Rh2DG043100

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013582
Physical Location & Seq
Forward (+)
2987192 .. 2991542
4351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG043100.1

Sequence Viewer

Length: 2913 bp
ATGACCTCCATGAAAGAACCACCTCTCTCTCGTGGCAAAAAGCCCACTACTACCAACCACACCCAGCAGCCCAAGAAGCTCAAGCTCGTCTGCAGCTTCAACGGCGCATTCCACCCCCGGCCGCCATCCGGGAAGCTCCGCTACATCGGCGGCGAGACCCGGATCATCTCCGTCGACCGGAACGTCGGGTTCTCCAAGCTCCGGTCGAAGATTGCCGGTCTCTCCCCCAACATTGCCTCCTTCGTCATCAAGTACCAGCTCCCCGACTTCGGATCGAGCTCCGGCGCCGCCCCGCTCGTCCACGTCGACTCCGACGACGACGTCCGACGCATGGTGGAAGCGTTCGAGAAGCTCGAGAGCTACGGTAAGTCCGCCAGACTCTGGCTCTTTGTTTGTAGCAATAGCAATGGATATAGTAACGGAGTCGGACCGGGTCGTGGGGTTGGGTCAGAGACCCGGACCCGGGTGGAGGGTGAGAAGGCGGCGGTTAAGAAACACGGCGATGAATCGCTGAGGAAGATGGTGTTGAAGCAGCAATTGCTGGCCAAGCAATCGGGTTTGGTTCGTGGGTTTGGTTTTAGTGAGAGTGATATGGAAGTTGGTGGCTTGGGTGAGGCTAGGAAACGTGAGGTGTATGAACCTCTGCTTATAGATTTAGACCCTGATCGTGATGAACCGAAAATGGGGGAGTTGAATTCTGGCAACGTGGCTCAGTTTCCAGTGGCTAGGTCTAATCCATTGAACCTTAGAGATGTGAATTTGAGGGTTGATCAAGCTTTGGGGAGGTCTGGTCAGGTTTTGTGTAATGGGGTTGGGGTGGCTGATCAGTCAGTCTCGGCAGTACAGGGTTTGGAACCGGTGAATTACTCTGGTGTGAAGCCGGGGTGTAATGGTTGTGATGTTAAGAAGGATGCGGGGAAGAATGGAATTGCTAACGTGAACAGAGAGAACATTATGCCTTGGGGAGCCAGTTGTAATTTAGTGAAGGATGGTTTGAGTTGTCATTCTTGTGGTGATTCTCAGCTGGAAACTGTGAATCCGTTGTATGCCGGTGATAGGGTGTGTGGTGGCTTAAATAGCAGCATTAGGAACCATAGGTTTGGTGCGAATGATACTAGACATCAGCGGTATAATCCATTTTGTGTTAGGAACCATAAGAGCAACACAGCTGAGATGGGGAACCATCTAACTGTTAGATTGGATGGGAGGCTTTCGACAAGGAAATGCTTCCTTGGACTTAGGCCACATATGAGCATTGCAAAGCAGGGGCAGTCTGTGAGAGTGTATCACCCCAATTCATGGAGGCATTATGCTGGTTTTTCAGAGGATGCATTGGCAGAAAGGGTGAGGATGATGAATTCTAGCTCAAACAAAGACAGGTCCTTGAGTGGTGTCCAACATACTAATCAGAAGCGAATTAAAGAGTATGGCCCTGGACTTTTACAGCACATGAGTGTGAATCCCAAGGAATATTTCTTAGGTTATTATGGTGCGTTTACTAGAGTGGGTGATCAATCACTCTTGAACTGTGATGTTACTGACAATTTGTTTCCATCTGTGAGGACTATGGACACTCAGGAAAATCTCGTAACTGTTTCTAGTTTTGGAAAGTACGAAAGTGTTCATGGAGTGAGCGGCAAATGCGAGCCTATTCATCGTGATCTTGACAAGGAGACATGCCTGAAAGACATTGTGAACGGTCGTAACACCTCTGGCACTATTGATAATAAGTTGCCTAATGAGGAAACAGTCATCAATTCTTCAGGCAATTCTAAAAATGGCAGTAATGACATGCAAGATTCATATAATGGAGTAACATGTTCAGTGAATATTTCTTTGCGTAACCTTTCACTGTCCAAAGAAGTGGAAGGTCCTCAGCTCTCTTCTCATGCTAGCTGTGTGGTGACTTCTGATACCTTGCTAAAACCTCAAGATCTCAGGGATGAAGGACAATTTATCTTGGGTCCTCAAGTCAAGCAATCAAATAGAGTTGCATCTAATCCTTCATCCCACAATTCAGCTAAGATAGAGAAATGCAGTGTGCATGTTGAGGAGGTCCGACAGGACCCTTCATCTAGTTTAAGCTTCACCGAGGAGGCCAACCATGGAGAAAGTCTTCATTACTCTAAAGTAATTGGTGGAACCTCCAACGATCTGGCTGCACTTTATACTCATCTAGCCACTCGAGAGCTACAGACTATCAAAAACTCTGACCTTGAGTTTATAAAAGAGCTTGGTTCGGGCACATATGGAACAGTTTACCATGGGAAATGGAAGGGGTCTAATGTTGCCATAAAAAAGATCAAGCCAAGTTGCTTTACAGATGGCGTATTGGAGGATCGATTGCTTGCTGACTTTTGGAAGGAAGCTCATATCTTAGGTCAACTTCACCATCCAAACGTTGTGGCATTCTATGGTGTAGTGACAGATGGACCAGTGACAAATTTGGCAACGGTGACAGAGTACATGGTGAATGGCTCCTTAAAACAAGTTTTGCGAAGGAAAGATCGGACTATTGATCGTAGGAAGAGGTTGATATTAGCAATGGATGCAGCCTTTGGTATGGAATATCTTCACGAGAAGAGCATAGTCCATTTTGATTTGAAATCACATAACTTTCTAGTGAATATGAGGGATCCTCAGCGACCAGTGTGCAAGGTTGATGTATATTCATTTGGAATTGTTATGTGGGAACTGTTGACTGGGGAGGAGCCTTACGCAAACTTGCGCTCAGAGGAAATAATAGTTGGTATAATCAAAGGCAGTCTTCGTCCTGAAATACCAAGCTGGTGTGATCCAACATGGAAATCATTGATGGAGAGATGTTGGTCATCTGATCCAGAATCTAGGCCGCCCTTCTCAGAAATCTCAAAGGAGTTGCGTGCAATGTCTGCTGCTATGAACATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

970

Amino Acids

107.19

Weight (kDa)

8.79

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 43 - 130 6e-08 PB1 domain
PK_Tyr_Ser-Thr PF07714 739 - 877 1e-29 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 740 - 877 8.1e-24 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 886 - 960 1.5e-17 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 886 - 958 2.8e-07 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013582)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03810
malus_domestica MD02G1036600.v1.1 MD15G1176700.v1.1
prunus_persica Prupe.7G238800_v2.0.a1
pyrus_communis pycom02g03010 pycom15g15820
rosa_chinensis RchiOBHm_Chr2g0089211
rosa_laevigata RLG00000016005
rosa_multiflora Rmu_sc0008322.1_g000024 Rmu_sc0030891.1_g000001
rosa_roxburghii Rroxscaffold_2G00152140
rosa_rugosa Rorug01G0488400 Rorug01G0488500
rosa_samantha Rh2AG044100 Rh2BG042800 Rh2CG044300 Rh2DG043100
rosa_wichuraiana Rw2G003900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2225
AasI GACNNNNNNGTC 2 cut(s) 182, 320
AatII GACGTC 1 cut(s) 324
AccB1I GGYRCC 1 cut(s) 284
AccB7I CCANNNNNTGG 2 cut(s) 381, 1299
AccBSI CCGCTC 2 cut(s) 295, 1635
