MD02G1036600.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013582
Physical Location & Seq
Forward (+)
2769829 .. 2774049
4221 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1036600.v1.1.491

Sequence Viewer

Length: 2991 bp
ATGACTACCATGAAAGAGCCACCTCTCTCCCGTCGCAGAAGGCCAACTTGCCACCAGCCCAAGAAGCTGAAGCTCGTCTGCTGCTTCAACGGCGCGTTCCATCCCCGCCCGCCGTCCGGGAAGCTCCGCTACGTCGGCGGCGAGGCCAGAATCGTATCCGTGGACCGCAACATCGTGTACTCCAAGCTCCGATCAAAGATTTTGGATCTTTGCCCCAACATTAACCCCTCAGTCACCCTCGTGTACCAGCTCCCGGGTTCCTGCTCCGACTCCGACTCCGCCGCCCCTCTCGTCATCGTGGCGTCAGACGACGACGTTCGGTGCATGATCGAAGAGTACGACAAGCTGGAGCTGTACGGTAAGTCCCCGAGGCTCTGGGTCTTTGTTTGTTGTAGCAATGGCAGTGATGATGTCAATGGTTATGTAAATTGCGTGAAAGGAGTGAAATTTAGTAATGGGTTTGAGTCGGGGAGCGAACATATAGGGATCCACCACCATTTGGGTGGCGAAGCTCAGGTAGAGGAGAAGGCGGCGGTGAAGAACTTTGGGGGCGGACGGTCCGGCGATGAATCGCTGAGAAAAATGGTGCTGAAACAGCAATTGCTTGCAAAGCAATCGGCTTTTCGGCGTCGTTTTGGTGATGGTGAGAGTGATGTGGAAATGGGGTTTTTGGGTGAATCCCAAAAGGGTGAATCTTTTGATAGCAGATTAGGGACTAATTGTGGTCATAATCTAGGGAGTTCTTCGGTGGCTCGGGCTAATCCGTTGAATCCCAGAGATGGGAATTTGAGGTTGAAAAGTAAGAGTTATGGGCAGTGCTATTCGGGGAGGTCGGGTCGGGTTTTGTGCAATGGGGTTGGGAGGTCGAGTCAGGCGCCGCCTGCGAAACCAGTACCGGGTTGTAACGGTTGTTGTAGTGATGCTAAGCAGGATTATAGAAACAATGGAATGGCTGGATGTGAACAGAGAAAACATTATGCCTCGGGAGTGGGGCGTGATTCTTTGGCTGGAACCACTGTGTATCCTGTGAAGTCTTCTTATAGTGGTGGTAGGGCGTGGGGCGGCTTAAGGAGCCGGATTCGTAACCATAGGTTTGGTGTGAATGAAGCTAGAAATAAGCGGTGTTATCAGTACCATGTTTGGAACCATAATCGGAGCAACATAGCTGAGATGGGGAGCCATCGAACAAGGCAGGGAATTTCAGCACGGAAATGCTACCAAGGGCTTAGGCCAAATTTGAACATTGCGAAGCAGGAGCAGCCTGTAAGAGTATATAATCCCAGTTTATGGAGGCAATGTTCTGGTTTCTCGGAGCATGTAATGGAAGAAAGACTGAGGATGATGGGTTCTCGCTCAAAGAAAGACAGCTACTTGATAGATAATCCGAGCGAATCCTATTCAGGTAATTGTGGTGCATATACTGGTATGGGAAATCAACCTCTCGTGAATTCTGATGCCATTGAGAGTTCATCTCCATCTTTGAAGACAGTGGACATTCTGGAAGATCGCCTAACTGGTTCTAAGTTTGGGAAGTGTGAATGTCCATATCAAACTTCATATGAAAATCTTCATGGAATGCCCCGTCATTGTGAGCTTGAGAAGGAGACGCGCCTGATGGACTCTCATGCAGTTGCGAACACCTCTGGCTTTCCAAGTGAAGTGGGATGTAGTAATGGATTGGAAGGTGATAATAAGTTGCCTGATGGGGAAGCAGTCATCGACGCTTTACACAATTGCAAAAACGGCATTAATGACATACAACTTTCATGTAGCGGAGTCCCAGCTTCAGTGAGTGTTTCTTTGCATAACCTTTCACTGTCATCATCCAAAGAAGTGGAAGCTCCTCAACTCTCTTCTCATGCCACCAGTGTTGTGTCTTCTGATGTCAAACATCAATCAAAGCCTATAGATCTCATGGATGAAGGAAAATTTACCCCAGATCATCAAGTCGATGAATCCAACGGAGTTGCACCTAATCCTGCCTCCCAGAATTCAGCTAAGAAAGAGAAGGATAATGTGCATGATGAGGAGGTCCAACAGGACCCTTCATCTGGTATCACCATTGATGAAAAGGCCAACAATAAAGTAATTGGTGGAACCTCCAGTGATCTGGCTGCGTTTTGTGCTCATCAATCGACTCGAGAGCTTCAGAACATCAAAAGTTCTGACCTTGAGTTCATAAAAGAACTTGGTTCGGGAACATATGGAACCGTTTACTATGGAAAATGGAAGGGTTCTGATGTTGCTATAAAGAAGATTAAGCCAAGTTGCTTTACTGAAGACACAGTGAAGCAAGAGAGATTGCTTGCTGATTTTTGGAAGGAAGCTCGCATACTCAGCGAGCTTCACCACCCAAATATCGTGGCATTCTATGGTGTAGTGTCAGATGGACCAGTGACGGATTTGGCAACGGTGACAGAGTACATGGTGAATGGCTCCTTAAAACAAGTTTTGCAAAAGAAAGACCGGACTATTGATCGTCGAAAGAGGCTGATTATAGGAATGGATGCAGCTTTTGGTATGGAATATCTTCATGAGAAGAACATTGTCCATTTTGATCTGAAATCGCATAACTTTCTTGTGAATATGAGGGACCCTCAGCGTCCAGTGTGCAAGATTGGTGATCTGGGCTTATCAAAGATCAAGCAGAGGACACTTGTTTCTGGTGGAGTACGAGGTACCATACCGTGGATGGCTCCTGAACTTTTGAGCAGCAACAACTTGGTGACAGAAAAGGTTGATGTGTACTCATTTGGAATTGTGATGTGGGAACTCTTGACCGGGGAGGAACCATATGGACACCTGCGCTCGGAGGAAATAATAGCTGGTATAATAAAAGGCAGTCTCCGACCTGAAATCCCAAGCTGGTGCGATCCAACATGGAGATCGTTAATGGAAAGGTGCTGGTCATCTGATCCCAACTCTAGGCCAACCTTCTCAGAAATTGCAAAGGAGCTACGCGCCATGTCAGCTGCCATGAACATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

997

Amino Acids

109.85

Weight (kDa)

8.58

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 39 - 128 9.7e-10 PB1 domain
PK_Tyr_Ser-Thr PF07714 724 - 986 1.4e-64 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 725 - 984 1.1e-49 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013582)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03810
malus_domestica MD02G1036600.v1.1 MD15G1176700.v1.1
prunus_persica Prupe.7G238800_v2.0.a1
pyrus_communis pycom02g03010 pycom15g15820
rosa_chinensis RchiOBHm_Chr2g0089211
rosa_laevigata RLG00000016005
rosa_multiflora Rmu_sc0008322.1_g000024 Rmu_sc0030891.1_g000001
rosa_roxburghii Rroxscaffold_2G00152140
rosa_rugosa Rorug01G0488400 Rorug01G0488500
rosa_samantha Rh2AG044100 Rh2BG042800 Rh2CG044300 Rh2DG043100
rosa_wichuraiana Rw2G003900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2811
Acc36I ACCTGC 1 cut(s) 2811
Acc65I GGTACC 1 cut(s) 2678
AccB1I GGYRCC 2 cut(s) 874, 2678
AccB7I CCANNNNNTGG 3 cut(s) 499, 1287, 2688
AccII CGCG 3 cut(s) 95, 1609, 2961
AclWI GGATC 5 cut(s) 213, 481, 494, 2867, 2909
