MD02G1029300.v1.1

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013518
Physical Location & Seq
Reverse (-)
2263308 .. 2273822
10515 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1029300.v1.1.491

Sequence Viewer

Length: 1191 bp
ATGAAAAGCTTCTGTATTTTTGCTCGGCTTTTTCTCATCTTCCTGGTGTTCTTCCCTCCAATTGCCTCTGTGGCGGTGCAAGCATTCACCGGAGCATATGGAATCAATTATGGAAGAATTGCAGATAACATCCCTTCTCCTGATAAAGTTGCTACTCTTCTCAGAGCAGCAAAGATAAAGAATGTCAGGATATATGATGCTGATCACAGTGTTCTCAAGGCCTTTAGCGGGACTGGGCTTGAATTAGTGGTTGGACTTCCAAATGGGTACCTGAAAGACATGAGTGCCAATGAGGATCATGCAATGGATTGGGTTAAAGAGAATGTGCAGGCATTCCTTCCCGAGACACACATCCGCGGGATTGCTGTGGGTAATGAAGTCCTAGGTGGGGGTGATTATGAATCGTGGGCAGCTCTTTTGGGCACGGTTAAAAACATCTACAATGCAACCAAGAAGCTAGGATTAACTGATGTAGTTCAGATTACGACTGCACATTCACAGGCCGTTTTTGCTAATTCTTATCCACCCTCTTCATGTATATTTACAGATACTGTTAAGCAGCAGTACATGAAGCCACTTTTGGAGTTCTTCTCAGAAATTGGGTCTCCCTTCTGTTTAAATGCTTACCCGTTCCTTGCTTACATGAGTGATCCGGAGAATATTGATATTAATTATGCTCTTTTCCAGAAAACTCAGGGGATTTATGATCCAAAAACTGACCTTCATTATGATAATATGCTCGATGCTCAGATTGATGCAGCCTATGCAGCATTGGAAGATGCTGGGTTCAAAAAGATGGAAGTTATTGTTACAGAGACAGGGTGGGCTTCACGTGGAGGTGACAATGAAGCTGCAGCTACTGTAAATAATGCAAGGACATACAATTATAATCTACGTAAAAGGCTAGCAAAGAAAAAAGGGACCCCTATGCGGCCAAAGTTTGTAGTGAAGGCATACATTTTTGCATTATTTAATGAGAACCTGAAAACTGGTGCAACCTCTGAGCGAAATTTTGGACTGTTCAAGCCTGATGGGACCATTGCATATGATATTGGGTATCATGGGCTTGTATCATCATCTGCAGATTCATTGCTTTTATCTTCAAAGCACATTGGAGCGCAAGGTTGGTCTCGTTTGCAATTCTTGATGCTATCAATTTCTTCTGCATCATTGCTTCTGTTTTTAAAATAA

Protein Analysis

397

Amino Acids

43.64

Weight (kDa)

7.66

Isoelectric Point (pI)

