MD02G1095200.v1.1

Transmembrane protein 184 homolog DDB_G0279555

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012223
Physical Location & Seq
Reverse (-)
7519129 .. 7522281
3153 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1095200.v1.1.491

Sequence Viewer

Length: 1305 bp
ATGAATTCTTTGGGAGCGGAACATGTTCGAGTAATGGCATCAGATTATAATTCAGTCCATGGAAACATATACGCAGATCTCTACAAACCAGCTGTTATCATTGGAGCCTGTTTTACAGCTGTTGCACTCGCCCTCTCCATTTTTCTCATTTTCCAACATCTCAGATCGTACAATAAACCCAGTGAACAAAAATGGCTCGTTGCCGTAGTCTTCATGGTACCTGTATATGCTACTGAGTCAATTTTATCCTTGTGGAACCCGAACCTTTCTCTTGCCTGTGACATTTTAAGAAACTGTTATGAAGCATTTGCCTTGTATTCTTTCGGAAGCTACTTAGTTGCCTGTCTCGGAGGTGAAAGAAAGGTCATAGAACTACTTGAAAACCAATCGGAAAAACTGCTCAACAGACCACTGATAGAAGGAGTGGCAGACGAGGATCACACCGTGCCACATTGGTCCCTTCGAAACTTCTTCTTACGACCATCTGTTCTTGGGAAACATTTGCTCAATATAGAAAAATTTGGCCTTGTACAATATATGATTCTGAAGACAGTTTGTGCATTCTTAGCTTTAGTGTTGGAGCTCTTTGGTGTGTATGGAGACGGAGAATTCAAGTGGTATTACGGGTATCCATACATGGCTGTTGTACTGAACTTCAGCCAGATGTGGGCATTGTATTGCCTTGTGCAATTCTACAATGTAACACACGAAAGACTTGAACCTATTAGGCCGCTCGCAAAGTTCATCAGCTTTAAAGCTATTGTGTTCGCCACTTGGTGGCAAGGTGTGGGCATTGCGCTATTATGCGCATTTGGTGTTCTTCCTAAGGACGTTAAACTACAAAGCGGCTTGCAGAATTTCCTGATTTGTATTGAAATGGCAATTGCAGCCGTTGCGCATATGTTTGTCTTTTCAACTGAACCATACCATTACATTACAGCATACGAGTATGGGAAGGTCACCACTGAAACAACCAAGGCAGAAGTGAAGTTGGAGGAAGGTGACGCCCCAGCCAGGCTCGAAAAAACGGAGACCAAGGTCGAAGCGCCTGGAACAAGTATCACTGAGAGTGTTCAGGACATTGTTGTTGACGGCGGTCAGCGTGTTGTCAAGGATGTTGTGTTGACCATAAATCAAGCAATAGGGCCAGTTGAGAAGGGAGTCGAGAAGGGCGTGACGAAGATCCAGGAGACGCTCCATCACAGAACAGTGGACTCGGAATCGGGAGAACACGAAGATAAGTCAGAAGTAGAAGTTGAGGAACAATTCGGAAATACTGTCAGAGAAACTCATATCGACCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0016020 GO:0016021 GO:0031224 GO:0044425
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Solute_trans_a

Protein Analysis

435

Amino Acids

48.81

Weight (kDa)

