Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0011815

Physical Location & Seq

Genomic Coordinates Reverse (-)

81517951 .. 81525794

Gene Structure 7844 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00143530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2259 bp

ATGATCTCCGACGGAGTTGAAGACGAAGAGAAGTACCTGGCGGCCGGAATCGCCGGACTTCAACAGAATGCCTTCTACATGCATCGGGCTCTGGACTCCAACAATCTCAGGGACGCTCTCAAGTACTCGGCTCAGATGCTATCGGAGCTCCGCACCTCCAAGCTTTCGCCTCACAAATACTACGAGCTCTATATGCGAGCGTTTGATGAATTGAGGAAATTGGAGATGTTCTTCAAGGAGGAGGCTCGGCGCGGTTGCTCTATTATTGATCTTTATGAGCTTGTGCAGCATGCTGGTAACATATTGCCCAGATTATATCTTCTGTGTACAGTAGGATCTGTTTACATCAAATCTAAGGAAGCTCCTGCTAAGGATGTCCTGAAAGATCTTGTGGAAATGTCACGTGGCATTCAGAATCCTGTACGTGGGCTCTTTTTAAGGAGTTACCTTTCTCAAGTCAGTAGGGATAAGTTGCCTGATATTGGGTCGGAGTATGAAGGGGATGCAGATACTGTCAGGGATGCTGTTGAGTTTGTTCTCCAAAACTTCACTGAGATGAACAAGCTTTGGGTGCGGATGCAACATCAGGGGCCTGCTAGGGAAAAGGAGAAACGGGAGAAAGAAAGGAGCGAGCTACGTGATCTTGTTGGTAAAAACCTGCATGTTCTCAGTCAGATAGAAGGTGTTGATCTGGATTTGTACAAAGAGACCGTGCTTCCCAGAGTTCTGGAGCAGCCATCTGTTGATATCAAGACAGTGCTATCCCAGCTAATGGAAAGGCTTTCCAACTATGCTGCCTCAAGCATAGAAGTGTTGCCCGAGTTCTTACAAGTAGAAGCTTTTTCTAAACTGAGCAATGCGATTGGAAAGGTGATTGAAGCGCACGTTGACATGCCTGTAATTGGTGTTGTTACTTTGTACTCATCCCTTCTTAAATTTACTCTCCATGTGCATCCGGATCGACTTGATTATGCTGATCAAGTGTTGGGGTCATTTGTTAAGAAGCTCTCTGGAAAAGGAAAAATTGAAGACAGCAGGGCAACAAAACAAGTTGTTGCACTTTTAAGTGCTCCATTGGAGAAATATAATGATATTGTGACTGCTTTGAAGCTTACAAATTATCCCCGTGTCATGGAATTCCTTGATACTGGAACAAATAAAGTCATGGCAACTGTTATAATACAAAGCATTATGAAAAATACAACTCATATATTAACTGCTGAGAAGGTCGAGGCATTGTTTGAATTAATAAAAGGACTTATCGAGGATTTGGATGGGACTCTTCATGATGAGGTTGATGAAGATGATTTCAAGGAGGAGCAGAATTCTGTTGCGAGACTTATTCAAATGTTCCATAATGAAGATCCAGAAGAGATGTTTAAGATCATAGGTACTGTGCAGAAGCACATCATGTCTGGAGGACCAAAGCGCCTACCCTTTACTGTCCCTCCCCTAGTCTTTAATTCTCTAAAGTTGGTCAGGCAATTGCAAGGAGGACATGAAGATAAAGAAAATCCTTTTGGAGAAGAAGCGTCGACTACACCAAAGAAAATTTTTCAGCTTTTGTCTCAGACTATTGAGGCTCTGTTGAATGTGCCAGCTCCTGAGCTAGCTTTACGGCTGTACTTGCAATGTGCTGAGGCTGCAAATGACTGTGATTTAGAACCTGTTGCATACGAATTCTTCACCCAAGCATATATTCTCTATGAAGAAGAAATTTCAGATTCGAAAGCACAGGTGACTGCAATTCATTTGATAATTGGGACTCTCCAGAGGATGCATGTATTTGGTGTTGAGAACAGAGATACTTTAACTCACAAAGCCACAGGGTATTCGGCAAAGCTTTTAAAGAAGCCTGATCAGTGCCGAGCTGTTTATGCTTGCTCGCATCTTTTCTGGGTTGACGATCAGGAGAACATGAAGGATGGGGAAAGGGTACTGATTTGCCTAAAGAGAGCTTTAAGGATTGCAAACGCTGCCCAGCAGATGGCCAACGCAACCCGTGGTAGCACCGGTTCAGCGACACTCTTCGTTGAAATACTGAACAAGTACCTTTATTTCTTTGAGAAGGGCAACCCACAGATAACTGTTGCTTCTGTACAAAGCCTGATTGAATTGATTACTACTGAAATGCAAAGTGACTCAACCCCAGCAGAATCGGCTACAAATGCATTCTTTGCCAGCACGATGCGGTACATTCAATTCCAGAAGCAGAAAGGTGGCGCAGTTGGTGAAAAATATGAGCAAATCAAGGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008565 GO:0010008 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0016197 GO:0016482 GO:0030904 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042147 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

