MD02G1107600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008426
Physical Location & Seq
Forward (+)
8684690 .. 8686668
1979 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1107600.v1.1.491

Sequence Viewer

Length: 573 bp
ATGGCTGCAACCGTGTTGTTTGCATGCCATTCCAATGTAATTGTAAGCCACTGCTGTAGAAATGATTATCCGTCGTTACCCAAATTGTTCTTGGGTGTTGGACACAGAATCGTGGATCAAACCATTGCCAACAGAAATCAAAACCCTCAAAGCCTTGAAAAAAAGAAGTCATCTTATAGGTTTTCCGTTTCTGCTGTCACCAAAGGTTCTGCAAAATCAAGCAAGTCCGATGAGAAAATCCCCCCATGGGCTAAACCGGATTCAGACGAACCCCCTCCTTGGGCCCAGAACGAAGGCAAGAAGGATGATTCACAAGAGGGATTTGAGATTCCATTTTTTGTTTATCTGCTCGCCTCAGCAATCACTGCAATCGCTGCAATAGGTTCTGTTTTCGAGTATGTGAACGAGAAACCAGTCTTCGGAATCTTGAGTTCAGACAGCATATTTTATGCTCCAGTGCTTGGATTTTTTGCTTTTACTGGCATCCCCACTTCTGCATTCCTGTGGTTCAAATCTGTTCAAACTGCTAACAAGGAAGCTGAGGAGCAAGACAAGAGGGATGGCTATCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

21.02

Weight (kDa)

6.83

Isoelectric Point (pI)

