Rw2G007470

Transmembrane protein 184 homolog DDB_G0279555

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012223
Physical Location & Seq
Forward (+)
7286983 .. 7289320
2338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G007470.1

Sequence Viewer

Length: 1269 bp
ATGGCATCGTCTTCTTCACATACTTCAGTCCATGAAAACATATATGCAGATCTCTACAAACCAGCCGTGATAACTGCAGCTTCCTTTACAGTTGTGGCACTCATTCTCTCCCTTTTTCTCATCCTTCGGCATCTCAGATCATACAACAAGCCTTCAGAACAAATATGGGTGGTTGCTGTTGTTTTCATGGTCCCTGTCTATGCTACTGACTCGATGATATCCTTGTTGAATCCGAAGTTGTCTCTAGCATGTGACATTCTAAGAAGCTGTTATGAAGCGTTTGCCTTGTATTCTTTTGGAAGCTACTTGGTTGCTTGTCTGGGAGGTGAGAGAAGAGTAGTAGAATTACTTGAAAATCAATCTGGAAAGATCCTAAACAAAGCATTGGTAGAAGGGGAAGATGAGAATTGCAGGAAACCACACTGGTCTCTTCGCAACTTCTTCAGGCGACCAACTATTATTGGGAAAAATTTGCTCACTATAGTGAAATTCGGTCTTGTACAATATATGATTCTGAAGACAGTTTGTGCATTCCTAGCACTCGTGTTGGAAATCTTTGGCGTCTATGGTGATGGGGAATTCAAGTTTCACTACGGGTATCCATACATCACGATAGTACTGAATTTCAGCCAGATGTGGGCATTGTATTGCCTTGTGCAATTCTATAATGTGACTCATGAAAGACTTCAACCAATAAGGCCGCTTGCAAAGTTCATCAGCTTTAAGGCTATTGTGTTTGCGACTTGGTGGCAAGGTGTAGGTATTGCGCTGTTGTGTTATTTTGGTGTTCTTCCCAACCAGGAAAAACTACAAACAGGGTTGCAGGATTTCTTGATTTGTATAGAAATGGCAATTGCAGCTGTTGCACATGTGTTTGTCTTTTCGGCAGAACCTTACCATTACATTCCATCTTCTTCTGGGTATGAGAAGATCACCACCACTGAAACAACCAGTGAGGAAGCAGTGAAGTTGGAGGAAGGTGACAGTAGTACACAAGCTGTGGTTGAAACAAAAGAAACTCAGGTCGAGGCTCCGGGAACAAGTGTGACTGAGAGTGTTCAGGACATAGTGTTTGAAGGTGGTCATCATGTTGTCAAGGATGTTGTGTTGACCATAAATCAAGCAATAGGGCCTGTGGAGAAGGGTGTGACAAAGATACAAGAAACCTTCCACCAAAGATCAGATGAGCATGACGATAAGTCGAAGGTTTCAGTTGAGGAGAGTATTGAAGTGACTCTTTCCGATGAAGAAACACATTTGGACCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0016020 GO:0016021 GO:0031224 GO:0044425
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Solute_trans_a

Protein Analysis

422

Amino Acids

47.38

Weight (kDa)