5.37

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Solute_trans_a PF03619 32 - 309 9.7e-95 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23070
fragaria_vesca FvH4_1g08970 FvH4_1g08970 FvH4_1g08970 FvH4_1g08970 FvH4_1g08970 FvH4_1g08970
malus_domestica MD02G1095200.v1.1
prunus_persica Prupe.7G196000_v2.0.a1
pyrus_communis pycom02g07520
rosa_chinensis RchiOBHm_Chr2g0095421
rosa_laevigata RLG00000016564
rosa_multiflora Rmu_co8230917.1_g000001 Rmu_sc0010847.1_g000005
rosa_samantha Rh2AG098900 Rh2BG100100 Rh2CG102100 Rh2DG099700
rosa_wichuraiana Rw2G007470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 48
Acc16I TGCGCA 2 cut(s) 808, 897
Acc65I GGTACC 1 cut(s) 217
AccB1I GGYRCC 1 cut(s) 217
AccB7I CCANNNNNTGG 1 cut(s) 777
AccBSI CCGCTC 2 cut(s) 17, 733
AciI CCGC 4 cut(s) 17, 731, 846, 1095
AclWI GGATC 2 cut(s) 444, 1177
AcsI RAATTY 4 cut(s) 4, 518, 608, 856
AcuI CTGAAG 2 cut(s) 566, 640
AcyI GRCGYC 1 cut(s) 1005
AdeI CACNNNGTG 2 cut(s) 445, 777
AfaI GTAC 4 cut(s) 170, 219, 531, 648
AfiI CCNNNNNNNGG 3 cut(s) 667, 777, 1014
AflIII ACRYGT 1 cut(s) 22
AgsI TTSAA 5 cut(s) 380, 613, 719, 875, 915
AjnI CCWGG 3 cut(s) 1013, 1048, 1185
AluBI AGCT 7 cut(s) 92, 119, 330, 569, 583, 750, 758
AluI AGCT 7 cut(s) 92, 119, 330, 569, 583, 750, 758
Alw21I GWGCWC 1 cut(s) 585
Alw26I GTCTC 4 cut(s) 350, 594, 1025, 1184
AlwI GGATC 2 cut(s) 444, 1177
AoxI GGCC 3 cut(s) 523, 728, 1145
ApeKI GCWGC 1 cut(s) 887
ApoI RAATTY 4 cut(s) 4, 518, 608, 856
Asp700I GAANNNNTTC 1 cut(s) 24
Asp718I GGTACC 1 cut(s) 217
AspLEI GCGC 4 cut(s) 799, 809, 898, 1048
AspS9I GGNCC 2 cut(s) 456, 1145
AsuHPI GGTGA 3 cut(s) 365, 952, 1013
AsuII TTCGAA 1 cut(s) 463
AvaII GGWCC 1 cut(s) 456
AxyI CCTNAGG 1 cut(s) 825
BanI GGYRCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 585
BbsI GAAGAC 2 cut(s) 202, 554
Bbv12I GWGCWC 1 cut(s) 585
BbvI GCAGC 1 cut(s) 899
BccI CCATC 2 cut(s) 490, 1206
BceAI ACGGC 3 cut(s) 188, 875, 1108
BciT130I CCWGG 3 cut(s) 1015, 1050, 1187
BciVI GTATCC 1 cut(s) 639
BcoDI GTCTC 4 cut(s) 350, 594, 1025, 1184
BfmI CTRYAG 1 cut(s) 1301
BfoI RGCGCY 1 cut(s) 1049
BfuI GTATCC 1 cut(s) 639
BglII AGATCT 1 cut(s) 76
BisI GCNGC 3 cut(s) 731, 847, 888