35.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 34 - 351 2.5e-78 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013518)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26600 AT2G26600
fragaria_vesca FvH4_1g02990
malus_domestica MD02G1029300.v1.1 MD15G1170800.v1.1
prunus_persica Prupe.7G245600_v2.0.a1
pyrus_communis pycom02g02420 pycom15g15220
rosa_chinensis RchiOBHm_Chr2g0088131
rosa_laevigata RLG00000015924
rosa_multiflora Rmu_sc0003275.1_g000047
rosa_roxburghii Rroxscaffold_2G00152940
rosa_rugosa Rorug01G0481300
rosa_samantha Rh2AG036100 Rh2BG035300 Rh2CG036500 Rh2DG035800
rosa_wichuraiana Rw2G002840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 888
Acc65I GGTACC 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 267
AccII CGCG 1 cut(s) 357
AccIII TCCGGA 1 cut(s) 652
AciI CCGC 5 cut(s) 74, 228, 355, 357, 931
AclWI GGATC 3 cut(s) 303, 644, 701
AcoI YGGCCR 1 cut(s) 932
AcsI RAATTY 1 cut(s) 1009
AcvI CACGTG 1 cut(s) 833
AfaI GTAC 2 cut(s) 269, 566
AfiI CCNNNNNNNGG 3 cut(s) 228, 388, 930
AgsI TTSAA 4 cut(s) 242, 790, 1024, 1104
AjnI CCWGG 1 cut(s) 42
AjuI GAANNNNNNNTTGG 4 cut(s) 234, 266, 563, 595
AluBI AGCT 5 cut(s) 9, 413, 457, 851, 857
AluI AGCT 5 cut(s) 9, 413, 457, 851, 857
Alw26I GTCTC 4 cut(s) 338, 609, 809, 1134
AlwI GGATC 3 cut(s) 303, 644, 701
AlwNI CAGNNNCTG 2 cut(s) 551, 860
Ama87I CYCGRG 1 cut(s) 341
Aor13HI TCCGGA 1 cut(s) 652
AoxI GGCC 3 cut(s) 219, 501, 932
ApeKI GCWGC 7 cut(s) 167, 410, 559, 758, 767, 851, 854
ApoI RAATTY 1 cut(s) 1009
AseI ATTAAT 1 cut(s) 669
Asp700I GAANNNNTTC 1 cut(s) 8
Asp718I GGTACC 1 cut(s) 267
AspA2I CCTAGG 1 cut(s) 382
AspLEI GCGC 1 cut(s) 1120
AspS9I GGNCC 2 cut(s) 921, 1035
AsuHPI GGTGA 3 cut(s) 79, 404, 851
AsuNHI GCTAGC 1 cut(s) 904
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 2 cut(s) 921, 1035
AvrII CCTAGG 1 cut(s) 382
BaeGI GKGCMC 1 cut(s) 425
BanI GGYRCC 1 cut(s) 267
BbrPI CACGTG 1 cut(s) 833
BbvI GCAGC 7 cut(s) 179, 422, 571, 770, 779, 838, 866
BccI CCATC 2 cut(s) 790, 1025
BceAI ACGGC 1 cut(s) 488
BciT130I CCWGG 1 cut(s) 44
BclI TGATCA 1 cut(s) 202
BcoDI GTCTC 4 cut(s) 338, 609, 809, 1134
BfaI CTAG 3 cut(s) 383, 458, 905
BfmI CTRYAG 2 cut(s) 852, 1080
BglI GCCNNNNNGGC 1 cut(s) 71
BisI GCNGC 8 cut(s) 168, 411, 560, 759, 768, 852, 855, 932
BlnI CCTAGG 1 cut(s) 382
BlsI GCNGC 8 cut(s) 169, 412, 561, 760, 769, 853, 856, 933
Bme1390I CCNGG 1 cut(s) 44
Bme18I GGWCC 2 cut(s) 921, 1035
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 2 cut(s) 921, 1035
BmiI GGNNCC 4 cut(s) 269, 922, 923, 1036
BmrFI CCNGG 1 cut(s) 44
BmrI ACTGGG 1 cut(s) 243
BmsI GCATC 6 cut(s) 187, 733, 745, 769, 1137, 1175
BmtI GCTAGC 1 cut(s) 908
BmuI ACTGGG 1 cut(s) 243
BplI GAGNNNNNCTC 2 cut(s) 575, 607
BpuEI CTTGAG 1 cut(s) 200
BsaAI YACGTR 2 cut(s) 833, 896
