MD15G1222900.v1.1

auxin-responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010995
Physical Location & Seq
Forward (+)
18103292 .. 18105613
2322 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1222900.v1.1.491

Sequence Viewer

Length: 555 bp
ATGAATGAAAATGGTGGCACCAAGTTGACTGGAATCAGGCAGATCGTCAAGTTCAAAGAAATCCTGCAGAAGTGGCAAGCCGTCACGCTTGGCTCAAGGGCAAACAGCCCCCGAGCCAACAGTAGTCCCCATTCTGAAAGAAGAGGAGGGGATCCCCATTCTGAAAAAAGAGGAGGGGAGAGCACCACTCCCTCTGAACGAGGCCATGGGGGTATTTCACCGGCAATTAGCCAGAGGCTAACAAATGTTATTTGTGACTCGGATGAGGATATATGCTACAGTCCCGAACCGCCACATGATATCCCCAAAGGATATTTGGCGGTTTATGTTGGACCGGAGCTTCGGAGGTTCATCATTCCCACTAGCTACCTCAGTCACTCCCTTTTTAAAGTATTGCTGGAGAAGGCTGAGGAGGAATTTGGGTTTGATCATAGCGGCGGGCTCACAATCCCATGTGAGACTGAGACCTTCAAGTACCTCTTGAAGTGCATGGAGAACCATCAGAAAGCTCACCCCGATGACAGCCCAGCCGGAGACTGGAAAGTCATTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

185

Amino Acids

20.43

Weight (kDa)

