Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010277

Physical Location & Seq

Genomic Coordinates Forward (+)

12369682 .. 12373858

Gene Structure 4177 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1164300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1458 bp

ATGACGGCAGTGGTTACTAGGACGGAGTCCACGCTGCTTCGAATTATCCTTACCTCCTTCGTCTTGTTGAGCCTTTTGAGGAGTGGGAATGCTGCAGGAATCACGAGTACTTTCATTCGATCTGAGCATCCATCGGTTGACATCCCTCTGAACAATAAAGTATTTGCGGTGCCGAAGGGTTACAATGCTCCACAACAAGTGCATATCACCCAAGGTGACTATGATGGAAAGGCTGTAATCATCTCATGGGTTACGGCCAACGAACCAGGGGATAGTAAAGTGCAGTATGGTACATCAGAGAAAAAATACGAGTTTTCTGCTAAGGGTGTCGTGACAAACTACACCTTCTACCAGTATAAGTCTGGCTACATCCATCACTGTCTTGTCGAAGGCCTAGAGTATGACACAAAGTACTATTACAAAATTGGAAATGGCGATTCATCTCGAGAATTTTGGTTTACAACACCTCCAAAGATCGATCCAAATTCTCATTACACATTTGGGATCATAGGTGATTTGGGTCAGACATTTAACTCTCTGTCAACTCTTGAGCACTACATGAAGAGTTCAGGACAGGCTGTTTTATTTGTTGGAGATCTTTCCTATGCTGATAGATATCAGTATAATGAAGTGGGTTTACGGTGGGACACATGGGCTCGATTCATCGAGCAAAGTACTGCGTATCAGCCATGGATGTGGTCTGCTGGCAATCATGAAATTGATTACATGCCTTATATGGGAGAAGTTACTCCGTTCAAGAACTATCTTCATCGGTATGCTACTCCTCATTTGGCCTCTAATAGTAGCAATCCTCTTTGGTATGCCATCAGACGTGCATCAGCTCATATAATCGTGTTGTCCAGTTACTCTCCATTTGTGAAGTACACGCCTCAATGGAGTTGGCTAGCAGAAGAATTGAAAAGGGTTGACAGGGAAAAGACACCTTGGCTCATAGTCCTTATGCATGTTCCATTATACAATAGTAACGACGTTCATTACATGGAAGGTGAAAGCATGCGAGCAGTGTTTGAGAGATGGTTCGTTCAATACAAAGTTGATGTGGTGTTTGCTGGCCATGTCCATGCTTATGAGAGATCGTATCGAATCTCCAATATACGATACAACGTAACAAGCGGTTACCAGTACCCGATACCAGACAAATCGGCTCCTGTATACATAACTGTTGGAGATGGAGGGAACCAAGAAGGCCTTGCTGGAAGGTTTAGGAATCCACAACCAGATTATTCTGCATTCCGAGAAGCCAGCTACGGGCATTCCACGCTTGAGCTTATAAACAGGACACACGCACTCTATCATTGGAACCGCAATGATGATGGGAAGAAAACCGCAATGGACGCATTTATATTAGACAACCAGTACTGGAGTGCGAATCACAGGAGGCGAAAACTGAAGAAGCATAATGTTAGGAAGACAATGGACGAAATTGCTATGTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009505 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0030312 GO:0042578 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0048878 GO:0050801 GO:0055062 GO:0055081 GO:0055083 GO:0065007 GO:0065008 GO:0071944 GO:0072505 GO:0072506 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

486

Amino Acids

56.1

Weight (kDa)

8.66

Isoelectric Point (pI)

