MD02G1076300.v1.1
MYB Family

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014417
Physical Location & Seq
Forward (+)
6040862 .. 6042593
1732 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1076300.v1.1.491

Sequence Viewer

Length: 966 bp
ATGGGCAGAAAGTGCTCACATTGTGGAAACATAGGCCACAACTCTAGGACCTGCGCAAATTTCAGAGGCACTACTAGTAGTACTAGTTTTGTTGGATTTAGGCTCTTTGGTGTCCAACTTCATCATGATCATGATCATCATATCATGATGTCTTCATCTAACATGAACAATAATTCTGCTAATTATCATGTTTCCATGCCCATGAAGAAAAGTTTCAGCATGGATTGCTTGCCCACGACGACGTCGTCAGCCTCTTCATCTTCCCCATCTAATTCTTCATTGTCTCCTTCAAGGCTTTCCATGGATGAAAATATTTGTGCATCAGATAAAGCCTCCATCGGCTATCTCTCTGACGGTCTCATATGCCGAGCCCAAGAAAGGAAGAAGGGAGTTCCATGGACGGAAGAGGAGCACCGGACGTTCCTAATCGGACTCGAGAAGTTAGGAAAGGGTGACTGGAGAGGTATCTCTAGGAATTATGTGACCACAAGAACCCCTACTCAAGTTGCGAGTCATGCTCAGAAGTATTTTCTCAGGCAAGCAAGCCTGACCAAAAAGAAGCGACGTTCTAGCCTCTTTGATATGTTTGGAAGCAGCAACAATATAAATATGGAAACTGATGATCCAATTCCCACTCAACGTGACAATATTATTCGTAATAACATGGCCATTGATTCCAGCACAGTGCCTCTGCTTGAACATAGCATTGCAGCGAAAAATCTAAACTTAGATCACAGTCAAAAACCTCACAGCAGTAGTACTACTACTCGGCAGCTTCCGGTTTGGATTTACGAGTTGACCGATTCCCAGATGAAACCGAATTCATCTTCGAGTAAAGCGTCAGTAGTACCGGATCTAGAGCTCACGCTTGCTGCACCAACTAGTCCTAATGTAGAACAAAAAAATAAATCATCCCCAAGCCCAGCAGGTCCCCTCCTTAATCTTGGAACAATTAGTGTCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

322

Amino Acids

35.28

Weight (kDa)

9.31

Isoelectric Point (pI)