Vps35

Protein Analysis

752

Amino Acids

85.01

Weight (kDa)

5.57

Isoelectric Point (pI)

42.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps35	PF03635	13 - 245	2.9e-118	Vacuolar protein sorting-associated protein 35
Vps35	PF03635	245 - 709	3.2e-135	Vacuolar protein sorting-associated protein 35

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011815)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17790 AT3G51310
fragaria_vesca	FvH4_1g11410
malus_domestica	MD02G1126400.v1.1 MD15G1242400.v1.1
prunus_persica	Prupe.7G171800_v2.0.a1 Prupe.7G171800_v2.0.a1
pyrus_communis	pycom02g09870 pycom15g21360
rosa_chinensis	RchiOBHm_Chr2g0098981
rosa_laevigata	RLG00000016838
rosa_multiflora	Rmu_sc0000577.1_g000009
rosa_roxburghii	Rroxscaffold_2G00143530
rosa_rugosa	Rorug02G0075400
rosa_samantha	Rh2AG124900 Rh2BG128100 Rh2CG129000 Rh2DG129200
rosa_wichuraiana	Rw2G009590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1178
Acc36I	ACCTGC	1 cut(s)	666
AccB7I	CCANNNNNTGG	2 cut(s)	772, 1987
AccI	GTMKAC	1 cut(s)	1535
AccII	CGCG	1 cut(s)	252
AccIII	TCCGGA	1 cut(s)	955
AciI	CCGC	5 cut(s)	41, 151, 252, 574, 2191
AclWI	GGATC	3 cut(s)	343, 966, 1358
AcoI	YGGCCR	2 cut(s)	42, 1989
AcsI	RAATTY	6 cut(s)	935, 1136, 1324, 1551, 1679, 1716
AcvI	CACGTG	1 cut(s)	404
AfiI	CCNNNNNNNGG	6 cut(s)	425, 482, 772, 902, 1132, 1987
AgeI	ACCGGT	1 cut(s)	2012
AjnI	CCWGG	1 cut(s)	36
AjuI	GAANNNNNNNTTGG	4 cut(s)	1503, 1535, 1683, 1715
Alw21I	GWGCWC	3 cut(s)	150, 189, 1072
Alw26I	GTCTC	3 cut(s)	701, 1330, 1572
AlwI	GGATC	3 cut(s)	343, 966, 1358
AlwNI	CAGNNNCTG	2 cut(s)	512, 1604
Ama87I	CYCGRG	1 cut(s)	818
Aor13HI	TCCGGA	1 cut(s)	955
AoxI	GGCC	3 cut(s)	42, 590, 1989
ApeKI	GCWGC	5 cut(s)	286, 733, 794, 1643, 1976
ApoI	RAATTY	6 cut(s)	935, 1136, 1324, 1551, 1679, 1716
AseI	ATTAAT	1 cut(s)	1247
AsiGI	ACCGGT	1 cut(s)	2012
Asp700I	GAANNNNTTC	1 cut(s)	71
AspLEI	GCGC	4 cut(s)	252, 883, 1431, 2225
AspS9I	GGNCC	2 cut(s)	590, 1421
AsuHPI	GGTGA	4 cut(s)	883, 1678, 1750, 2243
AsuII	TTCGAA	1 cut(s)	1727
AsuNHI	GCTAGC	1 cut(s)	1609
AvaI	CYCGRG	1 cut(s)	818
AvaII	GGWCC	1 cut(s)	1421
