MD15G1176700.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013582
Physical Location & Seq
Forward (+)
13833024 .. 13835723
2700 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1176700.v1.1.491

Sequence Viewer

Length: 2700 bp
ATGACAACCATGAAAGAGCCACCTCTCTCCCGTCGCAGAAGGCCCACTTGCCACCAGCCCAAGAAGCTGAAGCTCGTCTGCTGCTTCAACGGCGCATTCCACCCCCGCCCGCCGTCCGGGAAGCTACGCTACGTCGGCGGCGAGGCCAGAATCGTGTCCGTGGACCGTAACATCGGGTTCTCGAAGCTCCGATCAAAGATTTTGGATCTTTGCCCCAGCATTAACCCCACATTCACTCTCAAGTACCAGCTCCCGGGTTCCGGCTCCGGCTCCGACTCCGCTGCCCCTCTCGCCCTCATCGCGTCAGATGACGACGTTCGGTGCATGATCGAAGAGTACGACAAGCTCGAGCTGTACGGTAAGTCCCCGAGGCTCTGGGTTTTTGTTTGTTGTAGAAATGGCAGTGATAATGTCAGTGGTTATGTGAATTGCGTGAAAGGTGTGAACCTTAGTGAGGGGTTTGAGTCAGAGAGCGAACATGTAGGGACCCACCGCCGCCATTTGAGGGGCGGAGCTCAGGTGGAGAAGAAGGCGGCGGTGAAGAACTTTGGGGGCGTACGGTCCGGCGATGAATCGCTTAGAAAAATGGTGCTGAAACGGCAATTGCTTGCAAAGCAATCGGCTTTTCGCCGTCGTTTTGGTACTAGTGAGAATGATGAAATAGGGTTTTTGGGTGAATCCCAAAAGTGTGAATCTTTTGATCAGGGCAGAGACTTGTTGGATGACAGATTAGGGAGTAAACGTTGTGACAATGTAGGGAGTTTTTTGGTGTCTCGGGCAAATCCGCTGAATCCCAGAGATGGGAATTTGAGGGTGGAAGGTAAGGGTTTTGGGCTGCGCTGTCCGGCATGGTCGGGTCAGATTTTGTGTAATGGGGTTGGGAGGTCGAGTCAGGCAGTGGCAACTGCGCCGCCTGCGAAACCATTACCGGGTTGTAATGGTTGTTGTAGTGATGTTAAGCAGGATTTTGAAAACAATGCAATGGCTGGGATTAGTTATGTGAACAGAGAAAACGTTATGCCTTTGGAGACCAATTGTGATTTGGTGAAGAATGGATTGAGTTGTAATTCTGGTGCTGATTCTTTGGCTGGAACTACTGCGTATCCTGTGAAGTCTTCTTATAGTGGTGATAGGGTGTGGGGCGGCTTAAAGAGCGGGATTCGTAACCATAGATTTGGTGTGAATGATGCTAGAAATCAGCGGTGTTATCAGTACCATGTTCGGAATCATCATAGGAGCAACATGACTGAGATGGGGATCCAAAGAACGAAGCAGGGGATTTCAGCTTGGAAATGCTACCCCGGACTTATGCCAAATTTGAACATTGCAAAGCAAGGGCAGTCTCTGAGAGTATATCATCCGAGTATATGGAGGCAATGCTCTGGTTTTCCTGAGCATGCAGTGGAAGAAAGGGTGAGGATGATGGATTCTCGTTCAATGAAAGACAACTACTTGATCGATGTGCTATATGGTAATGAGAAACTGAGTAAACGGAGTGGCCCTGCAGTTTCACAGCAAGGGAATTATAATCCGAGTAAATCCTATTCAGGTTTTTGTGGTGCATTTACTGGCGTGGGAAATCAATCTCTCATGAGTTCTAATGCCATTGAGAGTTCATCTCCATCTGTGAAGACAATGGACATTCTGGAAGATCTCTTAACTGGTTCTGATTTTGGGAAGTGTGATGGTCCATATCAAACTTCACATGAAAATTTTCATGGAATGTCCGGAAATTGTGAGTTTCAGAAGGAGATGCGCCTGTTGGACTCTCATGAGGTTGCTAACACCTCTGGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTGTTTTTTTTTTTATATTGTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

900

Amino Acids