54.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 131 - 177 7e-11 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014417)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56840
fragaria_vesca FvH4_1g07170 FvH4_1g07170
malus_domestica MD02G1076300.v1.1 MD15G1204700.v1.1
prunus_persica Prupe.7G212300_v2.0.a1
pyrus_communis pycom02g06060 pycom15g18170
rosa_chinensis RchiOBHm_Chr2g0093071
rosa_laevigata RLG00000016365
rosa_roxburghii Rroxscaffold_2G00148540
rosa_rugosa Rorug02G0032200
rosa_samantha Rh2AG077700 Rh2BG079100 Rh2CG081500 Rh2DG076600
rosa_wichuraiana Rw2G006850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 244
AatII GACGTC 1 cut(s) 245
Acc16I TGCGCA 1 cut(s) 55
Acc36I ACCTGC 2 cut(s) 59, 917
AclWI GGATC 2 cut(s) 617, 861
AcoI YGGCCR 1 cut(s) 666
AcsI RAATTY 2 cut(s) 58, 820
AcyI GRCGYC 1 cut(s) 242
AdeI CACNNNGTG 1 cut(s) 23
AfaI GTAC 3 cut(s) 82, 760, 849
AfiI CCNNNNNNNGG 1 cut(s) 378
AgsI TTSAA 2 cut(s) 291, 698
AhlI ACTAGT 3 cut(s) 74, 83, 881
AluBI AGCT 2 cut(s) 775, 862
AluI AGCT 2 cut(s) 775, 862
Alw21I GWGCWC 3 cut(s) 17, 414, 864
Alw26I GTCTC 2 cut(s) 288, 362
AlwI GGATC 2 cut(s) 617, 861
Ama87I CYCGRG 1 cut(s) 434
AoxI GGCC 2 cut(s) 34, 666
ApeKI GCWGC 4 cut(s) 594, 710, 772, 872
ApoI RAATTY 2 cut(s) 58, 820
Asp700I GAANNNNTTC 1 cut(s) 212
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 2 cut(s) 48, 929
AsuHPI GGTGA 1 cut(s) 464
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 2 cut(s) 48, 929
BalI TGGCCA 1 cut(s) 668
BanII GRGCYC 2 cut(s) 373, 864
BbsI GAAGAC 1 cut(s) 144
Bbv12I GWGCWC 3 cut(s) 17, 414, 864
BbvI GCAGC 4 cut(s) 606, 722, 784, 859
BccI CCATC 2 cut(s) 274, 344
BclI TGATCA 2 cut(s) 127, 133
BcoDI GTCTC 2 cut(s) 288, 362
BcuI ACTAGT 3 cut(s) 74, 83, 881
BfaI CTAG 7 cut(s) 45, 75, 84, 471, 570, 857, 882
BfuAI ACCTGC 2 cut(s) 59, 917
BisI GCNGC 4 cut(s) 595, 711, 773, 873
BlsI GCNGC 4 cut(s) 596, 712, 774, 874
BmcAI AGTACT 2 cut(s) 82, 760
Bme18I GGWCC 2 cut(s) 48, 929
BmeT110I CYCGRG 1 cut(s) 434
BmgT120I GGNCC 2 cut(s) 48, 929
BmiI GGNNCC 1 cut(s) 931
BmsI GCATC 1 cut(s) 329
BpiI GAAGAC 1 cut(s) 144
BplI GAGNNNNNCTC 2 cut(s) 502, 534
BpmI CTGGAG 1 cut(s) 478
BpuEI CTTGAG 1 cut(s) 486
BsaBI GATNNNNATC 1 cut(s) 132
BsaHI GRCGYC 1 cut(s) 242
BsaI GGTCTC 1 cut(s) 362
BsaJI CCNNGG 2 cut(s) 300, 395
BsaWI WCCGGW 3 cut(s) 414, 778, 850
Bsc4I CCNNNNNNNGG 1 cut(s) 378
Bse1I ACTGG 1 cut(s) 461
Bse3DI GCAATG 1 cut(s) 705
Bse8I GATNNNNATC 1 cut(s) 132
BseDI CCNNGG 2 cut(s) 300, 395
BseGI GGATG 2 cut(s) 310, 911