BalI	TGGCCA	1 cut(s)	1991
BanII	GRGCYC	4 cut(s)	91, 150, 189, 432
BbrPI	CACGTG	1 cut(s)	404
BbsI	GAAGAC	2 cut(s)	27, 1035
Bbv12I	GWGCWC	3 cut(s)	150, 189, 1072
BbvCI	CCTCAGC	1 cut(s)	1638
BbvI	GCAGC	5 cut(s)	298, 745, 781, 1630, 1963
BccI	CCATC	4 cut(s)	745, 1268, 1919, 1981
BceAI	ACGGC	1 cut(s)	1634
BcgI	CGANNNNNNTGC	2 cut(s)	941, 975
BciT130I	CCWGG	1 cut(s)	38
BclI	TGATCA	2 cut(s)	976, 1858
BcoDI	GTCTC	3 cut(s)	701, 1330, 1572
BfaI	CTAG	3 cut(s)	597, 1454, 1610
BfoI	RGCGCY	1 cut(s)	1432
BfuAI	ACCTGC	1 cut(s)	666
BglII	AGATCT	1 cut(s)	385
BisI	GCNGC	6 cut(s)	42, 287, 734, 795, 1644, 1977
BlsI	GCNGC	6 cut(s)	43, 288, 735, 796, 1645, 1978
BmcAI	AGTACT	1 cut(s)	125
Bme1390I	CCNGG	1 cut(s)	38
Bme18I	GGWCC	1 cut(s)	1421
BmeT110I	CYCGRG	1 cut(s)	818
BmgT120I	GGNCC	2 cut(s)	590, 1421
BmiI	GGNNCC	1 cut(s)	591
BmrFI	CCNGG	1 cut(s)	38
BmsI	GCATC	9 cut(s)	91, 126, 493, 511, 567, 961, 1767, 1897, 2178
BmtI	GCTAGC	1 cut(s)	1613
BpiI	GAAGAC	2 cut(s)	27, 1035
BpmI	CTGGAG	3 cut(s)	749, 1437, 1754
Bpu10I	CCTNAGC	3 cut(s)	369, 1605, 1638
Bpu14I	TTCGAA	1 cut(s)	1727
BpuEI	CTTGAG	3 cut(s)	104, 438, 784
BsaAI	YACGTR	3 cut(s)	404, 425, 638
BsaI	GGTCTC	1 cut(s)	701
BsaJI	CCNNGG	1 cut(s)	2002
BsaWI	WCCGGW	2 cut(s)	955, 2012
BsaXI	ACNNNNNCTCC	2 cut(s)	1432, 1462
Bsc4I	CCNNNNNNNGG	6 cut(s)	425, 482, 772, 902, 1132, 1987
Bse118I	RCCGGY	1 cut(s)	2012
Bse1I	ACTGG	1 cut(s)	1153
Bse3DI	GCAATG	2 cut(s)	862, 1637
BseAI	TCCGGA	1 cut(s)	955
BseBI	CCWGG	1 cut(s)	38
BseDI	CCNNGG	1 cut(s)	2002
BseGI	GGATG	9 cut(s)	379, 508, 526, 582, 923, 952, 1279, 1782, 1930
BseLI	CCNNNNNNNGG	6 cut(s)	425, 482, 772, 902, 1132, 1987
BseMI	GCAATG	2 cut(s)	862, 1637
BseMII	CTCAG	9 cut(s)	121, 146, 543, 682, 842, 1212, 1583, 1596, 1629
BseNI	ACTGG	1 cut(s)	1153
BseRI	GAGGAG	2 cut(s)	254, 1331
BseX3I	CGGCCG	1 cut(s)	42
BseXI	GCAGC	5 cut(s)	298, 745, 781, 1630, 1963
BseYI	CCCAGC	3 cut(s)	765, 1980, 2149
BsgI	GTGCAG	2 cut(s)	305, 1418
Bsh1236I	CGCG	1 cut(s)	252
Bsh1285I	CGRYCG	1 cut(s)	45
BshFI	GGCC	3 cut(s)	44, 592, 1991
BshTI	ACCGGT	1 cut(s)	2012
BsiEI	CGRYCG	1 cut(s)	45
BsiHKAI	GWGCWC	3 cut(s)	150, 189, 1072
