MD02G1086100.v1.1

Prolyl oligopeptidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013157
Physical Location & Seq
Reverse (-)
6755952 .. 6759379
3428 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1086100.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGTGTCGTACGTGAGCGCGCTGCTGTACGGAGTGGGAGGCCTGGTGGTGGCCGGCATGACATTGCTCGTCGCTCTCCAGGAGCGCCTCGTCTACGTCCCCGTCTTGCCGGGTCTCACCAAGTCCTACCCGATTACCCCCGCCCGCCTCCGCCTCACATACGAGGACGTCTGGCTCCAGTCCTCCGACGGCGTTCGCCTCCACGCTTGGTTCATCAAGCTCTTCCCTCTCTGCCGAGGTCCAACCCTATTGTTTTTCCAAGAAAATGCTGGAAATATTGCTCATCGTCTTGAAATGGTCCGCATAATGTTACAACAACTGCAATGCAACGTGTTTATGCTTTCATATCGAGGTTATGGAGCAAGTGAAGGATATCCTTCTCAGCACGGGATCATAAAAGATGCTCAGGTTGCATTGGATCACCTATCGCAGAGGACTGACATTGACACATCTAGAATAGTTGTCTTTGGAAGGTCACTTGGGGGTGCAGTTGGAACTGTGGTTGCTAAAAACAACCCTGATAAGGTTGCTGCACTGATACTGGAAAACACTTTCACATCTATTTTGGACTTGGTTGGAGTTTTATTTCCTTTCTTGAAGTGGTTCATTGGAGGCAGTGGTTCAAAAGGTCCTAAAGTACTCAATTTTCTCGTACGTTCTCCTTGGAGTACAATTGATGTCATTGGTGAGGTTAAGCAGCCAATCCTCTTTCTTTCCGGCTTGCAAGATGAGATGATTCCTCCATCCCATTTGCAAATGCTGTATGCCAAAGCAGCTGCCCATAATAAGCGGTGTGTTTTTGTGGAATTTCCCACTGGCATGCATATGGATACTTGGCTTGCTGGTGGTGACGAGTATTGGAGAACGGTCCAGGAGTTTCTTCAACAACATGTTCCAGCGAAAAAAGATGATGGATCCTCTCACAGTGATAGCGCTGCAGCAATTGGAAGCTCGCTTTGA

Protein Analysis

320

Amino Acids

35.14

Weight (kDa)

7.77

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 103 - 195 8e-10 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 104 - 202 2.8e-09 alpha/beta hydrolase fold
Peptidase_S9 PF00326 132 - 297 8.3e-12 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20520
fragaria_vesca FvH4_1g08150 FvH4_1g08150
malus_domestica MD02G1086100.v1.1 MD15G1213300.v1.1
prunus_persica Prupe.7G203600_v2.0.a1 Prupe.7G203600_v2.0.a1
pyrus_communis pycom02g06930 pycom15g18970
rosa_chinensis RchiOBHm_Chr2g0094221
rosa_laevigata RLG00000016467
rosa_multiflora Rmu_sc0002193.1_g000008
rosa_roxburghii Rroxscaffold_2G00147360
rosa_rugosa Rorug02G0041900
rosa_samantha Rh2AG089700 Rh2BG089800 Rh2CG092300 Rh2DG088600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 171
AccI GTMKAC 1 cut(s) 93
AccII CGCG 1 cut(s) 20
AciI CCGC 5 cut(s) 141, 145, 151, 301, 790
AclWI GGATC 4 cut(s) 398, 426, 909, 922
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 1 cut(s) 806
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 5 cut(s) 11, 29, 639, 654, 670
AfeI AGCGCT 1 cut(s) 934
AfiI CCNNNNNNNGG 2 cut(s) 49, 523
AflIII ACRYGT 2 cut(s) 330, 889
AgsI TTSAA 4 cut(s) 293, 598, 624, 884