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 1 cut(s) 378
BseMI GCAATG 1 cut(s) 705
BseMII CTCAG 2 cut(s) 533, 547
BseNI ACTGG 1 cut(s) 461
BseRI GAGGAG 1 cut(s) 422
BseXI GCAGC 4 cut(s) 606, 722, 784, 859
BseYI CCCAGC 1 cut(s) 922
BsgI GTGCAG 1 cut(s) 858
BshFI GGCC 2 cut(s) 36, 668
BsiHKAI GWGCWC 3 cut(s) 17, 414, 864
BsiHKCI CYCGRG 1 cut(s) 434
BsiSI CCGG 3 cut(s) 415, 779, 851
BslFI GGGAC 1 cut(s) 915
BslI CCNNNNNNNGG 1 cut(s) 378
BsmAI GTCTC 2 cut(s) 288, 362
BsmFI GGGAC 1 cut(s) 915
BsnI GGCC 2 cut(s) 36, 668
Bso31I GGTCTC 1 cut(s) 362
BsoBI CYCGRG 1 cut(s) 434
Bsp1286I GDGCHC 4 cut(s) 17, 373, 414, 864
Bsp143I GATC 5 cut(s) 127, 133, 622, 730, 853
Bsp19I CCATGG 2 cut(s) 300, 395
BspANI GGCC 2 cut(s) 36, 668
BspCNI CTCAG 2 cut(s) 532, 546
BspHI TCATGA 3 cut(s) 124, 130, 144
BspLI GGNNCC 1 cut(s) 931
BspMI ACCTGC 2 cut(s) 59, 917
BspPI GGATC 2 cut(s) 617, 861
BspTNI GGTCTC 1 cut(s) 362
BsrDI GCAATG 1 cut(s) 705
BsrI ACTGG 1 cut(s) 461
BssECI CCNNGG 2 cut(s) 300, 395
BssMI GATC 5 cut(s) 127, 133, 622, 730, 853
BssNI GRCGYC 1 cut(s) 242
BssT1I CCWWGG 2 cut(s) 300, 395
Bst4CI ACNGT 3 cut(s) 356, 685, 737
Bst6I CTCTTC 2 cut(s) 259, 399
BstACI GRCGYC 1 cut(s) 242
BstAPI GCANNNNNTGC 2 cut(s) 12, 225
BstC8I GCNNGC 4 cut(s) 230, 540, 544, 870
BstDEI CTNAG 3 cut(s) 519, 533, 727
BstDSI CCRYGG 2 cut(s) 300, 395
BstF5I GGATG 2 cut(s) 310, 911
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 5 cut(s) 130, 136, 625, 733, 856
BstMAI GTCTC 2 cut(s) 288, 362
BstMBI GATC 5 cut(s) 127, 133, 622, 730, 853
BstMWI GCNNNNNNNGC 3 cut(s) 12, 225, 515
BstV1I GCAGC 4 cut(s) 606, 722, 784, 859
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 853
BstYI RGATCY 1 cut(s) 853
BsuRI GGCC 2 cut(s) 36, 668
BtgI CCRYGG 2 cut(s) 300, 395
BtsCI GGATG 2 cut(s) 310, 911
BtsIMutI CAGTG 1 cut(s) 690
BveI ACCTGC 2 cut(s) 59, 917
Cac8I GCNNGC 4 cut(s) 230, 540, 544, 870
CciI TCATGA 3 cut(s) 124, 130, 144
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 2 cut(s) 48, 929
CseI GACGC 1 cut(s) 828
Csp6I GTAC 3 cut(s) 81, 759, 848
CviQI GTAC 3 cut(s) 81, 759, 848
DdeI CTNAG 3 cut(s) 519, 533, 727
DpnI GATC 5 cut(s) 129, 135, 624, 732, 855
DpnII GATC 5 cut(s) 127, 133, 622, 730, 853
DraIII CACNNNGTG 1 cut(s) 23
DrdI GACNNNNNNGTC 1 cut(s) 244
DseDI GACNNNNNNGTC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 666
Eam1104I CTCTTC 2 cut(s) 259, 399
EarI CTCTTC 2 cut(s) 259, 399
Ecl136II GAGCTC 1 cut(s) 862
Eco130I CCWWGG 2 cut(s) 300, 395
Eco24I GRGCYC 2 cut(s) 373, 864
