Rorug02G0059300

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008426
Physical Location & Seq
Forward (+)
4567904 .. 4575369
7466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0059300.1

Sequence Viewer

Length: 3234 bp
ATGGGTTGCGTGTATTCGAGGGTCTGTATTGGAGAGGTTAGTAGTTCAAGAGATGCGAGAAGGAAGGAGGCCCAGAATGCGAGGAACACTGAGATCCCAGTTTTCTCACCCGACTCTTCAAATGAGGAAGATGGTGATCAGTTCAACCAGGCAAACCATAGCAGAGATGCTGAAATGGGGATCACTAGGCTGTCGAGGGTTTCGGCACAGTTTCTACCTCCAAATGGGTCCAGAACTGTGAAGGTTCCTTCCGGCAAGTTTGAGTTGAGGTACTCGTATTTGTCCCAGAGAGGGTATTACCCTGATGCTCTCGATAAGGCTAACCAGGACAGCTTTTGCATCCACACTCCGTTTGGGACGAACCCGGATGACCATTTTTTCGGGGTGTTTGATGGCCATGGGGAGTTTGGAACCGAGTGTTCGCAGTTTGTCAAGAGGAAGTTGTGTGAGAATTTGCTTAGGCATAGTAAGTTTCAAGCGGATGCTGTCGAGGCGTGTCGCGAGGCGTTTCTTGCCACCAATTCGCAGTTGCATGATGACGACATTGTGGATGATAGCATGAGTGGAACGACAGCCATTACGGTTTTGGTTAGAGGTAGGAAGATATACATTGCCAATTCGGGGGATTCTAGGGCTGTTATAGCCGAGAGGAAAGGGGAGGAGTTGGTGGCGGTGGATCTTTCGATTGATCAGACCCCGTTTAGGGTGGATGAGCTCGAAAGGGTTAAGATTTGTGGTGCAAGAGTGCTTACATTGGATCAGATTGAAGGGCTGAAGAATCCAGATGTCCAGTGTTGGGGCACCGAAGAAGGTGACGATGGTGATCCGCCTAGGCTGTGGGTGCCGAATGGAATGTATCCTGGGACCGCTTTTACCAGGAGTATTGGTGATTCGATTGCTGAGTCAATTGGAGTTGTTGCCAACCCTGAAATTGTGGTTTTGGAGCTTACACAGAATCATCCTTTCTTCGTGCTTGCTAGTGATGGAGTGTTTGAGTTTATTTCTAGCCAAACCGTGGTTGATATGGTTGCAAAATATAAAGATCCACGTGATGCTTGTGCTGCAATAGTTGCCGAATCTTATAAACTCTGGCTGCAGTATGAAACCCGTACAGATGATATTACAGTGATCGTGGTGCATGTAGATGGGCTAACTGCTACTGCTGTTGGTCAATCAGTACATCCTGGTTCTGTTTTACGGCCACCTGTTCCTCAAGTTGTAGAGGTTACAGGATCAGAATCTCCTTCAACCATTAGCTGGAACCCAAGGAACCCTCGCATAAAACATGATTTGTCAAAGGCACGTCTTCGTGTGATTGAAAATTCTCTAGAAAATGGGCAAGTTTGGGTTCCTCCACCTCCAGCCCACAGAAAAACCTGGGAGGAAGAAGCACAAATTGAGCGAGCTTTACATGATCATTTCCTCTTCAGAAAACTTACCGACTCTCAGTGTCATGTTTTATTGGATTGCATGCAAAGGGTTGAGGTCCAGCCTGGAGACATTGTTGTTAGACAGGGTGGTGAAGGTGACTGCTTTTATGTTGTTGGCAGTGGAGAATTTGAGGTGTCGGCAACCCAGGAAGAAAAGAATGGAGAGGTTCCGAGGGTTTTGCAACGATATACAGCTGACAAACTGTCATCCTTTGGAGAACTAGCACTAATGTATAACAAACCACTCCAGGCATCCGTTCGTGCTGTCACCACTGGAACTCTCTGGGCTTTAAGAAGAGAAGACTTTCGTGGAATTCTAATGTCAGAGTTTTCTAATTTGTCATATTTGAAGTTGCTTCGATCAGTGGATCTTTTATCCAGGTTGACGATATTACAGCTCAGCCATATTGCTGATTCTCTTTCTGAGGTTTCATTCTCAGATGGGCAAACAATTGTCAATGAGAATGAAGGCCTTTTGGCATTGTACATTATCCAGAAGGGAAAAGTGAGGATTACAGTTGACGCAAGTTCAGTTAGCAATCCAGTTGTCTGCAATCTCATGTCTGACAATCAAAAACAAGGTGATAATCCTCAGAGCGGTAAAGAGATCATAGTGGAGAAGACAGAGGGAAGCTACTTTGGCGAATGGACACTTCTCGGGGAACATATTGATTTGTTTAATGCAGTTGCTGTGGGGGATGTTGTTTGTGCTGTTTTGACAAAGGAAAATTTTGATTCAGTCATTGGCCCAATGACAAAGCTTACTCAGGATGATCAGAAATCAAGGGATCAATCTTCGGAAACTTTAATGGAATCTGTTAAAAGTATTGATATCTCTGCTCTTACTAAAGTTCAGCTTGCTGATATGGAGTGGAGAAAGTGTCTGTATTCTACTGACTGCAGTGAGATCGGGCTTGTTCTTTTAAGAGATCCAGAAAATTTGCTTAGTTTGAAAAGGTTTTCAAAGAAGAAGGTCAGAAAGCTTGGAAAGGAAGCACAAGTATTAAAAGAGAAGGATCTAATCAAGAGCATAAGCCCTTCAGCTTGTGTGCCACAGGTTCTATCCACTTGTGTTGATCAGACACATGCTGCAATACTGCTCAATACATGCATTGCTTGTCCATTGGCCTCGATACTTCGCACGCCCCTTGATGAACCATCTGCCCGATTTTGTACAGCCTCTCTAATTACTGCTTTGGAAGATCTCCACAAGAATGACGTTCTCTACAGAGGCTTGTCTCCTGATGCATTAATGTTGGACCACACGGGACATTTACAGCTTGTAGACTTCAGATTTGGGAAAAAGTTGTCTGGCCAAAGAACATATACAATTTGTGGAATGGCAGACTTTTTAGCTCCAGAGGTAGTTCAGGGAATAGGCCATGGTTTCCCAGCTGACTGGTGGGCATTGGGAGTCTTGATATATTTCATGTTACAAGGTGAATTGCCTTTTGGGTCATGGAGAGAAAGTGAGCTTGATATCTTTGCAAAGATTGCGAAGGGACAGCTTAAACTTCCTCAAACTTTCAGCCCTGGAGTTGTTGATCTCATTACCAAGTTACTTGAGGTTGATGAAAACACCAGACTAGGAAGCCAAGGTTGTGATTCTGTTAAAAGTCATCCCTGGTTCGATGGTATTGATTGGAAAGGGATTAGAGACTCTAGTTTTCCTGTTCCTCAGGAGATCACCTCTCGCATAGCTCAACATTTGGAAAGTCACTCAGACGAGTATAGTGCTCCTCAAGGTTCTCCGTCACACAATGAAGAAGAACTTGACATTCCGGAATGGTTTGATGACTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

1077

Amino Acids

119.62

Weight (kDa)

5.04

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 116 - 359 7.6e-36 Protein phosphatase 2C
cNMP_binding PF00027 494 - 585 8.7e-16 Cyclic nucleotide-binding domain
Pkinase PF00069 786 - 1020 2.5e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 807 - 1006 2.6e-09 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1083
AccB1I GGYRCC 2 cut(s) 800, 841
AccB7I CCANNNNNTGG 2 cut(s) 1015, 1257
AccBSI CCGCTC 1 cut(s) 2028
AccI GTMKAC 1 cut(s) 2715
AccII CGCG 1 cut(s) 501
AccIII TCCGGA 1 cut(s) 3211
AciI CCGC 5 cut(s) 479, 671, 827, 867, 2028
AcoI YGGCCR 3 cut(s) 394, 1199, 2743
AcsI RAATTY 6 cut(s) 451, 1321, 1556, 1743, 2158, 2368
AcuI CTGAAG 4 cut(s) 794, 1411, 2454, 2704
AcvI CACGTG 1 cut(s) 1049
AfaI GTAC 5 cut(s) 272, 1111, 1179, 1916, 2605
AfiI CCNNNNNNNGG 9 cut(s) 224, 291, 621, 702, 703, 796, 1015, 1257, 2027
AhdI GACNNNNNGTC 1 cut(s) 1633
AjiI CACGTC 1 cut(s) 1304