AcsI RAATTY 7 cut(s) 446, 784, 1197, 1234, 1447, 1928, 1990
AcuI CTGAAG 4 cut(s) 89, 1770, 2132, 2298
AcyI GRCGYC 3 cut(s) 302, 628, 875
AflII CTTAAG 1 cut(s) 1066
AgsI TTSAA 5 cut(s) 88, 769, 796, 1240, 1483
AleI CACNNNNGTG 1 cut(s) 239
AloI GAACNNNNNNTCC 4 cut(s) 1282, 1314, 1330, 1362
Alw21I GWGCWC 1 cut(s) 2128
Alw26I GTCTC 2 cut(s) 1598, 2849
AlwI GGATC 5 cut(s) 213, 481, 494, 2867, 2909
Ama87I CYCGRG 5 cut(s) 253, 367, 753, 982, 2139
AoxI GGCC 5 cut(s) 41, 144, 1229, 2073, 2927
ApeKI GCWGC 6 cut(s) 81, 1258, 2114, 2510, 2712, 2972
ApoI RAATTY 7 cut(s) 446, 784, 1197, 1234, 1447, 1928, 1990
AseI ATTAAT 1 cut(s) 1749
Asp700I GAANNNNTTC 2 cut(s) 1566, 2529
Asp718I GGTACC 1 cut(s) 2678
AspLEI GCGC 5 cut(s) 95, 877, 1611, 2808, 2963
AspS9I GGNCC 6 cut(s) 163, 558, 2032, 2041, 2390, 2593
AsuC2I CCSGG 5 cut(s) 118, 254, 255, 897, 2782
AvaI CYCGRG 5 cut(s) 253, 367, 753, 982, 2139
AvaII GGWCC 6 cut(s) 163, 558, 2032, 2041, 2390, 2593
BamHI GGATCC 1 cut(s) 486
BanI GGYRCC 2 cut(s) 874, 2678
BarI GAAGNNNNNNTAC 4 cut(s) 113, 145, 2316, 2348
BauI CACGAG 2 cut(s) 239, 1442
BbsI GAAGAC 4 cut(s) 1026, 1490, 1869, 2286
Bbv12I GWGCWC 1 cut(s) 2128
BbvCI CCTCAGC 1 cut(s) 2598
BbvI GCAGC 6 cut(s) 68, 1270, 2101, 2522, 2724, 2959
BceAI ACGGC 3 cut(s) 97, 106, 1759
BcgI CGANNNNNNTGC 2 cut(s) 597, 631
BciVI GTATCC 2 cut(s) 166, 1032
BcnI CCSGG 5 cut(s) 118, 254, 255, 897, 2782
BcoDI GTCTC 2 cut(s) 1598, 2849
BfaI CTAG 3 cut(s) 734, 1110, 2925
BfmI CTRYAG 1 cut(s) 1905
BfoI RGCGCY 1 cut(s) 878
BfrI CTTAAG 1 cut(s) 1066
BfuAI ACCTGC 1 cut(s) 2811
BfuI GTATCC 2 cut(s) 166, 1032
BglII AGATCT 1 cut(s) 1909
BlpI GCTNAGC 1 cut(s) 924
Bme1390I CCNGG 5 cut(s) 118, 254, 255, 897, 2782
Bme18I GGWCC 6 cut(s) 163, 558, 2032, 2041, 2390, 2593
BmeT110I CYCGRG 5 cut(s) 253, 367, 753, 982, 2139
BmgT120I GGNCC 6 cut(s) 163, 558, 2032, 2041, 2390, 2593
BmrFI CCNGG 5 cut(s) 118, 254, 255, 897, 2782
BmrI ACTGGG 1 cut(s) 1275
BmsI GCATC 3 cut(s) 910, 1444, 2497
BmuI ACTGGG 1 cut(s) 1275
BpiI GAAGAC 4 cut(s) 1026, 1490, 1869, 2286
BplI GAGNNNNNCTC 4 cut(s) 1456, 1488, 2581, 2613
BpmI CTGGAG 2 cut(s) 368, 2086
Bpu10I CCTNAGC 3 cut(s) 513, 1226, 2598
Bpu1102I GCTNAGC 1 cut(s) 924
