACTATGATGGAAAGGCTGTAATCATCTCATGGGTTACCGCTGACAAACCAGGGGATGGTAAAGTGCAGTATGGTACCTCAGAGAAAAAATACAAGTTTTCTGCTAAGGGGGTCATGACAAACTACACCTTCTACCAGTATAAGTCTGGCTACATCCATCACTGTCTTGTTGATGGCCTAGAGTATGACACAAAGTACTATTACAAAATTGGAAATGGTGATTCATCTCGAGAATTTTGGTTTACAACGCCTCCAAAGATCGATCCAAATTCTCATTACACATTTGGGATCATAGGTGATTTGGGTCAGACATTTAACTCTCTCTCAACTCTTGAGCACTATATGAAGAGTGCAGGACAGGCTGTTTTATTCGTCGGAGATCTTAGCTATGCTGATAGATATCAGTATAATGACGTAGGTTTACGGTGGGACACATGGGCTCGATTCGTCGAGCAAAGTACTGCATATCAGTCATGGATGTGGTCTGCTGGCAATCATGAAATTGATTACATGCCTTATATGGGAGAAGTTACTCCGTTCAAGAACTATCTTCAGCGGTATGCTACTCCGTATTTGGCCTCTAGTAGTAGCAACCCTCTTTGGTATGCCATCAGACGCGCATCAGCTCACATAATCGTGTTGTCCAGTTACTCTCCATTTGTGAAGTACACGCCTCAATGGATGTGGCTAGCAGCAGAATTGCAAAGGGTTGATAGGAAAAAAACACCTTGGCTCATAGTCCTTATGCATGTTCCGATGTACAATAGTAATGAGGCTCATTACATGGAAGGTGAAAGCATGCGAGCAGTGTTTGAGAGTTGGTTCGTTCAATACAAAGTTGATGTGGTGTTTGCTGGCCACGTCCATGCTTATGAGAGATCGTATCGTTTCTCCAATATACAATACAACGTAACAAGTGGTTACCAGTACCCCGTACCAGACAAATCAGCTCCTGTGTACATAACTGTTGGAGATGGAGGGAATCAAGAAGGACTTGCTGGACCGTTTAGAGATCCACAACCAGATTATTCTGCATTCCGAGAAGCAAGCTACGGGCATTCCACGCTTGAGCTGATAAACAGGACACACGCACTCTACCATTGGAACCGCAATGACGATGGGAAGAAAACCGCCATGGATGCATTCGTGTTACACAACCAATACTGGGGTGCGAATCACGGGAGGCGAAAACTGGAGAAGCATAATGTTAGGAAGATAATGATGGACGAAAGTGCTACGTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009505 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0030312 GO:0042578 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0048878 GO:0050801 GO:0055062 GO:0055081 GO:0055083 GO:0065007 GO:0065008 GO:0071944 GO:0072505 GO:0072506 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

487

Amino Acids

55.79

Weight (kDa)

7.32

Isoelectric Point (pI)

38.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pur_ac_phosph_N	PF16656	64 - 155	5.5e-24	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	166 - 363	1.7e-22	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	389 - 447	4.1e-20	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010277)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G34850
fragaria_vesca	FvH4_1g02440 FvH4_1g02440
malus_domestica	MD02G1021200.v1.1 MD15G1164300.v1.1
prunus_persica	Prupe.7G250300_v2.0.a1 Prupe.7G250300_v2.0.a1 Prupe.7G250300_v2.0.a1
pyrus_communis	pycom02g01880 pycom15g14740