AjuI GAANNNNNNNTTGG 2 cut(s) 2865, 2897
Alw21I GWGCWC 2 cut(s) 717, 3169
Alw26I GTCTC 3 cut(s) 1491, 2673, 3081
AlwNI CAGNNNCTG 1 cut(s) 2120
Ama87I CYCGRG 1 cut(s) 2087
Aor13HI TCCGGA 1 cut(s) 3211
AoxI GGCC 8 cut(s) 69, 394, 1199, 1900, 2176, 2556, 2743, 2809
ApeKI GCWGC 3 cut(s) 1061, 1093, 2519
ApoI RAATTY 6 cut(s) 451, 1321, 1556, 1743, 2158, 2368
ArsI GACNNNNNNTTYG 4 cut(s) 362, 394, 2708, 2740
AseI ATTAAT 1 cut(s) 2681
Asp700I GAANNNNTTC 1 cut(s) 1734
AspA2I CCTAGG 1 cut(s) 830
AspS9I GGNCC 6 cut(s) 70, 228, 864, 1486, 2177, 2689
AsuC2I CCSGG 1 cut(s) 365
AvaI CYCGRG 1 cut(s) 2087
AvaII GGWCC 4 cut(s) 228, 864, 1486, 2689
AvrII CCTAGG 1 cut(s) 830
AxyI CCTNAGG 1 cut(s) 3108
BaeGI GKGCMC 1 cut(s) 803
BalI TGGCCA 2 cut(s) 396, 2745
BanI GGYRCC 2 cut(s) 800, 841
BanII GRGCYC 1 cut(s) 717
BbrPI CACGTG 1 cut(s) 1049
BbsI GAAGAC 3 cut(s) 1298, 1737, 2057
Bbv12I GWGCWC 2 cut(s) 717, 3169
BbvI GCAGC 3 cut(s) 1048, 1080, 2506
BccI CCATC 8 cut(s) 125, 386, 812, 977, 1139, 1865, 2596, 3056
BceAI ACGGC 1 cut(s) 1214
BcgI CGANNNNNNTGC 6 cut(s) 1591, 1625, 2320, 2354, 3146, 3180
BciVI GTATCC 1 cut(s) 867
BclI TGATCA 5 cut(s) 136, 688, 1414, 2203, 2506
BcnI CCSGG 1 cut(s) 365
BcoDI GTCTC 3 cut(s) 1491, 2673, 3081
BfaI CTAG 9 cut(s) 186, 630, 831, 978, 1005, 1328, 1652, 3017, 3093
BfmI CTRYAG 3 cut(s) 1094, 2329, 2656
BfuI GTATCC 1 cut(s) 867
BglII AGATCT 1 cut(s) 2632
BisI GCNGC 3 cut(s) 1062, 1094, 2520
BlnI CCTAGG 1 cut(s) 830
BlpI GCTNAGC 1 cut(s) 1829
BlsI GCNGC 3 cut(s) 1063, 1095, 2521
Bme18I GGWCC 4 cut(s) 228, 864, 1486, 2689
BmeRI GACNNNNNGTC 1 cut(s) 1633
BmeT110I CYCGRG 1 cut(s) 2087
BmgBI CACGTC 1 cut(s) 1304
BmgT120I GGNCC 6 cut(s) 70, 228, 864, 1486, 2177, 2689
BmrI ACTGGG 1 cut(s) 92
BmsI GCATC 8 cut(s) 43, 157, 295, 348, 472, 1042, 1691, 2665
BmuI ACTGGG 1 cut(s) 92
BpiI GAAGAC 3 cut(s) 1298, 1737, 2057
BplI GAGNNNNNCTC 2 cut(s) 3104, 3136
BpmI CTGGAG 5 cut(s) 1344, 1515, 1661, 2772, 2985
Bpu10I CCTNAGC 1 cut(s) 458
Bpu1102I GCTNAGC 1 cut(s) 1829
BpuEI CTTGAG 3 cut(s) 1197, 3014, 3156
BpuMI CCSGG 1 cut(s) 365
BsaAI YACGTR 1 cut(s) 1049
BsaBI GATNNNNATC 3 cut(s) 135, 822, 1237
BsaWI WCCGGW 1 cut(s) 3211
BsaXI ACNNNNNCTCC 8 cut(s) 871, 901, 1488, 1518, 2296, 2326, 2884, 2914