BpuEI CTTGAG 2 cut(s) 1616, 2192
BpuMI CCSGG 5 cut(s) 118, 254, 255, 897, 2782
BsaHI GRCGYC 3 cut(s) 302, 628, 875
BsaJI CCNNGG 7 cut(s) 159, 253, 368, 981, 1219, 2687, 2781
BsaWI WCCGGW 1 cut(s) 2466
BsaXI ACNNNNNCTCC 8 cut(s) 260, 290, 820, 850, 853, 883, 1282, 1312
Bse1I ACTGG 8 cut(s) 890, 1281, 1426, 1519, 1866, 2103, 2393, 2606
Bse3DI GCAATG 4 cut(s) 403, 856, 1242, 1301
BseDI CCNNGG 7 cut(s) 159, 253, 368, 981, 1219, 2687, 2781
BseGI GGATG 8 cut(s) 100, 962, 1344, 1670, 1823, 1924, 2512, 2697
BseMI GCAATG 4 cut(s) 403, 856, 1242, 1301
BseMII CTCAG 8 cut(s) 243, 527, 566, 1158, 1325, 2350, 2612, 2952
BseNI ACTGG 8 cut(s) 890, 1281, 1426, 1519, 1866, 2103, 2393, 2606
BseRI GAGGAG 3 cut(s) 536, 1833, 2042
BseXI GCAGC 6 cut(s) 68, 1270, 2101, 2522, 2724, 2959
BseYI CCCAGC 1 cut(s) 1780
Bsh1236I CGCG 3 cut(s) 95, 1609, 2961
BshFI GGCC 5 cut(s) 43, 146, 1231, 2075, 2929
BshNI GGYRCC 2 cut(s) 874, 2678
BsiHKAI GWGCWC 1 cut(s) 2128
BsiHKCI CYCGRG 5 cut(s) 253, 367, 753, 982, 2139
BsiSI CCGG 7 cut(s) 117, 254, 561, 896, 1075, 2467, 2781
BslFI GGGAC 4 cut(s) 349, 727, 1763, 2606
BsmAI GTCTC 2 cut(s) 1598, 2849
BsmBI CGTCTC 1 cut(s) 1598
BsmFI GGGAC 4 cut(s) 349, 727, 1763, 2606
BsmI GAATGC 2 cut(s) 1581, 2366
BsnI GGCC 5 cut(s) 43, 146, 1231, 2075, 2929
BsoBI CYCGRG 5 cut(s) 253, 367, 753, 982, 2139
Bsp1286I GDGCHC 1 cut(s) 2128
Bsp1720I GCTNAGC 1 cut(s) 924
BspANI GGCC 5 cut(s) 43, 146, 1231, 2075, 2929
BspCNI CTCAG 8 cut(s) 242, 526, 567, 1159, 1326, 2349, 2611, 2951
BspFNI CGCG 3 cut(s) 95, 1609, 2961
BspHI TCATGA 1 cut(s) 2533
BspMI ACCTGC 1 cut(s) 2811
BspPI GGATC 5 cut(s) 213, 481, 494, 2867, 2909
BspT107I GGYRCC 2 cut(s) 874, 2678
BspTI CTTAAG 1 cut(s) 1066
BsrDI GCAATG 4 cut(s) 403, 856, 1242, 1301
BsrI ACTGG 8 cut(s) 890, 1281, 1426, 1519, 1866, 2103, 2393, 2606
BssECI CCNNGG 7 cut(s) 159, 253, 368, 981, 1219, 2687, 2781
BssNI GRCGYC 3 cut(s) 302, 628, 875
BssSI CACGAG 2 cut(s) 239, 1442
BssT1I CCWWGG 1 cut(s) 1219
Bst2BI CACGAG 2 cut(s) 239, 1442
Bst6I CTCTTC 2 cut(s) 327, 1858
BstACI GRCGYC 3 cut(s) 302, 628, 875
BstAFI CTTAAG 1 cut(s) 1066
BstC8I GCNNGC 6 cut(s) 110, 606, 882, 2307, 2329, 2342
BstDSI CCRYGG 2 cut(s) 159, 2687