42.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pur_ac_phosph_N	PF16656	64 - 155	4.2e-24	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	166 - 363	6.2e-24	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	389 - 447	5.5e-20	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010277)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G34850
fragaria_vesca	FvH4_1g02440 FvH4_1g02440
malus_domestica	MD02G1021200.v1.1 MD15G1164300.v1.1
prunus_persica	Prupe.7G250300_v2.0.a1 Prupe.7G250300_v2.0.a1 Prupe.7G250300_v2.0.a1
pyrus_communis	pycom02g01880 pycom15g14740
rosa_chinensis	RchiOBHm_Chr2g0087401
rosa_laevigata	RLG00000015863
rosa_multiflora	Rmu_sc0006064.1_g000007
rosa_roxburghii	Rroxscaffold_2G00153580
rosa_rugosa	Rorug01G0474900 Rorug01G0475000
rosa_samantha	Rh2AG028400 Rh2BG028300 Rh2CG028700 Rh2DG028500
rosa_wichuraiana	Rw2G002260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1292
AccB1I	GGYRCC	1 cut(s)	169
AccI	GTMKAC	1 cut(s)	1173
AciI	CCGC	4 cut(s)	167, 1134, 1324, 1347
AclWI	GGATC	2 cut(s)	473, 512
AcoI	YGGCCR	2 cut(s)	255, 1072
AcsI	RAATTY	2 cut(s)	449, 484
AcuI	CTGAAG	1 cut(s)	1430
AfaI	GTAC	7 cut(s)	109, 292, 413, 676, 884, 1145, 1379
AfiI	CCNNNNNNNGG	2 cut(s)	737, 1269
AgsI	TTSAA	3 cut(s)	757, 919, 1046
AjiI	CACGTC	1 cut(s)	833
AjnI	CCWGG	1 cut(s)	265
AluBI	AGCT	3 cut(s)	842, 1266, 1288
AluI	AGCT	3 cut(s)	842, 1266, 1288
Alw21I	GWGCWC	1 cut(s)	555
AlwI	GGATC	2 cut(s)	473, 512
Ama87I	CYCGRG	1 cut(s)	444
AoxI	GGCC	5 cut(s)	255, 391, 792, 1072, 1207
ApeKI	GCWGC	2 cut(s)	34, 92
ApoI	RAATTY	2 cut(s)	449, 484
AsuHPI	GGTGA	4 cut(s)	199, 227, 524, 1019
AsuII	TTCGAA	1 cut(s)	40
AsuNHI	GCTAGC	1 cut(s)	904
AvaI	CYCGRG	1 cut(s)	444
BalI	TGGCCA	1 cut(s)	1074
BanI	GGYRCC	1 cut(s)	169
BanII	GRGCYC	1 cut(s)	658
BarI	GAAGNNNNNNTAC	2 cut(s)	621, 653
BauI	CACGAG	1 cut(s)	103
BbsI	GAAGAC	1 cut(s)	1436
Bbv12I	GWGCWC	1 cut(s)	555
BbvI	GCAGC	2 cut(s)	21, 79
BccI	CCATC	7 cut(s)	139, 218, 381, 833, 1029, 1184, 1328
BceAI	ACGGC	2 cut(s)	21, 270
BcgI	CGANNNNNNTGC	2 cut(s)	299, 333
BciT130I	CCWGG	1 cut(s)	267
BfaI	CTAG	3 cut(s)	18, 395, 905
BfmI	CTRYAG	1 cut(s)	93
BglII	AGATCT	1 cut(s)	595
BisI	GCNGC	2 cut(s)	35, 93
BlsI	GCNGC	2 cut(s)	36, 94