5.42

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Solute_trans_a PF03619 23 - 299 4.5e-93 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23070
fragaria_vesca FvH4_1g08970 FvH4_1g08970 FvH4_1g08970 FvH4_1g08970 FvH4_1g08970 FvH4_1g08970
malus_domestica MD02G1095200.v1.1
prunus_persica Prupe.7G196000_v2.0.a1
pyrus_communis pycom02g07520
rosa_chinensis RchiOBHm_Chr2g0095421
rosa_laevigata RLG00000016564
rosa_multiflora Rmu_co8230917.1_g000001 Rmu_sc0010847.1_g000005
rosa_samantha Rh2AG098900 Rh2BG100100 Rh2CG102100 Rh2DG099700
rosa_wichuraiana Rw2G007470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 701
AclWI GGATC 1 cut(s) 364
AcsI RAATTY 4 cut(s) 469, 488, 578, 622
AcuI CTGAAG 4 cut(s) 9, 138, 427, 536
AcyI GRCGYC 1 cut(s) 561
AfaI GTAC 3 cut(s) 501, 618, 991
AfiI CCNNNNNNNGG 1 cut(s) 637
AflIII ACRYGT 1 cut(s) 868
AgsI TTSAA 7 cut(s) 229, 353, 583, 689, 1007, 1076, 1229
AhdI GACNNNNNGTC 1 cut(s) 1198
AjnI CCWGG 1 cut(s) 798
AleI CACNNNNGTG 1 cut(s) 482
AluBI AGCT 6 cut(s) 80, 267, 303, 720, 860, 998
AluI AGCT 6 cut(s) 80, 267, 303, 720, 860, 998
Alw26I GTCTC 2 cut(s) 246, 432
AlwI GGATC 1 cut(s) 364
AoxI GGCC 2 cut(s) 698, 1130
ApeKI GCWGC 2 cut(s) 77, 857
ApoI RAATTY 4 cut(s) 469, 488, 578, 622
ArsI GACNNNNNNTTYG 2 cut(s) 1055, 1087
Asp700I GAANNNNTTC 1 cut(s) 684
AspLEI GCGC 1 cut(s) 769
AspS9I GGNCC 3 cut(s) 190, 1130, 1261
AsuC2I CCSGG 1 cut(s) 1035
AsuHPI GGTGA 4 cut(s) 338, 581, 925, 992
AvaII GGWCC 2 cut(s) 190, 1261
BauI CACGAG 1 cut(s) 542
BbsI GAAGAC 2 cut(s) 3, 524
BbvI GCAGC 2 cut(s) 89, 869
BccI CCATC 2 cut(s) 566, 916
BceAI ACGGC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 800
BciVI GTATCC 1 cut(s) 609
BcnI CCSGG 1 cut(s) 1035
BcoDI GTCTC 2 cut(s) 246, 432
BfaI CTAG 2 cut(s) 245, 536
BfmI CTRYAG 2 cut(s) 75, 480
BfuI GTATCC 1 cut(s) 609
BglII AGATCT 1 cut(s) 49
BisI GCNGC 3 cut(s) 78, 701, 858
BlsI GCNGC 3 cut(s) 79, 702, 859
BmcAI AGTACT 1 cut(s) 618
Bme1390I CCNGG 2 cut(s) 800, 1035
Bme18I GGWCC 2 cut(s) 190, 1261
BmeRI GACNNNNNGTC 1 cut(s) 1198
BmgT120I GGNCC 3 cut(s) 190, 1130, 1261
BmiI GGNNCC 2 cut(s) 192, 1032
BmrFI CCNGG 2 cut(s) 800, 1035
BmsI GCATC 2 cut(s) 14, 139
BpiI GAAGAC 2 cut(s) 3, 524
BpuMI CCSGG 1 cut(s) 1035
BsaHI GRCGYC 1 cut(s) 561
BsaI GGTCTC 1 cut(s) 432
Bsc4I CCNNNNNNNGG 1 cut(s) 637