BstF5I GGATG 8 cut(s) 100, 962, 1344, 1670, 1823, 1924, 2512, 2697
BstFNI CGCG 3 cut(s) 95, 1609, 2961
BstH2I RGCGCY 1 cut(s) 878
BstHHI GCGC 5 cut(s) 95, 877, 1611, 2808, 2963
BstMAI GTCTC 2 cut(s) 1598, 2849
BstNSI RCATGY 1 cut(s) 1319
BstSCI CCNGG 5 cut(s) 116, 252, 253, 895, 2780
BstSFI CTRYAG 1 cut(s) 1905
BstUI CGCG 3 cut(s) 95, 1609, 2961
BstV1I GCAGC 6 cut(s) 68, 1270, 2101, 2522, 2724, 2959
BstV2I GAAGAC 4 cut(s) 1026, 1490, 1869, 2286
BstX2I RGATCY 3 cut(s) 205, 486, 1909
BstXI CCANNNNNNTGG 4 cut(s) 503, 1094, 1834, 2110
BstYI RGATCY 3 cut(s) 205, 486, 1909
BsuI GTATCC 2 cut(s) 166, 1032
BsuRI GGCC 5 cut(s) 43, 146, 1231, 2075, 2929
BtgI CCRYGG 2 cut(s) 159, 2687
BtgZI GCGATG 1 cut(s) 579
BtsCI GGATG 8 cut(s) 100, 962, 1344, 1670, 1823, 1924, 2512, 2697
BtsI GCAGTG 2 cut(s) 409, 821
BveI ACCTGC 1 cut(s) 2811
Cac8I GCNNGC 6 cut(s) 110, 606, 882, 2307, 2329, 2342
CciI TCATGA 1 cut(s) 2533
CfoI GCGC 5 cut(s) 95, 877, 1611, 2808, 2963
Cfr13I GGNCC 6 cut(s) 163, 558, 2032, 2041, 2390, 2593
Cfr9I CCCGGG 1 cut(s) 253
CpoI CGGWCCG 1 cut(s) 558
CseI GACGC 5 cut(s) 291, 617, 1615, 1730, 2591
CspCI CAANNNNNGTGG 6 cut(s) 1641, 1676, 1852, 1887, 2343, 2378
CspI CGGWCCG 1 cut(s) 558
DinI GGCGCC 1 cut(s) 876
Eam1104I CTCTTC 2 cut(s) 327, 1858
EarI CTCTTC 2 cut(s) 327, 1858
EciI GGCGGA 2 cut(s) 268, 567
Eco130I CCWWGG 1 cut(s) 1219
Eco47I GGWCC 6 cut(s) 163, 558, 2032, 2041, 2390, 2593
Eco57I CTGAAG 4 cut(s) 89, 1770, 2132, 2298
Eco88I CYCGRG 5 cut(s) 253, 367, 753, 982, 2139
EcoO109I RGGNCCY 2 cut(s) 2041, 2593
EcoRI GAATTC 2 cut(s) 1447, 1990
EcoT14I CCWWGG 1 cut(s) 1219
EgeI GGCGCC 1 cut(s) 876
EheI GGCGCC 1 cut(s) 876
ErhI CCWWGG 1 cut(s) 1219
Esp3I CGTCTC 1 cut(s) 1598
FalI AAGNNNNNCTT 2 cut(s) 31, 63
FaqI GGGAC 4 cut(s) 349, 727, 1763, 2606
FauI CCCGC 2 cut(s) 113, 117
FauNDI CATATG 3 cut(s) 1558, 2203, 2794
FokI GGATG 8 cut(s) 87, 969, 1351, 1677, 1810, 1931, 2519, 2704
FspBI CTAG 3 cut(s) 734, 1110, 2925
GlaI GCGC 5 cut(s) 94, 876, 1610, 2807, 2962
GsaI CCCAGC 1 cut(s) 1784
GsuI CTGGAG 2 cut(s) 368, 2086
HaeII RGCGCY 1 cut(s) 878
HaeIII GGCC 5 cut(s) 43, 146, 1231, 2075, 2929
HapII CCGG 7 cut(s) 117, 254, 561, 896, 1075, 2467, 2781
HgaI GACGC 5 cut(s) 291, 617, 1615, 1730, 2591
