MD02G1065400.v1.1

Senescence-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006695
Physical Location & Seq
Reverse (-)
5303059 .. 5305603
2545 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1065400.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGGGTTGCTTCAGTCGTTCACACCGTTCCAAACCCAAAACCACACCTCCGCCGCAGTACGGAACCCAAGACCCTACTGAGCAATTCCAACAACCCAAAAACATCCAGCAGCAAGTCTTCCTCCAAATCCCAGGATGCAGAGTCAACCTCATGGACGAAGGAGAAACCCTAGAGCTCGCAAGCGGCGACTTCGTCCTCGAAAACATCTTGGAAAACAACGTTACTCTGGCCACCATAATCAAAGTCGGCGAAGAACTTCAGTGGCCGCTGACAAACGACGAGCCGGTCGTGAAGCTCGACGCTTTGCACTACCTCTTCTCCCTCCCCATGAAGGACGGCGATCCGCTGAGCTACGGTGTCACTTTTCCCGACCAGTGTGAGAGTCAGTTGGCGTTTCTGGATTCGTTTTTGATGGAGCACACTTGCTTTAGTGCCACATCGACCAAGAATAATAAAAATAAGGGGGTTGATTGGAAGGAGTATGCTCCAAGGATTGAAGATTATAATAATGTGTTGGCCAAGGCGATTGCGGGAGGGACTGGTCAGATTGTGAGGGGGATCTTCATGTGTAGCAATGCTTACACCAATCAGGTCCAAAAGGGAGGGGAAGCGACATTGGCTCGTCCCGTGGAGGGGAAAAGTTATGTCAAAGAACAAGGAAGCAGTAGCAGCAAATCTGCTGCAAAAAAGAAGAGTGGAATCAACAAGAACTTAAAACGTGTGAAAAATTTGTCAAAGATGACATCAAAGCTCAGCAAAGCTATGCTTGATGGGGTTGGTATTGCGACCGGATCGGTGATGGGGCCGGTAGTCAACTCCAAAGCAGGGAAGTCATTCTTCGCCTTGGTTCCGGGACAGGTCCTCTTGGCTTCACTTGATGCCATCAATAAGATTTTGGATGCAGCTGAAGTTGCAGAGAAACAAGCCCTATCAGCTACCAAGGGTGCTGCTGGTAGAATCGTCAGCAACAGGTTTGGGGAGAGTGCAGGGGAGGCAACTGAGGATGTGATTGCAACAGCGGGGCATTGTGCTTGCATTGCTTGGAACGTCTTCAAAATTAGAAAGGCCATCAATCCAGCATCATCTGTCAAAACAGGAGTGCTCAAGAATGCTGTCAGAAAAGGAGTCAAGAATGCTGTCAAAAATAGAAGCAAAGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Senescence

Protein Analysis

388

Amino Acids

41.68

Weight (kDa)

9.24

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Senescence PF06911 174 - 371 2.4e-34 Senescence domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 504
AasI GACNNNNNNGTC 1 cut(s) 284
AciI CCGC 7 cut(s) 50, 53, 183, 266, 344, 530, 1019
AclI AACGTT 1 cut(s) 219
AclWI GGATC 3 cut(s) 335, 566, 799
AcoI YGGCCR 3 cut(s) 228, 263, 516
AcsI RAATTY 1 cut(s) 727
AcuI CTGAAG 2 cut(s) 242, 927
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 5 cut(s) 59, 331, 632, 633, 825
AflIII ACRYGT 1 cut(s) 718
AgsI TTSAA 2 cut(s) 497, 1054
AjnI CCWGG 1 cut(s) 130
AluBI AGCT 7 cut(s) 175, 295, 351, 751, 761, 905, 935
AluI AGCT 7 cut(s) 175, 295, 351, 751, 761, 905, 935