Bsc4I CCNNNNNNNGG 9 cut(s) 224, 291, 621, 702, 703, 796, 1015, 1257, 2027
Bse1I ACTGG 6 cut(s) 98, 790, 1708, 1973, 2834, 3233
Bse21I CCTNAGG 1 cut(s) 3108
Bse3DI GCAATG 2 cut(s) 609, 2541
Bse8I GATNNNNATC 3 cut(s) 135, 822, 1237
BseAI TCCGGA 1 cut(s) 3211
BseJI GATNNNNATC 3 cut(s) 135, 822, 1237
BseLI CCNNNNNNNGG 9 cut(s) 224, 291, 621, 702, 703, 796, 1015, 1257, 2027
BseMI GCAATG 2 cut(s) 609, 2541
BseNI ACTGG 6 cut(s) 98, 790, 1708, 1973, 2834, 3233
BseRI GAGGAG 2 cut(s) 674, 3159
BseSI GKGCMC 1 cut(s) 803
BseXI GCAGC 3 cut(s) 1048, 1080, 2506
BseYI CCCAGC 1 cut(s) 2821
Bsh1236I CGCG 1 cut(s) 501
BshFI GGCC 8 cut(s) 71, 396, 1201, 1902, 2178, 2558, 2745, 2811
BshNI GGYRCC 2 cut(s) 800, 841
BsiHKAI GWGCWC 2 cut(s) 717, 3169
BsiHKCI CYCGRG 1 cut(s) 2087
BsiSI CCGG 3 cut(s) 252, 365, 3212
BslFI GGGAC 5 cut(s) 268, 370, 877, 2712, 2946
BslI CCNNNNNNNGG 9 cut(s) 224, 291, 621, 702, 703, 796, 1015, 1257, 2027
BsmAI GTCTC 3 cut(s) 1491, 2673, 3081
BsmFI GGGAC 5 cut(s) 268, 370, 877, 2712, 2946
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 8 cut(s) 71, 396, 1201, 1902, 2178, 2558, 2745, 2811
BsoBI CYCGRG 1 cut(s) 2087
Bsp1286I GDGCHC 3 cut(s) 717, 803, 3169
Bsp13I TCCGGA 1 cut(s) 3211
Bsp1407I TGTACA 2 cut(s) 1914, 2603
Bsp1720I GCTNAGC 1 cut(s) 1829
Bsp19I CCATGG 2 cut(s) 397, 2812
Bsp68I TCGCGA 1 cut(s) 501
BspACI CCGC 5 cut(s) 479, 671, 827, 867, 2028
BspANI GGCC 8 cut(s) 71, 396, 1201, 1902, 2178, 2558, 2745, 2811
BspEI TCCGGA 1 cut(s) 3211
BspFNI CGCG 1 cut(s) 501
BspMAI CTGCAG 2 cut(s) 1098, 2333
BspT107I GGYRCC 2 cut(s) 800, 841
BsrBI CCGCTC 1 cut(s) 2028
BsrDI GCAATG 2 cut(s) 609, 2541
BsrGI TGTACA 2 cut(s) 1914, 2603
BsrI ACTGG 6 cut(s) 98, 790, 1708, 1973, 2834, 3233
BssT1I CCWWGG 5 cut(s) 397, 830, 1265, 2812, 3025
Bst4CI ACNGT 7 cut(s) 210, 238, 583, 1015, 1126, 1635, 1948
Bst6I CTCTTC 3 cut(s) 121, 1430, 1720
BstAPI GCANNNNNTGC 2 cut(s) 1070, 2924
BstAUI TGTACA 2 cut(s) 1914, 2603
BstBAI YACGTR 1 cut(s) 1049
BstC8I GCNNGC 5 cut(s) 975, 1404, 1472, 2289, 2573
BstDSI CCRYGG 3 cut(s) 397, 1014, 2812
BstFNI CGCG 1 cut(s) 501
BstMAI GTCTC 3 cut(s) 1491, 2673, 3081
BstMWI GCNNNNNNNGC 9 cut(s) 77, 491, 512, 641, 841, 1061, 1070, 2070, 2924
BstNSI RCATGY 4 cut(s) 1142, 1474, 2519, 2541
BstSFI CTRYAG 3 cut(s) 1094, 2329, 2656