AccI GTMKAC 2 cut(s) 174, 306
AclI AACGTT 1 cut(s) 2400
AclWI GGATC 7 cut(s) 170, 280, 2346, 2630, 2643, 2789, 2831
AcoI YGGCCR 2 cut(s) 119, 543
AcsI RAATTY 4 cut(s) 694, 757, 1357, 2443
AcuI CTGAAG 1 cut(s) 1746
AcyI GRCGYC 2 cut(s) 285, 321
AfaI GTAC 4 cut(s) 254, 843, 1613, 2465
AflIII ACRYGT 1 cut(s) 1817
AgeI ACCGGT 1 cut(s) 856
AgsI TTSAA 6 cut(s) 100, 529, 694, 742, 1525, 2605
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 1 cut(s) 1432
AjuI GAANNNNNNNTTGG 4 cut(s) 1457, 1489, 2730, 2762
AleI CACNNNNGTG 1 cut(s) 1452
Alw21I GWGCWC 1 cut(s) 281
Alw26I GTCTC 5 cut(s) 149, 224, 446, 838, 1667
AlwI GGATC 7 cut(s) 170, 280, 2346, 2630, 2643, 2789, 2831
AlwNI CAGNNNCTG 1 cut(s) 381
Ama87I CYCGRG 3 cut(s) 353, 462, 2184
AoxI GGCC 6 cut(s) 119, 543, 1241, 1429, 2097, 2849
ApeKI GCWGC 7 cut(s) 67, 93, 532, 1080, 2159, 2552, 2894
ApoI RAATTY 4 cut(s) 694, 757, 1357, 2443
AsiGI ACCGGT 1 cut(s) 856
Asp700I GAANNNNTTC 4 cut(s) 1226, 1620, 2115, 2571
AspLEI GCGC 3 cut(s) 107, 287, 2730
AspS9I GGNCC 9 cut(s) 428, 459, 1380, 1430, 1871, 1964, 2056, 2065, 2432
AsuC2I CCSGG 8 cut(s) 118, 130, 160, 432, 457, 463, 464, 882
AsuNHI GCTAGC 1 cut(s) 1892
AvaI CYCGRG 3 cut(s) 353, 462, 2184
AvaII GGWCC 8 cut(s) 428, 459, 1380, 1871, 1964, 2056, 2065, 2432
BaeGI GKGCMC 1 cut(s) 2246
BalI TGGCCA 1 cut(s) 545
BamHI GGATCC 1 cut(s) 2635
BanI GGYRCC 1 cut(s) 284
BanII GRGCYC 1 cut(s) 281
BarI GAAGNNNNNNTAC 2 cut(s) 125, 157
BauI CACGAG 2 cut(s) 30, 2576
BbsI GAAGAC 2 cut(s) 2108, 2759
Bbv12I GWGCWC 1 cut(s) 281
BbvCI CCTCAGC 3 cut(s) 512, 1875, 2640
BbvI GCAGC 7 cut(s) 79, 105, 544, 1092, 2146, 2564, 2881
BceAI ACGGC 2 cut(s) 118, 514
BcgI CGANNNNNNTGC 4 cut(s) 2141, 2175, 2634, 2668
BciT130I CCWGG 1 cut(s) 1434
BclI TGATCA 3 cut(s) 769, 823, 1510
BcnI CCSGG 8 cut(s) 118, 130, 160, 432, 457, 463, 464, 882
BcoDI GTCTC 5 cut(s) 149, 224, 446, 838, 1667
BfmI CTRYAG 2 cut(s) 91, 2192
BfoI RGCGCY 1 cut(s) 288
BglII AGATCT 1 cut(s) 1933
Bme1390I CCNGG 9 cut(s) 118, 130, 160, 432, 457, 463, 464, 882, 1434
Bme18I GGWCC 8 cut(s) 428, 459, 1380, 1871, 1964, 2056, 2065, 2432
BmeT110I CYCGRG 3 cut(s) 353, 462, 2184
BmgBI CACGTC 1 cut(s) 304
BmgT120I GGNCC 9 cut(s) 428, 459, 1380, 1430, 1871, 1964, 2056, 2065, 2432
