MD02G1049400.v1.1

Component of the acetyl coenzyme A carboxylase (ACC) complex. Biotin carboxylase (BC) catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the transcarboxylase to acetyl-CoA to form malonyl- CoA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016119
Physical Location & Seq
Reverse (-)
3869977 .. 3870583
607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1049400.v1.1.491

Sequence Viewer

Length: 558 bp
ATGAGTAGTTTTGATAGAATTGAACTTTCGATTGATCCCGGTACTTGGGATCCTATGAACGAAGACATGGTCTCTCTAGATCCCATTGAATTTCATTCGGAGGAGGAACCTTATAAAGATTGTATTAATTCTTATAAAAAAAATACAGGATTAACTGAGGCTGTTCAAACAGGCACAGGTCAACTAAACTGTATGGGATCTGTGGTGGGCGAAAAAATCACACGTTTAGCCGAGTATGCTACCAATCAATTTTTACCTCTTATTCTAGTGTGTGCTTCCGGAGGAGCACGCATGCAAGAAGGAAGCTTGAGCTTGATGCAAATGGCTAAAATATCTTCCGCTTTATATGATTATCAATTAAATAAAAAGTTATTTTATGTAGCAATCCTTACATCCCCTACCACAGGCGAGGTGACGGCTAGTTTTGGTATGTTGGGAGATATCATTATTGCCGAACCCAATGCTTATATTGCATTTGCAGGTAAAAGAGTAATTGAACAAACATTGAATAAGACAGTACCTGAGGATTCACAAGTGGCTGAATATTTTATTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00195 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00195 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Carboxyl_trans PSI_8

Protein Analysis

186

Amino Acids

20.34

Weight (kDa)