BlsI GCNGC 3 cut(s) 732, 848, 889
Bme1390I CCNGG 3 cut(s) 1015, 1050, 1187
Bme18I GGWCC 1 cut(s) 456
BmgT120I GGNCC 2 cut(s) 456, 1145
BmiI GGNNCC 4 cut(s) 106, 219, 257, 458
BmrFI CCNGG 3 cut(s) 1015, 1050, 1187
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 47
BmuI ACTGGG 1 cut(s) 174
BoxI GACNNNNGTC 2 cut(s) 1037, 1095
BpiI GAAGAC 2 cut(s) 202, 554
Bpu14I TTCGAA 1 cut(s) 463
BsaHI GRCGYC 1 cut(s) 1005
BsaI GGTCTC 1 cut(s) 1025
BsaJI CCNNGG 3 cut(s) 58, 975, 1035
Bsc4I CCNNNNNNNGG 3 cut(s) 667, 777, 1014
Bse1I ACTGG 2 cut(s) 180, 1148
Bse21I CCTNAGG 1 cut(s) 825
Bse3DI GCAATG 1 cut(s) 792
BseBI CCWGG 3 cut(s) 1015, 1050, 1187
BseDI CCNNGG 3 cut(s) 58, 975, 1035
BseGI GGATG 1 cut(s) 1120
BseLI CCNNNNNNNGG 3 cut(s) 667, 777, 1014
BseMI GCAATG 1 cut(s) 792
BseMII CTCAG 3 cut(s) 175, 225, 1056
BseNI ACTGG 2 cut(s) 180, 1148
BseXI GCAGC 1 cut(s) 899
BseYI CCCAGC 1 cut(s) 1009
BshFI GGCC 3 cut(s) 525, 730, 1147
BshNI GGYRCC 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 585
BslFI GGGAC 1 cut(s) 442
BslI CCNNNNNNNGG 3 cut(s) 667, 777, 1014
BsmAI GTCTC 4 cut(s) 350, 594, 1025, 1184
BsmBI CGTCTC 2 cut(s) 594, 1184
BsmFI GGGAC 1 cut(s) 442
BsmI GAATGC 1 cut(s) 560
BsnI GGCC 3 cut(s) 525, 730, 1147
Bso31I GGTCTC 1 cut(s) 1025
Bsp119I TTCGAA 1 cut(s) 463
Bsp1286I GDGCHC 1 cut(s) 585
Bsp1407I TGTACA 1 cut(s) 529
Bsp143I GATC 4 cut(s) 76, 164, 436, 1182
Bsp19I CCATGG 1 cut(s) 58
BspACI CCGC 4 cut(s) 17, 731, 846, 1095
BspANI GGCC 3 cut(s) 525, 730, 1147
BspCNI CTCAG 3 cut(s) 174, 226, 1057
BspLI GGNNCC 4 cut(s) 106, 219, 257, 458
BspPI GGATC 2 cut(s) 444, 1177
BspT104I TTCGAA 1 cut(s) 463
BspT107I GGYRCC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 1025
BsrBI CCGCTC 2 cut(s) 17, 733
BsrDI GCAATG 1 cut(s) 792
BsrGI TGTACA 1 cut(s) 529
BsrI ACTGG 2 cut(s) 180, 1148
BssECI CCNNGG 3 cut(s) 58, 975, 1035
BssMI GATC 4 cut(s) 76, 164, 436, 1182
BssNI GRCGYC 1 cut(s) 1005
BssT1I CCWWGG 3 cut(s) 58, 975, 1035
Bst2UI CCWGG 3 cut(s) 1015, 1050, 1187
Bst4CI ACNGT 5 cut(s) 296, 445, 553, 1210, 1279
BstACI GRCGYC 1 cut(s) 1005
BstAPI GCANNNNNTGC 1 cut(s) 893
BstAUI TGTACA 1 cut(s) 529
BstBI TTCGAA 1 cut(s) 463
BstC8I GCNNGC 2 cut(s) 735, 851