BmrFI CCNGG 9 cut(s) 118, 130, 160, 432, 457, 463, 464, 882, 1434
BmrI ACTGGG 1 cut(s) 2712
BmsI GCATC 4 cut(s) 901, 1318, 2003, 2539
BmtI GCTAGC 1 cut(s) 1896
BmuI ACTGGG 1 cut(s) 2712
BpiI GAAGAC 2 cut(s) 2108, 2759
BplI GAGNNNNNCTC 2 cut(s) 2623, 2655
Bpu10I CCTNAGC 3 cut(s) 512, 1875, 2640
BpuEI CTTGAG 5 cut(s) 65, 1405, 1914, 1953, 2237
BpuMI CCSGG 8 cut(s) 118, 130, 160, 432, 457, 463, 464, 882
Bsa29I ATCGAT 1 cut(s) 2341
BsaHI GRCGYC 2 cut(s) 285, 321
BsaI GGTCTC 3 cut(s) 149, 224, 446
BsaWI WCCGGW 3 cut(s) 177, 201, 856
BsaXI ACNNNNNCTCC 6 cut(s) 221, 251, 293, 323, 775, 805
Bse118I RCCGGY 3 cut(s) 215, 856, 1049
Bse1I ACTGG 5 cut(s) 719, 969, 2435, 2648, 2707
Bse3DI GCAATG 5 cut(s) 231, 412, 1254, 2550, 2892
BseBI CCWGG 1 cut(s) 1434
BseCI ATCGAT 1 cut(s) 2341
BseMI GCAATG 5 cut(s) 231, 412, 1254, 2550, 2892
BseNI ACTGG 5 cut(s) 719, 969, 2435, 2648, 2707
BseRI GAGGAG 3 cut(s) 2066, 2108, 2723
BseSI GKGCMC 1 cut(s) 2246
BseX3I CGGCCG 1 cut(s) 119
BseXI GCAGC 7 cut(s) 79, 105, 544, 1092, 2146, 2564, 2881
BseYI CCCAGC 1 cut(s) 63
BsgI GTGCAG 1 cut(s) 2145
Bsh1285I CGRYCG 4 cut(s) 122, 178, 206, 1702
BshFI GGCC 6 cut(s) 121, 545, 1243, 1431, 2099, 2851
BshNI GGYRCC 1 cut(s) 284
BshTI ACCGGT 1 cut(s) 856
BshVI ATCGAT 1 cut(s) 2341
BsiEI CGRYCG 4 cut(s) 122, 178, 206, 1702
BsiHKAI GWGCWC 1 cut(s) 281
BsiHKCI CYCGRG 3 cut(s) 353, 462, 2184
BsmAI GTCTC 5 cut(s) 149, 224, 446, 838, 1667
BsmI GAATGC 2 cut(s) 107, 2408
BsnI GGCC 6 cut(s) 121, 545, 1243, 1431, 2099, 2851
Bso31I GGTCTC 3 cut(s) 149, 224, 446
BsoBI CYCGRG 3 cut(s) 353, 462, 2184
Bsp1286I GDGCHC 2 cut(s) 281, 2246
Bsp19I CCATGG 2 cut(s) 2104, 2263
BspANI GGCC 6 cut(s) 121, 545, 1243, 1431, 2099, 2851
BspDI ATCGAT 1 cut(s) 2341
BspMAI CTGCAG 1 cut(s) 95
BspOI GCTAGC 1 cut(s) 1896
BspPI GGATC 7 cut(s) 170, 280, 2346, 2630, 2643, 2789, 2831
BspQI GCTCTTC 1 cut(s) 2576
BspT107I GGYRCC 1 cut(s) 284
BspTNI GGTCTC 3 cut(s) 149, 224, 446
BsrBI CCGCTC 2 cut(s) 295, 1635
BsrDI GCAATG 5 cut(s) 231, 412, 1254, 2550, 2892
BsrFI RCCGGY 3 cut(s) 215, 856, 1049
BsrI ACTGG 5 cut(s) 719, 969, 2435, 2648, 2707
BssAI RCCGGY 3 cut(s) 215, 856, 1049
