Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0011815

Physical Location & Seq

Genomic Coordinates Reverse (-)

10238593 .. 10245673

Gene Structure 7081 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1126400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2373 bp

ATGATCTCGGACGGAGTTGAAGATGAAGAGAAATGGCTGGCAGCTGGAATCGCCGGCCTCCAACAGAACGCCTTCTATATGCACCGAGCTCTGGACTCGAACAATCTCAGAGACGCGCTCAAGTACTCAGCTCAGATGCTGTCGGAGCTCCGGACCTCGAAGCTTTCCCCTCACAAGTACTACGAACTCTATATGCGGGCGTTTGACGAATTGAGGAAATTGGAAATGTTCTTCAAGGAGGAGGCTCGGCGCGGCTGCTCCATTATCGATCTCTATGAGCTTGTTCAGCACGCCGGCAACATATTGCCTAGATTGTATCTTCTCTGTACAGTAGGTTCTGTGTACATCAAATCTAAGGAAGCTCCTGCTAAGGATGTCCTAAAAGATCTCGTTGAAATGTCCCGTGGCATTCAGAATCCTGTACGTGGGCTGTTCTTAAGGAGTTACCTTTCTCAAGTGAGTAGGGACAAATTGCCTGATATTGGTTCTGAATATGAAGGAGATGCCGACACTGTCAAGGACGCTGTTGAGTTTGTGCTCCAAAATTTCACTGAGATGAACAAACTTTGGGTGCGGATGCAACATCAGGGTCCTGCCCGGGAAAAGGAGAAACGGGAGAAAGAAAGAAGCGAGCTACGTGATCTTGTTGGTAAAAACTTGCACGTCCTAAGCCAGATAGAAGGTGTTGATTTGGATTTGTACAAAGAGACCGTGCTTCCCAGAGTCCTGGAGCAGGTTGTCAATTGTAAGGATGAGATTGCACAGTTTTATTTGATGGATTGCATAATTCAAGTCTTTCCCGATGAGTACCACTTGCAAACTCTTGACGTATTGTTGGGTGCTTGCCCCCAACTTCAGCCATCTGTTGATATTAAGACAGTTCTATCCCAGTTAATGGAAAGACTTTCAAATTATGCTGCTTCAAGTACAGAAGTATTGCCTGAGTTTTTACAAGTACAAGCTTTTTCAAAACTGAGCAATGCTATTGGAAAGGTGATTGAAGCCCAAGTTGACATGCCTATCATCGGTGTTGTCACTCTATATTCATCTCTTCTTAAATTCACCCTCCATGTGCACCCTGATCGACTTGATTATGCTGATCAAGTGTTGGGCTCATTTGTTAAGAAACTCTCTGGAAAAGGGAAAATTCAAGACAGCAGGGCGACAAAACAAGTGGTTGCACTTTTAAGTGCTCCACTTGAGAAATATAATGATATTGTCACGGCTTTGAAGCTTACAAATTATCCTCGCGTCCTGGAATTCCTTGATAGTGGAACAAATAAAGTCATGGCTACTGTTATAATTCAGAGCATTATGAAAAATACAACTCATGTTTTAACTGCTGAGAAGGTCGAGGCTTTGTTTGAATTAATAAAAGGACTTATTGAGGATTTGGATGGGACTCTTAATGATGAGGTTGATGAAGAAGATTTCAAGGAGGAGCAAAATTCAGTTGCACGACTTATTCAAATGTTTTCTAATGATGATCCTGAAGAGATGTTTAAGATCATATGTACTGTGAAGAAGCACATCCTGGTTGGAGGACCGACGCGTTTGCCCTTTACTGTCCCTCCCCTAGTTTTCTCTTCTCTAAAGTTGGTTAGGAAATTGCAAGCCCAGGACGAGAATCCATTTGGAGATGATGCATCAACCACACCAAAGAAAATTTTTCAGCTCTTGACTCAGACTATTGAGGCTTTGTTGAATGTTCCAGCACCTGAACTGGCATTACGGTTATATTTGCAATGTGCTGAGACTGCAAATGACTCTGATTTGGAACCCGTTGCCTATGAATTCTTCACCCAAGCATATATTCTTTACGAAGAAGAAATTTCAGATTCGAAAGCACAGGTGACTGCCATTCATCTGATAATTGGGACTCTCCAGAGGATGCATGTATTTGGCGTTGAGAACAGGGATACTTTAACTCACAAAGCCACAGGGTATTCGGCAAAGCTTTTAAAGAAGGCTGATCAGTGCCGAGCTATATATGCTTGCTCGCACCTCTTCTGGGTCGACAATCAGGAGAGCATGAAGGATGGAGAAAGGGTACTGATTTGCCTAAAGCGAGCTTTACGAATCGCTAACGCTGCCCAACAGATGTCCAATGCTACACGGGGTAGCACTGGTTCAGTAACGCTCTTTGTTGAAATACTGAACAAGTACCTCTATTTCTTCGAGAAGGGGAACCCACAGATAACAGTTGCTTCAGTCCAAAGCCTGATTGAATTGGTCACAACTGAACTGCAAAGCGACTCGGCCTCAGCAGAGCCATCTACAGATGCTTTCTTTAAAAGCACACTGCGGTACATTCAGTTCCAGAAGCAGAAAGGTGGTGCAGTTGGTGAAAGATATGAGTCCATCAAGGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008565 GO:0010008 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0016197 GO:0016482 GO:0030904 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042147 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