rosa_chinensis	RchiOBHm_Chr2g0087401
rosa_laevigata	RLG00000015863
rosa_multiflora	Rmu_sc0006064.1_g000007
rosa_roxburghii	Rroxscaffold_2G00153580
rosa_rugosa	Rorug01G0474900 Rorug01G0475000
rosa_samantha	Rh2AG028400 Rh2BG028300 Rh2CG028700 Rh2DG028500
rosa_wichuraiana	Rw2G002260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	290
AccB1I	GGYRCC	2 cut(s)	169, 290
AccB7I	CCANNNNNTGG	1 cut(s)	272
AccII	CGCG	3 cut(s)	32, 65, 834
AciI	CCGC	8 cut(s)	11, 30, 95, 167, 255, 772, 1324, 1347
AclWI	GGATC	3 cut(s)	473, 512, 1223
AcoI	YGGCCR	1 cut(s)	1072
AcsI	RAATTY	2 cut(s)	449, 484
AcuI	CTGAAG	1 cut(s)	752
AfaI	GTAC	9 cut(s)	109, 292, 413, 676, 884, 977, 1145, 1152, 1175
AfiI	CCNNNNNNNGG	3 cut(s)	272, 737, 1381
AgsI	TTSAA	2 cut(s)	757, 1046
AjiI	CACGTC	1 cut(s)	1078
AjnI	CCWGG	2 cut(s)	210, 265
AluBI	AGCT	6 cut(s)	72, 603, 842, 1166, 1266, 1288
AluI	AGCT	6 cut(s)	72, 603, 842, 1166, 1266, 1288
Alw21I	GWGCWC	1 cut(s)	555
AlwI	GGATC	3 cut(s)	473, 512, 1223
AlwNI	CAGNNNCTG	1 cut(s)	1169
Ama87I	CYCGRG	1 cut(s)	444
AoxI	GGCC	3 cut(s)	391, 792, 1072
ApeKI	GCWGC	3 cut(s)	8, 92, 908
ApoI	RAATTY	2 cut(s)	449, 484
Asp718I	GGTACC	1 cut(s)	290
AspLEI	GCGC	1 cut(s)	836
AspS9I	GGNCC	2 cut(s)	151, 1217
AsuHPI	GGTGA	5 cut(s)	199, 227, 446, 524, 1019
AsuNHI	GCTAGC	1 cut(s)	904
AvaI	CYCGRG	1 cut(s)	444
AvaII	GGWCC	2 cut(s)	151, 1217
BalI	TGGCCA	1 cut(s)	1074
BanI	GGYRCC	2 cut(s)	169, 290
BanII	GRGCYC	1 cut(s)	658
BauI	CACGAG	1 cut(s)	103
Bbv12I	GWGCWC	1 cut(s)	555
BbvI	GCAGC	3 cut(s)	20, 104, 920
BciT130I	CCWGG	2 cut(s)	212, 267
BciVI	GTATCC	1 cut(s)	138
BfaI	CTAG	4 cut(s)	18, 395, 798, 905
BfuI	GTATCC	1 cut(s)	138
BglII	AGATCT	1 cut(s)	595
BisI	GCNGC	3 cut(s)	9, 93, 909
BlsI	GCNGC	3 cut(s)	10, 94, 910
BmcAI	AGTACT	3 cut(s)	109, 413, 676
Bme1390I	CCNGG	2 cut(s)	212, 267
Bme18I	GGWCC	2 cut(s)	151, 1217
BmeT110I	CYCGRG	1 cut(s)	444
BmgBI	CACGTC	1 cut(s)	1078
BmgT120I	GGNCC	2 cut(s)	151, 1217
BmiI	GGNNCC	3 cut(s)	171, 292, 1322
BmrFI	CCNGG	2 cut(s)	212, 267
BmrI	ACTGGG	1 cut(s)	1390
BmsI	GCATC	2 cut(s)	845, 1345
BmtI	GCTAGC	1 cut(s)	908
BmuI	ACTGGG	1 cut(s)	1390
BpmI	CTGGAG	1 cut(s)	1430
Bpu10I	CCTNAGC	1 cut(s)	321
BpuEI	CTTGAG	2 cut(s)	569, 1304
Bsa29I	ATCGAT	1 cut(s)	477
BsaAI	YACGTR	1 cut(s)	1455