6.24

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 93 - 163 7.2e-26 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010995)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G43120 AT5G20810 AT5G20810
fragaria_vesca FvH4_1g09280 FvH4_1g09280
malus_domestica MD02G1100000.v1.1 MD15G1222900.v1.1
prunus_persica Prupe.7G192900_v2.0.a1
pyrus_communis pycom02g07800 pycom15g19700
rosa_chinensis RchiOBHm_Chr2g0095841
rosa_laevigata RLG00000016599
rosa_multiflora Rmu_sc0000496.1_g000006
rosa_roxburghii Rroxscaffold_2G00146040
rosa_rugosa Rorug02G0053200
rosa_samantha Rh2AG102300 Rh2BG103700 Rh2CG106200 Rh2DG103800
rosa_wichuraiana Rw2G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 542
AccB1I GGYRCC 1 cut(s) 17
AciI CCGC 4 cut(s) 290, 320, 435, 438
AclWI GGATC 2 cut(s) 146, 159
AcsI RAATTY 1 cut(s) 416
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 1 cut(s) 537
AgsI TTSAA 4 cut(s) 55, 472, 484, 551
AluBI AGCT 3 cut(s) 340, 366, 509
AluI AGCT 3 cut(s) 340, 366, 509
Alw21I GWGCWC 1 cut(s) 185
Alw26I GTCTC 3 cut(s) 452, 458, 528
AlwI GGATC 2 cut(s) 146, 159
Ama87I CYCGRG 1 cut(s) 111
AoxI GGCC 1 cut(s) 202
ApoI RAATTY 1 cut(s) 416
ArsI GACNNNNNNTTYG 2 cut(s) 324, 356
AspS9I GGNCC 1 cut(s) 332
AsuHPI GGTGA 2 cut(s) 210, 503
AvaI CYCGRG 1 cut(s) 111
AvaII GGWCC 1 cut(s) 332
BamHI GGATCC 1 cut(s) 151
BanI GGYRCC 1 cut(s) 17
BanII GRGCYC 1 cut(s) 444
Bbv12I GWGCWC 1 cut(s) 185
BbvCI CCTCAGC 1 cut(s) 408
BccI CCATC 1 cut(s) 507
BceAI ACGGC 1 cut(s) 65
BclI TGATCA 1 cut(s) 427
BcoDI GTCTC 3 cut(s) 452, 458, 528
BfaI CTAG 1 cut(s) 363
BfmI CTRYAG 2 cut(s) 65, 277
BisI GCNGC 1 cut(s) 436
BlsI GCNGC 1 cut(s) 437
Bme18I GGWCC 1 cut(s) 332
BmeT110I CYCGRG 1 cut(s) 111
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 2 cut(s) 19, 153
BplI GAGNNNNNCTC 2 cut(s) 172, 204
BpmI CTGGAG 1 cut(s) 419
Bpu10I CCTNAGC 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 79
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 1 cut(s) 205
BsaWI WCCGGW 1 cut(s) 334
Bsc4I CCNNNNNNNGG 1 cut(s) 537
Bse118I RCCGGY 1 cut(s) 220
Bse1I ACTGG 2 cut(s) 34, 542
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 1 cut(s) 268
BseLI CCNNNNNNNGG 1 cut(s) 537
BseMII CTCAG 3 cut(s) 385, 399, 453
BseNI ACTGG 2 cut(s) 34, 542
BseRI GAGGAG 3 cut(s) 159, 186, 425
BseYI CCCAGC 1 cut(s) 526
BshFI GGCC 1 cut(s) 204
BshNI GGYRCC 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 185
BsiHKCI CYCGRG 1 cut(s) 111
BsiSI CCGG 3 cut(s) 221, 335, 531
BslFI GGGAC 2 cut(s) 111, 267
BslI CCNNNNNNNGG 1 cut(s) 537
BsmAI GTCTC 3 cut(s) 452, 458, 528
BsmFI GGGAC 2 cut(s) 111, 267
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 458
BsoBI CYCGRG 1 cut(s) 111
Bsp1286I GDGCHC 2 cut(s) 185, 444
Bsp143I GATC 3 cut(s) 42, 151, 427
Bsp19I CCATGG 1 cut(s) 205
BspACI CCGC 4 cut(s) 290, 320, 435, 438
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 3 cut(s) 384, 400, 454
BspLI GGNNCC 2 cut(s) 19, 153
BspMAI CTGCAG 1 cut(s) 69
BspPI GGATC 2 cut(s) 146, 159
BspT107I GGYRCC 1 cut(s) 17
BspTNI GGTCTC 1 cut(s) 458
BsrFI RCCGGY 1 cut(s) 220
BsrI ACTGG 2 cut(s) 34, 542
BssAI RCCGGY 1 cut(s) 220
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 3 cut(s) 42, 151, 427
BssT1I CCWWGG 1 cut(s) 205
Bst4CI ACNGT 2 cut(s) 122, 281
Bst6I CTCTTC 1 cut(s) 136
BstC8I GCNNGC 2 cut(s) 78, 440
BstDEI CTNAG 3 cut(s) 371, 408, 462
BstDSI CCRYGG 1 cut(s) 205
BstF5I GGATG 1 cut(s) 268
BstKTI GATC 3 cut(s) 45, 154, 430
BstMAI GTCTC 3 cut(s) 452, 458, 528
BstMBI GATC 3 cut(s) 42, 151, 427
BstMWI GCNNNNNNNGC 1 cut(s) 73
BstSFI CTRYAG 2 cut(s) 65, 277
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BsuRI GGCC 1 cut(s) 204
BtgI CCRYGG 1 cut(s) 205
BtsCI GGATG 1 cut(s) 268
