Rh2DG099700

Transmembrane protein 184 homolog DDB_G0279555

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0012223
Physical Location & Seq
Forward (+)
8009987 .. 8013233
3247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG099700.1

Sequence Viewer

Length: 1200 bp
ATGGCATCGTCTTCTTCACATACTTCAGTCCATGAAAACATATATGCAGATCTCTACAAACCAGCCGTGATAACTGCAGCTTCCTTTACAGTTGTGGCACTCATTCTCTCCCTTTTTCTCATCCTTCGGCATCTCAGATCATACAACAAGCCTTCAGAACAAATATGGGTGGTTGCTGTTGTTTTCATGGTACCTGTCTATGCTACTGACTCGATATTAGAGCTTGCATTATTCATGAATGGCTATTACACAGGAGGTGAGAGAAGAGTAGTAGAATTACTTGAAAATCAATCTGGAAAGATCCTAAACAAAGCATTGGTAGAAGGGGAAGATGAGAATTGCAGGAAACCACACTGGTCTCTTCGCAACTTCTTCAGGCGACCAACTATTATTGGGAAAAATTTGCTCATTATAGTGAAATTCGGTCTTGTACAATATATGATTCTGAAGACAGTTTGTGCATTCCTAGCACTCGTGTTGGAAATCTTTGGCGTCTATGGTGATGGGGAATTCAAGTTTCAGTACGGGTATCCATACATCACGATAGTACTGAATTTCAGCCAGATGTGGGCATTGTATTGCCTTGTGCAATTCTATAATGTGACTCATGAAAGACTTCAACCAATAAGGCCGCTTGCAAAGTTCATCAGCTTTAAGGCTATTGTGTTTGCGACTTGGTGGCAAGGTGTAGGTATTGCGCTGTTGTGTTATTTTGGTGTTCTTCCAAACCAGGAAAAACTACAAACAGGGTTGCAGGATTTCTTGATTTGTATAGAAATGGCAATTGCAGCTGTTGCACATGTGTTTGTCTTTTCGGCAGAACCTTACCATTACATTCCATCTTCTTCTGGGTATGAGAAGATCACCACCACTGAAACAACCAGTGAGGAAGCAGTGAAGTTGGAGGAAGGTGACAGTAGCACACAAGCTGTGGTTGAAACAAAAGAAACTCAGGTCGAGGCTCCCGGAACAAGTGTGACTGAGAGTGTTCAGGACATAGTGTTTGAAGGTGGTCATCATGTTGTCAAGGATGTTGTGTTGACCATAAATCAAGCAATAGGGCCTGTGGAGAAGGGTGTGACAAAGATACAAGAAACCTTCCACCAAAGATCAGATGAGCATGACGATAAGTCGAAGGTTTCAGTTGAGGAGAGTATTGAAGTGACTCTTTCCGATGAAGAAACACATTTGGACCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0016020 GO:0016021 GO:0031224 GO:0044425
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Solute_trans_a

Protein Analysis

399

Amino Acids

44.94

Weight (kDa)