Vps35

Protein Analysis

791

Amino Acids

89.25

Weight (kDa)

5.41

Isoelectric Point (pI)

42.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps35	PF03635	12 - 747	1.3e-287	Vacuolar protein sorting-associated protein 35

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011815)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17790 AT3G51310
fragaria_vesca	FvH4_1g11410
malus_domestica	MD02G1126400.v1.1 MD15G1242400.v1.1
prunus_persica	Prupe.7G171800_v2.0.a1 Prupe.7G171800_v2.0.a1
pyrus_communis	pycom02g09870 pycom15g21360
rosa_chinensis	RchiOBHm_Chr2g0098981
rosa_laevigata	RLG00000016838
rosa_multiflora	Rmu_sc0000577.1_g000009
rosa_roxburghii	Rroxscaffold_2G00143530
rosa_rugosa	Rorug02G0075400
rosa_samantha	Rh2AG124900 Rh2BG128100 Rh2CG129000 Rh2DG129200
rosa_wichuraiana	Rw2G009590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1301
Acc36I	ACCTGC	1 cut(s)	724
AccB7I	CCANNNNNTGG	1 cut(s)	895
AccI	GTMKAC	1 cut(s)	2016
AccII	CGCG	4 cut(s)	116, 252, 1251, 1552
AccIII	TCCGGA	1 cut(s)	150
AciI	CCGC	4 cut(s)	196, 252, 574, 2305
AclWI	GGATC	1 cut(s)	1481
AcsI	RAATTY	8 cut(s)	544, 1058, 1146, 1259, 1447, 1665, 1793, 1830
AcuI	CTGAAG	3 cut(s)	839, 1512, 2193
AfiI	CCNNNNNNNGG	8 cut(s)	91, 425, 482, 604, 733, 895, 1025, 2011
AflII	CTTAAG	1 cut(s)	436
AflIII	ACRYGT	1 cut(s)	1550
AjiI	CACGTC	1 cut(s)	664
AjnI	CCWGG	4 cut(s)	726, 1254, 1533, 1617
AjuI	GAANNNNNNNTTGG	2 cut(s)	1797, 1829
Alw21I	GWGCWC	5 cut(s)	91, 150, 540, 1077, 1195
Alw26I	GTCTC	3 cut(s)	105, 701, 1748
Alw44I	GTGCAC	1 cut(s)	1073
AlwI	GGATC	1 cut(s)	1481
AlwNI	CAGNNNCTG	2 cut(s)	139, 1718
Ama87I	CYCGRG	1 cut(s)	597
Aor13HI	TCCGGA	1 cut(s)	150
AoxI	GGCC	2 cut(s)	55, 2259
ApaLI	GTGCAC	1 cut(s)	1073
ApeKI	GCWGC	4 cut(s)	41, 255, 917, 2090
ApoI	RAATTY	8 cut(s)	544, 1058, 1146, 1259, 1447, 1665, 1793, 1830
AseI	ATTAAT	1 cut(s)	1370
Asp700I	GAANNNNTTC	1 cut(s)	71
AspLEI	GCGC	2 cut(s)	118, 252
AspS9I	GGNCC	3 cut(s)	153, 590, 1544
AsuC2I	CCSGG	2 cut(s)	598, 599
AsuHPI	GGTGA	5 cut(s)	1006, 1054, 1792, 1864, 2357
AsuII	TTCGAA	1 cut(s)	1841