BmcAI	AGTACT	4 cut(s)	109, 413, 676, 1379
Bme1390I	CCNGG	1 cut(s)	267
BmeT110I	CYCGRG	1 cut(s)	444
BmgBI	CACGTC	1 cut(s)	833
BmiI	GGNNCC	4 cut(s)	171, 1167, 1199, 1322
BmrFI	CCNGG	1 cut(s)	267
BmsI	GCATC	2 cut(s)	136, 845
BmtI	GCTAGC	1 cut(s)	908
BpiI	GAAGAC	1 cut(s)	1436
BpmI	CTGGAG	1 cut(s)	1402
Bpu10I	CCTNAGC	1 cut(s)	321
Bpu14I	TTCGAA	1 cut(s)	40
BpuEI	CTTGAG	2 cut(s)	569, 1304
Bsa29I	ATCGAT	1 cut(s)	477
BsaBI	GATNNNNATC	1 cut(s)	615
BsaJI	CCNNGG	4 cut(s)	211, 266, 689, 944
BsaXI	ACNNNNNCTCC	2 cut(s)	451, 481
Bsc4I	CCNNNNNNNGG	2 cut(s)	737, 1269
Bse1I	ACTGG	5 cut(s)	352, 861, 1141, 1375, 1385
Bse3DI	GCAATG	2 cut(s)	1333, 1356
Bse8I	GATNNNNATC	1 cut(s)	615
BseBI	CCWGG	1 cut(s)	267
BseCI	ATCGAT	1 cut(s)	477
BseDI	CCNNGG	4 cut(s)	211, 266, 689, 944
BseGI	GGATG	4 cut(s)	127, 141, 369, 699
BseJI	GATNNNNATC	1 cut(s)	615
BseLI	CCNNNNNNNGG	2 cut(s)	737, 1269
BseMI	GCAATG	2 cut(s)	1333, 1356
BseMII	CTCAG	1 cut(s)	114
BseNI	ACTGG	5 cut(s)	352, 861, 1141, 1375, 1385
BseRI	GAGGAG	2 cut(s)	94, 774
BseXI	GCAGC	2 cut(s)	21, 79
BsgI	GTGCAG	1 cut(s)	302
BshFI	GGCC	5 cut(s)	257, 393, 794, 1074, 1209
BshNI	GGYRCC	1 cut(s)	169
BshVI	ATCGAT	1 cut(s)	477
BsiHKAI	GWGCWC	1 cut(s)	555
BsiHKCI	CYCGRG	1 cut(s)	444
BslFI	GGGAC	1 cut(s)	659
BslI	CCNNNNNNNGG	2 cut(s)	737, 1269
BsmFI	GGGAC	1 cut(s)	659
BsmI	GAATGC	3 cut(s)	94, 1250, 1273
BsnI	GGCC	5 cut(s)	257, 393, 794, 1074, 1209
BsoBI	CYCGRG	1 cut(s)	444
Bsp119I	TTCGAA	1 cut(s)	40
Bsp1286I	GDGCHC	2 cut(s)	555, 658
Bsp143I	GATC	6 cut(s)	119, 474, 478, 504, 595, 1094
Bsp19I	CCATGG	1 cut(s)	689
BspACI	CCGC	4 cut(s)	167, 1134, 1324, 1347
BspANI	GGCC	5 cut(s)	257, 393, 794, 1074, 1209
BspCNI	CTCAG	1 cut(s)	115
BspDI	ATCGAT	1 cut(s)	477
BspHI	TCATGA	1 cut(s)	712
BspLI	GGNNCC	4 cut(s)	171, 1167, 1199, 1322
BspMAI	CTGCAG	1 cut(s)	97
BspOI	GCTAGC	1 cut(s)	908
BspPI	GGATC	2 cut(s)	473, 512
BspT104I	TTCGAA	1 cut(s)	40
BspT107I	GGYRCC	1 cut(s)	169
BsrDI	GCAATG	2 cut(s)	1333, 1356
BsrI	ACTGG	5 cut(s)	352, 861, 1141, 1375, 1385
BssECI	CCNNGG	4 cut(s)	211, 266, 689, 944
BssMI	GATC	6 cut(s)	119, 474, 478, 504, 595, 1094
BssNAI	GTATAC	1 cut(s)	1174
BssSI	CACGAG	1 cut(s)	103
BssT1I	CCWWGG	3 cut(s)	211, 689, 944