109.79

Weight (kDa)

9.09

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 39 - 128 1.1e-10 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013582)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03810
malus_domestica MD02G1036600.v1.1 MD15G1176700.v1.1
prunus_persica Prupe.7G238800_v2.0.a1
pyrus_communis pycom02g03010 pycom15g15820
rosa_chinensis RchiOBHm_Chr2g0089211
rosa_laevigata RLG00000016005
rosa_multiflora Rmu_sc0008322.1_g000024 Rmu_sc0030891.1_g000001
rosa_roxburghii Rroxscaffold_2G00152140
rosa_rugosa Rorug01G0488400 Rorug01G0488500
rosa_samantha Rh2AG044100 Rh2BG042800 Rh2CG044300 Rh2DG043100
rosa_wichuraiana Rw2G003900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1527
AccBSI CCGCTC 1 cut(s) 1155
AccII CGCG 1 cut(s) 302
AccIII TCCGGA 1 cut(s) 1727
AclI AACGTT 2 cut(s) 742, 1014
AclWI GGATC 3 cut(s) 213, 1252, 1265
AcsI RAATTY 3 cut(s) 805, 1315, 1711
AcuI CTGAAG 1 cut(s) 89
AfaI GTAC 6 cut(s) 245, 338, 356, 558, 643, 1214
AfiI CCNNNNNNNGG 8 cut(s) 116, 253, 260, 454, 505, 800, 801, 929
AflIII ACRYGT 1 cut(s) 478
AgsI TTSAA 4 cut(s) 88, 971, 1321, 1437
AhlI ACTAGT 1 cut(s) 644
AluBI AGCT 9 cut(s) 67, 73, 124, 187, 250, 346, 352, 515, 1286
AluI AGCT 9 cut(s) 67, 73, 124, 187, 250, 346, 352, 515, 1286
Alw21I GWGCWC 1 cut(s) 517
Alw26I GTCTC 4 cut(s) 705, 777, 1022, 1347
AlwI GGATC 3 cut(s) 213, 1252, 1265
AlwNI CAGNNNCTG 1 cut(s) 1345
Ama87I CYCGRG 4 cut(s) 253, 347, 367, 774
Aor13HI TCCGGA 1 cut(s) 1727
AoxI GGCC 3 cut(s) 41, 144, 1498
ApeKI GCWGC 3 cut(s) 81, 281, 835
ApoI RAATTY 3 cut(s) 805, 1315, 1711
Asp700I GAANNNNTTC 1 cut(s) 1713
AspLEI GCGC 4 cut(s) 95, 840, 910, 1758
AspS9I GGNCC 6 cut(s) 42, 163, 486, 561, 1499, 1688
AsuC2I CCSGG 5 cut(s) 118, 254, 255, 930, 1302
AsuHPI GGTGA 5 cut(s) 550, 686, 1057, 1139, 1426
AvaI CYCGRG 4 cut(s) 253, 347, 367, 774
AvaII GGWCC 4 cut(s) 163, 486, 561, 1688
BamHI GGATCC 1 cut(s) 1257
BanII GRGCYC 1 cut(s) 517
BarI GAAGNNNNNNTAC 2 cut(s) 113, 145
BbsI GAAGAC 2 cut(s) 1107, 1637
Bbv12I GWGCWC 1 cut(s) 517
BbvI GCAGC 3 cut(s) 68, 268, 822
BccI CCATC 5 cut(s) 794, 1246, 1417, 1630, 1679
BceAI ACGGC 4 cut(s) 97, 106, 614, 615
BcgI CGANNNNNNTGC 4 cut(s) 263, 297, 600, 634