BstSLI GKGCMC 1 cut(s) 803
BstUI CGCG 1 cut(s) 501
BstV1I GCAGC 3 cut(s) 1048, 1080, 2506
BstV2I GAAGAC 3 cut(s) 1298, 1737, 2057
BstX2I RGATCY 7 cut(s) 93, 676, 1042, 1798, 2359, 2446, 2632
BstXI CCANNNNNNTGG 1 cut(s) 2829
BstYI RGATCY 7 cut(s) 93, 676, 1042, 1798, 2359, 2446, 2632
Bsu36I CCTNAGG 1 cut(s) 3108
BsuI GTATCC 1 cut(s) 867
BsuRI GGCC 8 cut(s) 71, 396, 1201, 1902, 2178, 2558, 2745, 2811
BtgI CCRYGG 3 cut(s) 397, 1014, 2812
BtrI CACGTC 1 cut(s) 1304
BtsI GCAGTG 2 cut(s) 1555, 2338
BtsIMutI CAGTG 8 cut(s) 87, 797, 1131, 1454, 1555, 1701, 1800, 2338
BtuMI TCGCGA 1 cut(s) 501
Cac8I GCNNGC 5 cut(s) 975, 1404, 1472, 2289, 2573
CaiI CAGNNNCTG 1 cut(s) 2120
Cfr13I GGNCC 6 cut(s) 70, 228, 864, 1486, 2177, 2689
CseI GACGC 1 cut(s) 1961
Csp6I GTAC 5 cut(s) 271, 1110, 1178, 1915, 2604
CspCI CAANNNNNGTGG 2 cut(s) 2485, 2520
CviQI GTAC 5 cut(s) 271, 1110, 1178, 1915, 2604
DriI GACNNNNNGTC 1 cut(s) 1633
EaeI YGGCCR 3 cut(s) 394, 1199, 2743
Eam1104I CTCTTC 3 cut(s) 121, 1430, 1720
Eam1105I GACNNNNNGTC 1 cut(s) 1633
EarI CTCTTC 3 cut(s) 121, 1430, 1720
EciI GGCGGA 1 cut(s) 816
Ecl136II GAGCTC 1 cut(s) 715
Eco130I CCWWGG 5 cut(s) 397, 830, 1265, 2812, 3025
Eco147I AGGCCT 1 cut(s) 1902
Eco24I GRGCYC 1 cut(s) 717
Eco32I GATATC 2 cut(s) 2263, 2911
Eco47I GGWCC 4 cut(s) 228, 864, 1486, 2689
Eco53kI GAGCTC 1 cut(s) 715
Eco57I CTGAAG 4 cut(s) 794, 1411, 2454, 2704
Eco72I CACGTG 1 cut(s) 1049
Eco81I CCTNAGG 1 cut(s) 3108
Eco88I CYCGRG 1 cut(s) 2087
EcoICRI GAGCTC 1 cut(s) 715
EcoRI GAATTC 1 cut(s) 1743
EcoRV GATATC 2 cut(s) 2263, 2911
EcoT14I CCWWGG 5 cut(s) 397, 830, 1265, 2812, 3025
EcoT22I ATGCAT 2 cut(s) 2543, 2680
EcoT38I GRGCYC 1 cut(s) 717
ErhI CCWWGG 5 cut(s) 397, 830, 1265, 2812, 3025
FalI AAGNNNNNCTT 4 cut(s) 2271, 2303, 3186, 3218
FaqI GGGAC 5 cut(s) 268, 370, 877, 2712, 2946
FbaI TGATCA 5 cut(s) 136, 688, 1414, 2203, 2506
FblI GTMKAC 1 cut(s) 2715
Fnu4HI GCNGC 3 cut(s) 1062, 1094, 2520
FriOI GRGCYC 1 cut(s) 717
Fsp4HI GCNGC 3 cut(s) 1062, 1094, 2520
FspBI CTAG 9 cut(s) 186, 630, 831, 978, 1005, 1328, 1652, 3017, 3093
GluI GCNGC 3 cut(s) 1062, 1094, 2520
GsaI CCCAGC 1 cut(s) 2825
GsuI CTGGAG 5 cut(s) 1344, 1515, 1661, 2772, 2985
HaeIII GGCC 8 cut(s) 71, 396, 1201, 1902, 2178, 2558, 2745, 2811