4.46

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carboxyl_trans PF01039 5 - 178 8.2e-11 Carboxyl transferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 114, 135
Acc36I ACCTGC 1 cut(s) 470
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 1 cut(s) 339
AclWI GGATC 5 cut(s) 29, 44, 57, 74, 205
AcsI RAATTY 1 cut(s) 89
AfaI GTAC 2 cut(s) 43, 519
AfiI CCNNNNNNNGG 2 cut(s) 45, 404
AflIII ACRYGT 1 cut(s) 221
AgsI TTSAA 5 cut(s) 23, 89, 167, 497, 508
AluBI AGCT 2 cut(s) 306, 312
AluI AGCT 2 cut(s) 306, 312
Alw21I GWGCWC 1 cut(s) 289
Alw26I GTCTC 1 cut(s) 76
AlwI GGATC 5 cut(s) 29, 44, 57, 74, 205
AlwNI CAGNNNCTG 1 cut(s) 521
Aor13HI TCCGGA 1 cut(s) 278
ApoI RAATTY 1 cut(s) 89
ArsI GACNNNNNNTTYG 2 cut(s) 54, 86
AseI ATTAAT 1 cut(s) 126
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 1 cut(s) 424
AxyI CCTNAGG 1 cut(s) 522
BamHI GGATCC 1 cut(s) 49
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 289
BceAI ACGGC 1 cut(s) 432
BcgI CGANNNNNNTGC 2 cut(s) 443, 477
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 76
BfaI CTAG 3 cut(s) 77, 266, 420
BfuAI ACCTGC 1 cut(s) 470
Bme1390I CCNGG 1 cut(s) 39
BmiI GGNNCC 2 cut(s) 51, 108
BmrFI CCNGG 1 cut(s) 39
BmsI GCATC 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 328
BpuMI CCSGG 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 76
BsaWI WCCGGW 1 cut(s) 278
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 404
Bse21I CCTNAGG 1 cut(s) 522
BseAI TCCGGA 1 cut(s) 278
BseGI GGATG 1 cut(s) 392
BseLI CCNNNNNNNGG 2 cut(s) 45, 404
BseMII CTCAG 2 cut(s) 147, 513
BseRI GAGGAG 2 cut(s) 116, 297
BsiHKAI GWGCWC 1 cut(s) 289
BsiSI CCGG 2 cut(s) 39, 279
BslI CCNNNNNNNGG 2 cut(s) 45, 404
BsmAI GTCTC 1 cut(s) 76
Bso31I GGTCTC 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 289
Bsp13I TCCGGA 1 cut(s) 278
Bsp143I GATC 4 cut(s) 34, 49, 79, 197
BspACI CCGC 1 cut(s) 339
BspCNI CTCAG 2 cut(s) 148, 514
BspEI TCCGGA 1 cut(s) 278
BspLI GGNNCC 2 cut(s) 51, 108
BspMI ACCTGC 1 cut(s) 470
BspPI GGATC 5 cut(s) 29, 44, 57, 74, 205
BspTNI GGTCTC 1 cut(s) 76
BssMI GATC 4 cut(s) 34, 49, 79, 197
Bst4CI ACNGT 2 cut(s) 191, 517
BstC8I GCNNGC 2 cut(s) 289, 293
BstDEI CTNAG 2 cut(s) 156, 522
BstENI CCTNNNNNAGG 1 cut(s) 402
BstF5I GGATG 1 cut(s) 392
BstKTI GATC 4 cut(s) 37, 52, 82, 200
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 4 cut(s) 34, 49, 79, 197
BstMWI GCNNNNNNNGC 2 cut(s) 236, 470
BstNSI RCATGY 1 cut(s) 295
BstSCI CCNGG 1 cut(s) 37
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 3 cut(s) 49, 79, 197
BstYI RGATCY 3 cut(s) 49, 79, 197
Bsu36I CCTNAGG 1 cut(s) 522
BtsCI GGATG 1 cut(s) 392
BveI ACCTGC 1 cut(s) 470
Cac8I GCNNGC 2 cut(s) 289, 293
CaiI CAGNNNCTG 1 cut(s) 521
Csp6I GTAC 2 cut(s) 42, 518
CviAII CATG 2 cut(s) 67, 292
CviJI RGCY 7 cut(s) 161, 230, 306, 312, 326, 419, 539
CviKI_1 RGCY 7 cut(s) 161, 230, 306, 312, 326, 419, 539
CviQI GTAC 2 cut(s) 42, 518
DdeI CTNAG 2 cut(s) 156, 522
DpnI GATC 4 cut(s) 36, 51, 81, 199
DpnII GATC 4 cut(s) 34, 49, 79, 197
Eco31I GGTCTC 1 cut(s) 76