BciVI GTATCC 1 cut(s) 1113
BclI TGATCA 1 cut(s) 700
BcnI CCSGG 5 cut(s) 118, 254, 255, 930, 1302
BcoDI GTCTC 4 cut(s) 705, 777, 1022, 1347
BcuI ACTAGT 1 cut(s) 644
BfaI CTAG 2 cut(s) 645, 1191
BfmI CTRYAG 1 cut(s) 1503
BfuI GTATCC 1 cut(s) 1113
BglII AGATCT 1 cut(s) 1651
BisI GCNGC 8 cut(s) 82, 139, 282, 496, 534, 836, 911, 1144
BlsI GCNGC 8 cut(s) 83, 140, 283, 497, 535, 837, 912, 1145
Bme1390I CCNGG 5 cut(s) 118, 254, 255, 930, 1302
Bme18I GGWCC 4 cut(s) 163, 486, 561, 1688
BmeT110I CYCGRG 4 cut(s) 253, 347, 367, 774
BmgT120I GGNCC 6 cut(s) 42, 163, 486, 561, 1499, 1688
BmiI GGNNCC 6 cut(s) 259, 265, 271, 487, 488, 1259
BmrFI CCNGG 5 cut(s) 118, 254, 255, 930, 1302
BmsI GCATC 2 cut(s) 1177, 1743
BpiI GAAGAC 2 cut(s) 1107, 1637
BplI GAGNNNNNCTC 2 cut(s) 1603, 1635
Bpu10I CCTNAGC 2 cut(s) 516, 1392
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 5 cut(s) 118, 254, 255, 930, 1302
Bsa29I ATCGAT 1 cut(s) 1458
BsaBI GATNNNNATC 1 cut(s) 1256
BsaI GGTCTC 1 cut(s) 1022
BsaJI CCNNGG 4 cut(s) 159, 253, 368, 1300
BsaWI WCCGGW 1 cut(s) 1727
BsaXI ACNNNNNCTCC 6 cut(s) 504, 534, 727, 757, 874, 904
Bsc4I CCNNNNNNNGG 8 cut(s) 116, 253, 260, 454, 505, 800, 801, 929
Bse1I ACTGG 2 cut(s) 1573, 1666
Bse3DI GCAATG 3 cut(s) 987, 1323, 1382
Bse8I GATNNNNATC 1 cut(s) 1256
BseAI TCCGGA 1 cut(s) 1727
BseCI ATCGAT 1 cut(s) 1458
BseDI CCNNGG 4 cut(s) 159, 253, 368, 1300
BseGI GGATG 3 cut(s) 727, 1357, 1425
BseJI GATNNNNATC 1 cut(s) 1256
BseLI CCNNNNNNNGG 8 cut(s) 116, 253, 260, 454, 505, 800, 801, 929
BseMI GCAATG 3 cut(s) 987, 1323, 1382
BseMII CTCAG 5 cut(s) 530, 1239, 1337, 1383, 1475
BseNI ACTGG 2 cut(s) 1573, 1666
BseXI GCAGC 3 cut(s) 68, 268, 822
BseYI CCCAGC 2 cut(s) 215, 986
Bsh1236I CGCG 1 cut(s) 302
BshFI GGCC 3 cut(s) 43, 146, 1500
BshVI ATCGAT 1 cut(s) 1458
BsiHKAI GWGCWC 1 cut(s) 517
BsiHKCI CYCGRG 4 cut(s) 253, 347, 367, 774
BsiSI CCGG 9 cut(s) 117, 254, 261, 267, 564, 845, 929, 1302, 1728
BsiWI CGTACG 1 cut(s) 556
BslFI GGGAC 2 cut(s) 349, 499
BslI CCNNNNNNNGG 8 cut(s) 116, 253, 260, 454, 505, 800, 801, 929
BsmAI GTCTC 4 cut(s) 705, 777, 1022, 1347
BsmFI GGGAC 2 cut(s) 349, 499
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 3 cut(s) 43, 146, 1500