Bst1107I	GTATAC	1 cut(s)	1174
Bst2BI	CACGAG	1 cut(s)	103
Bst2UI	CCWGG	1 cut(s)	267
Bst4CI	ACNGT	3 cut(s)	380, 642, 1183
Bst6I	CTCTTC	1 cut(s)	557
BstBI	TTCGAA	1 cut(s)	40
BstC8I	GCNNGC	6 cut(s)	706, 906, 1016, 1020, 1072, 1264
BstDEI	CTNAG	2 cut(s)	123, 321
BstDSI	CCRYGG	1 cut(s)	689
BstEII	GGTNACC	1 cut(s)	1136
BstF5I	GGATG	4 cut(s)	127, 141, 369, 699
BstKTI	GATC	6 cut(s)	122, 477, 481, 507, 598, 1097
BstMBI	GATC	6 cut(s)	119, 474, 478, 504, 595, 1094
BstMWI	GCNNNNNNNGC	2 cut(s)	1279, 1355
BstNI	CCWGG	1 cut(s)	267
BstNSI	RCATGY	3 cut(s)	730, 968, 1018
BstPI	GGTNACC	1 cut(s)	1136
BstSCI	CCNGG	1 cut(s)	265
BstSFI	CTRYAG	1 cut(s)	93
BstV1I	GCAGC	2 cut(s)	21, 79
BstV2I	GAAGAC	1 cut(s)	1436
BstX2I	RGATCY	1 cut(s)	595
BstXI	CCANNNNNNTGG	1 cut(s)	696
BstYI	RGATCY	1 cut(s)	595
BstZ17I	GTATAC	1 cut(s)	1174
Bsu15I	ATCGAT	1 cut(s)	477
BsuRI	GGCC	5 cut(s)	257, 393, 794, 1074, 1209
BsuTUI	ATCGAT	1 cut(s)	477
BtgI	CCRYGG	1 cut(s)	689
BtrI	CACGTC	1 cut(s)	833
BtsCI	GGATG	4 cut(s)	127, 141, 369, 699
BtsI	GCAGTG	2 cut(s)	15, 1029
BtsIMutI	CAGTG	3 cut(s)	15, 376, 1029
Cac8I	GCNNGC	6 cut(s)	706, 906, 1016, 1020, 1072, 1264
CciI	TCATGA	1 cut(s)	712
ClaI	ATCGAT	1 cut(s)	477
CseI	GACGC	1 cut(s)	1364
Csp6I	GTAC	7 cut(s)	108, 291, 412, 675, 883, 1144, 1378
CviQI	GTAC	7 cut(s)	108, 291, 412, 675, 883, 1144, 1378
DdeI	CTNAG	2 cut(s)	123, 321
DpnI	GATC	6 cut(s)	121, 476, 480, 506, 597, 1096
DpnII	GATC	6 cut(s)	119, 474, 478, 504, 595, 1094
EaeI	YGGCCR	2 cut(s)	255, 1072
Eam1104I	CTCTTC	1 cut(s)	557
EarI	CTCTTC	1 cut(s)	557
Eco130I	CCWWGG	3 cut(s)	211, 689, 944
Eco147I	AGGCCT	2 cut(s)	393, 1209
Eco24I	GRGCYC	1 cut(s)	658
Eco32I	GATATC	1 cut(s)	617
Eco57I	CTGAAG	1 cut(s)	1430
Eco88I	CYCGRG	1 cut(s)	444
Eco91I	GGTNACC	1 cut(s)	1136
EcoO65I	GGTNACC	1 cut(s)	1136
EcoRII	CCWGG	1 cut(s)	265
EcoRV	GATATC	1 cut(s)	617
EcoT14I	CCWWGG	3 cut(s)	211, 689, 944
EcoT22I	ATGCAT	1 cut(s)	966
EcoT38I	GRGCYC	1 cut(s)	658
ErhI	CCWWGG	3 cut(s)	211, 689, 944
FalI	AAGNNNNNCTT	2 cut(s)	1194, 1226
FaqI	GGGAC	1 cut(s)	659
FblI	GTMKAC	1 cut(s)	1173
Fnu4HI	GCNGC	2 cut(s)	35, 93
FokI	GGATG	4 cut(s)	114, 128, 356, 706
FriOI	GRGCYC	1 cut(s)	658
Fsp4HI	GCNGC	2 cut(s)	35, 93
FspBI	CTAG	3 cut(s)	18, 395, 905