Eco31I GGTCTC 1 cut(s) 362
Eco47I GGWCC 2 cut(s) 48, 929
Eco53kI GAGCTC 1 cut(s) 862
Eco88I CYCGRG 1 cut(s) 434
EcoICRI GAGCTC 1 cut(s) 862
EcoO109I RGGNCCY 2 cut(s) 48, 929
EcoRI GAATTC 1 cut(s) 820
EcoT14I CCWWGG 2 cut(s) 300, 395
EcoT38I GRGCYC 2 cut(s) 373, 864
ErhI CCWWGG 2 cut(s) 300, 395
FaqI GGGAC 1 cut(s) 915
FauNDI CATATG 1 cut(s) 362
FbaI TGATCA 2 cut(s) 127, 133
Fnu4HI GCNGC 4 cut(s) 595, 711, 773, 873
FokI GGATG 2 cut(s) 317, 898
FriOI GRGCYC 2 cut(s) 373, 864
Fsp4HI GCNGC 4 cut(s) 595, 711, 773, 873
FspBI CTAG 7 cut(s) 45, 75, 84, 471, 570, 857, 882
FspI TGCGCA 1 cut(s) 55
GlaI GCGC 1 cut(s) 55
GluI GCNGC 4 cut(s) 595, 711, 773, 873
GsaI CCCAGC 1 cut(s) 926
GsuI CTGGAG 1 cut(s) 478
HaeIII GGCC 2 cut(s) 36, 668
HapII CCGG 3 cut(s) 415, 779, 851
HgaI GACGC 1 cut(s) 828
HhaI GCGC 1 cut(s) 56
Hin1I GRCGYC 1 cut(s) 242
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HincII GTYRAC 1 cut(s) 798
HindII GTYRAC 1 cut(s) 798
HinfI GANTC 4 cut(s) 432, 511, 674, 803
HpaII CCGG 3 cut(s) 415, 779, 851
HphI GGTGA 1 cut(s) 464
Hpy166II GTNNAC 1 cut(s) 798
Hpy188I TCNGA 5 cut(s) 65, 325, 352, 431, 522
Hpy188III TCNNGA 5 cut(s) 125, 131, 145, 436, 857
Hpy8I GTNNAC 1 cut(s) 798
Hpy99I CGWCG 4 cut(s) 241, 244, 247, 567
HpyAV CCTTC 2 cut(s) 297, 379
HpyCH4III ACNGT 3 cut(s) 356, 685, 737
HpyCH4IV ACGT 4 cut(s) 242, 419, 565, 640
HpyCH4V TGCA 3 cut(s) 320, 710, 875
HpyF10VI GCNNNNNNNGC 3 cut(s) 12, 225, 515
HpyF3I CTNAG 3 cut(s) 519, 533, 727
HpySE526I ACGT 4 cut(s) 242, 419, 565, 640
Hsp92I GRCGYC 1 cut(s) 242
HspAI GCGC 1 cut(s) 54
Ksp22I TGATCA 2 cut(s) 127, 133
Kzo9I GATC 5 cut(s) 127, 133, 622, 730, 853
LmnI GCTCC 1 cut(s) 409
Lsp1109I GCAGC 4 cut(s) 606, 722, 784, 859
LweI GCATC 1 cut(s) 329
MaeI CTAG 7 cut(s) 45, 75, 84, 471, 570, 857, 882
MaeII ACGT 4 cut(s) 242, 419, 565, 640
MaeIII GTNAC 4 cut(s) 452, 481, 641, 958
MalI GATC 5 cut(s) 129, 135, 624, 732, 855
MboI GATC 5 cut(s) 127, 133, 622, 730, 853
MboII GAAGA 8 cut(s) 144, 217, 246, 252, 267, 394, 416, 819
MflI RGATCY 1 cut(s) 853
MhlI GDGCHC 4 cut(s) 17, 373, 414, 864
MlsI TGGCCA 1 cut(s) 668
MluCI AATT 8 cut(s) 58, 172, 181, 271, 475, 627, 820, 951
MluNI TGGCCA 1 cut(s) 668
MlyI GAGTC 2 cut(s) 426, 520
MmeI TCCRAC 2 cut(s) 73, 139
MnlI CCTC 9 cut(s) 59, 262, 343, 400, 455, 584, 699, 756, 944
Mox20I TGGCCA 1 cut(s) 668
MroXI GAANNNNTTC 1 cut(s) 212
MscI TGGCCA 1 cut(s) 668
MseI TTAA 1 cut(s) 939
MslI CAYNNNNRTG 2 cut(s) 129, 200
Msp20I TGGCCA 1 cut(s) 668
MspI CCGG 3 cut(s) 415, 779, 851