BsaBI	GATNNNNATC	1 cut(s)	615
BsaJI	CCNNGG	5 cut(s)	210, 211, 266, 944, 1350
BsaXI	ACNNNNNCTCC	4 cut(s)	17, 47, 451, 481
Bsc4I	CCNNNNNNNGG	3 cut(s)	272, 737, 1381
Bse1I	ACTGG	5 cut(s)	352, 861, 1141, 1385, 1413
Bse3DI	GCAATG	1 cut(s)	1333
Bse8I	GATNNNNATC	1 cut(s)	615
BseBI	CCWGG	2 cut(s)	212, 267
BseCI	ATCGAT	1 cut(s)	477
BseDI	CCNNGG	5 cut(s)	210, 211, 266, 944, 1350
BseGI	GGATG	7 cut(s)	27, 114, 277, 369, 699, 903, 1360
BseJI	GATNNNNATC	1 cut(s)	615
BseLI	CCNNNNNNNGG	3 cut(s)	272, 737, 1381
BseMI	GCAATG	1 cut(s)	1333
BseMII	CTCAG	2 cut(s)	114, 309
BseNI	ACTGG	5 cut(s)	352, 861, 1141, 1385, 1413
BseXI	GCAGC	3 cut(s)	20, 104, 920
BsgI	GTGCAG	2 cut(s)	302, 588
Bsh1236I	CGCG	3 cut(s)	32, 65, 834
BshFI	GGCC	3 cut(s)	393, 794, 1074
BshNI	GGYRCC	2 cut(s)	169, 290
BshVI	ATCGAT	1 cut(s)	477
BsiHKAI	GWGCWC	1 cut(s)	555
BsiHKCI	CYCGRG	1 cut(s)	444
BslFI	GGGAC	1 cut(s)	659
BslI	CCNNNNNNNGG	3 cut(s)	272, 737, 1381
BsmFI	GGGAC	1 cut(s)	659
BsmI	GAATGC	4 cut(s)	94, 1250, 1273, 1358
BsnI	GGCC	3 cut(s)	393, 794, 1074
BsoBI	CYCGRG	1 cut(s)	444
Bsp1286I	GDGCHC	2 cut(s)	555, 658
Bsp1407I	TGTACA	2 cut(s)	975, 1173
Bsp143I	GATC	7 cut(s)	119, 474, 478, 504, 595, 1094, 1228
Bsp19I	CCATGG	1 cut(s)	1350
BspACI	CCGC	8 cut(s)	11, 30, 95, 167, 255, 772, 1324, 1347
BspANI	GGCC	3 cut(s)	393, 794, 1074
BspCNI	CTCAG	2 cut(s)	115, 308
BspDI	ATCGAT	1 cut(s)	477
BspFNI	CGCG	3 cut(s)	32, 65, 834
BspHI	TCATGA	2 cut(s)	330, 712
BspLI	GGNNCC	3 cut(s)	171, 292, 1322
BspOI	GCTAGC	1 cut(s)	908
BspPI	GGATC	3 cut(s)	473, 512, 1223
BspT107I	GGYRCC	2 cut(s)	169, 290
BsrDI	GCAATG	1 cut(s)	1333
BsrGI	TGTACA	2 cut(s)	975, 1173
BsrI	ACTGG	5 cut(s)	352, 861, 1141, 1385, 1413
BssECI	CCNNGG	5 cut(s)	210, 211, 266, 944, 1350
BssMI	GATC	7 cut(s)	119, 474, 478, 504, 595, 1094, 1228
BssSI	CACGAG	1 cut(s)	103
BssT1I	CCWWGG	2 cut(s)	944, 1350
Bst2BI	CACGAG	1 cut(s)	103
Bst2UI	CCWGG	2 cut(s)	212, 267
Bst4CI	ACNGT	4 cut(s)	380, 642, 1183, 1221
Bst6I	CTCTTC	1 cut(s)	557
BstAUI	TGTACA	2 cut(s)	975, 1173
BstBAI	YACGTR	1 cut(s)	1455
BstC8I	GCNNGC	6 cut(s)	706, 906, 1016, 1020, 1072, 1264
BstDEI	CTNAG	4 cut(s)	123, 295, 321, 599
BstDSI	CCRYGG	1 cut(s)	1350
BstEII	GGTNACC	2 cut(s)	250, 1136
BstF5I	GGATG	7 cut(s)	27, 114, 277, 369, 699, 903, 1360