Bso31I GGTCTC 1 cut(s) 1022
BsoBI CYCGRG 4 cut(s) 253, 347, 367, 774
Bsp1286I GDGCHC 1 cut(s) 517
Bsp13I TCCGGA 1 cut(s) 1727
Bsp143I GATC 7 cut(s) 191, 205, 327, 700, 1257, 1455, 1651
BspANI GGCC 3 cut(s) 43, 146, 1500
BspCNI CTCAG 5 cut(s) 529, 1240, 1338, 1384, 1476
BspDI ATCGAT 1 cut(s) 1458
BspEI TCCGGA 1 cut(s) 1727
BspFNI CGCG 1 cut(s) 302
BspHI TCATGA 2 cut(s) 1590, 1771
BspLI GGNNCC 6 cut(s) 259, 265, 271, 487, 488, 1259
BspMAI CTGCAG 1 cut(s) 1507
BspPI GGATC 3 cut(s) 213, 1252, 1265
BspTNI GGTCTC 1 cut(s) 1022
BsrBI CCGCTC 1 cut(s) 1155
BsrDI GCAATG 3 cut(s) 987, 1323, 1382
BsrI ACTGG 2 cut(s) 1573, 1666
BssECI CCNNGG 4 cut(s) 159, 253, 368, 1300
BssMI GATC 7 cut(s) 191, 205, 327, 700, 1257, 1455, 1651
Bst4CI ACNGT 3 cut(s) 167, 359, 561
Bst6I CTCTTC 1 cut(s) 327
BstC8I GCNNGC 4 cut(s) 110, 609, 915, 1398
BstDEI CTNAG 7 cut(s) 449, 516, 578, 1248, 1346, 1392, 1484
BstDSI CCRYGG 1 cut(s) 159
BstENI CCTNNNNNAGG 1 cut(s) 452
BstF5I GGATG 3 cut(s) 727, 1357, 1425
BstFNI CGCG 1 cut(s) 302
BstHHI GCGC 4 cut(s) 95, 840, 910, 1758
BstKTI GATC 7 cut(s) 194, 208, 330, 703, 1260, 1458, 1654
BstMAI GTCTC 4 cut(s) 705, 777, 1022, 1347
BstMBI GATC 7 cut(s) 191, 205, 327, 700, 1257, 1455, 1651
BstMWI GCNNNNNNNGC 9 cut(s) 64, 90, 135, 290, 299, 598, 613, 914, 1152
BstNSI RCATGY 2 cut(s) 482, 1400
BstSCI CCNGG 5 cut(s) 116, 252, 253, 928, 1300
BstSFI CTRYAG 1 cut(s) 1503
BstUI CGCG 1 cut(s) 302
BstV1I GCAGC 3 cut(s) 68, 268, 822
BstV2I GAAGAC 2 cut(s) 1107, 1637
BstX2I RGATCY 3 cut(s) 205, 1257, 1651
BstXI CCANNNNNNTGG 1 cut(s) 1175
BstYI RGATCY 3 cut(s) 205, 1257, 1651
Bsu15I ATCGAT 1 cut(s) 1458
BsuI GTATCC 1 cut(s) 1113
BsuRI GGCC 3 cut(s) 43, 146, 1500
BsuTUI ATCGAT 1 cut(s) 1458
BtgI CCRYGG 1 cut(s) 159
BtgZI GCGATG 2 cut(s) 283, 582
BtsCI GGATG 3 cut(s) 727, 1357, 1425
BtsI GCAGTG 3 cut(s) 409, 903, 1407
BtsIMutI CAGTG 4 cut(s) 409, 421, 903, 1407
Cac8I GCNNGC 4 cut(s) 110, 609, 915, 1398
CaiI CAGNNNCTG 1 cut(s) 1345
CciI TCATGA 2 cut(s) 1590, 1771
CfoI GCGC 4 cut(s) 95, 840, 910, 1758
Cfr13I GGNCC 6 cut(s) 42, 163, 486, 561, 1499, 1688
Cfr9I CCCGGG 1 cut(s) 253
ClaI ATCGAT 1 cut(s) 1458
CpoI CGGWCCG 1 cut(s) 561
CseI GACGC 1 cut(s) 291