HapII CCGG 3 cut(s) 252, 365, 3212
HgaI GACGC 1 cut(s) 1961
HincII GTYRAC 2 cut(s) 1815, 1951
HindII GTYRAC 2 cut(s) 1815, 1951
HindIII AAGCTT 2 cut(s) 2189, 2411
HpaII CCGG 3 cut(s) 252, 365, 3212
Hpy166II GTNNAC 3 cut(s) 1815, 1951, 2716
Hpy8I GTNNAC 3 cut(s) 1815, 1951, 2716
HpyCH4III ACNGT 7 cut(s) 210, 238, 583, 1015, 1126, 1635, 1948
HpyCH4IV ACGT 3 cut(s) 1048, 1303, 2649
HpyF10VI GCNNNNNNNGC 9 cut(s) 77, 491, 512, 641, 841, 1061, 1070, 2070, 2924
HpySE526I ACGT 3 cut(s) 1048, 1303, 2649
Kpn2I TCCGGA 1 cut(s) 3211
Ksp22I TGATCA 5 cut(s) 136, 688, 1414, 2203, 2506
LmnI GCTCC 3 cut(s) 943, 2791, 3172
Lsp1109I GCAGC 3 cut(s) 1048, 1080, 2506
LweI GCATC 8 cut(s) 43, 157, 295, 348, 472, 1042, 1691, 2665
MaeI CTAG 9 cut(s) 186, 630, 831, 978, 1005, 1328, 1652, 3017, 3093
MaeII ACGT 3 cut(s) 1048, 1303, 2649
MaeIII GTNAC 8 cut(s) 812, 1225, 1526, 1696, 2862, 2988, 3146, 3183
MbiI CCGCTC 1 cut(s) 2028
MfeI CAATTG 2 cut(s) 906, 1881
MflI RGATCY 7 cut(s) 93, 676, 1042, 1798, 2359, 2446, 2632
MhlI GDGCHC 3 cut(s) 717, 803, 3169
MlsI TGGCCA 2 cut(s) 396, 2745
MluNI TGGCCA 2 cut(s) 396, 2745
MlyI GAGTC 5 cut(s) 107, 911, 1436, 2853, 3083
MmeI TCCRAC 1 cut(s) 2667
Mox20I TGGCCA 2 cut(s) 396, 2745
Mph1103I ATGCAT 2 cut(s) 2543, 2680
MroI TCCGGA 1 cut(s) 3211
MroXI GAANNNNTTC 1 cut(s) 1734
MscI TGGCCA 2 cut(s) 396, 2745
MslI CAYNNNNRTG 2 cut(s) 1143, 2643
Msp20I TGGCCA 2 cut(s) 396, 2745
MspA1I CMGCKG 2 cut(s) 1625, 2825
MspI CCGG 3 cut(s) 252, 365, 3212
MunI CAATTG 2 cut(s) 906, 1881
Mva1269I GAATGC 1 cut(s) 82
MvnI CGCG 1 cut(s) 501
MwoI GCNNNNNNNGC 9 cut(s) 77, 491, 512, 641, 841, 1061, 1070, 2070, 2924
NciI CCSGG 1 cut(s) 365
NcoI CCATGG 2 cut(s) 397, 2812
NmeAIII GCCGAG 1 cut(s) 670
NmuCI GTSAC 5 cut(s) 812, 1526, 1696, 3146, 3183
NruI TCGCGA 1 cut(s) 501
NsiI ATGCAT 2 cut(s) 2543, 2680
NspI RCATGY 4 cut(s) 1142, 1474, 2519, 2541
PaeI GCATGC 1 cut(s) 1474
PceI AGGCCT 1 cut(s) 1902
PctI GAATGC 1 cut(s) 82
PdmI GAANNNNTTC 1 cut(s) 1734
PflMI CCANNNNNTGG 2 cut(s) 1015, 1257
PkrI GCNGC 3 cut(s) 1063, 1095, 2521
PleI GAGTC 5 cut(s) 107, 910, 1436, 2852, 3083
PmaCI CACGTG 1 cut(s) 1049
PmlI CACGTG 1 cut(s) 1049
PpsI GAGTC 5 cut(s) 107, 910, 1436, 2852, 3083
Ppu21I YACGTR 1 cut(s) 1049