AvaI	CYCGRG	1 cut(s)	597
AvaII	GGWCC	3 cut(s)	153, 590, 1544
BaeGI	GKGCMC	1 cut(s)	1077
BanII	GRGCYC	3 cut(s)	91, 150, 1115
Bbv12I	GWGCWC	5 cut(s)	91, 150, 540, 1077, 1195
BbvCI	CCTCAGC	1 cut(s)	2263
BbvI	GCAGC	4 cut(s)	53, 242, 904, 2077
BccI	CCATC	6 cut(s)	769, 868, 1391, 2033, 2281, 2369
BceAI	ACGGC	1 cut(s)	1239
BcgI	CGANNNNNNTGC	4 cut(s)	1064, 1098, 1537, 1571
BciT130I	CCWGG	4 cut(s)	728, 1256, 1535, 1619
BciVI	GTATCC	1 cut(s)	1912
BclI	TGATCA	2 cut(s)	1099, 1972
BcnI	CCSGG	2 cut(s)	598, 599
BcoDI	GTCTC	3 cut(s)	105, 701, 1748
BfaI	CTAG	2 cut(s)	309, 1577
BfmI	CTRYAG	1 cut(s)	2277
BfrI	CTTAAG	1 cut(s)	436
BfuAI	ACCTGC	1 cut(s)	724
BfuI	GTATCC	1 cut(s)	1912
BglII	AGATCT	1 cut(s)	385
BisI	GCNGC	5 cut(s)	42, 253, 256, 918, 2091
BlsI	GCNGC	5 cut(s)	43, 254, 257, 919, 2092
BmcAI	AGTACT	2 cut(s)	125, 179
Bme1390I	CCNGG	6 cut(s)	598, 599, 728, 1256, 1535, 1619
Bme18I	GGWCC	3 cut(s)	153, 590, 1544
BmeT110I	CYCGRG	1 cut(s)	597
BmgBI	CACGTC	1 cut(s)	664
BmgT120I	GGNCC	3 cut(s)	153, 590, 1544
BmiI	GGNNCC	3 cut(s)	591, 1779, 2189
BmrFI	CCNGG	6 cut(s)	598, 599, 728, 1256, 1535, 1619
BmrI	ACTGGG	1 cut(s)	883
BmsI	GCATC	7 cut(s)	126, 493, 567, 1633, 1655, 1881, 2272
BmuI	ACTGGG	1 cut(s)	883
BplI	GAGNNNNNCTC	2 cut(s)	102, 134
BpmI	CTGGAG	2 cut(s)	749, 1868
Bpu10I	CCTNAGC	3 cut(s)	369, 668, 2263
Bpu14I	TTCGAA	1 cut(s)	1841
BpuEI	CTTGAG	3 cut(s)	104, 438, 1220
BpuMI	CCSGG	2 cut(s)	598, 599
Bsa29I	ATCGAT	1 cut(s)	267
BsaAI	YACGTR	2 cut(s)	425, 638
BsaI	GGTCTC	1 cut(s)	701
BsaJI	CCNNGG	3 cut(s)	403, 597, 1617
BsaWI	WCCGGW	1 cut(s)	150
BsaXI	ACNNNNNCTCC	4 cut(s)	1555, 1585, 2034, 2064
Bsc4I	CCNNNNNNNGG	8 cut(s)	91, 425, 482, 604, 733, 895, 1025, 2011
Bse118I	RCCGGY	2 cut(s)	53, 293
Bse1I	ACTGG	3 cut(s)	889, 1728, 2131
Bse3DI	GCAATG	2 cut(s)	985, 1751
BseAI	TCCGGA	1 cut(s)	150
BseBI	CCWGG	4 cut(s)	728, 1256, 1535, 1619
BseCI	ATCGAT	1 cut(s)	267
BseDI	CCNNGG	3 cut(s)	403, 597, 1617
BseGI	GGATG	7 cut(s)	379, 582, 757, 1402, 1530, 1896, 2044
BseLI	CCNNNNNNNGG	8 cut(s)	91, 425, 482, 604, 733, 895, 1025, 2011