BssNI GRCGYC 2 cut(s) 285, 321
BssSI CACGAG 2 cut(s) 30, 2576
BssT1I CCWWGG 5 cut(s) 959, 1231, 1464, 2104, 2263
Bst2BI CACGAG 2 cut(s) 30, 2576
Bst2UI CCWGG 1 cut(s) 1434
Bst6I CTCTTC 3 cut(s) 1888, 2522, 2576
BstACI GRCGYC 2 cut(s) 285, 321
BstAPI GCANNNNNTGC 3 cut(s) 538, 2549, 2891
BstC8I GCNNGC 5 cut(s) 543, 1646, 1894, 2349, 2883
BstDSI CCRYGG 2 cut(s) 2104, 2263
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 3 cut(s) 107, 287, 2730
BstMAI GTCTC 5 cut(s) 149, 224, 446, 838, 1667
BstMCI CGRYCG 4 cut(s) 122, 178, 206, 1702
BstMWI GCNNNNNNNGC 9 cut(s) 76, 102, 147, 538, 547, 1077, 1641, 2549, 2891
BstNI CCWGG 1 cut(s) 1434
BstNSI RCATGY 4 cut(s) 1680, 1795, 1821, 2048
BstSCI CCNGG 9 cut(s) 116, 128, 158, 430, 455, 461, 462, 880, 1432
BstSFI CTRYAG 2 cut(s) 91, 2192
BstSLI GKGCMC 1 cut(s) 2246
BstV1I GCAGC 7 cut(s) 79, 105, 544, 1092, 2146, 2564, 2881
BstV2I GAAGAC 2 cut(s) 2108, 2759
BstX2I RGATCY 2 cut(s) 1933, 2635
BstXI CCANNNNNNTGG 3 cut(s) 1100, 1864, 2155
BstYI RGATCY 2 cut(s) 1933, 2635
BstZI CGGCCG 1 cut(s) 119
Bsu15I ATCGAT 1 cut(s) 2341
BsuRI GGCC 6 cut(s) 121, 545, 1243, 1431, 2099, 2851
BsuTUI ATCGAT 1 cut(s) 2341
BtgI CCRYGG 2 cut(s) 2104, 2263
BtgZI GCGATG 1 cut(s) 516
BtrI CACGTC 1 cut(s) 304
BtsI GCAGTG 1 cut(s) 2044
BtsIMutI CAGTG 6 cut(s) 726, 1830, 1850, 2044, 2442, 2655
Cac8I GCNNGC 5 cut(s) 543, 1646, 1894, 2349, 2883
CaiI CAGNNNCTG 1 cut(s) 381
CfoI GCGC 3 cut(s) 107, 287, 2730
Cfr10I RCCGGY 3 cut(s) 215, 856, 1049
Cfr13I GGNCC 9 cut(s) 428, 459, 1380, 1430, 1871, 1964, 2056, 2065, 2432
Cfr9I CCCGGG 1 cut(s) 462
ClaI ATCGAT 1 cut(s) 2341
CpoI CGGWCCG 1 cut(s) 428
CseI GACGC 1 cut(s) 336
Csp6I GTAC 4 cut(s) 253, 842, 1612, 2464
CspAI ACCGGT 1 cut(s) 856
CspCI CAANNNNNGTGG 2 cut(s) 2385, 2420
CspI CGGWCCG 1 cut(s) 428
CviQI GTAC 4 cut(s) 253, 842, 1612, 2464
DinI GGCGCC 1 cut(s) 286
DrdI GACNNNNNNGTC 2 cut(s) 182, 320
DseDI GACNNNNNNGTC 2 cut(s) 182, 320
EaeI YGGCCR 2 cut(s) 119, 543
EagI CGGCCG 1 cut(s) 119
Eam1104I CTCTTC 3 cut(s) 1888, 2522, 2576
EarI CTCTTC 3 cut(s) 1888, 2522, 2576
EciI GGCGGA 1 cut(s) 361
Ecl136II GAGCTC 1 cut(s) 279
EclXI CGGCCG 1 cut(s) 119
Eco130I CCWWGG 5 cut(s) 959, 1231, 1464, 2104, 2263