5.29

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Solute_trans_a PF03619 23 - 276 6.5e-72 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23070
fragaria_vesca FvH4_1g08970 FvH4_1g08970 FvH4_1g08970 FvH4_1g08970 FvH4_1g08970 FvH4_1g08970
malus_domestica MD02G1095200.v1.1
prunus_persica Prupe.7G196000_v2.0.a1
pyrus_communis pycom02g07520
rosa_chinensis RchiOBHm_Chr2g0095421
rosa_laevigata RLG00000016564
rosa_multiflora Rmu_co8230917.1_g000001 Rmu_sc0010847.1_g000005
rosa_samantha Rh2AG098900 Rh2BG100100 Rh2CG102100 Rh2DG099700
rosa_wichuraiana Rw2G007470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 190
AccB1I GGYRCC 1 cut(s) 190
AciI CCGC 1 cut(s) 632
AclWI GGATC 1 cut(s) 295
AcsI RAATTY 4 cut(s) 400, 419, 509, 553
AcuI CTGAAG 4 cut(s) 9, 138, 358, 467
AcyI GRCGYC 1 cut(s) 492
AfaI GTAC 4 cut(s) 192, 432, 524, 549
AfiI CCNNNNNNNGG 1 cut(s) 568
AflIII ACRYGT 1 cut(s) 799
AgsI TTSAA 6 cut(s) 284, 514, 620, 938, 1007, 1160
AhdI GACNNNNNGTC 1 cut(s) 1129
AjnI CCWGG 1 cut(s) 729
AluBI AGCT 5 cut(s) 80, 223, 651, 791, 929
AluI AGCT 5 cut(s) 80, 223, 651, 791, 929
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 1 cut(s) 295
AoxI GGCC 2 cut(s) 629, 1061
ApeKI GCWGC 2 cut(s) 77, 788
ApoI RAATTY 4 cut(s) 400, 419, 509, 553
ArsI GACNNNNNNTTYG 2 cut(s) 986, 1018
Asp700I GAANNNNTTC 1 cut(s) 615
Asp718I GGTACC 1 cut(s) 190
AspLEI GCGC 1 cut(s) 700
AspS9I GGNCC 2 cut(s) 1061, 1192
AsuC2I CCSGG 1 cut(s) 966
AsuHPI GGTGA 4 cut(s) 269, 512, 856, 923
AvaII GGWCC 1 cut(s) 1192
BanI GGYRCC 1 cut(s) 190
BauI CACGAG 1 cut(s) 473
BbsI GAAGAC 2 cut(s) 3, 455
BbvI GCAGC 2 cut(s) 89, 800
BccI CCATC 2 cut(s) 497, 847
BceAI ACGGC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 731
BciVI GTATCC 1 cut(s) 540
BcnI CCSGG 1 cut(s) 966
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 1 cut(s) 467
BfmI CTRYAG 1 cut(s) 75
BfuI GTATCC 1 cut(s) 540
BglII AGATCT 1 cut(s) 49
BisI GCNGC 3 cut(s) 78, 632, 789
BlsI GCNGC 3 cut(s) 79, 633, 790
BmcAI AGTACT 1 cut(s) 549
Bme1390I CCNGG 2 cut(s) 731, 966
Bme18I GGWCC 1 cut(s) 1192
BmeRI GACNNNNNGTC 1 cut(s) 1129
BmgT120I GGNCC 2 cut(s) 1061, 1192
BmiI GGNNCC 2 cut(s) 192, 963
BmrFI CCNGG 2 cut(s) 731, 966
BmsI GCATC 2 cut(s) 14, 139
BpiI GAAGAC 2 cut(s) 3, 455
BpuMI CCSGG 1 cut(s) 966
BsaHI GRCGYC 1 cut(s) 492
BsaI GGTCTC 1 cut(s) 363
Bsc4I CCNNNNNNNGG 1 cut(s) 568
Bse1I ACTGG 2 cut(s) 359, 882
BseBI CCWGG 1 cut(s) 731
BseGI GGATG 2 cut(s) 120, 1036
BseLI CCNNNNNNNGG 1 cut(s) 568
BseMII CTCAG 3 cut(s) 148, 965, 972
BseNI ACTGG 2 cut(s) 359, 882
BseRI GAGGAG 1 cut(s) 1163
BseXI GCAGC 2 cut(s) 89, 800
BshFI GGCC 2 cut(s) 631, 1063
BshNI GGYRCC 1 cut(s) 190
BsiSI CCGG 1 cut(s) 966
BslI CCNNNNNNNGG 1 cut(s) 568
BsmAI GTCTC 1 cut(s) 363
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 2 cut(s) 631, 1063
Bso31I GGTCTC 1 cut(s) 363
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 5 cut(s) 49, 137, 300, 861, 1109
BspACI CCGC 1 cut(s) 632
BspANI GGCC 2 cut(s) 631, 1063
BspCNI CTCAG 3 cut(s) 147, 964, 973