BstFNI	CGCG	3 cut(s)	32, 65, 834
BstHHI	GCGC	1 cut(s)	836
BstKTI	GATC	7 cut(s)	122, 477, 481, 507, 598, 1097, 1231
BstMBI	GATC	7 cut(s)	119, 474, 478, 504, 595, 1094, 1228
BstMWI	GCNNNNNNNGC	3 cut(s)	575, 1279, 1355
BstNI	CCWGG	2 cut(s)	212, 267
BstNSI	RCATGY	3 cut(s)	730, 968, 1018
BstPI	GGTNACC	2 cut(s)	250, 1136
BstSCI	CCNGG	2 cut(s)	210, 265
BstSNI	TACGTA	1 cut(s)	1455
BstUI	CGCG	3 cut(s)	32, 65, 834
BstV1I	GCAGC	3 cut(s)	20, 104, 920
BstX2I	RGATCY	2 cut(s)	595, 1228
BstYI	RGATCY	2 cut(s)	595, 1228
Bsu15I	ATCGAT	1 cut(s)	477
BsuI	GTATCC	1 cut(s)	138
BsuRI	GGCC	3 cut(s)	393, 794, 1074
BsuTUI	ATCGAT	1 cut(s)	477
BtgI	CCRYGG	1 cut(s)	1350
BtrI	CACGTC	1 cut(s)	1078
BtsCI	GGATG	7 cut(s)	27, 114, 277, 369, 699, 903, 1360
BtsI	GCAGTG	1 cut(s)	1029
BtsIMutI	CAGTG	2 cut(s)	376, 1029
Cac8I	GCNNGC	6 cut(s)	706, 906, 1016, 1020, 1072, 1264
CaiI	CAGNNNCTG	1 cut(s)	1169
CciI	TCATGA	2 cut(s)	330, 712
CfoI	GCGC	1 cut(s)	836
Cfr13I	GGNCC	2 cut(s)	151, 1217
ClaI	ATCGAT	1 cut(s)	477
CseI	GACGC	1 cut(s)	840
Csp6I	GTAC	9 cut(s)	108, 291, 412, 675, 883, 976, 1144, 1151, 1174
CspCI	CAANNNNNGTGG	2 cut(s)	881, 916
CviQI	GTAC	9 cut(s)	108, 291, 412, 675, 883, 976, 1144, 1151, 1174
DdeI	CTNAG	4 cut(s)	123, 295, 321, 599
DpnI	GATC	7 cut(s)	121, 476, 480, 506, 597, 1096, 1230
DpnII	GATC	7 cut(s)	119, 474, 478, 504, 595, 1094, 1228
EaeI	YGGCCR	1 cut(s)	1072
Eam1104I	CTCTTC	1 cut(s)	557
EarI	CTCTTC	1 cut(s)	557
Eco105I	TACGTA	1 cut(s)	1455
Eco130I	CCWWGG	2 cut(s)	944, 1350
Eco24I	GRGCYC	1 cut(s)	658
Eco32I	GATATC	1 cut(s)	617
Eco47I	GGWCC	2 cut(s)	151, 1217
Eco57I	CTGAAG	1 cut(s)	752
Eco88I	CYCGRG	1 cut(s)	444
Eco91I	GGTNACC	2 cut(s)	250, 1136
EcoO65I	GGTNACC	2 cut(s)	250, 1136
EcoRII	CCWGG	2 cut(s)	210, 265
EcoRV	GATATC	1 cut(s)	617
EcoT14I	CCWWGG	2 cut(s)	944, 1350
EcoT22I	ATGCAT	2 cut(s)	966, 1360
EcoT38I	GRGCYC	1 cut(s)	658
ErhI	CCWWGG	2 cut(s)	944, 1350
FalI	AAGNNNNNCTT	2 cut(s)	1194, 1226
FaqI	GGGAC	1 cut(s)	659
FauI	CCCGC	1 cut(s)	88
Fnu4HI	GCNGC	3 cut(s)	9, 93, 909
FokI	GGATG	7 cut(s)	34, 101, 284, 356, 706, 910, 1367
FriOI	GRGCYC	1 cut(s)	658
Fsp4HI	GCNGC	3 cut(s)	9, 93, 909
FspBI	CTAG	4 cut(s)	18, 395, 798, 905
GlaI	GCGC	1 cut(s)	835
GluI	GCNGC	3 cut(s)	9, 93, 909
GsuI	CTGGAG	1 cut(s)	1430