Bse1I ACTGG 2 cut(s) 428, 951
BseBI CCWGG 1 cut(s) 800
BseGI GGATG 2 cut(s) 120, 1105
BseLI CCNNNNNNNGG 1 cut(s) 637
BseMII CTCAG 3 cut(s) 148, 1034, 1041
BseNI ACTGG 2 cut(s) 428, 951
BseRI GAGGAG 1 cut(s) 1232
BseXI GCAGC 2 cut(s) 89, 869
BshFI GGCC 2 cut(s) 700, 1132
BsiSI CCGG 1 cut(s) 1034
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 1 cut(s) 637
BsmAI GTCTC 2 cut(s) 246, 432
BsmFI GGGAC 1 cut(s) 176
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 2 cut(s) 700, 1132
Bso31I GGTCTC 1 cut(s) 432
Bsp1407I TGTACA 1 cut(s) 499
Bsp143I GATC 5 cut(s) 49, 137, 369, 930, 1178
BspACI CCGC 1 cut(s) 701
BspANI GGCC 2 cut(s) 700, 1132
BspCNI CTCAG 3 cut(s) 147, 1033, 1042
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 2 cut(s) 192, 1032
BspMAI CTGCAG 1 cut(s) 79
BspPI GGATC 1 cut(s) 364
BspTNI GGTCTC 1 cut(s) 432
BsrGI TGTACA 1 cut(s) 499
BsrI ACTGG 2 cut(s) 428, 951
BssMI GATC 5 cut(s) 49, 137, 369, 930, 1178
BssNI GRCGYC 1 cut(s) 561
BssSI CACGAG 1 cut(s) 542
Bst2BI CACGAG 1 cut(s) 542
Bst2UI CCWGG 1 cut(s) 800
Bst4CI ACNGT 4 cut(s) 91, 523, 986, 1265
Bst6I CTCTTC 2 cut(s) 328, 435
BstACI GRCGYC 1 cut(s) 561
BstAPI GCANNNNNTGC 1 cut(s) 863
BstAUI TGTACA 1 cut(s) 499
BstC8I GCNNGC 1 cut(s) 705
BstDEI CTNAG 4 cut(s) 134, 260, 1020, 1050
BstF5I GGATG 2 cut(s) 120, 1105
BstHHI GCGC 1 cut(s) 769
BstKTI GATC 5 cut(s) 52, 140, 372, 933, 1181
BstMAI GTCTC 2 cut(s) 246, 432
BstMBI GATC 5 cut(s) 49, 137, 369, 930, 1178
BstMWI GCNNNNNNNGC 3 cut(s) 536, 857, 863
BstNI CCWGG 1 cut(s) 800
BstNSI RCATGY 2 cut(s) 252, 872
BstSCI CCNGG 2 cut(s) 798, 1033
BstSFI CTRYAG 2 cut(s) 75, 480
BstV1I GCAGC 2 cut(s) 89, 869
BstV2I GAAGAC 2 cut(s) 3, 524
BstX2I RGATCY 2 cut(s) 49, 369
BstYI RGATCY 2 cut(s) 49, 369
BsuI GTATCC 1 cut(s) 609
BsuRI GGCC 2 cut(s) 700, 1132
BtsCI GGATG 2 cut(s) 120, 1105
BtsI GCAGTG 1 cut(s) 969
BtsIMutI CAGTG 4 cut(s) 421, 939, 958, 969
Cac8I GCNNGC 1 cut(s) 705
CciI TCATGA 1 cut(s) 676
CfoI GCGC 1 cut(s) 769
Cfr13I GGNCC 3 cut(s) 190, 1130, 1261
CseI GACGC 1 cut(s) 550
Csp6I GTAC 3 cut(s) 500, 617, 990
CviAII CATG 7 cut(s) 32, 187, 249, 677, 869, 1088, 1190
CviQI GTAC 3 cut(s) 500, 617, 990
DdeI CTNAG 4 cut(s) 134, 260, 1020, 1050
DpnI GATC 5 cut(s) 51, 139, 371, 932, 1180
DpnII GATC 5 cut(s) 49, 137, 369, 930, 1178
DriI GACNNNNNGTC 1 cut(s) 1198