PshBI ATTAAT 1 cut(s) 2681
PsiI TTATAA 1 cut(s) 1083
Psp124BI GAGCTC 1 cut(s) 717
PspCI CACGTG 1 cut(s) 1049
PspFI CCCAGC 1 cut(s) 2821
PspPI GGNCC 6 cut(s) 70, 228, 864, 1486, 2177, 2689
PstI CTGCAG 2 cut(s) 1098, 2333
PstNI CAGNNNCTG 1 cut(s) 2120
PsuI RGATCY 7 cut(s) 93, 676, 1042, 1798, 2359, 2446, 2632
PvuII CAGCTG 2 cut(s) 1625, 2825
RruI TCGCGA 1 cut(s) 501
RsaI GTAC 5 cut(s) 272, 1111, 1179, 1916, 2605
RsaNI GTAC 5 cut(s) 271, 1110, 1178, 1915, 2604
RseI CAYNNNNRTG 2 cut(s) 1143, 2643
SacI GAGCTC 1 cut(s) 717
SatI GCNGC 3 cut(s) 1062, 1094, 2520
Sau96I GGNCC 6 cut(s) 70, 228, 864, 1486, 2177, 2689
SchI GAGTC 5 cut(s) 107, 911, 1436, 2853, 3083
SduI GDGCHC 3 cut(s) 717, 803, 3169
SfaNI GCATC 8 cut(s) 43, 157, 295, 348, 472, 1042, 1691, 2665
SfcI CTRYAG 3 cut(s) 1094, 2329, 2656
SinI GGWCC 4 cut(s) 228, 864, 1486, 2689
SmiMI CAYNNNNRTG 2 cut(s) 1143, 2643
SmlI CTYRAG 3 cut(s) 1212, 2993, 3171
SmoI CTYRAG 3 cut(s) 1212, 2993, 3171
SphI GCATGC 1 cut(s) 1474
SseBI AGGCCT 1 cut(s) 1902
SsiI CCGC 5 cut(s) 479, 671, 827, 867, 2028
SspMI CTAG 9 cut(s) 186, 630, 831, 978, 1005, 1328, 1652, 3017, 3093
SstI GAGCTC 1 cut(s) 717
StuI AGGCCT 1 cut(s) 1902
StyI CCWWGG 5 cut(s) 397, 830, 1265, 2812, 3025
TaaI ACNGT 7 cut(s) 210, 238, 583, 1015, 1126, 1635, 1948
TaiI ACGT 3 cut(s) 1051, 1306, 2652
TatI WGTACW 3 cut(s) 1177, 1914, 2603
TscAI CASTG 8 cut(s) 94, 797, 1131, 1454, 1555, 1708, 1800, 2338
TseFI GTSAC 5 cut(s) 812, 1526, 1696, 3146, 3183
TseI GCWGC 3 cut(s) 1061, 1093, 2519
Tsp45I GTSAC 5 cut(s) 812, 1526, 1696, 3146, 3183
TspDTI ATGAA 7 cut(s) 1116, 1851, 1911, 2598, 2848, 3018, 3207
TspGWI ACGGA 3 cut(s) 339, 1675, 3171
TspRI CASTG 8 cut(s) 94, 797, 1131, 1454, 1555, 1708, 1800, 2338
Van91I CCANNNNNTGG 2 cut(s) 1015, 1257
VpaK11BI GGWCC 4 cut(s) 228, 864, 1486, 2689
VspI ATTAAT 1 cut(s) 2681
XapI RAATTY 6 cut(s) 451, 1321, 1556, 1743, 2158, 2368
XbaI TCTAGA 1 cut(s) 1327
XceI RCATGY 4 cut(s) 1142, 1474, 2519, 2541
XcmI CCANNNNNNNNNTGG 4 cut(s) 350, 404, 583, 2829
XmaJI CCTAGG 1 cut(s) 830
XmiI GTMKAC 1 cut(s) 2715
XmnI GAANNNNTTC 1 cut(s) 1734
XspI CTAG 9 cut(s) 186, 630, 831, 978, 1005, 1328, 1652, 3017, 3093
Zsp2I ATGCAT 2 cut(s) 2543, 2680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.