BsaBI GATNNNNATC 1 cut(s) 201
BsaI GGTCTC 2 cut(s) 609, 1134
BsaJI CCNNGG 2 cut(s) 355, 382
BsaWI WCCGGW 2 cut(s) 89, 652
Bsc4I CCNNNNNNNGG 3 cut(s) 228, 388, 930
Bse1I ACTGG 2 cut(s) 238, 994
Bse3DI GCAATG 4 cut(s) 309, 1038, 1088, 1169
Bse8I GATNNNNATC 1 cut(s) 201
BseAI TCCGGA 1 cut(s) 652
BseBI CCWGG 1 cut(s) 44
BseDI CCNNGG 2 cut(s) 355, 382
BseGI GGATG 2 cut(s) 129, 351
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 3 cut(s) 228, 388, 930
BseMI GCAATG 4 cut(s) 309, 1038, 1088, 1169
BseMII CTCAG 5 cut(s) 175, 606, 707, 761, 993
BseNI ACTGG 2 cut(s) 238, 994
BseSI GKGCMC 1 cut(s) 425
BseXI GCAGC 7 cut(s) 179, 422, 571, 770, 779, 838, 866
BseYI CCCAGC 1 cut(s) 782
BsgI GTGCAG 2 cut(s) 347, 474
Bsh1236I CGCG 1 cut(s) 357
BshFI GGCC 3 cut(s) 221, 503, 934
BshNI GGYRCC 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 2 cut(s) 90, 653
BslFI GGGAC 3 cut(s) 244, 934, 1048
BslI CCNNNNNNNGG 3 cut(s) 228, 388, 930
BsmAI GTCTC 4 cut(s) 338, 609, 809, 1134
BsmFI GGGAC 3 cut(s) 244, 934, 1048
BsmI GAATGC 2 cut(s) 83, 332
BsnI GGCC 3 cut(s) 221, 503, 934
Bso31I GGTCTC 2 cut(s) 609, 1134
BsoBI CYCGRG 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 425
Bsp13I TCCGGA 1 cut(s) 652
Bsp143I GATC 4 cut(s) 202, 295, 649, 706
BspACI CCGC 5 cut(s) 74, 228, 355, 357, 931
BspANI GGCC 3 cut(s) 221, 503, 934
BspCNI CTCAG 5 cut(s) 174, 605, 706, 760, 994
BspEI TCCGGA 1 cut(s) 652
BspFNI CGCG 1 cut(s) 357
BspLI GGNNCC 4 cut(s) 269, 922, 923, 1036
BspMAI CTGCAG 2 cut(s) 856, 1084
BspOI GCTAGC 1 cut(s) 908
BspPI GGATC 3 cut(s) 303, 644, 701
BspT107I GGYRCC 1 cut(s) 267
BspTNI GGTCTC 2 cut(s) 609, 1134
BsrDI GCAATG 4 cut(s) 309, 1038, 1088, 1169
BsrI ACTGG 2 cut(s) 238, 994
BssECI CCNNGG 2 cut(s) 355, 382
BssMI GATC 4 cut(s) 202, 295, 649, 706
BssT1I CCWWGG 1 cut(s) 382
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 5 cut(s) 209, 427, 553, 862, 1020
Bst6I CTCTTC 2 cut(s) 162, 535
BstAPI GCANNNNNTGC 1 cut(s) 764
BstBAI YACGTR 2 cut(s) 833, 896
BstC8I GCNNGC 3 cut(s) 81, 330, 906
BstDEI CTNAG 5 cut(s) 161, 592, 693, 747, 1002
BstDSI CCRYGG 1 cut(s) 355
BstF5I GGATG 2 cut(s) 129, 351
BstFNI CGCG 1 cut(s) 357
BstHHI GCGC 1 cut(s) 1120
BstKTI GATC 4 cut(s) 205, 298, 652, 709
BstMAI GTCTC 4 cut(s) 338, 609, 809, 1134
BstMBI GATC 4 cut(s) 202, 295, 649, 706
BstMWI GCNNNNNNNGC 5 cut(s) 71, 80, 509, 764, 767
BstNI CCWGG 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 42
BstSFI CTRYAG 2 cut(s) 852, 1080
BstSLI GKGCMC 1 cut(s) 425
BstSNI TACGTA 1 cut(s) 896
BstUI CGCG 1 cut(s) 357
BstV1I GCAGC 7 cut(s) 179, 422, 571, 770, 779, 838, 866
BsuRI GGCC 3 cut(s) 221, 503, 934
BtgI CCRYGG 1 cut(s) 355
BtsCI GGATG 2 cut(s) 129, 351
BtsIMutI CAGTG 1 cut(s) 214
Cac8I GCNNGC 3 cut(s) 81, 330, 906
CaiI CAGNNNCTG 2 cut(s) 551, 860