BsiHKCI	CYCGRG	1 cut(s)	818
BsiSI	CCGG	4 cut(s)	45, 54, 956, 2013
BslFI	GGGAC	4 cut(s)	125, 1291, 1430, 1777
BslI	CCNNNNNNNGG	6 cut(s)	425, 482, 772, 902, 1132, 1987
BsmAI	GTCTC	3 cut(s)	701, 1330, 1572
BsmFI	GGGAC	4 cut(s)	125, 1291, 1430, 1777
BsmI	GAATGC	3 cut(s)	73, 408, 2171
BsnI	GGCC	3 cut(s)	44, 592, 1991
Bso31I	GGTCTC	1 cut(s)	701
BsoBI	CYCGRG	1 cut(s)	818
Bsp119I	TTCGAA	1 cut(s)	1727
Bsp1286I	GDGCHC	5 cut(s)	91, 150, 189, 432, 1072
Bsp13I	TCCGGA	1 cut(s)	955
Bsp1407I	TGTACA	3 cut(s)	326, 699, 2098
BspACI	CCGC	5 cut(s)	41, 151, 252, 574, 2191
BspANI	GGCC	3 cut(s)	44, 592, 1991
BspCNI	CTCAG	9 cut(s)	120, 145, 544, 681, 843, 1213, 1582, 1597, 1630
BspEI	TCCGGA	1 cut(s)	955
BspFNI	CGCG	1 cut(s)	252
BspHI	TCATGA	1 cut(s)	1285
BspLI	GGNNCC	1 cut(s)	591
BspMI	ACCTGC	1 cut(s)	666
BspOI	GCTAGC	1 cut(s)	1613
BspPI	GGATC	3 cut(s)	343, 966, 1358
BspT104I	TTCGAA	1 cut(s)	1727
BspTNI	GGTCTC	1 cut(s)	701
BsrDI	GCAATG	2 cut(s)	862, 1637
BsrFI	RCCGGY	1 cut(s)	2012
BsrGI	TGTACA	3 cut(s)	326, 699, 2098
BsrI	ACTGG	1 cut(s)	1153
BssAI	RCCGGY	1 cut(s)	2012
BssECI	CCNNGG	1 cut(s)	2002
Bst2UI	CCWGG	1 cut(s)	38
Bst4CI	ACNGT	9 cut(s)	331, 514, 712, 757, 1174, 1396, 1444, 1655, 2089
Bst6I	CTCTTC	4 cut(s)	21, 1287, 1365, 2033
BstAPI	GCANNNNNTGC	2 cut(s)	1976, 2177
BstAUI	TGTACA	3 cut(s)	326, 699, 2098
BstBAI	YACGTR	3 cut(s)	404, 425, 638
BstBI	TTCGAA	1 cut(s)	1727
BstC8I	GCNNGC	9 cut(s)	198, 291, 594, 632, 1599, 1611, 1882, 1886, 2182
BstDSI	CCRYGG	1 cut(s)	2002
BstF5I	GGATG	9 cut(s)	379, 508, 526, 582, 923, 952, 1279, 1782, 1930
BstFNI	CGCG	1 cut(s)	252
BstH2I	RGCGCY	1 cut(s)	1432
BstHHI	GCGC	4 cut(s)	252, 883, 1431, 2225
BstMAI	GTCTC	3 cut(s)	701, 1330, 1572
BstMCI	CGRYCG	1 cut(s)	45
BstNI	CCWGG	1 cut(s)	38
BstNSI	RCATGY	5 cut(s)	82, 293, 665, 895, 1784
BstSCI	CCNGG	1 cut(s)	36
BstUI	CGCG	1 cut(s)	252
BstV1I	GCAGC	5 cut(s)	298, 745, 781, 1630, 1963
BstV2I	GAAGAC	2 cut(s)	27, 1035
BstX2I	RGATCY	3 cut(s)	335, 385, 1363
BstXI	CCANNNNNNTGG	1 cut(s)	727
BstYI	RGATCY	3 cut(s)	335, 385, 1363
BstZI	CGGCCG	1 cut(s)	42
BsuRI	GGCC	3 cut(s)	44, 592, 1991
