MD02G1100000.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010995
Physical Location & Seq
Forward (+)
7936729 .. 7938938
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1100000.v1.1.491

Sequence Viewer

Length: 531 bp
ATGAATGAAAATGGTGGCACCAAGTTGACCGGAATCAGGCAGATTGTCAAGTTCAAAGAAATTCTGCAGAAGTGGCAAGCTGTCACGCTTGGCTCGAGGGCAAACAGCCCCCGAGCCAAAAGTAGTCCCCATTCTGAAAAAAGAGAAGGGGAGAGCACCACTCCCCGTTCTGAACGAGGCCATGGGGGCATTTCACCGGCAATTAGCAAGAGGCTAACAAATGTTATTTGTCATTCCGACGAGGATATATGCCACAGTCCCGAACCGCCACCTGATGTCCCCCAAGGGTATTTGGCGGTGTATGTTGGACCGGAGCTTCGGAGGTTCATCATTCCCACTAGCTACCTCAGTCACTCCCTTTTTAAAGTATTGCTGGAGAAGGCTGAGGAGGAATATGGGTTTGATCATGGCGGCGGGCTCACAATCCCATGTGAGACTGAGACCTTCAAGTACCTCTTGAAGTGCATGGAGAACCATCAGAAAGCTCACCCTGATGACATCCCAGCTGGAGACTGGAAACTCATTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

177

Amino Acids

19.65

Weight (kDa)

6.71

Isoelectric Point (pI)