BstDEI CTNAG 6 cut(s) 161, 234, 334, 565, 825, 1065
BstDSI CCRYGG 1 cut(s) 58
BstEII GGTNACC 1 cut(s) 958
BstF5I GGATG 1 cut(s) 1120
BstH2I RGCGCY 1 cut(s) 1049
BstHHI GCGC 4 cut(s) 799, 809, 898, 1048
BstKTI GATC 4 cut(s) 79, 167, 439, 1185
BstMAI GTCTC 4 cut(s) 350, 594, 1025, 1184
BstMBI GATC 4 cut(s) 76, 164, 436, 1182
BstMWI GCNNNNNNNGC 3 cut(s) 566, 887, 893
BstNI CCWGG 3 cut(s) 1015, 1050, 1187
BstNSI RCATGY 1 cut(s) 26
BstPAI GACNNNNGTC 2 cut(s) 1037, 1095
BstPI GGTNACC 1 cut(s) 958
BstSCI CCNGG 3 cut(s) 1013, 1048, 1185
BstSFI CTRYAG 1 cut(s) 1301
BstV1I GCAGC 1 cut(s) 899
BstV2I GAAGAC 2 cut(s) 202, 554
BstX2I RGATCY 2 cut(s) 76, 1182
BstYI RGATCY 2 cut(s) 76, 1182
Bsu36I CCTNAGG 1 cut(s) 825
BsuI GTATCC 1 cut(s) 639
BsuRI GGCC 3 cut(s) 525, 730, 1147
BtgI CCRYGG 1 cut(s) 58
BtsCI GGATG 1 cut(s) 1120
BtsIMutI CAGTG 5 cut(s) 187, 410, 963, 1062, 1215
Cac8I GCNNGC 2 cut(s) 735, 851
CfoI GCGC 4 cut(s) 799, 809, 898, 1048
Cfr13I GGNCC 2 cut(s) 456, 1145
CseI GACGC 2 cut(s) 1013, 1201
Csp6I GTAC 4 cut(s) 169, 218, 530, 647
CviAII CATG 4 cut(s) 23, 59, 214, 637
CviQI GTAC 4 cut(s) 169, 218, 530, 647
DdeI CTNAG 6 cut(s) 161, 234, 334, 565, 825, 1065
DpnI GATC 4 cut(s) 78, 166, 438, 1184
DpnII GATC 4 cut(s) 76, 164, 436, 1182
DraI TTTAAA 1 cut(s) 754
DraIII CACNNNGTG 2 cut(s) 445, 777
Ecl136II GAGCTC 1 cut(s) 583
Eco130I CCWWGG 3 cut(s) 58, 975, 1035
Eco24I GRGCYC 1 cut(s) 585
Eco31I GGTCTC 1 cut(s) 1025
Eco47I GGWCC 1 cut(s) 456
Eco53kI GAGCTC 1 cut(s) 583
Eco57I CTGAAG 2 cut(s) 566, 640
Eco81I CCTNAGG 1 cut(s) 825
Eco91I GGTNACC 1 cut(s) 958
EcoICRI GAGCTC 1 cut(s) 583
EcoO65I GGTNACC 1 cut(s) 958
EcoRI GAATTC 2 cut(s) 4, 608
EcoRII CCWGG 3 cut(s) 1013, 1048, 1185
EcoT14I CCWWGG 3 cut(s) 58, 975, 1035
EcoT38I GRGCYC 1 cut(s) 585
ErhI CCWWGG 3 cut(s) 58, 975, 1035
Esp3I CGTCTC 2 cut(s) 594, 1184
FaeI CATG 4 cut(s) 26, 62, 217, 640
FaqI GGGAC 1 cut(s) 442
FatI CATG 4 cut(s) 22, 58, 213, 636
FauNDI CATATG 1 cut(s) 900
Fnu4HI GCNGC 3 cut(s) 731, 847, 888
FokI GGATG 1 cut(s) 1127
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 3 cut(s) 731, 847, 888
FspAI RTGCGCAY 1 cut(s) 808
FspI TGCGCA 2 cut(s) 808, 897
GlaI GCGC 4 cut(s) 798, 808, 897, 1047