Alw21I GWGCWC 3 cut(s) 177, 420, 1104
AlwI GGATC 3 cut(s) 335, 566, 799
AoxI GGCC 5 cut(s) 228, 263, 516, 803, 1065
ApeKI GCWGC 5 cut(s) 109, 669, 680, 902, 947
ApoI RAATTY 1 cut(s) 727
Asp700I GAANNNNTTC 3 cut(s) 255, 833, 1049
AspS9I GGNCC 3 cut(s) 592, 803, 859
AsuC2I CCSGG 1 cut(s) 852
AsuHPI GGTGA 1 cut(s) 808
AvaII GGWCC 2 cut(s) 592, 859
BalI TGGCCA 2 cut(s) 230, 518
BanII GRGCYC 1 cut(s) 177
BbsI GAAGAC 2 cut(s) 109, 1042
Bbv12I GWGCWC 3 cut(s) 177, 420, 1104
BbvI GCAGC 5 cut(s) 121, 667, 681, 914, 934
BccI CCATC 5 cut(s) 406, 764, 793, 890, 1076
BceAI ACGGC 1 cut(s) 352
BciT130I CCWGG 1 cut(s) 132
BcnI CCSGG 1 cut(s) 852
BfaI CTAG 1 cut(s) 170
BisI GCNGC 8 cut(s) 53, 110, 184, 266, 670, 681, 903, 948
BlpI GCTNAGC 2 cut(s) 347, 752
BlsI GCNGC 8 cut(s) 54, 111, 185, 267, 671, 682, 904, 949
Bme1390I CCNGG 2 cut(s) 132, 852
Bme18I GGWCC 2 cut(s) 592, 859
BmgT120I GGNCC 3 cut(s) 592, 803, 859
BmiI GGNNCC 3 cut(s) 64, 804, 849
BmrFI CCNGG 2 cut(s) 132, 852
BmsI GCATC 4 cut(s) 125, 868, 889, 1088
BpiI GAAGAC 2 cut(s) 109, 1042
BplI GAGNNNNNCTC 2 cut(s) 132, 164
Bpu1102I GCTNAGC 2 cut(s) 347, 752
BpuEI CTTGAG 1 cut(s) 1088
BpuMI CCSGG 1 cut(s) 852
BsaJI CCNNGG 6 cut(s) 130, 488, 519, 627, 843, 939
BsaWI WCCGGW 1 cut(s) 788
BsaXI ACNNNNNCTCC 4 cut(s) 31, 61, 302, 332
Bsc4I CCNNNNNNNGG 5 cut(s) 59, 331, 632, 633, 825
Bse118I RCCGGY 2 cut(s) 283, 805
Bse1I ACTGG 2 cut(s) 373, 544
Bse3DI GCAATG 2 cut(s) 580, 1035
BseBI CCWGG 1 cut(s) 132
BseDI CCNNGG 6 cut(s) 130, 488, 519, 627, 843, 939
BseGI GGATG 4 cut(s) 102, 140, 904, 1009
BseLI CCNNNNNNNGG 5 cut(s) 59, 331, 632, 633, 825
BseMI GCAATG 2 cut(s) 580, 1035
BseMII CTCAG 4 cut(s) 69, 338, 766, 990
BseNI ACTGG 2 cut(s) 373, 544
BseXI GCAGC 5 cut(s) 121, 667, 681, 914, 934
BsgI GTGCAG 1 cut(s) 1005
Bsh1285I CGRYCG 2 cut(s) 288, 789
BshFI GGCC 5 cut(s) 230, 265, 518, 805, 1067
BsiEI CGRYCG 2 cut(s) 288, 789
BsiHKAI GWGCWC 3 cut(s) 177, 420, 1104
BsiSI CCGG 4 cut(s) 284, 789, 806, 851
BslFI GGGAC 3 cut(s) 550, 609, 867
BslI CCNNNNNNNGG 5 cut(s) 59, 331, 632, 633, 825
BsmFI GGGAC 3 cut(s) 550, 609, 867
BsmI GAATGC 2 cut(s) 1114, 1138
BsnI GGCC 5 cut(s) 230, 265, 518, 805, 1067
Bsp1286I GDGCHC 3 cut(s) 177, 420, 1104
Bsp143I GATC 3 cut(s) 340, 558, 791