MwoI GCNNNNNNNGC 3 cut(s) 12, 225, 515
NcoI CCATGG 2 cut(s) 300, 395
NdeI CATATG 1 cut(s) 362
NdeII GATC 5 cut(s) 127, 133, 622, 730, 853
NlaIV GGNNCC 1 cut(s) 931
NmeAIII GCCGAG 2 cut(s) 392, 748
NmuCI GTSAC 4 cut(s) 452, 481, 641, 958
NsbI TGCGCA 1 cut(s) 55
PaeR7I CTCGAG 1 cut(s) 434
PagI TCATGA 3 cut(s) 124, 130, 144
PcsI WCGNNNNNNNCGW 3 cut(s) 242, 798, 836
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 2 cut(s) 674, 803
PflFI GACNNNGTC 2 cut(s) 241, 244
PkrI GCNGC 4 cut(s) 596, 712, 774, 874
PleI GAGTC 2 cut(s) 426, 519
PpsI GAGTC 2 cut(s) 426, 519
PpuMI RGGWCCY 2 cut(s) 48, 929
Psp124BI GAGCTC 1 cut(s) 864
Psp5II RGGWCCY 2 cut(s) 48, 929
PspFI CCCAGC 1 cut(s) 922
PspN4I GGNNCC 1 cut(s) 931
PspPI GGNCC 2 cut(s) 48, 929
PspPPI RGGWCCY 2 cut(s) 48, 929
PsuI RGATCY 1 cut(s) 853
PsyI GACNNNGTC 2 cut(s) 241, 244
RsaI GTAC 3 cut(s) 82, 760, 849
RsaNI GTAC 3 cut(s) 81, 759, 848
RseI CAYNNNNRTG 2 cut(s) 129, 200
SacI GAGCTC 1 cut(s) 864
SaqAI TTAA 1 cut(s) 939
SatI GCNGC 4 cut(s) 595, 711, 773, 873
Sau3AI GATC 5 cut(s) 127, 133, 622, 730, 853
Sau96I GGNCC 2 cut(s) 48, 929
ScaI AGTACT 2 cut(s) 82, 760
SchI GAGTC 2 cut(s) 426, 520
SduI GDGCHC 4 cut(s) 17, 373, 414, 864
SfaNI GCATC 1 cut(s) 329
Sfr274I CTCGAG 1 cut(s) 434
SinI GGWCC 2 cut(s) 48, 929
SlaI CTCGAG 1 cut(s) 434
SmiMI CAYNNNNRTG 2 cut(s) 129, 200
SmlI CTYRAG 2 cut(s) 434, 501
SmoI CTYRAG 2 cut(s) 434, 501
SpeI ACTAGT 3 cut(s) 74, 83, 881
Sse9I AATT 8 cut(s) 58, 172, 181, 271, 475, 627, 820, 951
SspI AATATT 2 cut(s) 313, 649
SspMI CTAG 7 cut(s) 45, 75, 84, 471, 570, 857, 882
SstI GAGCTC 1 cut(s) 864
StyI CCWWGG 2 cut(s) 300, 395
TaaI ACNGT 3 cut(s) 356, 685, 737
TaiI ACGT 4 cut(s) 245, 422, 568, 643
TaqI TCGA 2 cut(s) 435, 830
TaqII GACCGA 1 cut(s) 815
TasI AATT 8 cut(s) 58, 172, 181, 271, 475, 627, 820, 951
TatI WGTACW 2 cut(s) 80, 758
TfiI GAWTC 2 cut(s) 674, 803
Tru1I TTAA 1 cut(s) 939
Tru9I TTAA 1 cut(s) 939
TscAI CASTG 1 cut(s) 690
TseFI GTSAC 4 cut(s) 452, 481, 641, 958
TseI GCWGC 4 cut(s) 594, 710, 772, 872
Tsp45I GTSAC 4 cut(s) 452, 481, 641, 958
TspDTI ATGAA 9 cut(s) 110, 144, 179, 218, 246, 267, 321, 813, 827
TspGWI ACGGA 1 cut(s) 416
TspRI CASTG 1 cut(s) 690
Tth111I GACNNNGTC 2 cut(s) 241, 244
VpaK11BI GGWCC 2 cut(s) 48, 929
XapI RAATTY 2 cut(s) 58, 820
XbaI TCTAGA 1 cut(s) 856
XhoI CTCGAG 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 212
XspI CTAG 7 cut(s) 45, 75, 84, 471, 570, 857, 882
ZraI GACGTC 1 cut(s) 243
ZrmI AGTACT 2 cut(s) 82, 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.