Csp6I GTAC 6 cut(s) 244, 337, 355, 557, 642, 1213
CspI CGGWCCG 1 cut(s) 561
CviQI GTAC 6 cut(s) 244, 337, 355, 557, 642, 1213
DdeI CTNAG 7 cut(s) 449, 516, 578, 1248, 1346, 1392, 1484
DpnI GATC 7 cut(s) 193, 207, 329, 702, 1259, 1457, 1653
DpnII GATC 7 cut(s) 191, 205, 327, 700, 1257, 1455, 1651
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
EciI GGCGGA 1 cut(s) 525
Ecl136II GAGCTC 1 cut(s) 515
Eco24I GRGCYC 1 cut(s) 517
Eco31I GGTCTC 1 cut(s) 1022
Eco47I GGWCC 4 cut(s) 163, 486, 561, 1688
Eco53kI GAGCTC 1 cut(s) 515
Eco57I CTGAAG 1 cut(s) 89
Eco88I CYCGRG 4 cut(s) 253, 347, 367, 774
EcoICRI GAGCTC 1 cut(s) 515
EcoNI CCTNNNNNAGG 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 486
EcoT38I GRGCYC 1 cut(s) 517
FalI AAGNNNNNCTT 2 cut(s) 31, 63
FaqI GGGAC 2 cut(s) 349, 499
FauI CCCGC 3 cut(s) 113, 117, 1148
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 8 cut(s) 82, 139, 282, 496, 534, 836, 911, 1144
FokI GGATG 3 cut(s) 734, 1344, 1432
FriOI GRGCYC 1 cut(s) 517
Fsp4HI GCNGC 8 cut(s) 82, 139, 282, 496, 534, 836, 911, 1144
FspBI CTAG 2 cut(s) 645, 1191
GlaI GCGC 4 cut(s) 94, 839, 909, 1757
GluI GCNGC 8 cut(s) 82, 139, 282, 496, 534, 836, 911, 1144
GsaI CCCAGC 2 cut(s) 219, 990
HaeIII GGCC 3 cut(s) 43, 146, 1500
HapII CCGG 9 cut(s) 117, 254, 261, 267, 564, 845, 929, 1302, 1728
HgaI GACGC 1 cut(s) 291
HhaI GCGC 4 cut(s) 95, 840, 910, 1758
Hin6I GCGC 4 cut(s) 93, 838, 908, 1756
HinP1I GCGC 4 cut(s) 93, 838, 908, 1756
HpaII CCGG 9 cut(s) 117, 254, 261, 267, 564, 845, 929, 1302, 1728
HphI GGTGA 5 cut(s) 550, 686, 1057, 1139, 1426
Hpy166II GTNNAC 5 cut(s) 163, 445, 740, 1003, 1490
Hpy188III TCNNGA 6 cut(s) 181, 1391, 1591, 1646, 1728, 1772
Hpy8I GTNNAC 5 cut(s) 163, 445, 740, 1003, 1490
Hpy99I CGWCG 4 cut(s) 36, 137, 317, 636
HpyAV CCTTC 4 cut(s) 33, 523, 812, 1741
HpyCH4III ACNGT 3 cut(s) 167, 359, 561
HpyCH4IV ACGT 4 cut(s) 132, 315, 742, 1014
HpyCH4V TGCA 7 cut(s) 324, 611, 980, 1328, 1400, 1505, 1562
HpyF10VI GCNNNNNNNGC 9 cut(s) 64, 90, 135, 290, 299, 598, 613, 914, 1152
HpyF3I CTNAG 7 cut(s) 449, 516, 578, 1248, 1346, 1392, 1484
HpySE526I ACGT 4 cut(s) 132, 315, 742, 1014
HspAI GCGC 4 cut(s) 93, 838, 908, 1756