AjnI CCWGG 3 cut(s) 42, 78, 870
AluBI AGCT 3 cut(s) 220, 776, 951
AluI AGCT 3 cut(s) 220, 776, 951
Alw26I GTCTC 1 cut(s) 119
AlwI GGATC 4 cut(s) 398, 426, 909, 922
Aor51HI AGCGCT 1 cut(s) 934
AoxI GGCC 2 cut(s) 40, 51
ApeKI GCWGC 7 cut(s) 22, 530, 697, 773, 776, 935, 938
ApoI RAATTY 1 cut(s) 806
Asp700I GAANNNNTTC 1 cut(s) 602
AspLEI GCGC 4 cut(s) 20, 22, 87, 935
AspS9I GGNCC 4 cut(s) 239, 298, 629, 868
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 4 cut(s) 109, 413, 698, 860
AvaII GGWCC 4 cut(s) 239, 298, 629, 868
BamHI GGATCC 1 cut(s) 914
BbvI GCAGC 7 cut(s) 9, 517, 709, 763, 785, 922, 950
BccI CCATC 2 cut(s) 751, 905
BceAI ACGGC 1 cut(s) 205
BciT130I CCWGG 3 cut(s) 44, 80, 872
BciVI GTATCC 1 cut(s) 823
BcnI CCSGG 1 cut(s) 111
BcoDI GTCTC 1 cut(s) 119
BfaI CTAG 1 cut(s) 453
BfmI CTRYAG 1 cut(s) 936
BfoI RGCGCY 2 cut(s) 88, 936
BfuI GTATCC 1 cut(s) 823
BisI GCNGC 7 cut(s) 23, 531, 698, 774, 777, 936, 939
BlsI GCNGC 7 cut(s) 24, 532, 699, 775, 778, 937, 940
BmcAI AGTACT 1 cut(s) 639
Bme1390I CCNGG 4 cut(s) 44, 80, 111, 872
Bme18I GGWCC 4 cut(s) 239, 298, 629, 868
BmgT120I GGNCC 4 cut(s) 239, 298, 629, 868
BmiI GGNNCC 2 cut(s) 176, 916
BmrFI CCNGG 4 cut(s) 44, 80, 111, 872
BmsI GCATC 1 cut(s) 391
BpmI CTGGAG 2 cut(s) 62, 161
Bpu10I CCTNAGC 1 cut(s) 405
BpuMI CCSGG 1 cut(s) 111
BsaAI YACGTR 1 cut(s) 13
BsaHI GRCGYC 1 cut(s) 168
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 2 cut(s) 235, 662
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 523
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 3 cut(s) 178, 546, 820
Bse3DI GCAATG 2 cut(s) 62, 329
BseBI CCWGG 3 cut(s) 44, 80, 872
BseDI CCNNGG 2 cut(s) 235, 662
BseGI GGATG 1 cut(s) 743
BseLI CCNNNNNNNGG 2 cut(s) 49, 523
BseMI GCAATG 2 cut(s) 62, 329
BseMII CTCAG 2 cut(s) 395, 419
BseNI ACTGG 3 cut(s) 178, 546, 820
BsePI GCGCGC 1 cut(s) 18
BseXI GCAGC 7 cut(s) 9, 517, 709, 763, 785, 922, 950
BsgI GTGCAG 2 cut(s) 507, 516
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 2 cut(s) 42, 53
BsiSI CCGG 3 cut(s) 54, 110, 717
BsiWI CGTACG 2 cut(s) 9, 652
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 2 cut(s) 49, 523
BsmAI GTCTC 1 cut(s) 119
BsmFI GGGAC 1 cut(s) 83
BsnI GGCC 2 cut(s) 42, 53
Bso31I GGTCTC 1 cut(s) 119
Bsp143I GATC 3 cut(s) 390, 418, 914
BspACI CCGC 5 cut(s) 141, 145, 151, 301, 790
BspANI GGCC 2 cut(s) 42, 53
BspCNI CTCAG 2 cut(s) 394, 418
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 2 cut(s) 176, 916