37.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 461
AclWI GGATC 1 cut(s) 123
AfiI CCNNNNNNNGG 5 cut(s) 247, 279, 280, 419, 461
AgsI TTSAA 3 cut(s) 158, 511, 521
AluBI AGCT 1 cut(s) 539
AluI AGCT 1 cut(s) 539
AlwI GGATC 1 cut(s) 123
AoxI GGCC 1 cut(s) 282
ApaI GGGCCC 1 cut(s) 286
ApeKI GCWGC 2 cut(s) 5, 374
AspS9I GGNCC 2 cut(s) 282, 283
AsuHPI GGTGA 1 cut(s) 190
BaeGI GKGCMC 1 cut(s) 286
BanII GRGCYC 1 cut(s) 286
BbsI GAAGAC 1 cut(s) 409
BbvCI CCTCAGC 2 cut(s) 355, 540
BbvI GCAGC 1 cut(s) 361
BccI CCATC 1 cut(s) 554
BfmI CTRYAG 1 cut(s) 55
BisI GCNGC 2 cut(s) 6, 375
BlsI GCNGC 2 cut(s) 7, 376
BmgT120I GGNCC 2 cut(s) 282, 283
BmiI GGNNCC 1 cut(s) 284
BmsI GCATC 1 cut(s) 492
BpiI GAAGAC 1 cut(s) 409
BpmI CTGGAG 1 cut(s) 438
Bpu10I CCTNAGC 2 cut(s) 355, 540
BpuEI CTTGAG 1 cut(s) 448
Bsa29I ATCGAT 1 cut(s) 568
BsaBI GATNNNNATC 1 cut(s) 564
BsaJI CCNNGG 2 cut(s) 245, 278
BsaWI WCCGGW 1 cut(s) 256
Bsc4I CCNNNNNNNGG 5 cut(s) 247, 279, 280, 419, 461
Bse1I ACTGG 3 cut(s) 413, 455, 484
Bse3DI GCAATG 1 cut(s) 123
Bse8I GATNNNNATC 1 cut(s) 564
BseCI ATCGAT 1 cut(s) 568
BseDI CCNNGG 2 cut(s) 245, 278
BseGI GGATG 3 cut(s) 310, 483, 565
BseJI GATNNNNATC 1 cut(s) 564
BseLI CCNNNNNNNGG 5 cut(s) 247, 279, 280, 419, 461
BseMI GCAATG 1 cut(s) 123
BseMII CTCAG 2 cut(s) 369, 531
BseNI ACTGG 3 cut(s) 413, 455, 484
BseRI GAGGAG 1 cut(s) 557
BseSI GKGCMC 1 cut(s) 286
BseXI GCAGC 1 cut(s) 361
BshFI GGCC 1 cut(s) 284
BshVI ATCGAT 1 cut(s) 568
BsiSI CCGG 1 cut(s) 257
BslI CCNNNNNNNGG 5 cut(s) 247, 279, 280, 419, 461
BsmI GAATGC 1 cut(s) 497
BsnI GGCC 1 cut(s) 284
Bsp120I GGGCCC 1 cut(s) 282
Bsp1286I GDGCHC 1 cut(s) 286
Bsp143I GATC 1 cut(s) 115
Bsp19I CCATGG 1 cut(s) 245
BspANI GGCC 1 cut(s) 284
BspCNI CTCAG 2 cut(s) 368, 532
BspDI ATCGAT 1 cut(s) 568
BspLI GGNNCC 1 cut(s) 284
BspPI GGATC 1 cut(s) 123
BsrDI GCAATG 1 cut(s) 123
BsrI ACTGG 3 cut(s) 413, 455, 484
BssECI CCNNGG 2 cut(s) 245, 278
BssMI GATC 1 cut(s) 115
BssT1I CCWWGG 2 cut(s) 245, 278
Bst4CI ACNGT 1 cut(s) 13
BstAPI GCANNNNNTGC 2 cut(s) 365, 374
BstC8I GCNNGC 2 cut(s) 25, 351
BstDEI CTNAG 2 cut(s) 355, 540
BstDSI CCRYGG 1 cut(s) 245
BstF5I GGATG 3 cut(s) 310, 483, 565
BstKTI GATC 1 cut(s) 118
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 2 cut(s) 365, 374
BstNSI RCATGY 1 cut(s) 27
BstSFI CTRYAG 1 cut(s) 55
BstSLI GKGCMC 1 cut(s) 286
BstV1I GCAGC 1 cut(s) 361
BstV2I GAAGAC 1 cut(s) 409
Bsu15I ATCGAT 1 cut(s) 568
BsuRI GGCC 1 cut(s) 284
BsuTUI ATCGAT 1 cut(s) 568
BtgI CCRYGG 1 cut(s) 245
BtsCI GGATG 3 cut(s) 310, 483, 565
BtsI GCAGTG 2 cut(s) 49, 363
BtsIMutI CAGTG 3 cut(s) 49, 363, 462
Cac8I GCNNGC 2 cut(s) 25, 351
Cfr13I GGNCC 2 cut(s) 282, 283
ClaI ATCGAT 1 cut(s) 568
CviAII CATG 2 cut(s) 24, 246
CviJI RGCY 7 cut(s) 5, 48, 153, 251, 284, 539, 564
CviKI_1 RGCY 7 cut(s) 5, 48, 153, 251, 284, 539, 564