Eco24I GRGCYC 1 cut(s) 281
Eco31I GGTCTC 3 cut(s) 149, 224, 446
Eco47I GGWCC 8 cut(s) 428, 459, 1380, 1871, 1964, 2056, 2065, 2432
Eco52I CGGCCG 1 cut(s) 119
Eco53kI GAGCTC 1 cut(s) 279
Eco57I CTGAAG 1 cut(s) 1746
Eco88I CYCGRG 3 cut(s) 353, 462, 2184
EcoICRI GAGCTC 1 cut(s) 279
EcoO109I RGGNCCY 4 cut(s) 1380, 1871, 1964, 2065
EcoRI GAATTC 2 cut(s) 694, 1357
EcoRII CCWGG 1 cut(s) 1432
EcoT14I CCWWGG 5 cut(s) 959, 1231, 1464, 2104, 2263
EcoT22I ATGCAT 1 cut(s) 1333
EcoT38I GRGCYC 1 cut(s) 281
EgeI GGCGCC 1 cut(s) 286
EheI GGCGCC 1 cut(s) 286
ErhI CCWWGG 5 cut(s) 959, 1231, 1464, 2104, 2263
FalI AAGNNNNNCTT 2 cut(s) 2751, 2783
FauI CCCGC 2 cut(s) 300, 907
FauNDI CATATG 2 cut(s) 1248, 2248
FbaI TGATCA 3 cut(s) 769, 823, 1510
FblI GTMKAC 2 cut(s) 174, 306
FriOI GRGCYC 1 cut(s) 281
GlaI GCGC 3 cut(s) 106, 286, 2729
GsaI CCCAGC 1 cut(s) 67
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 6 cut(s) 121, 545, 1243, 1431, 2099, 2851
HgaI GACGC 1 cut(s) 336
HhaI GCGC 3 cut(s) 107, 287, 2730
Hin1I GRCGYC 2 cut(s) 285, 321
Hin6I GCGC 3 cut(s) 105, 285, 2728
HinP1I GCGC 3 cut(s) 105, 285, 2728
HincII GTYRAC 4 cut(s) 175, 307, 2384, 2700
HindII GTYRAC 4 cut(s) 175, 307, 2384, 2700
HindIII AAGCTT 2 cut(s) 774, 2083
HinfI GANTC 9 cut(s) 308, 378, 423, 506, 1016, 1036, 1459, 1799, 2843
Hpy166II GTNNAC 9 cut(s) 175, 301, 307, 940, 1497, 1696, 2260, 2384, 2700
Hpy8I GTNNAC 9 cut(s) 175, 301, 307, 940, 1497, 1696, 2260, 2384, 2700
Hpy99I CGWCG 7 cut(s) 176, 188, 308, 317, 320, 323, 330
HpyCH4IV ACGT 7 cut(s) 183, 303, 321, 625, 705, 936, 2400
HpyF10VI GCNNNNNNNGC 9 cut(s) 76, 102, 147, 538, 547, 1077, 1641, 2549, 2891
HpySE526I ACGT 7 cut(s) 183, 303, 321, 625, 705, 936, 2400
Hsp92I GRCGYC 2 cut(s) 285, 321
HspAI GCGC 3 cut(s) 105, 285, 2728
KasI GGCGCC 1 cut(s) 284
Ksp22I TGATCA 3 cut(s) 769, 823, 1510
LguI GCTCTTC 1 cut(s) 2576
LmnI GCTCC 7 cut(s) 141, 204, 264, 284, 965, 2483, 2710
Lsp1109I GCAGC 7 cut(s) 79, 105, 544, 1092, 2146, 2564, 2881
LweI GCATC 4 cut(s) 901, 1318, 2003, 2539
MaeII ACGT 7 cut(s) 183, 303, 321, 625, 705, 936, 2400
MbiI CCGCTC 2 cut(s) 295, 1635
MfeI CAATTG 1 cut(s) 536
MflI RGATCY 2 cut(s) 1933, 2635
MhlI GDGCHC 2 cut(s) 281, 2246