Bsp1720I GCTNAGC 2 cut(s) 347, 752
BspACI CCGC 7 cut(s) 50, 53, 183, 266, 344, 530, 1019
BspANI GGCC 5 cut(s) 230, 265, 518, 805, 1067
BspCNI CTCAG 4 cut(s) 70, 339, 765, 991
BspLI GGNNCC 3 cut(s) 64, 804, 849
BspPI GGATC 3 cut(s) 335, 566, 799
BsrDI GCAATG 2 cut(s) 580, 1035
BsrFI RCCGGY 2 cut(s) 283, 805
BsrI ACTGG 2 cut(s) 373, 544
BssAI RCCGGY 2 cut(s) 283, 805
BssECI CCNNGG 6 cut(s) 130, 488, 519, 627, 843, 939
BssMI GATC 3 cut(s) 340, 558, 791
BssT1I CCWWGG 4 cut(s) 488, 519, 843, 939
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 2 cut(s) 26, 356
Bst6I CTCTTC 2 cut(s) 320, 686
BstC8I GCNNGC 3 cut(s) 177, 181, 1033
BstDEI CTNAG 4 cut(s) 78, 347, 752, 999
BstDSI CCRYGG 1 cut(s) 627
BstF5I GGATG 4 cut(s) 102, 140, 904, 1009
BstKTI GATC 3 cut(s) 343, 561, 794
BstMBI GATC 3 cut(s) 340, 558, 791
BstMCI CGRYCG 2 cut(s) 288, 789
BstMWI GCNNNNNNNGC 6 cut(s) 617, 669, 911, 932, 992, 1037
BstNI CCWGG 1 cut(s) 132
BstSCI CCNGG 2 cut(s) 130, 850
BstV1I GCAGC 5 cut(s) 121, 667, 681, 914, 934
BstV2I GAAGAC 2 cut(s) 109, 1042
BstX2I RGATCY 1 cut(s) 558
BstYI RGATCY 1 cut(s) 558
BsuRI GGCC 5 cut(s) 230, 265, 518, 805, 1067
BtgI CCRYGG 1 cut(s) 627
BtsCI GGATG 4 cut(s) 102, 140, 904, 1009
BtsIMutI CAGTG 2 cut(s) 266, 380
Cac8I GCNNGC 3 cut(s) 177, 181, 1033
Cfr10I RCCGGY 2 cut(s) 283, 805
Cfr13I GGNCC 3 cut(s) 592, 803, 859
CseI GACGC 1 cut(s) 308
Csp6I GTAC 1 cut(s) 58
CviAII CATG 3 cut(s) 151, 328, 565
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 4 cut(s) 78, 347, 752, 999
DpnI GATC 3 cut(s) 342, 560, 793
DpnII GATC 3 cut(s) 340, 558, 791
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
EaeI YGGCCR 3 cut(s) 228, 263, 516
Eam1104I CTCTTC 2 cut(s) 320, 686
EarI CTCTTC 2 cut(s) 320, 686
EciI GGCGGA 1 cut(s) 39
Ecl136II GAGCTC 1 cut(s) 175
Eco130I CCWWGG 4 cut(s) 488, 519, 843, 939
Eco24I GRGCYC 1 cut(s) 177
Eco47I GGWCC 2 cut(s) 592, 859
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 2 cut(s) 242, 927
EcoICRI GAGCTC 1 cut(s) 175
EcoO109I RGGNCCY 1 cut(s) 859
EcoRII CCWGG 1 cut(s) 130
EcoT14I CCWWGG 4 cut(s) 488, 519, 843, 939
EcoT38I GRGCYC 1 cut(s) 177
ErhI CCWWGG 4 cut(s) 488, 519, 843, 939
FaeI CATG 3 cut(s) 154, 331, 568
FaiI YATR 8 cut(s) 152, 236, 329, 483, 504, 566, 645, 764