DdeI CTNAG 2 cut(s) 355, 540
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
Eco130I CCWWGG 2 cut(s) 245, 278
Eco24I GRGCYC 1 cut(s) 286
EcoT14I CCWWGG 2 cut(s) 245, 278
EcoT38I GRGCYC 1 cut(s) 286
ErhI CCWWGG 2 cut(s) 245, 278
FaeI CATG 2 cut(s) 27, 249
FaiI YATR 6 cut(s) 25, 177, 247, 399, 443, 450
FatI CATG 2 cut(s) 23, 245
Fnu4HI GCNGC 2 cut(s) 6, 375
FokI GGATG 2 cut(s) 317, 470
FriOI GRGCYC 1 cut(s) 286
Fsp4HI GCNGC 2 cut(s) 6, 375
GluI GCNGC 2 cut(s) 6, 375
GsuI CTGGAG 1 cut(s) 438
HaeIII GGCC 1 cut(s) 284
HapII CCGG 1 cut(s) 257
Hin1II CATG 2 cut(s) 27, 249
HinfI GANTC 5 cut(s) 108, 260, 308, 328, 423
HpaII CCGG 1 cut(s) 257
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 1 cut(s) 403
Hpy188I TCNGA 4 cut(s) 229, 265, 422, 436
Hpy188III TCNNGA 1 cut(s) 427
Hpy8I GTNNAC 1 cut(s) 403
Hpy99I CGWCG 1 cut(s) 76
HpyAV CCTTC 2 cut(s) 287, 295
HpyCH4III ACNGT 1 cut(s) 13
HpyCH4V TGCA 6 cut(s) 8, 23, 212, 368, 377, 497
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 374
HpyF3I CTNAG 2 cut(s) 355, 540
Hsp92II CATG 2 cut(s) 27, 249
Kzo9I GATC 1 cut(s) 115
LmnI GCTCC 2 cut(s) 457, 544
LpnPI CCDG 6 cut(s) 270, 299, 426, 465, 468, 515
Lsp1109I GCAGC 1 cut(s) 361
LweI GCATC 1 cut(s) 492
MaeIII GTNAC 2 cut(s) 75, 196
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 1 cut(s) 409
MhlI GDGCHC 1 cut(s) 286
MluCI AATT 2 cut(s) 39, 83
MmeI TCCRAC 1 cut(s) 79
MnlI CCTC 6 cut(s) 156, 285, 310, 364, 535, 549
MslI CAYNNNNRTG 2 cut(s) 33, 502
MspI CCGG 1 cut(s) 257
Mva1269I GAATGC 1 cut(s) 497
MwoI GCNNNNNNNGC 2 cut(s) 365, 374
NcoI CCATGG 1 cut(s) 245
NdeII GATC 1 cut(s) 115
NlaIII CATG 2 cut(s) 27, 249
NlaIV GGNNCC 1 cut(s) 284
NmuCI GTSAC 1 cut(s) 196
NspI RCATGY 1 cut(s) 27
PaeI GCATGC 1 cut(s) 27
PctI GAATGC 1 cut(s) 497
PfeI GAWTC 5 cut(s) 108, 260, 308, 328, 423
PflMI CCANNNNNTGG 1 cut(s) 461
PkrI GCNGC 2 cut(s) 7, 376
PspN4I GGNNCC 1 cut(s) 284
PspOMI GGGCCC 1 cut(s) 282
PspPI GGNCC 2 cut(s) 282, 283
RseI CAYNNNNRTG 2 cut(s) 33, 502
SatI GCNGC 2 cut(s) 6, 375
Sau3AI GATC 1 cut(s) 115
Sau96I GGNCC 2 cut(s) 282, 283
SduI GDGCHC 1 cut(s) 286
SetI ASST 4 cut(s) 182, 208, 385, 541
SfaNI GCATC 1 cut(s) 492
SfcI CTRYAG 1 cut(s) 55
SmiMI CAYNNNNRTG 2 cut(s) 33, 502
SmlI CTYRAG 1 cut(s) 427
SmoI CTYRAG 1 cut(s) 427
SphI GCATGC 1 cut(s) 27
Sse9I AATT 2 cut(s) 39, 83
StyI CCWWGG 2 cut(s) 245, 278
TaaI ACNGT 1 cut(s) 13
TaqI TCGA 2 cut(s) 393, 568
TasI AATT 2 cut(s) 39, 83
TfiI GAWTC 5 cut(s) 108, 260, 308, 328, 423
TscAI CASTG 3 cut(s) 56, 370, 462
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 2 cut(s) 5, 374
Tsp45I GTSAC 1 cut(s) 196
TspGWI ACGGA 2 cut(s) 60, 175
TspRI CASTG 3 cut(s) 56, 370, 462
Van91I CCANNNNNTGG 1 cut(s) 461
XceI RCATGY 1 cut(s) 27
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.