MlsI TGGCCA 1 cut(s) 545
MluNI TGGCCA 1 cut(s) 545
Mly113I GGCGCC 1 cut(s) 285
MlyI GAGTC 3 cut(s) 302, 372, 432
MmeI TCCRAC 7 cut(s) 336, 349, 406, 1420, 2083, 2172, 2822
Mox20I TGGCCA 1 cut(s) 545
Mph1103I ATGCAT 1 cut(s) 1333
MroXI GAANNNNTTC 4 cut(s) 1226, 1620, 2115, 2571
MscI TGGCCA 1 cut(s) 545
MseI TTAA 6 cut(s) 489, 903, 1073, 1419, 2081, 2483
MslI CAYNNNNRTG 4 cut(s) 501, 1008, 1452, 1454
Msp20I TGGCCA 1 cut(s) 545
MspA1I CMGCKG 3 cut(s) 1024, 1126, 1169
MspR9I CCNGG 9 cut(s) 118, 130, 160, 432, 457, 463, 464, 882, 1434
MunI CAATTG 1 cut(s) 536
Mva1269I GAATGC 2 cut(s) 107, 2408
MvaI CCWGG 1 cut(s) 1434
MwoI GCNNNNNNNGC 9 cut(s) 76, 102, 147, 538, 547, 1077, 1641, 2549, 2891
NarI GGCGCC 1 cut(s) 285
NciI CCSGG 8 cut(s) 118, 130, 160, 432, 457, 463, 464, 882
NcoI CCATGG 2 cut(s) 2104, 2263
NdeI CATATG 2 cut(s) 1248, 2248
NheI GCTAGC 1 cut(s) 1892
NmeAIII GCCGAG 1 cut(s) 815
NmuCI GTSAC 4 cut(s) 1903, 2422, 2437, 2455
NsiI ATGCAT 1 cut(s) 1333
NspI RCATGY 4 cut(s) 1680, 1795, 1821, 2048
OliI CACNNNNGTG 1 cut(s) 1452
PaeR7I CTCGAG 2 cut(s) 353, 2184
PciI ACATGT 1 cut(s) 1817
PciSI GCTCTTC 1 cut(s) 2576
PcsI WCGNNNNNNNCGW 5 cut(s) 180, 303, 309, 312, 351
PctI GAATGC 2 cut(s) 107, 2408
PdmI GAANNNNTTC 4 cut(s) 1226, 1620, 2115, 2571
PfeI GAWTC 6 cut(s) 506, 1016, 1036, 1459, 1799, 2843
PflFI GACNNNGTC 2 cut(s) 320, 432
PflMI CCANNNNNTGG 2 cut(s) 381, 1299
PfoI TCCNGGA 1 cut(s) 128
PinAI ACCGGT 1 cut(s) 856
PleI GAGTC 3 cut(s) 302, 372, 431
PluTI GGCGCC 1 cut(s) 288
PpsI GAGTC 3 cut(s) 302, 372, 431
PpuMI RGGWCCY 4 cut(s) 1380, 1871, 1964, 2065
PscI ACATGT 1 cut(s) 1817
PsiI TTATAA 1 cut(s) 2225
Psp124BI GAGCTC 1 cut(s) 281
Psp1406I AACGTT 1 cut(s) 2400
Psp5II RGGWCCY 4 cut(s) 1380, 1871, 1964, 2065
Psp6I CCWGG 1 cut(s) 1432
PspFI CCCAGC 1 cut(s) 63
PspGI CCWGG 1 cut(s) 1432
PspPI GGNCC 9 cut(s) 428, 459, 1380, 1430, 1871, 1964, 2056, 2065, 2432
PspPPI RGGWCCY 4 cut(s) 1380, 1871, 1964, 2065
PstI CTGCAG 1 cut(s) 95
PstNI CAGNNNCTG 1 cut(s) 381
PsuI RGATCY 2 cut(s) 1933, 2635
PsyI GACNNNGTC 2 cut(s) 320, 432
PvuII CAGCTG 2 cut(s) 1024, 1169
RsaI GTAC 4 cut(s) 254, 843, 1613, 2465
RsaNI GTAC 4 cut(s) 253, 842, 1612, 2464
RseI CAYNNNNRTG 4 cut(s) 501, 1008, 1452, 1454
