MD02G1213300.v1.1

Component of the acetyl coenzyme A carboxylase (ACC) complex. Biotin carboxylase (BC) catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the transcarboxylase to acetyl-CoA to form malonyl- CoA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016119
Physical Location & Seq
Reverse (-)
23240156 .. 23240715
560 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1213300.v1.1.491

Sequence Viewer

Length: 297 bp
ATGAGTAGTTTTGATAGAATCGAACTTTCGATTGATCCCGGTACCTGGGATCCTATGAACGAAGACATGGCTGTTCAAACAGGCATAGGTCAACTAAATGGTATTCCCGTAGCAATTGGTGTTATGGATTTTCAGTTTATGGGTGGTAGTATGGGATCTGCAGTGGCAATCCTTACATCCCCTACCACAGGCAGGGTGACGGCTAGTTTTGGTATGTTGGGAGATATCATTATTGCCGAACCCAATGCTTATATTGCATTTGCAGGTAAAAGAGTAATTGAACAAAACATTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00195 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00195 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Carboxyl_trans PSI_8

Protein Analysis

99

Amino Acids

10.35

Weight (kDa)

4.12

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 254
Acc65I GGTACC 1 cut(s) 41
AccB1I GGYRCC 1 cut(s) 41
AclWI GGATC 4 cut(s) 29, 44, 57, 163
AfaI GTAC 1 cut(s) 43
AfiI CCNNNNNNNGG 3 cut(s) 45, 188, 192
AgsI TTSAA 3 cut(s) 77, 281, 293
AjnI CCWGG 1 cut(s) 44
AlwI GGATC 4 cut(s) 29, 44, 57, 163
Asp718I GGTACC 1 cut(s) 41
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 1 cut(s) 208
BamHI GGATCC 1 cut(s) 49
BanI GGYRCC 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 69
BceAI ACGGC 1 cut(s) 216
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciT130I CCWGG 1 cut(s) 46
BcnI CCSGG 1 cut(s) 39
BfaI CTAG 1 cut(s) 204
BfmI CTRYAG 1 cut(s) 159
BfuAI ACCTGC 1 cut(s) 254
Bme1390I CCNGG 2 cut(s) 39, 46
BmiI GGNNCC 2 cut(s) 43, 51
BmrFI CCNGG 2 cut(s) 39, 46
BpiI GAAGAC 1 cut(s) 69
BpuMI CCSGG 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 45
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 188, 192
BseBI CCWGG 1 cut(s) 46
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 1 cut(s) 176
BseLI CCNNNNNNNGG 3 cut(s) 45, 188, 192
BshNI GGYRCC 1 cut(s) 41
BsiSI CCGG 1 cut(s) 39
BslI CCNNNNNNNGG 3 cut(s) 45, 188, 192
Bsp143I GATC 3 cut(s) 34, 49, 155
BspLI GGNNCC 2 cut(s) 43, 51
BspMAI CTGCAG 1 cut(s) 163
BspMI ACCTGC 1 cut(s) 254
BspPI GGATC 4 cut(s) 29, 44, 57, 163
BspT107I GGYRCC 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 3 cut(s) 34, 49, 155
Bst2UI CCWGG 1 cut(s) 46
BstENI CCTNNNNNAGG 1 cut(s) 186
BstF5I GGATG 1 cut(s) 176
BstKTI GATC 3 cut(s) 37, 52, 158
BstMBI GATC 3 cut(s) 34, 49, 155
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 1 cut(s) 46
BstSCI CCNGG 2 cut(s) 37, 44
BstSFI CTRYAG 1 cut(s) 159
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 2 cut(s) 49, 155
BstYI RGATCY 2 cut(s) 49, 155
BtsCI GGATG 1 cut(s) 176
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 1 cut(s) 168
BveI ACCTGC 1 cut(s) 254
Csp6I GTAC 1 cut(s) 42
CviAII CATG 1 cut(s) 67
CviJI RGCY 2 cut(s) 71, 203
CviKI_1 RGCY 2 cut(s) 71, 203
CviQI GTAC 1 cut(s) 42
DpnI GATC 3 cut(s) 36, 51, 157
DpnII GATC 3 cut(s) 34, 49, 155
Eco32I GATATC 1 cut(s) 226
EcoNI CCTNNNNNAGG 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 44
EcoRV GATATC 1 cut(s) 226
FaeI CATG 1 cut(s) 70
FaiI YATR 8 cut(s) 56, 68, 86, 125, 140, 152, 215, 252
FatI CATG 1 cut(s) 66
FokI GGATG 1 cut(s) 163
FspBI CTAG 1 cut(s) 204
HapII CCGG 1 cut(s) 39
Hin1II CATG 1 cut(s) 70
HincII GTYRAC 1 cut(s) 92
HindII GTYRAC 1 cut(s) 92
HinfI GANTC 1 cut(s) 18
HpaII CCGG 1 cut(s) 39
HphI GGTGA 1 cut(s) 208
Hpy166II GTNNAC 1 cut(s) 92
Hpy8I GTNNAC 1 cut(s) 92
HpyCH4V TGCA 3 cut(s) 161, 257, 263
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
Hsp92II CATG 1 cut(s) 70
KpnI GGTACC 1 cut(s) 45
Kzo9I GATC 3 cut(s) 34, 49, 155
LpnPI CCDG 7 cut(s) 31, 52, 58, 66, 174, 178, 249
MaeI CTAG 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 196
MalI GATC 3 cut(s) 36, 51, 157
MboI GATC 3 cut(s) 34, 49, 155
MboII GAAGA 1 cut(s) 74
MfeI CAATTG 1 cut(s) 114
MflI RGATCY 2 cut(s) 49, 155
MluCI AATT 2 cut(s) 114, 276
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 2 cut(s) 39, 46
MunI CAATTG 1 cut(s) 114
MvaI CCWGG 1 cut(s) 46
MwoI GCNNNNNNNGC 1 cut(s) 254
NciI CCSGG 1 cut(s) 39
NdeII GATC 3 cut(s) 34, 49, 155
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 2 cut(s) 43, 51
NmuCI GTSAC 1 cut(s) 196
PfeI GAWTC 1 cut(s) 18
Psp6I CCWGG 1 cut(s) 44
PspGI CCWGG 1 cut(s) 44
PspN4I GGNNCC 2 cut(s) 43, 51
PstI CTGCAG 1 cut(s) 163
PsuI RGATCY 2 cut(s) 49, 155
RsaI GTAC 1 cut(s) 43
RsaNI GTAC 1 cut(s) 42
Sau3AI GATC 3 cut(s) 34, 49, 155
ScrFI CCNGG 2 cut(s) 39, 46
SetI ASST 3 cut(s) 47, 91, 268
SfcI CTRYAG 1 cut(s) 159
Sse9I AATT 2 cut(s) 114, 276
SspMI CTAG 1 cut(s) 204
StyD4I CCNGG 2 cut(s) 37, 44
TaqI TCGA 2 cut(s) 21, 29
TasI AATT 2 cut(s) 114, 276
TfiI GAWTC 1 cut(s) 18
TscAI CASTG 1 cut(s) 168
TseFI GTSAC 1 cut(s) 196
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 1 cut(s) 71
TspRI CASTG 1 cut(s) 168
XagI CCTNNNNNAGG 1 cut(s) 186
XspI CTAG 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.