GluI	GCNGC	2 cut(s)	35, 93
GsuI	CTGGAG	1 cut(s)	1402
HaeIII	GGCC	5 cut(s)	257, 393, 794, 1074, 1209
HgaI	GACGC	1 cut(s)	1364
HincII	GTYRAC	3 cut(s)	139, 543, 928
HindII	GTYRAC	3 cut(s)	139, 543, 928
HinfI	GANTC	7 cut(s)	26, 99, 437, 660, 1104, 1228, 1390
HphI	GGTGA	4 cut(s)	199, 227, 524, 1019
Hpy166II	GTNNAC	8 cut(s)	30, 139, 459, 543, 638, 885, 928, 1174
Hpy188I	TCNGA	6 cut(s)	124, 150, 298, 525, 830, 1256
Hpy188III	TCNNGA	8 cut(s)	103, 331, 444, 446, 548, 570, 713, 757
Hpy8I	GTNNAC	8 cut(s)	30, 139, 459, 543, 638, 885, 928, 1174
Hpy99I	CGWCG	1 cut(s)	992
HpyAV	CCTTC	7 cut(s)	67, 169, 355, 383, 998, 1199, 1212
HpyCH4III	ACNGT	3 cut(s)	380, 642, 1183
HpyCH4IV	ACGT	3 cut(s)	832, 990, 1125
HpyCH4V	TGCA	6 cut(s)	95, 202, 283, 836, 964, 1250
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1279, 1355
HpyF3I	CTNAG	2 cut(s)	123, 321
HpySE526I	ACGT	3 cut(s)	832, 990, 1125
Kzo9I	GATC	6 cut(s)	119, 474, 478, 504, 595, 1094
LmnI	GCTCC	2 cut(s)	193, 1171
Lsp1109I	GCAGC	2 cut(s)	21, 79
LweI	GCATC	2 cut(s)	136, 845
MaeI	CTAG	3 cut(s)	18, 395, 905
MaeII	ACGT	3 cut(s)	832, 990, 1125
MalI	GATC	6 cut(s)	121, 476, 480, 506, 597, 1096
MboI	GATC	6 cut(s)	119, 474, 478, 504, 595, 1094
MboII	GAAGA	6 cut(s)	574, 758, 923, 1351, 1423, 1441
MflI	RGATCY	1 cut(s)	595
MhlI	GDGCHC	2 cut(s)	555, 658
MlsI	TGGCCA	1 cut(s)	1074
MluCI	AATT	7 cut(s)	42, 423, 449, 484, 717, 914, 1443
MluNI	TGGCCA	1 cut(s)	1074
MlyI	GAGTC	1 cut(s)	35
MmeI	TCCRAC	2 cut(s)	571, 1165
Mox20I	TGGCCA	1 cut(s)	1074
Mph1103I	ATGCAT	1 cut(s)	966
MscI	TGGCCA	1 cut(s)	1074
MseI	TTAA	1 cut(s)	531
MslI	CAYNNNNRTG	4 cut(s)	694, 774, 1080, 1086
Msp20I	TGGCCA	1 cut(s)	1074
MspR9I	CCNGG	1 cut(s)	267
Mva1269I	GAATGC	3 cut(s)	94, 1250, 1273
MvaI	CCWGG	1 cut(s)	267
MwoI	GCNNNNNNNGC	2 cut(s)	1279, 1355
NcoI	CCATGG	1 cut(s)	689
NdeII	GATC	6 cut(s)	119, 474, 478, 504, 595, 1094
NheI	GCTAGC	1 cut(s)	904
NlaIV	GGNNCC	4 cut(s)	171, 1167, 1199, 1322
NmuCI	GTSAC	2 cut(s)	215, 331
NsiI	ATGCAT	1 cut(s)	966
NspI	RCATGY	3 cut(s)	730, 968, 1018
NspV	TTCGAA	1 cut(s)	40
PaeI	GCATGC	1 cut(s)	1018
PaeR7I	CTCGAG	1 cut(s)	444
PagI	TCATGA	1 cut(s)	712
PceI	AGGCCT	2 cut(s)	393, 1209
PctI	GAATGC	3 cut(s)	94, 1250, 1273