Cac8I GCNNGC 2 cut(s) 78, 440
Cfr10I RCCGGY 1 cut(s) 220
Cfr13I GGNCC 1 cut(s) 332
Csp6I GTAC 1 cut(s) 475
CviAII CATG 4 cut(s) 206, 296, 453, 490
CviQI GTAC 1 cut(s) 475
DdeI CTNAG 3 cut(s) 371, 408, 462
DpnI GATC 3 cut(s) 44, 153, 429
DpnII GATC 3 cut(s) 42, 151, 427
DraI TTTAAA 1 cut(s) 388
DrdI GACNNNNNNGTC 1 cut(s) 542
DseDI GACNNNNNNGTC 1 cut(s) 542
Eam1104I CTCTTC 1 cut(s) 136
EarI CTCTTC 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 205
Eco24I GRGCYC 1 cut(s) 444
Eco31I GGTCTC 1 cut(s) 458
Eco32I GATATC 1 cut(s) 301
Eco47I GGWCC 1 cut(s) 332
Eco88I CYCGRG 1 cut(s) 111
EcoRV GATATC 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 444
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 4 cut(s) 209, 299, 456, 493
FaiI YATR 8 cut(s) 207, 272, 274, 297, 327, 432, 454, 491
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 2 cut(s) 111, 267
FatI CATG 4 cut(s) 205, 295, 452, 489
FauI CCCGC 1 cut(s) 431
FbaI TGATCA 1 cut(s) 427
Fnu4HI GCNGC 1 cut(s) 436
FokI GGATG 1 cut(s) 275
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 1 cut(s) 436
FspBI CTAG 1 cut(s) 363
GluI GCNGC 1 cut(s) 436
GsaI CCCAGC 1 cut(s) 530
GsuI CTGGAG 1 cut(s) 419
HaeIII GGCC 1 cut(s) 204
HapII CCGG 3 cut(s) 221, 335, 531
Hin1II CATG 4 cut(s) 209, 299, 456, 493
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 2 cut(s) 33, 257
HpaII CCGG 3 cut(s) 221, 335, 531
HphI GGTGA 2 cut(s) 210, 503
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 6 cut(s) 136, 163, 196, 262, 345, 504
Hpy188III TCNNGA 2 cut(s) 284, 481
Hpy8I GTNNAC 1 cut(s) 27
HpyAV CCTTC 2 cut(s) 397, 478
HpyCH4III ACNGT 2 cut(s) 122, 281
HpyCH4V TGCA 2 cut(s) 67, 489
HpyF10VI GCNNNNNNNGC 1 cut(s) 73
HpyF3I CTNAG 3 cut(s) 371, 408, 462
Hsp92II CATG 4 cut(s) 209, 299, 456, 493
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 3 cut(s) 42, 151, 427
LmnI GCTCC 1 cut(s) 337
MaeI CTAG 1 cut(s) 363
MaeIII GTNAC 3 cut(s) 82, 254, 374
MalI GATC 3 cut(s) 44, 153, 429
MboI GATC 3 cut(s) 42, 151, 427
MboII GAAGA 1 cut(s) 153
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 2 cut(s) 185, 444
MluCI AATT 2 cut(s) 225, 416
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 1 cut(s) 310
MseI TTAA 1 cut(s) 387
MslI CAYNNNNRTG 1 cut(s) 516
MspI CCGG 3 cut(s) 221, 335, 531
MwoI GCNNNNNNNGC 1 cut(s) 73
NcoI CCATGG 1 cut(s) 205
NdeII GATC 3 cut(s) 42, 151, 427
NlaIII CATG 4 cut(s) 209, 299, 456, 493
NlaIV GGNNCC 2 cut(s) 19, 153
NmuCI GTSAC 3 cut(s) 82, 254, 374
PfeI GAWTC 1 cut(s) 33
PkrI GCNGC 1 cut(s) 437
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
PspFI CCCAGC 1 cut(s) 526
PspN4I GGNNCC 2 cut(s) 19, 153
PspPI GGNCC 1 cut(s) 332
PstI CTGCAG 1 cut(s) 69
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
RseI CAYNNNNRTG 1 cut(s) 516
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 1 cut(s) 436
Sau3AI GATC 3 cut(s) 42, 151, 427
Sau96I GGNCC 1 cut(s) 332
SchI GAGTC 1 cut(s) 251
SduI GDGCHC 2 cut(s) 185, 444
SetI ASST 7 cut(s) 342, 350, 368, 372, 470, 480, 511
SfcI CTRYAG 2 cut(s) 65, 277
SinI GGWCC 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 516
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 2 cut(s) 225, 416
SsiI CCGC 4 cut(s) 290, 320, 435, 438
SspMI CTAG 1 cut(s) 363
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 2 cut(s) 122, 281
TasI AATT 2 cut(s) 225, 416
TauI GCSGC 1 cut(s) 438
TfiI GAWTC 1 cut(s) 33
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TseFI GTSAC 3 cut(s) 82, 254, 374
Tsp45I GTSAC 3 cut(s) 82, 254, 374
TspDTI ATGAA 3 cut(s) 17, 21, 340
VpaK11BI GGWCC 1 cut(s) 332
XapI RAATTY 1 cut(s) 416
XcmI CCANNNNNNNNNTGG 2 cut(s) 313, 534
XspI CTAG 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.