BspMAI CTGCAG 1 cut(s) 940
BspPI GGATC 4 cut(s) 398, 426, 909, 922
BspQI GCTCTTC 1 cut(s) 227
BspTNI GGTCTC 1 cut(s) 119
BsrDI GCAATG 2 cut(s) 62, 329
BsrFI RCCGGY 1 cut(s) 53
BsrI ACTGG 3 cut(s) 178, 546, 820
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 2 cut(s) 235, 662
BssHII GCGCGC 1 cut(s) 18
BssMI GATC 3 cut(s) 390, 418, 914
BssNI GRCGYC 1 cut(s) 168
BssT1I CCWWGG 1 cut(s) 662
Bst2UI CCWGG 3 cut(s) 44, 80, 872
Bst4CI ACNGT 3 cut(s) 499, 868, 926
Bst6I CTCTTC 1 cut(s) 227
BstACI GRCGYC 1 cut(s) 168
BstBAI YACGTR 1 cut(s) 13
BstC8I GCNNGC 7 cut(s) 20, 55, 145, 722, 821, 840, 953
BstDEI CTNAG 2 cut(s) 381, 405
BstF5I GGATG 1 cut(s) 743
BstFNI CGCG 1 cut(s) 20
BstH2I RGCGCY 2 cut(s) 88, 936
BstHHI GCGC 4 cut(s) 20, 22, 87, 935
BstKTI GATC 3 cut(s) 393, 421, 917
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 3 cut(s) 390, 418, 914
BstMWI GCNNNNNNNGC 2 cut(s) 410, 773
BstNI CCWGG 3 cut(s) 44, 80, 872
BstNSI RCATGY 2 cut(s) 823, 893
BstSCI CCNGG 4 cut(s) 42, 78, 109, 870
BstSFI CTRYAG 1 cut(s) 936
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 7 cut(s) 9, 517, 709, 763, 785, 922, 950
BstX2I RGATCY 1 cut(s) 914
BstYI RGATCY 1 cut(s) 914
BsuI GTATCC 1 cut(s) 823
BsuRI GGCC 2 cut(s) 42, 53
BtsCI GGATG 1 cut(s) 743
BtsI GCAGTG 1 cut(s) 622
BtsIMutI CAGTG 4 cut(s) 533, 622, 813, 931
Cac8I GCNNGC 7 cut(s) 20, 55, 145, 722, 821, 840, 953
CfoI GCGC 4 cut(s) 20, 22, 87, 935
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 4 cut(s) 239, 298, 629, 868
Csp6I GTAC 5 cut(s) 10, 28, 638, 653, 669
CviAII CATG 3 cut(s) 58, 820, 890
CviJI RGCY 9 cut(s) 42, 53, 175, 220, 700, 720, 776, 838, 951
CviKI_1 RGCY 9 cut(s) 42, 53, 175, 220, 700, 720, 776, 838, 951
CviQI GTAC 5 cut(s) 10, 28, 638, 653, 669
DdeI CTNAG 2 cut(s) 381, 405
DpnI GATC 3 cut(s) 392, 420, 916
DpnII GATC 3 cut(s) 390, 418, 914
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
EciI GGCGGA 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 662
Eco147I AGGCCT 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 119
Eco32I GATATC 1 cut(s) 374
Eco47I GGWCC 4 cut(s) 239, 298, 629, 868
Eco47III AGCGCT 1 cut(s) 934
EcoO109I RGGNCCY 1 cut(s) 629
EcoRII CCWGG 3 cut(s) 42, 78, 870
EcoRV GATATC 1 cut(s) 374
EcoT14I CCWWGG 1 cut(s) 662
EcoT22I ATGCAT 1 cut(s) 825
ErhI CCWWGG 1 cut(s) 662
FaeI CATG 3 cut(s) 61, 823, 893
FaqI GGGAC 1 cut(s) 83
FatI CATG 3 cut(s) 57, 819, 889
FauI CCCGC 2 cut(s) 148, 152
FauNDI CATATG 1 cut(s) 825