GluI GCNGC 3 cut(s) 731, 847, 888
GsaI CCCAGC 1 cut(s) 1013
HaeII RGCGCY 1 cut(s) 1049
HaeIII GGCC 3 cut(s) 525, 730, 1147
HgaI GACGC 2 cut(s) 1013, 1201
HhaI GCGC 4 cut(s) 799, 809, 898, 1048
Hin1I GRCGYC 1 cut(s) 1005
Hin1II CATG 4 cut(s) 26, 62, 217, 640
Hin6I GCGC 4 cut(s) 797, 807, 896, 1046
HinP1I GCGC 4 cut(s) 797, 807, 896, 1046
HincII GTYRAC 2 cut(s) 1090, 1125
HindII GTYRAC 2 cut(s) 1090, 1125
HinfI GANTC 5 cut(s) 236, 541, 1161, 1214, 1220
HphI GGTGA 3 cut(s) 365, 952, 1013
Hpy166II GTNNAC 4 cut(s) 185, 1090, 1125, 1213
Hpy188III TCNNGA 4 cut(s) 862, 1076, 1165, 1224
Hpy8I GTNNAC 4 cut(s) 185, 1090, 1125, 1213
HpyAV CCTTC 6 cut(s) 413, 470, 949, 992, 1150, 1162
HpyCH4III ACNGT 5 cut(s) 296, 445, 553, 1210, 1279
HpyCH4IV ACGT 1 cut(s) 831
HpyCH4V TGCA 5 cut(s) 125, 560, 688, 853, 887
HpyF10VI GCNNNNNNNGC 3 cut(s) 566, 887, 893
HpyF3I CTNAG 6 cut(s) 161, 234, 334, 565, 825, 1065
HpySE526I ACGT 1 cut(s) 831
Hsp92I GRCGYC 1 cut(s) 1005
Hsp92II CATG 4 cut(s) 26, 62, 217, 640
HspAI GCGC 4 cut(s) 797, 807, 896, 1046
KpnI GGTACC 1 cut(s) 221
Kzo9I GATC 4 cut(s) 76, 164, 436, 1182
LmnI GCTCC 4 cut(s) 14, 104, 580, 1200
Lsp1109I GCAGC 1 cut(s) 899
LweI GCATC 1 cut(s) 47
MaeII ACGT 1 cut(s) 831
MaeIII GTNAC 5 cut(s) 278, 700, 958, 1001, 1174
MalI GATC 4 cut(s) 78, 166, 438, 1184
MbiI CCGCTC 2 cut(s) 17, 733
MboI GATC 4 cut(s) 76, 164, 436, 1182
MboII GAAGA 6 cut(s) 202, 463, 559, 812, 1192, 1247
MfeI CAATTG 1 cut(s) 882
MflI RGATCY 2 cut(s) 76, 1182
MhlI GDGCHC 1 cut(s) 585
MluCI AATT 9 cut(s) 4, 49, 240, 518, 608, 689, 856, 882, 1265
MlyI GAGTC 3 cut(s) 245, 1170, 1208
MmeI TCCRAC 3 cut(s) 178, 558, 972
MnlI CCTC 5 cut(s) 143, 344, 427, 988, 1252
MroXI GAANNNNTTC 1 cut(s) 24
MseI TTAA 3 cut(s) 287, 753, 834
MspA1I CMGCKG 2 cut(s) 92, 119
MspR9I CCNGG 3 cut(s) 1015, 1050, 1187
MunI CAATTG 1 cut(s) 882
Mva1269I GAATGC 1 cut(s) 560
MvaI CCWGG 3 cut(s) 1015, 1050, 1187
MwoI GCNNNNNNNGC 3 cut(s) 566, 887, 893
NcoI CCATGG 1 cut(s) 58
NdeI CATATG 1 cut(s) 900
NdeII GATC 4 cut(s) 76, 164, 436, 1182
NlaIII CATG 4 cut(s) 26, 62, 217, 640
NlaIV GGNNCC 4 cut(s) 106, 219, 257, 458
NmuCI GTSAC 4 cut(s) 278, 958, 1001, 1174
NsbI TGCGCA 2 cut(s) 808, 897