HaeIII	GGCC	3 cut(s)	393, 794, 1074
HgaI	GACGC	1 cut(s)	840
HhaI	GCGC	1 cut(s)	836
Hin6I	GCGC	1 cut(s)	834
HinP1I	GCGC	1 cut(s)	834
HinfI	GANTC	6 cut(s)	26, 99, 437, 660, 1198, 1390
HphI	GGTGA	5 cut(s)	199, 227, 446, 524, 1019
Hpy166II	GTNNAC	4 cut(s)	459, 638, 885, 1174
Hpy188I	TCNGA	8 cut(s)	119, 124, 298, 525, 593, 830, 972, 1256
Hpy8I	GTNNAC	4 cut(s)	459, 638, 885, 1174
Hpy99I	CGWCG	2 cut(s)	593, 668
HpyAV	CCTTC	3 cut(s)	355, 998, 1199
HpyCH4III	ACNGT	4 cut(s)	380, 642, 1183, 1221
HpyCH4IV	ACGT	4 cut(s)	630, 1077, 1125, 1454
HpyCH4V	TGCA	9 cut(s)	92, 202, 283, 569, 680, 919, 964, 1250, 1358
HpyF10VI	GCNNNNNNNGC	3 cut(s)	575, 1279, 1355
HpyF3I	CTNAG	4 cut(s)	123, 295, 321, 599
HpySE526I	ACGT	4 cut(s)	630, 1077, 1125, 1454
HspAI	GCGC	1 cut(s)	834
KpnI	GGTACC	1 cut(s)	294
Kzo9I	GATC	7 cut(s)	119, 474, 478, 504, 595, 1094, 1228
LmnI	GCTCC	2 cut(s)	193, 1171
Lsp1109I	GCAGC	3 cut(s)	20, 104, 920
LweI	GCATC	2 cut(s)	845, 1345
MaeI	CTAG	4 cut(s)	18, 395, 798, 905
MaeII	ACGT	4 cut(s)	630, 1077, 1125, 1454
MaeIII	GTNAC	9 cut(s)	13, 179, 215, 250, 745, 863, 1126, 1136, 1365
MalI	GATC	7 cut(s)	121, 476, 480, 506, 597, 1096, 1230
MboI	GATC	7 cut(s)	119, 474, 478, 504, 595, 1094, 1228
MboII	GAAGA	4 cut(s)	574, 758, 1351, 1441
MflI	RGATCY	2 cut(s)	595, 1228
MhlI	GDGCHC	2 cut(s)	555, 658
MlsI	TGGCCA	1 cut(s)	1074
MluCI	AATT	6 cut(s)	42, 423, 449, 484, 717, 914
MluNI	TGGCCA	1 cut(s)	1074
MlyI	GAGTC	1 cut(s)	35
MmeI	TCCRAC	2 cut(s)	571, 1165
Mox20I	TGGCCA	1 cut(s)	1074
Mph1103I	ATGCAT	2 cut(s)	966, 1360
MscI	TGGCCA	1 cut(s)	1074
MseI	TTAA	1 cut(s)	531
MslI	CAYNNNNRTG	4 cut(s)	694, 851, 1080, 1086
Msp20I	TGGCCA	1 cut(s)	1074
MspA1I	CMGCKG	4 cut(s)	11, 95, 257, 772
MspR9I	CCNGG	2 cut(s)	212, 267
Mva1269I	GAATGC	4 cut(s)	94, 1250, 1273, 1358
MvaI	CCWGG	2 cut(s)	212, 267
MvnI	CGCG	3 cut(s)	32, 65, 834
MwoI	GCNNNNNNNGC	3 cut(s)	575, 1279, 1355
NcoI	CCATGG	1 cut(s)	1350
NdeII	GATC	7 cut(s)	119, 474, 478, 504, 595, 1094, 1228
NheI	GCTAGC	1 cut(s)	904
NlaIV	GGNNCC	3 cut(s)	171, 292, 1322
NmuCI	GTSAC	1 cut(s)	215
NsiI	ATGCAT	2 cut(s)	966, 1360
NspI	RCATGY	3 cut(s)	730, 968, 1018
PaeI	GCATGC	1 cut(s)	1018
PaeR7I	CTCGAG	1 cut(s)	444
PagI	TCATGA	2 cut(s)	330, 712
PasI	CCCWGGG	1 cut(s)	211
PctI	GAATGC	4 cut(s)	94, 1250, 1273, 1358