CfoI GCGC 1 cut(s) 1120
Cfr13I GGNCC 2 cut(s) 921, 1035
Cfr42I CCGCGG 1 cut(s) 358
Csp6I GTAC 2 cut(s) 268, 565
CviAII CATG 6 cut(s) 280, 299, 534, 568, 643, 1061
CviQI GTAC 2 cut(s) 268, 565
DdeI CTNAG 5 cut(s) 161, 592, 693, 747, 1002
DpnI GATC 4 cut(s) 204, 297, 651, 708
DpnII GATC 4 cut(s) 202, 295, 649, 706
DraI TTTAAA 2 cut(s) 618, 1185
EaeI YGGCCR 1 cut(s) 932
Eam1104I CTCTTC 2 cut(s) 162, 535
EarI CTCTTC 2 cut(s) 162, 535
Eco105I TACGTA 1 cut(s) 896
Eco130I CCWWGG 1 cut(s) 382
Eco147I AGGCCT 1 cut(s) 221
Eco31I GGTCTC 2 cut(s) 609, 1134
Eco47I GGWCC 2 cut(s) 921, 1035
Eco72I CACGTG 1 cut(s) 833
Eco88I CYCGRG 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 921
EcoRII CCWGG 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 382
FaeI CATG 6 cut(s) 283, 302, 537, 571, 646, 1064
FaqI GGGAC 3 cut(s) 244, 934, 1048
FatI CATG 6 cut(s) 279, 298, 533, 567, 642, 1060
FauI CCCGC 2 cut(s) 221, 350
FauNDI CATATG 2 cut(s) 97, 1045
FbaI TGATCA 1 cut(s) 202
Fnu4HI GCNGC 8 cut(s) 168, 411, 560, 759, 768, 852, 855, 932
FokI GGATG 2 cut(s) 116, 338
Fsp4HI GCNGC 8 cut(s) 168, 411, 560, 759, 768, 852, 855, 932
FspBI CTAG 3 cut(s) 383, 458, 905
GlaI GCGC 1 cut(s) 1119
GluI GCNGC 8 cut(s) 168, 411, 560, 759, 768, 852, 855, 932
GsaI CCCAGC 1 cut(s) 786
HaeIII GGCC 3 cut(s) 221, 503, 934
HapII CCGG 2 cut(s) 90, 653
HhaI GCGC 1 cut(s) 1120
Hin1II CATG 6 cut(s) 283, 302, 537, 571, 646, 1064
Hin6I GCGC 1 cut(s) 1118
HinP1I GCGC 1 cut(s) 1118
HindIII AAGCTT 1 cut(s) 7
HinfI GANTC 3 cut(s) 102, 401, 1085
HpaII CCGG 2 cut(s) 90, 653
HphI GGTGA 3 cut(s) 79, 404, 851
Hpy188I TCNGA 5 cut(s) 164, 480, 595, 750, 1003
Hpy188III TCNNGA 6 cut(s) 140, 187, 341, 653, 685, 1144
HpyAV CCTTC 5 cut(s) 144, 347, 619, 731, 943
HpyCH4III ACNGT 5 cut(s) 209, 427, 553, 862, 1020
HpyCH4IV ACGT 2 cut(s) 832, 895
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 80, 509, 764, 767
HpyF3I CTNAG 5 cut(s) 161, 592, 693, 747, 1002
HpySE526I ACGT 2 cut(s) 832, 895
Hsp92II CATG 6 cut(s) 283, 302, 537, 571, 646, 1064
HspAI GCGC 1 cut(s) 1118
KflI GGGWCCC 1 cut(s) 921
Kpn2I TCCGGA 1 cut(s) 652
KpnI GGTACC 1 cut(s) 271
Ksp22I TGATCA 1 cut(s) 202
KspI CCGCGG 1 cut(s) 358
Kzo9I GATC 4 cut(s) 202, 295, 649, 706
LmnI GCTCC 2 cut(s) 92, 1115
Lsp1109I GCAGC 7 cut(s) 179, 422, 571, 770, 779, 838, 866
LweI GCATC 6 cut(s) 187, 733, 745, 769, 1137, 1175
MaeI CTAG 3 cut(s) 383, 458, 905
MaeII ACGT 2 cut(s) 832, 895
MaeIII GTNAC 2 cut(s) 808, 839
MalI GATC 4 cut(s) 204, 297, 651, 708
MboI GATC 4 cut(s) 202, 295, 649, 706
MboII GAAGA 9 cut(s) 31, 43, 126, 149, 522, 580, 788, 1092, 1152
MfeI CAATTG 1 cut(s) 60
MhlI GDGCHC 1 cut(s) 425
MmeI TCCRAC 1 cut(s) 232
MnlI CCTC 6 cut(s) 66, 76, 286, 538, 830, 1009
MroI TCCGGA 1 cut(s) 652