49.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 74 - 155 2.1e-25 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010995)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G43120 AT5G20810 AT5G20810
fragaria_vesca FvH4_1g09280 FvH4_1g09280
malus_domestica MD02G1100000.v1.1 MD15G1222900.v1.1
prunus_persica Prupe.7G192900_v2.0.a1
pyrus_communis pycom02g07800 pycom15g19700
rosa_chinensis RchiOBHm_Chr2g0095841
rosa_laevigata RLG00000016599
rosa_multiflora Rmu_sc0000496.1_g000006
rosa_roxburghii Rroxscaffold_2G00146040
rosa_rugosa Rorug02G0053200
rosa_samantha Rh2AG102300 Rh2BG103700 Rh2CG106200 Rh2DG103800
rosa_wichuraiana Rw2G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 17
AciI CCGC 4 cut(s) 266, 296, 411, 414
AcsI RAATTY 1 cut(s) 60
AfaI GTAC 1 cut(s) 452
AfiI CCNNNNNNNGG 1 cut(s) 36
AgsI TTSAA 4 cut(s) 55, 448, 460, 527
AluBI AGCT 5 cut(s) 80, 316, 342, 485, 506
AluI AGCT 5 cut(s) 80, 316, 342, 485, 506
Alw21I GWGCWC 1 cut(s) 158
Alw26I GTCTC 3 cut(s) 428, 434, 504
Ama87I CYCGRG 2 cut(s) 94, 111
AoxI GGCC 1 cut(s) 178
ApoI RAATTY 1 cut(s) 60
ArsI GACNNNNNNTTYG 2 cut(s) 300, 332
AspS9I GGNCC 1 cut(s) 308
AsuHPI GGTGA 2 cut(s) 186, 479
AvaI CYCGRG 2 cut(s) 94, 111
AvaII GGWCC 1 cut(s) 308
BanI GGYRCC 1 cut(s) 17
BanII GRGCYC 1 cut(s) 420
Bbv12I GWGCWC 1 cut(s) 158
BbvCI CCTCAGC 1 cut(s) 384
BccI CCATC 1 cut(s) 483
BclI TGATCA 1 cut(s) 403
BcoDI GTCTC 3 cut(s) 428, 434, 504
BfaI CTAG 1 cut(s) 339
BfmI CTRYAG 1 cut(s) 65
BglI GCCNNNNNGGC 1 cut(s) 186
BisI GCNGC 1 cut(s) 412
BlsI GCNGC 1 cut(s) 413
Bme18I GGWCC 1 cut(s) 308
BmeT110I CYCGRG 2 cut(s) 94, 111
BmgT120I GGNCC 1 cut(s) 308
BmiI GGNNCC 1 cut(s) 19
BplI GAGNNNNNCTC 2 cut(s) 145, 177
BpmI CTGGAG 2 cut(s) 395, 528
Bpu10I CCTNAGC 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 434
BsaJI CCNNGG 2 cut(s) 181, 283
BsaWI WCCGGW 2 cut(s) 29, 310
Bsc4I CCNNNNNNNGG 1 cut(s) 36
Bse118I RCCGGY 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 518
BseDI CCNNGG 2 cut(s) 181, 283
BseGI GGATG 1 cut(s) 498
BseLI CCNNNNNNNGG 1 cut(s) 36
BseMII CTCAG 3 cut(s) 361, 375, 429
BseNI ACTGG 1 cut(s) 518
BseRI GAGGAG 1 cut(s) 401
BseYI CCCAGC 1 cut(s) 502
BshFI GGCC 1 cut(s) 180
BshNI GGYRCC 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 158
BsiHKCI CYCGRG 2 cut(s) 94, 111
BsiSI CCGG 3 cut(s) 30, 197, 311
BslFI GGGAC 3 cut(s) 111, 243, 263
BslI CCNNNNNNNGG 1 cut(s) 36
BsmAI GTCTC 3 cut(s) 428, 434, 504
BsmFI GGGAC 3 cut(s) 111, 243, 263
BsnI GGCC 1 cut(s) 180
Bso31I GGTCTC 1 cut(s) 434
BsoBI CYCGRG 2 cut(s) 94, 111
Bsp1286I GDGCHC 2 cut(s) 158, 420
Bsp143I GATC 1 cut(s) 403
Bsp19I CCATGG 1 cut(s) 181
BspACI CCGC 4 cut(s) 266, 296, 411, 414
BspANI GGCC 1 cut(s) 180
BspCNI CTCAG 3 cut(s) 360, 376, 430
BspLI GGNNCC 1 cut(s) 19
BspMAI CTGCAG 1 cut(s) 69
BspT107I GGYRCC 1 cut(s) 17
BspTNI GGTCTC 1 cut(s) 434
BsrFI RCCGGY 1 cut(s) 196
BsrI ACTGG 1 cut(s) 518
BssAI RCCGGY 1 cut(s) 196
BssECI CCNNGG 2 cut(s) 181, 283
BssMI GATC 1 cut(s) 403
BssT1I CCWWGG 2 cut(s) 181, 283
Bst4CI ACNGT 1 cut(s) 257
BstC8I GCNNGC 2 cut(s) 78, 416
BstDEI CTNAG 3 cut(s) 347, 384, 438
BstDSI CCRYGG 1 cut(s) 181
BstF5I GGATG 1 cut(s) 498
BstKTI GATC 1 cut(s) 406
BstMAI GTCTC 3 cut(s) 428, 434, 504
BstMBI GATC 1 cut(s) 403
BstMWI GCNNNNNNNGC 2 cut(s) 73, 186
BstSFI CTRYAG 1 cut(s) 65
BsuRI GGCC 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 181
BtsCI GGATG 1 cut(s) 498
Cac8I GCNNGC 2 cut(s) 78, 416
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 1 cut(s) 308