Eam1104I CTCTTC 2 cut(s) 328, 435
Eam1105I GACNNNNNGTC 1 cut(s) 1198
EarI CTCTTC 2 cut(s) 328, 435
Eco31I GGTCTC 1 cut(s) 432
Eco32I GATATC 1 cut(s) 219
Eco47I GGWCC 2 cut(s) 190, 1261
Eco57I CTGAAG 4 cut(s) 9, 138, 427, 536
EcoO109I RGGNCCY 1 cut(s) 1130
EcoRI GAATTC 1 cut(s) 578
EcoRII CCWGG 1 cut(s) 798
EcoRV GATATC 1 cut(s) 219
FaeI CATG 7 cut(s) 35, 190, 252, 680, 872, 1091, 1193
FalI AAGNNNNNCTT 2 cut(s) 1221, 1253
FaqI GGGAC 1 cut(s) 176
FatI CATG 7 cut(s) 31, 186, 248, 676, 868, 1087, 1189
Fnu4HI GCNGC 3 cut(s) 78, 701, 858
FokI GGATG 2 cut(s) 107, 1112
Fsp4HI GCNGC 3 cut(s) 78, 701, 858
FspBI CTAG 2 cut(s) 245, 536
GlaI GCGC 1 cut(s) 768
GluI GCNGC 3 cut(s) 78, 701, 858
HaeIII GGCC 2 cut(s) 700, 1132
HapII CCGG 1 cut(s) 1034
HgaI GACGC 1 cut(s) 550
HhaI GCGC 1 cut(s) 769
Hin1I GRCGYC 1 cut(s) 561
Hin1II CATG 7 cut(s) 35, 190, 252, 680, 872, 1091, 1193
Hin6I GCGC 1 cut(s) 767
HinP1I GCGC 1 cut(s) 767
HincII GTYRAC 1 cut(s) 1110
HindII GTYRAC 1 cut(s) 1110
HinfI GANTC 5 cut(s) 209, 229, 511, 673, 1234
HpaII CCGG 1 cut(s) 1034
HphI GGTGA 4 cut(s) 338, 581, 925, 992
Hpy166II GTNNAC 2 cut(s) 992, 1110
Hpy188I TCNGA 6 cut(s) 137, 157, 234, 516, 1183, 1243
Hpy188III TCNNGA 5 cut(s) 363, 610, 677, 832, 1061
Hpy8I GTNNAC 2 cut(s) 992, 1110
HpyAV CCTTC 8 cut(s) 134, 162, 386, 971, 1070, 1135, 1177, 1198
HpyCH4III ACNGT 4 cut(s) 91, 523, 986, 1265
HpyCH4V TGCA 9 cut(s) 47, 77, 411, 530, 658, 707, 823, 857, 866
HpyF10VI GCNNNNNNNGC 3 cut(s) 536, 857, 863
HpyF3I CTNAG 4 cut(s) 134, 260, 1020, 1050
Hsp92I GRCGYC 1 cut(s) 561
Hsp92II CATG 7 cut(s) 35, 190, 252, 680, 872, 1091, 1193
HspAI GCGC 1 cut(s) 767
Kzo9I GATC 5 cut(s) 49, 137, 369, 930, 1178
LmnI GCTCC 1 cut(s) 1036
Lsp1109I GCAGC 2 cut(s) 89, 869
LweI GCATC 2 cut(s) 14, 139
MaeI CTAG 2 cut(s) 245, 536
MaeIII GTNAC 6 cut(s) 251, 670, 980, 1045, 1147, 1231
MalI GATC 5 cut(s) 51, 139, 371, 932, 1180
MboI GATC 5 cut(s) 49, 137, 369, 930, 1178
MfeI CAATTG 1 cut(s) 852
MflI RGATCY 2 cut(s) 49, 369
MluCI AATT 8 cut(s) 344, 406, 469, 488, 578, 622, 659, 852
MlyI GAGTC 3 cut(s) 203, 667, 1228
MmeI TCCRAC 2 cut(s) 528, 951
MnlI CCTC 5 cut(s) 317, 949, 967, 1021, 1210
MroXI GAANNNNTTC 1 cut(s) 684
MseI TTAA 2 cut(s) 723, 1267
MslI CAYNNNNRTG 1 cut(s) 482
MspA1I CMGCKG 1 cut(s) 860
MspI CCGG 1 cut(s) 1034