PfeI	GAWTC	5 cut(s)	99, 437, 660, 1198, 1390
PflMI	CCANNNNNTGG	1 cut(s)	272
PkrI	GCNGC	3 cut(s)	10, 94, 910
PleI	GAGTC	1 cut(s)	34
PpsI	GAGTC	1 cut(s)	34
Ppu21I	YACGTR	1 cut(s)	1455
Psp6I	CCWGG	2 cut(s)	210, 265
PspEI	GGTNACC	2 cut(s)	250, 1136
PspGI	CCWGG	2 cut(s)	210, 265
PspN4I	GGNNCC	3 cut(s)	171, 292, 1322
PspPI	GGNCC	2 cut(s)	151, 1217
PstNI	CAGNNNCTG	1 cut(s)	1169
PsuI	RGATCY	2 cut(s)	595, 1228
RsaI	GTAC	9 cut(s)	109, 292, 413, 676, 884, 977, 1145, 1152, 1175
RsaNI	GTAC	9 cut(s)	108, 291, 412, 675, 883, 976, 1144, 1151, 1174
RseI	CAYNNNNRTG	4 cut(s)	694, 851, 1080, 1086
SaqAI	TTAA	1 cut(s)	531
SatI	GCNGC	3 cut(s)	9, 93, 909
Sau3AI	GATC	7 cut(s)	119, 474, 478, 504, 595, 1094, 1228
Sau96I	GGNCC	2 cut(s)	151, 1217
ScaI	AGTACT	3 cut(s)	109, 413, 676
SchI	GAGTC	1 cut(s)	35
ScrFI	CCNGG	2 cut(s)	212, 267
SduI	GDGCHC	2 cut(s)	555, 658
SfaNI	GCATC	2 cut(s)	845, 1345
Sfr274I	CTCGAG	1 cut(s)	444
SinI	GGWCC	2 cut(s)	151, 1217
SlaI	CTCGAG	1 cut(s)	444
SmiMI	CAYNNNNRTG	4 cut(s)	694, 851, 1080, 1086
SmlI	CTYRAG	3 cut(s)	444, 548, 1283
SmoI	CTYRAG	3 cut(s)	444, 548, 1283
SnaBI	TACGTA	1 cut(s)	1455
SphI	GCATGC	1 cut(s)	1018
Sse9I	AATT	6 cut(s)	42, 423, 449, 484, 717, 914
SsiI	CCGC	8 cut(s)	11, 30, 95, 167, 255, 772, 1324, 1347
SspMI	CTAG	4 cut(s)	18, 395, 798, 905
StyD4I	CCNGG	2 cut(s)	210, 265
StyI	CCWWGG	2 cut(s)	944, 1350
TaaI	ACNGT	4 cut(s)	380, 642, 1183, 1221
TaiI	ACGT	4 cut(s)	633, 1080, 1128, 1457
TaqI	TCGA	4 cut(s)	445, 477, 658, 666
TasI	AATT	6 cut(s)	42, 423, 449, 484, 717, 914
TatI	WGTACW	6 cut(s)	107, 411, 674, 882, 975, 1173
TfiI	GAWTC	5 cut(s)	99, 437, 660, 1198, 1390
Tru1I	TTAA	1 cut(s)	531
Tru9I	TTAA	1 cut(s)	531
TscAI	CASTG	2 cut(s)	383, 1029
TseFI	GTSAC	1 cut(s)	215
TseI	GCWGC	3 cut(s)	8, 92, 908
Tsp45I	GTSAC	1 cut(s)	215
TspDTI	ATGAA	5 cut(s)	95, 103, 429, 575, 729
TspGWI	ACGGA	2 cut(s)	741, 774
TspRI	CASTG	2 cut(s)	383, 1029
Van91I	CCANNNNNTGG	1 cut(s)	272
VpaK11BI	GGWCC	2 cut(s)	151, 1217
XapI	RAATTY	2 cut(s)	449, 484
XceI	RCATGY	3 cut(s)	730, 968, 1018
XcmI	CCANNNNNNNNNTGG	1 cut(s)	359
XhoI	CTCGAG	1 cut(s)	444
XspI	CTAG	4 cut(s)	18, 395, 798, 905
ZrmI	AGTACT	3 cut(s)	109, 413, 676
Zsp2I	ATGCAT	2 cut(s)	966, 1360

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.