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 7 cut(s) 23, 531, 698, 774, 777, 936, 939
FokI GGATG 1 cut(s) 730
Fsp4HI GCNGC 7 cut(s) 23, 531, 698, 774, 777, 936, 939
FspBI CTAG 1 cut(s) 453
GlaI GCGC 4 cut(s) 19, 21, 86, 934
GluI GCNGC 7 cut(s) 23, 531, 698, 774, 777, 936, 939
GsuI CTGGAG 2 cut(s) 62, 161
HaeII RGCGCY 2 cut(s) 88, 936
HaeIII GGCC 2 cut(s) 42, 53
HapII CCGG 3 cut(s) 54, 110, 717
HhaI GCGC 4 cut(s) 20, 22, 87, 935
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 3 cut(s) 61, 823, 893
Hin6I GCGC 4 cut(s) 18, 20, 85, 933
HinP1I GCGC 4 cut(s) 18, 20, 85, 933
HinfI GANTC 1 cut(s) 736
HpaII CCGG 3 cut(s) 54, 110, 717
HphI GGTGA 4 cut(s) 109, 413, 698, 860
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 1 cut(s) 187
Hpy188III TCNNGA 3 cut(s) 290, 453, 595
Hpy8I GTNNAC 1 cut(s) 94
Hpy99I CGWCG 2 cut(s) 74, 191
HpyAV CCTTC 3 cut(s) 362, 387, 465
HpyCH4III ACNGT 3 cut(s) 499, 868, 926
HpyCH4IV ACGT 5 cut(s) 12, 96, 168, 330, 655
HpyCH4V TGCA 9 cut(s) 322, 327, 413, 488, 533, 724, 754, 823, 938
HpyF10VI GCNNNNNNNGC 2 cut(s) 410, 773
HpyF3I CTNAG 2 cut(s) 381, 405
HpySE526I ACGT 5 cut(s) 12, 96, 168, 330, 655
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 3 cut(s) 61, 823, 893
HspAI GCGC 4 cut(s) 18, 20, 85, 933
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Kzo9I GATC 3 cut(s) 390, 418, 914
LguI GCTCTTC 1 cut(s) 227
LmnI GCTCC 3 cut(s) 82, 180, 359
Lsp1109I GCAGC 7 cut(s) 9, 517, 709, 763, 785, 922, 950
LweI GCATC 1 cut(s) 391
MaeI CTAG 1 cut(s) 453
MaeII ACGT 5 cut(s) 12, 96, 168, 330, 655
MaeIII GTNAC 3 cut(s) 309, 474, 848
MalI GATC 3 cut(s) 392, 420, 916
MboI GATC 3 cut(s) 390, 418, 914
MboII GAAGA 2 cut(s) 214, 872
MfeI CAATTG 2 cut(s) 672, 942
MflI RGATCY 1 cut(s) 914
MluCI AATT 4 cut(s) 643, 672, 806, 942
MmeI TCCRAC 4 cut(s) 210, 266, 472, 556
Mph1103I ATGCAT 1 cut(s) 825
MroNI GCCGGC 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 602
MseI TTAA 1 cut(s) 693
MslI CAYNNNNRTG 2 cut(s) 818, 824
MspA1I CMGCKG 1 cut(s) 776
MspI CCGG 3 cut(s) 54, 110, 717
MspR9I CCNGG 4 cut(s) 44, 80, 111, 872
MunI CAATTG 2 cut(s) 672, 942
MvaI CCWGG 3 cut(s) 44, 80, 872
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 2 cut(s) 410, 773
NaeI GCCGGC 1 cut(s) 55
NciI CCSGG 1 cut(s) 111
NdeI CATATG 1 cut(s) 825
NdeII GATC 3 cut(s) 390, 418, 914
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 3 cut(s) 61, 823, 893
NlaIV GGNNCC 2 cut(s) 176, 916
NmeAIII GCCGAG 1 cut(s) 260