BseMI	GCAATG	2 cut(s)	985, 1751
BseNI	ACTGG	3 cut(s)	889, 1728, 2131
BseRI	GAGGAG	2 cut(s)	254, 1454
BseSI	GKGCMC	1 cut(s)	1077
BseXI	GCAGC	4 cut(s)	53, 242, 904, 2077
BsgI	GTGCAG	1 cut(s)	2358
Bsh1236I	CGCG	4 cut(s)	116, 252, 1251, 1552
BshFI	GGCC	2 cut(s)	57, 2261
BshVI	ATCGAT	1 cut(s)	267
BsiHKAI	GWGCWC	5 cut(s)	91, 150, 540, 1077, 1195
BsiHKCI	CYCGRG	1 cut(s)	597
BsiSI	CCGG	4 cut(s)	54, 151, 294, 598
BslFI	GGGAC	5 cut(s)	385, 479, 1414, 1553, 1891
BslI	CCNNNNNNNGG	8 cut(s)	91, 425, 482, 604, 733, 895, 1025, 2011
BsmAI	GTCTC	3 cut(s)	105, 701, 1748
BsmBI	CGTCTC	1 cut(s)	105
BsmFI	GGGAC	5 cut(s)	385, 479, 1414, 1553, 1891
BsmI	GAATGC	1 cut(s)	408
BsnI	GGCC	2 cut(s)	57, 2261
Bso31I	GGTCTC	1 cut(s)	701
BsoBI	CYCGRG	1 cut(s)	597
Bsp119I	TTCGAA	1 cut(s)	1841
Bsp1286I	GDGCHC	6 cut(s)	91, 150, 540, 1077, 1115, 1195
Bsp13I	TCCGGA	1 cut(s)	150
Bsp1407I	TGTACA	3 cut(s)	326, 342, 699
Bsp143I	GATC	9 cut(s)	3, 268, 385, 640, 1081, 1099, 1486, 1506, 1972
BspACI	CCGC	4 cut(s)	196, 252, 574, 2305
BspANI	GGCC	2 cut(s)	57, 2261
BspDI	ATCGAT	1 cut(s)	267
BspEI	TCCGGA	1 cut(s)	150
BspFNI	CGCG	4 cut(s)	116, 252, 1251, 1552
BspLI	GGNNCC	3 cut(s)	591, 1779, 2189
BspMI	ACCTGC	1 cut(s)	724
BspPI	GGATC	1 cut(s)	1481
BspT104I	TTCGAA	1 cut(s)	1841
BspTI	CTTAAG	1 cut(s)	436
BspTNI	GGTCTC	1 cut(s)	701
BsrDI	GCAATG	2 cut(s)	985, 1751
BsrFI	RCCGGY	2 cut(s)	53, 293
BsrGI	TGTACA	3 cut(s)	326, 342, 699
BsrI	ACTGG	3 cut(s)	889, 1728, 2131
BssAI	RCCGGY	2 cut(s)	53, 293
BssECI	CCNNGG	3 cut(s)	403, 597, 1617
BssMI	GATC	9 cut(s)	3, 268, 385, 640, 1081, 1099, 1486, 1506, 1972
Bst2UI	CCWGG	4 cut(s)	728, 1256, 1535, 1619
Bst6I	CTCTTC	5 cut(s)	21, 1056, 1488, 1591, 2012
BstAFI	CTTAAG	1 cut(s)	436
BstAUI	TGTACA	3 cut(s)	326, 342, 699
BstBAI	YACGTR	2 cut(s)	425, 638
BstBI	TTCGAA	1 cut(s)	1841
BstDSI	CCRYGG	1 cut(s)	403
BstENI	CCTNNNNNAGG	1 cut(s)	731
BstF5I	GGATG	7 cut(s)	379, 582, 757, 1402, 1530, 1896, 2044
BstFNI	CGCG	4 cut(s)	116, 252, 1251, 1552
BstHHI	GCGC	2 cut(s)	118, 252
BstKTI	GATC	9 cut(s)	6, 271, 388, 643, 1084, 1102, 1489, 1509, 1975