BspHI TCATGA 2 cut(s) 234, 607
BspLI GGNNCC 2 cut(s) 192, 963
BspMAI CTGCAG 1 cut(s) 79
BspPI GGATC 1 cut(s) 295
BspT107I GGYRCC 1 cut(s) 190
BspTNI GGTCTC 1 cut(s) 363
BsrGI TGTACA 1 cut(s) 430
BsrI ACTGG 2 cut(s) 359, 882
BssMI GATC 5 cut(s) 49, 137, 300, 861, 1109
BssNI GRCGYC 1 cut(s) 492
BssSI CACGAG 1 cut(s) 473
Bst2BI CACGAG 1 cut(s) 473
Bst2UI CCWGG 1 cut(s) 731
Bst4CI ACNGT 4 cut(s) 91, 454, 917, 1196
Bst6I CTCTTC 2 cut(s) 259, 366
BstACI GRCGYC 1 cut(s) 492
BstAPI GCANNNNNTGC 1 cut(s) 794
BstAUI TGTACA 1 cut(s) 430
BstC8I GCNNGC 2 cut(s) 225, 636
BstDEI CTNAG 3 cut(s) 134, 951, 981
BstF5I GGATG 2 cut(s) 120, 1036
BstHHI GCGC 1 cut(s) 700
BstKTI GATC 5 cut(s) 52, 140, 303, 864, 1112
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 5 cut(s) 49, 137, 300, 861, 1109
BstMWI GCNNNNNNNGC 3 cut(s) 467, 788, 794
BstNI CCWGG 1 cut(s) 731
BstNSI RCATGY 1 cut(s) 803
BstSCI CCNGG 2 cut(s) 729, 964
BstSFI CTRYAG 1 cut(s) 75
BstV1I GCAGC 2 cut(s) 89, 800
BstV2I GAAGAC 2 cut(s) 3, 455
BstX2I RGATCY 2 cut(s) 49, 300
BstYI RGATCY 2 cut(s) 49, 300
BsuI GTATCC 1 cut(s) 540
BsuRI GGCC 2 cut(s) 631, 1063
BtsCI GGATG 2 cut(s) 120, 1036
BtsI GCAGTG 1 cut(s) 900
BtsIMutI CAGTG 4 cut(s) 352, 870, 889, 900
Cac8I GCNNGC 2 cut(s) 225, 636
CciI TCATGA 2 cut(s) 234, 607
CfoI GCGC 1 cut(s) 700
Cfr13I GGNCC 2 cut(s) 1061, 1192
CseI GACGC 1 cut(s) 481
Csp6I GTAC 4 cut(s) 191, 431, 523, 548
CviAII CATG 7 cut(s) 32, 187, 235, 608, 800, 1019, 1121
CviQI GTAC 4 cut(s) 191, 431, 523, 548
DdeI CTNAG 3 cut(s) 134, 951, 981
DpnI GATC 5 cut(s) 51, 139, 302, 863, 1111
DpnII GATC 5 cut(s) 49, 137, 300, 861, 1109
DriI GACNNNNNGTC 1 cut(s) 1129
Eam1104I CTCTTC 2 cut(s) 259, 366
Eam1105I GACNNNNNGTC 1 cut(s) 1129
EarI CTCTTC 2 cut(s) 259, 366
Eco31I GGTCTC 1 cut(s) 363
Eco47I GGWCC 1 cut(s) 1192
Eco57I CTGAAG 4 cut(s) 9, 138, 358, 467
EcoO109I RGGNCCY 1 cut(s) 1061
EcoRI GAATTC 1 cut(s) 509
EcoRII CCWGG 1 cut(s) 729
FaeI CATG 7 cut(s) 35, 190, 238, 611, 803, 1022, 1124
FalI AAGNNNNNCTT 2 cut(s) 1152, 1184
FatI CATG 7 cut(s) 31, 186, 234, 607, 799, 1018, 1120
Fnu4HI GCNGC 3 cut(s) 78, 632, 789
FokI GGATG 2 cut(s) 107, 1043
Fsp4HI GCNGC 3 cut(s) 78, 632, 789
FspBI CTAG 1 cut(s) 467
GlaI GCGC 1 cut(s) 699
GluI GCNGC 3 cut(s) 78, 632, 789
HaeIII GGCC 2 cut(s) 631, 1063
HapII CCGG 1 cut(s) 966
HgaI GACGC 1 cut(s) 481
HhaI GCGC 1 cut(s) 700
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 7 cut(s) 35, 190, 238, 611, 803, 1022, 1124
Hin6I GCGC 1 cut(s) 698
HinP1I GCGC 1 cut(s) 698
HincII GTYRAC 1 cut(s) 1041
HindII GTYRAC 1 cut(s) 1041
HinfI GANTC 4 cut(s) 209, 442, 604, 1165
HpaII CCGG 1 cut(s) 966
HphI GGTGA 4 cut(s) 269, 512, 856, 923
Hpy166II GTNNAC 1 cut(s) 1041
Hpy188I TCNGA 5 cut(s) 137, 157, 447, 1114, 1174
Hpy188III TCNNGA 6 cut(s) 235, 294, 541, 608, 763, 992
Hpy8I GTNNAC 1 cut(s) 1041
HpyAV CCTTC 8 cut(s) 134, 162, 317, 902, 1001, 1066, 1108, 1129
HpyCH4III ACNGT 4 cut(s) 91, 454, 917, 1196
HpyF10VI GCNNNNNNNGC 3 cut(s) 467, 788, 794
HpyF3I CTNAG 3 cut(s) 134, 951, 981