NspI RCATGY 1 cut(s) 26
NspV TTCGAA 1 cut(s) 463
PciI ACATGT 1 cut(s) 22
PcsI WCGNNNNNNNCGW 1 cut(s) 1170
PctI GAATGC 1 cut(s) 560
PdmI GAANNNNTTC 1 cut(s) 24
PfeI GAWTC 2 cut(s) 541, 1220
PflMI CCANNNNNTGG 1 cut(s) 777
PfoI TCCNGGA 1 cut(s) 1185
PkrI GCNGC 3 cut(s) 732, 848, 889
PleI GAGTC 3 cut(s) 244, 1169, 1208
PpsI GAGTC 3 cut(s) 244, 1169, 1208
PscI ACATGT 1 cut(s) 22
PshAI GACNNNNGTC 2 cut(s) 1037, 1095
PsiI TTATAA 1 cut(s) 48
Psp124BI GAGCTC 1 cut(s) 585
Psp6I CCWGG 3 cut(s) 1013, 1048, 1185
PspEI GGTNACC 1 cut(s) 958
PspFI CCCAGC 1 cut(s) 1009
PspGI CCWGG 3 cut(s) 1013, 1048, 1185
PspN4I GGNNCC 4 cut(s) 106, 219, 257, 458
PspPI GGNCC 2 cut(s) 456, 1145
PsuI RGATCY 2 cut(s) 76, 1182
PvuII CAGCTG 2 cut(s) 92, 119
RsaI GTAC 4 cut(s) 170, 219, 531, 648
RsaNI GTAC 4 cut(s) 169, 218, 530, 647
SacI GAGCTC 1 cut(s) 585
SaqAI TTAA 3 cut(s) 287, 753, 834
SatI GCNGC 3 cut(s) 731, 847, 888
Sau3AI GATC 4 cut(s) 76, 164, 436, 1182
Sau96I GGNCC 2 cut(s) 456, 1145
SchI GAGTC 3 cut(s) 245, 1170, 1208
ScrFI CCNGG 3 cut(s) 1015, 1050, 1187
SduI GDGCHC 1 cut(s) 585
SfaNI GCATC 1 cut(s) 47
SfcI CTRYAG 1 cut(s) 1301
SfuI TTCGAA 1 cut(s) 463
SinI GGWCC 1 cut(s) 456
Sse9I AATT 9 cut(s) 4, 49, 240, 518, 608, 689, 856, 882, 1265
SsiI CCGC 4 cut(s) 17, 731, 846, 1095
SstI GAGCTC 1 cut(s) 585
StyD4I CCNGG 3 cut(s) 1013, 1048, 1185
StyI CCWWGG 3 cut(s) 58, 975, 1035
TaaI ACNGT 5 cut(s) 296, 445, 553, 1210, 1279
TaiI ACGT 1 cut(s) 834
TaqI TCGA 6 cut(s) 28, 463, 1020, 1041, 1164, 1296
TasI AATT 9 cut(s) 4, 49, 240, 518, 608, 689, 856, 882, 1265
TatI WGTACW 2 cut(s) 529, 646
TauI GCSGC 2 cut(s) 733, 849
TfiI GAWTC 2 cut(s) 541, 1220
Tru1I TTAA 3 cut(s) 287, 753, 834
Tru9I TTAA 3 cut(s) 287, 753, 834
TscAI CASTG 5 cut(s) 187, 417, 970, 1069, 1215
TseFI GTSAC 4 cut(s) 278, 958, 1001, 1174
TseI GCWGC 1 cut(s) 887
Tsp45I GTSAC 4 cut(s) 278, 958, 1001, 1174
TspDTI ATGAA 4 cut(s) 17, 202, 315, 733
TspGWI ACGGA 2 cut(s) 618, 1043
TspRI CASTG 5 cut(s) 187, 417, 970, 1069, 1215
Van91I CCANNNNNTGG 1 cut(s) 777
VpaK11BI GGWCC 1 cut(s) 456
XapI RAATTY 4 cut(s) 4, 518, 608, 856
XceI RCATGY 1 cut(s) 26
XmnI GAANNNNTTC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.