PfeI	GAWTC	6 cut(s)	99, 437, 660, 1104, 1228, 1390
PflFI	GACNNNGTC	1 cut(s)	25
PkrI	GCNGC	2 cut(s)	36, 94
PleI	GAGTC	1 cut(s)	34
PpsI	GAGTC	1 cut(s)	34
PsiI	TTATAA	1 cut(s)	1292
Psp6I	CCWGG	1 cut(s)	265
PspEI	GGTNACC	1 cut(s)	1136
PspGI	CCWGG	1 cut(s)	265
PspN4I	GGNNCC	4 cut(s)	171, 1167, 1199, 1322
PstI	CTGCAG	1 cut(s)	97
PsuI	RGATCY	1 cut(s)	595
PsyI	GACNNNGTC	1 cut(s)	25
RsaI	GTAC	7 cut(s)	109, 292, 413, 676, 884, 1145, 1379
RsaNI	GTAC	7 cut(s)	108, 291, 412, 675, 883, 1144, 1378
RseI	CAYNNNNRTG	4 cut(s)	694, 774, 1080, 1086
SaqAI	TTAA	1 cut(s)	531
SatI	GCNGC	2 cut(s)	35, 93
Sau3AI	GATC	6 cut(s)	119, 474, 478, 504, 595, 1094
ScaI	AGTACT	4 cut(s)	109, 413, 676, 1379
SchI	GAGTC	1 cut(s)	35
ScrFI	CCNGG	1 cut(s)	267
SduI	GDGCHC	2 cut(s)	555, 658
SfaNI	GCATC	2 cut(s)	136, 845
SfcI	CTRYAG	1 cut(s)	93
Sfr274I	CTCGAG	1 cut(s)	444
SfuI	TTCGAA	1 cut(s)	40
SlaI	CTCGAG	1 cut(s)	444
SmiMI	CAYNNNNRTG	4 cut(s)	694, 774, 1080, 1086
SmlI	CTYRAG	3 cut(s)	444, 548, 1283
SmoI	CTYRAG	3 cut(s)	444, 548, 1283
SphI	GCATGC	1 cut(s)	1018
Sse9I	AATT	7 cut(s)	42, 423, 449, 484, 717, 914, 1443
SseBI	AGGCCT	2 cut(s)	393, 1209
SsiI	CCGC	4 cut(s)	167, 1134, 1324, 1347
SspMI	CTAG	3 cut(s)	18, 395, 905
StuI	AGGCCT	2 cut(s)	393, 1209
StyD4I	CCNGG	1 cut(s)	265
StyI	CCWWGG	3 cut(s)	211, 689, 944
TaaI	ACNGT	3 cut(s)	380, 642, 1183
TaiI	ACGT	3 cut(s)	835, 993, 1128
TaqI	TCGA	8 cut(s)	40, 118, 387, 445, 477, 658, 666, 1102
TasI	AATT	7 cut(s)	42, 423, 449, 484, 717, 914, 1443
TatI	WGTACW	5 cut(s)	107, 411, 674, 882, 1377
TfiI	GAWTC	6 cut(s)	99, 437, 660, 1104, 1228, 1390
Tru1I	TTAA	1 cut(s)	531
Tru9I	TTAA	1 cut(s)	531
TscAI	CASTG	3 cut(s)	15, 383, 1029
TseFI	GTSAC	2 cut(s)	215, 331
TseI	GCWGC	2 cut(s)	34, 92
Tsp45I	GTSAC	2 cut(s)	215, 331
TspDTI	ATGAA	8 cut(s)	103, 429, 575, 642, 652, 729, 758, 983
TspGWI	ACGGA	2 cut(s)	38, 741
TspRI	CASTG	3 cut(s)	15, 383, 1029
Tth111I	GACNNNGTC	1 cut(s)	25
XapI	RAATTY	2 cut(s)	449, 484
XceI	RCATGY	3 cut(s)	730, 968, 1018
XcmI	CCANNNNNNNNNTGG	1 cut(s)	359
XhoI	CTCGAG	1 cut(s)	444
XmiI	GTMKAC	1 cut(s)	1173
XspI	CTAG	3 cut(s)	18, 395, 905
ZrmI	AGTACT	4 cut(s)	109, 413, 676, 1379
Zsp2I	ATGCAT	1 cut(s)	966

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.