BtgI	CCRYGG	1 cut(s)	2002
BtsCI	GGATG	9 cut(s)	379, 508, 526, 582, 923, 952, 1279, 1782, 1930
BtsIMutI	CAGTG	3 cut(s)	549, 762, 1868
BveI	ACCTGC	1 cut(s)	666
Cac8I	GCNNGC	9 cut(s)	198, 291, 594, 632, 1599, 1611, 1882, 1886, 2182
CaiI	CAGNNNCTG	2 cut(s)	512, 1604
CciI	TCATGA	1 cut(s)	1285
CfoI	GCGC	4 cut(s)	252, 883, 1431, 2225
Cfr10I	RCCGGY	1 cut(s)	2012
Cfr13I	GGNCC	2 cut(s)	590, 1421
CseI	GACGC	2 cut(s)	122, 1521
CspAI	ACCGGT	1 cut(s)	2012
DraI	TTTAAA	1 cut(s)	1848
EaeI	YGGCCR	2 cut(s)	42, 1989
EagI	CGGCCG	1 cut(s)	42
Eam1104I	CTCTTC	4 cut(s)	21, 1287, 1365, 2033
EarI	CTCTTC	4 cut(s)	21, 1287, 1365, 2033
Ecl136II	GAGCTC	2 cut(s)	148, 187
EclXI	CGGCCG	1 cut(s)	42
Eco24I	GRGCYC	4 cut(s)	91, 150, 189, 432
Eco31I	GGTCTC	1 cut(s)	701
Eco32I	GATATC	1 cut(s)	748
Eco47I	GGWCC	1 cut(s)	1421
Eco52I	CGGCCG	1 cut(s)	42
Eco53kI	GAGCTC	2 cut(s)	148, 187
Eco72I	CACGTG	1 cut(s)	404
Eco88I	CYCGRG	1 cut(s)	818
EcoICRI	GAGCTC	2 cut(s)	148, 187
EcoO109I	RGGNCCY	1 cut(s)	590
EcoRI	GAATTC	3 cut(s)	1136, 1324, 1679
EcoRII	CCWGG	1 cut(s)	36
EcoRV	GATATC	1 cut(s)	748
EcoT22I	ATGCAT	3 cut(s)	84, 1782, 2173
EcoT38I	GRGCYC	4 cut(s)	91, 150, 189, 432
FaqI	GGGAC	4 cut(s)	125, 1291, 1430, 1777
FbaI	TGATCA	2 cut(s)	976, 1858
FblI	GTMKAC	1 cut(s)	1535
Fnu4HI	GCNGC	6 cut(s)	42, 287, 734, 795, 1644, 1977
FokI	GGATG	9 cut(s)	386, 515, 533, 589, 910, 939, 1286, 1789, 1937
FriOI	GRGCYC	4 cut(s)	91, 150, 189, 432
Fsp4HI	GCNGC	6 cut(s)	42, 287, 734, 795, 1644, 1977
FspBI	CTAG	3 cut(s)	597, 1454, 1610
GlaI	GCGC	4 cut(s)	251, 882, 1430, 2224
GluI	GCNGC	6 cut(s)	42, 287, 734, 795, 1644, 1977
GsaI	CCCAGC	3 cut(s)	769, 1984, 2153
GsuI	CTGGAG	3 cut(s)	749, 1437, 1754
HaeII	RGCGCY	1 cut(s)	1432
HaeIII	GGCC	3 cut(s)	44, 592, 1991
HapII	CCGG	4 cut(s)	45, 54, 956, 2013
HgaI	GACGC	2 cut(s)	122, 1521
HhaI	GCGC	4 cut(s)	252, 883, 1431, 2225
Hin6I	GCGC	4 cut(s)	250, 881, 1429, 2223
HinP1I	GCGC	4 cut(s)	250, 881, 1429, 2223
HincII	GTYRAC	3 cut(s)	889, 1536, 1903
HindII	GTYRAC	3 cut(s)	889, 1536, 1903
HindIII	AAGCTT	5 cut(s)	161, 563, 837, 1109, 1841
HinfI	GANTC	8 cut(s)	48, 95, 415, 1279, 1724, 1765, 2141, 2156
