MD02G1029500.v1.1

Thylakoid lumenal 16.5 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011404
Physical Location & Seq
Forward (+)
2280760 .. 2282750
1991 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1029500.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGCCACAGCCATTCTCTCCACTGCAAAATCCTTCCTCCCTTCATCTTCTGCTACTCCTCCTTCTTCACTGACAACAACACCAAAACTAATTCATGTCCAAAAAACAAGTACAAGGAGAGGATTGACTTTTTGTCAGGCACTGAGTGAAGAGCCGCAATCACCTGCTCCAATCCTCACTAAAAGAAGCCTGTCCATCTGCTTTCTCACCAGCTTCGCCTTCTCATTGGTTGGCAAAGATTTCAGCAGTTCTAAAGCAGCAATTCTGGAAGCTGATGATGACGAGGAACTGTTGGAGAAGGTTAAGAAGGACAGGAAGAAGAGGCTTGAAAGGCAAGGAGTTCTCAGCTCATCAAAGAAAGAAACAGGATATCTGCAGGAACTGGTGTACAGGCTGAGCAAAGCAGGCCAAGCCATTGACAGTAACGATTTGCCTGCAGCCAGTTCGGTTCTTGGGGGCAGCACTGACACAGATTGGGTTCAGAAGGCGAATATCGCTTTGAATAAGCTAAGCTCTAGCCCTGAAGAGAAGACTGAGGTGGATACATTCAACTCCTCACTAGCTTCATTGATATTATCAGTAACCCGGAACGATGTGGAGTCCTCCAAGCTTGCTTTTGTGTCTTCTGCAAGTGCAATTGAGAAATGGACGACATTAACAGGGCTCATTGGCCAGCTTAAGGGACTTTAA

Protein Analysis

230

Amino Acids

24.58

Weight (kDa)

8.78

Isoelectric Point (pI)