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 7 cut(s) 35, 190, 238, 611, 803, 1022, 1124
HspAI GCGC 1 cut(s) 698
KpnI GGTACC 1 cut(s) 194
Kzo9I GATC 5 cut(s) 49, 137, 300, 861, 1109
LmnI GCTCC 1 cut(s) 967
Lsp1109I GCAGC 2 cut(s) 89, 800
LweI GCATC 2 cut(s) 14, 139
MaeI CTAG 1 cut(s) 467
MaeIII GTNAC 5 cut(s) 601, 911, 976, 1078, 1162
MalI GATC 5 cut(s) 51, 139, 302, 863, 1111
MboI GATC 5 cut(s) 49, 137, 300, 861, 1109
MfeI CAATTG 1 cut(s) 783
MflI RGATCY 2 cut(s) 49, 300
MluCI AATT 8 cut(s) 275, 337, 400, 419, 509, 553, 590, 783
MlyI GAGTC 3 cut(s) 203, 598, 1159
MmeI TCCRAC 2 cut(s) 459, 882
MnlI CCTC 5 cut(s) 248, 880, 898, 952, 1141
MroXI GAANNNNTTC 1 cut(s) 615
MseI TTAA 2 cut(s) 654, 1198
MslI CAYNNNNRTG 1 cut(s) 413
MspA1I CMGCKG 1 cut(s) 791
MspI CCGG 1 cut(s) 966
MspR9I CCNGG 2 cut(s) 731, 966
MunI CAATTG 1 cut(s) 783
Mva1269I GAATGC 1 cut(s) 461
MvaI CCWGG 1 cut(s) 731
MwoI GCNNNNNNNGC 3 cut(s) 467, 788, 794
NciI CCSGG 1 cut(s) 966
NdeII GATC 5 cut(s) 49, 137, 300, 861, 1109
NlaIII CATG 7 cut(s) 35, 190, 238, 611, 803, 1022, 1124
NlaIV GGNNCC 2 cut(s) 192, 963
NmuCI GTSAC 5 cut(s) 601, 911, 976, 1078, 1162
NspI RCATGY 1 cut(s) 803
PagI TCATGA 2 cut(s) 234, 607
PciI ACATGT 1 cut(s) 799
PctI GAATGC 1 cut(s) 461
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 1 cut(s) 442
PfoI TCCNGGA 1 cut(s) 964
PkrI GCNGC 3 cut(s) 79, 633, 790
PleI GAGTC 3 cut(s) 203, 598, 1159
PpsI GAGTC 3 cut(s) 203, 598, 1159
PscI ACATGT 1 cut(s) 799
Psp6I CCWGG 1 cut(s) 729
PspGI CCWGG 1 cut(s) 729
PspN4I GGNNCC 2 cut(s) 192, 963
PspPI GGNCC 2 cut(s) 1061, 1192
PstI CTGCAG 1 cut(s) 79
PsuI RGATCY 2 cut(s) 49, 300
PvuII CAGCTG 1 cut(s) 791
RsaI GTAC 4 cut(s) 192, 432, 524, 549
RsaNI GTAC 4 cut(s) 191, 431, 523, 548
RseI CAYNNNNRTG 1 cut(s) 413
SaqAI TTAA 2 cut(s) 654, 1198
SatI GCNGC 3 cut(s) 78, 632, 789
Sau3AI GATC 5 cut(s) 49, 137, 300, 861, 1109
Sau96I GGNCC 2 cut(s) 1061, 1192
ScaI AGTACT 1 cut(s) 549
SchI GAGTC 3 cut(s) 203, 598, 1159
ScrFI CCNGG 2 cut(s) 731, 966
SfaNI GCATC 2 cut(s) 14, 139
SfcI CTRYAG 1 cut(s) 75
SinI GGWCC 1 cut(s) 1192
SmiMI CAYNNNNRTG 1 cut(s) 413
Sse9I AATT 8 cut(s) 275, 337, 400, 419, 509, 553, 590, 783
SsiI CCGC 1 cut(s) 632
SspMI CTAG 1 cut(s) 467
StyD4I CCNGG 2 cut(s) 729, 964
TaaI ACNGT 4 cut(s) 91, 454, 917, 1196
TaqI TCGA 3 cut(s) 212, 957, 1133
TaqII GACCGA 1 cut(s) 413
TasI AATT 8 cut(s) 275, 337, 400, 419, 509, 553, 590, 783
TatI WGTACW 2 cut(s) 430, 547
TauI GCSGC 1 cut(s) 634
TfiI GAWTC 1 cut(s) 442
Tru1I TTAA 2 cut(s) 654, 1198
Tru9I TTAA 2 cut(s) 654, 1198
TscAI CASTG 4 cut(s) 359, 877, 889, 900
TseFI GTSAC 5 cut(s) 601, 911, 976, 1078, 1162
TseI GCWGC 2 cut(s) 77, 788
Tsp45I GTSAC 5 cut(s) 601, 911, 976, 1078, 1162
TspDTI ATGAA 7 cut(s) 48, 175, 223, 251, 624, 634, 1191
TspRI CASTG 4 cut(s) 359, 877, 889, 900
VpaK11BI GGWCC 1 cut(s) 1192
XapI RAATTY 4 cut(s) 400, 419, 509, 553
XceI RCATGY 1 cut(s) 803
XmnI GAANNNNTTC 1 cut(s) 615
XspI CTAG 1 cut(s) 467
ZrmI AGTACT 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.