Csp6I GTAC 1 cut(s) 451
CviAII CATG 4 cut(s) 182, 407, 429, 466
CviQI GTAC 1 cut(s) 451
DdeI CTNAG 3 cut(s) 347, 384, 438
DpnI GATC 1 cut(s) 405
DpnII GATC 1 cut(s) 403
DraI TTTAAA 1 cut(s) 364
Eco130I CCWWGG 2 cut(s) 181, 283
Eco24I GRGCYC 1 cut(s) 420
Eco31I GGTCTC 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 308
Eco88I CYCGRG 2 cut(s) 94, 111
EcoT14I CCWWGG 2 cut(s) 181, 283
EcoT38I GRGCYC 1 cut(s) 420
ErhI CCWWGG 2 cut(s) 181, 283
FaeI CATG 4 cut(s) 185, 410, 432, 469
FaiI YATR 8 cut(s) 183, 248, 250, 303, 396, 408, 430, 467
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FaqI GGGAC 3 cut(s) 111, 243, 263
FatI CATG 4 cut(s) 181, 406, 428, 465
FauI CCCGC 1 cut(s) 407
FbaI TGATCA 1 cut(s) 403
Fnu4HI GCNGC 1 cut(s) 412
FokI GGATG 1 cut(s) 485
FriOI GRGCYC 1 cut(s) 420
Fsp4HI GCNGC 1 cut(s) 412
FspBI CTAG 1 cut(s) 339
GluI GCNGC 1 cut(s) 412
GsaI CCCAGC 1 cut(s) 506
GsuI CTGGAG 2 cut(s) 395, 528
HaeIII GGCC 1 cut(s) 180
HapII CCGG 3 cut(s) 30, 197, 311
Hin1II CATG 4 cut(s) 185, 410, 432, 469
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 1 cut(s) 33
HpaII CCGG 3 cut(s) 30, 197, 311
HphI GGTGA 2 cut(s) 186, 479
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 5 cut(s) 136, 172, 238, 321, 480
Hpy188III TCNNGA 2 cut(s) 260, 457
Hpy8I GTNNAC 1 cut(s) 27
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 3 cut(s) 140, 373, 454
HpyCH4III ACNGT 1 cut(s) 257
HpyCH4V TGCA 2 cut(s) 67, 465
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 186
HpyF3I CTNAG 3 cut(s) 347, 384, 438
Hsp92II CATG 4 cut(s) 185, 410, 432, 469
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 1 cut(s) 403
LmnI GCTCC 1 cut(s) 313
MaeI CTAG 1 cut(s) 339
MaeIII GTNAC 2 cut(s) 82, 350
MalI GATC 1 cut(s) 405
MboI GATC 1 cut(s) 403
MhlI GDGCHC 2 cut(s) 158, 420
MluCI AATT 2 cut(s) 60, 201
MmeI TCCRAC 2 cut(s) 261, 286
MnlI CCTC 9 cut(s) 90, 170, 204, 235, 315, 356, 379, 382, 464
MseI TTAA 1 cut(s) 363
MslI CAYNNNNRTG 1 cut(s) 492
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 3 cut(s) 30, 197, 311
MwoI GCNNNNNNNGC 2 cut(s) 73, 186
NcoI CCATGG 1 cut(s) 181
NdeII GATC 1 cut(s) 403
NlaIII CATG 4 cut(s) 185, 410, 432, 469
NlaIV GGNNCC 1 cut(s) 19
NmuCI GTSAC 2 cut(s) 82, 350
PaeR7I CTCGAG 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PfeI GAWTC 1 cut(s) 33
PkrI GCNGC 1 cut(s) 413
PspFI CCCAGC 1 cut(s) 502
PspN4I GGNNCC 1 cut(s) 19
PspPI GGNCC 1 cut(s) 308
PspXI VCTCGAGB 1 cut(s) 94
PstI CTGCAG 1 cut(s) 69
PvuII CAGCTG 1 cut(s) 506
RsaI GTAC 1 cut(s) 452
RsaNI GTAC 1 cut(s) 451
RseI CAYNNNNRTG 1 cut(s) 492
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 1 cut(s) 412
Sau3AI GATC 1 cut(s) 403
Sau96I GGNCC 1 cut(s) 308
SduI GDGCHC 2 cut(s) 158, 420
SfcI CTRYAG 1 cut(s) 65
Sfr274I CTCGAG 1 cut(s) 94
SinI GGWCC 1 cut(s) 308
SlaI CTCGAG 1 cut(s) 94
SmiMI CAYNNNNRTG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 2 cut(s) 60, 201
SsiI CCGC 4 cut(s) 266, 296, 411, 414
SspMI CTAG 1 cut(s) 339
StyI CCWWGG 2 cut(s) 181, 283
TaaI ACNGT 1 cut(s) 257
TaqI TCGA 1 cut(s) 95
TasI AATT 2 cut(s) 60, 201
TauI GCSGC 1 cut(s) 414
TfiI GAWTC 1 cut(s) 33
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TseFI GTSAC 2 cut(s) 82, 350
Tsp45I GTSAC 2 cut(s) 82, 350
TspDTI ATGAA 3 cut(s) 17, 21, 316
VpaK11BI GGWCC 1 cut(s) 308
XapI RAATTY 1 cut(s) 60
XcmI CCANNNNNNNNNTGG 1 cut(s) 510
XhoI CTCGAG 1 cut(s) 94
XspI CTAG 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.