MspR9I CCNGG 2 cut(s) 800, 1035
MunI CAATTG 1 cut(s) 852
Mva1269I GAATGC 1 cut(s) 530
MvaI CCWGG 1 cut(s) 800
MwoI GCNNNNNNNGC 3 cut(s) 536, 857, 863
NciI CCSGG 1 cut(s) 1035
NdeII GATC 5 cut(s) 49, 137, 369, 930, 1178
NlaIII CATG 7 cut(s) 35, 190, 252, 680, 872, 1091, 1193
NlaIV GGNNCC 2 cut(s) 192, 1032
NmuCI GTSAC 6 cut(s) 251, 670, 980, 1045, 1147, 1231
NspI RCATGY 2 cut(s) 252, 872
OliI CACNNNNGTG 1 cut(s) 482
PagI TCATGA 1 cut(s) 676
PciI ACATGT 1 cut(s) 868
PctI GAATGC 1 cut(s) 530
PdmI GAANNNNTTC 1 cut(s) 684
PfeI GAWTC 2 cut(s) 229, 511
PfoI TCCNGGA 1 cut(s) 1033
PkrI GCNGC 3 cut(s) 79, 702, 859
PleI GAGTC 3 cut(s) 203, 667, 1228
PpsI GAGTC 3 cut(s) 203, 667, 1228
PscI ACATGT 1 cut(s) 868
Psp6I CCWGG 1 cut(s) 798
PspGI CCWGG 1 cut(s) 798
PspN4I GGNNCC 2 cut(s) 192, 1032
PspPI GGNCC 3 cut(s) 190, 1130, 1261
PstI CTGCAG 1 cut(s) 79
PsuI RGATCY 2 cut(s) 49, 369
PvuII CAGCTG 1 cut(s) 860
RsaI GTAC 3 cut(s) 501, 618, 991
RsaNI GTAC 3 cut(s) 500, 617, 990
RseI CAYNNNNRTG 1 cut(s) 482
SaqAI TTAA 2 cut(s) 723, 1267
SatI GCNGC 3 cut(s) 78, 701, 858
Sau3AI GATC 5 cut(s) 49, 137, 369, 930, 1178
Sau96I GGNCC 3 cut(s) 190, 1130, 1261
ScaI AGTACT 1 cut(s) 618
SchI GAGTC 3 cut(s) 203, 667, 1228
ScrFI CCNGG 2 cut(s) 800, 1035
SfaNI GCATC 2 cut(s) 14, 139
SfcI CTRYAG 2 cut(s) 75, 480
SinI GGWCC 2 cut(s) 190, 1261
SmiMI CAYNNNNRTG 1 cut(s) 482
Sse9I AATT 8 cut(s) 344, 406, 469, 488, 578, 622, 659, 852
SsiI CCGC 1 cut(s) 701
SspMI CTAG 2 cut(s) 245, 536
StyD4I CCNGG 2 cut(s) 798, 1033
TaaI ACNGT 4 cut(s) 91, 523, 986, 1265
TaqI TCGA 3 cut(s) 212, 1026, 1202
TaqII GACCGA 1 cut(s) 482
TasI AATT 8 cut(s) 344, 406, 469, 488, 578, 622, 659, 852
TatI WGTACW 3 cut(s) 499, 616, 989
TauI GCSGC 1 cut(s) 703
TfiI GAWTC 2 cut(s) 229, 511
Tru1I TTAA 2 cut(s) 723, 1267
Tru9I TTAA 2 cut(s) 723, 1267
TscAI CASTG 4 cut(s) 428, 946, 958, 969
TseFI GTSAC 6 cut(s) 251, 670, 980, 1045, 1147, 1231
TseI GCWGC 2 cut(s) 77, 857
Tsp45I GTSAC 6 cut(s) 251, 670, 980, 1045, 1147, 1231
TspDTI ATGAA 6 cut(s) 48, 175, 288, 693, 703, 1260
TspRI CASTG 4 cut(s) 428, 946, 958, 969
VpaK11BI GGWCC 2 cut(s) 190, 1261
XapI RAATTY 4 cut(s) 469, 488, 578, 622
XceI RCATGY 2 cut(s) 252, 872
XmnI GAANNNNTTC 1 cut(s) 684
XspI CTAG 2 cut(s) 245, 536
ZrmI AGTACT 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.