Rsr2I CGGWCCG 1 cut(s) 428
RsrII CGGWCCG 1 cut(s) 428
SacI GAGCTC 1 cut(s) 281
SalI GTCGAC 2 cut(s) 173, 305
SapI GCTCTTC 1 cut(s) 2576
SaqAI TTAA 6 cut(s) 489, 903, 1073, 1419, 2081, 2483
Sau96I GGNCC 9 cut(s) 428, 459, 1380, 1430, 1871, 1964, 2056, 2065, 2432
SchI GAGTC 3 cut(s) 302, 372, 432
ScrFI CCNGG 9 cut(s) 118, 130, 160, 432, 457, 463, 464, 882, 1434
SduI GDGCHC 2 cut(s) 281, 2246
SfaNI GCATC 4 cut(s) 901, 1318, 2003, 2539
SfcI CTRYAG 2 cut(s) 91, 2192
SfoI GGCGCC 1 cut(s) 286
Sfr274I CTCGAG 2 cut(s) 353, 2184
SinI GGWCC 8 cut(s) 428, 459, 1380, 1871, 1964, 2056, 2065, 2432
SlaI CTCGAG 2 cut(s) 353, 2184
SmaI CCCGGG 1 cut(s) 464
SmiMI CAYNNNNRTG 4 cut(s) 501, 1008, 1452, 1454
SmlI CTYRAG 7 cut(s) 80, 353, 1384, 1929, 1968, 2184, 2216
SmoI CTYRAG 7 cut(s) 80, 353, 1384, 1929, 1968, 2184, 2216
SspDI GGCGCC 1 cut(s) 284
SspI AATATT 2 cut(s) 1472, 1831
SstI GAGCTC 1 cut(s) 281
StyD4I CCNGG 9 cut(s) 116, 128, 158, 430, 455, 461, 462, 880, 1432
StyI CCWWGG 5 cut(s) 959, 1231, 1464, 2104, 2263
TaiI ACGT 7 cut(s) 186, 306, 324, 628, 708, 939, 2403
TaqI TCGA 9 cut(s) 174, 206, 275, 306, 345, 354, 1214, 2185, 2341
TatI WGTACW 2 cut(s) 841, 2463
TauI GCSGC 6 cut(s) 124, 153, 290, 485, 1638, 2854
TfiI GAWTC 6 cut(s) 506, 1016, 1036, 1459, 1799, 2843
Tru1I TTAA 6 cut(s) 489, 903, 1073, 1419, 2081, 2483
Tru9I TTAA 6 cut(s) 489, 903, 1073, 1419, 2081, 2483
TscAI CASTG 6 cut(s) 726, 1830, 1857, 2044, 2442, 2655
TseFI GTSAC 4 cut(s) 1903, 2422, 2437, 2455
TseI GCWGC 7 cut(s) 67, 93, 532, 1080, 2159, 2552, 2894
Tsp45I GTSAC 4 cut(s) 1903, 2422, 2437, 2455
TspGWI ACGGA 3 cut(s) 160, 435, 1029
TspMI CCCGGG 1 cut(s) 462
TspRI CASTG 6 cut(s) 726, 1830, 1857, 2044, 2442, 2655
Tth111I GACNNNGTC 2 cut(s) 320, 432
Van91I CCANNNNNTGG 2 cut(s) 381, 1299
VpaK11BI GGWCC 8 cut(s) 428, 459, 1380, 1871, 1964, 2056, 2065, 2432
XapI RAATTY 4 cut(s) 694, 757, 1357, 2443
XceI RCATGY 4 cut(s) 1680, 1795, 1821, 2048
XhoI CTCGAG 2 cut(s) 353, 2184
XmaI CCCGGG 1 cut(s) 462
XmiI GTMKAC 2 cut(s) 174, 306
XmnI GAANNNNTTC 4 cut(s) 1226, 1620, 2115, 2571
ZraI GACGTC 1 cut(s) 322
Zsp2I ATGCAT 1 cut(s) 1333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.