FalI AAGNNNNNCTT 4 cut(s) 750, 782, 821, 853
FaqI GGGAC 3 cut(s) 550, 609, 867
FatI CATG 3 cut(s) 150, 327, 564
FauI CCCGC 2 cut(s) 523, 1012
Fnu4HI GCNGC 8 cut(s) 53, 110, 184, 266, 670, 681, 903, 948
FokI GGATG 4 cut(s) 89, 147, 911, 1016
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 8 cut(s) 53, 110, 184, 266, 670, 681, 903, 948
FspBI CTAG 1 cut(s) 170
GluI GCNGC 8 cut(s) 53, 110, 184, 266, 670, 681, 903, 948
HaeIII GGCC 5 cut(s) 230, 265, 518, 805, 1067
HapII CCGG 4 cut(s) 284, 789, 806, 851
HgaI GACGC 1 cut(s) 308
Hin1II CATG 3 cut(s) 154, 331, 568
HincII GTYRAC 2 cut(s) 145, 814
HindII GTYRAC 2 cut(s) 145, 814
HinfI GANTC 7 cut(s) 141, 382, 401, 699, 957, 1125, 1157
HpaII CCGG 4 cut(s) 284, 789, 806, 851
HphI GGTGA 1 cut(s) 808
Hpy166II GTNNAC 3 cut(s) 20, 145, 814
Hpy188I TCNGA 2 cut(s) 546, 1118
Hpy188III TCNNGA 5 cut(s) 289, 368, 398, 1105, 1129
Hpy8I GTNNAC 3 cut(s) 20, 145, 814
Hpy99I CGWCG 2 cut(s) 281, 302
HpyAV CCTTC 3 cut(s) 152, 325, 469
HpyCH4III ACNGT 2 cut(s) 26, 356
HpyCH4IV ACGT 3 cut(s) 219, 718, 1047
HpyCH4V TGCA 8 cut(s) 138, 307, 683, 902, 914, 986, 1013, 1035
HpyF10VI GCNNNNNNNGC 6 cut(s) 617, 669, 911, 932, 992, 1037
HpyF3I CTNAG 4 cut(s) 78, 347, 752, 999
HpySE526I ACGT 3 cut(s) 219, 718, 1047
Hsp92II CATG 3 cut(s) 154, 331, 568
Kzo9I GATC 3 cut(s) 340, 558, 791
LmnI GCTCC 2 cut(s) 415, 490
Lsp1109I GCAGC 5 cut(s) 121, 667, 681, 914, 934
LweI GCATC 4 cut(s) 125, 868, 889, 1088
MaeI CTAG 1 cut(s) 170
MaeII ACGT 3 cut(s) 219, 718, 1047
MaeIII GTNAC 2 cut(s) 220, 358
MalI GATC 3 cut(s) 342, 560, 793
MboI GATC 3 cut(s) 340, 558, 791
MboII GAAGA 8 cut(s) 109, 263, 307, 509, 553, 703, 829, 1042
MflI RGATCY 1 cut(s) 558
MhlI GDGCHC 3 cut(s) 177, 420, 1104
MlsI TGGCCA 2 cut(s) 230, 518
MluCI AATT 3 cut(s) 83, 727, 1056
MluNI TGGCCA 2 cut(s) 230, 518
MlyI GAGTC 3 cut(s) 150, 391, 1134
MmeI TCCRAC 1 cut(s) 112
Mox20I TGGCCA 2 cut(s) 230, 518
MroXI GAANNNNTTC 3 cut(s) 255, 833, 1049
MscI TGGCCA 2 cut(s) 230, 518
MseI TTAA 2 cut(s) 713, 1162
Msp20I TGGCCA 2 cut(s) 230, 518
MspA1I CMGCKG 4 cut(s) 268, 346, 905, 1019
MspI CCGG 4 cut(s) 284, 789, 806, 851
MspR9I CCNGG 2 cut(s) 132, 852
Mva1269I GAATGC 2 cut(s) 1114, 1138
MvaI CCWGG 1 cut(s) 132
MwoI GCNNNNNNNGC 6 cut(s) 617, 669, 911, 932, 992, 1037