54.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MPH2 PF20675 124 - 229 2.8e-46 Maintenance of Photosystem II under High light 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02530 AT4G02530 AT4G02530
fragaria_vesca FvH4_1g03010
malus_domestica MD02G1029500.v1.1 MD15G1170900.v1.1
prunus_persica Prupe.7G245400_v2.0.a1
pyrus_communis pycom02g02440 pycom15g15240
rosa_chinensis RchiOBHm_Chr2g0088141
rosa_laevigata RLG00000015925
rosa_multiflora Rmu_co7966632.1_g000001 Rmu_sc0003275.1_g000049
rosa_roxburghii Rroxscaffold_2G00152930
rosa_rugosa Rorug01G0481300
rosa_samantha Rh2AG036200 Rh2BG035400 Rh2CG036600 Rh2DG035900
rosa_wichuraiana Rw2G002850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 172
Acc36I ACCTGC 1 cut(s) 172
AciI CCGC 1 cut(s) 155
AcoI YGGCCR 2 cut(s) 3, 670
AcuI CTGAAG 1 cut(s) 543
AdeI CACNNNGTG 1 cut(s) 146
AfaI GTAC 2 cut(s) 112, 389
AfiI CCNNNNNNNGG 1 cut(s) 679
AflII CTTAAG 1 cut(s) 677
AgsI TTSAA 3 cut(s) 329, 502, 550
AhdI GACNNNNNGTC 1 cut(s) 132
AluBI AGCT 8 cut(s) 213, 272, 348, 508, 513, 563, 610, 676
AluI AGCT 8 cut(s) 213, 272, 348, 508, 513, 563, 610, 676
AlwNI CAGNNNCTG 2 cut(s) 142, 382
AoxI GGCC 3 cut(s) 3, 406, 670
ApeKI GCWGC 3 cut(s) 257, 437, 459
AsuC2I CCSGG 1 cut(s) 586
AsuHPI GGTGA 2 cut(s) 153, 199
BalI TGGCCA 2 cut(s) 5, 672
BanII GRGCYC 1 cut(s) 666
BbsI GAAGAC 2 cut(s) 536, 615
BbvI GCAGC 3 cut(s) 269, 449, 471
BccI CCATC 1 cut(s) 203
BcgI CGANNNNNNTGC 4 cut(s) 416, 426, 450, 460
BciVI GTATCC 1 cut(s) 535
BcnI CCSGG 1 cut(s) 586
BfaI CTAG 2 cut(s) 516, 560
BfmI CTRYAG 2 cut(s) 374, 435
BfrI CTTAAG 1 cut(s) 677
BfuAI ACCTGC 1 cut(s) 172
BfuI GTATCC 1 cut(s) 535
BisI GCNGC 4 cut(s) 155, 258, 438, 460
BlpI GCTNAGC 2 cut(s) 395, 509
BlsI GCNGC 4 cut(s) 156, 259, 439, 461
Bme1390I CCNGG 1 cut(s) 586
BmeRI GACNNNNNGTC 1 cut(s) 132
BmrFI CCNGG 1 cut(s) 586
BpiI GAAGAC 2 cut(s) 536, 615
Bpu1102I GCTNAGC 2 cut(s) 395, 509
BpuMI CCSGG 1 cut(s) 586
Bsc4I CCNNNNNNNGG 1 cut(s) 679
Bse1I ACTGG 2 cut(s) 387, 441
BseLI CCNNNNNNNGG 1 cut(s) 679
BseMII CTCAG 4 cut(s) 134, 358, 386, 525
BseNI ACTGG 2 cut(s) 387, 441
BseRI GAGGAG 2 cut(s) 48, 544
BseXI GCAGC 3 cut(s) 269, 449, 471
BshFI GGCC 3 cut(s) 5, 408, 672
BsiSI CCGG 1 cut(s) 586
BslI CCNNNNNNNGG 1 cut(s) 679
BsnI GGCC 3 cut(s) 5, 408, 672
Bsp1286I GDGCHC 1 cut(s) 666
Bsp1407I TGTACA 1 cut(s) 387
Bsp1720I GCTNAGC 2 cut(s) 395, 509
BspACI CCGC 1 cut(s) 155
BspANI GGCC 3 cut(s) 5, 408, 672
BspCNI CTCAG 4 cut(s) 135, 357, 387, 526
BspMAI CTGCAG 2 cut(s) 378, 439
BspMI ACCTGC 1 cut(s) 172
BspQI GCTCTTC 1 cut(s) 144
BspTI CTTAAG 1 cut(s) 677
BsrGI TGTACA 1 cut(s) 387
BsrI ACTGG 2 cut(s) 387, 441
Bst4CI ACNGT 2 cut(s) 291, 422
Bst6I CTCTTC 3 cut(s) 144, 314, 519
BstAFI CTTAAG 1 cut(s) 677
BstAUI TGTACA 1 cut(s) 387
BstC8I GCNNGC 4 cut(s) 406, 435, 612, 674
BstDEI CTNAG 5 cut(s) 143, 344, 395, 509, 534
BstMWI GCNNNNNNNGC 4 cut(s) 331, 405, 410, 494
BstSCI CCNGG 1 cut(s) 584
BstSFI CTRYAG 2 cut(s) 374, 435
BstV1I GCAGC 3 cut(s) 269, 449, 471
BstV2I GAAGAC 2 cut(s) 536, 615
BsuI GTATCC 1 cut(s) 535
BsuRI GGCC 3 cut(s) 5, 408, 672
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 4 cut(s) 21, 68, 140, 462
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 4 cut(s) 406, 435, 612, 674