BstMAI	GTCTC	3 cut(s)	105, 701, 1748
BstMBI	GATC	9 cut(s)	3, 268, 385, 640, 1081, 1099, 1486, 1506, 1972
BstMWI	GCNNNNNNNGC	6 cut(s)	50, 145, 286, 1757, 1991, 2090
BstNI	CCWGG	4 cut(s)	728, 1256, 1535, 1619
BstNSI	RCATGY	2 cut(s)	1018, 1898
BstSCI	CCNGG	6 cut(s)	596, 597, 726, 1254, 1533, 1617
BstSFI	CTRYAG	1 cut(s)	2277
BstSLI	GKGCMC	1 cut(s)	1077
BstUI	CGCG	4 cut(s)	116, 252, 1251, 1552
BstV1I	GCAGC	4 cut(s)	53, 242, 904, 2077
BstX2I	RGATCY	1 cut(s)	385
BstXI	CCANNNNNNTGG	1 cut(s)	727
BstYI	RGATCY	1 cut(s)	385
Bsu15I	ATCGAT	1 cut(s)	267
BsuI	GTATCC	1 cut(s)	1912
BsuRI	GGCC	2 cut(s)	57, 2261
BsuTUI	ATCGAT	1 cut(s)	267
BtgI	CCRYGG	1 cut(s)	403
BtrI	CACGTC	1 cut(s)	664
BtsCI	GGATG	7 cut(s)	379, 582, 757, 1402, 1530, 1896, 2044
BtsI	GCAGTG	1 cut(s)	2300
BtsIMutI	CAGTG	5 cut(s)	510, 549, 1982, 2124, 2300
BveI	ACCTGC	1 cut(s)	724
CaiI	CAGNNNCTG	2 cut(s)	139, 1718
CfoI	GCGC	2 cut(s)	118, 252
Cfr10I	RCCGGY	2 cut(s)	53, 293
Cfr13I	GGNCC	3 cut(s)	153, 590, 1544
Cfr9I	CCCGGG	1 cut(s)	597
ClaI	ATCGAT	1 cut(s)	267
CseI	GACGC	4 cut(s)	122, 530, 1240, 1558
CspCI	CAANNNNNGTGG	2 cut(s)	1155, 1190
CviAII	CATG	6 cut(s)	1015, 1070, 1288, 1331, 1895, 2032
DpnI	GATC	9 cut(s)	5, 270, 387, 642, 1083, 1101, 1488, 1508, 1974
DpnII	GATC	9 cut(s)	3, 268, 385, 640, 1081, 1099, 1486, 1506, 1972
DraI	TTTAAA	2 cut(s)	1962, 2293
Eam1104I	CTCTTC	5 cut(s)	21, 1056, 1488, 1591, 2012
EarI	CTCTTC	5 cut(s)	21, 1056, 1488, 1591, 2012
Ecl136II	GAGCTC	2 cut(s)	89, 148
Eco24I	GRGCYC	3 cut(s)	91, 150, 1115
Eco31I	GGTCTC	1 cut(s)	701
Eco47I	GGWCC	3 cut(s)	153, 590, 1544
Eco53kI	GAGCTC	2 cut(s)	89, 148
Eco57I	CTGAAG	3 cut(s)	839, 1512, 2193
Eco88I	CYCGRG	1 cut(s)	597
EcoICRI	GAGCTC	2 cut(s)	89, 148
EcoNI	CCTNNNNNAGG	1 cut(s)	731
EcoO109I	RGGNCCY	1 cut(s)	590
EcoRI	GAATTC	2 cut(s)	1259, 1793
EcoRII	CCWGG	4 cut(s)	726, 1254, 1533, 1617
EcoT22I	ATGCAT	2 cut(s)	1648, 1896
EcoT38I	GRGCYC	3 cut(s)	91, 150, 1115
Esp3I	CGTCTC	1 cut(s)	105
FaeI	CATG	6 cut(s)	1018, 1073, 1291, 1334, 1898, 2035
FaqI	GGGAC	5 cut(s)	385, 479, 1414, 1553, 1891