HhaI GCGC 5 cut(s) 95, 877, 1611, 2808, 2963
Hin1I GRCGYC 3 cut(s) 302, 628, 875
Hin6I GCGC 5 cut(s) 93, 875, 1609, 2806, 2961
HinP1I GCGC 5 cut(s) 93, 875, 1609, 2806, 2961
HpaII CCGG 7 cut(s) 117, 254, 561, 896, 1075, 2467, 2781
Hpy166II GTNNAC 7 cut(s) 163, 178, 244, 962, 1492, 2215, 2746
Hpy188III TCNNGA 8 cut(s) 984, 1444, 1499, 2141, 2196, 2534, 2699, 2776
Hpy8I GTNNAC 7 cut(s) 163, 178, 244, 962, 1492, 2215, 2746
Hpy99I CGWCG 7 cut(s) 36, 137, 314, 317, 633, 1724, 2484
HpyCH4IV ACGT 2 cut(s) 132, 315
HpySE526I ACGT 2 cut(s) 132, 315
Hsp92I GRCGYC 3 cut(s) 302, 628, 875
HspAI GCGC 5 cut(s) 93, 875, 1609, 2806, 2961
KasI GGCGCC 1 cut(s) 874
KflI GGGWCCC 1 cut(s) 2593
KpnI GGTACC 1 cut(s) 2682
Lsp1109I GCAGC 6 cut(s) 68, 1270, 2101, 2522, 2724, 2959
LweI GCATC 3 cut(s) 910, 1444, 2497
MaeI CTAG 3 cut(s) 734, 1110, 2925
MaeII ACGT 2 cut(s) 132, 315
MaeIII GTNAC 6 cut(s) 232, 902, 1082, 2395, 2413, 2725
MfeI CAATTG 2 cut(s) 599, 1732
MflI RGATCY 3 cut(s) 205, 486, 1909
MhlI GDGCHC 1 cut(s) 2128
Mly113I GGCGCC 1 cut(s) 875
MlyI GAGTC 7 cut(s) 263, 269, 473, 877, 1613, 1785, 2131
MmeI TCCRAC 6 cut(s) 291, 297, 1983, 2059, 2872, 2900
MroXI GAANNNNTTC 2 cut(s) 1566, 2529
MseI TTAA 6 cut(s) 222, 1067, 1749, 2259, 2441, 2891
MslI CAYNNNNRTG 3 cut(s) 239, 501, 1210
MspA1I CMGCKG 1 cut(s) 2972
MspCI CTTAAG 1 cut(s) 1066
MspI CCGG 7 cut(s) 117, 254, 561, 896, 1075, 2467, 2781
MspR9I CCNGG 5 cut(s) 118, 254, 255, 897, 2782
MunI CAATTG 2 cut(s) 599, 1732
Mva1269I GAATGC 2 cut(s) 1581, 2366
MvnI CGCG 3 cut(s) 95, 1609, 2961
NarI GGCGCC 1 cut(s) 875
NciI CCSGG 5 cut(s) 118, 254, 255, 897, 2782
NdeI CATATG 3 cut(s) 1558, 2203, 2794
NmuCI GTSAC 4 cut(s) 232, 2395, 2413, 2725
NspI RCATGY 1 cut(s) 1319
OliI CACNNNNGTG 1 cut(s) 239
PaeR7I CTCGAG 1 cut(s) 2139
PagI TCATGA 1 cut(s) 2533
PaqCI CACCTGC 1 cut(s) 2811
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 336
PctI GAATGC 2 cut(s) 1581, 2366
PdmI GAANNNNTTC 2 cut(s) 1566, 2529
PfeI GAWTC 9 cut(s) 150, 569, 677, 692, 769, 998, 1078, 1391, 1955
PflMI CCANNNNNTGG 3 cut(s) 499, 1287, 2688
PfoI TCCNGGA 1 cut(s) 116
PleI GAGTC 7 cut(s) 263, 269, 472, 876, 1613, 1784, 2131
PluTI GGCGCC 1 cut(s) 878