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 8 cut(s) 315, 429, 464, 555, 617, 669, 972, 1184
MspA1I CMGCKG 1 cut(s) 357
MspI CCGG 2 cut(s) 90, 653
MspR9I CCNGG 1 cut(s) 44
MunI CAATTG 1 cut(s) 60
Mva1269I GAATGC 2 cut(s) 83, 332
MvaI CCWGG 1 cut(s) 44
MvnI CGCG 1 cut(s) 357
MwoI GCNNNNNNNGC 5 cut(s) 71, 80, 509, 764, 767
NdeI CATATG 2 cut(s) 97, 1045
NdeII GATC 4 cut(s) 202, 295, 649, 706
NheI GCTAGC 1 cut(s) 904
NlaIII CATG 6 cut(s) 283, 302, 537, 571, 646, 1064
NlaIV GGNNCC 4 cut(s) 269, 922, 923, 1036
NmeAIII GCCGAG 1 cut(s) 4
NmuCI GTSAC 1 cut(s) 839
PceI AGGCCT 1 cut(s) 221
PctI GAATGC 2 cut(s) 83, 332
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 3 cut(s) 102, 401, 1085
PkrI GCNGC 8 cut(s) 169, 412, 561, 760, 769, 853, 856, 933
PmaCI CACGTG 1 cut(s) 833
PmlI CACGTG 1 cut(s) 833
Ppu21I YACGTR 2 cut(s) 833, 896
PpuMI RGGWCCY 1 cut(s) 921
PshBI ATTAAT 1 cut(s) 669
PsiI TTATAA 1 cut(s) 888
Psp5II RGGWCCY 1 cut(s) 921
Psp6I CCWGG 1 cut(s) 42
PspCI CACGTG 1 cut(s) 833
PspFI CCCAGC 1 cut(s) 782
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 4 cut(s) 269, 922, 923, 1036
PspPI GGNCC 2 cut(s) 921, 1035
PspPPI RGGWCCY 1 cut(s) 921
PstI CTGCAG 2 cut(s) 856, 1084
PstNI CAGNNNCTG 2 cut(s) 551, 860
RsaI GTAC 2 cut(s) 269, 566
RsaNI GTAC 2 cut(s) 268, 565
SacII CCGCGG 1 cut(s) 358
SaqAI TTAA 8 cut(s) 315, 429, 464, 555, 617, 669, 972, 1184
SatI GCNGC 8 cut(s) 168, 411, 560, 759, 768, 852, 855, 932
Sau3AI GATC 4 cut(s) 202, 295, 649, 706
Sau96I GGNCC 2 cut(s) 921, 1035
ScrFI CCNGG 1 cut(s) 44
SduI GDGCHC 1 cut(s) 425
SfaNI GCATC 6 cut(s) 187, 733, 745, 769, 1137, 1175
SfcI CTRYAG 2 cut(s) 852, 1080
Sfr303I CCGCGG 1 cut(s) 358
SgrBI CCGCGG 1 cut(s) 358
SinI GGWCC 2 cut(s) 921, 1035
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SnaBI TACGTA 1 cut(s) 896
SseBI AGGCCT 1 cut(s) 221
SsiI CCGC 5 cut(s) 74, 228, 355, 357, 931
SspI AATATT 1 cut(s) 661
SspMI CTAG 3 cut(s) 383, 458, 905
StuI AGGCCT 1 cut(s) 221
StyD4I CCNGG 1 cut(s) 42
StyI CCWWGG 1 cut(s) 382
TaaI ACNGT 5 cut(s) 209, 427, 553, 862, 1020
TaiI ACGT 2 cut(s) 835, 898
TaqI TCGA 1 cut(s) 741
TatI WGTACW 1 cut(s) 564
TauI GCSGC 1 cut(s) 934
TfiI GAWTC 3 cut(s) 102, 401, 1085
Tru1I TTAA 8 cut(s) 315, 429, 464, 555, 617, 669, 972, 1184
Tru9I TTAA 8 cut(s) 315, 429, 464, 555, 617, 669, 972, 1184
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 1 cut(s) 839
TseI GCWGC 7 cut(s) 167, 410, 559, 758, 767, 851, 854
Tsp45I GTSAC 1 cut(s) 839
TspDTI ATGAA 8 cut(s) 17, 390, 414, 522, 584, 713, 861, 1077
TspRI CASTG 1 cut(s) 214
VpaK11BI GGWCC 2 cut(s) 921, 1035
VspI ATTAAT 1 cut(s) 669
XapI RAATTY 1 cut(s) 1009
XmaJI CCTAGG 1 cut(s) 382
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 3 cut(s) 383, 458, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.