FatI	CATG	6 cut(s)	1014, 1069, 1287, 1330, 1894, 2031
FauI	CCCGC	1 cut(s)	189
FauNDI	CATATG	1 cut(s)	1511
FbaI	TGATCA	2 cut(s)	1099, 1972
FblI	GTMKAC	1 cut(s)	2016
Fnu4HI	GCNGC	5 cut(s)	42, 253, 256, 918, 2091
FokI	GGATG	7 cut(s)	386, 589, 764, 1409, 1517, 1903, 2051
FriOI	GRGCYC	3 cut(s)	91, 150, 1115
Fsp4HI	GCNGC	5 cut(s)	42, 253, 256, 918, 2091
FspBI	CTAG	2 cut(s)	309, 1577
GlaI	GCGC	2 cut(s)	117, 251
GluI	GCNGC	5 cut(s)	42, 253, 256, 918, 2091
GsuI	CTGGAG	2 cut(s)	749, 1868
HaeIII	GGCC	2 cut(s)	57, 2261
HapII	CCGG	4 cut(s)	54, 151, 294, 598
HgaI	GACGC	4 cut(s)	122, 530, 1240, 1558
HhaI	GCGC	2 cut(s)	118, 252
Hin1II	CATG	6 cut(s)	1018, 1073, 1291, 1334, 1898, 2035
Hin6I	GCGC	2 cut(s)	116, 250
HinP1I	GCGC	2 cut(s)	116, 250
HincII	GTYRAC	2 cut(s)	1012, 2017
HindII	GTYRAC	2 cut(s)	1012, 2017
HindIII	AAGCTT	4 cut(s)	161, 960, 1232, 1955
HpaII	CCGG	4 cut(s)	54, 151, 294, 598
HphI	GGTGA	5 cut(s)	1006, 1054, 1792, 1864, 2357
Hpy166II	GTNNAC	4 cut(s)	343, 1012, 1075, 2017
Hpy8I	GTNNAC	4 cut(s)	343, 1012, 1075, 2017
Hpy99I	CGWCG	1 cut(s)	1552
HpyAV	CCTTC	7 cut(s)	82, 491, 674, 1342, 1960, 2029, 2176
HpyCH4IV	ACGT	4 cut(s)	424, 637, 663, 828
HpyF10VI	GCNNNNNNNGC	6 cut(s)	50, 145, 286, 1757, 1991, 2090
HpySE526I	ACGT	4 cut(s)	424, 637, 663, 828
Hsp92II	CATG	6 cut(s)	1018, 1073, 1291, 1334, 1898, 2035
HspAI	GCGC	2 cut(s)	116, 250
Kpn2I	TCCGGA	1 cut(s)	150
KroI	GCCGGC	2 cut(s)	53, 293
KroNI	GCCGGC	2 cut(s)	55, 295
Ksp22I	TGATCA	2 cut(s)	1099, 1972
Kzo9I	GATC	9 cut(s)	3, 268, 385, 640, 1081, 1099, 1486, 1506, 1972
LmnI	GCTCC	8 cut(s)	145, 153, 263, 367, 543, 730, 1198, 1441
Lsp1109I	GCAGC	4 cut(s)	53, 242, 904, 2077
LweI	GCATC	7 cut(s)	126, 493, 567, 1633, 1655, 1881, 2272
MaeI	CTAG	2 cut(s)	309, 1577
MaeII	ACGT	4 cut(s)	424, 637, 663, 828
MaeIII	GTNAC	6 cut(s)	443, 1033, 1219, 1852, 2134, 2233
MalI	GATC	9 cut(s)	5, 270, 387, 642, 1083, 1101, 1488, 1508, 1974
MboI	GATC	9 cut(s)	3, 268, 385, 640, 1081, 1099, 1486, 1506, 1972
MfeI	CAATTG	1 cut(s)	742
MflI	RGATCY	1 cut(s)	385