Eco32I GATATC 1 cut(s) 442
Eco81I CCTNAGG 1 cut(s) 522
EcoNI CCTNNNNNAGG 1 cut(s) 402
EcoRV GATATC 1 cut(s) 442
FaeI CATG 2 cut(s) 70, 295
FatI CATG 2 cut(s) 66, 291
FokI GGATG 1 cut(s) 379
FspBI CTAG 3 cut(s) 77, 266, 420
HapII CCGG 2 cut(s) 39, 279
Hin1II CATG 2 cut(s) 70, 295
HincII GTYRAC 1 cut(s) 182
HindII GTYRAC 1 cut(s) 182
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 1 cut(s) 527
HpaII CCGG 2 cut(s) 39, 279
HphI GGTGA 1 cut(s) 424
Hpy166II GTNNAC 1 cut(s) 182
Hpy188I TCNGA 1 cut(s) 100
Hpy188III TCNNGA 2 cut(s) 77, 279
Hpy8I GTNNAC 1 cut(s) 182
HpyAV CCTTC 1 cut(s) 293
HpyCH4III ACNGT 2 cut(s) 191, 517
HpyCH4IV ACGT 1 cut(s) 223
HpyCH4V TGCA 4 cut(s) 295, 319, 473, 479
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 470
HpyF3I CTNAG 2 cut(s) 156, 522
HpySE526I ACGT 1 cut(s) 223
Hsp92II CATG 2 cut(s) 70, 295
Kpn2I TCCGGA 1 cut(s) 278
Kzo9I GATC 4 cut(s) 34, 49, 79, 197
LmnI GCTCC 1 cut(s) 284
LpnPI CCDG 8 cut(s) 52, 132, 156, 162, 292, 390, 465, 534
LweI GCATC 1 cut(s) 306
MaeI CTAG 3 cut(s) 77, 266, 420
MaeII ACGT 1 cut(s) 223
MaeIII GTNAC 1 cut(s) 412
MalI GATC 4 cut(s) 36, 51, 81, 199
MboI GATC 4 cut(s) 34, 49, 79, 197
MboII GAAGA 2 cut(s) 74, 327
MflI RGATCY 3 cut(s) 49, 79, 197
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 6 cut(s) 18, 89, 127, 248, 356, 492
MnlI CCTC 7 cut(s) 94, 97, 151, 267, 275, 403, 517
MroI TCCGGA 1 cut(s) 278
MseI TTAA 3 cut(s) 126, 152, 359
MspI CCGG 2 cut(s) 39, 279
MspR9I CCNGG 1 cut(s) 39
MwoI GCNNNNNNNGC 2 cut(s) 236, 470
NciI CCSGG 1 cut(s) 39
NdeII GATC 4 cut(s) 34, 49, 79, 197
NlaIII CATG 2 cut(s) 70, 295
NlaIV GGNNCC 2 cut(s) 51, 108
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 412
NspI RCATGY 1 cut(s) 295
PaeI GCATGC 1 cut(s) 295
PfeI GAWTC 1 cut(s) 527
PflFI GACNNNGTC 1 cut(s) 68
PshBI ATTAAT 1 cut(s) 126
PsiI TTATAA 2 cut(s) 114, 135
PspN4I GGNNCC 2 cut(s) 51, 108
PstNI CAGNNNCTG 1 cut(s) 521
PsuI RGATCY 3 cut(s) 49, 79, 197
PsyI GACNNNGTC 1 cut(s) 68
RsaI GTAC 2 cut(s) 43, 519
RsaNI GTAC 2 cut(s) 42, 518
SaqAI TTAA 3 cut(s) 126, 152, 359
Sau3AI GATC 4 cut(s) 34, 49, 79, 197
ScrFI CCNGG 1 cut(s) 39
SduI GDGCHC 1 cut(s) 289
SetI ASST 9 cut(s) 112, 181, 226, 259, 308, 314, 414, 484, 523
SfaNI GCATC 1 cut(s) 306
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
SphI GCATGC 1 cut(s) 295
Sse9I AATT 6 cut(s) 18, 89, 127, 248, 356, 492
SsiI CCGC 1 cut(s) 339
SspI AATATT 1 cut(s) 545
SspMI CTAG 3 cut(s) 77, 266, 420
StyD4I CCNGG 1 cut(s) 37
TaaI ACNGT 2 cut(s) 191, 517
TaiI ACGT 1 cut(s) 226
TaqI TCGA 1 cut(s) 29
TasI AATT 6 cut(s) 18, 89, 127, 248, 356, 492
TfiI GAWTC 1 cut(s) 527
Tru1I TTAA 3 cut(s) 126, 152, 359
Tru9I TTAA 3 cut(s) 126, 152, 359
TseFI GTSAC 1 cut(s) 412
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 2 cut(s) 71, 83
Tth111I GACNNNGTC 1 cut(s) 68
VspI ATTAAT 1 cut(s) 126
XagI CCTNNNNNAGG 1 cut(s) 402
XapI RAATTY 1 cut(s) 89
XbaI TCTAGA 1 cut(s) 76
XceI RCATGY 1 cut(s) 295
XspI CTAG 3 cut(s) 77, 266, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.