KflI GGGWCCC 1 cut(s) 486
Kpn2I TCCGGA 1 cut(s) 1727
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 7 cut(s) 191, 205, 327, 700, 1257, 1455, 1651
LmnI GCTCC 6 cut(s) 192, 255, 269, 275, 512, 1236
Lsp1109I GCAGC 3 cut(s) 68, 268, 822
LweI GCATC 2 cut(s) 1177, 1743
MaeI CTAG 2 cut(s) 645, 1191
MaeII ACGT 4 cut(s) 132, 315, 742, 1014
MaeIII GTNAC 3 cut(s) 167, 746, 1163
MalI GATC 7 cut(s) 193, 207, 329, 702, 1259, 1457, 1653
MbiI CCGCTC 1 cut(s) 1155
MboI GATC 7 cut(s) 191, 205, 327, 700, 1257, 1455, 1651
MboII GAAGA 8 cut(s) 344, 538, 553, 1060, 1107, 1418, 1642, 1661
MfeI CAATTG 2 cut(s) 602, 1033
MflI RGATCY 3 cut(s) 205, 1257, 1651
MhlI GDGCHC 1 cut(s) 517
MluCI AATT 9 cut(s) 427, 602, 805, 1033, 1066, 1315, 1522, 1711, 1732
MlyI GAGTC 4 cut(s) 269, 473, 898, 1760
MmeI TCCRAC 3 cut(s) 297, 699, 1743
MroI TCCGGA 1 cut(s) 1727
MroXI GAANNNNTTC 1 cut(s) 1713
MseI TTAA 5 cut(s) 222, 957, 1148, 1658, 2698
MspA1I CMGCKG 3 cut(s) 281, 787, 1201
MspI CCGG 9 cut(s) 117, 254, 261, 267, 564, 845, 929, 1302, 1728
MspR9I CCNGG 5 cut(s) 118, 254, 255, 930, 1302
MunI CAATTG 2 cut(s) 602, 1033
Mva1269I GAATGC 1 cut(s) 95
MvnI CGCG 1 cut(s) 302
MwoI GCNNNNNNNGC 9 cut(s) 64, 90, 135, 290, 299, 598, 613, 914, 1152
NciI CCSGG 5 cut(s) 118, 254, 255, 930, 1302
NdeII GATC 7 cut(s) 191, 205, 327, 700, 1257, 1455, 1651
NlaIV GGNNCC 6 cut(s) 259, 265, 271, 487, 488, 1259
NmuCI GTSAC 1 cut(s) 746
NspI RCATGY 2 cut(s) 482, 1400
PaeI GCATGC 1 cut(s) 1400
PaeR7I CTCGAG 1 cut(s) 347
PagI TCATGA 2 cut(s) 1590, 1771
PciI ACATGT 1 cut(s) 478
PcsI WCGNNNNNNNCGW 3 cut(s) 138, 336, 345
PctI GAATGC 1 cut(s) 95
PdmI GAANNNNTTC 1 cut(s) 1713
PfeI GAWTC 9 cut(s) 150, 572, 677, 692, 790, 1079, 1159, 1225, 1427
Pfl23II CGTACG 1 cut(s) 556
PfoI TCCNGGA 1 cut(s) 116
PkrI GCNGC 8 cut(s) 83, 140, 283, 497, 535, 837, 912, 1145
PleI GAGTC 4 cut(s) 269, 472, 897, 1760
PpsI GAGTC 4 cut(s) 269, 472, 897, 1760
PpuMI RGGWCCY 1 cut(s) 486
PscI ACATGT 1 cut(s) 478
PsiI TTATAA 1 cut(s) 1527
Psp124BI GAGCTC 1 cut(s) 517
Psp1406I AACGTT 2 cut(s) 742, 1014
Psp5II RGGWCCY 1 cut(s) 486
PspFI CCCAGC 2 cut(s) 215, 986
PspLI CGTACG 1 cut(s) 556
PspN4I GGNNCC 6 cut(s) 259, 265, 271, 487, 488, 1259