NciI CCSGG 1 cut(s) 852
NdeII GATC 3 cut(s) 340, 558, 791
NlaIII CATG 3 cut(s) 154, 331, 568
NlaIV GGNNCC 3 cut(s) 64, 804, 849
NmuCI GTSAC 1 cut(s) 358
PcsI WCGNNNNNNNCGW 4 cut(s) 22, 183, 285, 294
PctI GAATGC 2 cut(s) 1114, 1138
PdmI GAANNNNTTC 3 cut(s) 255, 833, 1049
PfeI GAWTC 4 cut(s) 401, 699, 957, 1157
PflFI GACNNNGTC 1 cut(s) 191
PfoI TCCNGGA 1 cut(s) 850
PkrI GCNGC 8 cut(s) 54, 111, 185, 267, 671, 682, 904, 949
PleI GAGTC 3 cut(s) 149, 390, 1133
PpsI GAGTC 3 cut(s) 149, 390, 1133
PpuMI RGGWCCY 1 cut(s) 859
PsiI TTATAA 1 cut(s) 504
Psp124BI GAGCTC 1 cut(s) 177
Psp1406I AACGTT 1 cut(s) 219
Psp5II RGGWCCY 1 cut(s) 859
Psp6I CCWGG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 3 cut(s) 64, 804, 849
PspPI GGNCC 3 cut(s) 592, 803, 859
PspPPI RGGWCCY 1 cut(s) 859
PsuI RGATCY 1 cut(s) 558
PsyI GACNNNGTC 1 cut(s) 191
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
SacI GAGCTC 1 cut(s) 177
SaqAI TTAA 2 cut(s) 713, 1162
SatI GCNGC 8 cut(s) 53, 110, 184, 266, 670, 681, 903, 948
Sau3AI GATC 3 cut(s) 340, 558, 791
Sau96I GGNCC 3 cut(s) 592, 803, 859
SchI GAGTC 3 cut(s) 150, 391, 1134
ScrFI CCNGG 2 cut(s) 132, 852
SduI GDGCHC 3 cut(s) 177, 420, 1104
SfaNI GCATC 4 cut(s) 125, 868, 889, 1088
SinI GGWCC 2 cut(s) 592, 859
SmlI CTYRAG 1 cut(s) 1103
SmoI CTYRAG 1 cut(s) 1103
Sse9I AATT 3 cut(s) 83, 727, 1056
SsiI CCGC 7 cut(s) 50, 53, 183, 266, 344, 530, 1019
SspMI CTAG 1 cut(s) 170
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 2 cut(s) 130, 850
StyI CCWWGG 4 cut(s) 488, 519, 843, 939
TaaI ACNGT 2 cut(s) 26, 356
TaiI ACGT 3 cut(s) 222, 721, 1050
TaqI TCGA 3 cut(s) 198, 297, 440
TasI AATT 3 cut(s) 83, 727, 1056
TauI GCSGC 3 cut(s) 55, 186, 268
TfiI GAWTC 4 cut(s) 401, 699, 957, 1157
Tru1I TTAA 2 cut(s) 713, 1162
Tru9I TTAA 2 cut(s) 713, 1162
TscAI CASTG 2 cut(s) 266, 380
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 5 cut(s) 109, 669, 680, 902, 947
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 2 cut(s) 344, 553
TspGWI ACGGA 1 cut(s) 75
TspRI CASTG 2 cut(s) 266, 380
Tth111I GACNNNGTC 1 cut(s) 191
VpaK11BI GGWCC 2 cut(s) 592, 859
XapI RAATTY 1 cut(s) 727
XmnI GAANNNNTTC 3 cut(s) 255, 833, 1049
XspI CTAG 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.