MhlI	GDGCHC	6 cut(s)	91, 150, 540, 1077, 1115, 1195
MluI	ACGCGT	1 cut(s)	1550
MlyI	GAGTC	8 cut(s)	89, 732, 1396, 1675, 1760, 1873, 2249, 2366
MmeI	TCCRAC	3 cut(s)	85, 123, 1519
Mph1103I	ATGCAT	2 cut(s)	1648, 1896
MroI	TCCGGA	1 cut(s)	150
MroNI	GCCGGC	2 cut(s)	53, 293
MroXI	GAANNNNTTC	1 cut(s)	71
MslI	CAYNNNNRTG	2 cut(s)	554, 2366
MspA1I	CMGCKG	1 cut(s)	44
MspCI	CTTAAG	1 cut(s)	436
MspI	CCGG	4 cut(s)	54, 151, 294, 598
MspR9I	CCNGG	6 cut(s)	598, 599, 728, 1256, 1535, 1619
MunI	CAATTG	1 cut(s)	742
Mva1269I	GAATGC	1 cut(s)	408
MvaI	CCWGG	4 cut(s)	728, 1256, 1535, 1619
MvnI	CGCG	4 cut(s)	116, 252, 1251, 1552
MwoI	GCNNNNNNNGC	6 cut(s)	50, 145, 286, 1757, 1991, 2090
NaeI	GCCGGC	2 cut(s)	55, 295
NciI	CCSGG	2 cut(s)	598, 599
NdeI	CATATG	1 cut(s)	1511
NdeII	GATC	9 cut(s)	3, 268, 385, 640, 1081, 1099, 1486, 1506, 1972
NgoMIV	GCCGGC	2 cut(s)	53, 293
NlaIII	CATG	6 cut(s)	1018, 1073, 1291, 1334, 1898, 2035
NlaIV	GGNNCC	3 cut(s)	591, 1779, 2189
NmeAIII	GCCGAG	3 cut(s)	226, 2006, 2237
NmuCI	GTSAC	4 cut(s)	1033, 1219, 1852, 2233
NsiI	ATGCAT	2 cut(s)	1648, 1896
NspI	RCATGY	2 cut(s)	1018, 1898
NspV	TTCGAA	1 cut(s)	1841
PctI	GAATGC	1 cut(s)	408
PdiI	GCCGGC	2 cut(s)	55, 295
PdmI	GAANNNNTTC	1 cut(s)	71
PfeI	GAWTC	5 cut(s)	48, 415, 1627, 1838, 2079
PflFI	GACNNNGTC	1 cut(s)	512
PflMI	CCANNNNNTGG	1 cut(s)	895
PfoI	TCCNGGA	2 cut(s)	726, 1254
PkrI	GCNGC	5 cut(s)	43, 254, 257, 919, 2092
PleI	GAGTC	8 cut(s)	89, 731, 1396, 1675, 1760, 1873, 2249, 2365
PpsI	GAGTC	8 cut(s)	89, 731, 1396, 1675, 1760, 1873, 2249, 2365
Ppu21I	YACGTR	2 cut(s)	425, 638
PpuMI	RGGWCCY	1 cut(s)	590
PshBI	ATTAAT	1 cut(s)	1370
PsiI	TTATAA	1 cut(s)	1301
Psp124BI	GAGCTC	2 cut(s)	91, 150
Psp5II	RGGWCCY	1 cut(s)	590
Psp6I	CCWGG	4 cut(s)	726, 1254, 1533, 1617
PspGI	CCWGG	4 cut(s)	726, 1254, 1533, 1617
PspN4I	GGNNCC	3 cut(s)	591, 1779, 2189
PspPI	GGNCC	3 cut(s)	153, 590, 1544
PspPPI	RGGWCCY	1 cut(s)	590
PsrI	GAACNNNNNNTAC	8 cut(s)	319, 351, 1713, 1745, 2113, 2145, 2300, 2332
PstNI	CAGNNNCTG	2 cut(s)	139, 1718
PsuI	RGATCY	1 cut(s)	385
PsyI	GACNNNGTC	1 cut(s)	512
PvuII	CAGCTG	1 cut(s)	44