PspPI GGNCC 6 cut(s) 42, 163, 486, 561, 1499, 1688
PspPPI RGGWCCY 1 cut(s) 486
PspXI VCTCGAGB 1 cut(s) 347
PstI CTGCAG 1 cut(s) 1507
PstNI CAGNNNCTG 1 cut(s) 1345
PsuI RGATCY 3 cut(s) 205, 1257, 1651
RsaI GTAC 6 cut(s) 245, 338, 356, 558, 643, 1214
RsaNI GTAC 6 cut(s) 244, 337, 355, 557, 642, 1213
Rsr2I CGGWCCG 1 cut(s) 561
RsrII CGGWCCG 1 cut(s) 561
SacI GAGCTC 1 cut(s) 517
SaqAI TTAA 5 cut(s) 222, 957, 1148, 1658, 2698
SatI GCNGC 8 cut(s) 82, 139, 282, 496, 534, 836, 911, 1144
Sau3AI GATC 7 cut(s) 191, 205, 327, 700, 1257, 1455, 1651
Sau96I GGNCC 6 cut(s) 42, 163, 486, 561, 1499, 1688
SchI GAGTC 4 cut(s) 269, 473, 898, 1760
ScrFI CCNGG 5 cut(s) 118, 254, 255, 930, 1302
SduI GDGCHC 1 cut(s) 517
SfaNI GCATC 2 cut(s) 1177, 1743
SfcI CTRYAG 1 cut(s) 1503
Sfr274I CTCGAG 1 cut(s) 347
SinI GGWCC 4 cut(s) 163, 486, 561, 1688
SlaI CTCGAG 1 cut(s) 347
SmaI CCCGGG 1 cut(s) 255
SmlI CTYRAG 2 cut(s) 239, 347
SmoI CTYRAG 2 cut(s) 239, 347
SpeI ACTAGT 1 cut(s) 644
SphI GCATGC 1 cut(s) 1400
Sse9I AATT 9 cut(s) 427, 602, 805, 1033, 1066, 1315, 1522, 1711, 1732
SspMI CTAG 2 cut(s) 645, 1191
SstI GAGCTC 1 cut(s) 517
StyD4I CCNGG 5 cut(s) 116, 252, 253, 928, 1300
TaaI ACNGT 3 cut(s) 167, 359, 561
TaiI ACGT 4 cut(s) 135, 318, 745, 1017
TaqI TCGA 5 cut(s) 182, 330, 348, 887, 1458
TasI AATT 9 cut(s) 427, 602, 805, 1033, 1066, 1315, 1522, 1711, 1732
TauI GCSGC 5 cut(s) 141, 498, 536, 913, 1146
TfiI GAWTC 9 cut(s) 150, 572, 677, 692, 790, 1079, 1159, 1225, 1427
Tru1I TTAA 5 cut(s) 222, 957, 1148, 1658, 2698
Tru9I TTAA 5 cut(s) 222, 957, 1148, 1658, 2698
TscAI CASTG 4 cut(s) 409, 421, 903, 1407
TseFI GTSAC 1 cut(s) 746
TseI GCWGC 3 cut(s) 81, 281, 835
Tsp45I GTSAC 1 cut(s) 746
TspDTI ATGAA 7 cut(s) 26, 585, 672, 1454, 1605, 1706, 1722
TspGWI ACGGA 2 cut(s) 148, 1507
TspMI CCCGGG 1 cut(s) 253
TspRI CASTG 4 cut(s) 409, 421, 903, 1407
VpaK11BI GGWCC 4 cut(s) 163, 486, 561, 1688
XagI CCTNNNNNAGG 1 cut(s) 452
XapI RAATTY 3 cut(s) 805, 1315, 1711
XceI RCATGY 2 cut(s) 482, 1400
XcmI CCANNNNNNNNNTGG 1 cut(s) 1039
XhoI CTCGAG 1 cut(s) 347
XmaI CCCGGG 1 cut(s) 253
XmnI GAANNNNTTC 1 cut(s) 1713
XspI CTAG 2 cut(s) 645, 1191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.