CaiI CAGNNNCTG 2 cut(s) 142, 382
Csp6I GTAC 2 cut(s) 111, 388
CviAII CATG 1 cut(s) 95
CviQI GTAC 2 cut(s) 111, 388
DdeI CTNAG 5 cut(s) 143, 344, 395, 509, 534
DraIII CACNNNGTG 1 cut(s) 146
DriI GACNNNNNGTC 1 cut(s) 132
EaeI YGGCCR 2 cut(s) 3, 670
Eam1104I CTCTTC 3 cut(s) 144, 314, 519
Eam1105I GACNNNNNGTC 1 cut(s) 132
EarI CTCTTC 3 cut(s) 144, 314, 519
Eco24I GRGCYC 1 cut(s) 666
Eco32I GATATC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 543
EcoRV GATATC 1 cut(s) 371
EcoT38I GRGCYC 1 cut(s) 666
FaeI CATG 1 cut(s) 98
FaiI YATR 1 cut(s) 96
FatI CATG 1 cut(s) 94
Fnu4HI GCNGC 4 cut(s) 155, 258, 438, 460
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 4 cut(s) 155, 258, 438, 460
FspBI CTAG 2 cut(s) 516, 560
GluI GCNGC 4 cut(s) 155, 258, 438, 460
HaeIII GGCC 3 cut(s) 5, 408, 672
HapII CCGG 1 cut(s) 586
Hin1II CATG 1 cut(s) 98
HindIII AAGCTT 1 cut(s) 608
HinfI GANTC 1 cut(s) 599
HpaII CCGG 1 cut(s) 586
HphI GGTGA 2 cut(s) 153, 199
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 1 cut(s) 483
Hpy188III TCNNGA 1 cut(s) 266
Hpy8I GTNNAC 1 cut(s) 388
HpyAV CCTTC 7 cut(s) 43, 51, 72, 229, 292, 301, 478
HpyCH4III ACNGT 2 cut(s) 291, 422
HpyCH4V TGCA 5 cut(s) 26, 376, 437, 629, 635
HpyF10VI GCNNNNNNNGC 4 cut(s) 331, 405, 410, 494
HpyF3I CTNAG 5 cut(s) 143, 344, 395, 509, 534
Hsp92II CATG 1 cut(s) 98
LguI GCTCTTC 1 cut(s) 144
LmnI GCTCC 1 cut(s) 172
Lsp1109I GCAGC 3 cut(s) 269, 449, 471
MaeI CTAG 2 cut(s) 516, 560
MaeIII GTNAC 2 cut(s) 422, 580
MboII GAAGA 8 cut(s) 39, 57, 161, 328, 331, 536, 541, 615
MfeI CAATTG 1 cut(s) 636
MhlI GDGCHC 1 cut(s) 666
MlsI TGGCCA 2 cut(s) 5, 672
MluCI AATT 3 cut(s) 90, 261, 636
MluNI TGGCCA 2 cut(s) 5, 672
MlyI GAGTC 1 cut(s) 608
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 9 cut(s) 47, 69, 113, 185, 277, 315, 529, 565, 613
Mox20I TGGCCA 2 cut(s) 5, 672
MscI TGGCCA 2 cut(s) 5, 672
MseI TTAA 4 cut(s) 303, 656, 678, 688
Msp20I TGGCCA 2 cut(s) 5, 672
MspCI CTTAAG 1 cut(s) 677
MspI CCGG 1 cut(s) 586
MspR9I CCNGG 1 cut(s) 586
MunI CAATTG 1 cut(s) 636
MwoI GCNNNNNNNGC 4 cut(s) 331, 405, 410, 494
NciI CCSGG 1 cut(s) 586
NlaIII CATG 1 cut(s) 98
PaqCI CACCTGC 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 144
PkrI GCNGC 4 cut(s) 156, 259, 439, 461
PleI GAGTC 1 cut(s) 607
PpsI GAGTC 1 cut(s) 607
PstI CTGCAG 2 cut(s) 378, 439
PstNI CAGNNNCTG 2 cut(s) 142, 382
RsaI GTAC 2 cut(s) 112, 389
RsaNI GTAC 2 cut(s) 111, 388
SapI GCTCTTC 1 cut(s) 144
SaqAI TTAA 4 cut(s) 303, 656, 678, 688
SatI GCNGC 4 cut(s) 155, 258, 438, 460
SchI GAGTC 1 cut(s) 608
ScrFI CCNGG 1 cut(s) 586
SduI GDGCHC 1 cut(s) 666
SfcI CTRYAG 2 cut(s) 374, 435
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
Sse9I AATT 3 cut(s) 90, 261, 636
SsiI CCGC 1 cut(s) 155
SspMI CTAG 2 cut(s) 516, 560
StyD4I CCNGG 1 cut(s) 584
TaaI ACNGT 2 cut(s) 291, 422
TasI AATT 3 cut(s) 90, 261, 636
TatI WGTACW 2 cut(s) 110, 387
TauI GCSGC 1 cut(s) 157
Tru1I TTAA 4 cut(s) 303, 656, 678, 688
Tru9I TTAA 4 cut(s) 303, 656, 678, 688
TscAI CASTG 4 cut(s) 28, 75, 147, 469
TseI GCWGC 3 cut(s) 257, 437, 459
TspDTI ATGAA 3 cut(s) 33, 83, 555
TspRI CASTG 4 cut(s) 28, 75, 147, 469
Vha464I CTTAAG 1 cut(s) 677
XspI CTAG 2 cut(s) 516, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.