PpsI GAGTC 7 cut(s) 263, 269, 472, 876, 1613, 1784, 2131
PpuMI RGGWCCY 2 cut(s) 2041, 2593
PshBI ATTAAT 1 cut(s) 1749
Psp5II RGGWCCY 2 cut(s) 2041, 2593
PspFI CCCAGC 1 cut(s) 1780
PspPI GGNCC 6 cut(s) 163, 558, 2032, 2041, 2390, 2593
PspPPI RGGWCCY 2 cut(s) 2041, 2593
PsuI RGATCY 3 cut(s) 205, 486, 1909
PvuII CAGCTG 1 cut(s) 2972
RseI CAYNNNNRTG 3 cut(s) 239, 501, 1210
Rsr2I CGGWCCG 1 cut(s) 558
RsrII CGGWCCG 1 cut(s) 558
SaqAI TTAA 6 cut(s) 222, 1067, 1749, 2259, 2441, 2891
Sau96I GGNCC 6 cut(s) 163, 558, 2032, 2041, 2390, 2593
SchI GAGTC 7 cut(s) 263, 269, 473, 877, 1613, 1785, 2131
ScrFI CCNGG 5 cut(s) 118, 254, 255, 897, 2782
SduI GDGCHC 1 cut(s) 2128
SfaNI GCATC 3 cut(s) 910, 1444, 2497
SfcI CTRYAG 1 cut(s) 1905
SfoI GGCGCC 1 cut(s) 876
Sfr274I CTCGAG 1 cut(s) 2139
SinI GGWCC 6 cut(s) 163, 558, 2032, 2041, 2390, 2593
SlaI CTCGAG 1 cut(s) 2139
SmaI CCCGGG 1 cut(s) 255
SmiMI CAYNNNNRTG 3 cut(s) 239, 501, 1210
SmlI CTYRAG 4 cut(s) 1066, 1595, 2139, 2171
SmoI CTYRAG 4 cut(s) 1066, 1595, 2139, 2171
SspDI GGCGCC 1 cut(s) 874
SspMI CTAG 3 cut(s) 734, 1110, 2925
StyD4I CCNGG 5 cut(s) 116, 252, 253, 895, 2780
StyI CCWWGG 1 cut(s) 1219
TaiI ACGT 2 cut(s) 135, 318
TaqI TCGA 8 cut(s) 330, 866, 1183, 1719, 1950, 2135, 2140, 2482
TatI WGTACW 3 cut(s) 177, 2421, 2745
TauI GCSGC 5 cut(s) 141, 284, 533, 880, 1065
TfiI GAWTC 9 cut(s) 150, 569, 677, 692, 769, 998, 1078, 1391, 1955
Tru1I TTAA 6 cut(s) 222, 1067, 1749, 2259, 2441, 2891
Tru9I TTAA 6 cut(s) 222, 1067, 1749, 2259, 2441, 2891
TseFI GTSAC 4 cut(s) 232, 2395, 2413, 2725
TseI GCWGC 6 cut(s) 81, 1258, 2114, 2510, 2712, 2972
Tsp45I GTSAC 4 cut(s) 232, 2395, 2413, 2725
TspGWI ACGGA 5 cut(s) 148, 753, 1222, 1977, 2414
TspMI CCCGGG 1 cut(s) 253
Van91I CCANNNNNTGG 3 cut(s) 499, 1287, 2688
Vha464I CTTAAG 1 cut(s) 1066
VpaK11BI GGWCC 6 cut(s) 163, 558, 2032, 2041, 2390, 2593
VspI ATTAAT 1 cut(s) 1749
XapI RAATTY 7 cut(s) 446, 784, 1197, 1234, 1447, 1928, 1990
XceI RCATGY 1 cut(s) 1319
XcmI CCANNNNNNNNNTGG 2 cut(s) 500, 2689
XhoI CTCGAG 1 cut(s) 2139
XmaI CCCGGG 1 cut(s) 253
XmnI GAANNNNTTC 2 cut(s) 1566, 2529
XspI CTAG 3 cut(s) 734, 1110, 2925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.