HpaII	CCGG	4 cut(s)	45, 54, 956, 2013
HphI	GGTGA	4 cut(s)	883, 1678, 1750, 2243
Hpy166II	GTNNAC	5 cut(s)	327, 343, 889, 1536, 1903
Hpy188I	TCNGA	8 cut(s)	10, 135, 145, 414, 490, 675, 1572, 1723
Hpy8I	GTNNAC	5 cut(s)	327, 343, 889, 1536, 1903
Hpy99I	CGWCG	2 cut(s)	14, 1537
HpyAV	CCTTC	7 cut(s)	82, 491, 674, 938, 1219, 1915, 2062
HpyCH4III	ACNGT	9 cut(s)	331, 514, 712, 757, 1174, 1396, 1444, 1655, 2089
HpyCH4IV	ACGT	4 cut(s)	403, 424, 637, 885
HpySE526I	ACGT	4 cut(s)	403, 424, 637, 885
HspAI	GCGC	4 cut(s)	250, 881, 1429, 2223
Kpn2I	TCCGGA	1 cut(s)	955
Ksp22I	TGATCA	2 cut(s)	976, 1858
LmnI	GCTCC	8 cut(s)	145, 153, 367, 627, 730, 1075, 1318, 1606
Lsp1109I	GCAGC	5 cut(s)	298, 745, 781, 1630, 1963
LweI	GCATC	9 cut(s)	91, 126, 493, 511, 567, 961, 1767, 1897, 2178
MaeI	CTAG	3 cut(s)	597, 1454, 1610
MaeII	ACGT	4 cut(s)	403, 424, 637, 885
MaeIII	GTNAC	7 cut(s)	296, 399, 443, 910, 1096, 1738, 2138
MfeI	CAATTG	1 cut(s)	1484
MflI	RGATCY	3 cut(s)	335, 385, 1363
MhlI	GDGCHC	5 cut(s)	91, 150, 189, 432, 1072
MlsI	TGGCCA	1 cut(s)	1991
MluNI	TGGCCA	1 cut(s)	1991
MlyI	GAGTC	4 cut(s)	89, 1273, 1759, 2135
MmeI	TCCRAC	4 cut(s)	33, 123, 468, 810
Mox20I	TGGCCA	1 cut(s)	1991
Mph1103I	ATGCAT	3 cut(s)	84, 1782, 2173
MroI	TCCGGA	1 cut(s)	955
MroXI	GAANNNNTTC	1 cut(s)	71
MscI	TGGCCA	1 cut(s)	1991
MslI	CAYNNNNRTG	2 cut(s)	554, 809
Msp20I	TGGCCA	1 cut(s)	1991
MspI	CCGG	4 cut(s)	45, 54, 956, 2013
MspR9I	CCNGG	1 cut(s)	38
MunI	CAATTG	1 cut(s)	1484
Mva1269I	GAATGC	3 cut(s)	73, 408, 2171
MvaI	CCWGG	1 cut(s)	38
MvnI	CGCG	1 cut(s)	252
NheI	GCTAGC	1 cut(s)	1609
NlaIV	GGNNCC	1 cut(s)	591
NmeAIII	GCCGAG	3 cut(s)	107, 226, 1892
NmuCI	GTSAC	4 cut(s)	399, 1096, 1738, 2138
NsiI	ATGCAT	3 cut(s)	84, 1782, 2173
NspI	RCATGY	5 cut(s)	82, 293, 665, 895, 1784
NspV	TTCGAA	1 cut(s)	1727
PaeI	GCATGC	1 cut(s)	293
PagI	TCATGA	1 cut(s)	1285
PctI	GAATGC	3 cut(s)	73, 408, 2171
PdmI	GAANNNNTTC	1 cut(s)	71
PfeI	GAWTC	4 cut(s)	48, 415, 1724, 2156
PflMI	CCANNNNNTGG	2 cut(s)	772, 1987
PinAI	ACCGGT	1 cut(s)	2012
PkrI	GCNGC	6 cut(s)	43, 288, 735, 796, 1645, 1978
PleI	GAGTC	4 cut(s)	89, 1273, 1759, 2135