NmuCI GTSAC 2 cut(s) 474, 848
NsiI ATGCAT 1 cut(s) 825
NspI RCATGY 2 cut(s) 823, 893
PaeI GCATGC 1 cut(s) 823
PauI GCGCGC 1 cut(s) 18
PceI AGGCCT 1 cut(s) 42
PciI ACATGT 1 cut(s) 889
PciSI GCTCTTC 1 cut(s) 227
PdiI GCCGGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 602
PfeI GAWTC 1 cut(s) 736
Pfl23II CGTACG 2 cut(s) 9, 652
PfoI TCCNGGA 2 cut(s) 78, 870
PkrI GCNGC 7 cut(s) 24, 532, 699, 775, 778, 937, 940
Ppu21I YACGTR 1 cut(s) 13
PpuMI RGGWCCY 1 cut(s) 629
PscI ACATGT 1 cut(s) 889
Psp5II RGGWCCY 1 cut(s) 629
Psp6I CCWGG 3 cut(s) 42, 78, 870
PspGI CCWGG 3 cut(s) 42, 78, 870
PspLI CGTACG 2 cut(s) 9, 652
PspN4I GGNNCC 2 cut(s) 176, 916
PspPI GGNCC 4 cut(s) 239, 298, 629, 868
PspPPI RGGWCCY 1 cut(s) 629
PstI CTGCAG 1 cut(s) 940
PsuI RGATCY 1 cut(s) 914
PteI GCGCGC 1 cut(s) 18
PvuII CAGCTG 1 cut(s) 776
RsaI GTAC 5 cut(s) 11, 29, 639, 654, 670
RsaNI GTAC 5 cut(s) 10, 28, 638, 653, 669
RseI CAYNNNNRTG 2 cut(s) 818, 824
SapI GCTCTTC 1 cut(s) 227
SaqAI TTAA 1 cut(s) 693
SatI GCNGC 7 cut(s) 23, 531, 698, 774, 777, 936, 939
Sau3AI GATC 3 cut(s) 390, 418, 914
Sau96I GGNCC 4 cut(s) 239, 298, 629, 868
ScaI AGTACT 1 cut(s) 639
ScrFI CCNGG 4 cut(s) 44, 80, 111, 872
SfaNI GCATC 1 cut(s) 391
SfcI CTRYAG 1 cut(s) 936
SinI GGWCC 4 cut(s) 239, 298, 629, 868
SmiMI CAYNNNNRTG 2 cut(s) 818, 824
SphI GCATGC 1 cut(s) 823
Sse9I AATT 4 cut(s) 643, 672, 806, 942
SseBI AGGCCT 1 cut(s) 42
SsiI CCGC 5 cut(s) 141, 145, 151, 301, 790
SspI AATATT 1 cut(s) 277
SspMI CTAG 1 cut(s) 453
StuI AGGCCT 1 cut(s) 42
StyD4I CCNGG 4 cut(s) 42, 78, 109, 870
StyI CCWWGG 1 cut(s) 662
TaaI ACNGT 3 cut(s) 499, 868, 926
TaiI ACGT 5 cut(s) 15, 99, 171, 333, 658
TaqI TCGA 1 cut(s) 349
TasI AATT 4 cut(s) 643, 672, 806, 942
TatI WGTACW 2 cut(s) 637, 668
TfiI GAWTC 1 cut(s) 736
Tru1I TTAA 1 cut(s) 693
Tru9I TTAA 1 cut(s) 693
TscAI CASTG 4 cut(s) 540, 622, 820, 931
TseFI GTSAC 2 cut(s) 474, 848
TseI GCWGC 7 cut(s) 22, 530, 697, 773, 776, 935, 938
Tsp45I GTSAC 2 cut(s) 474, 848
TspDTI ATGAA 3 cut(s) 202, 333, 595
TspGWI ACGGA 1 cut(s) 45
TspRI CASTG 4 cut(s) 540, 622, 820, 931
VpaK11BI GGWCC 4 cut(s) 239, 298, 629, 868
XapI RAATTY 1 cut(s) 806
XbaI TCTAGA 1 cut(s) 452
XceI RCATGY 2 cut(s) 823, 893
XcmI CCANNNNNNNNNTGG 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 93
XmnI GAANNNNTTC 1 cut(s) 602
XspI CTAG 1 cut(s) 453
ZraI GACGTC 1 cut(s) 169
ZrmI AGTACT 1 cut(s) 639
Zsp2I ATGCAT 1 cut(s) 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.