RseI	CAYNNNNRTG	2 cut(s)	554, 2366
SacI	GAGCTC	2 cut(s)	91, 150
SalI	GTCGAC	1 cut(s)	2015
SatI	GCNGC	5 cut(s)	42, 253, 256, 918, 2091
Sau3AI	GATC	9 cut(s)	3, 268, 385, 640, 1081, 1099, 1486, 1506, 1972
Sau96I	GGNCC	3 cut(s)	153, 590, 1544
ScaI	AGTACT	2 cut(s)	125, 179
SchI	GAGTC	8 cut(s)	89, 732, 1396, 1675, 1760, 1873, 2249, 2366
ScrFI	CCNGG	6 cut(s)	598, 599, 728, 1256, 1535, 1619
SduI	GDGCHC	6 cut(s)	91, 150, 540, 1077, 1115, 1195
SfaNI	GCATC	7 cut(s)	126, 493, 567, 1633, 1655, 1881, 2272
SfcI	CTRYAG	1 cut(s)	2277
SfuI	TTCGAA	1 cut(s)	1841
SinI	GGWCC	3 cut(s)	153, 590, 1544
SmaI	CCCGGG	1 cut(s)	599
SmiMI	CAYNNNNRTG	2 cut(s)	554, 2366
SmlI	CTYRAG	4 cut(s)	119, 436, 453, 1199
SmoI	CTYRAG	4 cut(s)	119, 436, 453, 1199
SsiI	CCGC	4 cut(s)	196, 252, 574, 2305
SspMI	CTAG	2 cut(s)	309, 1577
SstI	GAGCTC	2 cut(s)	91, 150
StyD4I	CCNGG	6 cut(s)	596, 597, 726, 1254, 1533, 1617
TaiI	ACGT	4 cut(s)	427, 640, 666, 831
TaqI	TCGA	8 cut(s)	98, 158, 267, 1084, 1353, 1841, 2016, 2178
TaqII	GACCGA	1 cut(s)	1561
TatI	WGTACW	8 cut(s)	123, 177, 326, 342, 699, 926, 955, 1514
TauI	GCSGC	1 cut(s)	255
TfiI	GAWTC	5 cut(s)	48, 415, 1627, 1838, 2079
TscAI	CASTG	5 cut(s)	517, 556, 1982, 2131, 2307
TseFI	GTSAC	4 cut(s)	1033, 1219, 1852, 2233
TseI	GCWGC	4 cut(s)	41, 255, 917, 2090
Tsp45I	GTSAC	4 cut(s)	1033, 1219, 1852, 2233
TspDTI	ATGAA	9 cut(s)	39, 510, 572, 1035, 1331, 1437, 1806, 1853, 2048
TspGWI	ACGGA	1 cut(s)	27
TspMI	CCCGGG	1 cut(s)	597
TspRI	CASTG	5 cut(s)	517, 556, 1982, 2131, 2307
Tth111I	GACNNNGTC	1 cut(s)	512
Van91I	CCANNNNNTGG	1 cut(s)	895
Vha464I	CTTAAG	1 cut(s)	436
VneI	GTGCAC	1 cut(s)	1073
VpaK11BI	GGWCC	3 cut(s)	153, 590, 1544
VspI	ATTAAT	1 cut(s)	1370
XagI	CCTNNNNNAGG	1 cut(s)	731
XapI	RAATTY	8 cut(s)	544, 1058, 1146, 1259, 1447, 1665, 1793, 1830
XceI	RCATGY	2 cut(s)	1018, 1898
XmaI	CCCGGG	1 cut(s)	597
XmiI	GTMKAC	1 cut(s)	2016
XmnI	GAANNNNTTC	1 cut(s)	71
XspI	CTAG	2 cut(s)	309, 1577
ZrmI	AGTACT	2 cut(s)	125, 179
Zsp2I	ATGCAT	2 cut(s)	1648, 1896

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.