PmaCI	CACGTG	1 cut(s)	404
PmlI	CACGTG	1 cut(s)	404
PpsI	GAGTC	4 cut(s)	89, 1273, 1759, 2135
Ppu21I	YACGTR	3 cut(s)	404, 425, 638
PshBI	ATTAAT	1 cut(s)	1247
PsiI	TTATAA	1 cut(s)	1178
Psp124BI	GAGCTC	2 cut(s)	150, 189
Psp6I	CCWGG	1 cut(s)	36
PspCI	CACGTG	1 cut(s)	404
PspFI	CCCAGC	3 cut(s)	765, 1980, 2149
PspGI	CCWGG	1 cut(s)	36
PspN4I	GGNNCC	1 cut(s)	591
PspPI	GGNCC	2 cut(s)	590, 1421
PsrI	GAACNNNNNNTAC	2 cut(s)	1999, 2031
PstNI	CAGNNNCTG	2 cut(s)	512, 1604
PsuI	RGATCY	3 cut(s)	335, 385, 1363
RseI	CAYNNNNRTG	2 cut(s)	554, 809
SacI	GAGCTC	2 cut(s)	150, 189
SalI	GTCGAC	1 cut(s)	1534
SatI	GCNGC	6 cut(s)	42, 287, 734, 795, 1644, 1977
Sau96I	GGNCC	2 cut(s)	590, 1421
ScaI	AGTACT	1 cut(s)	125
SchI	GAGTC	4 cut(s)	89, 1273, 1759, 2135
ScrFI	CCNGG	1 cut(s)	38
SduI	GDGCHC	5 cut(s)	91, 150, 189, 432, 1072
SfaNI	GCATC	9 cut(s)	91, 126, 493, 511, 567, 961, 1767, 1897, 2178
SfuI	TTCGAA	1 cut(s)	1727
SinI	GGWCC	1 cut(s)	1421
SmiMI	CAYNNNNRTG	2 cut(s)	554, 809
SmlI	CTYRAG	3 cut(s)	119, 453, 799
SmoI	CTYRAG	3 cut(s)	119, 453, 799
SphI	GCATGC	1 cut(s)	293
SsiI	CCGC	5 cut(s)	41, 151, 252, 574, 2191
SspMI	CTAG	3 cut(s)	597, 1454, 1610
SstI	GAGCTC	2 cut(s)	150, 189
StyD4I	CCNGG	1 cut(s)	36
TaaI	ACNGT	9 cut(s)	331, 514, 712, 757, 1174, 1396, 1444, 1655, 2089
TaiI	ACGT	4 cut(s)	406, 427, 640, 888
TaqI	TCGA	5 cut(s)	961, 1230, 1263, 1535, 1727
TatI	WGTACW	6 cut(s)	123, 326, 699, 918, 1623, 2098
TauI	GCSGC	1 cut(s)	44
TfiI	GAWTC	4 cut(s)	48, 415, 1724, 2156
TscAI	CASTG	3 cut(s)	556, 762, 1868
TseFI	GTSAC	4 cut(s)	399, 1096, 1738, 2138
TseI	GCWGC	5 cut(s)	286, 733, 794, 1643, 1976
Tsp45I	GTSAC	4 cut(s)	399, 1096, 1738, 2138
TspGWI	ACGGA	1 cut(s)	27
TspRI	CASTG	3 cut(s)	556, 762, 1868
Van91I	CCANNNNNTGG	2 cut(s)	772, 1987
VpaK11BI	GGWCC	1 cut(s)	1421
VspI	ATTAAT	1 cut(s)	1247
XapI	RAATTY	6 cut(s)	935, 1136, 1324, 1551, 1679, 1716
XceI	RCATGY	5 cut(s)	82, 293, 665, 895, 1784
XmiI	GTMKAC	1 cut(s)	1535
XmnI	GAANNNNTTC	1 cut(s)	71
XspI	CTAG	3 cut(s)	597, 1454, 1610
ZrmI	AGTACT	1 cut(s)	125
Zsp2I	ATGCAT	3 cut(s)	84, 1782, 2173

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.